Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene068791.t1 | KEH38769.1 | 96.3 | 190 | 7 | 0 | 1 | 190 | 1 | 190 | 6.70E-103 | 383.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene068791.t1 | P24805 | 40.9 | 149 | 87 | 1 | 1 | 149 | 1 | 148 | 2.3e-27 | 123.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene068791.t1 | B7FHV5 | 96.3 | 190 | 7 | 0 | 1 | 190 | 1 | 190 | 4.9e-103 | 383.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene056623 | MS.gene068791 | 0.836527 | 8.88E-57 | -1.69E-46 |
| MS.gene059856 | MS.gene068791 | 0.908617 | 1.41E-81 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene068791.t1 | MTR_2g079670 | 96.316 | 190 | 7 | 0 | 1 | 190 | 1 | 190 | 5.87e-127 | 355 |
| MS.gene068791.t1 | MTR_2g079670 | 96.316 | 190 | 7 | 0 | 1 | 190 | 1 | 190 | 3.65e-126 | 355 |
| MS.gene068791.t1 | MTR_2g013140 | 63.684 | 190 | 68 | 1 | 1 | 190 | 1 | 189 | 6.39e-81 | 239 |
| MS.gene068791.t1 | MTR_2g013140 | 63.684 | 190 | 68 | 1 | 1 | 190 | 1 | 189 | 5.96e-80 | 238 |
| MS.gene068791.t1 | MTR_4g088955 | 60.000 | 190 | 76 | 0 | 1 | 190 | 64 | 253 | 1.76e-78 | 237 |
| MS.gene068791.t1 | MTR_2g079670 | 96.667 | 120 | 4 | 0 | 71 | 190 | 22 | 141 | 3.51e-72 | 217 |
| MS.gene068791.t1 | MTR_1g041380 | 44.503 | 191 | 99 | 3 | 1 | 189 | 1 | 186 | 4.70e-51 | 163 |
| MS.gene068791.t1 | MTR_1g041380 | 44.503 | 191 | 99 | 3 | 1 | 189 | 1 | 186 | 6.92e-50 | 162 |
| MS.gene068791.t1 | MTR_1g102680 | 43.750 | 192 | 99 | 4 | 1 | 189 | 1 | 186 | 2.52e-48 | 158 |
| MS.gene068791.t1 | MTR_2g022520 | 41.885 | 191 | 105 | 4 | 1 | 189 | 1 | 187 | 4.88e-45 | 149 |
| MS.gene068791.t1 | MTR_4g117050 | 38.743 | 191 | 112 | 4 | 1 | 189 | 1 | 188 | 8.44e-44 | 146 |
| MS.gene068791.t1 | MTR_4g117050 | 44.715 | 123 | 68 | 0 | 67 | 189 | 21 | 143 | 3.08e-37 | 127 |
| MS.gene068791.t1 | MTR_1g019625 | 75.926 | 54 | 6 | 1 | 113 | 159 | 14 | 67 | 1.23e-19 | 79.0 |
| MS.gene068791.t1 | MTR_7g059340 | 76.316 | 38 | 9 | 0 | 114 | 151 | 18 | 55 | 3.88e-13 | 62.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene068791.t1 | AT4G27450 | 76.440 | 191 | 44 | 1 | 1 | 190 | 1 | 191 | 9.53e-105 | 301 |
| MS.gene068791.t1 | AT3G15450 | 66.492 | 191 | 60 | 3 | 1 | 189 | 1 | 189 | 5.10e-89 | 260 |
| MS.gene068791.t1 | AT3G15450 | 66.492 | 191 | 60 | 3 | 1 | 189 | 1 | 189 | 1.22e-88 | 260 |
| MS.gene068791.t1 | AT3G15450 | 64.286 | 182 | 58 | 4 | 1 | 180 | 1 | 177 | 6.78e-77 | 228 |
| MS.gene068791.t1 | AT5G43830 | 46.875 | 192 | 94 | 3 | 1 | 189 | 1 | 187 | 3.21e-54 | 173 |
| MS.gene068791.t1 | AT3G22850 | 41.270 | 189 | 108 | 1 | 1 | 189 | 1 | 186 | 5.78e-46 | 152 |
| MS.gene068791.t1 | AT5G19140 | 41.053 | 190 | 108 | 3 | 1 | 189 | 1 | 187 | 2.64e-44 | 147 |
| MS.gene068791.t1 | AT5G19140 | 40.642 | 187 | 106 | 4 | 1 | 186 | 1 | 183 | 1.93e-40 | 137 |
| MS.gene068791.t1 | AT5G19140 | 48.819 | 127 | 65 | 0 | 63 | 189 | 25 | 151 | 3.02e-39 | 133 |
Find 0 sgRNAs with CRISPR-Local
Find 69 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATCAATTTTTCCAATAAAAA+AGG | - | 15113:22248-22267 | MS.gene068791:intergenic | 15.0% |
| !! | TCCAACATAAAAGTTTAATA+CGG | + | 15113:22159-22178 | MS.gene068791:intron | 20.0% |
| !!! | CATCTTTAATTCCTTTTATA+AGG | + | 15113:22126-22145 | MS.gene068791:intron | 20.0% |
| !!! | TTTTAGTACACCTTTTTTTA+TGG | + | 15113:22000-22019 | MS.gene068791:intron | 20.0% |
| ! | AATACTTGTTCTATGAACTT+TGG | + | 15113:21884-21903 | MS.gene068791:CDS | 25.0% |
| ! | AGAATTGTTAACAAAGAACA+TGG | - | 15113:22664-22683 | MS.gene068791:intergenic | 25.0% |
| ! | TTCATAGAACAAGTATTATC+AGG | - | 15113:21881-21900 | MS.gene068791:intergenic | 25.0% |
| !! | ACCGTATTAAACTTTTATGT+TGG | - | 15113:22163-22182 | MS.gene068791:intergenic | 25.0% |
| !! | TCCTTTTATAAGGATGAATT+CGG | + | 15113:22136-22155 | MS.gene068791:intron | 25.0% |
| !!! | GATGTTTGTTTTTTATTGCA+GGG | + | 15113:22710-22729 | MS.gene068791:intron | 25.0% |
| !!! | TGATGTTTGTTTTTTATTGC+AGG | + | 15113:22709-22728 | MS.gene068791:intron | 25.0% |
| AACCTTTGATGAACAGAAAA+AGG | - | 15113:21944-21963 | MS.gene068790:intergenic | 30.0% | |
| ACATTTACTGCTTATTCTTG+GGG | + | 15113:22413-22432 | MS.gene068791:CDS | 30.0% | |
| ACCGAATTCATCCTTATAAA+AGG | - | 15113:22140-22159 | MS.gene068791:intergenic | 30.0% | |
| AGAATTTCTTCAGAAAGCTT+AGG | - | 15113:21836-21855 | MS.gene068791:intergenic | 30.0% | |
| ATAAGCTTCAATCAGAAACA+TGG | - | 15113:22500-22519 | MS.gene068791:intergenic | 30.0% | |
| ATGTTATTAGTGCTAAGAGA+TGG | + | 15113:22359-22378 | MS.gene068791:intron | 30.0% | |
| ATTGATGTTACTGACCAAAT+TGG | + | 15113:22262-22281 | MS.gene068791:intron | 30.0% | |
| CAAAGACGTGTATTATGTTA+CGG | + | 15113:22203-22222 | MS.gene068791:intron | 30.0% | |
| GACATTTACTGCTTATTCTT+GGG | + | 15113:22412-22431 | MS.gene068791:CDS | 30.0% | |
| TGACATTTACTGCTTATTCT+TGG | + | 15113:22411-22430 | MS.gene068791:CDS | 30.0% | |
| TTCGTAGCAACCATAAAAAA+AGG | - | 15113:22013-22032 | MS.gene068791:intergenic | 30.0% | |
| ! | AAGCAATATGGTTTGTCAAA+GGG | + | 15113:22463-22482 | MS.gene068791:CDS | 30.0% |
| ! | TGTTTCTGATTGAAGCTTAT+AGG | + | 15113:22500-22519 | MS.gene068791:CDS | 30.0% |
| ! | TTTTGTAGCAACCATGTTAT+TGG | - | 15113:22320-22339 | MS.gene068791:intergenic | 30.0% |
| !! | ATTTTTGCAGGTTGTTTTGT+GGG | + | 15113:22383-22402 | MS.gene068791:intron | 30.0% |
| !! | TATTTTTGCAGGTTGTTTTG+TGG | + | 15113:22382-22401 | MS.gene068791:intron | 30.0% |
| !!! | CAGTTGCATACCTTTTTTAT+TGG | + | 15113:22235-22254 | MS.gene068791:intron | 30.0% |
| AACCCTCTTGTACAACATTA+AGG | - | 15113:22802-22821 | MS.gene068790:intergenic | 35.0% | |
| ACCATATTGCTTGTTCAGTA+AGG | - | 15113:22455-22474 | MS.gene068791:intergenic | 35.0% | |
| AGTTCCTTAACAACTTGATC+TGG | - | 15113:22553-22572 | MS.gene068791:intergenic | 35.0% | |
| CAAAGAATCGCAATCATATG+TGG | + | 15113:22078-22097 | MS.gene068791:intron | 35.0% | |
| CAAGTTGTTAAGGAACTTGA+TGG | + | 15113:22556-22575 | MS.gene068791:CDS | 35.0% | |
| CGTAACATAATACACGTCTT+TGG | - | 15113:22205-22224 | MS.gene068791:intergenic | 35.0% | |
| GACCTTAATGTTGTACAAGA+GGG | + | 15113:22797-22816 | MS.gene068791:CDS | 35.0% | |
| GTTGTTTATGACAGCACATA+TGG | + | 15113:22592-22611 | MS.gene068791:CDS | 35.0% | |
| TCCTTACTGAACAAGCAATA+TGG | + | 15113:22451-22470 | MS.gene068791:CDS | 35.0% | |
| TGAACAGAAAAAGGCTTATC+AGG | - | 15113:21935-21954 | MS.gene068791:intergenic | 35.0% | |
| TGACCTTAATGTTGTACAAG+AGG | + | 15113:22796-22815 | MS.gene068791:CDS | 35.0% | |
| TGTGCTGTCATAAACAACAA+AGG | - | 15113:22590-22609 | MS.gene068791:intergenic | 35.0% | |
| TTAAGCCTTTGTATGATGCA+GGG | - | 15113:21808-21827 | MS.gene068791:intergenic | 35.0% | |
| TTTAAGCCTTTGTATGATGC+AGG | - | 15113:21809-21828 | MS.gene068791:intergenic | 35.0% | |
| TTTAATACGGTATCAGAGTC+TGG | + | 15113:22172-22191 | MS.gene068791:intron | 35.0% | |
| ! | AGCCTTTTTCTGTTCATCAA+AGG | + | 15113:21939-21958 | MS.gene068791:CDS | 35.0% |
| ! | ATGTTGGCAATTTTCCACAA+AGG | + | 15113:21752-21771 | MS.gene068791:CDS | 35.0% |
| ! | CAAGCAATATGGTTTGTCAA+AGG | + | 15113:22462-22481 | MS.gene068791:CDS | 35.0% |
| !! | ATGGTGGATTGAAGTTGTAT+TGG | + | 15113:22738-22757 | MS.gene068791:CDS | 35.0% |
| !! | CTAAGAGATGGTATTTTTGC+AGG | + | 15113:22371-22390 | MS.gene068791:intron | 35.0% |
| !! | TGGTGGATTGAAGTTGTATT+GGG | + | 15113:22739-22758 | MS.gene068791:CDS | 35.0% |
| !! | TTTTTATTGCAGGGTTCTGA+TGG | + | 15113:22719-22738 | MS.gene068791:intron | 35.0% |
| AATAGCCCTGCATCATACAA+AGG | + | 15113:21800-21819 | MS.gene068791:CDS | 40.0% | |
| ATTTGCTTGCGCCAATAACA+TGG | + | 15113:22306-22325 | MS.gene068791:intron | 40.0% | |
| GATGTTACTGACCAAATTGG+CGG | + | 15113:22265-22284 | MS.gene068791:intron | 40.0% | |
| TCCTTAACAACTTGATCTGG+TGG | - | 15113:22550-22569 | MS.gene068791:intergenic | 40.0% | |
| ! | ACAACTTGATCTGGTGGATA+TGG | - | 15113:22544-22563 | MS.gene068791:intergenic | 40.0% |
| ! | TCCACCAGATCAAGTTGTTA+AGG | + | 15113:22546-22565 | MS.gene068791:CDS | 40.0% |
| !! | GGTGGATTGAAGTTGTATTG+GGG | + | 15113:22740-22759 | MS.gene068791:CDS | 40.0% |
| !!! | TGGAATTGTTTTTGCTGCAC+TGG | + | 15113:22612-22631 | MS.gene068791:CDS | 40.0% |
| ! | TTATTGCAGGGTTCTGATGG+TGG | + | 15113:22722-22741 | MS.gene068791:intron | 45.0% |
| !! | AGTACTGATCTCCGCCAATT+TGG | - | 15113:22279-22298 | MS.gene068791:intergenic | 45.0% |
| GATCACAACAGACCCATCAG+CGG | - | 15113:22771-22790 | MS.gene068791:intergenic | 50.0% | |
| GTGGATGAGCAAGACCTTTG+TGG | - | 15113:21769-21788 | MS.gene068791:intergenic | 50.0% | |
| ! | GATCTGGTGGATATGGACCA+CGG | - | 15113:22537-22556 | MS.gene068791:intergenic | 50.0% |
| !! | ATTGGGGTATTGCCGCTGAT+GGG | + | 15113:22756-22775 | MS.gene068791:CDS | 50.0% |
| !! | TATTGGGGTATTGCCGCTGA+TGG | + | 15113:22755-22774 | MS.gene068791:CDS | 50.0% |
| GCAGGGCTATTCAGCTCTTC+AGG | - | 15113:21791-21810 | MS.gene068791:intergenic | 55.0% | |
| GGGCTATTCAGCTCTTCAGG+TGG | - | 15113:21788-21807 | MS.gene068791:intergenic | 55.0% | |
| GTGGATATGGACCACGGTCA+CGG | - | 15113:22531-22550 | MS.gene068791:intergenic | 55.0% | |
| TATAGGACACTCCGTGACCG+TGG | + | 15113:22517-22536 | MS.gene068791:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 15113 | gene | 21752 | 22851 | 21752 | ID=MS.gene068791 |
| 15113 | mRNA | 21752 | 22851 | 21752 | ID=MS.gene068791.t1;Parent=MS.gene068791 |
| 15113 | exon | 21752 | 21960 | 21752 | ID=MS.gene068791.t1.exon1;Parent=MS.gene068791.t1 |
| 15113 | CDS | 21752 | 21960 | 21752 | ID=cds.MS.gene068791.t1;Parent=MS.gene068791.t1 |
| 15113 | exon | 22393 | 22633 | 22393 | ID=MS.gene068791.t1.exon2;Parent=MS.gene068791.t1 |
| 15113 | CDS | 22393 | 22633 | 22393 | ID=cds.MS.gene068791.t1;Parent=MS.gene068791.t1 |
| 15113 | exon | 22731 | 22851 | 22731 | ID=MS.gene068791.t1.exon3;Parent=MS.gene068791.t1 |
| 15113 | CDS | 22731 | 22851 | 22731 | ID=cds.MS.gene068791.t1;Parent=MS.gene068791.t1 |
| Gene Sequence |
| Protein sequence |
