Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene067916.t1 | XP_024627753.1 | 79.6 | 103 | 19 | 1 | 1 | 103 | 1 | 101 | 2.70E-32 | 147.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene067916.t1 | B7FH71 | 94.6 | 74 | 4 | 0 | 1 | 74 | 1 | 74 | 8.3e-28 | 132.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene055549 | MS.gene067916 | 0.804716 | 1.89E-49 | -1.69E-46 |
| MS.gene055550 | MS.gene067916 | 0.802937 | 4.43E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 40 sgRNAs with CRISPR-Local
Find 60 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCCCCACCTGGGCCTGATGC+TGG | 0.215084 | 8.3:+33787620 | MS.gene067916:CDS |
| GATGGAGCTGGTGCTGAATT+TGG | 0.295928 | 8.3:+33787758 | MS.gene067916:CDS |
| GAAGTTGAAGGAGGATTTGC+AGG | 0.313240 | 8.3:+33787707 | MS.gene067916:CDS |
| TGCTGGAATCTTTGTGTCCA+TGG | 0.342466 | 8.3:-33787519 | None:intergenic |
| CCCCACCTGGGCCTGATGCT+GGG | 0.358406 | 8.3:+33787621 | MS.gene067916:CDS |
| TGTCCCGGTGTGGCCCCACC+TGG | 0.378803 | 8.3:+33787608 | MS.gene067916:CDS |
| GGCTCAATTGTCCCAGCATC+AGG | 0.383419 | 8.3:-33787632 | None:intergenic |
| GCAGAGGGTGCATCAGCTCC+TGG | 0.383493 | 8.3:+33787584 | MS.gene067916:CDS |
| TTTGGGCTTGGGCTTGAGCC+TGG | 0.386921 | 8.3:+33787677 | MS.gene067916:CDS |
| CCTTCAACTTCTTCTTCACC+AGG | 0.408150 | 8.3:-33787695 | None:intergenic |
| GGGACAATTGAGCCTGGTGC+TGG | 0.434202 | 8.3:+33787641 | MS.gene067916:CDS |
| GGGGCCACACCGGGACAGCC+AGG | 0.442844 | 8.3:-33787602 | None:intergenic |
| GATTGTGTGGGTGCTGGTCC+AGG | 0.453602 | 8.3:+33787788 | MS.gene067916:CDS |
| GCATCAGCTCCTGGCTGTCC+CGG | 0.460777 | 8.3:+33787593 | MS.gene067916:CDS |
| GATAGTGATTGTGTGGGTGC+TGG | 0.477013 | 8.3:+33787782 | MS.gene067916:CDS |
| GGATTTGCAGGAGAGGGTGT+TGG | 0.479611 | 8.3:+33787719 | MS.gene067916:CDS |
| CTGGTCCAGGAGCTTGTGCT+AGG | 0.481816 | 8.3:+33787801 | MS.gene067916:CDS |
| TCATGCCTAGCACAAGCTCC+TGG | 0.490825 | 8.3:-33787806 | None:intergenic |
| GGCCCAGGTGGGGCCACACC+GGG | 0.491499 | 8.3:-33787611 | None:intergenic |
| TGAAGGAGGATTTGCAGGAG+AGG | 0.492810 | 8.3:+33787712 | MS.gene067916:CDS |
| TCCCAGCATCAGGCCCAGGT+GGG | 0.497867 | 8.3:-33787622 | None:intergenic |
| GAAGGAGGATTTGCAGGAGA+GGG | 0.498221 | 8.3:+33787713 | MS.gene067916:CDS |
| GTCCCGGTGTGGCCCCACCT+GGG | 0.505740 | 8.3:+33787609 | MS.gene067916:CDS |
| CCCAGCATCAGGCCCAGGTG+GGG | 0.510499 | 8.3:-33787621 | None:intergenic |
| TTTGTAGAGGGTGATGGAGC+TGG | 0.511371 | 8.3:+33787746 | MS.gene067916:CDS |
| GATGCTGGGACAATTGAGCC+TGG | 0.515568 | 8.3:+33787635 | MS.gene067916:CDS |
| AGGCCCAGGTGGGGCCACAC+CGG | 0.517610 | 8.3:-33787612 | None:intergenic |
| ATTTGGTGATAGTGATTGTG+TGG | 0.527810 | 8.3:+33787775 | MS.gene067916:CDS |
| CCTGGTGAAGAAGAAGTTGA+AGG | 0.541963 | 8.3:+33787695 | MS.gene067916:CDS |
| ATTGTCCCAGCATCAGGCCC+AGG | 0.542663 | 8.3:-33787626 | None:intergenic |
| GTCCCAGCATCAGGCCCAGG+TGG | 0.563718 | 8.3:-33787623 | None:intergenic |
| AGCTCCTGGCTGTCCCGGTG+TGG | 0.567948 | 8.3:+33787598 | MS.gene067916:CDS |
| ACAATTGAGCCTGGTGCTGG+TGG | 0.580825 | 8.3:+33787644 | MS.gene067916:CDS |
| AGTAACAGCAACAAAAGTGT+TGG | 0.609923 | 8.3:+33787547 | MS.gene067916:CDS |
| GTGATAGAGAATGCAGCAGA+GGG | 0.615284 | 8.3:+33787569 | MS.gene067916:CDS |
| TTTGGTGATAGTGATTGTGT+GGG | 0.627176 | 8.3:+33787776 | MS.gene067916:CDS |
| AGGAGCTTGTGCTAGGCATG+AGG | 0.631267 | 8.3:+33787808 | MS.gene067916:CDS |
| GGTGAAGAAGAAGTTGAAGG+AGG | 0.679655 | 8.3:+33787698 | MS.gene067916:CDS |
| TTATTAGTCACATCACACAC+CGG | 0.687850 | 8.3:-33788118 | None:intergenic |
| GGTGATAGAGAATGCAGCAG+AGG | 0.711837 | 8.3:+33787568 | MS.gene067916:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATCAAAAAATATATACAACA+TGG | - | chr8.3:33787879-33787898 | None:intergenic | 15.0% |
| !! | ATCAAATTATTTGTGATATT+AGG | - | chr8.3:33787845-33787864 | None:intergenic | 15.0% |
| !! | TCAAAAAATATATACAACAT+GGG | - | chr8.3:33787878-33787897 | None:intergenic | 15.0% |
| !!! | GATGAATATTTTTTATGAAA+AGG | + | chr8.3:33787938-33787957 | MS.gene067916:intron | 15.0% |
| !! | AAATGATGAGTTAATAGTTA+AGG | + | chr8.3:33788002-33788021 | MS.gene067916:intron | 20.0% |
| ! | GCATTTACAAACCATATATT+TGG | - | chr8.3:33788039-33788058 | None:intergenic | 25.0% |
| ! | TTTACAAACCATATATTTGG+AGG | - | chr8.3:33788036-33788055 | None:intergenic | 25.0% |
| !! | ATTTGTGATATTAGGTCTTT+TGG | - | chr8.3:33787837-33787856 | None:intergenic | 25.0% |
| CTAAGATTCCTCCAAATATA+TGG | + | chr8.3:33788025-33788044 | MS.gene067916:intron | 30.0% | |
| GAGAGAGAATATATAAGAAG+AGG | + | chr8.3:33787963-33787982 | MS.gene067916:intron | 30.0% | |
| ! | ATGGTTTGTAAATGCATAAG+TGG | + | chr8.3:33788044-33788063 | MS.gene067916:intron | 30.0% |
| ! | AGTAACAGCAACAAAAGTGT+TGG | + | chr8.3:33787547-33787566 | MS.gene067916:CDS | 35.0% |
| ! | ATTTGGTGATAGTGATTGTG+TGG | + | chr8.3:33787775-33787794 | MS.gene067916:CDS | 35.0% |
| ! | TTTGGTGATAGTGATTGTGT+GGG | + | chr8.3:33787776-33787795 | MS.gene067916:CDS | 35.0% |
| !!! | ATTGCTTGTGATTTTTAGCC+AGG | + | chr8.3:33788095-33788114 | MS.gene067916:intron | 35.0% |
| !! | CTGGTGGTGAAAAAGTGTTT+GGG | + | chr8.3:33787660-33787679 | MS.gene067916:CDS | 40.0% |
| !! | GTGAAAAAGTGTTTGGGCTT+GGG | + | chr8.3:33787666-33787685 | MS.gene067916:CDS | 40.0% |
| !!! | GGTGTTGGTGATTTTGTAGA+GGG | + | chr8.3:33787734-33787753 | MS.gene067916:CDS | 40.0% |
| CCTGGTGAAGAAGAAGTTGA+AGG | + | chr8.3:33787695-33787714 | MS.gene067916:CDS | 45.0% | |
| CCTTCAACTTCTTCTTCACC+AGG | - | chr8.3:33787698-33787717 | None:intergenic | 45.0% | |
| GAAGTTGAAGGAGGATTTGC+AGG | + | chr8.3:33787707-33787726 | MS.gene067916:CDS | 45.0% | |
| GGTGAAGAAGAAGTTGAAGG+AGG | + | chr8.3:33787698-33787717 | MS.gene067916:CDS | 45.0% | |
| GTGATAGAGAATGCAGCAGA+GGG | + | chr8.3:33787569-33787588 | MS.gene067916:CDS | 45.0% | |
| ! | GGTGATTTTGTAGAGGGTGA+TGG | + | chr8.3:33787740-33787759 | MS.gene067916:CDS | 45.0% |
| !! | GCTGGTGGTGAAAAAGTGTT+TGG | + | chr8.3:33787659-33787678 | MS.gene067916:CDS | 45.0% |
| !! | GGTGAAAAAGTGTTTGGGCT+TGG | + | chr8.3:33787665-33787684 | MS.gene067916:CDS | 45.0% |
| !! | GTTGCTGTTACTGCTTTTGC+TGG | - | chr8.3:33787539-33787558 | None:intergenic | 45.0% |
| !!! | CTTGTGATTTTTAGCCAGGC+CGG | + | chr8.3:33788099-33788118 | MS.gene067916:intron | 45.0% |
| !!! | GGGTGTTGGTGATTTTGTAG+AGG | + | chr8.3:33787733-33787752 | MS.gene067916:CDS | 45.0% |
| GAAGGAGGATTTGCAGGAGA+GGG | + | chr8.3:33787713-33787732 | MS.gene067916:CDS | 50.0% | |
| GATAGTGATTGTGTGGGTGC+TGG | + | chr8.3:33787782-33787801 | MS.gene067916:CDS | 50.0% | |
| GGTGATAGAGAATGCAGCAG+AGG | + | chr8.3:33787568-33787587 | MS.gene067916:CDS | 50.0% | |
| TGAAGGAGGATTTGCAGGAG+AGG | + | chr8.3:33787712-33787731 | MS.gene067916:CDS | 50.0% | |
| ! | GATGGAGCTGGTGCTGAATT+TGG | + | chr8.3:33787758-33787777 | MS.gene067916:CDS | 50.0% |
| !! | TTTGTAGAGGGTGATGGAGC+TGG | + | chr8.3:33787746-33787765 | MS.gene067916:CDS | 50.0% |
| !!! | ACTTTTTCACCACCAGCACC+AGG | - | chr8.3:33787656-33787675 | None:intergenic | 50.0% |
| AGGAGCTTGTGCTAGGCATG+AGG | + | chr8.3:33787808-33787827 | MS.gene067916:CDS | 55.0% | |
| GATGCTGGGACAATTGAGCC+TGG | + | chr8.3:33787635-33787654 | MS.gene067916:CDS | 55.0% | |
| GGCTCAATTGTCCCAGCATC+AGG | - | chr8.3:33787635-33787654 | None:intergenic | 55.0% | |
| TCATGCCTAGCACAAGCTCC+TGG | - | chr8.3:33787809-33787828 | None:intergenic | 55.0% | |
| ! | ACAATTGAGCCTGGTGCTGG+TGG | + | chr8.3:33787644-33787663 | MS.gene067916:CDS | 55.0% |
| ! | GGATTTGCAGGAGAGGGTGT+TGG | + | chr8.3:33787719-33787738 | MS.gene067916:CDS | 55.0% |
| ATTGTCCCAGCATCAGGCCC+AGG | - | chr8.3:33787629-33787648 | None:intergenic | 60.0% | |
| CTGGTCCAGGAGCTTGTGCT+AGG | + | chr8.3:33787801-33787820 | MS.gene067916:CDS | 60.0% | |
| GGGACAATTGAGCCTGGTGC+TGG | + | chr8.3:33787641-33787660 | MS.gene067916:CDS | 60.0% | |
| TTTGGGCTTGGGCTTGAGCC+TGG | + | chr8.3:33787677-33787696 | MS.gene067916:CDS | 60.0% | |
| !! | GATTGTGTGGGTGCTGGTCC+AGG | + | chr8.3:33787788-33787807 | MS.gene067916:CDS | 60.0% |
| GCATCAGCTCCTGGCTGTCC+CGG | + | chr8.3:33787593-33787612 | MS.gene067916:CDS | 65.0% | |
| TCCCAGCATCAGGCCCAGGT+GGG | - | chr8.3:33787625-33787644 | None:intergenic | 65.0% | |
| ! | GCAGAGGGTGCATCAGCTCC+TGG | + | chr8.3:33787584-33787603 | MS.gene067916:CDS | 65.0% |
| AGCTCCTGGCTGTCCCGGTG+TGG | + | chr8.3:33787598-33787617 | MS.gene067916:CDS | 70.0% | |
| CCCAGCATCAGGCCCAGGTG+GGG | - | chr8.3:33787624-33787643 | None:intergenic | 70.0% | |
| CCCCACCTGGGCCTGATGCT+GGG | + | chr8.3:33787621-33787640 | MS.gene067916:CDS | 70.0% | |
| GTCCCAGCATCAGGCCCAGG+TGG | - | chr8.3:33787626-33787645 | None:intergenic | 70.0% | |
| AGGCCCAGGTGGGGCCACAC+CGG | - | chr8.3:33787615-33787634 | None:intergenic | 75.0% | |
| GCCCCACCTGGGCCTGATGC+TGG | + | chr8.3:33787620-33787639 | MS.gene067916:CDS | 75.0% | |
| GTCCCGGTGTGGCCCCACCT+GGG | + | chr8.3:33787609-33787628 | MS.gene067916:CDS | 75.0% | |
| TGTCCCGGTGTGGCCCCACC+TGG | + | chr8.3:33787608-33787627 | MS.gene067916:CDS | 75.0% | |
| ! | GGCCCAGGTGGGGCCACACC+GGG | - | chr8.3:33787614-33787633 | None:intergenic | 80.0% |
| ! | GGGGCCACACCGGGACAGCC+AGG | - | chr8.3:33787605-33787624 | None:intergenic | 80.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.3 | gene | 33787521 | 33788128 | 33787521 | ID=MS.gene067916 |
| chr8.3 | mRNA | 33787521 | 33788128 | 33787521 | ID=MS.gene067916.t1;Parent=MS.gene067916 |
| chr8.3 | exon | 33787521 | 33787829 | 33787521 | ID=MS.gene067916.t1.exon1;Parent=MS.gene067916.t1 |
| chr8.3 | CDS | 33787521 | 33787829 | 33787521 | ID=cds.MS.gene067916.t1;Parent=MS.gene067916.t1 |
| chr8.3 | exon | 33788117 | 33788128 | 33788117 | ID=MS.gene067916.t1.exon2;Parent=MS.gene067916.t1 |
| chr8.3 | CDS | 33788117 | 33788128 | 33788117 | ID=cds.MS.gene067916.t1;Parent=MS.gene067916.t1 |
| Gene Sequence |
| Protein sequence |
