AlfalfaGEDB | MS.gene050020

M. sativa cultivar XinJiangDaYe / MS.gene050020

KEGG: -
GO: GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0097159 GO:1901363
PFAM: PF04770.15

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene050020.t1	XP_003615230.1	96.3	269	7	2	1	267	1	268	5.40E-74	287.7

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene050020.t1	Q9SB61	47.8	245	86	2	23	267	18	220	4.4e-40	166.4

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene050020.t1	G7KBF3	96.3	269	7	2	1	267	1	268	3.9e-74	287.7

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TFs/TRs:

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Gene ID	Type	Classification
MS.gene050020.t1	TF	zf-HD

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049159	MS.gene050020	0.818137	2.29E-52	-1.69E-46
MS.gene049255	MS.gene050020	0.816076	6.66E-52	-1.69E-46
MS.gene049260	MS.gene050020	0.819691	1.02E-52	-1.69E-46
MS.gene049341	MS.gene050020	0.845989	2.88E-59	-1.69E-46
MS.gene049382	MS.gene050020	0.804861	1.76E-49	-1.69E-46
MS.gene049404	MS.gene050020	0.803154	3.99E-49	-1.69E-46
MS.gene049435	MS.gene050020	0.801375	9.28E-49	-1.69E-46
MS.gene049821	MS.gene050020	0.845625	3.61E-59	-1.69E-46
MS.gene049850	MS.gene050020	0.817911	2.58E-52	-1.69E-46
MS.gene049979	MS.gene050020	0.817301	3.54E-52	-1.69E-46

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PPI:

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene050020.t1	MTR_5g065440	96.283	269	7	2	1	267	1	268	0.0	531
MS.gene050020.t1	MTR_3g100650	59.450	291	77	7	1	267	1	274	4.79e-95	281
MS.gene050020.t1	MTR_5g032600	51.542	227	76	8	49	267	72	272	2.34e-56	182
MS.gene050020.t1	MTR_4g134580	41.700	247	94	6	58	266	112	346	9.05e-51	170
MS.gene050020.t1	MTR_1g077020	41.509	212	92	5	56	266	6	186	1.06e-47	157
MS.gene050020.t1	MTR_2g076780	41.038	212	109	4	59	266	37	236	1.07e-46	156
MS.gene050020.t1	MTR_7g010300	40.724	221	98	6	56	266	85	282	2.09e-44	153
MS.gene050020.t1	MTR_5g020510	39.819	221	106	6	54	266	94	295	6.46e-44	150
MS.gene050020.t1	MTR_8g069080	42.326	215	103	7	58	266	68	267	1.48e-43	149
MS.gene050020.t1	MTR_6g012280	42.857	210	102	5	59	266	91	284	1.39e-40	143

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene050020.t1	AT4G24660	48.289	263	88	7	7	267	4	220	3.08e-65	203
MS.gene050020.t1	AT4G24660	47.893	261	84	8	7	263	4	216	2.97e-62	196
MS.gene050020.t1	AT2G02540	38.868	265	123	7	18	266	43	284	6.10e-58	187
MS.gene050020.t1	AT2G02540	38.868	265	123	7	18	266	43	284	6.10e-58	187
MS.gene050020.t1	AT1G14440	40.654	214	102	4	57	266	88	280	9.77e-52	171
MS.gene050020.t1	AT1G14440	40.654	214	102	4	57	266	88	280	9.77e-52	171
MS.gene050020.t1	AT5G65410	43.519	216	87	3	55	267	71	254	3.40e-49	164
MS.gene050020.t1	AT2G18350	41.981	212	90	4	57	266	80	260	2.50e-47	158
MS.gene050020.t1	AT1G75240	40.693	231	118	2	56	267	73	303	8.34e-47	159
MS.gene050020.t1	AT3G50890	40.566	212	94	3	58	265	59	242	1.92e-43	148

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Find 79 sgRNAs with CRISPR-Local

Find 84 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACGGTTAGTTCATGTAAC+CGG	0.491216	5.4:+51440772	MS.gene050020:CDS
AAACTCACCGTTGGAGTTGA+CGG	0.483943	5.4:+51440546	MS.gene050020:CDS
AACCCTAGCAGTAGCGGTGG+AGG	0.520715	5.4:+51440993	MS.gene050020:CDS
AACGGTCTGTGATGTGCCAC+CGG	0.497928	5.4:-51440912	None:intergenic
AAGAATCACGCCGTTAGCTT+CGG	0.428386	5.4:+51440627	MS.gene050020:CDS
AAGGCGAGAATTGATTGCTG+TGG	0.722344	5.4:-51440830	None:intergenic
AATCACGCCGTTAGCTTCGG+TGG	0.536570	5.4:+51440630	MS.gene050020:CDS
AATGGAATTTGATGAACAAG+AGG	0.584250	5.4:+51440437	None:intergenic
AGAATTGATTGCTGTGGTGA+TGG	0.582941	5.4:-51440824	None:intergenic
AGAGAAATGTTCTTAAAGTT+TGG	0.256503	5.4:+51441180	MS.gene050020:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ATTCAGAACAAAATTCACTC+AGG	+	chr5.4:51441055-51441074	MS.gene050020:CDS	30.0%
CACAATAACAAGAACACTCT+TGG	+	chr5.4:51441206-51441225	MS.gene050020:CDS	35.0%
AGAATTGATTGCTGTGGTGA+TGG	-	chr5.4:51440827-51440846	None:intergenic	40.0%
CGTGATTCTTCTGACATTCT+CGG	-	chr5.4:51440617-51440636	None:intergenic	40.0%
TGAACAAGAGGAACAAGAAG+AGG	+	chr5.4:51440449-51440468	MS.gene050020:CDS	40.0%
TTCAAGTGTTCCTTCTTCAC+CGG	-	chr5.4:51440691-51440710	None:intergenic	40.0%
AAACTCACCGTTGGAGTTGA+CGG	+	chr5.4:51440546-51440565	MS.gene050020:CDS	45.0%
AAGGCGAGAATTGATTGCTG+TGG	-	chr5.4:51440833-51440852	None:intergenic	45.0%
AGAGGAACAAGAAGAGGAGA+TGG	+	chr5.4:51440455-51440474	MS.gene050020:CDS	45.0%
ATCTCAAACCCTAGCAGTAG+CGG	+	chr5.4:51440987-51441006	MS.gene050020:CDS	45.0%

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Chromosome	Type	Strat	End	Strand	Name
chr5.4	gene	51440438	51441241	51440438	ID=MS.gene050020
chr5.4	mRNA	51440438	51441241	51440438	ID=MS.gene050020.t1;Parent=MS.gene050020
chr5.4	exon	51440438	51441241	51440438	ID=MS.gene050020.t1.exon1;Parent=MS.gene050020.t1
chr5.4	CDS	51440438	51441241	51440438	ID=cds.MS.gene050020.t1;Parent=MS.gene050020.t1

Gene Sequence

>MS.gene050020

ATGGAATTTGATGAACAAGAGGAACAAGAAGAGGAGATGGAGATTCCAGAAACACAAACACCAGTGCCAACGAGTTATGACTCACTTGGTAACTCAGCTTCACGTTCAAAACTCACCGTTGGAGTTGACGGAAGGAAAGGTAACGGCAACGGCTGTTTTGCTTCGACGGTGAGGTACCGAGAATGTCAGAAGAATCACGCCGTTAGCTTCGGTGGCCATGCTGTTGACGGTTGTTGCGAGTTTATCGCTGCCGGTGAAGAAGGAACACTTGAAGCTGTTATTTGCGCCGCTTGTAACTGTCACCGGAACTTCCACCGCAAGGAGATCGATGGTGAAACGGTTAGTTCATGTAACCGGTCACAGCCACCACCTCCACCGCCGCAATACCATCACCACAGCAATCAATTCTCGCCTTACTACCACCGTGCTCCTCCTTCCACAGCCGGTTACCTTCACCACCATCATCTTGCAACTCCGGTGGCACATCACAGACCGTTGGCTCTCCCTGCTGCTGCTTCCGGTGGTGGAATGAGCAGAGAAGATGATGATATCTCAAACCCTAGCAGTAGCGGTGGAGGTGGTGGTGGTGGTAGCGGTGGTGGTGGATCAAGGAAGAGATTCAGAACAAAATTCACTCAGGAACAGAAAGAGAAGTTGCTGGCTTTTGCTGAAGAACATGGTTGGAGAATTCAGAAACAAGATGAAGCTGCTATAGAACAGTTTTGTGCTGAGAATTGTATCAAGAGAAATGTTCTTAAAGTTTGGATGCACAATAACAAGAACACTCTTGGTAAGAAACCCTAA

Protein sequence

>MS.gene050020.t1

MEFDEQEEQEEEMEIPETQTPVPTSYDSLGNSASRSKLTVGVDGRKGNGNGCFASTVRYRECQKNHAVSFGGHAVDGCCEFIAAGEEGTLEAVICAACNCHRNFHRKEIDGETVSSCNRSQPPPPPPQYHHHSNQFSPYYHRAPPSTAGYLHHHHLATPVAHHRPLALPAAASGGGMSREDDDISNPSSSGGGGGGGSGGGGSRKRFRTKFTQEQKEKLLAFAEEHGWRIQKQDEAAIEQFCAENCIKRNVLKVWMHNNKNTLGKKP