Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene068825.t1 | XP_013453887.1 | 96 | 253 | 8 | 1 | 1 | 251 | 1 | 253 | 1.40E-127 | 465.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene068825.t1 | A0A072UFN7 | 96.0 | 253 | 8 | 1 | 1 | 251 | 1 | 253 | 9.8e-128 | 465.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene053959 | MS.gene068825 | 0.923558 | 2.27E-89 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene068825.t1 | MTR_5g049260 | 96.047 | 253 | 8 | 1 | 1 | 251 | 1 | 253 | 7.28e-175 | 481 |
| MS.gene068825.t1 | MTR_5g049270 | 96.047 | 253 | 8 | 1 | 1 | 251 | 1 | 253 | 7.28e-175 | 481 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene068825.t1 | AT3G25545 | 48.936 | 235 | 105 | 5 | 1 | 222 | 1 | 233 | 5.38e-62 | 196 |
Find 0 sgRNAs with CRISPR-Local
Find 94 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATGATGAGGTTTTCAAATTT+GGG | - | 37916:11761-11780 | MS.gene068825:CDS | 25.0% |
| !!! | GTGTCAGATTTTGAAAATTA+GGG | - | 37916:11639-11658 | MS.gene068825:CDS | 25.0% |
| AAGATGGTGTGAAATTGTTT+AGG | + | 37916:11126-11145 | MS.gene068824:intergenic | 30.0% | |
| ACAACATCTGAATCAAATTG+AGG | + | 37916:11685-11704 | MS.gene068824:intergenic | 30.0% | |
| TCTTCTTCTAGTAATCTTGA+TGG | + | 37916:11103-11122 | MS.gene068825:intergenic | 30.0% | |
| ! | GATGATGAGGTTTTCAAATT+TGG | - | 37916:11760-11779 | MS.gene068825:CDS | 30.0% |
| !! | GGTGTCAGATTTTGAAAATT+AGG | - | 37916:11638-11657 | MS.gene068825:CDS | 30.0% |
| !!! | AGATGTTGTTTTGTTGTTGA+AGG | - | 37916:11696-11715 | MS.gene068825:CDS | 30.0% |
| CAAGTATGATAACCGAATCA+GGG | + | 37916:11444-11463 | MS.gene068825:intergenic | 35.0% | |
| TCAAGTATGATAACCGAATC+AGG | + | 37916:11445-11464 | MS.gene068825:intergenic | 35.0% | |
| TCTTCTAGTAATCTTGATGG+TGG | + | 37916:11100-11119 | MS.gene068825:intergenic | 35.0% | |
| TTTCAAATTTGGGAGGAGTA+AGG | - | 37916:11771-11790 | MS.gene068825:CDS | 35.0% | |
| ! | ATGAGGTTTTCAAATTTGGG+AGG | - | 37916:11764-11783 | MS.gene068825:CDS | 35.0% |
| ! | TTTTGCTGAAAGGAAGAAAG+CGG | - | 37916:11534-11553 | MS.gene068825:CDS | 35.0% |
| GATGAGGAGAAATTGTTGAG+TGG | - | 37916:11556-11575 | MS.gene068825:CDS | 40.0% | |
| GGAAAAGGAGAAAAGGTGAA+GGG | + | 37916:11159-11178 | MS.gene068825:intergenic | 40.0% | |
| GGAGAAATTGTTGAGTGGAT+TGG | - | 37916:11561-11580 | MS.gene068825:CDS | 40.0% | |
| ! | AAGGATTTGCTTCGAGGTTT+GGG | - | 37916:11715-11734 | MS.gene068825:CDS | 40.0% |
| ! | GTTTGGGTGAGTTTGAGTTT+GGG | - | 37916:11731-11750 | MS.gene068825:CDS | 40.0% |
| ! | GTTTGGGTTTAGTGATGATG+AGG | - | 37916:11747-11766 | MS.gene068825:CDS | 40.0% |
| ! | TTGTTGAAGGATTTGCTTCG+AGG | - | 37916:11709-11728 | MS.gene068825:CDS | 40.0% |
| !! | GTTGATGGTGTTGTTAGGAT+GGG | + | 37916:11317-11336 | MS.gene068825:intergenic | 40.0% |
| !! | TGGATGTTGATGGTGTTGTT+AGG | + | 37916:11322-11341 | MS.gene068825:intergenic | 40.0% |
| !! | TGTTGATGGTGTTGTTAGGA+TGG | + | 37916:11318-11337 | MS.gene068825:intergenic | 40.0% |
| !!! | ATCAGGGATAGGGTTTTGAA+CGG | + | 37916:11374-11393 | MS.gene068825:intergenic | 40.0% |
| AAGCGCGATTATCAGGGATA+GGG | + | 37916:11384-11403 | MS.gene068824:intergenic | 45.0% | |
| AATCTGACACCGTCCAAGAA+TGG | + | 37916:11629-11648 | MS.gene068824:intergenic | 45.0% | |
| AGAAAAGGTGAAGGGAGTGA+TGG | + | 37916:11151-11170 | MS.gene068824:intergenic | 45.0% | |
| AGAGAGAGAGTGGATGTTGA+TGG | + | 37916:11332-11351 | MS.gene068824:intergenic | 45.0% | |
| GAGAAGAGATAGCGATGATG+AGG | + | 37916:11216-11235 | MS.gene068825:intergenic | 45.0% | |
| GGGAAAAGGAGAAAAGGTGA+AGG | + | 37916:11160-11179 | MS.gene068825:intergenic | 45.0% | |
| GTATGATAACCGAATCAGGG+CGG | + | 37916:11441-11460 | MS.gene068825:intergenic | 45.0% | |
| GTGTGAGGGAAAAGGAGAAA+AGG | + | 37916:11166-11185 | MS.gene068825:intergenic | 45.0% | |
| GTTGTTGTGTGTGAGGGAAA+AGG | + | 37916:11174-11193 | MS.gene068825:intergenic | 45.0% | |
| TAAGCGCGATTATCAGGGAT+AGG | + | 37916:11385-11404 | MS.gene068825:intergenic | 45.0% | |
| TGAAAGGAAGAAAGCGGATG+AGG | - | 37916:11540-11559 | MS.gene068825:CDS | 45.0% | |
| TGTTGTTAGGATGGGAGAGA+GGG | + | 37916:11309-11328 | MS.gene068825:intergenic | 45.0% | |
| TTGTGGAAAAGGGAGAGAGA+GGG | + | 37916:11238-11257 | MS.gene068825:intergenic | 45.0% | |
| ! | AGCTGGAGAGTTTTGCTGAA+AGG | - | 37916:11524-11543 | MS.gene068825:CDS | 45.0% |
| ! | ATAGCGATGATGAGGAGTGT+TGG | + | 37916:11208-11227 | MS.gene068825:intergenic | 45.0% |
| ! | GAAGGATTTGCTTCGAGGTT+TGG | - | 37916:11714-11733 | MS.gene068825:CDS | 45.0% |
| ! | GGTTTGGGTGAGTTTGAGTT+TGG | - | 37916:11730-11749 | MS.gene068825:CDS | 45.0% |
| !! | ATGAGGAGTGTTGGTTTGAG+AGG | + | 37916:11199-11218 | MS.gene068825:intergenic | 45.0% |
| AGAGAGGAGGTTAGGGTATG+TGG | + | 37916:11271-11290 | MS.gene068824:intergenic | 50.0% | |
| AGAGGAGGTTAGGGTATGTG+GGG | + | 37916:11269-11288 | MS.gene068824:intergenic | 50.0% | |
| AGAGGCTGTTGTTGTGTGTG+AGG | + | 37916:11181-11200 | MS.gene068824:intergenic | 50.0% | |
| AGCAGACCACGGTGATTCAT+CGG | + | 37916:11478-11497 | MS.gene068824:intergenic | 50.0% | |
| CATCAACAACGCTTCCAGAC+GGG | + | 37916:11594-11613 | MS.gene068825:intergenic | 50.0% | |
| CCATCAACAACGCTTCCAGA+CGG | + | 37916:11595-11614 | MS.gene068825:intergenic | 50.0% | |
| GAGAGGAGGTTAGGGTATGT+GGG | + | 37916:11270-11289 | MS.gene068825:intergenic | 50.0% | |
| GAGGATGAGAGAGGAGGTTA+GGG | + | 37916:11278-11297 | MS.gene068825:intergenic | 50.0% | |
| GAGGCTGTTGTTGTGTGTGA+GGG | + | 37916:11180-11199 | MS.gene068825:intergenic | 50.0% | |
| GGTGAGGAGGTTGTGGAAAA+GGG | + | 37916:11248-11267 | MS.gene068825:intergenic | 50.0% | |
| GTTGTGGAAAAGGGAGAGAG+AGG | + | 37916:11239-11258 | MS.gene068825:intergenic | 50.0% | |
| ! | GAAGGGAGTGATGGAGAAGA+TGG | + | 37916:11142-11161 | MS.gene068825:intergenic | 50.0% |
| ! | GGATTGGAGTATTACCCGTC+TGG | - | 37916:11577-11596 | MS.gene068825:CDS | 50.0% |
| ! | GTGTTGTTAGGATGGGAGAG+AGG | + | 37916:11310-11329 | MS.gene068825:intergenic | 50.0% |
| !! | GGAAGCGTTGTTGATGGACT+CGG | - | 37916:11598-11617 | MS.gene068825:CDS | 50.0% |
| !! | GGGCTAGCTTGTGTGTTTCT+TGG | - | 37916:11659-11678 | MS.gene068825:CDS | 50.0% |
| !!! | AGGGATAGGGTTTTGAACGG+CGG | + | 37916:11371-11390 | MS.gene068825:intergenic | 50.0% |
| ACGGCGTAAGCGCGATTATC+AGG | + | 37916:11391-11410 | MS.gene068824:intergenic | 55.0% | |
| AGGTTAGGGTATGTGGGGTG+AGG | + | 37916:11264-11283 | MS.gene068824:intergenic | 55.0% | |
| ATCCAGCCGATGAATCACCG+TGG | - | 37916:11469-11488 | MS.gene068825:CDS | 55.0% | |
| CAACCAGATCCGCCCTGATT+CGG | - | 37916:11429-11448 | MS.gene068825:CDS | 55.0% | |
| CGGCGTAAGCGCGATTATCA+GGG | + | 37916:11390-11409 | MS.gene068825:intergenic | 55.0% | |
| CTGACACCGTCCAAGAATGG+CGG | + | 37916:11626-11645 | MS.gene068825:intergenic | 55.0% | |
| CTTGAATGCAACCGCCTCGT+CGG | + | 37916:11506-11525 | MS.gene068825:intergenic | 55.0% | |
| GAGTTCAGGGAGGATGAGAG+AGG | + | 37916:11287-11306 | MS.gene068825:intergenic | 55.0% | |
| GATGGGAGAGAGGGAGTTCA+GGG | + | 37916:11300-11319 | MS.gene068825:intergenic | 55.0% | |
| GGAGGATGAGAGAGGAGGTT+AGG | + | 37916:11279-11298 | MS.gene068825:intergenic | 55.0% | |
| GGGTGAGGAGGTTGTGGAAA+AGG | + | 37916:11249-11268 | MS.gene068825:intergenic | 55.0% | |
| TAACCGAATCAGGGCGGATC+TGG | + | 37916:11435-11454 | MS.gene068825:intergenic | 55.0% | |
| TATGTGGGGTGAGGAGGTTG+TGG | + | 37916:11255-11274 | MS.gene068825:intergenic | 55.0% | |
| TTAGGGTATGTGGGGTGAGG+AGG | + | 37916:11261-11280 | MS.gene068825:intergenic | 55.0% | |
| TTCAGGGAGGATGAGAGAGG+AGG | + | 37916:11284-11303 | MS.gene068825:intergenic | 55.0% | |
| !! | CCGTCTGGAAGCGTTGTTGA+TGG | - | 37916:11592-11611 | MS.gene068825:CDS | 55.0% |
| !! | GGATGGTGGAAGAGAGAGAG+TGG | + | 37916:11342-11361 | MS.gene068825:intergenic | 55.0% |
| CGAGGCGGTTGCATTCAAGC+TGG | - | 37916:11507-11526 | MS.gene068825:CDS | 60.0% | |
| CGTCGGAAGACAGCAGACCA+CGG | + | 37916:11489-11508 | MS.gene068825:intergenic | 60.0% | |
| GACCACGGTGATTCATCGGC+TGG | + | 37916:11474-11493 | MS.gene068825:intergenic | 60.0% | |
| GCTGAGGAGGATTTCCGCGA+CGG | + | 37916:11410-11429 | MS.gene068825:intergenic | 60.0% | |
| GGATGGGAGAGAGGGAGTTC+AGG | + | 37916:11301-11320 | MS.gene068825:intergenic | 60.0% | |
| TAATCGCGCTTACGCCGTCG+CGG | - | 37916:11393-11412 | MS.gene068825:CDS | 60.0% | |
| TCTGCTGTCTTCCGACGAGG+CGG | - | 37916:11492-11511 | MS.gene068825:CDS | 60.0% | |
| TGGTCTGCTGTCTTCCGACG+AGG | - | 37916:11489-11508 | MS.gene068825:CDS | 60.0% | |
| !! | TCGGTGCCGCCATTCTTGGA+CGG | - | 37916:11617-11636 | MS.gene068825:CDS | 60.0% |
| !!! | TAGGGTTTTGAACGGCGGCG+AGG | + | 37916:11366-11385 | MS.gene068825:intergenic | 60.0% |
| CAGGGCGGATCTGGTTGCTG+AGG | + | 37916:11426-11445 | MS.gene068825:intergenic | 65.0% | |
| GGCGGATCTGGTTGCTGAGG+AGG | + | 37916:11423-11442 | MS.gene068825:intergenic | 65.0% | |
| GGGAGAGAGGGAGTTCAGGG+AGG | + | 37916:11297-11316 | MS.gene068825:intergenic | 65.0% | |
| TTGAACGGCGGCGAGGAGGA+TGG | + | 37916:11359-11378 | MS.gene068825:intergenic | 65.0% | |
| !! | GGACTCGGTGCCGCCATTCT+TGG | - | 37916:11613-11632 | MS.gene068825:CDS | 65.0% |
| !!! | GGTTTTGAACGGCGGCGAGG+AGG | + | 37916:11363-11382 | MS.gene068825:intergenic | 65.0% |
| AACGGCGGCGAGGAGGATGG+TGG | + | 37916:11356-11375 | MS.gene068824:intergenic | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 37916 | gene | 11070 | 11825 | 11070 | ID=MS.gene068825 |
| 37916 | mRNA | 11070 | 11825 | 11070 | ID=MS.gene068825.t1;Parent=MS.gene068825 |
| 37916 | exon | 11070 | 11825 | 11070 | ID=MS.gene068825.t1.exon1;Parent=MS.gene068825.t1 |
| 37916 | CDS | 11070 | 11825 | 11070 | ID=cds.MS.gene068825.t1;Parent=MS.gene068825.t1 |
| Gene Sequence |
| Protein sequence |
