AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene068827


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene068827.t1 MTR_5g049260 96.047 253 8 1 1 251 1 253 7.28e-175 481
MS.gene068827.t1 MTR_5g049270 96.047 253 8 1 1 251 1 253 7.28e-175 481
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene068827.t1 AT3G25545 48.936 235 105 5 1 222 1 233 5.38e-62 196

Find 82 sgRNAs with CRISPR-Local

Find 94 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGATGGGAGAGAGGGAGTTC+AGG 0.193955 5.2:-43167129 None:intergenic
GGGCTAGCTTGTGTGTTTCT+TGG 0.231999 5.2:+43167490 MS.gene068827:CDS
ACGGCGTAAGCGCGATTATC+AGG 0.233809 5.2:-43167219 None:intergenic
GGACTCGGTGCCGCCATTCT+TGG 0.292638 5.2:+43167444 MS.gene068827:CDS
GGTTTGGGTGAGTTTGAGTT+TGG 0.295588 5.2:+43167561 MS.gene068827:CDS
GGAGGATGAGAGAGGAGGTT+AGG 0.297528 5.2:-43167107 None:intergenic
AAGGATTTGCTTCGAGGTTT+GGG 0.314069 5.2:+43167546 MS.gene068827:CDS
GAGGATGAGAGAGGAGGTTA+GGG 0.324797 5.2:-43167106 None:intergenic
AGAGAGGAGGTTAGGGTATG+TGG 0.351847 5.2:-43167099 None:intergenic
GAAGGATTTGCTTCGAGGTT+TGG 0.357970 5.2:+43167545 MS.gene068827:CDS
TCAAGTATGATAACCGAATC+AGG 0.365883 5.2:-43167273 None:intergenic
GATGGGAGAGAGGGAGTTCA+GGG 0.377552 5.2:-43167128 None:intergenic
GGGTGAGGAGGTTGTGGAAA+AGG 0.377658 5.2:-43167077 None:intergenic
AAGATGGTGTGAAATTGTTT+AGG 0.389664 5.2:-43166954 None:intergenic
TCTTCTTCTAGTAATCTTGA+TGG 0.395897 5.2:-43166931 None:intergenic
GTTTGGGTGAGTTTGAGTTT+GGG 0.402440 5.2:+43167562 MS.gene068827:CDS
CCGTCTGGAAGCGTTGTTGA+TGG 0.413333 5.2:+43167423 MS.gene068827:CDS
AGGTTAGGGTATGTGGGGTG+AGG 0.413432 5.2:-43167092 None:intergenic
GAGAGGAGGTTAGGGTATGT+GGG 0.431391 5.2:-43167098 None:intergenic
TGGATGTTGATGGTGTTGTT+AGG 0.444194 5.2:-43167150 None:intergenic
GTTGTGGAAAAGGGAGAGAG+AGG 0.451397 5.2:-43167067 None:intergenic
TTTCAAATTTGGGAGGAGTA+AGG 0.452557 5.2:+43167602 MS.gene068827:CDS
TAACCGAATCAGGGCGGATC+TGG 0.453361 5.2:-43167263 None:intergenic
GGTGAGGAGGTTGTGGAAAA+GGG 0.458126 5.2:-43167076 None:intergenic
GGAGAAATTGTTGAGTGGAT+TGG 0.462542 5.2:+43167392 MS.gene068827:CDS
TGTTGATGGTGTTGTTAGGA+TGG 0.464009 5.2:-43167146 None:intergenic
GGATGGTGGAAGAGAGAGAG+TGG 0.466243 5.2:-43167170 None:intergenic
CAACCAGATCCGCCCTGATT+CGG 0.467578 5.2:+43167260 MS.gene068827:CDS
TTAGGGTATGTGGGGTGAGG+AGG 0.469748 5.2:-43167089 None:intergenic
TATGTGGGGTGAGGAGGTTG+TGG 0.473676 5.2:-43167083 None:intergenic
AGAAAAGGTGAAGGGAGTGA+TGG 0.475108 5.2:-43166979 None:intergenic
TTGTTGAAGGATTTGCTTCG+AGG 0.480507 5.2:+43167540 MS.gene068827:CDS
AGAGAGAGAGTGGATGTTGA+TGG 0.485843 5.2:-43167160 None:intergenic
TTGAACGGCGGCGAGGAGGA+TGG 0.486410 5.2:-43167187 None:intergenic
GTGTTGTTAGGATGGGAGAG+AGG 0.495674 5.2:-43167138 None:intergenic
GACCACGGTGATTCATCGGC+TGG 0.499631 5.2:-43167302 None:intergenic
GGATTGGAGTATTACCCGTC+TGG 0.503439 5.2:+43167408 MS.gene068827:CDS
AATCTGACACCGTCCAAGAA+TGG 0.503968 5.2:-43167457 None:intergenic
GAGTTCAGGGAGGATGAGAG+AGG 0.511477 5.2:-43167115 None:intergenic
GGCGGATCTGGTTGCTGAGG+AGG 0.512243 5.2:-43167251 None:intergenic
ATAGCGATGATGAGGAGTGT+TGG 0.523299 5.2:-43167036 None:intergenic
GAAGGGAGTGATGGAGAAGA+TGG 0.529330 5.2:-43166970 None:intergenic
AGAGGCTGTTGTTGTGTGTG+AGG 0.529595 5.2:-43167009 None:intergenic
TTGTGGAAAAGGGAGAGAGA+GGG 0.534591 5.2:-43167066 None:intergenic
ATGAGGAGTGTTGGTTTGAG+AGG 0.539310 5.2:-43167027 None:intergenic
AGCAGACCACGGTGATTCAT+CGG 0.542746 5.2:-43167306 None:intergenic
GAGAAGAGATAGCGATGATG+AGG 0.544694 5.2:-43167044 None:intergenic
TGTTGTTAGGATGGGAGAGA+GGG 0.545857 5.2:-43167137 None:intergenic
GTTGTTGTGTGTGAGGGAAA+AGG 0.547021 5.2:-43167002 None:intergenic
TGAAAGGAAGAAAGCGGATG+AGG 0.548854 5.2:+43167371 MS.gene068827:CDS
GGGAAAAGGAGAAAAGGTGA+AGG 0.553523 5.2:-43166988 None:intergenic
ACAACATCTGAATCAAATTG+AGG 0.556465 5.2:-43167513 None:intergenic
GGGAGAGAGGGAGTTCAGGG+AGG 0.560544 5.2:-43167125 None:intergenic
GGAAGCGTTGTTGATGGACT+CGG 0.567830 5.2:+43167429 MS.gene068827:CDS
GAGGCTGTTGTTGTGTGTGA+GGG 0.570892 5.2:-43167008 None:intergenic
GTTGATGGTGTTGTTAGGAT+GGG 0.571485 5.2:-43167145 None:intergenic
AAGCGCGATTATCAGGGATA+GGG 0.574208 5.2:-43167212 None:intergenic
CATCAACAACGCTTCCAGAC+GGG 0.576803 5.2:-43167422 None:intergenic
GTTTGGGTTTAGTGATGATG+AGG 0.577632 5.2:+43167578 MS.gene068827:CDS
TAAGCGCGATTATCAGGGAT+AGG 0.580517 5.2:-43167213 None:intergenic
GCTGAGGAGGATTTCCGCGA+CGG 0.583968 5.2:-43167238 None:intergenic
CGAGGCGGTTGCATTCAAGC+TGG 0.584066 5.2:+43167338 MS.gene068827:CDS
CTTGAATGCAACCGCCTCGT+CGG 0.585956 5.2:-43167334 None:intergenic
GGAAAAGGAGAAAAGGTGAA+GGG 0.589592 5.2:-43166987 None:intergenic
CGGCGTAAGCGCGATTATCA+GGG 0.590741 5.2:-43167218 None:intergenic
TTCAGGGAGGATGAGAGAGG+AGG 0.592701 5.2:-43167112 None:intergenic
TCTTCTAGTAATCTTGATGG+TGG 0.594403 5.2:-43166928 None:intergenic
AACGGCGGCGAGGAGGATGG+TGG 0.595481 5.2:-43167184 None:intergenic
AGAGGAGGTTAGGGTATGTG+GGG 0.597052 5.2:-43167097 None:intergenic
GTGTGAGGGAAAAGGAGAAA+AGG 0.601573 5.2:-43166994 None:intergenic
TCGGTGCCGCCATTCTTGGA+CGG 0.607388 5.2:+43167448 MS.gene068827:CDS
GATGAGGAGAAATTGTTGAG+TGG 0.611429 5.2:+43167387 MS.gene068827:CDS
CCATCAACAACGCTTCCAGA+CGG 0.615004 5.2:-43167423 None:intergenic
CAGGGCGGATCTGGTTGCTG+AGG 0.621735 5.2:-43167254 None:intergenic
CAAGTATGATAACCGAATCA+GGG 0.627258 5.2:-43167272 None:intergenic
TGGTCTGCTGTCTTCCGACG+AGG 0.661086 5.2:+43167320 MS.gene068827:CDS
TCTGCTGTCTTCCGACGAGG+CGG 0.672935 5.2:+43167323 MS.gene068827:CDS
CGTCGGAAGACAGCAGACCA+CGG 0.686393 5.2:-43167317 None:intergenic
TAATCGCGCTTACGCCGTCG+CGG 0.704779 5.2:+43167224 MS.gene068827:CDS
CTGACACCGTCCAAGAATGG+CGG 0.706584 5.2:-43167454 None:intergenic
GTATGATAACCGAATCAGGG+CGG 0.767199 5.2:-43167269 None:intergenic
ATCCAGCCGATGAATCACCG+TGG 0.786438 5.2:+43167300 MS.gene068827:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATGATGAGGTTTTCAAATTT+GGG + chr5.2:43167592-43167611 MS.gene068827:CDS 25.0%
!!! GTGTCAGATTTTGAAAATTA+GGG + chr5.2:43167470-43167489 MS.gene068827:CDS 25.0%
AAGATGGTGTGAAATTGTTT+AGG - chr5.2:43166957-43166976 None:intergenic 30.0%
ACAACATCTGAATCAAATTG+AGG - chr5.2:43167516-43167535 None:intergenic 30.0%
TCTTCTTCTAGTAATCTTGA+TGG - chr5.2:43166934-43166953 None:intergenic 30.0%
! GATGATGAGGTTTTCAAATT+TGG + chr5.2:43167591-43167610 MS.gene068827:CDS 30.0%
!! GGTGTCAGATTTTGAAAATT+AGG + chr5.2:43167469-43167488 MS.gene068827:CDS 30.0%
!!! AGATGTTGTTTTGTTGTTGA+AGG + chr5.2:43167527-43167546 MS.gene068827:CDS 30.0%
CAAGTATGATAACCGAATCA+GGG - chr5.2:43167275-43167294 None:intergenic 35.0%
TCAAGTATGATAACCGAATC+AGG - chr5.2:43167276-43167295 None:intergenic 35.0%
TCTTCTAGTAATCTTGATGG+TGG - chr5.2:43166931-43166950 None:intergenic 35.0%
TTTCAAATTTGGGAGGAGTA+AGG + chr5.2:43167602-43167621 MS.gene068827:CDS 35.0%
! ATGAGGTTTTCAAATTTGGG+AGG + chr5.2:43167595-43167614 MS.gene068827:CDS 35.0%
! TTTTGCTGAAAGGAAGAAAG+CGG + chr5.2:43167365-43167384 MS.gene068827:CDS 35.0%
GATGAGGAGAAATTGTTGAG+TGG + chr5.2:43167387-43167406 MS.gene068827:CDS 40.0%
GGAAAAGGAGAAAAGGTGAA+GGG - chr5.2:43166990-43167009 None:intergenic 40.0%
GGAGAAATTGTTGAGTGGAT+TGG + chr5.2:43167392-43167411 MS.gene068827:CDS 40.0%
! AAGGATTTGCTTCGAGGTTT+GGG + chr5.2:43167546-43167565 MS.gene068827:CDS 40.0%
! GTTTGGGTGAGTTTGAGTTT+GGG + chr5.2:43167562-43167581 MS.gene068827:CDS 40.0%
! GTTTGGGTTTAGTGATGATG+AGG + chr5.2:43167578-43167597 MS.gene068827:CDS 40.0%
! TTGTTGAAGGATTTGCTTCG+AGG + chr5.2:43167540-43167559 MS.gene068827:CDS 40.0%
!! GTTGATGGTGTTGTTAGGAT+GGG - chr5.2:43167148-43167167 None:intergenic 40.0%
!! TGGATGTTGATGGTGTTGTT+AGG - chr5.2:43167153-43167172 None:intergenic 40.0%
!! TGTTGATGGTGTTGTTAGGA+TGG - chr5.2:43167149-43167168 None:intergenic 40.0%
!!! ATCAGGGATAGGGTTTTGAA+CGG - chr5.2:43167205-43167224 None:intergenic 40.0%
AAGCGCGATTATCAGGGATA+GGG - chr5.2:43167215-43167234 None:intergenic 45.0%
AATCTGACACCGTCCAAGAA+TGG - chr5.2:43167460-43167479 None:intergenic 45.0%
AGAAAAGGTGAAGGGAGTGA+TGG - chr5.2:43166982-43167001 None:intergenic 45.0%
AGAGAGAGAGTGGATGTTGA+TGG - chr5.2:43167163-43167182 None:intergenic 45.0%
GAGAAGAGATAGCGATGATG+AGG - chr5.2:43167047-43167066 None:intergenic 45.0%
GGGAAAAGGAGAAAAGGTGA+AGG - chr5.2:43166991-43167010 None:intergenic 45.0%
GTATGATAACCGAATCAGGG+CGG - chr5.2:43167272-43167291 None:intergenic 45.0%
GTGTGAGGGAAAAGGAGAAA+AGG - chr5.2:43166997-43167016 None:intergenic 45.0%
GTTGTTGTGTGTGAGGGAAA+AGG - chr5.2:43167005-43167024 None:intergenic 45.0%
TAAGCGCGATTATCAGGGAT+AGG - chr5.2:43167216-43167235 None:intergenic 45.0%
TGAAAGGAAGAAAGCGGATG+AGG + chr5.2:43167371-43167390 MS.gene068827:CDS 45.0%
TGTTGTTAGGATGGGAGAGA+GGG - chr5.2:43167140-43167159 None:intergenic 45.0%
TTGTGGAAAAGGGAGAGAGA+GGG - chr5.2:43167069-43167088 None:intergenic 45.0%
! AGCTGGAGAGTTTTGCTGAA+AGG + chr5.2:43167355-43167374 MS.gene068827:CDS 45.0%
! ATAGCGATGATGAGGAGTGT+TGG - chr5.2:43167039-43167058 None:intergenic 45.0%
! GAAGGATTTGCTTCGAGGTT+TGG + chr5.2:43167545-43167564 MS.gene068827:CDS 45.0%
! GGTTTGGGTGAGTTTGAGTT+TGG + chr5.2:43167561-43167580 MS.gene068827:CDS 45.0%
!! ATGAGGAGTGTTGGTTTGAG+AGG - chr5.2:43167030-43167049 None:intergenic 45.0%
AGAGAGGAGGTTAGGGTATG+TGG - chr5.2:43167102-43167121 None:intergenic 50.0%
AGAGGAGGTTAGGGTATGTG+GGG - chr5.2:43167100-43167119 None:intergenic 50.0%
AGAGGCTGTTGTTGTGTGTG+AGG - chr5.2:43167012-43167031 None:intergenic 50.0%
AGCAGACCACGGTGATTCAT+CGG - chr5.2:43167309-43167328 None:intergenic 50.0%
CATCAACAACGCTTCCAGAC+GGG - chr5.2:43167425-43167444 None:intergenic 50.0%
CCATCAACAACGCTTCCAGA+CGG - chr5.2:43167426-43167445 None:intergenic 50.0%
GAGAGGAGGTTAGGGTATGT+GGG - chr5.2:43167101-43167120 None:intergenic 50.0%
GAGGATGAGAGAGGAGGTTA+GGG - chr5.2:43167109-43167128 None:intergenic 50.0%
GAGGCTGTTGTTGTGTGTGA+GGG - chr5.2:43167011-43167030 None:intergenic 50.0%
GGTGAGGAGGTTGTGGAAAA+GGG - chr5.2:43167079-43167098 None:intergenic 50.0%
GTTGTGGAAAAGGGAGAGAG+AGG - chr5.2:43167070-43167089 None:intergenic 50.0%
! GAAGGGAGTGATGGAGAAGA+TGG - chr5.2:43166973-43166992 None:intergenic 50.0%
! GGATTGGAGTATTACCCGTC+TGG + chr5.2:43167408-43167427 MS.gene068827:CDS 50.0%
! GTGTTGTTAGGATGGGAGAG+AGG - chr5.2:43167141-43167160 None:intergenic 50.0%
!! GGAAGCGTTGTTGATGGACT+CGG + chr5.2:43167429-43167448 MS.gene068827:CDS 50.0%
!! GGGCTAGCTTGTGTGTTTCT+TGG + chr5.2:43167490-43167509 MS.gene068827:CDS 50.0%
!!! AGGGATAGGGTTTTGAACGG+CGG - chr5.2:43167202-43167221 None:intergenic 50.0%
ACGGCGTAAGCGCGATTATC+AGG - chr5.2:43167222-43167241 None:intergenic 55.0%
AGGTTAGGGTATGTGGGGTG+AGG - chr5.2:43167095-43167114 None:intergenic 55.0%
ATCCAGCCGATGAATCACCG+TGG + chr5.2:43167300-43167319 MS.gene068827:CDS 55.0%
CAACCAGATCCGCCCTGATT+CGG + chr5.2:43167260-43167279 MS.gene068827:CDS 55.0%
CGGCGTAAGCGCGATTATCA+GGG - chr5.2:43167221-43167240 None:intergenic 55.0%
CTGACACCGTCCAAGAATGG+CGG - chr5.2:43167457-43167476 None:intergenic 55.0%
CTTGAATGCAACCGCCTCGT+CGG - chr5.2:43167337-43167356 None:intergenic 55.0%
GAGTTCAGGGAGGATGAGAG+AGG - chr5.2:43167118-43167137 None:intergenic 55.0%
GATGGGAGAGAGGGAGTTCA+GGG - chr5.2:43167131-43167150 None:intergenic 55.0%
GGAGGATGAGAGAGGAGGTT+AGG - chr5.2:43167110-43167129 None:intergenic 55.0%
GGGTGAGGAGGTTGTGGAAA+AGG - chr5.2:43167080-43167099 None:intergenic 55.0%
TAACCGAATCAGGGCGGATC+TGG - chr5.2:43167266-43167285 None:intergenic 55.0%
TATGTGGGGTGAGGAGGTTG+TGG - chr5.2:43167086-43167105 None:intergenic 55.0%
TTAGGGTATGTGGGGTGAGG+AGG - chr5.2:43167092-43167111 None:intergenic 55.0%
TTCAGGGAGGATGAGAGAGG+AGG - chr5.2:43167115-43167134 None:intergenic 55.0%
!! CCGTCTGGAAGCGTTGTTGA+TGG + chr5.2:43167423-43167442 MS.gene068827:CDS 55.0%
!! GGATGGTGGAAGAGAGAGAG+TGG - chr5.2:43167173-43167192 None:intergenic 55.0%
CGAGGCGGTTGCATTCAAGC+TGG + chr5.2:43167338-43167357 MS.gene068827:CDS 60.0%
CGTCGGAAGACAGCAGACCA+CGG - chr5.2:43167320-43167339 None:intergenic 60.0%
GACCACGGTGATTCATCGGC+TGG - chr5.2:43167305-43167324 None:intergenic 60.0%
GCTGAGGAGGATTTCCGCGA+CGG - chr5.2:43167241-43167260 None:intergenic 60.0%
GGATGGGAGAGAGGGAGTTC+AGG - chr5.2:43167132-43167151 None:intergenic 60.0%
TAATCGCGCTTACGCCGTCG+CGG + chr5.2:43167224-43167243 MS.gene068827:CDS 60.0%
TCTGCTGTCTTCCGACGAGG+CGG + chr5.2:43167323-43167342 MS.gene068827:CDS 60.0%
TGGTCTGCTGTCTTCCGACG+AGG + chr5.2:43167320-43167339 MS.gene068827:CDS 60.0%
!! TCGGTGCCGCCATTCTTGGA+CGG + chr5.2:43167448-43167467 MS.gene068827:CDS 60.0%
!!! TAGGGTTTTGAACGGCGGCG+AGG - chr5.2:43167197-43167216 None:intergenic 60.0%
CAGGGCGGATCTGGTTGCTG+AGG - chr5.2:43167257-43167276 None:intergenic 65.0%
GGCGGATCTGGTTGCTGAGG+AGG - chr5.2:43167254-43167273 None:intergenic 65.0%
GGGAGAGAGGGAGTTCAGGG+AGG - chr5.2:43167128-43167147 None:intergenic 65.0%
TTGAACGGCGGCGAGGAGGA+TGG - chr5.2:43167190-43167209 None:intergenic 65.0%
!! GGACTCGGTGCCGCCATTCT+TGG + chr5.2:43167444-43167463 MS.gene068827:CDS 65.0%
!!! GGTTTTGAACGGCGGCGAGG+AGG - chr5.2:43167194-43167213 None:intergenic 65.0%
AACGGCGGCGAGGAGGATGG+TGG - chr5.2:43167187-43167206 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr5.2 gene 43166901 43167656 43166901 ID=MS.gene068827
chr5.2 mRNA 43166901 43167656 43166901 ID=MS.gene068827.t1;Parent=MS.gene068827
chr5.2 exon 43166901 43167656 43166901 ID=MS.gene068827.t1.exon1;Parent=MS.gene068827.t1
chr5.2 CDS 43166901 43167656 43166901 ID=cds.MS.gene068827.t1;Parent=MS.gene068827.t1
Gene Sequence

>MS.gene068827

ATGGAAGATGTGATAACAGAAGCAGCACCACCATCAAGATTACTAGAAGAAGACCTAAACAATTTCACACCATCTTCTCCATCACTCCCTTCACCTTTTCTCCTTTTCCCTCACACACAACAACAGCCTCTCAAACCAACACTCCTCATCATCGCTATCTCTTCTCCCTCTCTCTCCCTTTTCCACAACCTCCTCACCCCACATACCCTAACCTCCTCTCTCATCCTCCCTGAACTCCCTCTCTCCCATCCTAACAACACCATCAACATCCACTCTCTCTCTTCCACCATCCTCCTCGCCGCCGTTCAAAACCCTATCCCTGATAATCGCGCTTACGCCGTCGCGGAAATCCTCCTCAGCAACCAGATCCGCCCTGATTCGGTTATCATACTTGATTCGATCCAGCCGATGAATCACCGTGGTCTGCTGTCTTCCGACGAGGCGGTTGCATTCAAGCTGGAGAGTTTTGCTGAAAGGAAGAAAGCGGATGAGGAGAAATTGTTGAGTGGATTGGAGTATTACCCGTCTGGAAGCGTTGTTGATGGACTCGGTGCCGCCATTCTTGGACGGTGTCAGATTTTGAAAATTAGGGCTAGCTTGTGTGTTTCTTGGCCTCAATTTGATTCAGATGTTGTTTTGTTGTTGAAGGATTTGCTTCGAGGTTTGGGTGAGTTTGAGTTTGGGTTTAGTGATGATGAGGTTTTCAAATTTGGGAGGAGTAAGGATCATGTTTTTCAATCTCACTTGTATATTTGA

Protein sequence

>MS.gene068827.t1

MEDVITEAAPPSRLLEEDLNNFTPSSPSLPSPFLLFPHTQQQPLKPTLLIIAISSPSLSLFHNLLTPHTLTSSLILPELPLSHPNNTINIHSLSSTILLAAVQNPIPDNRAYAVAEILLSNQIRPDSVIILDSIQPMNHRGLLSSDEAVAFKLESFAERKKADEEKLLSGLEYYPSGSVVDGLGAAILGRCQILKIRASLCVSWPQFDSDVVLLLKDLLRGLGEFEFGFSDDEVFKFGRSKDHVFQSHLYI