AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene068233

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene068233.t1 MTR_1g105910 85.802 162 23 0 1 162 1 162 9.67e-97 279
MS.gene068233.t1 MTR_8g043650 84.049 163 26 0 1 163 1 163 8.99e-95 274
MS.gene068233.t1 MTR_1g105920 82.209 163 29 0 1 163 1 163 2.89e-93 270
MS.gene068233.t1 MTR_1g012570 81.595 163 30 0 1 163 1 163 1.50e-91 266
MS.gene068233.t1 MTR_3g052920 79.012 162 34 0 1 162 1 162 8.75e-91 264
MS.gene068233.t1 MTR_1g106070 77.914 163 36 0 1 163 1 163 5.89e-89 258
MS.gene068233.t1 MTR_2g030740 79.141 163 34 0 1 163 1 163 2.68e-88 258
MS.gene068233.t1 MTR_1g108510 71.605 162 46 0 1 162 1 162 2.37e-78 232
MS.gene068233.t1 MTR_3g030770 71.605 162 46 0 1 162 1 162 9.11e-78 231
MS.gene068233.t1 MTR_1g105905 76.027 146 35 0 18 163 1 146 4.75e-75 224
MS.gene068233.t1 MTR_1g108580 70.370 162 48 0 1 162 1 162 6.72e-75 224
MS.gene068233.t1 MTR_2g093190 69.753 162 49 0 1 162 1 162 1.74e-74 223
MS.gene068233.t1 MTR_5g045560 65.644 163 56 0 1 163 1 163 5.36e-70 211
MS.gene068233.t1 MTR_3g052870 84.127 126 20 0 1 126 1 126 1.46e-69 210
MS.gene068233.t1 MTR_0121s0080 67.901 162 52 0 1 162 1 162 1.53e-68 208
MS.gene068233.t1 MTR_1g075600 62.963 162 60 0 1 162 1 162 5.42e-66 201
MS.gene068233.t1 MTR_5g053390 61.905 168 54 2 1 163 1 163 7.44e-66 201
MS.gene068233.t1 MTR_1g077360 61.350 163 63 0 1 163 1 163 1.02e-65 201
MS.gene068233.t1 MTR_2g085250 61.111 162 63 0 1 162 1 162 5.47e-65 199
MS.gene068233.t1 MTR_2g085280 61.111 162 63 0 1 162 1 162 5.72e-65 199
MS.gene068233.t1 MTR_5g055100 60.736 163 64 0 1 163 1 163 7.58e-64 196
MS.gene068233.t1 MTR_0121s0100 65.152 132 46 0 1 132 1 132 2.05e-53 166
MS.gene068233.t1 MTR_3g080940 51.948 154 74 0 7 160 7 160 1.96e-52 166
MS.gene068233.t1 MTR_7g062350 60.135 148 58 1 16 162 27 174 1.63e-51 165
MS.gene068233.t1 MTR_3g030780 63.636 132 48 0 1 132 1 132 7.53e-50 157
MS.gene068233.t1 MTR_1g075570 53.846 130 46 1 18 147 1 116 5.86e-40 133
MS.gene068233.t1 MTR_8g086290 41.667 156 87 2 5 160 8 159 4.46e-35 120
MS.gene068233.t1 MTR_2g105290 39.744 156 90 1 5 160 8 159 4.61e-34 118
MS.gene068233.t1 MTR_3g067910 55.340 103 46 0 21 123 2 104 1.28e-31 110
MS.gene068233.t1 MTR_7g028448 41.290 155 79 2 5 159 8 150 6.67e-31 110
MS.gene068233.t1 MTR_4g036915 41.290 155 79 2 5 159 8 150 6.67e-31 110
MS.gene068233.t1 MTR_3g067875 75.000 68 17 0 18 85 1 68 1.33e-29 103
MS.gene068233.t1 MTR_1g108500 59.794 97 18 1 22 118 110 185 5.52e-27 103
MS.gene068233.t1 MTR_4g127140 38.095 168 86 4 5 160 8 169 3.85e-25 95.9
MS.gene068233.t1 MTR_1g047550 59.420 69 28 0 9 77 2 70 1.17e-23 91.7
MS.gene068233.t1 MTR_1g063160 45.133 113 55 4 9 118 2 110 2.08e-23 91.3
MS.gene068233.t1 MTR_8g079502 57.746 71 30 0 9 79 2 72 3.32e-23 90.5
MS.gene068233.t1 MTR_3g067870 69.841 63 19 0 16 78 83 145 3.92e-23 89.7
MS.gene068233.t1 MTR_8g046350 38.462 130 72 2 9 135 2 126 2.79e-22 87.8
MS.gene068233.t1 MTR_1g054265 41.803 122 65 3 9 128 2 119 5.93e-22 87.0
MS.gene068233.t1 MTR_8g051580 44.118 102 53 2 9 109 2 100 1.66e-21 86.3
MS.gene068233.t1 MTR_4g131030 52.113 71 34 0 9 79 2 72 4.57e-21 85.9
MS.gene068233.t1 MTR_4g094638 42.202 109 56 2 9 113 2 107 6.02e-19 79.3
MS.gene068233.t1 MTR_5g032520 37.121 132 71 3 10 131 3 132 1.72e-18 79.3
MS.gene068233.t1 MTR_5g032150 36.364 132 72 3 10 131 3 132 2.13e-18 79.3
MS.gene068233.t1 MTR_5g066180 36.875 160 84 4 9 161 2 151 4.34e-18 78.2
MS.gene068233.t1 MTR_4g094632 39.623 106 62 1 9 114 2 105 4.85e-18 76.6
MS.gene068233.t1 MTR_4g093970 57.576 66 28 0 10 75 29 94 1.11e-17 77.4
MS.gene068233.t1 MTR_4g102530 52.113 71 34 0 10 80 3 73 1.15e-17 76.6
MS.gene068233.t1 MTR_3g452380 51.351 74 36 0 3 76 11 84 6.29e-17 75.5
MS.gene068233.t1 MTR_3g005530 50.000 68 34 0 9 76 2 69 7.27e-17 75.1
MS.gene068233.t1 MTR_1g101970 50.000 68 34 0 9 76 2 69 2.29e-16 73.2
MS.gene068233.t1 MTR_8g033270 51.471 68 33 0 9 76 2 69 2.32e-16 74.3
MS.gene068233.t1 MTR_6g464720 38.843 121 70 3 9 126 2 121 2.62e-16 73.9
MS.gene068233.t1 MTR_8g033220 50.000 74 35 1 10 83 3 74 3.48e-16 73.2
MS.gene068233.t1 MTR_8g033220 50.000 74 35 1 10 83 3 74 4.10e-16 73.2
MS.gene068233.t1 MTR_7g075870 47.436 78 39 1 10 87 3 78 5.30e-16 72.4
MS.gene068233.t1 MTR_4g109830 47.826 69 35 1 9 76 2 70 1.08e-15 72.0
MS.gene068233.t1 MTR_8g087860 49.333 75 38 0 2 76 11 85 1.11e-15 72.4
MS.gene068233.t1 MTR_1g053070 49.296 71 36 0 9 79 2 72 1.16e-15 72.4
MS.gene068233.t1 MTR_8g022970 31.544 149 99 2 8 155 17 163 1.51e-15 70.9
MS.gene068233.t1 MTR_2g009890 48.529 68 35 0 9 76 2 69 3.09e-15 71.6
MS.gene068233.t1 MTR_2g017865 50.000 68 34 0 9 76 17 84 5.46e-15 70.5
MS.gene068233.t1 MTR_0003s0590 50.000 68 34 0 9 76 2 69 6.73e-15 70.1
MS.gene068233.t1 MTR_7g075870 46.835 79 39 2 10 87 3 79 7.40e-15 69.3
MS.gene068233.t1 MTR_7g016630 42.105 76 43 1 9 83 2 77 1.00e-14 69.7
MS.gene068233.t1 MTR_3g102570 47.887 71 36 1 9 78 2 72 1.01e-14 70.5
MS.gene068233.t1 MTR_3g437790 34.965 143 76 5 26 159 30 164 1.09e-14 68.6
MS.gene068233.t1 MTR_8g066260 46.377 69 36 1 9 76 2 70 1.43e-14 69.3
MS.gene068233.t1 MTR_5g046790 45.070 71 36 1 9 79 2 69 1.96e-14 68.2
MS.gene068233.t1 MTR_2g461710 53.571 56 26 0 9 64 2 57 2.62e-14 64.3
MS.gene068233.t1 MTR_4g084740 46.479 71 37 1 9 78 2 72 2.65e-14 69.3
MS.gene068233.t1 MTR_5g046870 47.826 69 35 1 9 76 2 70 3.39e-14 65.5
MS.gene068233.t1 MTR_5g031000 48.529 68 35 0 9 76 2 69 3.56e-14 67.8
MS.gene068233.t1 MTR_5g031000 48.529 68 35 0 9 76 2 69 3.63e-14 68.2
MS.gene068233.t1 MTR_5g021270 47.143 70 35 1 9 76 2 71 4.25e-14 67.8
MS.gene068233.t1 MTR_5g021270 47.143 70 35 1 9 76 2 71 4.28e-14 67.8
MS.gene068233.t1 MTR_6g015975 46.377 69 36 1 9 76 2 70 1.10e-13 67.0
MS.gene068233.t1 MTR_1g038300 47.143 70 35 1 9 76 2 71 2.45e-13 65.9
MS.gene068233.t1 MTR_7g016600 46.377 69 36 1 9 76 2 70 2.45e-13 65.9
MS.gene068233.t1 MTR_1g029670 42.857 70 38 1 9 76 2 71 2.96e-13 64.7
MS.gene068233.t1 MTR_5g066960 47.368 57 30 0 9 65 2 58 6.33e-13 60.8
MS.gene068233.t1 MTR_3g084980 36.937 111 61 2 9 110 2 112 8.18e-13 64.3
MS.gene068233.t1 MTR_7g075850 45.714 70 36 1 9 76 2 71 9.35e-13 64.3
MS.gene068233.t1 MTR_8g097090 46.377 69 36 1 9 76 2 70 9.71e-13 64.3
MS.gene068233.t1 MTR_3g088615 40.506 79 44 2 9 85 2 79 1.20e-12 63.2
MS.gene068233.t1 MTR_4g093030 32.468 154 75 5 9 135 2 153 2.07e-12 62.0
MS.gene068233.t1 MTR_4g109810 50.000 56 28 0 9 64 2 57 2.84e-12 62.8
MS.gene068233.t1 MTR_3g109930 30.000 140 82 3 27 159 20 150 4.05e-12 61.6
MS.gene068233.t1 MTR_3g113030 46.032 63 34 0 9 71 2 64 4.31e-12 62.4
MS.gene068233.t1 MTR_4g051538 43.038 79 45 0 1 79 1 79 6.43e-12 62.0
MS.gene068233.t1 MTR_2g045020 30.435 161 88 6 7 159 21 165 3.98e-11 58.9
MS.gene068233.t1 MTR_4g036050 40.580 69 40 1 10 77 3 71 6.25e-11 58.2
MS.gene068233.t1 MTR_4g036050 40.580 69 40 1 10 77 3 71 7.04e-11 58.5
MS.gene068233.t1 MTR_4g036050 40.000 70 41 1 9 77 2 71 7.41e-11 58.2
MS.gene068233.t1 MTR_4g036050 40.000 70 41 1 9 77 2 71 7.75e-11 58.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene068233.t1 AT5G60440 54.167 168 68 4 5 166 3 167 6.40e-52 167
MS.gene068233.t1 AT2G24840 47.239 163 81 3 6 165 60 220 1.07e-42 143
MS.gene068233.t1 AT4G36590 46.914 162 80 3 5 160 3 164 3.71e-38 130
MS.gene068233.t1 AT1G01530 43.293 164 79 4 4 157 2 161 2.43e-34 121
MS.gene068233.t1 AT1G65360 40.373 161 89 3 5 162 3 159 2.35e-30 110
MS.gene068233.t1 AT2G34440 44.248 113 62 1 9 121 2 113 2.57e-25 95.9
MS.gene068233.t1 AT3G66656 41.221 131 72 3 9 134 2 132 8.30e-24 92.0
MS.gene068233.t1 AT3G04100 28.655 171 112 2 2 165 7 174 1.61e-20 84.3
MS.gene068233.t1 AT1G72350 52.778 72 32 1 9 80 43 112 8.28e-19 80.1
MS.gene068233.t1 AT4G22950 40.909 110 62 1 10 119 3 109 8.73e-19 79.0
MS.gene068233.t1 AT4G22950 40.909 110 62 1 10 119 3 109 2.64e-18 79.0
MS.gene068233.t1 AT4G22950 40.909 110 62 1 10 119 3 109 2.64e-18 79.0
MS.gene068233.t1 AT2G45650 52.941 68 32 0 9 76 2 69 6.04e-18 78.6
MS.gene068233.t1 AT2G45660 49.351 77 37 1 10 86 3 77 6.29e-18 76.3
MS.gene068233.t1 AT4G09960 51.471 68 33 0 9 76 3 70 1.08e-17 77.4
MS.gene068233.t1 AT4G09960 51.471 68 33 0 9 76 2 69 1.09e-17 77.0
MS.gene068233.t1 AT4G09960 51.471 68 33 0 9 76 2 69 1.14e-17 77.4
MS.gene068233.t1 AT4G09960 51.471 68 33 0 9 76 12 79 1.58e-17 77.0
MS.gene068233.t1 AT2G45660 49.351 77 37 1 10 86 3 77 2.09e-17 76.3
MS.gene068233.t1 AT4G09960 51.471 68 33 0 9 76 28 95 2.24e-17 77.0
MS.gene068233.t1 AT4G09960 51.471 68 33 0 9 76 97 164 5.09e-17 77.0
MS.gene068233.t1 AT4G24540 37.879 132 74 3 9 132 2 133 5.41e-17 75.5
MS.gene068233.t1 AT3G61120 51.471 68 33 0 9 76 2 69 2.63e-16 73.9
MS.gene068233.t1 AT5G62165 46.250 80 41 1 10 89 3 80 2.94e-16 70.9
MS.gene068233.t1 AT3G57230 47.059 68 36 0 9 76 2 69 3.63e-16 71.6
MS.gene068233.t1 AT5G62165 46.250 80 41 1 10 89 3 80 3.71e-16 71.6
MS.gene068233.t1 AT5G62165 46.250 80 41 1 10 89 3 80 3.71e-16 71.6
MS.gene068233.t1 AT5G62165 46.250 80 41 1 10 89 3 80 3.71e-16 71.6
MS.gene068233.t1 AT2G22540 52.239 67 32 0 10 76 3 69 4.49e-16 73.2
MS.gene068233.t1 AT2G22540 52.239 67 32 0 10 76 3 69 4.49e-16 73.2
MS.gene068233.t1 AT4G18960 44.231 104 47 3 5 108 15 107 5.12e-16 73.2
MS.gene068233.t1 AT5G62165 46.250 80 41 1 10 89 3 80 5.54e-16 71.2
MS.gene068233.t1 AT5G62165 46.250 80 41 1 10 89 3 80 5.54e-16 71.2
MS.gene068233.t1 AT5G62165 46.250 80 41 1 10 89 3 80 5.54e-16 71.2
MS.gene068233.t1 AT5G62165 46.250 80 41 1 10 89 3 80 5.54e-16 71.2
MS.gene068233.t1 AT5G62165 46.250 80 41 1 10 89 3 80 5.54e-16 71.2
MS.gene068233.t1 AT2G42830 51.471 68 33 0 9 76 17 84 8.95e-16 72.4
MS.gene068233.t1 AT2G42830 51.471 68 33 0 9 76 17 84 9.29e-16 72.4
MS.gene068233.t1 AT4G11880 48.611 72 36 1 10 80 3 74 1.29e-15 70.9
MS.gene068233.t1 AT4G11880 48.611 72 36 1 10 80 3 74 1.29e-15 70.9
MS.gene068233.t1 AT3G58780 50.704 71 35 0 6 76 14 84 1.40e-15 72.0
MS.gene068233.t1 AT3G58780 50.704 71 35 0 6 76 20 90 1.50e-15 72.0
MS.gene068233.t1 AT1G28460 30.000 140 95 1 5 141 4 143 1.56e-15 70.9
MS.gene068233.t1 AT3G57230 47.059 68 36 0 9 76 2 69 1.60e-15 71.6
MS.gene068233.t1 AT3G57230 47.059 68 36 0 9 76 2 69 1.60e-15 71.6
MS.gene068233.t1 AT5G51870 50.746 67 33 0 10 76 3 69 1.60e-15 70.5
MS.gene068233.t1 AT3G58780 50.704 71 35 0 6 76 14 84 1.78e-15 72.4
MS.gene068233.t1 AT5G62165 46.250 80 41 1 10 89 3 80 1.80e-15 70.9
MS.gene068233.t1 AT5G62165 46.250 80 41 1 10 89 3 80 1.80e-15 70.9
MS.gene068233.t1 AT5G62165 46.250 80 41 1 10 89 3 80 1.91e-15 70.9
MS.gene068233.t1 AT5G62165 46.250 80 41 1 10 89 3 80 1.91e-15 70.9
MS.gene068233.t1 AT5G62165 46.250 80 41 1 10 89 3 80 1.91e-15 70.9
MS.gene068233.t1 AT5G51870 50.746 67 33 0 10 76 3 69 2.27e-15 70.9
MS.gene068233.t1 AT3G30260 30.201 149 86 4 9 156 2 133 2.35e-15 71.6
MS.gene068233.t1 AT5G51870 50.746 67 33 0 10 76 26 92 2.40e-15 70.5
MS.gene068233.t1 AT4G11880 47.945 73 37 1 10 81 3 75 2.95e-15 70.9
MS.gene068233.t1 AT4G11880 47.945 73 37 1 10 81 3 75 2.95e-15 70.9
MS.gene068233.t1 AT1G77950 46.479 71 37 1 9 78 2 72 3.28e-15 71.2
MS.gene068233.t1 AT1G77950 46.479 71 37 1 9 78 2 72 3.28e-15 71.2
MS.gene068233.t1 AT1G77950 46.479 71 37 1 9 78 2 72 3.28e-15 71.2
MS.gene068233.t1 AT1G77950 46.479 71 37 1 9 78 2 72 3.28e-15 71.2
MS.gene068233.t1 AT5G51870 50.746 67 33 0 10 76 3 69 3.63e-15 70.5
MS.gene068233.t1 AT5G51860 37.864 103 64 0 10 112 3 105 3.70e-15 70.1
MS.gene068233.t1 AT5G51860 37.864 103 64 0 10 112 3 105 4.20e-15 70.1
MS.gene068233.t1 AT5G51870 50.746 67 33 0 10 76 13 79 4.53e-15 70.5
MS.gene068233.t1 AT3G57390 48.529 68 35 0 9 76 2 69 4.68e-15 70.5
MS.gene068233.t1 AT1G77980 47.887 71 36 1 9 78 2 72 5.46e-15 71.2
MS.gene068233.t1 AT5G60910 49.296 71 33 1 9 79 2 69 5.52e-15 70.5
MS.gene068233.t1 AT1G29962 27.381 168 113 3 5 166 4 168 5.82e-15 69.3
MS.gene068233.t1 AT5G13790 50.000 68 34 0 9 76 2 69 6.31e-15 70.5
MS.gene068233.t1 AT5G13790 50.000 68 34 0 9 76 2 69 6.38e-15 70.5
MS.gene068233.t1 AT3G57390 48.529 68 35 0 9 76 2 69 6.83e-15 70.5
MS.gene068233.t1 AT1G69120 47.826 69 35 1 9 76 2 70 9.99e-15 69.7
MS.gene068233.t1 AT2G22630 47.059 68 36 0 9 76 2 69 1.10e-14 69.3
MS.gene068233.t1 AT2G22630 47.059 68 36 0 9 76 2 69 1.10e-14 69.3
MS.gene068233.t1 AT1G26310 47.826 69 35 1 9 76 2 70 1.14e-14 69.7
MS.gene068233.t1 AT1G22130 46.479 71 37 1 9 78 2 72 1.47e-14 70.1
MS.gene068233.t1 AT1G69120 47.826 69 35 1 9 76 2 70 1.75e-14 69.3
MS.gene068233.t1 AT4G37940 45.588 68 37 0 9 76 2 69 2.02e-14 68.6
MS.gene068233.t1 AT1G17310 51.316 76 35 1 8 83 47 120 2.07e-14 68.6
MS.gene068233.t1 AT4G37940 45.588 68 37 0 9 76 2 69 2.23e-14 68.6
MS.gene068233.t1 AT5G10140 47.826 69 35 1 9 76 2 70 2.52e-14 67.0
MS.gene068233.t1 AT4G37940 45.588 68 37 0 9 76 2 69 2.53e-14 67.4
MS.gene068233.t1 AT1G17310 51.316 76 35 1 8 83 58 131 2.74e-14 68.2
MS.gene068233.t1 AT5G10140 47.826 69 35 1 9 76 2 70 3.58e-14 67.0
MS.gene068233.t1 AT5G10140 47.826 69 35 1 9 76 2 70 4.66e-14 66.6
MS.gene068233.t1 AT5G10140 47.826 69 35 1 9 76 2 70 5.62e-14 67.0
MS.gene068233.t1 AT3G54340 47.619 63 33 0 9 71 2 64 6.71e-14 67.4
MS.gene068233.t1 AT2G22540 50.746 67 28 1 10 76 3 64 6.83e-14 67.4
MS.gene068233.t1 AT2G14210 46.377 69 36 1 9 76 2 70 7.18e-14 67.4
MS.gene068233.t1 AT2G14210 46.377 69 36 1 9 76 2 70 7.39e-14 67.0
MS.gene068233.t1 AT3G02310 47.826 69 35 1 9 76 2 70 1.14e-13 66.2
MS.gene068233.t1 AT5G20240 44.286 70 37 1 9 76 2 71 2.10e-13 64.3
MS.gene068233.t1 AT5G65050 31.452 124 69 3 9 118 2 123 2.10e-13 65.1
MS.gene068233.t1 AT5G23260 44.554 101 43 4 5 101 12 103 2.17e-13 66.2
MS.gene068233.t1 AT5G23260 44.554 101 43 4 5 101 12 103 2.27e-13 66.2
MS.gene068233.t1 AT5G65050 31.452 124 69 3 9 118 2 123 2.42e-13 65.1
MS.gene068233.t1 AT5G65050 31.452 124 69 3 9 118 2 123 2.93e-13 65.1
MS.gene068233.t1 AT5G23260 44.211 95 42 3 9 101 2 87 3.02e-13 65.5
MS.gene068233.t1 AT5G15800 46.377 69 36 1 9 76 2 70 3.35e-13 65.5
MS.gene068233.t1 AT2G03710 43.529 85 43 4 9 90 2 84 3.42e-13 64.7
MS.gene068233.t1 AT5G23260 44.211 95 42 3 9 101 2 87 3.45e-13 65.5
MS.gene068233.t1 AT5G15800 46.377 69 36 1 9 76 2 70 4.22e-13 65.5
MS.gene068233.t1 AT3G02310 47.826 69 35 1 9 76 2 70 5.18e-13 65.1
MS.gene068233.t1 AT5G65060 31.884 138 77 4 9 131 2 137 7.66e-13 63.9
MS.gene068233.t1 AT5G20240 44.286 70 37 1 9 76 2 71 8.87e-13 63.9
MS.gene068233.t1 AT5G65060 31.884 138 77 4 9 131 2 137 8.99e-13 63.5
MS.gene068233.t1 AT3G58780 51.667 60 29 0 6 65 14 73 1.08e-12 64.3
MS.gene068233.t1 AT1G47760 32.759 116 63 2 9 123 2 103 1.15e-12 63.2
MS.gene068233.t1 AT3G58780 51.667 60 29 0 6 65 14 73 1.22e-12 64.3
MS.gene068233.t1 AT1G24260 46.377 69 36 1 9 76 2 70 1.37e-12 63.9
MS.gene068233.t1 AT1G24260 46.377 69 36 1 9 76 2 70 1.42e-12 63.9
MS.gene068233.t1 AT1G24260 51.786 56 27 0 9 64 2 57 1.62e-12 63.5
MS.gene068233.t1 AT2G03710 42.529 87 45 4 9 92 2 86 1.70e-12 63.9
MS.gene068233.t1 AT2G03710 41.379 87 46 3 9 92 2 86 1.70e-12 63.9
MS.gene068233.t1 AT1G28450 28.966 145 93 3 5 141 4 146 2.63e-12 62.4
MS.gene068233.t1 AT5G65070 40.580 69 40 1 9 76 2 70 5.75e-12 60.5
MS.gene068233.t1 AT5G65050 43.478 69 38 1 9 76 2 70 6.88e-12 61.2
MS.gene068233.t1 AT1G46408 33.065 124 64 4 10 119 5 123 7.03e-12 62.0
MS.gene068233.t1 AT1G71692 42.029 69 39 1 10 77 3 71 9.56e-12 61.2
MS.gene068233.t1 AT5G65060 43.478 69 38 1 9 76 2 70 1.65e-11 60.1
MS.gene068233.t1 AT5G65070 40.580 69 40 1 9 76 2 70 2.03e-11 60.1
MS.gene068233.t1 AT5G65070 40.580 69 40 1 9 76 2 70 3.06e-11 60.1
MS.gene068233.t1 AT5G65070 40.580 69 40 1 9 76 2 70 3.66e-11 59.7
MS.gene068233.t1 AT4G37435 37.500 80 48 2 9 87 2 80 4.85e-11 58.9

Find 34 sgRNAs with CRISPR-Local

Find 47 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTTCTCCATGTCCAGTAAC+TGG 0.302941 8.1:-61055783 None:intergenic
TGGTGGGCTGGTCCCGTTGA+TGG 0.316276 8.1:+61055856 MS.gene068233:CDS
AGGCGACCGAGGCTCAATTT+TGG 0.319875 8.1:+61055836 MS.gene068233:CDS
GACTCAGATCAACCAGTTAC+TGG 0.372422 8.1:+61055771 MS.gene068233:CDS
TTTGGATGAGACTAAGAAAC+TGG 0.404063 8.1:+61055915 MS.gene068233:CDS
ATTGCATGGTACCATTGTTT+TGG 0.409018 8.1:-61055698 None:intergenic
GTCCCGTTGATGGGATGAAT+AGG 0.432754 8.1:+61055866 MS.gene068233:CDS
GGTGGGCTGGTCCCGTTGAT+GGG 0.452333 8.1:+61055857 MS.gene068233:CDS
TCCCGTTGATGGGATGAATA+GGG 0.453040 8.1:+61055867 MS.gene068233:CDS
CCTCGGTCGCCTTGCGCAAG+TGG 0.465805 8.1:-61055825 None:intergenic
CTCTATGACCGTATCAACAT+TGG 0.477809 8.1:-61055653 None:intergenic
CTCACGCACATTGGCGCTAA+CGG 0.479728 8.1:-61055734 None:intergenic
GCCCTATTCATCCCATCAAC+GGG 0.481029 8.1:-61055868 None:intergenic
ACTGAGAGTGCAAAGCTCAC+TGG 0.526727 8.1:-61055569 None:intergenic
GGCCCTATTCATCCCATCAA+CGG 0.536262 8.1:-61055869 None:intergenic
TCTATGACCGTATCAACATT+GGG 0.539713 8.1:-61055652 None:intergenic
TGGCCAACCCAATGTTGATA+CGG 0.545005 8.1:+61055645 MS.gene068233:CDS
CCACTTGCGCAAGGCGACCG+AGG 0.551221 8.1:+61055825 MS.gene068233:CDS
CTTTCTCGAAGCGCCGTAGT+GGG 0.553366 8.1:+61055533 MS.gene068233:CDS
ATTCAACCATGTCGAGTGGA+AGG 0.567884 8.1:+61055446 None:intergenic
ACTTTCTCGAAGCGCCGTAG+TGG 0.571963 8.1:+61055532 MS.gene068233:CDS
GATCAACCAGTTACTGGACA+TGG 0.586550 8.1:+61055777 MS.gene068233:CDS
AATGAAAATACCTTCTCCCC+AGG 0.587583 8.1:-61055622 None:intergenic
AGCCCACCAAAATTGAGCCT+CGG 0.589062 8.1:-61055842 None:intergenic
TGACCGTATCAACATTGGGT+TGG 0.600758 8.1:-61055648 None:intergenic
AGTGAGCTTTGCACTCTCAG+TGG 0.609659 8.1:+61055571 MS.gene068233:CDS
AGAGCTGAACCACTTGCGCA+AGG 0.610527 8.1:+61055816 MS.gene068233:CDS
CTTTCTTGAAGAGCCCACTA+CGG 0.610979 8.1:-61055546 None:intergenic
AGTATTGAGCTCACGCACAT+TGG 0.613452 8.1:-61055743 None:intergenic
TGTGATTCAACCATGTCGAG+TGG 0.620546 8.1:+61055442 None:intergenic
TGGTACCATGCAATTCATTG+AGG 0.638950 8.1:+61055707 MS.gene068233:CDS
GTGAGCCTCAATGAATTGCA+TGG 0.640763 8.1:-61055712 None:intergenic
TCGAGTCCACACCAAAACAA+TGG 0.680094 8.1:+61055687 MS.gene068233:CDS
TCGAGTGGAAGGAAAAGCCG+AGG 0.691796 8.1:+61055457 MS.gene068233:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! CTTTTTGAAAAATTCAAGTT+GGG - chr8.1:61055893-61055912 None:intergenic 20.0%
!!! TTTTTTTCATCTCAATCTTT+TGG - chr8.1:61055486-61055505 None:intergenic 20.0%
!!! CCAACTTGAATTTTTCAAAA+AGG + chr8.1:61055891-61055910 MS.gene068233:CDS 25.0%
!!! CCTTTTTGAAAAATTCAAGT+TGG - chr8.1:61055894-61055913 None:intergenic 25.0%
!!! TGAATTTTTCAAAAAGGCTT+TGG + chr8.1:61055897-61055916 MS.gene068233:CDS 25.0%
TCTATGACCGTATCAACATT+GGG - chr8.1:61055655-61055674 None:intergenic 35.0%
TTTGGATGAGACTAAGAAAC+TGG + chr8.1:61055915-61055934 MS.gene068233:CDS 35.0%
! ATTGCATGGTACCATTGTTT+TGG - chr8.1:61055701-61055720 None:intergenic 35.0%
AATGAAAATACCTTCTCCCC+AGG - chr8.1:61055625-61055644 None:intergenic 40.0%
CTCTATGACCGTATCAACAT+TGG - chr8.1:61055656-61055675 None:intergenic 40.0%
TGGTACCATGCAATTCATTG+AGG + chr8.1:61055707-61055726 MS.gene068233:CDS 40.0%
!!! ATGGTACCATTGTTTTGGTG+TGG - chr8.1:61055696-61055715 None:intergenic 40.0%
!!! GGGGAGAAGGTATTTTCATT+TGG + chr8.1:61055625-61055644 MS.gene068233:CDS 40.0%
CTTTCTTGAAGAGCCCACTA+CGG - chr8.1:61055549-61055568 None:intergenic 45.0%
GACTCAGATCAACCAGTTAC+TGG + chr8.1:61055771-61055790 MS.gene068233:CDS 45.0%
GATCAACCAGTTACTGGACA+TGG + chr8.1:61055777-61055796 MS.gene068233:CDS 45.0%
GTGAGCCTCAATGAATTGCA+TGG - chr8.1:61055715-61055734 None:intergenic 45.0%
TCGAGTCCACACCAAAACAA+TGG + chr8.1:61055687-61055706 MS.gene068233:CDS 45.0%
TCTTCTCCATGTCCAGTAAC+TGG - chr8.1:61055786-61055805 None:intergenic 45.0%
TGACCGTATCAACATTGGGT+TGG - chr8.1:61055651-61055670 None:intergenic 45.0%
TGGCCAACCCAATGTTGATA+CGG + chr8.1:61055645-61055664 MS.gene068233:CDS 45.0%
! AGTATTGAGCTCACGCACAT+TGG - chr8.1:61055746-61055765 None:intergenic 45.0%
! TCCCGTTGATGGGATGAATA+GGG + chr8.1:61055867-61055886 MS.gene068233:CDS 45.0%
! TCTCAATCTTTTGGCGACCT+CGG - chr8.1:61055477-61055496 None:intergenic 45.0%
!! CTCTCGTTGTTTTCTCACCT+GGG + chr8.1:61055605-61055624 MS.gene068233:CDS 45.0%
!! TCTCGTTGTTTTCTCACCTG+GGG + chr8.1:61055606-61055625 MS.gene068233:CDS 45.0%
AGCCCACCAAAATTGAGCCT+CGG - chr8.1:61055845-61055864 None:intergenic 50.0%
AGTGAGCTTTGCACTCTCAG+TGG + chr8.1:61055571-61055590 MS.gene068233:CDS 50.0%
GCCCTATTCATCCCATCAAC+GGG - chr8.1:61055871-61055890 None:intergenic 50.0%
GGCCCTATTCATCCCATCAA+CGG - chr8.1:61055872-61055891 None:intergenic 50.0%
! ACTGAGAGTGCAAAGCTCAC+TGG - chr8.1:61055572-61055591 None:intergenic 50.0%
! GTCCCGTTGATGGGATGAAT+AGG + chr8.1:61055866-61055885 MS.gene068233:CDS 50.0%
! TGTTTTCTCACCTGGGGAGA+AGG + chr8.1:61055612-61055631 MS.gene068233:CDS 50.0%
!! GACCGAGGCTCAATTTTGGT+GGG + chr8.1:61055840-61055859 MS.gene068233:CDS 50.0%
!! GCTCTCGTTGTTTTCTCACC+TGG + chr8.1:61055604-61055623 MS.gene068233:CDS 50.0%
AGAGCTGAACCACTTGCGCA+AGG + chr8.1:61055816-61055835 MS.gene068233:CDS 55.0%
CTCACGCACATTGGCGCTAA+CGG - chr8.1:61055737-61055756 None:intergenic 55.0%
TCGAGTGGAAGGAAAAGCCG+AGG + chr8.1:61055457-61055476 MS.gene068233:CDS 55.0%
! AGGCGACCGAGGCTCAATTT+TGG + chr8.1:61055836-61055855 MS.gene068233:CDS 55.0%
! CGACCGAGGCTCAATTTTGG+TGG + chr8.1:61055839-61055858 MS.gene068233:CDS 55.0%
!! ACTTTCTCGAAGCGCCGTAG+TGG + chr8.1:61055532-61055551 MS.gene068233:CDS 55.0%
!! CTTTCTCGAAGCGCCGTAGT+GGG + chr8.1:61055533-61055552 MS.gene068233:CDS 55.0%
!! GAGGCTCAATTTTGGTGGGC+TGG + chr8.1:61055844-61055863 MS.gene068233:CDS 55.0%
!! GGTGGGCTGGTCCCGTTGAT+GGG + chr8.1:61055857-61055876 MS.gene068233:CDS 65.0%
!! TGGTGGGCTGGTCCCGTTGA+TGG + chr8.1:61055856-61055875 MS.gene068233:CDS 65.0%
CCACTTGCGCAAGGCGACCG+AGG + chr8.1:61055825-61055844 MS.gene068233:CDS 70.0%
CCTCGGTCGCCTTGCGCAAG+TGG - chr8.1:61055828-61055847 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr8.1 gene 61055454 61055954 61055454 ID=MS.gene068233
chr8.1 mRNA 61055454 61055954 61055454 ID=MS.gene068233.t1;Parent=MS.gene068233
chr8.1 exon 61055454 61055954 61055454 ID=MS.gene068233.t1.exon1;Parent=MS.gene068233.t1
chr8.1 CDS 61055454 61055954 61055454 ID=cds.MS.gene068233.t1;Parent=MS.gene068233.t1
Gene Sequence

>MS.gene068233

ATGTCGAGTGGAAGGAAAAGCCGAGGTCGCCAAAAGATTGAGATGAAAAAAATGAGCAACGAGAGCAACTTGCAAGTGACTTTCTCGAAGCGCCGTAGTGGGCTCTTCAAGAAAGCCAGTGAGCTTTGCACTCTCAGTGGTGCAGATGTTGCTCTCGTTGTTTTCTCACCTGGGGAGAAGGTATTTTCATTTGGCCAACCCAATGTTGATACGGTCATAGAGCGTTATCTCTCTCGAGTCCACACCAAAACAATGGTACCATGCAATTCATTGAGGCTCACCGTTAGCGCCAATGTGCGTGAGCTCAATACTCAGCTGACTCAGATCAACCAGTTACTGGACATGGAGAAGAAACGTGCTGAAGAGCTGAACCACTTGCGCAAGGCGACCGAGGCTCAATTTTGGTGGGCTGGTCCCGTTGATGGGATGAATAGGGCCCAACTTGAATTTTTCAAAAAGGCTTTGGATGAGACTAAGAAACTGGTTTGCACATCATGCTGA
Protein sequence

>MS.gene068233.t1

MSSGRKSRGRQKIEMKKMSNESNLQVTFSKRRSGLFKKASELCTLSGADVALVVFSPGEKVFSFGQPNVDTVIERYLSRVHTKTMVPCNSLRLTVSANVRELNTQLTQINQLLDMEKKRAEELNHLRKATEAQFWWAGPVDGMNRAQLEFFKKALDETKKLVCTSC