AlfalfaGEDB | MS.gene059060

M. sativa cultivar XinJiangDaYe / MS.gene059060

KEGG: -
GO: GO:0003674 GO:0004857 GO:0004864 GO:0005488 GO:0006950 GO:0006952 GO:0007165 GO:0008150 GO:0008289 GO:0009738 GO:0009755 GO:0009987 GO:0010427 GO:0019208 GO:0019212 GO:0019840 GO:0019888 GO:0030234 GO:0031406 GO:0033293 GO:0036094 GO:0038023 GO:0042562 GO:0043167 GO:0043168 GO:0043177 GO:0043178 GO:0050789 GO:0050794 GO:0050896 GO:0060089 GO:0065007 GO:0098772
PFAM: PF00407.22

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene059060.t1	XP_013463153.1	96.5	142	5	0	1	142	19	160	5.40E-73	283.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene059060.t1	P17642	51.8	141	63	2	1	141	19	154	3.2e-37	156.0

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene059060.t1	A0A072V725	96.5	142	5	0	1	142	19	160	3.9e-73	283.5

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene052211	MS.gene059060	0.806815	6.85E-50	-1.69E-46
MS.gene05240	MS.gene059060	0.818803	1.62E-52	-1.69E-46
MS.gene054146	MS.gene059060	0.82437	8.38E-54	-1.69E-46
MS.gene059060	MS.gene062636	0.821701	3.51E-53	-1.69E-46
MS.gene059060	MS.gene066417	0.803584	3.25E-49	-1.69E-46
MS.gene059060	MS.gene068172	0.850786	1.36E-60	-1.69E-46
MS.gene059060	MS.gene068439	0.804638	1.96E-49	-1.69E-46
MS.gene059060	MS.gene068449	0.811121	8.20E-51	-1.69E-46
MS.gene059060	MS.gene069482	0.82161	3.68E-53	-1.69E-46
MS.gene059060	MS.gene071858	0.887548	1.29E-72	-1.69E-46
MS.gene059060	MS.gene072293	0.804317	2.29E-49	-1.69E-46
MS.gene059060	MS.gene42005	0.808416	3.13E-50	-1.69E-46
MS.gene059060	MS.gene46369	0.82073	5.88E-53	-1.69E-46
MS.gene059060	MS.gene47719	0.800425	1.45E-48	-1.69E-46
MS.gene059060	MS.gene48021	0.803118	4.06E-49	-1.69E-46
MS.gene059060	MS.gene48023	0.803196	3.91E-49	-1.69E-46
MS.gene059060	MS.gene48885	0.800373	1.49E-48	-1.69E-46
MS.gene059060	MS.gene50279	0.809991	1.44E-50	-1.69E-46
MS.gene059060	MS.gene65833	0.803112	4.07E-49	-1.69E-46
MS.gene059060	MS.gene72929	0.806277	8.90E-50	-1.69E-46
MS.gene059060	MS.gene81503	0.803612	3.21E-49	-1.69E-46
MS.gene059060	MS.gene81504	0.851804	6.99E-61	-1.69E-46
MS.gene059060	MS.gene81505	0.846867	1.66E-59	-1.69E-46
MS.gene059060	MS.gene81506	0.848395	6.30E-60	-1.69E-46
MS.gene059060	MS.gene87450	0.822313	2.53E-53	-1.69E-46
MS.gene059060	MS.gene95804	0.80126	9.79E-49	-1.69E-46
MS.gene059060	MS.gene98480	0.802275	6.06E-49	-1.69E-46
MS.gene059060	MS.gene99062	0.80404	2.61E-49	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene059060.t1	MTR_2g435310	96.479	142	5	0	1	142	19	160	7.22e-99	281
MS.gene059060.t1	MTR_4g120760	74.648	142	36	0	1	142	19	160	6.93e-79	230
MS.gene059060.t1	MTR_4g120760	73.944	142	37	0	1	142	19	160	2.03e-78	229
MS.gene059060.t1	MTR_2g035100	40.580	138	81	1	1	138	19	155	7.92e-34	116
MS.gene059060.t1	MTR_2g035105	39.130	138	83	1	1	138	19	155	2.59e-32	112
MS.gene059060.t1	MTR_2g035150	39.130	138	83	1	1	138	19	155	2.91e-32	112
MS.gene059060.t1	MTR_2g035120	37.681	138	85	1	1	138	19	155	9.00e-32	111
MS.gene059060.t1	MTR_2g035130	37.681	138	85	1	1	138	19	155	9.55e-32	110
MS.gene059060.t1	MTR_6g033450	37.681	138	85	1	1	138	19	155	1.22e-27	100
MS.gene059060.t1	MTR_4g120970	37.681	138	85	1	1	138	19	155	1.22e-27	100
MS.gene059060.t1	MTR_2g035170	35.507	138	88	1	1	138	19	155	2.71e-25	94.4
MS.gene059060.t1	MTR_2g035220	30.435	138	95	1	1	138	19	155	8.04e-24	90.9
MS.gene059060.t1	MTR_2g035320	30.435	138	95	1	1	138	19	155	2.39e-23	89.4
MS.gene059060.t1	MTR_4g120950	35.000	140	87	3	1	138	19	156	4.09e-23	89.0
MS.gene059060.t1	MTR_2g035210	29.710	138	96	1	1	138	19	155	9.30e-23	88.2
MS.gene059060.t1	MTR_2g035190	28.986	138	97	1	1	138	19	155	2.02e-21	84.7
MS.gene059060.t1	MTR_2g035320	31.111	90	62	0	49	138	31	120	9.35e-13	61.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 26 sgRNAs with CRISPR-Local

Find 41 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ACAAGCAAATACAATGCTAT+TGG	0.304763	2.4:-55842562	MS.gene059060:CDS
CAAGCAAATACAATGCTATT+GGG	0.319492	2.4:-55842561	MS.gene059060:CDS
ATGAACTGAGGTAAGAGTTT+TGG	0.327571	2.4:+55843271	None:intergenic
GCTTGAGTCAATTGCTTATG+AGG	0.335592	2.4:-55842629	MS.gene059060:CDS
TTCCAGCTTCTCCATTTCCT+TGG	0.338136	2.4:+55843230	None:intergenic
AGTCAAAGAGGAAGAGATAA+AGG	0.347021	2.4:-55842530	MS.gene059060:CDS
GACTCAAGCTTGTCACCTAA+TGG	0.355452	2.4:+55842643	None:intergenic
TGCTTATGAGGTTAAGTTTG+AGG	0.429764	2.4:-55842617	MS.gene059060:CDS
AGGAAGAGATAAAGGAAGGA+AGG	0.431527	2.4:-55842522	MS.gene059060:CDS
AGCTCCATCACGTATGTTCA+AGG	0.432574	2.4:-55843323	None:intergenic
GTCAACGTAATCCAAGGAAA+TGG	0.442764	2.4:-55843241	MS.gene059060:CDS
AAGTTTGAGGTCATTAATGA+TGG	0.447146	2.4:-55842604	MS.gene059060:CDS
CACAACTTTGCAAATTCCAA+TGG	0.469640	2.4:+55842492	None:intergenic
AGTTGTGGAAGCATACTTGC+TGG	0.476134	2.4:-55842476	MS.gene059060:CDS
ATCCAAGGAAATGGAGAAGC+TGG	0.480647	2.4:-55843232	MS.gene059060:CDS
ATGATTGAAGGAGATCCATT+AGG	0.495029	2.4:-55842658	MS.gene059060:CDS
CATTGGAATTTGCAAAGTTG+TGG	0.510944	2.4:-55842491	MS.gene059060:CDS
GGAAGAGATAAAGGAAGGAA+GGG	0.516313	2.4:-55842521	MS.gene059060:CDS
TGCAACTACACTATGATTGA+AGG	0.519866	2.4:-55842670	MS.gene059060:CDS
GAAGGAAGGGAAAACTCCAT+TGG	0.523267	2.4:-55842508	MS.gene059060:CDS
AAAGAGGAAGAGATAAAGGA+AGG	0.532049	2.4:-55842526	MS.gene059060:CDS
TATGATTGATAATGATAACT+TGG	0.540241	2.4:-55842695	MS.gene059060:CDS
TTTGAGGTCATTAATGATGG+AGG	0.549468	2.4:-55842601	MS.gene059060:CDS
AAGCAAATACAATGCTATTG+GGG	0.591788	2.4:-55842560	MS.gene059060:CDS
AAAGATGTCAACGTAATCCA+AGG	0.591971	2.4:-55843247	MS.gene059060:CDS
AATAACTAAACATAGACTTG+TGG	0.600652	2.4:+55842448	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	ACCTCAAATAAAAAATATTT+AGG	-	chr2.4:55842795-55842814	MS.gene059060:intron	15.0%
!!!	AAAAAAAAACATTTTAAAGC+TGG	-	chr2.4:55842591-55842610	MS.gene059060:CDS	15.0%
!!!	TTTTCTTTTTTTCTTTATGT+TGG	-	chr2.4:55842662-55842681	MS.gene059060:CDS	15.0%
!!	ACATGAATGATAAAATCTAA+AGG	-	chr2.4:55842825-55842844	MS.gene059060:intron	20.0%
!!	CTTTCAAATATTTGAAAAAC+AGG	-	chr2.4:55843040-55843059	MS.gene059060:intron	20.0%
!!!	ATAAGTAAGAATTGTTATTG+AGG	+	chr2.4:55842993-55843012	None:intergenic	20.0%
!!!	CTGTTTTTCAAATATTTGAA+AGG	+	chr2.4:55843042-55843061	None:intergenic	20.0%
!!!	GCCTAAATATTTTTTATTTG+AGG	+	chr2.4:55842799-55842818	None:intergenic	20.0%
!!!	TATGATTGATAATGATAACT+TGG	-	chr2.4:55843068-55843087	MS.gene059060:intron	20.0%
!	AACCCTAACTAAGATTTATT+TGG	+	chr2.4:55842941-55842960	None:intergenic	25.0%
!	ACCCTAACTAAGATTTATTT+GGG	+	chr2.4:55842940-55842959	None:intergenic	25.0%
	AAGCAAATACAATGCTATTG+GGG	-	chr2.4:55843203-55843222	MS.gene059060:CDS	30.0%
	ACAAGCAAATACAATGCTAT+TGG	-	chr2.4:55843201-55843220	MS.gene059060:intron	30.0%
	CAAGCAAATACAATGCTATT+GGG	-	chr2.4:55843202-55843221	MS.gene059060:intron	30.0%
	GCCCAAATAAATCTTAGTTA+GGG	-	chr2.4:55842936-55842955	MS.gene059060:intron	30.0%
	TGCCCAAATAAATCTTAGTT+AGG	-	chr2.4:55842935-55842954	MS.gene059060:intron	30.0%
!	AAGTTTGAGGTCATTAATGA+TGG	-	chr2.4:55843159-55843178	MS.gene059060:intron	30.0%
!	AATTGTTATTGAGGCAACAT+AGG	+	chr2.4:55842984-55843003	None:intergenic	30.0%
!	GAACAAGTTAATTTTGCTGA+AGG	-	chr2.4:55842561-55842580	MS.gene059060:CDS	30.0%
!	TTGACATCTTTTATGAACTG+AGG	+	chr2.4:55842507-55842526	None:intergenic	30.0%
	AAAGAGGAAGAGATAAAGGA+AGG	-	chr2.4:55843237-55843256	MS.gene059060:CDS	35.0%
	AAAGATGTCAACGTAATCCA+AGG	-	chr2.4:55842516-55842535	MS.gene059060:CDS	35.0%
	AGTCAAAGAGGAAGAGATAA+AGG	-	chr2.4:55843233-55843252	MS.gene059060:CDS	35.0%
	ATGATTGAAGGAGATCCATT+AGG	-	chr2.4:55843105-55843124	MS.gene059060:intron	35.0%
	CACAACTTTGCAAATTCCAA+TGG	+	chr2.4:55843274-55843293	None:intergenic	35.0%
	CATTGGAATTTGCAAAGTTG+TGG	-	chr2.4:55843272-55843291	MS.gene059060:CDS	35.0%
	GTTGCGAGAAATACTTAGTT+TGG	-	chr2.4:55842768-55842787	MS.gene059060:intron	35.0%
	TGCAACTACACTATGATTGA+AGG	-	chr2.4:55843093-55843112	MS.gene059060:intron	35.0%
	TGCTTATGAGGTTAAGTTTG+AGG	-	chr2.4:55843146-55843165	MS.gene059060:intron	35.0%
!	ATGAACTGAGGTAAGAGTTT+TGG	+	chr2.4:55842495-55842514	None:intergenic	35.0%
!	TTTGAGGTCATTAATGATGG+AGG	-	chr2.4:55843162-55843181	MS.gene059060:intron	35.0%
	AGGAAGAGATAAAGGAAGGA+AGG	-	chr2.4:55843241-55843260	MS.gene059060:CDS	40.0%
	GCTTGAGTCAATTGCTTATG+AGG	-	chr2.4:55843134-55843153	MS.gene059060:intron	40.0%
	GGAAGAGATAAAGGAAGGAA+GGG	-	chr2.4:55843242-55843261	MS.gene059060:CDS	40.0%
	GTCAACGTAATCCAAGGAAA+TGG	-	chr2.4:55842522-55842541	MS.gene059060:CDS	40.0%
!!!	TGGGGATTTTGAAGTCAAAG+AGG	-	chr2.4:55843221-55843240	MS.gene059060:CDS	40.0%
	ATCCAAGGAAATGGAGAAGC+TGG	-	chr2.4:55842531-55842550	MS.gene059060:CDS	45.0%
	GAAGGAAGGGAAAACTCCAT+TGG	-	chr2.4:55843255-55843274	MS.gene059060:CDS	45.0%
	GACTCAAGCTTGTCACCTAA+TGG	+	chr2.4:55843123-55843142	None:intergenic	45.0%
	TTCCAGCTTCTCCATTTCCT+TGG	+	chr2.4:55842536-55842555	None:intergenic	45.0%
!	AGTTGTGGAAGCATACTTGC+TGG	-	chr2.4:55843287-55843306	MS.gene059060:CDS	45.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.4	gene	55842453	55843332	55842453	ID=MS.gene059060
chr2.4	mRNA	55842453	55843332	55842453	ID=MS.gene059060.t1;Parent=MS.gene059060
chr2.4	exon	55843203	55843332	55843203	ID=MS.gene059060.t1.exon1;Parent=MS.gene059060.t1
chr2.4	CDS	55843203	55843332	55843203	ID=cds.MS.gene059060.t1;Parent=MS.gene059060.t1
chr2.4	exon	55842453	55842751	55842453	ID=MS.gene059060.t1.exon2;Parent=MS.gene059060.t1
chr2.4	CDS	55842453	55842751	55842453	ID=cds.MS.gene059060.t1;Parent=MS.gene059060.t1

Gene Sequence

>MS.gene059060

ATGTTCAAGGCTTTAATCACAGACTCTAGCAATTTGCTTCCAAAACTCTTACCTCAGTTCATAAAAGATGTCAACGTAATCCAAGGAAATGGAGAAGCTGGAACAATTGAACAAGTTAATTTTGCTGAAGGTTAGCAAAAAAAAAAACATTTTAAAGCTGGAAGAATTAAAATGCTCTTTTTATTTTTTATTTTTAAATTTAAAGAGATTTTTCTTTTTTTCTTTATGTTGGTTGAATAACTTCTTACTTACATGTTTCATTTTAGAGAGAAAAAATAGACTTATATTTTTATTGAAAAGTGATTTTTTTTCTAAGTTGCGAGAAATACTTAGTTTGGCACAACCTCAAATAAAAAATATTTAGGCACATACACATGAATGATAAAATCTAAAGGAAAAATAATTTAGTCACATTGCTCTCAAAAAAAAAAAAATTAGTCACATCATTTCTCTTTTAAATTTTCATCAATCATGTGTTTGTGTGCCCAAATAAATCTTAGTTAGGGTTGTGTCAATACAAGACTTGCTCCTATGTTGCCTCAATAACAATTCTTACTTATATGTTCTATTTATCATTGCAGCTAATCCTTTCAAATATTTGAAAAACAGGATTGATATGATTGATAATGATAACTTGGTGTGCAACTACACTATGATTGAAGGAGATCCATTAGGTGACAAGCTTGAGTCAATTGCTTATGAGGTTAAGTTTGAGGTCATTAATGATGGAGGTTGTTTTTGTAAAATGACAAGCAAATACAATGCTATTGGGGATTTTGAAGTCAAAGAGGAAGAGATAAAGGAAGGAAGGGAAAACTCCATTGGAATTTGCAAAGTTGTGGAAGCATACTTGCTGGAGAATCCACAAGTCTATGTTTAG

Protein sequence

>MS.gene059060.t1

MFKALITDSSNLLPKLLPQFIKDVNVIQGNGEAGTIEQVNFAEANPFKYLKNRIDMIDNDNLVCNYTMIEGDPLGDKLESIAYEVKFEVINDGGCFCKMTSKYNAIGDFEVKEEEIKEGRENSIGICKVVEAYLLENPQVYV