AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene060503

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene060503.t1 MTR_8g024790 94.702 453 22 1 12 462 12 464 0.0 894
MS.gene060503.t1 MTR_8g024770 80.324 432 64 8 38 460 35 454 0.0 687
MS.gene060503.t1 MTR_5g030430 50.990 202 88 4 270 461 474 674 1.96e-55 196
MS.gene060503.t1 MTR_5g030430 32.432 185 92 3 25 180 44 224 3.35e-28 118
MS.gene060503.t1 MTR_8g067280 48.020 202 92 4 271 461 444 643 6.16e-52 186
MS.gene060503.t1 MTR_8g067280 32.057 209 108 7 25 203 53 257 2.75e-25 109
MS.gene060503.t1 MTR_7g096350 28.631 482 241 15 19 440 43 481 4.05e-41 154
MS.gene060503.t1 MTR_7g117670 26.186 527 272 15 25 452 49 557 7.59e-37 142
MS.gene060503.t1 MTR_5g030420 31.720 186 94 4 25 181 27 208 1.09e-28 113
MS.gene060503.t1 MTR_5g030420 31.720 186 94 4 25 181 27 208 1.34e-28 114
MS.gene060503.t1 MTR_8g027495 37.427 171 97 2 283 452 452 613 5.65e-28 117
MS.gene060503.t1 MTR_1g072320 33.526 173 103 3 281 441 457 629 9.31e-19 89.7
MS.gene060503.t1 MTR_1g072320 27.638 199 94 7 19 181 4 188 1.77e-16 82.4
MS.gene060503.t1 MTR_4g097940 30.500 200 111 7 243 426 102 289 6.38e-18 85.1
MS.gene060503.t1 MTR_4g009110 27.363 201 100 5 252 426 133 313 3.78e-17 83.2
MS.gene060503.t1 MTR_3g104100 33.939 165 98 4 277 431 183 346 9.18e-17 82.0
MS.gene060503.t1 MTR_2g104490 28.889 180 114 6 272 441 116 291 1.31e-16 80.5
MS.gene060503.t1 MTR_0250s0040 28.729 181 109 5 289 457 88 260 2.53e-16 79.3
MS.gene060503.t1 MTR_4g009540 30.435 161 95 5 275 426 142 294 3.97e-16 79.3
MS.gene060503.t1 MTR_2g104490 30.000 160 103 5 272 426 116 271 4.76e-16 79.3
MS.gene060503.t1 MTR_4g009540 30.435 161 95 5 275 426 142 294 5.61e-16 79.3
MS.gene060503.t1 MTR_6g047570 32.168 143 90 3 260 401 134 270 7.77e-16 79.0
MS.gene060503.t1 MTR_2g104550 24.375 160 115 2 272 426 117 275 9.08e-16 78.6
MS.gene060503.t1 MTR_5g014600 28.063 253 148 10 183 418 52 287 1.51e-15 78.2
MS.gene060503.t1 MTR_5g014640 33.571 140 83 3 290 426 155 287 1.55e-15 77.8
MS.gene060503.t1 MTR_4g092700 28.161 174 103 4 263 428 137 296 1.80e-15 77.8
MS.gene060503.t1 MTR_1g112680 27.753 227 123 4 241 428 259 483 3.49e-15 78.2
MS.gene060503.t1 MTR_2g022280 26.705 176 115 4 288 451 1 174 3.53e-15 74.3
MS.gene060503.t1 MTR_0011s0260 31.159 138 94 1 289 426 183 319 3.98e-15 77.0
MS.gene060503.t1 MTR_4g066460 28.313 166 103 3 262 426 138 288 4.33e-15 76.6
MS.gene060503.t1 MTR_2g081040 35.032 157 87 4 286 430 174 327 7.33e-15 76.3
MS.gene060503.t1 MTR_0011s0210 30.216 139 94 2 289 426 183 319 8.71e-15 75.9
MS.gene060503.t1 MTR_4g067010 26.857 175 107 5 258 426 121 280 9.13e-15 75.5
MS.gene060503.t1 MTR_4g097920 28.643 199 115 7 243 428 102 286 1.24e-14 75.1
MS.gene060503.t1 MTR_4g097920 28.643 199 115 7 243 428 87 271 1.27e-14 75.1
MS.gene060503.t1 MTR_3g030700 31.792 173 108 4 266 431 173 342 1.42e-14 75.5
MS.gene060503.t1 MTR_8g098275 39.806 103 52 2 288 380 307 409 2.97e-14 75.1
MS.gene060503.t1 MTR_8g098275 39.806 103 52 2 288 380 243 345 3.03e-14 75.1
MS.gene060503.t1 MTR_8g098275 39.806 103 52 2 288 380 400 502 3.22e-14 75.5
MS.gene060503.t1 MTR_4g066380 26.214 206 130 6 263 462 137 326 4.24e-14 73.6
MS.gene060503.t1 MTR_7g083900 60.000 55 22 0 287 341 284 338 7.97e-14 73.9
MS.gene060503.t1 MTR_3g498695 58.182 55 23 0 287 341 296 350 8.02e-14 73.9
MS.gene060503.t1 MTR_3g498695 58.182 55 23 0 287 341 296 350 8.37e-14 73.6
MS.gene060503.t1 MTR_7g083900 60.000 55 22 0 287 341 284 338 8.61e-14 73.6
MS.gene060503.t1 MTR_6g047550 37.838 111 61 2 260 370 148 250 1.47e-13 71.2
MS.gene060503.t1 MTR_2g010450 30.345 145 85 3 289 425 177 313 1.84e-13 72.0
MS.gene060503.t1 MTR_4g097950 30.714 140 87 3 285 418 67 202 4.04e-13 69.7
MS.gene060503.t1 MTR_2g038040 53.226 62 22 1 290 344 53 114 4.74e-13 69.3
MS.gene060503.t1 MTR_0246s0020 49.254 67 30 1 289 355 198 260 7.05e-13 70.1
MS.gene060503.t1 MTR_0246s0020 49.254 67 30 1 289 355 198 260 7.91e-13 70.1
MS.gene060503.t1 MTR_2g104530 28.467 137 93 1 290 426 106 237 1.19e-12 68.6
MS.gene060503.t1 MTR_8g009020 25.316 158 112 2 275 426 129 286 2.16e-12 68.6
MS.gene060503.t1 MTR_4g094762 29.012 162 84 5 290 431 293 443 5.75e-12 68.2
MS.gene060503.t1 MTR_4g057270 30.000 160 71 7 293 431 153 292 3.83e-11 64.7
MS.gene060503.t1 MTR_2g104500 25.269 186 131 5 241 426 58 235 4.84e-11 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene060503.t1 AT4G00870 37.527 461 200 14 20 460 29 421 2.53e-73 237
MS.gene060503.t1 AT4G17880 29.050 537 269 13 26 462 63 587 2.51e-69 231
MS.gene060503.t1 AT5G46830 33.143 525 237 18 18 460 18 510 7.29e-65 218
MS.gene060503.t1 AT1G32640 51.980 202 89 4 263 461 427 623 4.37e-57 199
MS.gene060503.t1 AT1G32640 31.053 190 94 3 25 181 67 252 1.06e-29 122
MS.gene060503.t1 AT5G46760 44.351 239 121 5 229 462 359 590 8.47e-54 190
MS.gene060503.t1 AT5G46760 32.065 184 89 5 25 176 51 230 1.56e-26 113
MS.gene060503.t1 AT4G16430 27.521 476 258 16 21 462 45 467 1.05e-40 152
MS.gene060503.t1 AT2G46510 40.860 186 88 4 269 452 376 541 2.50e-34 135
MS.gene060503.t1 AT1G01260 38.889 180 85 3 273 452 417 571 1.21e-30 125
MS.gene060503.t1 AT1G01260 28.061 196 100 5 26 184 48 239 3.13e-13 72.4
MS.gene060503.t1 AT1G01260 38.889 180 85 3 273 452 417 571 1.21e-30 125
MS.gene060503.t1 AT1G01260 28.061 196 100 5 26 184 48 239 3.13e-13 72.4
MS.gene060503.t1 AT1G01260 38.889 180 85 3 273 452 417 571 1.21e-30 125
MS.gene060503.t1 AT1G01260 28.061 196 100 5 26 184 48 239 3.13e-13 72.4
MS.gene060503.t1 AT4G00480 22.088 498 281 15 31 441 26 503 1.97e-18 88.2
MS.gene060503.t1 AT4G00480 22.245 490 274 15 31 433 26 495 4.16e-18 87.4
MS.gene060503.t1 AT4G00480 21.429 518 300 15 31 461 26 523 5.63e-18 87.0
MS.gene060503.t1 AT4G37850 31.325 166 104 5 266 426 130 290 2.15e-16 80.5
MS.gene060503.t1 AT4G37850 31.325 166 104 5 266 426 130 290 2.15e-16 80.5
MS.gene060503.t1 AT1G63650 27.711 166 103 3 289 437 405 570 6.87e-16 80.5
MS.gene060503.t1 AT1G63650 27.711 166 103 3 289 437 405 570 6.87e-16 80.5
MS.gene060503.t1 AT1G63650 27.711 166 103 3 289 437 405 570 6.87e-16 80.5
MS.gene060503.t1 AT4G09820 32.824 131 65 2 288 417 362 470 1.06e-14 76.6
MS.gene060503.t1 AT1G12860 57.895 57 24 0 287 343 265 321 1.36e-14 75.9
MS.gene060503.t1 AT3G26744 60.000 55 22 0 287 341 305 359 1.39e-14 76.3
MS.gene060503.t1 AT3G26744 60.000 55 22 0 287 341 305 359 1.42e-14 76.3
MS.gene060503.t1 AT3G26744 60.000 55 22 0 287 341 305 359 1.42e-14 76.3
MS.gene060503.t1 AT3G26744 60.000 55 22 0 287 341 305 359 1.42e-14 76.3
MS.gene060503.t1 AT1G12860 57.895 57 24 0 287 343 265 321 1.70e-14 75.9
MS.gene060503.t1 AT3G06120 28.415 183 110 4 288 449 1 183 5.16e-14 71.2
MS.gene060503.t1 AT5G57150 26.908 249 138 10 233 461 7 231 7.54e-14 71.6
MS.gene060503.t1 AT5G57150 27.193 228 128 8 233 441 7 215 1.57e-13 70.5
MS.gene060503.t1 AT5G57150 27.273 231 129 9 233 443 7 218 1.60e-13 70.9
MS.gene060503.t1 AT4G29930 54.098 61 21 1 290 343 55 115 2.21e-13 70.1
MS.gene060503.t1 AT5G57150 26.754 228 129 8 233 441 7 215 2.49e-13 69.7
MS.gene060503.t1 AT4G29930 54.098 61 21 1 290 343 55 115 2.57e-13 69.7
MS.gene060503.t1 AT5G57150 36.719 128 59 5 233 359 7 113 2.62e-13 69.7
MS.gene060503.t1 AT4G29930 54.098 61 21 1 290 343 55 115 2.78e-13 69.7
MS.gene060503.t1 AT4G29930 55.000 60 20 1 291 343 56 115 4.03e-13 68.2
MS.gene060503.t1 AT4G29930 54.098 61 21 1 290 343 55 115 4.21e-13 69.7
MS.gene060503.t1 AT4G29930 54.098 61 21 1 290 343 55 115 4.21e-13 69.7
MS.gene060503.t1 AT4G29930 54.098 61 21 1 290 343 55 115 5.29e-13 69.3
MS.gene060503.t1 AT1G10610 31.579 152 97 4 275 421 220 369 1.66e-12 69.3
MS.gene060503.t1 AT1G10610 32.847 137 85 4 290 421 265 399 1.98e-12 69.3
MS.gene060503.t1 AT1G10610 32.847 137 85 4 290 421 168 302 2.09e-12 68.6
MS.gene060503.t1 AT5G41315 32.258 93 49 1 289 381 441 519 2.75e-12 69.3
MS.gene060503.t1 AT5G41315 32.258 93 49 1 289 381 446 524 2.83e-12 69.3
MS.gene060503.t1 AT5G41315 32.258 93 49 1 289 381 449 527 3.00e-12 69.3
MS.gene060503.t1 AT2G22760 28.415 183 106 7 276 439 100 276 5.84e-12 66.6
MS.gene060503.t1 AT3G24140 24.186 215 136 4 269 456 175 389 6.02e-12 67.8
MS.gene060503.t1 AT3G24140 24.186 215 136 4 269 456 175 389 7.51e-12 67.4
MS.gene060503.t1 AT2G16910 54.000 50 23 0 293 342 318 367 9.75e-12 67.4
MS.gene060503.t1 AT2G22760 29.697 165 96 6 276 426 100 258 2.02e-11 65.1
MS.gene060503.t1 AT4G38070 38.095 84 49 1 258 341 67 147 9.88e-11 62.8

Find 76 sgRNAs with CRISPR-Local

Find 94 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTGAAGGATTTGGAGTTTC+AGG 0.119847 8.1:+75631306 MS.gene060503:CDS
TATTCATTTGTTGCAAATTT+TGG 0.152440 8.1:-75630754 None:intergenic
TCCAAACCCAATCCGAAAAT+TGG 0.161848 8.1:+75630168 MS.gene060503:CDS
GAGATTGATGTGAAGATAAT+AGG 0.216324 8.1:+75631217 MS.gene060503:CDS
GTTTGAAATTGTTGTTTCTT+TGG 0.232211 8.1:-75630273 None:intergenic
AAGGTAAACCTTTCTTATCT+TGG 0.238310 8.1:+75630228 MS.gene060503:CDS
ATGACAATATCCAACAAAAT+TGG 0.270722 8.1:+75630558 MS.gene060503:CDS
TTATCCATCTTTGAAACATT+TGG 0.290544 8.1:-75631004 None:intergenic
AGGTAAACCTTTCTTATCTT+GGG 0.292720 8.1:+75630229 MS.gene060503:CDS
AGAGGAAGAAAACCGTTATC+AGG 0.296834 8.1:+75630893 MS.gene060503:CDS
ATCTTAAAGCATAGAATCTA+TGG 0.304209 8.1:-75630973 None:intergenic
AAGATTGAAGATTTAGAATC+AGG 0.311502 8.1:+75631076 MS.gene060503:CDS
ATTCTTGTTGGTGTTGCTAA+TGG 0.335278 8.1:-75630833 None:intergenic
TACCGATGTCGGCGAATGTT+TGG 0.341437 8.1:-75630682 None:intergenic
AATGAGTGTCTTGAAGGATT+TGG 0.342944 8.1:+75631297 MS.gene060503:CDS
TCCATGCACGTGTGCTTCAT+TGG 0.351119 8.1:-75630480 None:intergenic
AGGGTTAGGGTTTGTGGTGA+AGG 0.352816 8.1:-75630122 None:intergenic
TGTTGAAGGGTTAGGGTTTG+TGG 0.360355 8.1:-75630128 None:intergenic
AACTAACGGTGTAATCGAAA+TGG 0.368563 8.1:+75630529 MS.gene060503:CDS
TAGATTTAGCTTGGTGTACT+AGG 0.375629 8.1:-75630583 None:intergenic
ATCAGTGTCTTCTCCGGAAC+CGG 0.379047 8.1:-75630711 None:intergenic
TCAGCCATTCTCATGAAATT+AGG 0.386390 8.1:+75631427 MS.gene060503:CDS
TTGTTGTTTCTTTGGTTCCT+TGG 0.388661 8.1:-75630265 None:intergenic
ATCGACTGTTAATGAAGTTA+TGG 0.398979 8.1:+75631345 MS.gene060503:CDS
CAAACAACAAAAGATGAAAA+AGG 0.404302 8.1:+75630209 MS.gene060503:CDS
ATCGGTATCATCTCCGGTTC+CGG 0.412266 8.1:+75630698 MS.gene060503:CDS
AAGATTAATGAGTGTCTTGA+AGG 0.413301 8.1:+75631291 MS.gene060503:CDS
GGGTTAGGGTTTGTGGTGAA+GGG 0.421882 8.1:-75630121 None:intergenic
TTGCTTTAGATTCTGTACTT+TGG 0.425852 8.1:+75630414 MS.gene060503:CDS
AGATTTGTCAAGTGGGTTTG+TGG 0.429194 8.1:-75630654 None:intergenic
GGTTCCAAATGTTTCAAAGA+TGG 0.435449 8.1:+75631000 MS.gene060503:CDS
AAAAGTGAAGATGGAAATGA+TGG 0.445716 8.1:+75631117 MS.gene060503:CDS
CCTTTCTTATCTTGGGGTGA+AGG 0.446476 8.1:+75630236 MS.gene060503:CDS
TCTTCCGTGCTCATCTCGTT+AGG 0.461472 8.1:-75631391 None:intergenic
CCAAACCCAATCCGAAAATT+GGG 0.468035 8.1:+75630169 MS.gene060503:CDS
TGTCTCATCAGTGTCTTCTC+CGG 0.468594 8.1:-75630717 None:intergenic
GACAGCAACACGTGTACAAA+TGG 0.479858 8.1:+75630305 MS.gene060503:CDS
CTGACCTAATTTCATGAGAA+TGG 0.482002 8.1:-75631431 None:intergenic
GCTTCTTCAATTTCATTACC+TGG 0.483921 8.1:+75630383 MS.gene060503:CDS
ACTAACGGTGTAATCGAAAT+GGG 0.485666 8.1:+75630530 MS.gene060503:CDS
TTTGAAACATAGATTTAGCT+TGG 0.486812 8.1:-75630592 None:intergenic
TCTGCTTCCACATGGTTCAT+CGG 0.487476 8.1:-75630926 None:intergenic
CCTTCACCCCAAGATAAGAA+AGG 0.497048 8.1:-75630236 None:intergenic
TCGATTACACCGTTAGTTGT+CGG 0.498052 8.1:-75630524 None:intergenic
ATTTGTATTCCGACAACTAA+CGG 0.507792 8.1:+75630515 MS.gene060503:CDS
GGTGTTTGAGTCCCTGATAA+CGG 0.510477 8.1:-75630905 None:intergenic
TTTAGAATCAGGGCAACAAA+AGG 0.514365 8.1:+75631087 MS.gene060503:CDS
TTTGTCTCTCTGCTTCCACA+TGG 0.518910 8.1:-75630934 None:intergenic
TCCAATGAAGCACACGTGCA+TGG 0.524272 8.1:+75630479 MS.gene060503:CDS
TGGAACATAGATTTGTCAAG+TGG 0.527748 8.1:-75630662 None:intergenic
ATTCTATGCTTTAAGATCTG+TGG 0.527981 8.1:+75630979 MS.gene060503:CDS
GCCGACATCGGTATCATCTC+CGG 0.528009 8.1:+75630692 MS.gene060503:CDS
GATAATAGGAAATGATGCTA+TGG 0.531125 8.1:+75631231 MS.gene060503:CDS
ACACTCATTAATCTTGCACT+AGG 0.531898 8.1:-75631283 None:intergenic
AATTGAAGAAGCATTACCTA+TGG 0.532558 8.1:-75630372 None:intergenic
GGTAAACCTTTCTTATCTTG+GGG 0.537701 8.1:+75630230 MS.gene060503:CDS
TTCCAAACATTCGCCGACAT+CGG 0.537863 8.1:+75630680 MS.gene060503:CDS
ATGTGGAAGCAGAGAGACAA+AGG 0.547229 8.1:+75630936 MS.gene060503:CDS
ATCATAGAGGAAGATGAAGA+AGG 0.553296 8.1:-75630074 None:intergenic
TCAAAAGCATTAACATTACA+AGG 0.560113 8.1:-75631196 None:intergenic
AGATTGAAGATTTAGAATCA+GGG 0.563283 8.1:+75631077 MS.gene060503:CDS
GAGGAAGAAAACCGTTATCA+GGG 0.566872 8.1:+75630894 MS.gene060503:CDS
CTTGTTGGTGTTGCTAATGG+TGG 0.567083 8.1:-75630830 None:intergenic
TGCAACGGCAACTTCAACTG+TGG 0.578390 8.1:+75631159 MS.gene060503:CDS
TGGGGTGAAGGCTATTTCCA+AGG 0.580662 8.1:+75630248 MS.gene060503:CDS
GGAACATAGATTTGTCAAGT+GGG 0.591339 8.1:-75630661 None:intergenic
GTACAAATGGTGATGCTGAA+TGG 0.602234 8.1:+75630318 MS.gene060503:CDS
TCTAAAGCAAAAGCTTTACC+AGG 0.602957 8.1:-75630401 None:intergenic
AAAAGAGAACCTAAGAAGAG+AGG 0.611347 8.1:+75630875 MS.gene060503:CDS
TGTCGATAATAACAGTGCAA+CGG 0.631393 8.1:+75631144 MS.gene060503:CDS
ATGAGGAAATGATATCATAG+AGG 0.640466 8.1:-75630087 None:intergenic
CACTCATTAATCTTGCACTA+GGG 0.642950 8.1:-75631282 None:intergenic
ACCGGAGATGATACCGATGT+CGG 0.648663 8.1:-75630693 None:intergenic
AGTTCCTAACGAGATGAGCA+CGG 0.664680 8.1:+75631387 MS.gene060503:CDS
TTCTGAGTTAGATTCTGACG+AGG 0.697473 8.1:-75630618 None:intergenic
TCAAACACCGATGAACCATG+TGG 0.729846 8.1:+75630919 MS.gene060503:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAAATTGAAGTTTTTGTTGA+AGG - chr8.1:75630145-75630164 None:intergenic 20.0%
!!! AAATTGAAGTTTTTGTTGAA+GGG - chr8.1:75630144-75630163 None:intergenic 20.0%
!!! TATTCATTTGTTGCAAATTT+TGG - chr8.1:75630757-75630776 None:intergenic 20.0%
! ATCTTAAAGCATAGAATCTA+TGG - chr8.1:75630976-75630995 None:intergenic 25.0%
! ATGACAATATCCAACAAAAT+TGG + chr8.1:75630558-75630577 MS.gene060503:CDS 25.0%
! CAAACAACAAAAGATGAAAA+AGG + chr8.1:75630209-75630228 MS.gene060503:CDS 25.0%
!! AAGATTGAAGATTTAGAATC+AGG + chr8.1:75631076-75631095 MS.gene060503:CDS 25.0%
!! AGATTGAAGATTTAGAATCA+GGG + chr8.1:75631077-75631096 MS.gene060503:CDS 25.0%
!! TTTGAAACATAGATTTAGCT+TGG - chr8.1:75630595-75630614 None:intergenic 25.0%
!!! GAATCTTTTTCAATTCTTGT+TGG - chr8.1:75630848-75630867 None:intergenic 25.0%
!!! GTTTGAAATTGTTGTTTCTT+TGG - chr8.1:75630276-75630295 None:intergenic 25.0%
!!! TAACATTACAAGGTCTTTTT+TGG - chr8.1:75631189-75631208 None:intergenic 25.0%
!!! TCAAAAGCATTAACATTACA+AGG - chr8.1:75631199-75631218 None:intergenic 25.0%
!!! TTATCCATCTTTGAAACATT+TGG - chr8.1:75631007-75631026 None:intergenic 25.0%
AAAAGTGAAGATGGAAATGA+TGG + chr8.1:75631117-75631136 MS.gene060503:CDS 30.0%
AAGATTAATGAGTGTCTTGA+AGG + chr8.1:75631291-75631310 MS.gene060503:CDS 30.0%
AAGGTAAACCTTTCTTATCT+TGG + chr8.1:75630228-75630247 MS.gene060503:CDS 30.0%
AGGTAAACCTTTCTTATCTT+GGG + chr8.1:75630229-75630248 MS.gene060503:CDS 30.0%
ATCGACTGTTAATGAAGTTA+TGG + chr8.1:75631345-75631364 MS.gene060503:CDS 30.0%
ATGAGGAAATGATATCATAG+AGG - chr8.1:75630090-75630109 None:intergenic 30.0%
ATTTGTATTCCGACAACTAA+CGG + chr8.1:75630515-75630534 MS.gene060503:CDS 30.0%
GAGATTGATGTGAAGATAAT+AGG + chr8.1:75631217-75631236 MS.gene060503:CDS 30.0%
GATAATAGGAAATGATGCTA+TGG + chr8.1:75631231-75631250 MS.gene060503:CDS 30.0%
! AAGTTTTTGTTGAAGGGTTA+GGG - chr8.1:75630138-75630157 None:intergenic 30.0%
! TTGAGTCGAAATTTTTCCAT+AGG + chr8.1:75630356-75630375 MS.gene060503:CDS 30.0%
! TTGCTTTAGATTCTGTACTT+TGG + chr8.1:75630414-75630433 MS.gene060503:CDS 30.0%
!! AATTGAAGAAGCATTACCTA+TGG - chr8.1:75630375-75630394 None:intergenic 30.0%
!! ATTCTATGCTTTAAGATCTG+TGG + chr8.1:75630979-75630998 MS.gene060503:CDS 30.0%
!!! TTTTGTTGTTTGCCACAAAA+TGG - chr8.1:75630201-75630220 None:intergenic 30.0%
AAAAGAGAACCTAAGAAGAG+AGG + chr8.1:75630875-75630894 MS.gene060503:CDS 35.0%
AACTAACGGTGTAATCGAAA+TGG + chr8.1:75630529-75630548 MS.gene060503:CDS 35.0%
AATGAGTGTCTTGAAGGATT+TGG + chr8.1:75631297-75631316 MS.gene060503:CDS 35.0%
ACACTCATTAATCTTGCACT+AGG - chr8.1:75631286-75631305 None:intergenic 35.0%
ACTAACGGTGTAATCGAAAT+GGG + chr8.1:75630530-75630549 MS.gene060503:CDS 35.0%
ATCATAGAGGAAGATGAAGA+AGG - chr8.1:75630077-75630096 None:intergenic 35.0%
CACTCATTAATCTTGCACTA+GGG - chr8.1:75631285-75631304 None:intergenic 35.0%
CTGACCTAATTTCATGAGAA+TGG - chr8.1:75631434-75631453 None:intergenic 35.0%
GCTTCTTCAATTTCATTACC+TGG + chr8.1:75630383-75630402 MS.gene060503:CDS 35.0%
GGAACATAGATTTGTCAAGT+GGG - chr8.1:75630664-75630683 None:intergenic 35.0%
GGAATCGAAAAAAGTGAAGA+TGG + chr8.1:75631108-75631127 MS.gene060503:CDS 35.0%
GGTAAACCTTTCTTATCTTG+GGG + chr8.1:75630230-75630249 MS.gene060503:CDS 35.0%
GGTTCCAAATGTTTCAAAGA+TGG + chr8.1:75631000-75631019 MS.gene060503:CDS 35.0%
TCAGCCATTCTCATGAAATT+AGG + chr8.1:75631427-75631446 MS.gene060503:CDS 35.0%
TGGAACATAGATTTGTCAAG+TGG - chr8.1:75630665-75630684 None:intergenic 35.0%
TGTCGATAATAACAGTGCAA+CGG + chr8.1:75631144-75631163 MS.gene060503:CDS 35.0%
! AATGGCATAGACCCAATTTT+CGG - chr8.1:75630183-75630202 None:intergenic 35.0%
! ATAGACCCAATTTTCGGATT+GGG - chr8.1:75630177-75630196 None:intergenic 35.0%
! GAAGGGTTTTCATTTTCATG+AGG - chr8.1:75630107-75630126 None:intergenic 35.0%
! GAAGTTTTTGTTGAAGGGTT+AGG - chr8.1:75630139-75630158 None:intergenic 35.0%
! GTACTAGGTTCCAATTTTGT+TGG - chr8.1:75630571-75630590 None:intergenic 35.0%
! TCTAAAGCAAAAGCTTTACC+AGG - chr8.1:75630404-75630423 None:intergenic 35.0%
! TTCTCTTTTATCAGATGCAG+TGG + chr8.1:75631030-75631049 MS.gene060503:CDS 35.0%
! TTTAGAATCAGGGCAACAAA+AGG + chr8.1:75631087-75631106 MS.gene060503:CDS 35.0%
!! ATTCTTGTTGGTGTTGCTAA+TGG - chr8.1:75630836-75630855 None:intergenic 35.0%
!! TAGATTTAGCTTGGTGTACT+AGG - chr8.1:75630586-75630605 None:intergenic 35.0%
!! TTGTTGTTTCTTTGGTTCCT+TGG - chr8.1:75630268-75630287 None:intergenic 35.0%
AGAGGAAGAAAACCGTTATC+AGG + chr8.1:75630893-75630912 MS.gene060503:CDS 40.0%
CCAAACCCAATCCGAAAATT+GGG + chr8.1:75630169-75630188 MS.gene060503:CDS 40.0%
GAGGAAGAAAACCGTTATCA+GGG + chr8.1:75630894-75630913 MS.gene060503:CDS 40.0%
TCCAAACCCAATCCGAAAAT+TGG + chr8.1:75630168-75630187 MS.gene060503:CDS 40.0%
TCGATTACACCGTTAGTTGT+CGG - chr8.1:75630527-75630546 None:intergenic 40.0%
TTCTGAGTTAGATTCTGACG+AGG - chr8.1:75630621-75630640 None:intergenic 40.0%
! AGATTTGTCAAGTGGGTTTG+TGG - chr8.1:75630657-75630676 None:intergenic 40.0%
! CATAGACCCAATTTTCGGAT+TGG - chr8.1:75630178-75630197 None:intergenic 40.0%
! CTTGAAGGATTTGGAGTTTC+AGG + chr8.1:75631306-75631325 MS.gene060503:CDS 40.0%
! GGTTTTCTTCCTCTCTTCTT+AGG - chr8.1:75630887-75630906 None:intergenic 40.0%
! GTACAAATGGTGATGCTGAA+TGG + chr8.1:75630318-75630337 MS.gene060503:CDS 40.0%
!!! ATTGGGTCTATGCCATTTTG+TGG + chr8.1:75630186-75630205 MS.gene060503:CDS 40.0%
AGTTCCTAACGAGATGAGCA+CGG + chr8.1:75631387-75631406 MS.gene060503:CDS 45.0%
ATGTGGAAGCAGAGAGACAA+AGG + chr8.1:75630936-75630955 MS.gene060503:CDS 45.0%
CCTTCACCCCAAGATAAGAA+AGG - chr8.1:75630239-75630258 None:intergenic 45.0%
CCTTTCTTATCTTGGGGTGA+AGG + chr8.1:75630236-75630255 MS.gene060503:CDS 45.0%
GACAGCAACACGTGTACAAA+TGG + chr8.1:75630305-75630324 MS.gene060503:CDS 45.0%
GGTGTTTGAGTCCCTGATAA+CGG - chr8.1:75630908-75630927 None:intergenic 45.0%
TCAAACACCGATGAACCATG+TGG + chr8.1:75630919-75630938 MS.gene060503:CDS 45.0%
TCTGCTTCCACATGGTTCAT+CGG - chr8.1:75630929-75630948 None:intergenic 45.0%
TGTCTCATCAGTGTCTTCTC+CGG - chr8.1:75630720-75630739 None:intergenic 45.0%
TGTTGAAGGGTTAGGGTTTG+TGG - chr8.1:75630131-75630150 None:intergenic 45.0%
TTCCAAACATTCGCCGACAT+CGG + chr8.1:75630680-75630699 MS.gene060503:CDS 45.0%
TTTGTCTCTCTGCTTCCACA+TGG - chr8.1:75630937-75630956 None:intergenic 45.0%
!! CCCAATTTTCGGATTGGGTT+TGG - chr8.1:75630172-75630191 None:intergenic 45.0%
!! CTTGTTGGTGTTGCTAATGG+TGG - chr8.1:75630833-75630852 None:intergenic 45.0%
ACCGGAGATGATACCGATGT+CGG - chr8.1:75630696-75630715 None:intergenic 50.0%
AGGGTTAGGGTTTGTGGTGA+AGG - chr8.1:75630125-75630144 None:intergenic 50.0%
ATCAGTGTCTTCTCCGGAAC+CGG - chr8.1:75630714-75630733 None:intergenic 50.0%
ATCGGTATCATCTCCGGTTC+CGG + chr8.1:75630698-75630717 MS.gene060503:CDS 50.0%
GGGTTAGGGTTTGTGGTGAA+GGG - chr8.1:75630124-75630143 None:intergenic 50.0%
TACCGATGTCGGCGAATGTT+TGG - chr8.1:75630685-75630704 None:intergenic 50.0%
TCCAATGAAGCACACGTGCA+TGG + chr8.1:75630479-75630498 MS.gene060503:CDS 50.0%
TCCATGCACGTGTGCTTCAT+TGG - chr8.1:75630483-75630502 None:intergenic 50.0%
TCTTCCGTGCTCATCTCGTT+AGG - chr8.1:75631394-75631413 None:intergenic 50.0%
TGCAACGGCAACTTCAACTG+TGG + chr8.1:75631159-75631178 MS.gene060503:CDS 50.0%
! TGGGGTGAAGGCTATTTCCA+AGG + chr8.1:75630248-75630267 MS.gene060503:CDS 50.0%
GCCGACATCGGTATCATCTC+CGG + chr8.1:75630692-75630711 MS.gene060503:CDS 55.0%


Chromosome Type Strat End Strand Name
chr8.1 gene 75630068 75631456 75630068 ID=MS.gene060503
chr8.1 mRNA 75630068 75631456 75630068 ID=MS.gene060503.t1;Parent=MS.gene060503
chr8.1 exon 75630068 75631456 75630068 ID=MS.gene060503.t1.exon1;Parent=MS.gene060503.t1
chr8.1 CDS 75630068 75631456 75630068 ID=cds.MS.gene060503.t1;Parent=MS.gene060503.t1
Gene Sequence

>MS.gene060503

ATGTCACCTTCTTCATCTTCCTCTATGATATCATTTCCTCATGAAAATGAAAACCCTTCACCACAAACCCTAACCCTTCAACAAAAACTTCAATTTTTAGTCCAAACCCAATCCGAAAATTGGGTCTATGCCATTTTGTGGCAAACAACAAAAGATGAAAAAGGTAAACCTTTCTTATCTTGGGGTGAAGGCTATTTCCAAGGAACCAAAGAAACAACAATTTCAAACAAACAACACGACAGCAACACGTGTACAAATGGTGATGCTGAATGGTTCTATGTGATGTCGTTGAGTCGAAATTTTTCCATAGGTAATGCTTCTTCAATTTCATTACCTGGTAAAGCTTTTGCTTTAGATTCTGTACTTTGGTTGAATAGTAAACATGAACTTCAAATCTATAACTGTGAGAGATCCAATGAAGCACACGTGCATGGAATTGAAACATTGATTTGTATTCCGACAACTAACGGTGTAATCGAAATGGGTTCTTATGACAATATCCAACAAAATTGGAACCTAGTACACCAAGCTAAATCTATGTTTCAAACATCCTCGTCAGAATCTAACTCAGAATTAGATCTTATCTCCACAAACCCACTTGACAAATCTATGTTCCAAACATTCGCCGACATCGGTATCATCTCCGGTTCCGGAGAAGACACTGATGAGACACAAAAAACAATGAACCAAAATTTGCAACAAATGAATAACATTGTTTCATCATGTTCTTTTGATTCTGAAAATTCTGATTCAGAATATTGTCCACCATTAGCAACACCAACAAGAATTGAAAAAGATTCTTTTGAGAAAAGAGAACCTAAGAAGAGAGGAAGAAAACCGTTATCAGGGACTCAAACACCGATGAACCATGTGGAAGCAGAGAGACAAAGGAGAGAGAAACTAAACCATAGATTCTATGCTTTAAGATCTGTGGTTCCAAATGTTTCAAAGATGGATAAAGCTTCTCTTTTATCAGATGCAGTGGATTACATCAACGAGTTAAAAGCAAAGATTGAAGATTTAGAATCAGGGCAACAAAAGGAATCGAAAAAAGTGAAGATGGAAATGATGGAAATTGTCGATAATAACAGTGCAACGGCAACTTCAACTGTGGTAGACCAAAAAAGACCTTGTAATGTTAATGCTTTTGAGATTGATGTGAAGATAATAGGAAATGATGCTATGGTAAGAGTTCAAAGTGAGAATGTTAATCACCCTAGTGCAAGATTAATGAGTGTCTTGAAGGATTTGGAGTTTCAGGTACATCATGCTAGTATATCGACTGTTAATGAAGTTATGGTTCAAGATGTTGTTGTTCGAGTTCCTAACGAGATGAGCACGGAAGAGAGTCTAATTAGATCAGCCATTCTCATGAAATTAGGTCAGTAG
Protein sequence

>MS.gene060503.t1

MSPSSSSSMISFPHENENPSPQTLTLQQKLQFLVQTQSENWVYAILWQTTKDEKGKPFLSWGEGYFQGTKETTISNKQHDSNTCTNGDAEWFYVMSLSRNFSIGNASSISLPGKAFALDSVLWLNSKHELQIYNCERSNEAHVHGIETLICIPTTNGVIEMGSYDNIQQNWNLVHQAKSMFQTSSSESNSELDLISTNPLDKSMFQTFADIGIISGSGEDTDETQKTMNQNLQQMNNIVSSCSFDSENSDSEYCPPLATPTRIEKDSFEKREPKKRGRKPLSGTQTPMNHVEAERQRREKLNHRFYALRSVVPNVSKMDKASLLSDAVDYINELKAKIEDLESGQQKESKKVKMEMMEIVDNNSATATSTVVDQKRPCNVNAFEIDVKIIGNDAMVRVQSENVNHPSARLMSVLKDLEFQVHHASISTVNEVMVQDVVVRVPNEMSTEESLIRSAILMKLGQ