Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene067337.t1 | GAU33600.1 | 96.3 | 81 | 3 | 0 | 1 | 81 | 329 | 409 | 5.70E-35 | 156.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene067337.t1 | Q9ZT63 | 82.7 | 81 | 14 | 0 | 1 | 81 | 311 | 391 | 2.3e-32 | 139.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene067337.t1 | A0A2K3P9K7 | 96.3 | 81 | 3 | 0 | 1 | 81 | 321 | 401 | 4.1e-35 | 156.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene058906 | MS.gene067337 | 0.800981 | 1.12E-48 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene067337.t1 | MTR_8g024240 | 95.062 | 81 | 4 | 0 | 1 | 81 | 298 | 378 | 3.19e-49 | 159 |
| MS.gene067337.t1 | MTR_2g036390 | 83.951 | 81 | 13 | 0 | 1 | 81 | 320 | 400 | 8.40e-43 | 143 |
| MS.gene067337.t1 | MTR_4g064893 | 45.679 | 81 | 44 | 0 | 1 | 81 | 308 | 388 | 6.92e-18 | 76.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene067337.t1 | AT2G46800 | 82.716 | 81 | 14 | 0 | 1 | 81 | 311 | 391 | 1.37e-42 | 142 |
| MS.gene067337.t1 | AT2G46800 | 82.716 | 81 | 14 | 0 | 1 | 81 | 311 | 391 | 1.37e-42 | 142 |
| MS.gene067337.t1 | AT2G46800 | 82.716 | 81 | 14 | 0 | 1 | 81 | 311 | 391 | 1.37e-42 | 142 |
| MS.gene067337.t1 | AT2G46800 | 82.716 | 81 | 14 | 0 | 1 | 81 | 311 | 391 | 1.37e-42 | 142 |
| MS.gene067337.t1 | AT2G46800 | 82.716 | 81 | 14 | 0 | 1 | 81 | 311 | 391 | 1.37e-42 | 142 |
| MS.gene067337.t1 | AT3G58810 | 80.247 | 81 | 16 | 0 | 1 | 81 | 305 | 385 | 1.17e-40 | 137 |
| MS.gene067337.t1 | AT3G58810 | 80.247 | 81 | 16 | 0 | 1 | 81 | 344 | 424 | 1.47e-40 | 137 |
| MS.gene067337.t1 | AT3G61940 | 74.074 | 81 | 21 | 0 | 1 | 81 | 247 | 327 | 1.92e-36 | 125 |
| MS.gene067337.t1 | AT2G29410 | 38.272 | 81 | 50 | 0 | 1 | 81 | 287 | 367 | 6.65e-17 | 73.6 |
| MS.gene067337.t1 | AT1G33020 | 63.636 | 44 | 16 | 0 | 32 | 75 | 492 | 535 | 1.48e-13 | 64.3 |
Find 18 sgRNAs with CRISPR-Local
Find 22 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAATATGCTGCGAAACATTT+TGG | 0.138374 | 8.4:+70812860 | None:intergenic |
| CTGTTCATGAGCTCCACATT+TGG | 0.312952 | 8.4:+70812964 | MS.gene067337:CDS |
| TGTTCATGAGCTCCACATTT+GGG | 0.346809 | 8.4:+70812965 | MS.gene067337:CDS |
| CCGCGATGCCGATGCAGAAA+TGG | 0.388971 | 8.4:+70813034 | MS.gene067337:CDS |
| CACATTTGGGCCATTACAGT+TGG | 0.449190 | 8.4:+70812978 | MS.gene067337:CDS |
| TGATGCTACTCAACTTGAAA+GGG | 0.461546 | 8.4:+70812914 | MS.gene067337:CDS |
| CAAAATCTTCCCAACTGTAA+TGG | 0.466674 | 8.4:-70812988 | None:intergenic |
| TTGATGCTACTCAACTTGAA+AGG | 0.524765 | 8.4:+70812913 | MS.gene067337:CDS |
| GTCCAACACCATTTCTGCAT+CGG | 0.525223 | 8.4:-70813042 | None:intergenic |
| ACATTTGGGCCATTACAGTT+GGG | 0.528212 | 8.4:+70812979 | MS.gene067337:CDS |
| ATTGGAGATGGAGGAAGTAG+TGG | 0.552789 | 8.4:+70812941 | MS.gene067337:CDS |
| TGCCGATGCAGAAATGGTGT+TGG | 0.560331 | 8.4:+70813040 | MS.gene067337:CDS |
| CAACTGTAATGGCCCAAATG+TGG | 0.594141 | 8.4:-70812977 | None:intergenic |
| TGCAGAAATGGTGTTGGACA+AGG | 0.626604 | 8.4:+70813046 | MS.gene067337:CDS |
| GATGCTACTCAACTTGAAAG+GGG | 0.649578 | 8.4:+70812915 | MS.gene067337:CDS |
| TGAGTAGCATCAATCTCTCG+AGG | 0.673866 | 8.4:-70812903 | None:intergenic |
| ATGCTACTCAACTTGAAAGG+GGG | 0.675065 | 8.4:+70812916 | MS.gene067337:CDS |
| CCATTTCTGCATCGGCATCG+CGG | 0.700876 | 8.4:-70813034 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAACATTTTGGAAGTTCTGA+TGG | + | chr8.4:70812872-70812891 | MS.gene067337:CDS | 30.0% |
| CAAAATCTTCCCAACTGTAA+TGG | - | chr8.4:70812991-70813010 | None:intergenic | 35.0% | |
| TGATGCTACTCAACTTGAAA+GGG | + | chr8.4:70812914-70812933 | MS.gene067337:CDS | 35.0% | |
| TTGATGCTACTCAACTTGAA+AGG | + | chr8.4:70812913-70812932 | MS.gene067337:CDS | 35.0% | |
| ACATTTGGGCCATTACAGTT+GGG | + | chr8.4:70812979-70812998 | MS.gene067337:CDS | 40.0% | |
| ATGCTACTCAACTTGAAAGG+GGG | + | chr8.4:70812916-70812935 | MS.gene067337:CDS | 40.0% | |
| GATGCTACTCAACTTGAAAG+GGG | + | chr8.4:70812915-70812934 | MS.gene067337:CDS | 40.0% | |
| TCAACTTGAAAGGGGGATAT+TGG | + | chr8.4:70812923-70812942 | MS.gene067337:CDS | 40.0% | |
| TGTTCATGAGCTCCACATTT+GGG | + | chr8.4:70812965-70812984 | MS.gene067337:CDS | 40.0% | |
| ATTGGAGATGGAGGAAGTAG+TGG | + | chr8.4:70812941-70812960 | MS.gene067337:CDS | 45.0% | |
| CAACTGTAATGGCCCAAATG+TGG | - | chr8.4:70812980-70812999 | None:intergenic | 45.0% | |
| CACATTTGGGCCATTACAGT+TGG | + | chr8.4:70812978-70812997 | MS.gene067337:CDS | 45.0% | |
| CTGTTCATGAGCTCCACATT+TGG | + | chr8.4:70812964-70812983 | MS.gene067337:CDS | 45.0% | |
| GTCCAACACCATTTCTGCAT+CGG | - | chr8.4:70813045-70813064 | None:intergenic | 45.0% | |
| TGAAAGGGGGATATTGGAGA+TGG | + | chr8.4:70812929-70812948 | MS.gene067337:CDS | 45.0% | |
| TGAGTAGCATCAATCTCTCG+AGG | - | chr8.4:70812906-70812925 | None:intergenic | 45.0% | |
| ! | TGCAGAAATGGTGTTGGACA+AGG | + | chr8.4:70813046-70813065 | MS.gene067337:CDS | 45.0% |
| !! | CATCGCGGTTGATTTTCACA+TGG | - | chr8.4:70813022-70813041 | None:intergenic | 45.0% |
| AAGGGGGATATTGGAGATGG+AGG | + | chr8.4:70812932-70812951 | MS.gene067337:CDS | 50.0% | |
| TGCCGATGCAGAAATGGTGT+TGG | + | chr8.4:70813040-70813059 | MS.gene067337:CDS | 50.0% | |
| ! | CCATTTCTGCATCGGCATCG+CGG | - | chr8.4:70813037-70813056 | None:intergenic | 55.0% |
| CCGCGATGCCGATGCAGAAA+TGG | + | chr8.4:70813034-70813053 | MS.gene067337:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.4 | gene | 70812864 | 70813106 | 70812864 | ID=MS.gene067337 |
| chr8.4 | mRNA | 70812864 | 70813106 | 70812864 | ID=MS.gene067337.t1;Parent=MS.gene067337 |
| chr8.4 | exon | 70812864 | 70813106 | 70812864 | ID=MS.gene067337.t1.exon1;Parent=MS.gene067337.t1 |
| chr8.4 | CDS | 70812864 | 70813106 | 70812864 | ID=cds.MS.gene067337.t1;Parent=MS.gene067337.t1 |
| Gene Sequence |
| Protein sequence |
