AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene06811

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene06811.t1 MTR_3g092890 99.160 119 1 0 1 119 1 119 2.33e-82 245
MS.gene06811.t1 MTR_8g024120 50.000 118 53 3 6 119 11 126 6.66e-31 113
MS.gene06811.t1 MTR_7g016090 39.062 128 76 2 1 127 1 127 4.41e-25 97.8
MS.gene06811.t1 MTR_7g016090 39.062 128 76 2 1 127 3 129 4.62e-25 97.8
MS.gene06811.t1 MTR_7g016080 39.231 130 75 3 1 127 1 129 1.13e-22 91.3
MS.gene06811.t1 MTR_7g081710 42.857 112 59 2 13 119 23 134 1.39e-21 88.6
MS.gene06811.t1 MTR_1g070135 33.846 130 82 2 1 127 1 129 6.05e-20 83.2
MS.gene06811.t1 MTR_1g070135 33.846 130 82 2 1 127 1 129 8.73e-20 83.6
MS.gene06811.t1 MTR_3g070860 39.496 119 63 4 6 117 18 134 3.88e-19 82.0
MS.gene06811.t1 MTR_5g032880 41.000 100 54 1 15 109 353 452 4.11e-19 82.4
MS.gene06811.t1 MTR_5g032880 45.263 95 49 2 15 109 15 106 1.36e-17 78.2
MS.gene06811.t1 MTR_1g062110 37.097 124 68 4 4 119 29 150 1.55e-18 79.7
MS.gene06811.t1 MTR_1g062110 37.097 124 68 4 4 119 29 150 1.67e-18 80.1
MS.gene06811.t1 MTR_1g048940 37.736 106 59 2 13 113 22 125 6.12e-18 78.6
MS.gene06811.t1 MTR_5g082750 38.182 110 60 3 15 117 25 133 6.64e-18 78.6
MS.gene06811.t1 MTR_5g032870 40.000 100 55 1 15 109 15 114 2.53e-17 76.6
MS.gene06811.t1 MTR_1g070120 33.945 109 68 2 15 119 15 123 3.52e-17 76.3
MS.gene06811.t1 MTR_1g070085 37.624 101 60 1 15 112 14 114 1.03e-16 75.1
MS.gene06811.t1 MTR_1g070085 37.624 101 60 1 15 112 14 114 1.03e-16 75.1
MS.gene06811.t1 MTR_7g102820 36.842 114 63 2 14 118 37 150 2.67e-16 74.3
MS.gene06811.t1 MTR_3g045230 35.135 111 68 1 15 121 29 139 3.73e-16 73.6
MS.gene06811.t1 MTR_7g102720 38.372 86 53 0 36 121 35 120 6.25e-16 72.8
MS.gene06811.t1 MTR_1g070080 37.168 113 67 2 4 112 3 115 8.26e-16 72.4
MS.gene06811.t1 MTR_6g015950 31.298 131 89 1 1 130 1 131 8.74e-16 72.4
MS.gene06811.t1 MTR_3g043900 38.462 104 59 2 15 114 29 131 1.33e-15 72.0
MS.gene06811.t1 MTR_1g034140 40.476 84 50 0 18 101 7 90 2.07e-15 71.6
MS.gene06811.t1 MTR_1g034060 40.625 96 45 3 17 101 6 100 4.99e-15 70.5
MS.gene06811.t1 MTR_1g034120 40.625 96 45 3 17 101 6 100 5.04e-15 70.5
MS.gene06811.t1 MTR_2g069300 40.964 83 44 1 38 115 54 136 3.03e-14 68.2
MS.gene06811.t1 MTR_7g068650 31.897 116 67 3 13 117 24 138 4.62e-14 67.8
MS.gene06811.t1 MTR_4g015790 32.576 132 62 5 8 117 20 146 5.11e-14 67.8
MS.gene06811.t1 MTR_5g059130 39.286 84 50 1 13 95 18 101 3.24e-13 65.5
MS.gene06811.t1 MTR_5g048850 35.965 114 64 5 13 121 22 131 4.59e-13 64.3
MS.gene06811.t1 MTR_4g087120 32.800 125 72 4 18 131 7 130 4.85e-13 64.7
MS.gene06811.t1 MTR_4g087090 34.545 110 46 2 36 135 28 121 5.13e-13 64.7
MS.gene06811.t1 MTR_5g048850 35.965 114 64 5 13 121 22 131 5.67e-13 64.7
MS.gene06811.t1 MTR_0009s0410 33.898 118 66 6 13 124 21 132 6.02e-13 62.4
MS.gene06811.t1 MTR_4g100600 45.161 62 34 0 37 98 8 69 6.60e-13 63.5
MS.gene06811.t1 MTR_5g055820 34.211 114 66 4 13 121 22 131 8.17e-13 64.3
MS.gene06811.t1 MTR_4g087100 39.583 96 48 3 18 104 7 101 8.74e-13 64.3
MS.gene06811.t1 MTR_4g100600 45.161 62 34 0 37 98 8 69 1.10e-12 63.5
MS.gene06811.t1 MTR_4g100590 46.774 62 33 0 37 98 8 69 1.41e-12 63.5
MS.gene06811.t1 MTR_2g033270 37.349 83 51 1 37 119 18 99 1.89e-12 63.2
MS.gene06811.t1 MTR_7g063730 36.782 87 52 2 14 97 39 125 2.67e-12 62.8
MS.gene06811.t1 MTR_5g055680 37.500 88 51 2 14 98 23 109 2.91e-12 62.8
MS.gene06811.t1 MTR_1g032250 30.597 134 83 4 5 134 40 167 2.96e-12 62.8
MS.gene06811.t1 MTR_5g048550 34.831 89 54 2 14 99 23 110 2.96e-12 62.8
MS.gene06811.t1 MTR_5g055900 32.258 93 59 2 12 101 21 112 3.06e-12 62.8
MS.gene06811.t1 MTR_0009s0050 36.364 88 54 1 14 99 23 110 3.31e-12 62.4
MS.gene06811.t1 MTR_0009s0050 36.364 88 54 1 14 99 23 110 3.51e-12 62.4
MS.gene06811.t1 MTR_2g069190 34.940 83 49 1 38 115 68 150 4.45e-12 62.0
MS.gene06811.t1 MTR_4g099380 40.789 76 45 0 33 108 169 244 4.59e-12 61.6
MS.gene06811.t1 MTR_4g087140 36.842 95 50 3 17 102 6 99 4.92e-12 62.0
MS.gene06811.t1 MTR_8g064200 36.522 115 63 5 13 121 27 137 4.97e-12 62.0
MS.gene06811.t1 MTR_8g064200 36.522 115 63 5 13 121 32 142 4.97e-12 62.0
MS.gene06811.t1 MTR_7g045650 35.632 87 53 2 14 97 35 121 5.85e-12 61.6
MS.gene06811.t1 MTR_0032s0040 29.710 138 83 5 5 132 31 164 5.93e-12 62.0
MS.gene06811.t1 MTR_5g059140 33.333 99 62 2 1 95 1 99 5.97e-12 61.6
MS.gene06811.t1 MTR_7g062570 36.264 91 55 2 10 97 26 116 6.17e-12 61.6
MS.gene06811.t1 MTR_3g058610 34.862 109 64 3 3 105 4 111 6.95e-12 61.6
MS.gene06811.t1 MTR_5g048470 38.462 117 59 7 13 121 27 138 7.65e-12 61.6
MS.gene06811.t1 MTR_0328s0030 30.973 113 73 3 13 122 20 130 8.42e-12 61.2
MS.gene06811.t1 MTR_1g032140 32.824 131 78 4 8 134 36 160 9.60e-12 61.2
MS.gene06811.t1 MTR_8g075890 35.955 89 52 1 15 98 16 104 1.05e-11 61.2
MS.gene06811.t1 MTR_4g093840 30.769 130 77 6 4 121 24 152 1.26e-11 60.8
MS.gene06811.t1 MTR_3g072810 35.165 91 57 2 14 104 16 104 1.45e-11 60.5
MS.gene06811.t1 MTR_5g055690 28.829 111 77 1 13 121 22 132 1.48e-11 60.8
MS.gene06811.t1 MTR_5g055690 28.829 111 77 1 13 121 22 132 1.49e-11 60.5
MS.gene06811.t1 MTR_1g032220 30.597 134 83 4 5 134 40 167 2.03e-11 60.5
MS.gene06811.t1 MTR_1g102070 34.951 103 56 4 1 97 27 124 2.23e-11 60.1
MS.gene06811.t1 MTR_4g100610 45.161 62 34 0 37 98 8 69 2.29e-11 59.3
MS.gene06811.t1 MTR_0009s0480 32.479 117 69 5 13 124 21 132 2.36e-11 59.7
MS.gene06811.t1 MTR_4g087130 35.185 108 60 3 18 116 7 113 2.45e-11 60.1
MS.gene06811.t1 MTR_8g028435 35.714 84 48 1 38 115 60 143 3.24e-11 59.7
MS.gene06811.t1 MTR_5g065010 33.058 121 73 3 13 127 14 132 3.40e-11 59.7
MS.gene06811.t1 MTR_5g022020 30.392 102 64 3 3 98 4 104 4.12e-11 59.3
MS.gene06811.t1 MTR_5g055800 32.955 88 57 1 14 99 23 110 4.45e-11 59.3
MS.gene06811.t1 MTR_2g072890 30.973 113 73 2 13 121 27 138 5.43e-11 58.9
MS.gene06811.t1 MTR_3g091360 35.714 98 54 4 37 127 51 146 5.80e-11 58.9
MS.gene06811.t1 MTR_2g069090 31.461 89 56 1 38 121 68 156 5.97e-11 58.9
MS.gene06811.t1 MTR_3g072820 35.366 82 52 1 15 95 18 99 6.55e-11 58.9
MS.gene06811.t1 MTR_8g009160 34.524 84 49 1 38 115 60 143 7.61e-11 58.5
MS.gene06811.t1 MTR_8g041600 36.047 86 48 1 37 115 67 152 8.33e-11 58.5
MS.gene06811.t1 MTR_8g075830 32.673 101 63 1 3 98 4 104 8.48e-11 58.5
MS.gene06811.t1 MTR_5g048740 30.841 107 64 3 2 99 3 108 8.67e-11 58.5
MS.gene06811.t1 MTR_8g041600 36.047 86 48 1 37 115 67 152 9.61e-11 58.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene06811.t1 AT5G24530 57.983 119 50 0 1 119 1 119 3.40e-45 150
MS.gene06811.t1 AT4G10500 50.833 120 52 3 6 121 14 130 1.54e-31 115
MS.gene06811.t1 AT4G10490 47.458 118 56 3 6 119 11 126 9.34e-28 105
MS.gene06811.t1 AT2G36690 40.000 115 60 2 13 118 30 144 4.92e-20 84.3
MS.gene06811.t1 AT2G36690 40.000 115 60 2 13 118 30 144 4.92e-20 84.3
MS.gene06811.t1 AT3G13610 39.450 109 64 2 15 121 38 146 6.23e-19 81.3
MS.gene06811.t1 AT2G44800 35.833 120 69 3 14 127 26 143 1.51e-17 77.4
MS.gene06811.t1 AT3G60290 37.815 119 66 4 15 127 27 143 8.12e-17 75.5
MS.gene06811.t1 AT5G07480 38.462 104 56 2 15 113 24 124 4.44e-16 73.6
MS.gene06811.t1 AT1G55290 36.364 110 67 2 15 121 37 146 5.07e-16 73.2
MS.gene06811.t1 AT5G12270 34.951 103 66 1 13 114 31 133 1.27e-15 72.0
MS.gene06811.t1 AT5G05600 37.069 116 65 3 13 121 36 150 1.12e-14 68.9
MS.gene06811.t1 AT3G11180 38.053 113 60 4 13 117 65 175 1.24e-14 69.7
MS.gene06811.t1 AT3G11180 38.053 113 60 4 13 117 65 175 1.24e-14 69.7
MS.gene06811.t1 AT3G19000 37.000 100 53 3 13 102 2 101 1.68e-14 68.6
MS.gene06811.t1 AT5G05600 37.069 116 65 3 13 121 36 150 1.90e-14 68.9
MS.gene06811.t1 AT3G19000 37.000 100 53 3 13 102 2 101 2.68e-14 68.6
MS.gene06811.t1 AT3G19010 43.662 71 36 1 36 102 26 96 1.22e-13 66.6
MS.gene06811.t1 AT3G19010 43.662 71 36 1 36 102 26 96 1.52e-13 66.2
MS.gene06811.t1 AT3G19010 43.662 71 36 1 36 102 26 96 1.52e-13 66.2
MS.gene06811.t1 AT3G19010 43.662 71 36 1 36 102 26 96 2.04e-13 65.9
MS.gene06811.t1 AT3G51240 33.981 103 63 1 1 98 1 103 2.71e-13 65.5
MS.gene06811.t1 AT3G12900 34.711 121 73 3 5 121 25 143 1.81e-12 63.2
MS.gene06811.t1 AT1G06620 37.500 88 49 2 33 115 58 144 1.98e-12 62.8
MS.gene06811.t1 AT1G06620 37.500 88 49 2 33 115 58 144 3.06e-12 62.8
MS.gene06811.t1 AT5G08640 30.435 92 63 1 5 95 9 100 1.27e-11 60.8
MS.gene06811.t1 AT5G08640 30.435 92 63 1 5 95 9 100 1.27e-11 60.8
MS.gene06811.t1 AT1G80340 34.940 83 51 1 15 97 27 106 1.37e-11 60.8
MS.gene06811.t1 AT4G25300 32.456 114 71 3 13 121 26 138 1.50e-11 60.8
MS.gene06811.t1 AT1G06650 29.752 121 73 3 5 115 29 147 1.54e-11 60.8
MS.gene06811.t1 AT1G06650 29.752 121 73 3 5 115 29 147 1.59e-11 60.5
MS.gene06811.t1 AT1G06640 32.231 121 70 3 5 115 29 147 1.61e-11 60.5
MS.gene06811.t1 AT1G04350 31.092 119 76 4 11 124 29 146 1.64e-11 60.5
MS.gene06811.t1 AT1G06640 32.231 121 70 3 5 115 29 147 1.73e-11 60.1
MS.gene06811.t1 AT1G06640 32.231 121 70 3 5 115 29 147 2.28e-11 60.1
MS.gene06811.t1 AT2G38240 29.091 110 74 3 15 121 23 131 3.17e-11 59.7
MS.gene06811.t1 AT5G51810 36.782 87 52 3 12 97 39 123 4.66e-11 59.3
MS.gene06811.t1 AT5G51810 36.782 87 52 3 12 97 39 123 5.43e-11 58.9
MS.gene06811.t1 AT1G17010 32.174 115 71 3 13 121 27 140 7.56e-11 58.5
MS.gene06811.t1 AT3G61400 39.506 81 39 1 37 107 59 139 8.68e-11 58.5

Find 21 sgRNAs with CRISPR-Local

Find 130 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAATGTTCCTATCATTGATT+TGG 0.233266 3.1:+74855287 MS.gene06811:CDS
TTCGGGTCTGATGTAGCTTT+CGG 0.284448 3.1:-74855226 None:intergenic
TCGGGTCTGATGTAGCTTTC+GGG 0.320790 3.1:-74855225 None:intergenic
TCTTCACGTTTAATGTCTTC+CGG 0.353789 3.1:-74857516 None:intergenic
GACACCAAAGTGCTTTCTTC+CGG 0.368362 3.1:+74855183 MS.gene06811:CDS
AATGTTCCTATCATTGATTT+GGG 0.394239 3.1:+74855288 MS.gene06811:CDS
TAACTTTGAATGATGGATTC+CGG 0.402470 3.1:-74855202 None:intergenic
ACACAGATTGTGCAGCAAAT+TGG 0.405459 3.1:+74855324 MS.gene06811:CDS
AAGACATGGTCTATCAGATT+CGG 0.410372 3.1:-74855244 None:intergenic
ATTGGACAGGTAGTTAATCA+TGG 0.442304 3.1:+74855778 MS.gene06811:intron
GGTGAAGCATGCAGCTCCTA+TGG 0.451140 3.1:+74855345 MS.gene06811:CDS
AGACATGGTCTATCAGATTC+GGG 0.453451 3.1:-74855243 None:intergenic
TGAGAACCCAAATCAATGAT+AGG 0.480759 3.1:-74855294 None:intergenic
TTTCGGGTAACTTTGAATGA+TGG 0.482331 3.1:-74855209 None:intergenic
TTGGACAGGTAGTTAATCAT+GGG 0.500876 3.1:+74855779 MS.gene06811:intron
GATAGTCTCATTGTCTTCGT+GGG 0.526905 3.1:-74855895 None:intergenic
AGATAGTCTCATTGTCTTCG+TGG 0.538477 3.1:-74855896 None:intergenic
TTATTCGATATGTGAAAGAC+CGG 0.570547 3.1:+74857497 MS.gene06811:CDS
GATTCCGGAAGAAAGCACTT+TGG 0.619711 3.1:-74855187 None:intergenic
TCAGAGACTTGAGAAAGACA+TGG 0.682588 3.1:-74855258 None:intergenic
TGGACAGGTAGTTAATCATG+GGG 0.699574 3.1:+74855780 MS.gene06811:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAAATAATTATAAATAT+AGG - chr3.1:74857447-74857466 None:intergenic 0.0%
!!! TTATTTTATTTTTTATTTAT+CGG + chr3.1:74857205-74857224 MS.gene06811:intron 0.0%
!! AAACAAAAAATTGTAATATT+GGG - chr3.1:74855387-74855406 None:intergenic 10.0%
!! CAAATAACATAATTATTTAA+AGG + chr3.1:74856981-74857000 MS.gene06811:intron 10.0%
!! CTATTTATTATAATTAGAAT+AGG + chr3.1:74855478-74855497 MS.gene06811:intron 10.0%
!! TAAACAAAAAATTGTAATAT+TGG - chr3.1:74855388-74855407 None:intergenic 10.0%
!!! ATTTTAAAATTTCCTTTTTT+AGG - chr3.1:74857046-74857065 None:intergenic 10.0%
!! AAAAAAATGTGGAAATTAAA+GGG - chr3.1:74857321-74857340 None:intergenic 15.0%
!! AAGATAAATCAAAAAGAATA+TGG - chr3.1:74857270-74857289 None:intergenic 15.0%
!! AGATAAATCAAAAAGAATAT+GGG - chr3.1:74857269-74857288 None:intergenic 15.0%
!! GAAAATATTTGAAAACTTAA+AGG + chr3.1:74856475-74856494 MS.gene06811:intron 15.0%
!! TAAAAAAATGTGGAAATTAA+AGG - chr3.1:74857322-74857341 None:intergenic 15.0%
!! TAACAAAAGTTGTTAAAAAA+TGG - chr3.1:74856834-74856853 None:intergenic 15.0%
!!! ACAAGTTATTTTTATAAACA+AGG - chr3.1:74856180-74856199 None:intergenic 15.0%
!!! AGTAGAACATAAATATTTTT+AGG - chr3.1:74856765-74856784 None:intergenic 15.0%
!!! CAAGTTATTTTTATAAACAA+GGG - chr3.1:74856179-74856198 None:intergenic 15.0%
!!! GATTAAATAAAATGTCATTT+TGG + chr3.1:74855629-74855648 MS.gene06811:intron 15.0%
!!! GTAGAACATAAATATTTTTA+GGG - chr3.1:74856764-74856783 None:intergenic 15.0%
!! AATTGTATTTAGTAGAAATG+AGG - chr3.1:74855516-74855535 None:intergenic 20.0%
!! ATATTGTTAATTAATTGGAC+AGG + chr3.1:74855765-74855784 MS.gene06811:intron 20.0%
!! CATAACTTTACAAAGAAATT+GGG - chr3.1:74856928-74856947 None:intergenic 20.0%
!! GATAAATCAAAAAGAATATG+GGG - chr3.1:74857268-74857287 None:intergenic 20.0%
!!! AAATGTAGAAAGTATTACAT+TGG - chr3.1:74857115-74857134 None:intergenic 20.0%
!!! ATACTTTTCGTAAAAAAGTA+TGG + chr3.1:74857362-74857381 MS.gene06811:intron 20.0%
!!! ATCATCGTTTTTGTAATATT+TGG - chr3.1:74856436-74856455 None:intergenic 20.0%
!!! CAATGAAGATTATTTTAGAT+AGG + chr3.1:74855437-74855456 MS.gene06811:intron 20.0%
!!! CACATAATTTTTTAAGTTTG+AGG + chr3.1:74856323-74856342 MS.gene06811:intron 20.0%
!!! CTTTTTGTAAAAAAAATCGA+TGG - chr3.1:74857473-74857492 None:intergenic 20.0%
!!! TACTTTTCGTAAAAAAGTAT+GGG + chr3.1:74857363-74857382 MS.gene06811:intron 20.0%
!!! TTTTTTATTTATCGGTTTAG+TGG + chr3.1:74857213-74857232 MS.gene06811:intron 20.0%
! ATATTACAAAAACGATGATG+TGG + chr3.1:74856437-74856456 MS.gene06811:intron 25.0%
! ATGTGATCCAAATAACTTAA+AGG - chr3.1:74856365-74856384 None:intergenic 25.0%
! CCAATTTCTTTGTAAAGTTA+TGG + chr3.1:74856926-74856945 MS.gene06811:intron 25.0%
! CCATAACTTTACAAAGAAAT+TGG - chr3.1:74856929-74856948 None:intergenic 25.0%
! TAATCACTCAAAATTGAACT+AGG - chr3.1:74855614-74855633 None:intergenic 25.0%
! TAATCTTCATTGTTCATCTA+TGG - chr3.1:74855429-74855448 None:intergenic 25.0%
! TATTTCGTATACATATAGCT+AGG - chr3.1:74857391-74857410 None:intergenic 25.0%
!! AAATGTTCCTATCATTGATT+TGG + chr3.1:74855287-74855306 MS.gene06811:CDS 25.0%
!! ATATTAAACACCGTAAGTTT+TGG + chr3.1:74855729-74855748 MS.gene06811:intron 25.0%
!! ATTTTTCATTTGAGTATGTG+TGG - chr3.1:74857239-74857258 None:intergenic 25.0%
!! TATTAAACACCGTAAGTTTT+GGG + chr3.1:74855730-74855749 MS.gene06811:intron 25.0%
!! TTTTTACCAGGGAAATTAAA+TGG + chr3.1:74857336-74857355 MS.gene06811:intron 25.0%
!!! AAGTTTTAATGTGAACAAAG+AGG + chr3.1:74855921-74855940 MS.gene06811:CDS 25.0%
!!! AATGTTCCTATCATTGATTT+GGG + chr3.1:74855288-74855307 MS.gene06811:CDS 25.0%
!!! AATTTCCACATTTTTTTACC+AGG + chr3.1:74857324-74857343 MS.gene06811:intron 25.0%
!!! ACTTTTCGTAAAAAAGTATG+GGG + chr3.1:74857364-74857383 MS.gene06811:intron 25.0%
!!! ATTTCCACATTTTTTTACCA+GGG + chr3.1:74857325-74857344 MS.gene06811:intron 25.0%
!!! GTTTTTTTCAATTCTTCTAG+AGG - chr3.1:74855808-74855827 None:intergenic 25.0%
!!! TTTACATCATTTTAGCCTTT+AGG + chr3.1:74857288-74857307 MS.gene06811:intron 25.0%
ATTGAAAAAAACAGCAGAAG+TGG + chr3.1:74855816-74855835 MS.gene06811:CDS 30.0%
ATTTAAAGGGTCATGTTAAC+CGG + chr3.1:74856995-74857014 MS.gene06811:intron 30.0%
ATTTCCCTGGTAAAAAAATG+TGG - chr3.1:74857332-74857351 None:intergenic 30.0%
CATGTATAAAACATGTCACA+AGG - chr3.1:74856129-74856148 None:intergenic 30.0%
CTGATTAAGGAACCTAAAAA+AGG + chr3.1:74857031-74857050 MS.gene06811:intron 30.0%
GTCATAAGTTGTCTATCAAA+TGG + chr3.1:74856953-74856972 MS.gene06811:intron 30.0%
GTTACAAACCTCAAACTTAA+AGG + chr3.1:74856530-74856549 MS.gene06811:intron 30.0%
TAGATAAAAGAGATACCGTT+AGG + chr3.1:74857409-74857428 MS.gene06811:intron 30.0%
TTATTCGATATGTGAAAGAC+CGG + chr3.1:74857497-74857516 MS.gene06811:CDS 30.0%
! AATTAGTCTCCCAAAACTTA+CGG - chr3.1:74855742-74855761 None:intergenic 30.0%
! ACATAAGTCCTTTAAGTTTG+AGG - chr3.1:74856541-74856560 None:intergenic 30.0%
! GTTTGAGGTTTATAATAGAG+TGG + chr3.1:74856338-74856357 MS.gene06811:intron 30.0%
! GTTTTCTTTCTCTATTGCTT+TGG + chr3.1:74856887-74856906 MS.gene06811:intron 30.0%
! TATATGCCATTTAATTTCCC+TGG - chr3.1:74857345-74857364 None:intergenic 30.0%
! TTAAGTTTGAGGTTTGTAAC+TGG - chr3.1:74856530-74856549 None:intergenic 30.0%
! TTTTAATGTGAACAAAGAGG+AGG + chr3.1:74855924-74855943 MS.gene06811:CDS 30.0%
!! TAACTTTGAATGATGGATTC+CGG - chr3.1:74855205-74855224 None:intergenic 30.0%
AAGACATGGTCTATCAGATT+CGG - chr3.1:74855247-74855266 None:intergenic 35.0%
AAGGATAGCAATGAAGTCTA+AGG - chr3.1:74855969-74855988 None:intergenic 35.0%
ACAGAGAAAAATGAAGAGAG+TGG - chr3.1:74856044-74856063 None:intergenic 35.0%
ATAAAACATGTCACAAGGTG+AGG - chr3.1:74856124-74856143 None:intergenic 35.0%
ATTGGACAGGTAGTTAATCA+TGG + chr3.1:74855778-74855797 MS.gene06811:intron 35.0%
CTCAAAATTGAACTAGGTTC+TGG - chr3.1:74855608-74855627 None:intergenic 35.0%
TAGACTTCATTGCTATCCTT+TGG + chr3.1:74855969-74855988 MS.gene06811:intron 35.0%
TCTGGCACATAATTATCCAA+AGG - chr3.1:74855988-74856007 None:intergenic 35.0%
TCTTCACGTTTAATGTCTTC+CGG - chr3.1:74857519-74857538 None:intergenic 35.0%
TGAGAACCCAAATCAATGAT+AGG - chr3.1:74855297-74855316 None:intergenic 35.0%
TGGATAATTATGTGCCAGAA+TGG + chr3.1:74855989-74856008 MS.gene06811:intron 35.0%
TTGAATGAAGGAGGATTAGA+AGG - chr3.1:74856015-74856034 None:intergenic 35.0%
TTGGACAGGTAGTTAATCAT+GGG + chr3.1:74855779-74855798 MS.gene06811:intron 35.0%
! GAGTGGTCCTTTAAGTTATT+TGG + chr3.1:74856355-74856374 MS.gene06811:intron 35.0%
! GTGATGATGTATTCATCATG+AGG + chr3.1:74856148-74856167 MS.gene06811:intron 35.0%
! TTTCGGGTAACTTTGAATGA+TGG - chr3.1:74855212-74855231 None:intergenic 35.0%
!!! TGATTTTTTCAAGTTGCCAG+TGG + chr3.1:74855843-74855862 MS.gene06811:CDS 35.0%
ACACAGATTGTGCAGCAAAT+TGG + chr3.1:74855324-74855343 MS.gene06811:CDS 40.0%
AGACATGGTCTATCAGATTC+GGG - chr3.1:74855246-74855265 None:intergenic 40.0%
AGATAGTCTCATTGTCTTCG+TGG - chr3.1:74855899-74855918 None:intergenic 40.0%
AGGAGCTGAAAATGACCTAA+CGG - chr3.1:74857427-74857446 None:intergenic 40.0%
CTAGACCTGGAAAAAACCAT+AGG - chr3.1:74855364-74855383 None:intergenic 40.0%
GATAGTCTCATTGTCTTCGT+GGG - chr3.1:74855898-74855917 None:intergenic 40.0%
TCAGAGACTTGAGAAAGACA+TGG - chr3.1:74855261-74855280 None:intergenic 40.0%
TGGACAGGTAGTTAATCATG+GGG + chr3.1:74855780-74855799 MS.gene06811:intron 40.0%
TGGACTTACTTGAATGAAGG+AGG - chr3.1:74856024-74856043 None:intergenic 40.0%
TTCAGCCACGTATCTATCAT+CGG + chr3.1:74856073-74856092 MS.gene06811:intron 40.0%
! AACTTCATTTTCTCCTCCAC+TGG - chr3.1:74855862-74855881 None:intergenic 40.0%
! ACAAAGAGGAGGTGCATAAT+TGG + chr3.1:74855935-74855954 MS.gene06811:intron 40.0%
! GAGTGGACTTACTTGAATGA+AGG - chr3.1:74856027-74856046 None:intergenic 40.0%
! TTGTAATATTGGGCTAGACC+TGG - chr3.1:74855377-74855396 None:intergenic 40.0%
!!! TTTTTTCAAGTTGCCAGTGG+AGG + chr3.1:74855846-74855865 MS.gene06811:CDS 40.0%
ACCACTTAGGTTAGAACGAG+TGG - chr3.1:74855564-74855583 None:intergenic 45.0%
CTTGTCCGATGATAGATACG+TGG - chr3.1:74856081-74856100 None:intergenic 45.0%
TTCGGGTCTGATGTAGCTTT+CGG - chr3.1:74855229-74855248 None:intergenic 45.0%
! AAGAGGAGGTGCATAATTGG+AGG + chr3.1:74855938-74855957 MS.gene06811:intron 45.0%
! AAGTGTCACTGACTAGGTCT+AGG + chr3.1:74856098-74856117 MS.gene06811:intron 45.0%
! AGAGGAGGTGCATAATTGGA+GGG + chr3.1:74855939-74855958 MS.gene06811:intron 45.0%
! GACACCAAAGTGCTTTCTTC+CGG + chr3.1:74855183-74855202 MS.gene06811:CDS 45.0%
! TTAAAGGGCATGCAGCCTAA+AGG - chr3.1:74857306-74857325 None:intergenic 45.0%
!! GATTCCGGAAGAAAGCACTT+TGG - chr3.1:74855190-74855209 None:intergenic 45.0%
!!! CAGCTCCTATGGTTTTTTCC+AGG + chr3.1:74855356-74855375 MS.gene06811:CDS 45.0%
!! AAATAACATAATTATTTAAA+GGG + chr3.1:74856982-74857001 MS.gene06811:intron 5.0%
!! TTAATATATTGTTAATTAAT+TGG + chr3.1:74855760-74855779 MS.gene06811:intron 5.0%
!!! AAAATTTACTTTTTAAATAA+GGG - chr3.1:74857153-74857172 None:intergenic 5.0%
!!! TAAAATTTACTTTTTAAATA+AGG - chr3.1:74857154-74857173 None:intergenic 5.0%
!!! TTTAAGATAATTTTTTTTTA+GGG - chr3.1:74856674-74856693 None:intergenic 5.0%
!!! TTTTAAGATAATTTTTTTTT+AGG - chr3.1:74856675-74856694 None:intergenic 5.0%
ACACTGCGCATCAACCACTT+AGG - chr3.1:74855577-74855596 None:intergenic 50.0%
AGGTTAGAACGAGTGGCATC+AGG - chr3.1:74855557-74855576 None:intergenic 50.0%
AGGTTCCTTAATCAGTGCCC+CGG - chr3.1:74857026-74857045 None:intergenic 50.0%
GCCACTCGTTCTAACCTAAG+TGG + chr3.1:74855560-74855579 MS.gene06811:intron 50.0%
GGAGGATTAGAAGGCCATTC+TGG - chr3.1:74856006-74856025 None:intergenic 50.0%
TCGGGTCTGATGTAGCTTTC+GGG - chr3.1:74855228-74855247 None:intergenic 50.0%
! TCGGACAAGTGTCACTGACT+AGG + chr3.1:74856092-74856111 MS.gene06811:intron 50.0%
GGTGAAGCATGCAGCTCCTA+TGG + chr3.1:74855345-74855364 MS.gene06811:CDS 55.0%
GGTTCCTTAATCAGTGCCCC+GGG - chr3.1:74857025-74857044 None:intergenic 55.0%
GTTCCTTAATCAGTGCCCCG+GGG - chr3.1:74857024-74857043 None:intergenic 55.0%
TTCCTTAATCAGTGCCCCGG+GGG - chr3.1:74857023-74857042 None:intergenic 55.0%
GTCATGTTAACCGGTGCCCC+CGG + chr3.1:74857004-74857023 MS.gene06811:intron 60.0%
TCATGTTAACCGGTGCCCCC+GGG + chr3.1:74857005-74857024 MS.gene06811:intron 60.0%
CATGTTAACCGGTGCCCCCG+GGG + chr3.1:74857006-74857025 MS.gene06811:intron 65.0%
!! TGCCCCCGGGGCACTGATTA+AGG + chr3.1:74857018-74857037 MS.gene06811:intron 65.0%
! AATCAGTGCCCCGGGGGCAC+CGG - chr3.1:74857017-74857036 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr3.1 gene 74855180 74857543 74855180 ID=MS.gene06811
chr3.1 mRNA 74855180 74857543 74855180 ID=MS.gene06811.t1;Parent=MS.gene06811
chr3.1 exon 74855180 74855377 74855180 ID=MS.gene06811.t1.exon1;Parent=MS.gene06811.t1
chr3.1 CDS 74855180 74855377 74855180 ID=cds.MS.gene06811.t1;Parent=MS.gene06811.t1
chr3.1 exon 74855787 74855945 74855787 ID=MS.gene06811.t1.exon2;Parent=MS.gene06811.t1
chr3.1 CDS 74855787 74855945 74855787 ID=cds.MS.gene06811.t1;Parent=MS.gene06811.t1
chr3.1 exon 74857493 74857543 74857493 ID=MS.gene06811.t1.exon3;Parent=MS.gene06811.t1
chr3.1 CDS 74857493 74857543 74857493 ID=cds.MS.gene06811.t1;Parent=MS.gene06811.t1
Gene Sequence

>MS.gene06811

ATGGACACCAAAGTGCTTTCTTCCGGAATCCATCATTCAAAGTTACCCGAAAGCTACATCAGACCCGAATCTGATAGACCATGTCTTTCTCAAGTCTCTGAGTTCGAAAATGTTCCTATCATTGATTTGGGTTCTCACAACAGAACACAGATTGTGCAGCAAATTGGTGAAGCATGCAGCTCCTATGGTTTTTTCCAGGTCTAGCCCAATATTACAATTTTTTGTTTATTCATCATTATTCAATAACCATAGATGAACAATGAAGATTATTTTAGATAGGCATAAGTCATAACTTTTGCTATTTATTATAATTAGAATAGGATTAACTTAACTCCTCATTTCTACTAAATACAATTTGATTTGTCACATGCAAGCCTGATGCCACTCGTTCTAACCTAAGTGGTTGATGCGCAGTGTCTATGAGTCCAGAACCTAGTTCAATTTTGAGTGATTAAATAAAATGTCATTTTGGTAATTAAGTGACATTGTGAATACAGAGCTAATTGAATGACTGTGTAATGCATAGTCACTGAATTAATTTTATATTATATATTAAACACCGTAAGTTTTGGGAGACTAATTAATATATTGTTAATTAATTGGACAGGTAGTTAATCATGGGGTGCCTCTAGAAGAATTGAAAAAAACAGCAGAAGTGGCATATGATTTTTTCAAGTTGCCAGTGGAGGAGAAAATGAAGTTGTATTCAGACGATCCCACGAAGACAATGAGACTATCTACAAGTTTTAATGTGAACAAAGAGGAGGTGCATAATTGGAGGGACTACCTTAGACTTCATTGCTATCCTTTGGATAATTATGTGCCAGAATGGCCTTCTAATCCTCCTTCATTCAAGTAAGTCCACTCTCTTCATTTTTCTCTGTCTCTCTAATTTCAGCCACGTATCTATCATCGGACAAGTGTCACTGACTAGGTCTAGGCCTCACCTTGTGACATGTTTTATACATGTGATGATGTATTCATCATGAGGTCATTCCCTTGTTTATAAAAATAACTTGTGATGTAACATATGTAATGCATTTTTTTTAACTTTATGAAAATGATAAAAATGATGATAAATTACACTATGATCTTTTAAGTTTGAAGTTTGTAACATATTGATCATTTATATTATTTTGATTACACATAATTTTTTAAGTTTGAGGTTTATAATAGAGTGGTCCTTTAAGTTATTTGGATCACATATAAATCGTTTAAATTTGTAAACGTTTTTAATTTAGTAATTTTGTTAACCAAATATTACAAAAACGATGATGTGGCAGAATGACTAAGTCGAAAATATTTGAAAACTTAAAGGACTTATGTTTGATCAAAATAACTTAAAGACCAGTTACAAACCTCAAACTTAAAGGACTTATGTATGCTCAAAATAACTTAAAAATCAATTTGTAACAAATCTCAAATTTATAAGACACTTAGTGTAATTTAGAAAGAAATAAACGAGTCATGCTAATGAGCATGTTCAAGAAACCCTAAAAAAAAATTATCTTAAAAATATAAATTAAATACTTGTCAAAGTCATAAATACAAAGATTTTATGCAATATTTTTTTAAACAATGCCCTAAAAATATTTATGTTCTACTATTTTTTTTTATGATTTTTTAAAAAAGAGAAATGATATTTGTACAACCATTTTTTAACAACTTTTGTTACAACTTTATCTTTCATACTCACATTATGTTTTTGTTTTCTTTCTCTATTGCTTTGGTTTTTGTGTCAATATCCCAATTTCTTTGTAAAGTTATGGATGTGTCATAAGTTGTCTATCAAATGGTTGTTCAAATAACATAATTATTTAAAGGGTCATGTTAACCGGTGCCCCCGGGGCACTGATTAAGGAACCTAAAAAAGGAAATTTTAAAATAAAAATAACAGTTGTTATGTTTTTTTAGAAGTTGACAACACAAGTTCCAATGTAATACTTTCTACATTTTATTTCTTAACATTTCCCTTATTTAAAAAGTAAATTTTATTTTATTTGAATGTTAGTAGAACATAGATTTTTATTTTATTTTTTATTTATCGGTTTAGTGGCCACACATACTCAAATGAAAAATTTGTCTCCCCATATTCTTTTTGATTTATCTTTACATCATTTTAGCCTTTAGGCTGCATGCCCTTTAATTTCCACATTTTTTTACCAGGGAAATTAAATGGCATATACTTTTCGTAAAAAAGTATGGGGTCCTAGCTATATGTATACGAAATAGATAAAAGAGATACCGTTAGGTCATTTTCAGCTCCTATATTTATAATTATTTTTTTTTACCATCGATTTTTTTTACAAAAAGAATCTTATTCGATATGTGAAAGACCGGAAGACATTAAACGTGAAGAATTAA
Protein sequence

>MS.gene06811.t1

MDTKVLSSGIHHSKLPESYIRPESDRPCLSQVSEFENVPIIDLGSHNRTQIVQQIGEACSSYGFFQVVNHGVPLEELKKTAEVAYDFFKLPVEEKMKLYSDDPTKTMRLSTSFNVNKEENLIRYVKDRKTLNVKN