Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene067915.t1 | KEH20150.1 | 95.9 | 121 | 5 | 0 | 1 | 121 | 1 | 121 | 4.90E-06 | 60.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene067915.t1 | A0A072U2V7 | 95.9 | 121 | 5 | 0 | 1 | 121 | 1 | 121 | 3.5e-06 | 60.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene053549 | MS.gene067915 | 0.803055 | 4.18E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene067915.t1 | MTR_8g069315 | 86.992 | 123 | 13 | 2 | 1 | 121 | 1 | 122 | 3.98e-49 | 153 |
| MS.gene067915.t1 | MTR_8g069283 | 91.736 | 121 | 10 | 0 | 1 | 121 | 1 | 121 | 6.84e-46 | 144 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 42 sgRNAs with CRISPR-Local
Find 45 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGCCCACCTGGGCCTGATGC+TGG | 0.216285 | 8.3:+33813518 | None:intergenic |
| GGGGAAGATGTGTTTGGGCT+TGG | 0.239614 | 8.3:+33813563 | None:intergenic |
| GGGAAGATGTGTTTGGGCTT+GGG | 0.240843 | 8.3:+33813564 | None:intergenic |
| GTTTGTGATATTAGGTCTTT+TGG | 0.255388 | 8.3:-33813726 | MS.gene067915:CDS |
| TGCTCTAGTCCGGGCCCACC+TGG | 0.276732 | 8.3:+33813506 | None:intergenic |
| ATCAAATTGTTTGTGATATT+AGG | 0.276758 | 8.3:-33813734 | MS.gene067915:CDS |
| GCTGGTGGGGAAGATGTGTT+TGG | 0.322107 | 8.3:+33813557 | None:intergenic |
| GATGGAGCTGGTGCTGAATT+TGG | 0.324202 | 8.3:+33813650 | None:intergenic |
| AGCTCCTGGCTGCTCTAGTC+CGG | 0.331077 | 8.3:+33813496 | None:intergenic |
| TGCTGGAATCTTTGTGTCCA+TGG | 0.342466 | 8.3:-33813417 | MS.gene067915:CDS |
| GATGTGTTTGGGCTTGGGCC+TGG | 0.365493 | 8.3:+33813569 | None:intergenic |
| CCTTCAACTTCTTCTTCTCC+AGG | 0.374999 | 8.3:-33813587 | MS.gene067915:CDS |
| GCCCACCTGGGCCTGATGCT+GGG | 0.385695 | 8.3:+33813519 | None:intergenic |
| GCAGAGGGTGACTCAGCTCC+TGG | 0.403105 | 8.3:+33813482 | None:intergenic |
| CTATTAGAGCCTGGTGCTGG+TGG | 0.429246 | 8.3:+33813542 | None:intergenic |
| GAAGTTGAAGGAGATATTGC+AGG | 0.434197 | 8.3:+33813599 | None:intergenic |
| CTGGTGGGGAAGATGTGTTT+GGG | 0.437212 | 8.3:+33813558 | None:intergenic |
| TGAAGGAGATATTGCAGGAG+AGG | 0.441071 | 8.3:+33813604 | None:intergenic |
| GATATTGCAGGAGAGGTTGT+AGG | 0.444704 | 8.3:+33813611 | None:intergenic |
| TATTAGAGCCTGGTGCTGGT+GGG | 0.447878 | 8.3:+33813543 | None:intergenic |
| GGCTCTAATAGCCCAGCATC+AGG | 0.456286 | 8.3:-33813530 | MS.gene067915:CDS |
| GCATCAGGCCCAGGTGGGCC+CGG | 0.462450 | 8.3:-33813515 | MS.gene067915:CDS |
| GCTCCTGGCTGCTCTAGTCC+GGG | 0.489743 | 8.3:+33813497 | None:intergenic |
| GGGCTATTAGAGCCTGGTGC+TGG | 0.490149 | 8.3:+33813539 | None:intergenic |
| ATTAGAGCCTGGTGCTGGTG+GGG | 0.496717 | 8.3:+33813544 | None:intergenic |
| GCTCTAGTCCGGGCCCACCT+GGG | 0.506944 | 8.3:+33813507 | None:intergenic |
| GATGCTGGGCTATTAGAGCC+TGG | 0.508970 | 8.3:+33813533 | None:intergenic |
| GATTGTGTGGGTGATGGTGC+AGG | 0.539483 | 8.3:+33813680 | None:intergenic |
| CCTGGAGAAGAAGAAGTTGA+AGG | 0.541963 | 8.3:+33813587 | None:intergenic |
| GATAATGATTGTGTGGGTGA+TGG | 0.545354 | 8.3:+33813674 | None:intergenic |
| AATAGCCCAGCATCAGGCCC+AGG | 0.552354 | 8.3:-33813524 | MS.gene067915:CDS |
| AGCCCAGCATCAGGCCCAGG+TGG | 0.566917 | 8.3:-33813521 | MS.gene067915:CDS |
| GGGCCCGGACTAGAGCAGCC+AGG | 0.581693 | 8.3:-33813500 | MS.gene067915:CDS |
| ACCCATCTAAGCTAAAACCA+TGG | 0.581812 | 8.3:+33813400 | None:intergenic |
| TTTGTGGAAGGTGATGGAGC+TGG | 0.593413 | 8.3:+33813638 | None:intergenic |
| ATTTGGTGATAATGATTGTG+TGG | 0.597910 | 8.3:+33813667 | None:intergenic |
| AGTAACAGCAACAAAAGTGT+TGG | 0.609923 | 8.3:+33813445 | None:intergenic |
| ACATCTTCCCCACCAGCACC+AGG | 0.610370 | 8.3:-33813551 | MS.gene067915:CDS |
| GTGATAGAGAATGCAGCAGA+GGG | 0.615284 | 8.3:+33813467 | None:intergenic |
| GCCCAGCATCAGGCCCAGGT+GGG | 0.625332 | 8.3:-33813520 | MS.gene067915:CDS |
| TTTGGTGATAATGATTGTGT+GGG | 0.641938 | 8.3:+33813668 | None:intergenic |
| GGTGATAGAGAATGCAGCAG+AGG | 0.711837 | 8.3:+33813466 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATCAAATTGTTTGTGATATT+AGG | - | chr8.3:33813421-33813440 | MS.gene067915:CDS | 20.0% |
| ! | ATTTGGTGATAATGATTGTG+TGG | + | chr8.3:33813491-33813510 | None:intergenic | 30.0% |
| ! | GTTTGTGATATTAGGTCTTT+TGG | - | chr8.3:33813429-33813448 | MS.gene067915:CDS | 30.0% |
| ! | TTTGGTGATAATGATTGTGT+GGG | + | chr8.3:33813490-33813509 | None:intergenic | 30.0% |
| ! | AGTAACAGCAACAAAAGTGT+TGG | + | chr8.3:33813713-33813732 | None:intergenic | 35.0% |
| GAAGTTGAAGGAGATATTGC+AGG | + | chr8.3:33813559-33813578 | None:intergenic | 40.0% | |
| GATAATGATTGTGTGGGTGA+TGG | + | chr8.3:33813484-33813503 | None:intergenic | 40.0% | |
| ! | AGAGGTTGTAGGTGATTTTG+TGG | + | chr8.3:33813536-33813555 | None:intergenic | 40.0% |
| ! | GTTGTAGGTGATTTTGTGGA+AGG | + | chr8.3:33813532-33813551 | None:intergenic | 40.0% |
| CCTGGAGAAGAAGAAGTTGA+AGG | + | chr8.3:33813571-33813590 | None:intergenic | 45.0% | |
| CCTTCAACTTCTTCTTCTCC+AGG | - | chr8.3:33813568-33813587 | MS.gene067915:CDS | 45.0% | |
| GATATTGCAGGAGAGGTTGT+AGG | + | chr8.3:33813547-33813566 | None:intergenic | 45.0% | |
| GTGATAGAGAATGCAGCAGA+GGG | + | chr8.3:33813691-33813710 | None:intergenic | 45.0% | |
| TGAAGGAGATATTGCAGGAG+AGG | + | chr8.3:33813554-33813573 | None:intergenic | 45.0% | |
| TGCTGGAATCTTTGTGTCCA+TGG | - | chr8.3:33813738-33813757 | MS.gene067915:CDS | 45.0% | |
| ! | GGTGATTTTGTGGAAGGTGA+TGG | + | chr8.3:33813526-33813545 | None:intergenic | 45.0% |
| !! | GTTGCTGTTACTGCTTTTGC+TGG | - | chr8.3:33813721-33813740 | MS.gene067915:CDS | 45.0% |
| CTGGTGGGGAAGATGTGTTT+GGG | + | chr8.3:33813600-33813619 | None:intergenic | 50.0% | |
| GGGAAGATGTGTTTGGGCTT+GGG | + | chr8.3:33813594-33813613 | None:intergenic | 50.0% | |
| GGTGATAGAGAATGCAGCAG+AGG | + | chr8.3:33813692-33813711 | None:intergenic | 50.0% | |
| ! | GATGGAGCTGGTGCTGAATT+TGG | + | chr8.3:33813508-33813527 | None:intergenic | 50.0% |
| !! | TATTAGAGCCTGGTGCTGGT+GGG | + | chr8.3:33813615-33813634 | None:intergenic | 50.0% |
| !! | TTTGTGGAAGGTGATGGAGC+TGG | + | chr8.3:33813520-33813539 | None:intergenic | 50.0% |
| GATGCTGGGCTATTAGAGCC+TGG | + | chr8.3:33813625-33813644 | None:intergenic | 55.0% | |
| GCTGGTGGGGAAGATGTGTT+TGG | + | chr8.3:33813601-33813620 | None:intergenic | 55.0% | |
| GGGGAAGATGTGTTTGGGCT+TGG | + | chr8.3:33813595-33813614 | None:intergenic | 55.0% | |
| ! | CTATTAGAGCCTGGTGCTGG+TGG | + | chr8.3:33813616-33813635 | None:intergenic | 55.0% |
| ! | GATTGTGTGGGTGATGGTGC+AGG | + | chr8.3:33813478-33813497 | None:intergenic | 55.0% |
| ! | GGCTCTAATAGCCCAGCATC+AGG | - | chr8.3:33813625-33813644 | MS.gene067915:CDS | 55.0% |
| !! | ATTAGAGCCTGGTGCTGGTG+GGG | + | chr8.3:33813614-33813633 | None:intergenic | 55.0% |
| AATAGCCCAGCATCAGGCCC+AGG | - | chr8.3:33813631-33813650 | MS.gene067915:CDS | 60.0% | |
| ACATCTTCCCCACCAGCACC+AGG | - | chr8.3:33813604-33813623 | MS.gene067915:CDS | 60.0% | |
| AGCTCCTGGCTGCTCTAGTC+CGG | + | chr8.3:33813662-33813681 | None:intergenic | 60.0% | |
| GATGTGTTTGGGCTTGGGCC+TGG | + | chr8.3:33813589-33813608 | None:intergenic | 60.0% | |
| GGGCTATTAGAGCCTGGTGC+TGG | + | chr8.3:33813619-33813638 | None:intergenic | 60.0% | |
| GCAGAGGGTGACTCAGCTCC+TGG | + | chr8.3:33813676-33813695 | None:intergenic | 65.0% | |
| GCTCCTGGCTGCTCTAGTCC+GGG | + | chr8.3:33813661-33813680 | None:intergenic | 65.0% | |
| AGCCCAGCATCAGGCCCAGG+TGG | - | chr8.3:33813634-33813653 | MS.gene067915:CDS | 70.0% | |
| GCCCACCTGGGCCTGATGCT+GGG | + | chr8.3:33813639-33813658 | None:intergenic | 70.0% | |
| GCCCAGCATCAGGCCCAGGT+GGG | - | chr8.3:33813635-33813654 | MS.gene067915:CDS | 70.0% | |
| GCTCTAGTCCGGGCCCACCT+GGG | + | chr8.3:33813651-33813670 | None:intergenic | 70.0% | |
| TGCTCTAGTCCGGGCCCACC+TGG | + | chr8.3:33813652-33813671 | None:intergenic | 70.0% | |
| GCATCAGGCCCAGGTGGGCC+CGG | - | chr8.3:33813640-33813659 | MS.gene067915:CDS | 75.0% | |
| GGCCCACCTGGGCCTGATGC+TGG | + | chr8.3:33813640-33813659 | None:intergenic | 75.0% | |
| ! | GGGCCCGGACTAGAGCAGCC+AGG | - | chr8.3:33813655-33813674 | MS.gene067915:CDS | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.3 | gene | 33813406 | 33813771 | 33813406 | ID=MS.gene067915 |
| chr8.3 | mRNA | 33813406 | 33813771 | 33813406 | ID=MS.gene067915.t1;Parent=MS.gene067915 |
| chr8.3 | exon | 33813406 | 33813771 | 33813406 | ID=MS.gene067915.t1.exon1;Parent=MS.gene067915.t1 |
| chr8.3 | CDS | 33813406 | 33813771 | 33813406 | ID=cds.MS.gene067915.t1;Parent=MS.gene067915.t1 |
| Gene Sequence |
| Protein sequence |
