AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene068439

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene068439.t1 MTR_5g084870 85.615 431 60 1 1 429 1 431 0.0 779
MS.gene068439.t1 MTR_5g084850 80.876 434 74 4 1 429 1 430 0.0 724
MS.gene068439.t1 MTR_8g079970 77.346 437 91 3 1 429 1 437 0.0 701
MS.gene068439.t1 MTR_8g079950 74.828 437 102 4 1 429 1 437 0.0 682
MS.gene068439.t1 MTR_5g084900 76.862 376 58 3 56 429 6 354 0.0 591
MS.gene068439.t1 MTR_8g079980 58.636 440 167 5 1 429 1 436 0.0 514
MS.gene068439.t1 MTR_3g086020 37.705 427 240 12 1 419 3 411 1.51e-87 273
MS.gene068439.t1 MTR_2g007880 33.040 454 261 10 1 426 15 453 2.67e-74 239
MS.gene068439.t1 MTR_8g467580 30.973 452 275 13 1 427 1 440 4.02e-56 192
MS.gene068439.t1 MTR_8g467590 30.396 454 275 15 1 427 1 440 7.56e-55 188
MS.gene068439.t1 MTR_1g094790 30.472 466 262 16 1 426 1 444 1.47e-50 177
MS.gene068439.t1 MTR_7g110540 65.854 123 24 1 1 123 1 105 1.85e-47 163
MS.gene068439.t1 MTR_7g110540 46.053 152 47 4 238 389 104 220 2.12e-36 134
MS.gene068439.t1 MTR_4g127650 23.451 452 301 13 2 416 9 452 1.57e-25 108
MS.gene068439.t1 MTR_7g085990 25.319 470 277 20 3 416 2 453 5.14e-25 107
MS.gene068439.t1 MTR_8g096530 24.091 440 284 15 11 418 7 428 9.69e-25 106
MS.gene068439.t1 MTR_3g026950 24.717 441 271 17 3 397 2 427 3.58e-24 104
MS.gene068439.t1 MTR_7g085930 24.364 472 285 17 3 417 6 462 1.16e-23 103
MS.gene068439.t1 MTR_5g076700 25.604 414 239 18 9 385 16 397 1.22e-23 103
MS.gene068439.t1 MTR_5g084920 82.759 58 9 1 1 58 1 57 1.55e-23 93.6
MS.gene068439.t1 MTR_4g007540 21.277 423 282 17 27 416 25 429 3.59e-23 101
MS.gene068439.t1 MTR_2g105330 21.711 456 290 17 1 416 1 429 8.20e-23 100
MS.gene068439.t1 MTR_2g064495 21.491 456 291 17 1 416 1 429 8.35e-23 100
MS.gene068439.t1 MTR_4g013050 24.651 430 267 15 10 416 11 406 3.22e-22 98.6
MS.gene068439.t1 MTR_3g049150 24.330 448 297 14 10 424 9 447 3.30e-22 99.0
MS.gene068439.t1 MTR_1g040215 24.257 404 253 16 2 375 8 388 5.76e-22 98.2
MS.gene068439.t1 MTR_8g075610 22.556 399 269 16 41 413 30 414 1.21e-21 96.7
MS.gene068439.t1 MTR_3g098980 21.818 440 297 16 2 414 11 430 6.09e-21 94.7
MS.gene068439.t1 MTR_5g026320 23.776 429 275 13 2 397 17 426 6.68e-21 95.1
MS.gene068439.t1 MTR_7g084910 25.604 414 237 17 26 404 38 415 2.86e-19 89.7
MS.gene068439.t1 MTR_4g094842 26.573 429 256 19 3 390 4 414 3.85e-19 89.7
MS.gene068439.t1 MTR_4g055170 25.000 456 277 18 11 420 20 456 4.10e-19 89.7
MS.gene068439.t1 MTR_4g094838 23.973 438 260 15 17 419 29 428 4.36e-18 86.3
MS.gene068439.t1 MTR_1g053315 23.973 438 271 21 1 396 16 433 4.86e-18 86.3
MS.gene068439.t1 MTR_7g114560 30.769 169 105 5 9 169 16 180 1.84e-17 84.7
MS.gene068439.t1 MTR_7g086000 22.642 477 303 16 1 424 2 465 2.77e-17 84.0
MS.gene068439.t1 MTR_1g040315 22.222 315 219 11 2 299 8 313 5.98e-17 82.8
MS.gene068439.t1 MTR_7g027175 22.249 418 275 14 1 390 13 408 7.81e-17 82.4
MS.gene068439.t1 MTR_3g112180 22.273 440 277 14 10 413 19 429 8.05e-17 82.8
MS.gene068439.t1 MTR_7g014200 22.902 441 265 13 16 397 20 444 1.33e-16 82.0
MS.gene068439.t1 MTR_5g090830 24.286 420 255 17 3 375 10 413 1.95e-15 78.6
MS.gene068439.t1 MTR_7g114490 22.943 401 249 17 9 375 16 390 2.50e-15 78.2
MS.gene068439.t1 MTR_4g094835 25.326 383 228 17 51 395 57 419 3.09e-15 77.8
MS.gene068439.t1 MTR_4g022670 26.340 429 245 21 3 387 4 405 3.67e-15 77.4
MS.gene068439.t1 MTR_7g014620 24.586 423 258 16 24 403 13 417 6.09e-15 76.6
MS.gene068439.t1 MTR_3g100150 26.220 164 113 4 10 169 18 177 5.51e-14 73.9
MS.gene068439.t1 MTR_2g036780 26.506 166 114 4 10 171 19 180 6.68e-14 73.6
MS.gene068439.t1 MTR_8g041770 23.618 398 249 15 17 385 31 402 3.05e-13 71.6
MS.gene068439.t1 MTR_1g011660 26.923 156 107 4 22 172 23 176 8.68e-13 70.1
MS.gene068439.t1 MTR_3g102010 23.851 457 307 18 3 426 7 455 1.36e-12 69.7
MS.gene068439.t1 MTR_1g035890 26.190 168 114 5 10 171 18 181 1.93e-12 68.9
MS.gene068439.t1 MTR_3g071320 22.523 444 283 19 11 415 13 434 3.78e-12 68.2
MS.gene068439.t1 MTR_1g040195 21.189 387 247 15 9 375 15 363 6.53e-11 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene068439.t1 AT3G26040 34.292 452 253 13 1 427 1 433 5.96e-81 256
MS.gene068439.t1 AT1G24430 33.333 438 270 9 1 427 3 429 1.90e-72 234
MS.gene068439.t1 AT4G15390 31.757 444 280 12 1 427 6 443 4.39e-66 218
MS.gene068439.t1 AT4G15390 31.757 444 280 12 1 427 6 443 4.39e-66 218
MS.gene068439.t1 AT1G24420 35.714 448 244 17 3 426 5 432 6.31e-66 217
MS.gene068439.t1 AT1G24420 35.714 448 244 17 3 426 5 432 6.31e-66 217
MS.gene068439.t1 AT3G30280 31.696 448 274 15 1 427 4 440 3.68e-64 213
MS.gene068439.t1 AT5G23970 32.373 451 255 16 1 427 1 425 1.43e-62 208
MS.gene068439.t1 AT5G47950 31.166 446 262 14 1 427 4 423 2.22e-58 197
MS.gene068439.t1 AT5G47980 33.113 453 260 18 1 426 3 439 8.92e-58 196
MS.gene068439.t1 AT4G15400 28.855 454 268 18 2 426 4 431 2.09e-39 147
MS.gene068439.t1 AT2G19070 25.223 448 283 17 10 416 11 447 2.26e-27 114
MS.gene068439.t1 AT5G48930 24.263 441 294 17 1 414 1 428 3.37e-27 113
MS.gene068439.t1 AT5G41040 25.445 393 240 14 11 373 25 394 1.58e-25 108
MS.gene068439.t1 AT5G63560 24.009 429 276 16 11 413 17 421 1.83e-25 108
MS.gene068439.t1 AT5G41040 25.445 393 240 14 11 373 41 410 1.84e-25 108
MS.gene068439.t1 AT3G48720 24.027 437 274 18 9 413 15 425 2.31e-25 107
MS.gene068439.t1 AT5G67150 27.500 400 243 18 56 421 58 444 2.20e-20 93.6
MS.gene068439.t1 AT5G17540 22.374 438 296 16 9 417 15 437 2.83e-20 93.2
MS.gene068439.t1 AT3G50270 27.317 410 241 19 3 373 6 397 6.60e-20 92.0
MS.gene068439.t1 AT1G31490 23.834 386 255 11 11 373 14 383 7.05e-20 92.0
MS.gene068439.t1 AT5G57840 23.544 412 259 13 1 375 1 393 5.16e-19 89.4
MS.gene068439.t1 AT1G65450 24.324 444 276 18 11 417 26 446 3.07e-18 87.0
MS.gene068439.t1 AT5G38130 25.000 372 230 15 38 373 52 410 1.03e-14 76.3
MS.gene068439.t1 AT4G31910 23.176 466 289 18 2 416 8 455 1.35e-14 75.9
MS.gene068439.t1 AT1G78990 24.207 347 217 14 59 375 67 397 1.50e-14 75.9
MS.gene068439.t1 AT5G67160 25.364 343 220 13 55 373 52 382 7.58e-14 73.6
MS.gene068439.t1 AT5G07860 22.958 453 300 17 2 419 7 445 1.92e-13 72.4
MS.gene068439.t1 AT1G32910 23.797 374 230 14 38 375 46 400 7.47e-13 70.5
MS.gene068439.t1 AT1G27620 21.346 431 286 15 11 413 17 422 2.43e-12 68.9
MS.gene068439.t1 AT5G42830 24.449 454 285 21 11 424 15 450 6.58e-12 67.4
MS.gene068439.t1 AT3G50300 26.330 376 228 17 38 376 32 395 2.16e-11 65.9

Find 74 sgRNAs with CRISPR-Local

Find 103 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTTCTTCTCTAAATAATTT+TGG 0.136369 6.3:+64499536 None:intergenic
TTCCATCTTCATCAAGATTT+TGG 0.150733 6.3:+64499990 None:intergenic
TACCACAATCTCTGGGAAAA+TGG 0.164083 6.3:+64499898 None:intergenic
CCAATGTATGAAGCGGATTT+TGG 0.170583 6.3:-64499422 MS.gene068439:CDS
AAAACAATGTGGTATTTAAA+AGG 0.222238 6.3:-64499868 MS.gene068439:CDS
CCAACATGGGTGACCACTTT+TGG 0.236794 6.3:-64499389 MS.gene068439:CDS
ATCATTCATGAAGTTGAAAA+TGG 0.240127 6.3:+64500027 None:intergenic
CAAGCACGCTGTTTCAATAA+TGG 0.246941 6.3:-64499750 MS.gene068439:CDS
CATCATGTCTAAACATTGTT+TGG 0.259672 6.3:+64499230 None:intergenic
TTTGGTCTCTACTCTTGTTT+GGG 0.296612 6.3:+64500371 None:intergenic
GTTTATTTGAATGGCTATAA+AGG 0.308022 6.3:+64500114 None:intergenic
GGAAATGGAAATAGAGGATT+TGG 0.317777 6.3:-64499603 MS.gene068439:CDS
AAGCACGCTGTTTCAATAAT+GGG 0.324962 6.3:-64499749 MS.gene068439:CDS
ATTTGCATGAGTCACAAAAT+TGG 0.329140 6.3:-64500061 MS.gene068439:CDS
TCTCTCTTTACCACAATCTC+TGG 0.338772 6.3:+64499890 None:intergenic
TCTTTCCATTGCAATTCATC+AGG 0.366379 6.3:+64500154 None:intergenic
TGCAAAACAGTTTATTTGAA+TGG 0.367290 6.3:+64500105 None:intergenic
CGTTGGTGATGAAGGTTTGA+TGG 0.376629 6.3:+64500477 None:intergenic
TCACCCTTTAGTTTACTTGA+TGG 0.383738 6.3:-64499953 MS.gene068439:CDS
GAACATGGAAGATAATTATA+TGG 0.400217 6.3:-64499300 MS.gene068439:CDS
GTTGGCAAATAAGAAGGAAA+TGG 0.414374 6.3:-64499618 MS.gene068439:CDS
TTTCAACTTCATGAATGATT+GGG 0.414945 6.3:-64500023 MS.gene068439:CDS
TTCAATGTTTAGCATGGTTG+TGG 0.421467 6.3:-64499711 MS.gene068439:CDS
ACCACAATCTCTGGGAAAAT+GGG 0.431736 6.3:+64499899 None:intergenic
CCCTTGCTCATTGCATTCAA+TGG 0.431972 6.3:+64500261 None:intergenic
TAAGAAGGAAATGGAAATAG+AGG 0.434120 6.3:-64499609 MS.gene068439:CDS
TGAGAAGGCGTTGGTGATGA+AGG 0.434663 6.3:+64500469 None:intergenic
TGCAGTGATTAAGTCTCTTA+AGG 0.436582 6.3:-64499831 MS.gene068439:CDS
AACCAAAATCTTGATGAAGA+TGG 0.437587 6.3:-64499992 MS.gene068439:CDS
TGCAATGGAAAGACATGGAT+TGG 0.442817 6.3:-64500144 MS.gene068439:CDS
GTCTATGTTGGCAAATAAGA+AGG 0.447511 6.3:-64499624 MS.gene068439:CDS
CCAAAATCCGCTTCATACAT+TGG 0.450217 6.3:+64499422 None:intergenic
TCCTCCATCAAGTAAACTAA+AGG 0.453406 6.3:+64499949 None:intergenic
AAAGTGTAGGGAACATACTT+TGG 0.455506 6.3:-64499652 MS.gene068439:CDS
TTGGTCTGTCAAATAAAAGA+AGG 0.457762 6.3:-64499584 MS.gene068439:CDS
AAGTAATGACACATAGTTAC+GGG 0.468479 6.3:+64500405 None:intergenic
TGTGGATCTTCGCAGAAGAA+TGG 0.486691 6.3:-64499693 MS.gene068439:CDS
CCATGCTAAACATTGAAGTC+TGG 0.488106 6.3:+64499717 None:intergenic
AAGAGAGTGGATAAAGTTTG+AGG 0.488221 6.3:+64500446 None:intergenic
GCAATTCATCAGGGAACAAA+GGG 0.500489 6.3:+64500164 None:intergenic
CTCTCTTTACCACAATCTCT+GGG 0.506292 6.3:+64499891 None:intergenic
CCTCCATCAAGTAAACTAAA+GGG 0.507177 6.3:+64499950 None:intergenic
TGGATACAAGAGATGGAGAT+GGG 0.510515 6.3:-64499337 MS.gene068439:CDS
TTAAGAGACTTAATCACTGC+AGG 0.520163 6.3:+64499833 None:intergenic
AGAAAGTAATTCTATTTCCA+TGG 0.521761 6.3:+64500507 None:intergenic
CCATTGAATGCAATGAGCAA+GGG 0.525582 6.3:-64500261 MS.gene068439:CDS
CCTTACAGAACAACCAAAAG+TGG 0.529496 6.3:+64499376 None:intergenic
AAAATGGGCAAATCCCTTTG+TGG 0.529925 6.3:+64499914 None:intergenic
CCAGACTTCAATGTTTAGCA+TGG 0.535069 6.3:-64499717 MS.gene068439:CDS
TGCAATTCATCAGGGAACAA+AGG 0.535516 6.3:+64500163 None:intergenic
CTTTCCATTGCAATTCATCA+GGG 0.536177 6.3:+64500155 None:intergenic
ATGGATACAAGAGATGGAGA+TGG 0.539300 6.3:-64499338 MS.gene068439:CDS
GGATAAAGTTTGAGGTGAGA+AGG 0.540442 6.3:+64500454 None:intergenic
AAAGTAATGACACATAGTTA+CGG 0.546947 6.3:+64500404 None:intergenic
AACATTGTTTGGATTAAGAG+AGG 0.547055 6.3:+64499241 None:intergenic
ACCATTGAATGCAATGAGCA+AGG 0.558967 6.3:-64500262 MS.gene068439:CDS
GATTGAAGCGATTGTGAACA+TGG 0.562184 6.3:-64499315 MS.gene068439:CDS
GATTAAGTCTCTTAAGGCAA+TGG 0.568628 6.3:-64499825 MS.gene068439:CDS
CCCTTTAGTTTACTTGATGG+AGG 0.573653 6.3:-64499950 MS.gene068439:CDS
CCAAAAGTGGTCACCCATGT+TGG 0.577438 6.3:+64499389 None:intergenic
AGTTACAACTTGTACACGTG+TGG 0.588043 6.3:+64499784 None:intergenic
TGGTCTGTCAAATAAAAGAA+GGG 0.590588 6.3:-64499583 MS.gene068439:CDS
TTGGATGGGGAAAACCAACA+TGG 0.595660 6.3:-64499403 MS.gene068439:CDS
TGTTCCCTGATGAATTGCAA+TGG 0.602006 6.3:-64500159 MS.gene068439:CDS
TCGGTTTCCAATGTATGAAG+CGG 0.603496 6.3:-64499429 MS.gene068439:CDS
AGTTTGAGGTGAGAAGGCGT+TGG 0.614243 6.3:+64500460 None:intergenic
AGCACGCTGTTTCAATAATG+GGG 0.615102 6.3:-64499748 MS.gene068439:CDS
CATTGAATGCAATGAGCAAG+GGG 0.621462 6.3:-64500260 MS.gene068439:CDS
ATGTTGTCTATGAAAGAGAG+TGG 0.633995 6.3:+64500433 None:intergenic
TGAATTGCAATGGAAAGACA+TGG 0.639854 6.3:-64500149 MS.gene068439:CDS
GTTACAACTTGTACACGTGT+GGG 0.659137 6.3:+64499785 None:intergenic
TGGATGGGGAAAACCAACAT+GGG 0.660202 6.3:-64499402 MS.gene068439:CDS
GTGTACAAGTTGTAACTGCG+TGG 0.696651 6.3:-64499778 MS.gene068439:CDS
GGTAAAGAGAGAAAACAATG+TGG 0.722617 6.3:-64499879 MS.gene068439:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAACAATGTGGTATTTAAA+AGG - chr6.3:64499875-64499894 MS.gene068439:CDS 20.0%
!!! AAACTTTTATTCTCAGAATA+TGG + chr6.3:64500273-64500292 None:intergenic 20.0%
!!! AAGGAATATTGTAATTTTGA+TGG - chr6.3:64500386-64500405 MS.gene068439:CDS 20.0%
!!! TCTTCTTCTCTAAATAATTT+TGG + chr6.3:64500210-64500229 None:intergenic 20.0%
! AAAGTAATGACACATAGTTA+CGG + chr6.3:64499342-64499361 None:intergenic 25.0%
! ATCATTCATGAAGTTGAAAA+TGG + chr6.3:64499719-64499738 None:intergenic 25.0%
! GAACATGGAAGATAATTATA+TGG - chr6.3:64500443-64500462 MS.gene068439:CDS 25.0%
! GTTTATTTGAATGGCTATAA+AGG + chr6.3:64499632-64499651 None:intergenic 25.0%
! TGCAAAACAGTTTATTTGAA+TGG + chr6.3:64499641-64499660 None:intergenic 25.0%
! TTTCAACTTCATGAATGATT+GGG - chr6.3:64499720-64499739 MS.gene068439:CDS 25.0%
!! CATTACATGAAAAAAGTGTA+GGG - chr6.3:64500079-64500098 MS.gene068439:CDS 25.0%
!! TCTAAATACTATCATTTTGC+TGG - chr6.3:64499439-64499458 MS.gene068439:CDS 25.0%
!! TTTTCAACTTCATGAATGAT+TGG - chr6.3:64499719-64499738 MS.gene068439:CDS 25.0%
!!! GTTTGTTTACTTTTAGTAGT+TGG - chr6.3:64500289-64500308 MS.gene068439:CDS 25.0%
AACATTGTTTGGATTAAGAG+AGG + chr6.3:64500505-64500524 None:intergenic 30.0%
AACCAAAATCTTGATGAAGA+TGG - chr6.3:64499751-64499770 MS.gene068439:CDS 30.0%
AAGTAATGACACATAGTTAC+GGG + chr6.3:64499341-64499360 None:intergenic 30.0%
ATTTGCATGAGTCACAAAAT+TGG - chr6.3:64499682-64499701 MS.gene068439:CDS 30.0%
TAAGAAGGAAATGGAAATAG+AGG - chr6.3:64500134-64500153 MS.gene068439:CDS 30.0%
TGGTCTGTCAAATAAAAGAA+GGG - chr6.3:64500160-64500179 MS.gene068439:CDS 30.0%
TTGGTCTGTCAAATAAAAGA+AGG - chr6.3:64500159-64500178 MS.gene068439:CDS 30.0%
! AATACTATCATTTTGCTGGT+AGG - chr6.3:64499443-64499462 MS.gene068439:CDS 30.0%
! CAAATAAACTGTTTTGCATG+TGG - chr6.3:64499643-64499662 MS.gene068439:CDS 30.0%
! CCATTACATGAAAAAAGTGT+AGG - chr6.3:64500078-64500097 MS.gene068439:CDS 30.0%
! TTCCATCTTCATCAAGATTT+TGG + chr6.3:64499756-64499775 None:intergenic 30.0%
!! ATTTTGATGGATACAAGAGA+TGG - chr6.3:64500399-64500418 MS.gene068439:CDS 30.0%
!! TTACTTTTAGTAGTTGGTGT+CGG - chr6.3:64500295-64500314 MS.gene068439:CDS 30.0%
!!! ACACTTTTTTCATGTAATGG+AGG + chr6.3:64500078-64500097 None:intergenic 30.0%
!!! ATCAAGATTTTGGTTGATCA+TGG + chr6.3:64499746-64499765 None:intergenic 30.0%
!!! CCTACACTTTTTTCATGTAA+TGG + chr6.3:64500081-64500100 None:intergenic 30.0%
!!! GTTGTGCTATTTTGGAATTT+TGG + chr6.3:64499393-64499412 None:intergenic 30.0%
!!! TTTTCGAAGTTGTGCTATTT+TGG + chr6.3:64499401-64499420 None:intergenic 30.0%
AAAGTGTAGGGAACATACTT+TGG - chr6.3:64500091-64500110 MS.gene068439:CDS 35.0%
AAGAGAGTGGATAAAGTTTG+AGG + chr6.3:64499300-64499319 None:intergenic 35.0%
ATGTTGTCTATGAAAGAGAG+TGG + chr6.3:64499313-64499332 None:intergenic 35.0%
CCTCCATCAAGTAAACTAAA+GGG + chr6.3:64499796-64499815 None:intergenic 35.0%
CTTTCCATTGCAATTCATCA+GGG + chr6.3:64499591-64499610 None:intergenic 35.0%
GATTAAGTCTCTTAAGGCAA+TGG - chr6.3:64499918-64499937 MS.gene068439:CDS 35.0%
GGAAATGGAAATAGAGGATT+TGG - chr6.3:64500140-64500159 MS.gene068439:CDS 35.0%
GGTAAAGAGAGAAAACAATG+TGG - chr6.3:64499864-64499883 MS.gene068439:CDS 35.0%
GTCTATGTTGGCAAATAAGA+AGG - chr6.3:64500119-64500138 MS.gene068439:CDS 35.0%
GTTGGCAAATAAGAAGGAAA+TGG - chr6.3:64500125-64500144 MS.gene068439:CDS 35.0%
TCCTCCATCAAGTAAACTAA+AGG + chr6.3:64499797-64499816 None:intergenic 35.0%
TCTTTCCATTGCAATTCATC+AGG + chr6.3:64499592-64499611 None:intergenic 35.0%
TGAATTGCAATGGAAAGACA+TGG - chr6.3:64499594-64499613 MS.gene068439:CDS 35.0%
TGCAGTGATTAAGTCTCTTA+AGG - chr6.3:64499912-64499931 MS.gene068439:CDS 35.0%
TTAAGAGACTTAATCACTGC+AGG + chr6.3:64499913-64499932 None:intergenic 35.0%
TTCAATGTTTAGCATGGTTG+TGG - chr6.3:64500032-64500051 MS.gene068439:CDS 35.0%
! AAGCACGCTGTTTCAATAAT+GGG - chr6.3:64499994-64500013 MS.gene068439:CDS 35.0%
! ACAGATTTTCAGTGGGATTT+TGG + chr6.3:64499555-64499574 None:intergenic 35.0%
! AGCGTCAATTTTTCCACAAA+GGG - chr6.3:64499816-64499835 MS.gene068439:CDS 35.0%
! ATAAACTGTTTTGCATGTGG+AGG - chr6.3:64499646-64499665 MS.gene068439:CDS 35.0%
! TCACCCTTTAGTTTACTTGA+TGG - chr6.3:64499790-64499809 MS.gene068439:CDS 35.0%
! TTTGGTCTCTACTCTTGTTT+GGG + chr6.3:64499375-64499394 None:intergenic 35.0%
! TTTTGGTCTCTACTCTTGTT+TGG + chr6.3:64499376-64499395 None:intergenic 35.0%
!!! ACTTTGGTTTTCGTCTATGT+TGG - chr6.3:64500107-64500126 MS.gene068439:CDS 35.0%
!!! GGTGTTATTTTTAGTCACAG+AGG - chr6.3:64499504-64499523 MS.gene068439:CDS 35.0%
AAAATGGGCAAATCCCTTTG+TGG + chr6.3:64499832-64499851 None:intergenic 40.0%
ACCATTGAATGCAATGAGCA+AGG - chr6.3:64499481-64499500 MS.gene068439:CDS 40.0%
AGTTACAACTTGTACACGTG+TGG + chr6.3:64499962-64499981 None:intergenic 40.0%
ATGGATACAAGAGATGGAGA+TGG - chr6.3:64500405-64500424 MS.gene068439:CDS 40.0%
CATTGAATGCAATGAGCAAG+GGG - chr6.3:64499483-64499502 MS.gene068439:CDS 40.0%
CCAAAATCCGCTTCATACAT+TGG + chr6.3:64500324-64500343 None:intergenic 40.0%
CCAGACTTCAATGTTTAGCA+TGG - chr6.3:64500026-64500045 MS.gene068439:CDS 40.0%
CCATGCTAAACATTGAAGTC+TGG + chr6.3:64500029-64500048 None:intergenic 40.0%
CCATTGAATGCAATGAGCAA+GGG - chr6.3:64499482-64499501 MS.gene068439:CDS 40.0%
CCTTACAGAACAACCAAAAG+TGG + chr6.3:64500370-64500389 None:intergenic 40.0%
CTCTCTTTACCACAATCTCT+GGG + chr6.3:64499855-64499874 None:intergenic 40.0%
GATTGAAGCGATTGTGAACA+TGG - chr6.3:64500428-64500447 MS.gene068439:CDS 40.0%
GCAATTCATCAGGGAACAAA+GGG + chr6.3:64499582-64499601 None:intergenic 40.0%
GTTACAACTTGTACACGTGT+GGG + chr6.3:64499961-64499980 None:intergenic 40.0%
TCGGTTTCCAATGTATGAAG+CGG - chr6.3:64500314-64500333 MS.gene068439:CDS 40.0%
TCTCTCTTTACCACAATCTC+TGG + chr6.3:64499856-64499875 None:intergenic 40.0%
TGCAATGGAAAGACATGGAT+TGG - chr6.3:64499599-64499618 MS.gene068439:CDS 40.0%
TGCAATTCATCAGGGAACAA+AGG + chr6.3:64499583-64499602 None:intergenic 40.0%
TGGATACAAGAGATGGAGAT+GGG - chr6.3:64500406-64500425 MS.gene068439:CDS 40.0%
TGTTCCCTGATGAATTGCAA+TGG - chr6.3:64499584-64499603 MS.gene068439:CDS 40.0%
! ACCACAATCTCTGGGAAAAT+GGG + chr6.3:64499847-64499866 None:intergenic 40.0%
! AGCACGCTGTTTCAATAATG+GGG - chr6.3:64499995-64500014 MS.gene068439:CDS 40.0%
! AGGGTTCAACAGATTTTCAG+TGG + chr6.3:64499563-64499582 None:intergenic 40.0%
! CAAGCACGCTGTTTCAATAA+TGG - chr6.3:64499993-64500012 MS.gene068439:CDS 40.0%
! CCCTTTAGTTTACTTGATGG+AGG - chr6.3:64499793-64499812 MS.gene068439:CDS 40.0%
! GAGCGTCAATTTTTCCACAA+AGG - chr6.3:64499815-64499834 MS.gene068439:CDS 40.0%
! GGATAAAGTTTGAGGTGAGA+AGG + chr6.3:64499292-64499311 None:intergenic 40.0%
! GGGTTCAACAGATTTTCAGT+GGG + chr6.3:64499562-64499581 None:intergenic 40.0%
! TACCACAATCTCTGGGAAAA+TGG + chr6.3:64499848-64499867 None:intergenic 40.0%
!! CCAATGTATGAAGCGGATTT+TGG - chr6.3:64500321-64500340 MS.gene068439:CDS 40.0%
!! CCACTTTTGGTTGTTCTGTA+AGG - chr6.3:64500367-64500386 MS.gene068439:CDS 40.0%
!! GTATGAAGCGGATTTTGGAT+GGG - chr6.3:64500326-64500345 MS.gene068439:CDS 40.0%
!! TATGAAGCGGATTTTGGATG+GGG - chr6.3:64500327-64500346 MS.gene068439:CDS 40.0%
!! TGTATGAAGCGGATTTTGGA+TGG - chr6.3:64500325-64500344 MS.gene068439:CDS 40.0%
CCCTTGCTCATTGCATTCAA+TGG + chr6.3:64499485-64499504 None:intergenic 45.0%
GTGTACAAGTTGTAACTGCG+TGG - chr6.3:64499965-64499984 MS.gene068439:CDS 45.0%
TGGATGGGGAAAACCAACAT+GGG - chr6.3:64500341-64500360 MS.gene068439:CDS 45.0%
TGTGGATCTTCGCAGAAGAA+TGG - chr6.3:64500050-64500069 MS.gene068439:CDS 45.0%
TTGGATGGGGAAAACCAACA+TGG - chr6.3:64500340-64500359 MS.gene068439:CDS 45.0%
! CTGTTTTGCATGTGGAGGAA+TGG - chr6.3:64499651-64499670 MS.gene068439:CDS 45.0%
!! CGTTGGTGATGAAGGTTTGA+TGG + chr6.3:64499269-64499288 None:intergenic 45.0%
CCAAAAGTGGTCACCCATGT+TGG + chr6.3:64500357-64500376 None:intergenic 50.0%
! AGTTTGAGGTGAGAAGGCGT+TGG + chr6.3:64499286-64499305 None:intergenic 50.0%
! CCAACATGGGTGACCACTTT+TGG - chr6.3:64500354-64500373 MS.gene068439:CDS 50.0%
! GCCCATTTTCCCAGAGATTG+TGG - chr6.3:64499843-64499862 MS.gene068439:CDS 50.0%
!! TGAGAAGGCGTTGGTGATGA+AGG + chr6.3:64499277-64499296 None:intergenic 50.0%


Chromosome Type Strat End Strand Name
chr6.3 gene 64499238 64500527 64499238 ID=MS.gene068439
chr6.3 mRNA 64499238 64500527 64499238 ID=MS.gene068439.t1;Parent=MS.gene068439
chr6.3 exon 64499238 64500527 64499238 ID=MS.gene068439.t1.exon1;Parent=MS.gene068439.t1
chr6.3 CDS 64499238 64500527 64499238 ID=cds.MS.gene068439.t1;Parent=MS.gene068439.t1
Gene Sequence

>MS.gene068439

ATGGAAATAGAATTACTTTCTAGAGAAACCATCAAACCTTCATCACCAACGCCTTCTCACCTCAAACTTTATCCACTCTCTTTCATAGACAACATTGTTTCCCGTAACTATGTGTCATTACTTTACTTTTACAACCCAAACAAGAGTAGAGACCAAAATTCCAAAATAGCACAACTTCGAAAATCTTTATCTCAACTTTTATCTAAATACTATCATTTTGCTGGTAGGATTAAAGATAAAATTACCATTGAATGCAATGAGCAAGGGGTGTTATTTTTAGTCACAGAGGTAAAAAACAAACTTTCAGATATTCTCCAAAATCCCACTGAAAATCTGTTGAACCCTTTGTTCCCTGATGAATTGCAATGGAAAGACATGGATTGGAGTGCAACCTTTATAGCCATTCAAATAAACTGTTTTGCATGTGGAGGAATGGCAATAAGTATTTGCATGAGTCACAAAATTGGTGATGCTTCAACCATTTTCAACTTCATGAATGATTGGGCCATGATCAACCAAAATCTTGATGAAGATGGAAAAGAGTTATTAGTATCACCCTTTAGTTTACTTGATGGAGGAGCGTCAATTTTTCCACAAAGGGATTTGCCCATTTTCCCAGAGATTGTGGTAAAGAGAGAAAACAATGTGGTATTTAAAAGGTTTGTTTTTCATCCTGCAGTGATTAAGTCTCTTAAGGCAATGGTAACTTCTATGCATTCTCCCACACGTGTACAAGTTGTAACTGCGTGGATTTACAAGCACGCTGTTTCAATAATGGGGTTAAATTTCCAGACTTCAATGTTTAGCATGGTTGTGGATCTTCGCAGAAGAATGGTTCCTCCATTACATGAAAAAAGTGTAGGGAACATACTTTGGTTTTCGTCTATGTTGGCAAATAAGAAGGAAATGGAAATAGAGGATTTGGTCTGTCAAATAAAAGAAGGGTTATCTGAGTGTTGTGATGTTTATCCAAAATTATTTAGAGAAGAAGAAAATGATAAGATATCTGAGTGCTTGAAGCAAGTTACACAGCCATATTCTGAGAATAAAAGTTTGTTTACTTTTAGTAGTTGGTGTCGGTTTCCAATGTATGAAGCGGATTTTGGATGGGGAAAACCAACATGGGTGACCACTTTTGGTTGTTCTGTAAGGAATATTGTAATTTTGATGGATACAAGAGATGGAGATGGGATTGAAGCGATTGTGAACATGGAAGATAATTATATGGCTAAGTTCGAACGTGATTTTGAGCTTCTTCGATATGCCTCTCTTAATCCAAACAATGTTTAG
Protein sequence

>MS.gene068439.t1

MEIELLSRETIKPSSPTPSHLKLYPLSFIDNIVSRNYVSLLYFYNPNKSRDQNSKIAQLRKSLSQLLSKYYHFAGRIKDKITIECNEQGVLFLVTEVKNKLSDILQNPTENLLNPLFPDELQWKDMDWSATFIAIQINCFACGGMAISICMSHKIGDASTIFNFMNDWAMINQNLDEDGKELLVSPFSLLDGGASIFPQRDLPIFPEIVVKRENNVVFKRFVFHPAVIKSLKAMVTSMHSPTRVQVVTAWIYKHAVSIMGLNFQTSMFSMVVDLRRRMVPPLHEKSVGNILWFSSMLANKKEMEIEDLVCQIKEGLSECCDVYPKLFREEENDKISECLKQVTQPYSENKSLFTFSSWCRFPMYEADFGWGKPTWVTTFGCSVRNIVILMDTRDGDGIEAIVNMEDNYMAKFERDFELLRYASLNPNNV