AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene053959


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene053959.t1 MTR_5g049260 96.047 253 8 1 1 251 1 253 7.28e-175 481
MS.gene053959.t1 MTR_5g049270 96.047 253 8 1 1 251 1 253 7.28e-175 481
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene053959.t1 AT3G25545 48.936 235 105 5 1 222 1 233 5.38e-62 196

Find 82 sgRNAs with CRISPR-Local

Find 94 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGATGGGAGAGAGGGAGTTC+AGG 0.193955 5.3:-40968768 None:intergenic
GGGCTAGCTTGTGTGTTTCT+TGG 0.231999 5.3:+40969129 MS.gene053959:CDS
ACGGCGTAAGCGCGATTATC+AGG 0.233809 5.3:-40968858 None:intergenic
GGACTCGGTGCCGCCATTCT+TGG 0.292638 5.3:+40969083 MS.gene053959:CDS
GGTTTGGGTGAGTTTGAGTT+TGG 0.295588 5.3:+40969200 MS.gene053959:CDS
GGAGGATGAGAGAGGAGGTT+AGG 0.297528 5.3:-40968746 None:intergenic
AAGGATTTGCTTCGAGGTTT+GGG 0.314069 5.3:+40969185 MS.gene053959:CDS
GAGGATGAGAGAGGAGGTTA+GGG 0.324797 5.3:-40968745 None:intergenic
AGAGAGGAGGTTAGGGTATG+TGG 0.351847 5.3:-40968738 None:intergenic
GAAGGATTTGCTTCGAGGTT+TGG 0.357970 5.3:+40969184 MS.gene053959:CDS
TCAAGTATGATAACCGAATC+AGG 0.365883 5.3:-40968912 None:intergenic
GATGGGAGAGAGGGAGTTCA+GGG 0.377552 5.3:-40968767 None:intergenic
GGGTGAGGAGGTTGTGGAAA+AGG 0.377658 5.3:-40968716 None:intergenic
AAGATGGTGTGAAATTGTTT+AGG 0.389664 5.3:-40968593 None:intergenic
TCTTCTTCTAGTAATCTTGA+TGG 0.395897 5.3:-40968570 None:intergenic
GTTTGGGTGAGTTTGAGTTT+GGG 0.402440 5.3:+40969201 MS.gene053959:CDS
CCGTCTGGAAGCGTTGTTGA+TGG 0.413333 5.3:+40969062 MS.gene053959:CDS
AGGTTAGGGTATGTGGGGTG+AGG 0.413432 5.3:-40968731 None:intergenic
GAGAGGAGGTTAGGGTATGT+GGG 0.431391 5.3:-40968737 None:intergenic
TGGATGTTGATGGTGTTGTT+AGG 0.444194 5.3:-40968789 None:intergenic
GTTGTGGAAAAGGGAGAGAG+AGG 0.451397 5.3:-40968706 None:intergenic
TTTCAAATTTGGGAGGAGTA+AGG 0.452557 5.3:+40969241 MS.gene053959:CDS
TAACCGAATCAGGGCGGATC+TGG 0.453361 5.3:-40968902 None:intergenic
GGTGAGGAGGTTGTGGAAAA+GGG 0.458126 5.3:-40968715 None:intergenic
GGAGAAATTGTTGAGTGGAT+TGG 0.462542 5.3:+40969031 MS.gene053959:CDS
TGTTGATGGTGTTGTTAGGA+TGG 0.464009 5.3:-40968785 None:intergenic
GGATGGTGGAAGAGAGAGAG+TGG 0.466243 5.3:-40968809 None:intergenic
CAACCAGATCCGCCCTGATT+CGG 0.467578 5.3:+40968899 MS.gene053959:CDS
TTAGGGTATGTGGGGTGAGG+AGG 0.469748 5.3:-40968728 None:intergenic
TATGTGGGGTGAGGAGGTTG+TGG 0.473676 5.3:-40968722 None:intergenic
AGAAAAGGTGAAGGGAGTGA+TGG 0.475108 5.3:-40968618 None:intergenic
TTGTTGAAGGATTTGCTTCG+AGG 0.480507 5.3:+40969179 MS.gene053959:CDS
AGAGAGAGAGTGGATGTTGA+TGG 0.485843 5.3:-40968799 None:intergenic
TTGAACGGCGGCGAGGAGGA+TGG 0.486410 5.3:-40968826 None:intergenic
GTGTTGTTAGGATGGGAGAG+AGG 0.495674 5.3:-40968777 None:intergenic
GACCACGGTGATTCATCGGC+TGG 0.499631 5.3:-40968941 None:intergenic
GGATTGGAGTATTACCCGTC+TGG 0.503439 5.3:+40969047 MS.gene053959:CDS
AATCTGACACCGTCCAAGAA+TGG 0.503968 5.3:-40969096 None:intergenic
GAGTTCAGGGAGGATGAGAG+AGG 0.511477 5.3:-40968754 None:intergenic
GGCGGATCTGGTTGCTGAGG+AGG 0.512243 5.3:-40968890 None:intergenic
ATAGCGATGATGAGGAGTGT+TGG 0.523299 5.3:-40968675 None:intergenic
GAAGGGAGTGATGGAGAAGA+TGG 0.529330 5.3:-40968609 None:intergenic
AGAGGCTGTTGTTGTGTGTG+AGG 0.529595 5.3:-40968648 None:intergenic
TTGTGGAAAAGGGAGAGAGA+GGG 0.534591 5.3:-40968705 None:intergenic
ATGAGGAGTGTTGGTTTGAG+AGG 0.539310 5.3:-40968666 None:intergenic
AGCAGACCACGGTGATTCAT+CGG 0.542746 5.3:-40968945 None:intergenic
GAGAAGAGATAGCGATGATG+AGG 0.544694 5.3:-40968683 None:intergenic
TGTTGTTAGGATGGGAGAGA+GGG 0.545857 5.3:-40968776 None:intergenic
GTTGTTGTGTGTGAGGGAAA+AGG 0.547021 5.3:-40968641 None:intergenic
TGAAAGGAAGAAAGCGGATG+AGG 0.548854 5.3:+40969010 MS.gene053959:CDS
GGGAAAAGGAGAAAAGGTGA+AGG 0.553523 5.3:-40968627 None:intergenic
ACAACATCTGAATCAAATTG+AGG 0.556465 5.3:-40969152 None:intergenic
GGGAGAGAGGGAGTTCAGGG+AGG 0.560544 5.3:-40968764 None:intergenic
GGAAGCGTTGTTGATGGACT+CGG 0.567830 5.3:+40969068 MS.gene053959:CDS
GAGGCTGTTGTTGTGTGTGA+GGG 0.570892 5.3:-40968647 None:intergenic
GTTGATGGTGTTGTTAGGAT+GGG 0.571485 5.3:-40968784 None:intergenic
AAGCGCGATTATCAGGGATA+GGG 0.574208 5.3:-40968851 None:intergenic
CATCAACAACGCTTCCAGAC+GGG 0.576803 5.3:-40969061 None:intergenic
GTTTGGGTTTAGTGATGATG+AGG 0.577632 5.3:+40969217 MS.gene053959:CDS
TAAGCGCGATTATCAGGGAT+AGG 0.580517 5.3:-40968852 None:intergenic
GCTGAGGAGGATTTCCGCGA+CGG 0.583968 5.3:-40968877 None:intergenic
CGAGGCGGTTGCATTCAAGC+TGG 0.584066 5.3:+40968977 MS.gene053959:CDS
CTTGAATGCAACCGCCTCGT+CGG 0.585956 5.3:-40968973 None:intergenic
GGAAAAGGAGAAAAGGTGAA+GGG 0.589592 5.3:-40968626 None:intergenic
CGGCGTAAGCGCGATTATCA+GGG 0.590741 5.3:-40968857 None:intergenic
TTCAGGGAGGATGAGAGAGG+AGG 0.592701 5.3:-40968751 None:intergenic
TCTTCTAGTAATCTTGATGG+TGG 0.594403 5.3:-40968567 None:intergenic
AACGGCGGCGAGGAGGATGG+TGG 0.595481 5.3:-40968823 None:intergenic
AGAGGAGGTTAGGGTATGTG+GGG 0.597052 5.3:-40968736 None:intergenic
GTGTGAGGGAAAAGGAGAAA+AGG 0.601573 5.3:-40968633 None:intergenic
TCGGTGCCGCCATTCTTGGA+CGG 0.607388 5.3:+40969087 MS.gene053959:CDS
GATGAGGAGAAATTGTTGAG+TGG 0.611429 5.3:+40969026 MS.gene053959:CDS
CCATCAACAACGCTTCCAGA+CGG 0.615004 5.3:-40969062 None:intergenic
CAGGGCGGATCTGGTTGCTG+AGG 0.621735 5.3:-40968893 None:intergenic
CAAGTATGATAACCGAATCA+GGG 0.627258 5.3:-40968911 None:intergenic
TGGTCTGCTGTCTTCCGACG+AGG 0.661086 5.3:+40968959 MS.gene053959:CDS
TCTGCTGTCTTCCGACGAGG+CGG 0.672935 5.3:+40968962 MS.gene053959:CDS
CGTCGGAAGACAGCAGACCA+CGG 0.686393 5.3:-40968956 None:intergenic
TAATCGCGCTTACGCCGTCG+CGG 0.704779 5.3:+40968863 MS.gene053959:CDS
CTGACACCGTCCAAGAATGG+CGG 0.706584 5.3:-40969093 None:intergenic
GTATGATAACCGAATCAGGG+CGG 0.767199 5.3:-40968908 None:intergenic
ATCCAGCCGATGAATCACCG+TGG 0.786438 5.3:+40968939 MS.gene053959:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATGATGAGGTTTTCAAATTT+GGG + chr5.3:40969231-40969250 MS.gene053959:CDS 25.0%
!!! GTGTCAGATTTTGAAAATTA+GGG + chr5.3:40969109-40969128 MS.gene053959:CDS 25.0%
AAGATGGTGTGAAATTGTTT+AGG - chr5.3:40968596-40968615 None:intergenic 30.0%
ACAACATCTGAATCAAATTG+AGG - chr5.3:40969155-40969174 None:intergenic 30.0%
TCTTCTTCTAGTAATCTTGA+TGG - chr5.3:40968573-40968592 None:intergenic 30.0%
! GATGATGAGGTTTTCAAATT+TGG + chr5.3:40969230-40969249 MS.gene053959:CDS 30.0%
!! GGTGTCAGATTTTGAAAATT+AGG + chr5.3:40969108-40969127 MS.gene053959:CDS 30.0%
!!! AGATGTTGTTTTGTTGTTGA+AGG + chr5.3:40969166-40969185 MS.gene053959:CDS 30.0%
CAAGTATGATAACCGAATCA+GGG - chr5.3:40968914-40968933 None:intergenic 35.0%
TCAAGTATGATAACCGAATC+AGG - chr5.3:40968915-40968934 None:intergenic 35.0%
TCTTCTAGTAATCTTGATGG+TGG - chr5.3:40968570-40968589 None:intergenic 35.0%
TTTCAAATTTGGGAGGAGTA+AGG + chr5.3:40969241-40969260 MS.gene053959:CDS 35.0%
! ATGAGGTTTTCAAATTTGGG+AGG + chr5.3:40969234-40969253 MS.gene053959:CDS 35.0%
! TTTTGCTGAAAGGAAGAAAG+CGG + chr5.3:40969004-40969023 MS.gene053959:CDS 35.0%
GATGAGGAGAAATTGTTGAG+TGG + chr5.3:40969026-40969045 MS.gene053959:CDS 40.0%
GGAAAAGGAGAAAAGGTGAA+GGG - chr5.3:40968629-40968648 None:intergenic 40.0%
GGAGAAATTGTTGAGTGGAT+TGG + chr5.3:40969031-40969050 MS.gene053959:CDS 40.0%
! AAGGATTTGCTTCGAGGTTT+GGG + chr5.3:40969185-40969204 MS.gene053959:CDS 40.0%
! GTTTGGGTGAGTTTGAGTTT+GGG + chr5.3:40969201-40969220 MS.gene053959:CDS 40.0%
! GTTTGGGTTTAGTGATGATG+AGG + chr5.3:40969217-40969236 MS.gene053959:CDS 40.0%
! TTGTTGAAGGATTTGCTTCG+AGG + chr5.3:40969179-40969198 MS.gene053959:CDS 40.0%
!! GTTGATGGTGTTGTTAGGAT+GGG - chr5.3:40968787-40968806 None:intergenic 40.0%
!! TGGATGTTGATGGTGTTGTT+AGG - chr5.3:40968792-40968811 None:intergenic 40.0%
!! TGTTGATGGTGTTGTTAGGA+TGG - chr5.3:40968788-40968807 None:intergenic 40.0%
!!! ATCAGGGATAGGGTTTTGAA+CGG - chr5.3:40968844-40968863 None:intergenic 40.0%
AAGCGCGATTATCAGGGATA+GGG - chr5.3:40968854-40968873 None:intergenic 45.0%
AATCTGACACCGTCCAAGAA+TGG - chr5.3:40969099-40969118 None:intergenic 45.0%
AGAAAAGGTGAAGGGAGTGA+TGG - chr5.3:40968621-40968640 None:intergenic 45.0%
AGAGAGAGAGTGGATGTTGA+TGG - chr5.3:40968802-40968821 None:intergenic 45.0%
GAGAAGAGATAGCGATGATG+AGG - chr5.3:40968686-40968705 None:intergenic 45.0%
GGGAAAAGGAGAAAAGGTGA+AGG - chr5.3:40968630-40968649 None:intergenic 45.0%
GTATGATAACCGAATCAGGG+CGG - chr5.3:40968911-40968930 None:intergenic 45.0%
GTGTGAGGGAAAAGGAGAAA+AGG - chr5.3:40968636-40968655 None:intergenic 45.0%
GTTGTTGTGTGTGAGGGAAA+AGG - chr5.3:40968644-40968663 None:intergenic 45.0%
TAAGCGCGATTATCAGGGAT+AGG - chr5.3:40968855-40968874 None:intergenic 45.0%
TGAAAGGAAGAAAGCGGATG+AGG + chr5.3:40969010-40969029 MS.gene053959:CDS 45.0%
TGTTGTTAGGATGGGAGAGA+GGG - chr5.3:40968779-40968798 None:intergenic 45.0%
TTGTGGAAAAGGGAGAGAGA+GGG - chr5.3:40968708-40968727 None:intergenic 45.0%
! AGCTGGAGAGTTTTGCTGAA+AGG + chr5.3:40968994-40969013 MS.gene053959:CDS 45.0%
! ATAGCGATGATGAGGAGTGT+TGG - chr5.3:40968678-40968697 None:intergenic 45.0%
! GAAGGATTTGCTTCGAGGTT+TGG + chr5.3:40969184-40969203 MS.gene053959:CDS 45.0%
! GGTTTGGGTGAGTTTGAGTT+TGG + chr5.3:40969200-40969219 MS.gene053959:CDS 45.0%
!! ATGAGGAGTGTTGGTTTGAG+AGG - chr5.3:40968669-40968688 None:intergenic 45.0%
AGAGAGGAGGTTAGGGTATG+TGG - chr5.3:40968741-40968760 None:intergenic 50.0%
AGAGGAGGTTAGGGTATGTG+GGG - chr5.3:40968739-40968758 None:intergenic 50.0%
AGAGGCTGTTGTTGTGTGTG+AGG - chr5.3:40968651-40968670 None:intergenic 50.0%
AGCAGACCACGGTGATTCAT+CGG - chr5.3:40968948-40968967 None:intergenic 50.0%
CATCAACAACGCTTCCAGAC+GGG - chr5.3:40969064-40969083 None:intergenic 50.0%
CCATCAACAACGCTTCCAGA+CGG - chr5.3:40969065-40969084 None:intergenic 50.0%
GAGAGGAGGTTAGGGTATGT+GGG - chr5.3:40968740-40968759 None:intergenic 50.0%
GAGGATGAGAGAGGAGGTTA+GGG - chr5.3:40968748-40968767 None:intergenic 50.0%
GAGGCTGTTGTTGTGTGTGA+GGG - chr5.3:40968650-40968669 None:intergenic 50.0%
GGTGAGGAGGTTGTGGAAAA+GGG - chr5.3:40968718-40968737 None:intergenic 50.0%
GTTGTGGAAAAGGGAGAGAG+AGG - chr5.3:40968709-40968728 None:intergenic 50.0%
! GAAGGGAGTGATGGAGAAGA+TGG - chr5.3:40968612-40968631 None:intergenic 50.0%
! GGATTGGAGTATTACCCGTC+TGG + chr5.3:40969047-40969066 MS.gene053959:CDS 50.0%
! GTGTTGTTAGGATGGGAGAG+AGG - chr5.3:40968780-40968799 None:intergenic 50.0%
!! GGAAGCGTTGTTGATGGACT+CGG + chr5.3:40969068-40969087 MS.gene053959:CDS 50.0%
!! GGGCTAGCTTGTGTGTTTCT+TGG + chr5.3:40969129-40969148 MS.gene053959:CDS 50.0%
!!! AGGGATAGGGTTTTGAACGG+CGG - chr5.3:40968841-40968860 None:intergenic 50.0%
ACGGCGTAAGCGCGATTATC+AGG - chr5.3:40968861-40968880 None:intergenic 55.0%
AGGTTAGGGTATGTGGGGTG+AGG - chr5.3:40968734-40968753 None:intergenic 55.0%
ATCCAGCCGATGAATCACCG+TGG + chr5.3:40968939-40968958 MS.gene053959:CDS 55.0%
CAACCAGATCCGCCCTGATT+CGG + chr5.3:40968899-40968918 MS.gene053959:CDS 55.0%
CGGCGTAAGCGCGATTATCA+GGG - chr5.3:40968860-40968879 None:intergenic 55.0%
CTGACACCGTCCAAGAATGG+CGG - chr5.3:40969096-40969115 None:intergenic 55.0%
CTTGAATGCAACCGCCTCGT+CGG - chr5.3:40968976-40968995 None:intergenic 55.0%
GAGTTCAGGGAGGATGAGAG+AGG - chr5.3:40968757-40968776 None:intergenic 55.0%
GATGGGAGAGAGGGAGTTCA+GGG - chr5.3:40968770-40968789 None:intergenic 55.0%
GGAGGATGAGAGAGGAGGTT+AGG - chr5.3:40968749-40968768 None:intergenic 55.0%
GGGTGAGGAGGTTGTGGAAA+AGG - chr5.3:40968719-40968738 None:intergenic 55.0%
TAACCGAATCAGGGCGGATC+TGG - chr5.3:40968905-40968924 None:intergenic 55.0%
TATGTGGGGTGAGGAGGTTG+TGG - chr5.3:40968725-40968744 None:intergenic 55.0%
TTAGGGTATGTGGGGTGAGG+AGG - chr5.3:40968731-40968750 None:intergenic 55.0%
TTCAGGGAGGATGAGAGAGG+AGG - chr5.3:40968754-40968773 None:intergenic 55.0%
!! CCGTCTGGAAGCGTTGTTGA+TGG + chr5.3:40969062-40969081 MS.gene053959:CDS 55.0%
!! GGATGGTGGAAGAGAGAGAG+TGG - chr5.3:40968812-40968831 None:intergenic 55.0%
CGAGGCGGTTGCATTCAAGC+TGG + chr5.3:40968977-40968996 MS.gene053959:CDS 60.0%
CGTCGGAAGACAGCAGACCA+CGG - chr5.3:40968959-40968978 None:intergenic 60.0%
GACCACGGTGATTCATCGGC+TGG - chr5.3:40968944-40968963 None:intergenic 60.0%
GCTGAGGAGGATTTCCGCGA+CGG - chr5.3:40968880-40968899 None:intergenic 60.0%
GGATGGGAGAGAGGGAGTTC+AGG - chr5.3:40968771-40968790 None:intergenic 60.0%
TAATCGCGCTTACGCCGTCG+CGG + chr5.3:40968863-40968882 MS.gene053959:CDS 60.0%
TCTGCTGTCTTCCGACGAGG+CGG + chr5.3:40968962-40968981 MS.gene053959:CDS 60.0%
TGGTCTGCTGTCTTCCGACG+AGG + chr5.3:40968959-40968978 MS.gene053959:CDS 60.0%
!! TCGGTGCCGCCATTCTTGGA+CGG + chr5.3:40969087-40969106 MS.gene053959:CDS 60.0%
!!! TAGGGTTTTGAACGGCGGCG+AGG - chr5.3:40968836-40968855 None:intergenic 60.0%
CAGGGCGGATCTGGTTGCTG+AGG - chr5.3:40968896-40968915 None:intergenic 65.0%
GGCGGATCTGGTTGCTGAGG+AGG - chr5.3:40968893-40968912 None:intergenic 65.0%
GGGAGAGAGGGAGTTCAGGG+AGG - chr5.3:40968767-40968786 None:intergenic 65.0%
TTGAACGGCGGCGAGGAGGA+TGG - chr5.3:40968829-40968848 None:intergenic 65.0%
!! GGACTCGGTGCCGCCATTCT+TGG + chr5.3:40969083-40969102 MS.gene053959:CDS 65.0%
!!! GGTTTTGAACGGCGGCGAGG+AGG - chr5.3:40968833-40968852 None:intergenic 65.0%
AACGGCGGCGAGGAGGATGG+TGG - chr5.3:40968826-40968845 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr5.3 gene 40968540 40969295 40968540 ID=MS.gene053959
chr5.3 mRNA 40968540 40969295 40968540 ID=MS.gene053959.t1;Parent=MS.gene053959
chr5.3 exon 40968540 40969295 40968540 ID=MS.gene053959.t1.exon1;Parent=MS.gene053959.t1
chr5.3 CDS 40968540 40969295 40968540 ID=cds.MS.gene053959.t1;Parent=MS.gene053959.t1
Gene Sequence

>MS.gene053959

ATGGAAGATGTGATAACAGAAGCAGCACCACCATCAAGATTACTAGAAGAAGACCTAAACAATTTCACACCATCTTCTCCATCACTCCCTTCACCTTTTCTCCTTTTCCCTCACACACAACAACAGCCTCTCAAACCAACACTCCTCATCATCGCTATCTCTTCTCCCTCTCTCTCCCTTTTCCACAACCTCCTCACCCCACATACCCTAACCTCCTCTCTCATCCTCCCTGAACTCCCTCTCTCCCATCCTAACAACACCATCAACATCCACTCTCTCTCTTCCACCATCCTCCTCGCCGCCGTTCAAAACCCTATCCCTGATAATCGCGCTTACGCCGTCGCGGAAATCCTCCTCAGCAACCAGATCCGCCCTGATTCGGTTATCATACTTGATTCGATCCAGCCGATGAATCACCGTGGTCTGCTGTCTTCCGACGAGGCGGTTGCATTCAAGCTGGAGAGTTTTGCTGAAAGGAAGAAAGCGGATGAGGAGAAATTGTTGAGTGGATTGGAGTATTACCCGTCTGGAAGCGTTGTTGATGGACTCGGTGCCGCCATTCTTGGACGGTGTCAGATTTTGAAAATTAGGGCTAGCTTGTGTGTTTCTTGGCCTCAATTTGATTCAGATGTTGTTTTGTTGTTGAAGGATTTGCTTCGAGGTTTGGGTGAGTTTGAGTTTGGGTTTAGTGATGATGAGGTTTTCAAATTTGGGAGGAGTAAGGATCATGTTTTTCAATCTCACTTGTATATTTGA

Protein sequence

>MS.gene053959.t1

MEDVITEAAPPSRLLEEDLNNFTPSSPSLPSPFLLFPHTQQQPLKPTLLIIAISSPSLSLFHNLLTPHTLTSSLILPELPLSHPNNTINIHSLSSTILLAAVQNPIPDNRAYAVAEILLSNQIRPDSVIILDSIQPMNHRGLLSSDEAVAFKLESFAERKKADEEKLLSGLEYYPSGSVVDGLGAAILGRCQILKIRASLCVSWPQFDSDVVLLLKDLLRGLGEFEFGFSDDEVFKFGRSKDHVFQSHLYI