AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene068226

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene068226.t1 MTR_1g048950 88.382 482 32 1 1 482 77 534 0.0 843
MS.gene068226.t1 MTR_5g013420 40.467 514 225 10 1 481 624 1089 7.03e-108 345
MS.gene068226.t1 MTR_5g013420 40.467 514 225 10 1 481 624 1089 4.56e-107 343
MS.gene068226.t1 MTR_4g010830 39.689 514 228 11 1 481 516 980 3.72e-103 330
MS.gene068226.t1 MTR_4g010550 38.618 492 218 11 9 481 38 464 5.00e-97 300
MS.gene068226.t1 MTR_3g011440 33.012 518 256 15 9 480 191 663 2.23e-72 241
MS.gene068226.t1 MTR_7g088370 32.475 505 267 15 1 478 245 702 6.19e-71 239
MS.gene068226.t1 MTR_6g061200 33.130 492 267 13 11 481 215 665 1.24e-70 237
MS.gene068226.t1 MTR_8g042750 31.907 514 260 10 9 478 218 685 3.75e-70 236
MS.gene068226.t1 MTR_6g061270 32.500 520 262 13 11 481 215 694 9.16e-70 235
MS.gene068226.t1 MTR_7g084090 31.870 524 259 14 12 481 278 757 2.01e-69 236
MS.gene068226.t1 MTR_1g035420 32.787 488 254 12 1 455 198 644 3.95e-63 217
MS.gene068226.t1 MTR_8g070075 30.816 490 264 14 1 455 299 748 1.09e-61 214
MS.gene068226.t1 MTR_7g445710 30.435 529 219 13 1 481 116 543 1.89e-61 210
MS.gene068226.t1 MTR_8g070070 32.041 490 258 13 1 455 306 755 2.67e-61 214
MS.gene068226.t1 MTR_7g450730 31.537 501 215 12 1 481 113 505 3.11e-61 208
MS.gene068226.t1 MTR_8g071030 32.587 491 254 15 1 455 286 735 5.82e-61 213
MS.gene068226.t1 MTR_7g445530 30.862 499 201 13 1 456 116 513 5.31e-57 197
MS.gene068226.t1 MTR_6g044690 31.884 483 240 15 12 455 130 562 3.36e-56 196
MS.gene068226.t1 MTR_1g035400 31.034 493 258 16 1 455 115 563 2.89e-52 186
MS.gene068226.t1 MTR_7g084090 32.713 376 191 9 12 369 278 609 4.56e-52 187
MS.gene068226.t1 MTR_6g043960 29.193 483 232 16 12 455 69 480 1.58e-46 168
MS.gene068226.t1 MTR_0376s0030 27.879 495 275 14 12 471 130 577 1.63e-45 167
MS.gene068226.t1 MTR_2g054460 28.992 476 214 15 12 455 79 462 2.17e-42 157
MS.gene068226.t1 MTR_7g052480 31.699 306 167 8 214 481 255 556 1.67e-37 145
MS.gene068226.t1 MTR_5g018850 31.778 343 178 10 192 481 1168 1507 1.11e-34 139
MS.gene068226.t1 MTR_8g078490 30.581 327 171 10 201 481 937 1253 2.05e-34 138
MS.gene068226.t1 MTR_7g445600 46.512 129 57 2 328 456 240 356 4.65e-34 131
MS.gene068226.t1 MTR_7g445600 36.000 125 70 3 1 124 112 227 4.12e-13 70.9
MS.gene068226.t1 MTR_8g078505 32.313 294 154 9 223 476 245 533 7.06e-33 131
MS.gene068226.t1 MTR_4g132610 36.816 201 106 5 297 481 852 1047 2.24e-30 125
MS.gene068226.t1 MTR_1g069570 29.299 314 157 10 228 476 428 741 2.78e-26 113
MS.gene068226.t1 MTR_1g080340 29.693 293 140 10 228 455 267 558 3.38e-25 109
MS.gene068226.t1 MTR_7g098390 27.077 325 160 11 222 476 380 697 1.34e-19 92.4
MS.gene068226.t1 MTR_1g069570 28.514 249 116 8 228 414 428 676 4.17e-19 90.9
MS.gene068226.t1 MTR_1g098000 36.000 175 84 7 316 477 72 231 9.67e-19 89.4
MS.gene068226.t1 MTR_1g098370 35.429 175 85 7 316 477 75 234 4.37e-18 87.0
MS.gene068226.t1 MTR_6g027690 29.075 227 123 8 271 480 214 419 1.69e-17 85.1
MS.gene068226.t1 MTR_3g088625 32.530 166 100 4 320 480 1200 1358 4.38e-16 82.0
MS.gene068226.t1 MTR_3g088625 32.530 166 100 4 320 480 1306 1464 5.64e-16 81.6
MS.gene068226.t1 MTR_0071s0100 33.939 165 97 5 317 478 191 346 1.83e-15 78.6
MS.gene068226.t1 MTR_1g012910 35.862 145 79 5 340 476 528 666 3.38e-14 75.5
MS.gene068226.t1 MTR_1g012910 35.862 145 79 5 340 476 365 503 4.06e-14 75.1
MS.gene068226.t1 MTR_6g059310 28.814 177 104 5 313 480 87 250 6.43e-14 73.2
MS.gene068226.t1 MTR_6g059310 28.814 177 104 5 313 480 87 250 8.20e-14 73.2
MS.gene068226.t1 MTR_1g012890 33.793 145 82 5 340 476 225 363 1.90e-12 68.9
MS.gene068226.t1 MTR_7g445810 33.571 140 80 4 12 151 141 267 7.35e-11 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene068226.t1 AT2G35160 42.977 477 217 14 14 481 328 758 4.30e-109 340
MS.gene068226.t1 AT2G35160 42.977 477 217 14 14 481 364 794 1.73e-108 340
MS.gene068226.t1 AT2G35160 42.977 477 217 14 14 481 364 794 1.73e-108 340
MS.gene068226.t1 AT2G35160 42.977 477 217 14 14 481 364 794 1.73e-108 340
MS.gene068226.t1 AT2G35160 42.977 477 217 14 14 481 364 794 1.73e-108 340
MS.gene068226.t1 AT2G22740 36.641 524 241 12 1 481 315 790 1.14e-91 296
MS.gene068226.t1 AT2G22740 36.641 524 241 12 1 481 315 790 1.14e-91 296
MS.gene068226.t1 AT1G73100 32.685 514 257 14 9 480 202 668 4.32e-71 238
MS.gene068226.t1 AT5G04940 33.008 512 256 14 9 480 205 669 2.66e-69 234
MS.gene068226.t1 AT5G04940 33.008 512 256 14 9 480 205 669 2.66e-69 234
MS.gene068226.t1 AT5G13960 31.407 519 267 16 12 481 12 490 1.32e-62 211
MS.gene068226.t1 AT2G33290 32.673 505 260 15 1 471 192 650 3.11e-62 214
MS.gene068226.t1 AT2G33290 32.673 505 260 15 1 471 192 650 3.11e-62 214
MS.gene068226.t1 AT2G33290 32.673 505 260 15 1 471 192 650 3.11e-62 214
MS.gene068226.t1 AT2G33290 32.673 505 260 15 1 471 192 650 3.11e-62 214
MS.gene068226.t1 AT5G13960 31.395 516 271 16 12 481 146 624 1.59e-61 212
MS.gene068226.t1 AT4G13460 30.658 486 255 12 7 455 196 636 2.34e-58 204
MS.gene068226.t1 AT4G13460 30.658 486 255 12 7 455 196 636 2.34e-58 204
MS.gene068226.t1 AT1G17770 30.385 520 266 18 9 480 221 692 6.67e-56 198
MS.gene068226.t1 AT2G24740 30.059 509 261 16 9 480 304 754 9.97e-52 187
MS.gene068226.t1 AT2G23740 33.758 314 162 11 204 478 1073 1379 3.68e-35 140
MS.gene068226.t1 AT2G23740 33.758 314 162 11 204 478 1073 1379 3.68e-35 140
MS.gene068226.t1 AT2G23740 33.758 314 162 11 204 478 1073 1379 3.68e-35 140
MS.gene068226.t1 AT2G23740 33.758 314 162 11 204 478 1066 1372 3.81e-35 140
MS.gene068226.t1 AT5G47150 44.512 164 81 3 9 171 170 324 7.43e-29 116
MS.gene068226.t1 AT5G47160 43.030 165 75 3 9 167 256 407 2.68e-27 114
MS.gene068226.t1 AT5G47160 43.030 165 75 3 9 167 256 407 2.68e-27 114
MS.gene068226.t1 AT5G47160 43.030 165 75 3 9 167 256 407 2.68e-27 114
MS.gene068226.t1 AT3G04380 26.744 430 213 19 125 476 52 457 8.31e-24 104
MS.gene068226.t1 AT3G04380 26.744 430 213 19 125 476 36 441 8.58e-24 104
MS.gene068226.t1 AT3G04380 26.744 430 213 19 125 476 52 457 1.05e-23 104
MS.gene068226.t1 AT3G04380 28.274 336 172 13 211 479 84 417 4.78e-23 102
MS.gene068226.t1 AT3G04380 28.529 333 169 13 211 476 100 430 5.94e-23 101
MS.gene068226.t1 AT3G04380 28.529 333 169 13 211 476 100 430 6.41e-23 101
MS.gene068226.t1 AT2G23740 32.932 249 122 10 204 414 1073 1314 1.19e-22 102
MS.gene068226.t1 AT2G23740 32.932 249 122 10 204 414 1073 1314 1.19e-22 102
MS.gene068226.t1 AT3G03750 33.696 184 100 4 316 480 169 349 6.85e-21 94.4
MS.gene068226.t1 AT5G43990 34.973 183 104 7 308 476 523 704 1.19e-20 95.9
MS.gene068226.t1 AT5G43990 34.973 183 104 7 308 476 523 704 1.19e-20 95.9
MS.gene068226.t1 AT5G43990 34.973 183 104 7 308 476 520 701 1.22e-20 95.9
MS.gene068226.t1 AT5G43990 34.973 183 104 7 308 476 520 701 1.22e-20 95.9
MS.gene068226.t1 AT5G43990 34.973 183 104 7 308 476 520 701 1.22e-20 95.9
MS.gene068226.t1 AT5G43990 34.973 183 104 7 308 476 520 701 1.22e-20 95.9
MS.gene068226.t1 AT5G43990 34.973 183 104 7 308 476 500 681 1.23e-20 95.9
MS.gene068226.t1 AT5G43990 34.973 183 104 7 308 476 500 681 1.23e-20 95.9
MS.gene068226.t1 AT5G43990 34.973 183 104 7 308 476 500 681 1.23e-20 95.9
MS.gene068226.t1 AT5G43990 34.973 183 104 7 308 476 500 681 1.23e-20 95.9
MS.gene068226.t1 AT5G43990 34.973 183 104 7 308 476 431 612 1.34e-20 95.5
MS.gene068226.t1 AT5G43990 34.973 183 104 7 308 476 543 724 1.42e-20 95.5
MS.gene068226.t1 AT5G43990 34.973 183 104 7 308 476 543 724 1.42e-20 95.5
MS.gene068226.t1 AT5G43990 34.973 183 104 7 308 476 451 632 1.42e-20 95.5
MS.gene068226.t1 AT5G43990 34.973 183 104 7 308 476 451 632 1.42e-20 95.5
MS.gene068226.t1 AT5G43990 34.973 183 104 7 308 476 451 632 1.42e-20 95.5
MS.gene068226.t1 AT5G43990 34.973 183 104 7 308 476 451 632 1.42e-20 95.5
MS.gene068226.t1 AT1G04050 31.915 188 110 5 308 479 536 721 8.11e-19 90.1
MS.gene068226.t1 AT1G04050 31.915 188 110 5 308 479 536 721 8.11e-19 90.1
MS.gene068226.t1 AT1G04050 31.915 188 110 5 308 479 432 617 9.41e-19 89.7
MS.gene068226.t1 AT1G77300 30.488 164 106 3 320 480 1008 1166 1.26e-15 80.5
MS.gene068226.t1 AT1G77300 30.952 168 100 4 320 480 1008 1166 1.38e-15 80.5
MS.gene068226.t1 AT3G03750 33.728 169 104 4 316 480 169 333 1.94e-15 77.8
MS.gene068226.t1 AT4G30860 28.631 241 130 8 245 480 261 464 1.02e-13 73.6
MS.gene068226.t1 AT1G76710 31.395 172 98 7 316 478 66 226 3.00e-13 72.4
MS.gene068226.t1 AT1G76710 31.395 172 98 7 316 478 66 226 3.08e-13 72.0
MS.gene068226.t1 AT1G76710 31.395 172 98 7 316 478 66 226 3.08e-13 72.0
MS.gene068226.t1 AT1G76710 31.395 172 98 7 316 478 66 226 3.09e-13 72.4
MS.gene068226.t1 AT3G59960 30.539 167 94 4 323 480 96 249 4.72e-11 63.9
MS.gene068226.t1 AT3G59960 30.539 167 94 4 323 480 96 249 4.72e-11 63.9
MS.gene068226.t1 AT3G59960 30.539 167 94 4 323 480 96 249 4.72e-11 63.9
MS.gene068226.t1 AT5G42400 30.201 149 86 5 339 480 1250 1387 6.53e-11 65.5
MS.gene068226.t1 AT5G42400 30.201 149 86 5 339 480 1250 1387 6.53e-11 65.5
MS.gene068226.t1 AT5G42400 30.201 149 86 5 339 480 1250 1387 6.53e-11 65.5
MS.gene068226.t1 AT5G42400 30.201 149 86 5 339 480 1250 1387 6.53e-11 65.5
MS.gene068226.t1 AT5G42400 30.201 149 86 5 339 480 1250 1387 6.53e-11 65.5
MS.gene068226.t1 AT5G42400 30.201 149 86 5 339 480 1250 1387 6.53e-11 65.5
MS.gene068226.t1 AT5G42400 30.201 149 86 5 339 480 1250 1387 6.53e-11 65.5
MS.gene068226.t1 AT5G42400 30.201 149 86 5 339 480 1250 1387 6.53e-11 65.5
MS.gene068226.t1 AT3G59960 30.539 167 94 4 323 480 96 249 9.40e-11 63.5

Find 121 sgRNAs with CRISPR-Local

Find 136 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTCATGTGGTATCGATTATT+TGG 0.102559 1.2:-32532725 MS.gene068226:CDS
TACATGCCCTATTCTCTTTC+CGG 0.210201 1.2:+32532819 None:intergenic
TGTTTCCCCTCGAGATGTTT+TGG 0.240459 1.2:-32532326 MS.gene068226:CDS
GCGACACCGTCCCTTGTATT+AGG 0.249242 1.2:+32532598 None:intergenic
CTTCAGAATTGTGTCTCTTC+TGG 0.280628 1.2:+32532296 None:intergenic
GGTATCTCCACATAGCTTAT+AGG 0.280802 1.2:+32532115 None:intergenic
TCCTGTTAGGGTTATATTGC+AGG 0.288637 1.2:-32532512 MS.gene068226:CDS
GTTTGTGCTTAAGAAAATTC+AGG 0.305686 1.2:-32532362 MS.gene068226:CDS
GTTCGGCCCTATATTGAAAT+TGG 0.307938 1.2:+32532776 None:intergenic
AGTATTGAACGATTCGTCTT+TGG 0.310118 1.2:+32532058 None:intergenic
GTATCGATTATTTGGGTAAA+GGG 0.327727 1.2:-32532717 MS.gene068226:CDS
TGTTCGAATCAATGATATTT+CGG 0.328396 1.2:-32532173 MS.gene068226:CDS
ACTAGGTAACCGAAGGGTTT+AGG 0.329178 1.2:+32532094 None:intergenic
CGCGCCTCATGATCAGAAAT+TGG 0.347176 1.2:-32532578 MS.gene068226:CDS
ACTCGAGTAAGAGGACAATT+TGG 0.352436 1.2:-32532400 MS.gene068226:CDS
TGGTGACCAATTTCAATATA+GGG 0.354159 1.2:-32532782 MS.gene068226:CDS
CGCGCGGAAATCTTGCTTTG+TGG 0.366478 1.2:-32532555 MS.gene068226:CDS
ATATAAAGGATGATTTCATT+TGG 0.370033 1.2:+32531927 None:intergenic
ATAGGTGTCACCACCCGAAT+AGG 0.370547 1.2:+32532133 None:intergenic
TATAAAGGATGATTTCATTT+GGG 0.376053 1.2:+32531928 None:intergenic
ACCTGCAATATAACCCTAAC+AGG 0.380684 1.2:+32532511 None:intergenic
GGGTAAAGGGGAGGGTTCTT+TGG 0.380696 1.2:-32532704 MS.gene068226:CDS
TCTGGTTGTTTGCTTTATGA+AGG 0.381509 1.2:-32532634 MS.gene068226:CDS
AATTTGCTACTGTGGTTCAT+TGG 0.383046 1.2:-32531358 MS.gene068226:CDS
TGAATGCGGGGAGTCCTGTT+AGG 0.386175 1.2:-32532525 MS.gene068226:CDS
GCAAAGAAAAGTTCCCTATT+CGG 0.386328 1.2:-32532147 MS.gene068226:CDS
CGACACCGTCCCTTGTATTA+GGG 0.401197 1.2:+32532599 None:intergenic
TCTTATTCATACATATATGA+TGG 0.403540 1.2:-32532445 MS.gene068226:CDS
TTGGTGACCAATTTCAATAT+AGG 0.405625 1.2:-32532783 MS.gene068226:CDS
CAAAGAAAAGTTCCCTATTC+GGG 0.406699 1.2:-32532146 MS.gene068226:CDS
GCGAATGTTATGAAGTCTTC+TGG 0.411419 1.2:-32532652 MS.gene068226:CDS
GATGAGTATACAATTAATAT+AGG 0.418381 1.2:-32531692 MS.gene068226:intron
AGGGCCACACTCATATATAA+AGG 0.421860 1.2:+32531913 None:intergenic
TCATGTGGTATCGATTATTT+GGG 0.431263 1.2:-32532724 MS.gene068226:CDS
AAGACGAATCGTTCAATACT+AGG 0.431282 1.2:-32532055 MS.gene068226:CDS
ACATCTTTCACAACTAGGTT+AGG 0.431941 1.2:+32531507 None:intergenic
CAAAATGTTGTTTGCTACAA+AGG 0.440548 1.2:-32531451 MS.gene068226:CDS
GAGCTCACACACAAAGCTTC+CGG 0.447492 1.2:+32531769 None:intergenic
GAAAACACCGGAAAGAGAAT+AGG 0.449177 1.2:-32532826 MS.gene068226:CDS
AGGGCCGAACTTATTGTAAT+CGG 0.452930 1.2:-32532763 MS.gene068226:CDS
AAAACACCGGAAAGAGAATA+GGG 0.465297 1.2:-32532825 MS.gene068226:CDS
GATTATTTGGGTAAAGGGGA+GGG 0.467741 1.2:-32532712 MS.gene068226:CDS
AATCTTCAAAACAAAATTGA+AGG 0.468829 1.2:-32531825 MS.gene068226:CDS
AAGAGAATAGGGCATGTAGA+TGG 0.472138 1.2:-32532814 MS.gene068226:CDS
GGTATCGATTATTTGGGTAA+AGG 0.478346 1.2:-32532718 MS.gene068226:CDS
GACAATGTGACTCGAGTAAG+AGG 0.478502 1.2:-32532409 MS.gene068226:CDS
TTATGAAGGCGAAACGTCAA+TGG 0.484134 1.2:-32532849 MS.gene068226:CDS
TTCAAGTTGGAACTGAATAC+CGG 0.487311 1.2:+32531847 None:intergenic
TAGGTGTCACCACCCGAATA+GGG 0.488457 1.2:+32532134 None:intergenic
TACCACATGAGTGTTGTTGA+TGG 0.494297 1.2:+32532737 None:intergenic
TAAGAAAATTCAGGACCAAC+CGG 0.497017 1.2:-32532353 MS.gene068226:CDS
CTTATTCATACATATATGAT+GGG 0.498680 1.2:-32532444 MS.gene068226:CDS
ATTGATTCGAACAACATCCT+TGG 0.502629 1.2:+32532183 None:intergenic
CGATTATTTGGGTAAAGGGG+AGG 0.503266 1.2:-32532713 MS.gene068226:CDS
AGCTTTGTGTGTGAGCTCAT+TGG 0.503294 1.2:-32531764 MS.gene068226:CDS
TTCATTGATGCAACGCGACA+TGG 0.503420 1.2:-32531564 MS.gene068226:CDS
ACATAACATCTTTCACAACT+AGG 0.508844 1.2:+32531502 None:intergenic
CAAAACAAAATTGAAGGGGT+GGG 0.513316 1.2:-32531819 MS.gene068226:CDS
ATGGACAAATCTACATTTGA+AGG 0.517049 1.2:-32531329 None:intergenic
ACGAGGAACACAAGCAAGAA+GGG 0.521209 1.2:+32531894 None:intergenic
ATCAGCCGAGTGAGTCAATC+CGG 0.522206 1.2:-32531866 MS.gene068226:CDS
GACGAGGAACACAAGCAAGA+AGG 0.525389 1.2:+32531893 None:intergenic
ACACGCTCTTTCATTCCATC+CGG 0.526296 1.2:-32531788 MS.gene068226:CDS
AAACATTCTTTGGGAGAACA+TGG 0.531170 1.2:+32532272 None:intergenic
CATGTAGATGGAATTGAAGT+TGG 0.532327 1.2:-32532802 MS.gene068226:CDS
TTGAGTAAAATTTGCTACTG+TGG 0.533623 1.2:-32531366 MS.gene068226:CDS
GAATGCGGGGAGTCCTGTTA+GGG 0.541817 1.2:-32532524 MS.gene068226:CDS
GAGACCGATTACAATAAGTT+CGG 0.543201 1.2:+32532759 None:intergenic
GCAACGCGACATGGAAACAT+TGG 0.543624 1.2:-32531555 MS.gene068226:CDS
TCAAAACAAAATTGAAGGGG+TGG 0.543766 1.2:-32531820 MS.gene068226:CDS
ATCTTCAAAACAAAATTGAA+GGG 0.544882 1.2:-32531824 MS.gene068226:CDS
CGCGCCAATTTCTGATCATG+AGG 0.548143 1.2:+32532574 None:intergenic
TATGAAGGCGAAACGTCAAT+GGG 0.552062 1.2:-32532848 MS.gene068226:CDS
AAAACAAAATTGAAGGGGTG+GGG 0.557230 1.2:-32531818 MS.gene068226:CDS
TAGAGGTCTTGTTCTCTCCA+AGG 0.558562 1.2:-32532200 MS.gene068226:CDS
TCTATGAGTTACAACAATGC+TGG 0.561265 1.2:+32532678 None:intergenic
TTTGCATAGCTAAGAATGGC+GGG 0.567635 1.2:-32531985 MS.gene068226:CDS
GTGGAGATACCTAAACCCTT+CGG 0.568688 1.2:-32532103 MS.gene068226:CDS
GGCGGGACATTAGCCTATGA+TGG 0.570158 1.2:-32531968 MS.gene068226:CDS
CGCGACATGGAAACATTGGA+AGG 0.575552 1.2:-32531551 MS.gene068226:CDS
TGTTTGCTTTATGAAGGTCA+TGG 0.576682 1.2:-32532628 MS.gene068226:CDS
TTGGTTTCCATAACATCTCG+AGG 0.577098 1.2:+32532247 None:intergenic
CGAATCAATGATATTTCGGA+GGG 0.577253 1.2:-32532169 MS.gene068226:CDS
ATGGTGGTAACCCTAATACA+AGG 0.577358 1.2:-32532609 MS.gene068226:CDS
CAGAAGAGACACAATTCTGA+AGG 0.581158 1.2:-32532295 MS.gene068226:CDS
TTGCTGCAAGGTGAACATCA+AGG 0.582980 1.2:-32531391 MS.gene068226:CDS
TAACTCAGCTCTCTACCTGC+GGG 0.583390 1.2:+32531423 None:intergenic
AATCGTTCAATACTAGGTGG+TGG 0.591568 1.2:-32532049 MS.gene068226:CDS
TTGTGGAATAGTATGAATGC+GGG 0.595742 1.2:-32532538 MS.gene068226:CDS
CATATTGACTAGGTAACCGA+AGG 0.601280 1.2:+32532087 None:intergenic
AAAGTTCCCAAAACATCTCG+AGG 0.611731 1.2:+32532319 None:intergenic
TCTCGAGGGGAAACACTAGC+CGG 0.615076 1.2:+32532334 None:intergenic
TGTGGTTCATTGGAATGCAA+TGG 0.618637 1.2:-32531348 MS.gene068226:CDS
TTGGATAAGTCATATTGACT+AGG 0.621126 1.2:+32532077 None:intergenic
TGTGTTTGCATAGCTAAGAA+TGG 0.625244 1.2:-32531989 MS.gene068226:CDS
TATCGATTATTTGGGTAAAG+GGG 0.626149 1.2:-32532716 MS.gene068226:CDS
TCTTCAAAACAAAATTGAAG+GGG 0.626316 1.2:-32531823 MS.gene068226:CDS
TCGAATCAATGATATTTCGG+AGG 0.627900 1.2:-32532170 MS.gene068226:CDS
AAGTTCCCAAAACATCTCGA+GGG 0.630440 1.2:+32532320 None:intergenic
GAGGGGAAACACTAGCCGGT+TGG 0.631704 1.2:+32532338 None:intergenic
ATAACTCAGCTCTCTACCTG+CGG 0.634593 1.2:+32531422 None:intergenic
CACTCGGCTGATGCAAGACG+AGG 0.637041 1.2:+32531877 None:intergenic
TCATCCTTTATATATGAGTG+TGG 0.641509 1.2:-32531917 MS.gene068226:CDS
GGTGACACCTATAAGCTATG+TGG 0.646281 1.2:-32532122 MS.gene068226:CDS
GGTAACCCTAATACAAGGGA+CGG 0.650433 1.2:-32532604 MS.gene068226:CDS
GCTACAAAGGACATACCCGC+AGG 0.652394 1.2:-32531438 MS.gene068226:CDS
TGGTGGTAACCCTAATACAA+GGG 0.655937 1.2:-32532608 MS.gene068226:CDS
CTCCATCAACAACACTCATG+TGG 0.665071 1.2:-32532739 MS.gene068226:CDS
AGTTCCCAAAACATCTCGAG+GGG 0.671999 1.2:+32532321 None:intergenic
GAATACCGGATTGACTCACT+CGG 0.672661 1.2:+32531861 None:intergenic
ATATTGACTAGGTAACCGAA+GGG 0.676746 1.2:+32532088 None:intergenic
TCACACACAAAGCTTCCGGA+TGG 0.681981 1.2:+32531773 None:intergenic
AGAAAAGTTCCCTATTCGGG+TGG 0.682829 1.2:-32532143 MS.gene068226:CDS
AAACGTCAATGGGAAAACAC+CGG 0.685237 1.2:-32532838 MS.gene068226:CDS
TGTGGAATAGTATGAATGCG+GGG 0.700340 1.2:-32532537 MS.gene068226:CDS
CATGATCAGAAATTGGCGCG+CGG 0.700635 1.2:-32532571 MS.gene068226:CDS
TTTGTGGAATAGTATGAATG+CGG 0.704358 1.2:-32532539 MS.gene068226:CDS
TTGCTTTATGAAGGTCATGG+TGG 0.708605 1.2:-32532625 MS.gene068226:CDS
GTTTGCATAGCTAAGAATGG+CGG 0.718623 1.2:-32531986 MS.gene068226:CDS
TTATGATTACAATTGCTGCA+AGG 0.726923 1.2:-32531403 MS.gene068226:CDS
ACGAATCGTTCAATACTAGG+TGG 0.736474 1.2:-32532052 MS.gene068226:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATCTTCAAAACAAAATTGA+AGG - chr1.2:32532354-32532373 MS.gene068226:CDS 20.0%
!! ATATAAAGGATGATTTCATT+TGG + chr1.2:32532255-32532274 None:intergenic 20.0%
!! ATCTTCAAAACAAAATTGAA+GGG - chr1.2:32532355-32532374 MS.gene068226:CDS 20.0%
!! CTTATTCATACATATATGAT+GGG - chr1.2:32531735-32531754 MS.gene068226:CDS 20.0%
!! GATGAGTATACAATTAATAT+AGG - chr1.2:32532487-32532506 MS.gene068226:CDS 20.0%
!! TATAAAGGATGATTTCATTT+GGG + chr1.2:32532254-32532273 None:intergenic 20.0%
!! TCTTATTCATACATATATGA+TGG - chr1.2:32531734-32531753 MS.gene068226:CDS 20.0%
! TCTTCAAAACAAAATTGAAG+GGG - chr1.2:32532356-32532375 MS.gene068226:CDS 25.0%
! TGTTCGAATCAATGATATTT+CGG - chr1.2:32532006-32532025 MS.gene068226:CDS 25.0%
!! TGATGTTTTATGCATTTTGT+AGG - chr1.2:32532576-32532595 MS.gene068226:CDS 25.0%
!!! TTGTTTTGAAGATTTCAAGT+TGG + chr1.2:32532348-32532367 None:intergenic 25.0%
ACATAACATCTTTCACAACT+AGG + chr1.2:32532680-32532699 None:intergenic 30.0%
CATCTCAACAACAAAAAAGT+TGG - chr1.2:32532448-32532467 MS.gene068226:CDS 30.0%
GTTTGTGCTTAAGAAAATTC+AGG - chr1.2:32531817-32531836 MS.gene068226:CDS 30.0%
TCATCCTTTATATATGAGTG+TGG - chr1.2:32532262-32532281 MS.gene068226:CDS 30.0%
TGGTGACCAATTTCAATATA+GGG - chr1.2:32531397-32531416 MS.gene068226:CDS 30.0%
TTATGATTACAATTGCTGCA+AGG - chr1.2:32532776-32532795 MS.gene068226:CDS 30.0%
TTTGTGGAATAGTATGAATG+CGG - chr1.2:32531640-32531659 MS.gene068226:intron 30.0%
! AAGTTTTCTGTTACCATCAT+AGG + chr1.2:32532227-32532246 None:intergenic 30.0%
! CAAAATGTTGTTTGCTACAA+AGG - chr1.2:32532728-32532747 MS.gene068226:CDS 30.0%
! CTCTAGACATTTGTCTTTTT+CGG + chr1.2:32531964-32531983 None:intergenic 30.0%
! TATCGATTATTTGGGTAAAG+GGG - chr1.2:32531463-32531482 MS.gene068226:CDS 30.0%
! TGTAGCAAACAACATTTTGT+GGG + chr1.2:32532727-32532746 None:intergenic 30.0%
! TTCTGAAAAATCTGAAGTGA+TGG - chr1.2:32531691-32531710 MS.gene068226:intron 30.0%
! TTGGATAAGTCATATTGACT+AGG + chr1.2:32532105-32532124 None:intergenic 30.0%
! TTGGTGACCAATTTCAATAT+AGG - chr1.2:32531396-32531415 MS.gene068226:CDS 30.0%
! TTGTAGCAAACAACATTTTG+TGG + chr1.2:32532728-32532747 None:intergenic 30.0%
!! GTATCGATTATTTGGGTAAA+GGG - chr1.2:32531462-32531481 MS.gene068226:CDS 30.0%
!! TCATGTGGTATCGATTATTT+GGG - chr1.2:32531455-32531474 MS.gene068226:CDS 30.0%
!! TTGAGTAAAATTTGCTACTG+TGG - chr1.2:32532813-32532832 MS.gene068226:CDS 30.0%
!!! ATATGATGGGTTGTATTTTG+TGG - chr1.2:32531748-32531767 MS.gene068226:CDS 30.0%
!!! TTGTCTTTTTCGGAAAGTTT+TGG + chr1.2:32531954-32531973 None:intergenic 30.0%
AAAACAAAATTGAAGGGGTG+GGG - chr1.2:32532361-32532380 MS.gene068226:CDS 35.0%
AAAACACCGGAAAGAGAATA+GGG - chr1.2:32531354-32531373 MS.gene068226:CDS 35.0%
AAGACGAATCGTTCAATACT+AGG - chr1.2:32532124-32532143 MS.gene068226:CDS 35.0%
ACATCTTTCACAACTAGGTT+AGG + chr1.2:32532675-32532694 None:intergenic 35.0%
CAAAACAAAATTGAAGGGGT+GGG - chr1.2:32532360-32532379 MS.gene068226:CDS 35.0%
CAAAGAAAAGTTCCCTATTC+GGG - chr1.2:32532033-32532052 MS.gene068226:CDS 35.0%
CATGTAGATGGAATTGAAGT+TGG - chr1.2:32531377-32531396 MS.gene068226:CDS 35.0%
CGAAAAAGACAAATGTCTAG+AGG - chr1.2:32531962-32531981 MS.gene068226:CDS 35.0%
CGAATCAATGATATTTCGGA+GGG - chr1.2:32532010-32532029 MS.gene068226:CDS 35.0%
CTCGAGGAAAAACATTCTTT+GGG + chr1.2:32531919-32531938 None:intergenic 35.0%
GAGACCGATTACAATAAGTT+CGG + chr1.2:32531423-32531442 None:intergenic 35.0%
GCAAAGAAAAGTTCCCTATT+CGG - chr1.2:32532032-32532051 MS.gene068226:CDS 35.0%
TAAGAAAATTCAGGACCAAC+CGG - chr1.2:32531826-32531845 MS.gene068226:CDS 35.0%
TCAAAACAAAATTGAAGGGG+TGG - chr1.2:32532359-32532378 MS.gene068226:CDS 35.0%
TCGAATCAATGATATTTCGG+AGG - chr1.2:32532009-32532028 MS.gene068226:CDS 35.0%
TCTATGAGTTACAACAATGC+TGG + chr1.2:32531504-32531523 None:intergenic 35.0%
TCTCGAGGAAAAACATTCTT+TGG + chr1.2:32531920-32531939 None:intergenic 35.0%
TCTGGTTGTTTGCTTTATGA+AGG - chr1.2:32531545-32531564 MS.gene068226:CDS 35.0%
TGTGTTTGCATAGCTAAGAA+TGG - chr1.2:32532190-32532209 MS.gene068226:CDS 35.0%
TGTTTGCTTTATGAAGGTCA+TGG - chr1.2:32531551-32531570 MS.gene068226:CDS 35.0%
TTGTGGAATAGTATGAATGC+GGG - chr1.2:32531641-32531660 MS.gene068226:intron 35.0%
! AAACATTCTTTGGGAGAACA+TGG + chr1.2:32531910-32531929 None:intergenic 35.0%
! AGTATTGAACGATTCGTCTT+TGG + chr1.2:32532124-32532143 None:intergenic 35.0%
! ATATTGACTAGGTAACCGAA+GGG + chr1.2:32532094-32532113 None:intergenic 35.0%
! GTAGCAAACAACATTTTGTG+GGG + chr1.2:32532726-32532745 None:intergenic 35.0%
! TGTTTTTCCTCGAGATGTTA+TGG - chr1.2:32531925-32531944 MS.gene068226:CDS 35.0%
!! AATTTGCTACTGTGGTTCAT+TGG - chr1.2:32532821-32532840 MS.gene068226:CDS 35.0%
!! ATTGATTCGAACAACATCCT+TGG + chr1.2:32531999-32532018 None:intergenic 35.0%
!! CTCATGTGGTATCGATTATT+TGG - chr1.2:32531454-32531473 MS.gene068226:CDS 35.0%
!! GGTATCGATTATTTGGGTAA+AGG - chr1.2:32531461-32531480 MS.gene068226:CDS 35.0%
!! TTCAAGTTGGAACTGAATAC+CGG + chr1.2:32532335-32532354 None:intergenic 35.0%
AAACGTCAATGGGAAAACAC+CGG - chr1.2:32531341-32531360 MS.gene068226:CDS 40.0%
AAAGTTCCCAAAACATCTCG+AGG + chr1.2:32531863-32531882 None:intergenic 40.0%
AAGAGAATAGGGCATGTAGA+TGG - chr1.2:32531365-32531384 MS.gene068226:CDS 40.0%
AAGTTCCCAAAACATCTCGA+GGG + chr1.2:32531862-32531881 None:intergenic 40.0%
AATCGTTCAATACTAGGTGG+TGG - chr1.2:32532130-32532149 MS.gene068226:CDS 40.0%
ACCTGCAATATAACCCTAAC+AGG + chr1.2:32531671-32531690 None:intergenic 40.0%
ACGAATCGTTCAATACTAGG+TGG - chr1.2:32532127-32532146 MS.gene068226:CDS 40.0%
ACTCGAGTAAGAGGACAATT+TGG - chr1.2:32531779-32531798 MS.gene068226:CDS 40.0%
AGGGCCACACTCATATATAA+AGG + chr1.2:32532269-32532288 None:intergenic 40.0%
AGGGCCGAACTTATTGTAAT+CGG - chr1.2:32531416-32531435 MS.gene068226:CDS 40.0%
ATGGTGGTAACCCTAATACA+AGG - chr1.2:32531570-32531589 MS.gene068226:CDS 40.0%
CAGAAGAGACACAATTCTGA+AGG - chr1.2:32531884-32531903 MS.gene068226:CDS 40.0%
CTTCAGAATTGTGTCTCTTC+TGG + chr1.2:32531886-32531905 None:intergenic 40.0%
GAAAACACCGGAAAGAGAAT+AGG - chr1.2:32531353-32531372 MS.gene068226:CDS 40.0%
GATTATTTGGGTAAAGGGGA+GGG - chr1.2:32531467-32531486 MS.gene068226:CDS 40.0%
GCGAATGTTATGAAGTCTTC+TGG - chr1.2:32531527-32531546 MS.gene068226:CDS 40.0%
GGTATCTCCACATAGCTTAT+AGG + chr1.2:32532067-32532086 None:intergenic 40.0%
GTTCGGCCCTATATTGAAAT+TGG + chr1.2:32531406-32531425 None:intergenic 40.0%
GTTTGCATAGCTAAGAATGG+CGG - chr1.2:32532193-32532212 MS.gene068226:CDS 40.0%
TACATGCCCTATTCTCTTTC+CGG + chr1.2:32531363-32531382 None:intergenic 40.0%
TCCTGTTAGGGTTATATTGC+AGG - chr1.2:32531667-32531686 MS.gene068226:intron 40.0%
TGGTGGTAACCCTAATACAA+GGG - chr1.2:32531571-32531590 MS.gene068226:CDS 40.0%
TGTGGAATAGTATGAATGCG+GGG - chr1.2:32531642-32531661 MS.gene068226:intron 40.0%
TGTGGTTCATTGGAATGCAA+TGG - chr1.2:32532831-32532850 MS.gene068226:CDS 40.0%
TTGCTTTATGAAGGTCATGG+TGG - chr1.2:32531554-32531573 MS.gene068226:CDS 40.0%
TTGGTTTCCATAACATCTCG+AGG + chr1.2:32531935-32531954 None:intergenic 40.0%
TTTGCATAGCTAAGAATGGC+GGG - chr1.2:32532194-32532213 MS.gene068226:CDS 40.0%
! ATGCATTTTGTAGGTGCTTC+TGG - chr1.2:32532585-32532604 MS.gene068226:CDS 40.0%
! CATATTGACTAGGTAACCGA+AGG + chr1.2:32532095-32532114 None:intergenic 40.0%
! TACCACATGAGTGTTGTTGA+TGG + chr1.2:32531445-32531464 None:intergenic 40.0%
ACACGCTCTTTCATTCCATC+CGG - chr1.2:32532391-32532410 MS.gene068226:CDS 45.0%
ACGAGGAACACAAGCAAGAA+GGG + chr1.2:32532288-32532307 None:intergenic 45.0%
ACTAGGTAACCGAAGGGTTT+AGG + chr1.2:32532088-32532107 None:intergenic 45.0%
AGAAAAGTTCCCTATTCGGG+TGG - chr1.2:32532036-32532055 MS.gene068226:CDS 45.0%
AGTTCCCAAAACATCTCGAG+GGG + chr1.2:32531861-32531880 None:intergenic 45.0%
ATAACTCAGCTCTCTACCTG+CGG + chr1.2:32532760-32532779 None:intergenic 45.0%
CGATTATTTGGGTAAAGGGG+AGG - chr1.2:32531466-32531485 MS.gene068226:CDS 45.0%
CTCCATCAACAACACTCATG+TGG - chr1.2:32531440-32531459 MS.gene068226:CDS 45.0%
GACAATGTGACTCGAGTAAG+AGG - chr1.2:32531770-32531789 MS.gene068226:CDS 45.0%
GGTAACCCTAATACAAGGGA+CGG - chr1.2:32531575-32531594 MS.gene068226:CDS 45.0%
GGTGACACCTATAAGCTATG+TGG - chr1.2:32532057-32532076 MS.gene068226:CDS 45.0%
GTGGAGATACCTAAACCCTT+CGG - chr1.2:32532076-32532095 MS.gene068226:CDS 45.0%
TTCATTGATGCAACGCGACA+TGG - chr1.2:32532615-32532634 MS.gene068226:CDS 45.0%
TTGCTGCAAGGTGAACATCA+AGG - chr1.2:32532788-32532807 MS.gene068226:CDS 45.0%
! AGCTTTGTGTGTGAGCTCAT+TGG - chr1.2:32532415-32532434 MS.gene068226:CDS 45.0%
! GAATACCGGATTGACTCACT+CGG + chr1.2:32532321-32532340 None:intergenic 45.0%
! GTTTCCCCTCGAGATGTTTT+GGG - chr1.2:32531854-32531873 MS.gene068226:CDS 45.0%
! TGTTTCCCCTCGAGATGTTT+TGG - chr1.2:32531853-32531872 MS.gene068226:CDS 45.0%
! TTTTGTAGGTGCTTCTGGCA+AGG - chr1.2:32532590-32532609 MS.gene068226:CDS 45.0%
!! TAGAGGTCTTGTTCTCTCCA+AGG - chr1.2:32531979-32531998 MS.gene068226:CDS 45.0%
ATCAGCCGAGTGAGTCAATC+CGG - chr1.2:32532313-32532332 MS.gene068226:CDS 50.0%
CATGATCAGAAATTGGCGCG+CGG - chr1.2:32531608-32531627 MS.gene068226:intron 50.0%
CGACACCGTCCCTTGTATTA+GGG + chr1.2:32531583-32531602 None:intergenic 50.0%
CGCGACATGGAAACATTGGA+AGG - chr1.2:32532628-32532647 MS.gene068226:CDS 50.0%
CGCGCCAATTTCTGATCATG+AGG + chr1.2:32531608-32531627 None:intergenic 50.0%
CGCGCCTCATGATCAGAAAT+TGG - chr1.2:32531601-32531620 MS.gene068226:intron 50.0%
GACGAGGAACACAAGCAAGA+AGG + chr1.2:32532289-32532308 None:intergenic 50.0%
GAGCTCACACACAAAGCTTC+CGG + chr1.2:32532413-32532432 None:intergenic 50.0%
GCAACGCGACATGGAAACAT+TGG - chr1.2:32532624-32532643 MS.gene068226:CDS 50.0%
TAACTCAGCTCTCTACCTGC+GGG + chr1.2:32532759-32532778 None:intergenic 50.0%
TCACACACAAAGCTTCCGGA+TGG + chr1.2:32532409-32532428 None:intergenic 50.0%
! ATAGGTGTCACCACCCGAAT+AGG + chr1.2:32532049-32532068 None:intergenic 50.0%
! TAGGTGTCACCACCCGAATA+GGG + chr1.2:32532048-32532067 None:intergenic 50.0%
CGCGCGGAAATCTTGCTTTG+TGG - chr1.2:32531624-32531643 MS.gene068226:intron 55.0%
GAATGCGGGGAGTCCTGTTA+GGG - chr1.2:32531655-32531674 MS.gene068226:intron 55.0%
GCGACACCGTCCCTTGTATT+AGG + chr1.2:32531584-32531603 None:intergenic 55.0%
GCTACAAAGGACATACCCGC+AGG - chr1.2:32532741-32532760 MS.gene068226:CDS 55.0%
GGGTAAAGGGGAGGGTTCTT+TGG - chr1.2:32531475-32531494 MS.gene068226:CDS 55.0%
TCTCGAGGGGAAACACTAGC+CGG + chr1.2:32531848-32531867 None:intergenic 55.0%
TGAATGCGGGGAGTCCTGTT+AGG - chr1.2:32531654-32531673 MS.gene068226:intron 55.0%
! GGCGGGACATTAGCCTATGA+TGG - chr1.2:32532211-32532230 MS.gene068226:CDS 55.0%
CACTCGGCTGATGCAAGACG+AGG + chr1.2:32532305-32532324 None:intergenic 60.0%
GAGGGGAAACACTAGCCGGT+TGG + chr1.2:32531844-32531863 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr1.2 gene 32531332 32532869 32531332 ID=MS.gene068226
chr1.2 mRNA 32531332 32532869 32531332 ID=MS.gene068226.t1;Parent=MS.gene068226
chr1.2 exon 32531693 32532869 32531693 ID=MS.gene068226.t1.exon1;Parent=MS.gene068226.t1
chr1.2 CDS 32531693 32532869 32531693 ID=cds.MS.gene068226.t1;Parent=MS.gene068226.t1
chr1.2 exon 32531332 32531603 32531332 ID=MS.gene068226.t1.exon2;Parent=MS.gene068226.t1
chr1.2 CDS 32531332 32531603 32531332 ID=cds.MS.gene068226.t1;Parent=MS.gene068226.t1
Gene Sequence

>MS.gene068226

ATGAAGGCGAAACGTCAATGGGAAAACACCGGAAAGAGAATAGGGCATGTAGATGGAATTGAAGTTGGTGACCAATTTCAATATAGGGCCGAACTTATTGTAATCGGTCTCCATCAACAACACTCATGTGGTATCGATTATTTGGGTAAAGGGGAGGGTTCTTTGGCAACCAGCATTGTTGTAACTCATAGATATGCGAATGTTATGAAGTCTTCTGGTTGTTTGCTTTATGAAGGTCATGGTGGTAACCCTAATACAAGGGACGGTGTCGCGCCTCATGATCAGAAATTGGCGCGCGGAAATCTTGCTTTGTGGAATAGTATGAATGCGGGGAGTCCTGTTAGGGTTATATTGCAGGTTTCTGAAAAATCTGAAGTGATGGAAGAGTTGAGCAACAATGTTTCTTATTCATACATATATGATGGGTTGTATTTTGTGGACAATGTGACTCGAGTAAGAGGACAATTTGGAAAACTTGTTTTCAAGTTTGTGCTTAAGAAAATTCAGGACCAACCGGCTAGTGTTTCCCCTCGAGATGTTTTGGGAACTTTCCAGAAGAGACACAATTCTGAAGGCCATGTTCTCCCAAAGAATGTTTTTCCTCGAGATGTTATGGAAACCAAAACTTTCCGAAAAAGACAAATGTCTAGAGGTCTTGTTCTCTCCAAGGATGTTGTTCGAATCAATGATATTTCGGAGGGCAAAGAAAAGTTCCCTATTCGGGTGGTGACACCTATAAGCTATGTGGAGATACCTAAACCCTTCGGTTACCTAGTCAATATGACTTATCCAAAGACGAATCGTTCAATACTAGGTGGTGGATGTAATTGTAAGAATGCATGTGTTGATTGTCTTGAATGTGTTTGCATAGCTAAGAATGGCGGGACATTAGCCTATGATGGTAACAGAAAACTTGTTTCCCAAATGAAATCATCCTTTATATATGAGTGTGGCCCTTCTTGCTTGTGTTCCTCGTCTTGCATCAGCCGAGTGAGTCAATCCGGTATTCAGTTCCAACTTGAAATCTTCAAAACAAAATTGAAGGGGTGGGGTGTTCGAACACGCTCTTTCATTCCATCCGGAAGCTTTGTGTGTGAGCTCATTGGAGAAGTTGTTCATCTCAACAACAAAAAAGTTGGATCAAATCTACATGTTGATGAGTATACAATTAATATAGGTAATGTTTTTCGTTGATGATGAATATTTAATATCTTTGTCAGTTTGTGTTTTGTACTTCATTTGATTGATGTTTTATGCATTTTGTAGGTGCTTCTGGCAAGGTCTTCATTGATGCAACGCGACATGGAAACATTGGAAGGTTTATAAATCATAGTTGTTCTCCTAACCTAGTTGTGAAAGATGTTATGTATGATCACAGTGATAAGAGTTTACCCCACAAAATGTTGTTTGCTACAAAGGACATACCCGCAGGTAGAGAGCTGAGTTATGATTACAATTGCTGCAAGGTGAACATCAAGGTTTTGAGTAAAATTTGCTACTGTGGTTCATTGGAATGCAATGGACAAATCTACATTTGA
Protein sequence

>MS.gene068226.t1

MKAKRQWENTGKRIGHVDGIEVGDQFQYRAELIVIGLHQQHSCGIDYLGKGEGSLATSIVVTHRYANVMKSSGCLLYEGHGGNPNTRDGVAPHDQKLARGNLALWNSMNAGSPVRVILQVSEKSEVMEELSNNVSYSYIYDGLYFVDNVTRVRGQFGKLVFKFVLKKIQDQPASVSPRDVLGTFQKRHNSEGHVLPKNVFPRDVMETKTFRKRQMSRGLVLSKDVVRINDISEGKEKFPIRVVTPISYVEIPKPFGYLVNMTYPKTNRSILGGGCNCKNACVDCLECVCIAKNGGTLAYDGNRKLVSQMKSSFIYECGPSCLCSSSCISRVSQSGIQFQLEIFKTKLKGWGVRTRSFIPSGSFVCELIGEVVHLNNKKVGSNLHVDEYTINIGASGKVFIDATRHGNIGRFINHSCSPNLVVKDVMYDHSDKSLPHKMLFATKDIPAGRELSYDYNCCKVNIKVLSKICYCGSLECNGQIYI