AlfalfaGEDB | MS.gene056623

M. sativa cultivar XinJiangDaYe / MS.gene056623

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene056623.t1	AES89058.1	90.7	75	6	1	1	75	1	74	2.40E-27	131

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene056623.t1	G7JF00	90.7	75	6	1	1	75	1	74	1.7e-27	131.0

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene056623	MS.gene059856	0.816209	6.22E-52	-1.69E-46
MS.gene056623	MS.gene068791	0.836527	8.88E-57	-1.69E-46
MS.gene056623	MS.gene068792	0.850572	1.56E-60	-1.69E-46
MS.gene056623	MS.gene069368	0.852006	6.13E-61	-1.69E-46
MS.gene056623	MS.gene069369	0.845163	4.82E-59	-1.69E-46
MS.gene056623	MS.gene32779	0.834777	2.46E-56	-1.69E-46
MS.gene056623	MS.gene37400	0.805909	1.06E-49	-1.69E-46
MS.gene056623	MS.gene40636	0.976909	9.79E-143	-1.69E-46
MS.gene056623	MS.gene46201	0.963259	7.23E-122	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene056623.t1	MTR_4g068560	90.667	75	6	1	1	75	1	74	2.48e-43	134
MS.gene056623.t1	MTR_4g068453	70.667	75	18	2	1	75	1	71	9.65e-30	100
MS.gene056623.t1	MTR_1g034290	52.000	75	31	3	1	74	1	71	9.93e-19	72.8
MS.gene056623.t1	MTR_1g034270	44.595	74	33	1	1	74	1	66	1.24e-16	67.4

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene056623.t1	AT2G15960	40.244	82	37	2	1	75	1	77	8.48e-14	60.5

Find 20 sgRNAs with CRISPR-Local

Find 21 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GGCAACCGTGTGTTTGCTTA+TGG	0.279387	4.3:-36587862	MS.gene056623:CDS
CCATTGTAGAGGTATGGGTT+GGG	0.390310	4.3:-36588040	MS.gene056623:CDS
AGGCAACAGAGGTGTCTCTT+GGG	0.412772	4.3:+36587907	None:intergenic
TTATGGATCGATTTGTTCCT+TGG	0.422668	4.3:-36587845	MS.gene056623:CDS
TGGCTGCCATTGTAGAGGTA+TGG	0.433651	4.3:-36588046	MS.gene056623:CDS
GAGGCAACAGAGGTGTCTCT+TGG	0.459401	4.3:+36587906	None:intergenic
CATTGTAGAGGTATGGGTTG+GGG	0.470237	4.3:-36588039	MS.gene056623:CDS
GCCATTGTAGAGGTATGGGT+TGG	0.477069	4.3:-36588041	MS.gene056623:CDS
GGCAACAGAGGTGTCTCTTG+GGG	0.546169	4.3:+36587908	None:intergenic
GGCTGCCATTGTAGAGGTAT+GGG	0.559523	4.3:-36588045	MS.gene056623:CDS
AACAAAGGCTTGCTGTTGCT+AGG	0.594023	4.3:+36587987	None:intergenic
TGAAGAGTCAGAGGCAACAG+AGG	0.597712	4.3:+36587896	None:intergenic
ATTAATGGCTGCCATTGTAG+AGG	0.614505	4.3:-36588051	None:intergenic
TGACATAGTTGAAGAGTCAG+AGG	0.626699	4.3:+36587887	None:intergenic
CCCAACCCATACCTCTACAA+TGG	0.629283	4.3:+36588040	None:intergenic
CAAAACAAGAAGAAGAACGT+TGG	0.651156	4.3:-36587939	MS.gene056623:CDS
TGACTCTTCAACTATGTCAG+AGG	0.654740	4.3:-36587883	MS.gene056623:CDS
GCAACAGAGGTGTCTCTTGG+GGG	0.674229	4.3:+36587909	None:intergenic
ATTAACATATACTATCACCA+AGG	0.710327	4.3:+36587828	None:intergenic
TCGATCCATAAGCAAACACA+CGG	0.710548	4.3:+36587857	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	TTGTTTTCAAAAGCCAAACA+AGG	-	chr4.3:36587920-36587939	MS.gene056623:CDS	30.0%
!!!	TGTTTGGCTTTTGAAAACAA+AGG	+	chr4.3:36587920-36587939	None:intergenic	30.0%
	CAAAACAAGAAGAAGAACGT+TGG	-	chr4.3:36587950-36587969	MS.gene056623:CDS	35.0%
!!	TTATGGATCGATTTGTTCCT+TGG	-	chr4.3:36588044-36588063	MS.gene056623:CDS	35.0%
	TCGATCCATAAGCAAACACA+CGG	+	chr4.3:36588035-36588054	None:intergenic	40.0%
	TGACATAGTTGAAGAGTCAG+AGG	+	chr4.3:36588005-36588024	None:intergenic	40.0%
	TGACTCTTCAACTATGTCAG+AGG	-	chr4.3:36588006-36588025	MS.gene056623:CDS	40.0%
!	CATTGTAGAGGTATGGGTTG+GGG	-	chr4.3:36587850-36587869	MS.gene056623:CDS	45.0%
!	CCATTGTAGAGGTATGGGTT+GGG	-	chr4.3:36587849-36587868	MS.gene056623:CDS	45.0%
!!	AACAAAGGCTTGCTGTTGCT+AGG	+	chr4.3:36587905-36587924	None:intergenic	45.0%
!!	GTTTTGATCTGCGCCTTGTT+TGG	+	chr4.3:36587936-36587955	None:intergenic	45.0%
	CCCAACCCATACCTCTACAA+TGG	+	chr4.3:36587852-36587871	None:intergenic	50.0%
	GGCAACCGTGTGTTTGCTTA+TGG	-	chr4.3:36588027-36588046	MS.gene056623:CDS	50.0%
	TGAAGAGTCAGAGGCAACAG+AGG	+	chr4.3:36587996-36588015	None:intergenic	50.0%
	TGGCTGCCATTGTAGAGGTA+TGG	-	chr4.3:36587843-36587862	MS.gene056623:CDS	50.0%
!	GCCATTGTAGAGGTATGGGT+TGG	-	chr4.3:36587848-36587867	MS.gene056623:CDS	50.0%
!	GGCTGCCATTGTAGAGGTAT+GGG	-	chr4.3:36587844-36587863	MS.gene056623:CDS	50.0%
!!	AGGCAACAGAGGTGTCTCTT+GGG	+	chr4.3:36587985-36588004	None:intergenic	50.0%
!!	GAGGCAACAGAGGTGTCTCT+TGG	+	chr4.3:36587986-36588005	None:intergenic	55.0%
!!	GCAACAGAGGTGTCTCTTGG+GGG	+	chr4.3:36587983-36588002	None:intergenic	55.0%
!!	GGCAACAGAGGTGTCTCTTG+GGG	+	chr4.3:36587984-36588003	None:intergenic	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.3	gene	36587842	36588069	36587842	ID=MS.gene056623
chr4.3	mRNA	36587842	36588069	36587842	ID=MS.gene056623.t1;Parent=MS.gene056623
chr4.3	exon	36587842	36588069	36587842	ID=MS.gene056623.t1.exon1;Parent=MS.gene056623.t1
chr4.3	CDS	36587842	36588069	36587842	ID=cds.MS.gene056623.t1;Parent=MS.gene056623.t1

Gene Sequence	>MS.gene056623 ATGGCTGCCATTGTAGAGGTATGGGTTGGGGAGCTAACAAAGCTCAGAGAAAAAGTTCTTCCTAGCAACAGCAAGCCTTTGTTTTCAAAAGCCAAACAAGGCGCAGATCAAAACAAGAAGAAGAACGTTGGTGTTCTTCCCCCAAGAGACACCTCTGTTGCCTCTGACTCTTCAACTATGTCAGAGGCAACCGTGTGTTTGCTTATGGATCGATTTGTTCCTTGGTGA
Protein sequence	>MS.gene056623.t1 MAAIVEVWVGELTKLREKVLPSNSKPLFSKAKQGADQNKKKNVGVLPPRDTSVASDSSTMSEATVCLLMDRFVPW