Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene068829.t1 | KEH27916.1 | 91.5 | 117 | 10 | 0 | 1 | 117 | 131 | 247 | 9.50E-50 | 206.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene068829.t1 | Q6NQI8 | 70.9 | 117 | 34 | 0 | 1 | 117 | 176 | 292 | 3.8e-39 | 162.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene068829.t1 | A0A072UET0 | 91.5 | 117 | 10 | 0 | 1 | 117 | 131 | 247 | 6.9e-50 | 206.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene053960 | MS.gene068829 | 0.828309 | 9.66E-55 | -1.69E-46 |
| MS.gene053962 | MS.gene068829 | 0.890521 | 9.14E-74 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 26 sgRNAs with CRISPR-Local
Find 103 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CGACGCTACTGATGTGTTTC+TGG | 0.166561 | 5.2:+43148586 | MS.gene068829:CDS |
| TGCTTGTTCTTAATATTTAT+TGG | 0.221668 | 5.2:+43150385 | MS.gene068829:CDS |
| GCATCATTTACTACCCATTC+TGG | 0.256348 | 5.2:+43148696 | MS.gene068829:CDS |
| GGTGATTGACACCTTGAAAT+TGG | 0.282939 | 5.2:+43150302 | MS.gene068829:CDS |
| TGATTCTGCACCGCTGTATA+TGG | 0.289604 | 5.2:-43148624 | None:intergenic |
| GCACTCAGGTTTGTTCGAAT+TGG | 0.314114 | 5.2:+43148542 | None:intergenic |
| TGGTCCAAGACAAGACAAAA+AGG | 0.376327 | 5.2:-43148667 | None:intergenic |
| GTTGGTCAAATCTGCGCAAA+AGG | 0.396663 | 5.2:+43150431 | MS.gene068829:CDS |
| CAGATGCTTGTCCAATTTCA+AGG | 0.422664 | 5.2:-43150313 | None:intergenic |
| CTTGAAATTGGACAAGCATC+TGG | 0.449431 | 5.2:+43150314 | MS.gene068829:CDS |
| CATCATTTACTACCCATTCT+GGG | 0.450526 | 5.2:+43148697 | MS.gene068829:CDS |
| ATTGGTGGGTGCTGATGCTT+CGG | 0.460019 | 5.2:+43150403 | MS.gene068829:CDS |
| ACTGATGTGTTTCTGGAGAT+AGG | 0.473929 | 5.2:+43148593 | MS.gene068829:CDS |
| GTTAGTGAAGATATTAGATC+TGG | 0.474457 | 5.2:+43150458 | MS.gene068829:CDS |
| TCTGGGTTCTTCGAAGTACA+AGG | 0.495871 | 5.2:+43148714 | MS.gene068829:CDS |
| TTGTTCTTAATATTTATTGG+TGG | 0.510641 | 5.2:+43150388 | MS.gene068829:CDS |
| TGTTCTTAATATTTATTGGT+GGG | 0.517092 | 5.2:+43150389 | MS.gene068829:CDS |
| GCTGATGCTTCGGATGCTGT+TGG | 0.539579 | 5.2:+43150413 | MS.gene068829:CDS |
| TACTTCGAAGAACCCAGAAT+GGG | 0.559855 | 5.2:-43148709 | None:intergenic |
| TTGGTCAAATCTGCGCAAAA+GGG | 0.564115 | 5.2:+43150432 | MS.gene068829:CDS |
| ACACATCAGTAGCGTCGTGA+AGG | 0.571391 | 5.2:-43148580 | None:intergenic |
| CACATCAGTAGCGTCGTGAA+GGG | 0.597324 | 5.2:-43148579 | None:intergenic |
| GTACTTCGAAGAACCCAGAA+TGG | 0.597437 | 5.2:-43148710 | None:intergenic |
| GTAGTAAATGATGCGCAGTA+TGG | 0.606821 | 5.2:-43148687 | None:intergenic |
| GAAATTGGACAAGCATCTGG+TGG | 0.641961 | 5.2:+43150317 | MS.gene068829:CDS |
| GGCAAAATGTCCATATACAG+CGG | 0.676062 | 5.2:+43148614 | MS.gene068829:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAGTAAAAAAATATTACATT+TGG | + | chr5.2:43149690-43149709 | MS.gene068829:intron | 10.0% |
| !! | AAAATAAAAAAAAAAGAGCA+AGG | - | chr5.2:43149159-43149178 | None:intergenic | 15.0% |
| !! | TATAAGCTTATTAGTATTTA+TGG | + | chr5.2:43149458-43149477 | MS.gene068829:intron | 15.0% |
| !! | TGAAATTCATATTTCAAAAA+AGG | + | chr5.2:43148789-43148808 | MS.gene068829:intron | 15.0% |
| !!! | GATTAAATTAATTTGAACAA+TGG | - | chr5.2:43150244-43150263 | None:intergenic | 15.0% |
| !! | AAATTGAACTTCCAATTTAA+AGG | - | chr5.2:43149093-43149112 | None:intergenic | 20.0% |
| !! | AATTGAACTTCCAATTTAAA+GGG | - | chr5.2:43149092-43149111 | None:intergenic | 20.0% |
| !! | AGGAAAACAAAATGTAAATT+CGG | - | chr5.2:43148757-43148776 | None:intergenic | 20.0% |
| !! | TGCTTGTTCTTAATATTTAT+TGG | + | chr5.2:43150385-43150404 | MS.gene068829:CDS | 20.0% |
| !! | TGTTCTTAATATTTATTGGT+GGG | + | chr5.2:43150389-43150408 | MS.gene068829:CDS | 20.0% |
| !! | TTACCATTGCATAAAAATTA+AGG | - | chr5.2:43149123-43149142 | None:intergenic | 20.0% |
| !! | TTGTTCTTAATATTTATTGG+TGG | + | chr5.2:43150388-43150407 | MS.gene068829:CDS | 20.0% |
| !! | TTTATATAATCACTTAATGC+AGG | + | chr5.2:43149288-43149307 | MS.gene068829:intron | 20.0% |
| !! | TTTCAAACCCAATAAAAAAT+TGG | - | chr5.2:43150092-43150111 | None:intergenic | 20.0% |
| !!! | AATCTGAAGTAGTAAATTTA+TGG | + | chr5.2:43149413-43149432 | MS.gene068829:intron | 20.0% |
| !!! | CAGGTTTTTTTATTCATAAA+CGG | - | chr5.2:43148978-43148997 | None:intergenic | 20.0% |
| !!! | TAGGTTTCCAATTTTTTATT+GGG | + | chr5.2:43150082-43150101 | MS.gene068829:intron | 20.0% |
| !!! | TCTTGATGATTTACTATTAT+AGG | + | chr5.2:43149252-43149271 | MS.gene068829:intron | 20.0% |
| !!! | TTAGGTTTCCAATTTTTTAT+TGG | + | chr5.2:43150081-43150100 | MS.gene068829:intron | 20.0% |
| !!! | TTCTGTTTTGAACATAATTT+TGG | - | chr5.2:43148878-43148897 | None:intergenic | 20.0% |
| !!! | TTTGAAATATGAATTTCAAC+AGG | - | chr5.2:43148787-43148806 | None:intergenic | 20.0% |
| ! | AAATATTACATTTGGCAGTT+AGG | + | chr5.2:43149698-43149717 | MS.gene068829:intron | 25.0% |
| ! | AACAAAATGTAAATTCGGAA+CGG | - | chr5.2:43148752-43148771 | None:intergenic | 25.0% |
| ! | AATAACAATTTGTACAAGTG+TGG | - | chr5.2:43149534-43149553 | None:intergenic | 25.0% |
| ! | AATTTACTACTTCAGATTAC+AGG | - | chr5.2:43149411-43149430 | None:intergenic | 25.0% |
| ! | GAAGAAAACAATTAAACAGA+AGG | - | chr5.2:43150266-43150285 | None:intergenic | 25.0% |
| ! | TACCTTCTTTATCATCTAAA+GGG | - | chr5.2:43148948-43148967 | None:intergenic | 25.0% |
| ! | TTACCTTCTTTATCATCTAA+AGG | - | chr5.2:43148949-43148968 | None:intergenic | 25.0% |
| ! | TTTCTTAAGCAGAAAAAGTT+TGG | + | chr5.2:43149314-43149333 | MS.gene068829:intron | 25.0% |
| !! | AGTCCTTAATTTTTATGCAA+TGG | + | chr5.2:43149117-43149136 | MS.gene068829:intron | 25.0% |
| !! | TCATACTTTTCCCTTTAAAT+TGG | + | chr5.2:43149079-43149098 | MS.gene068829:intron | 25.0% |
| !! | TTATAAGAACTTTTTGTCAG+TGG | - | chr5.2:43149443-43149462 | None:intergenic | 25.0% |
| !!! | AAATCAGCCTTTACTTTTAA+AGG | - | chr5.2:43150040-43150059 | None:intergenic | 25.0% |
| !!! | AATATTTTTGACAGCATTCA+TGG | - | chr5.2:43149944-43149963 | None:intergenic | 25.0% |
| AACCAAGTAAAGTTTGTCTT+TGG | - | chr5.2:43150125-43150144 | None:intergenic | 30.0% | |
| AGACAAACTTTACTTGGTTT+AGG | + | chr5.2:43150126-43150145 | MS.gene068829:intron | 30.0% | |
| AGTTAGAAGCTAATCTAAAC+TGG | - | chr5.2:43149380-43149399 | None:intergenic | 30.0% | |
| ATCATTATATCCTGTAGGAT+AGG | + | chr5.2:43149750-43149769 | MS.gene068829:intron | 30.0% | |
| CAAGCACTTAATTGGTAAAA+TGG | - | chr5.2:43149224-43149243 | None:intergenic | 30.0% | |
| GACAAACTTTACTTGGTTTA+GGG | + | chr5.2:43150127-43150146 | MS.gene068829:intron | 30.0% | |
| GAGAACTCTTTGTTAAGAAT+GGG | + | chr5.2:43149885-43149904 | MS.gene068829:intron | 30.0% | |
| GTTAGAAGCTAATCTAAACT+GGG | - | chr5.2:43149379-43149398 | None:intergenic | 30.0% | |
| GTTAGTGAAGATATTAGATC+TGG | + | chr5.2:43150458-43150477 | MS.gene068829:CDS | 30.0% | |
| TAACTAATCATGGATACGTT+AGG | + | chr5.2:43149019-43149038 | MS.gene068829:intron | 30.0% | |
| TAATAATAACTGACTGCTAC+TGG | - | chr5.2:43149818-43149837 | None:intergenic | 30.0% | |
| TGAATCACAGAATAAACTTC+AGG | - | chr5.2:43150197-43150216 | None:intergenic | 30.0% | |
| TGATATCCAAGTCTGATATT+AGG | + | chr5.2:43150063-43150082 | MS.gene068829:intron | 30.0% | |
| TGGAAATCATTATATCCTGT+AGG | + | chr5.2:43149745-43149764 | MS.gene068829:intron | 30.0% | |
| TTCCCTTTAGATGATAAAGA+AGG | + | chr5.2:43148943-43148962 | MS.gene068829:intron | 30.0% | |
| TTGAAGCAGCAAAATATGAA+TGG | - | chr5.2:43149630-43149649 | None:intergenic | 30.0% | |
| ! | CATTTTACCAATTAAGTGCT+TGG | + | chr5.2:43149222-43149241 | MS.gene068829:intron | 30.0% |
| ! | TGATTTTCCAAGCACTTAAT+TGG | - | chr5.2:43149232-43149251 | None:intergenic | 30.0% |
| ! | TTATCCTTTTTGTCTTGTCT+TGG | + | chr5.2:43148663-43148682 | MS.gene068829:CDS | 30.0% |
| ! | TTTCTGGCCTTTAAAAGTAA+AGG | + | chr5.2:43150030-43150049 | MS.gene068829:intron | 30.0% |
| AGCCAAAGACAAACTTTACT+TGG | + | chr5.2:43150120-43150139 | MS.gene068829:intron | 35.0% | |
| AGGATAGGATGAGATTATGA+TGG | + | chr5.2:43149765-43149784 | MS.gene068829:intron | 35.0% | |
| ATAATCTCATCCTATCCTAC+AGG | - | chr5.2:43149763-43149782 | None:intergenic | 35.0% | |
| CACGTAGACAAAATATCAAC+AGG | - | chr5.2:43148997-43149016 | None:intergenic | 35.0% | |
| CATCATTTACTACCCATTCT+GGG | + | chr5.2:43148697-43148716 | MS.gene068829:CDS | 35.0% | |
| CCAGAAATAACATGTCAAGA+AGG | - | chr5.2:43150017-43150036 | None:intergenic | 35.0% | |
| CGCTTCTTCAGAATATGAAT+AGG | - | chr5.2:43148830-43148849 | None:intergenic | 35.0% | |
| CTTAAGCAGAAAAAGTTTGG+TGG | + | chr5.2:43149317-43149336 | MS.gene068829:intron | 35.0% | |
| GAAATGTATCCAAGCTAACT+AGG | + | chr5.2:43149600-43149619 | MS.gene068829:intron | 35.0% | |
| GAATTTCAACAGGTGACAAA+AGG | - | chr5.2:43148777-43148796 | None:intergenic | 35.0% | |
| GGAGAACTCTTTGTTAAGAA+TGG | + | chr5.2:43149884-43149903 | MS.gene068829:intron | 35.0% | |
| GGATAGGATGAGATTATGAT+GGG | + | chr5.2:43149766-43149785 | MS.gene068829:intron | 35.0% | |
| TCTACGTGCTTAACTAATCA+TGG | + | chr5.2:43149009-43149028 | MS.gene068829:intron | 35.0% | |
| TGGAAACCTAATATCAGACT+TGG | - | chr5.2:43150072-43150091 | None:intergenic | 35.0% | |
| ! | CTGCATTGTAAGTCTGAAAT+TGG | + | chr5.2:43149863-43149882 | MS.gene068829:intron | 35.0% |
| !! | CCTTCTTGACATGTTATTTC+TGG | + | chr5.2:43150014-43150033 | MS.gene068829:intron | 35.0% |
| !!! | TCTTCTTTTTTGCAGCTATG+AGG | + | chr5.2:43150281-43150300 | MS.gene068829:intron | 35.0% |
| AAACTGAGAGGTCAGGATTA+AGG | + | chr5.2:43149648-43149667 | MS.gene068829:intron | 40.0% | |
| AATGGATGACCTAGTTAGCT+TGG | - | chr5.2:43149612-43149631 | None:intergenic | 40.0% | |
| CAGATGCTTGTCCAATTTCA+AGG | - | chr5.2:43150316-43150335 | None:intergenic | 40.0% | |
| CTTGAAATTGGACAAGCATC+TGG | + | chr5.2:43150314-43150333 | MS.gene068829:CDS | 40.0% | |
| GAGGACATTGTGTGTATGTT+TGG | + | chr5.2:43149725-43149744 | MS.gene068829:intron | 40.0% | |
| GCATCATTTACTACCCATTC+TGG | + | chr5.2:43148696-43148715 | MS.gene068829:CDS | 40.0% | |
| GGCAAAATGTCCATATACAG+CGG | + | chr5.2:43148614-43148633 | MS.gene068829:CDS | 40.0% | |
| GGTGATTGACACCTTGAAAT+TGG | + | chr5.2:43150302-43150321 | MS.gene068829:CDS | 40.0% | |
| GTAGTAAATGATGCGCAGTA+TGG | - | chr5.2:43148690-43148709 | None:intergenic | 40.0% | |
| TACTTCGAAGAACCCAGAAT+GGG | - | chr5.2:43148712-43148731 | None:intergenic | 40.0% | |
| TGGTCCAAGACAAGACAAAA+AGG | - | chr5.2:43148670-43148689 | None:intergenic | 40.0% | |
| TTGGTCAAATCTGCGCAAAA+GGG | + | chr5.2:43150432-43150451 | MS.gene068829:CDS | 40.0% | |
| ! | ATTCTGAAGAAGCGACCATT+GGG | + | chr5.2:43148836-43148855 | MS.gene068829:intron | 40.0% |
| ! | TATTCTGAAGAAGCGACCAT+TGG | + | chr5.2:43148835-43148854 | MS.gene068829:intron | 40.0% |
| !! | ACTGATGTGTTTCTGGAGAT+AGG | + | chr5.2:43148593-43148612 | MS.gene068829:CDS | 40.0% |
| !! | AGAAGAGTACTTATCCCCAA+TGG | - | chr5.2:43148854-43148873 | None:intergenic | 40.0% |
| !! | TTTTGCTGCTTCAAACTGAG+AGG | + | chr5.2:43149636-43149655 | MS.gene068829:intron | 40.0% |
| !!! | ACAGCTCTAGCGTTACTTTT+GGG | - | chr5.2:43150221-43150240 | None:intergenic | 40.0% |
| CATTTGGCAGTTAGGCTAAG+AGG | + | chr5.2:43149706-43149725 | MS.gene068829:intron | 45.0% | |
| GAAATTGGACAAGCATCTGG+TGG | + | chr5.2:43150317-43150336 | MS.gene068829:CDS | 45.0% | |
| GTACTTCGAAGAACCCAGAA+TGG | - | chr5.2:43148713-43148732 | None:intergenic | 45.0% | |
| GTTGGTCAAATCTGCGCAAA+AGG | + | chr5.2:43150431-43150450 | MS.gene068829:CDS | 45.0% | |
| TCTGGGTTCTTCGAAGTACA+AGG | + | chr5.2:43148714-43148733 | MS.gene068829:CDS | 45.0% | |
| TGATTCTGCACCGCTGTATA+TGG | - | chr5.2:43148627-43148646 | None:intergenic | 45.0% | |
| ! | TTCTGAAGAAGCGACCATTG+GGG | + | chr5.2:43148837-43148856 | MS.gene068829:intron | 45.0% |
| !! | GACAGCTCTAGCGTTACTTT+TGG | - | chr5.2:43150222-43150241 | None:intergenic | 45.0% |
| ACACATCAGTAGCGTCGTGA+AGG | - | chr5.2:43148583-43148602 | None:intergenic | 50.0% | |
| CACATCAGTAGCGTCGTGAA+GGG | - | chr5.2:43148582-43148601 | None:intergenic | 50.0% | |
| CGACGCTACTGATGTGTTTC+TGG | + | chr5.2:43148586-43148605 | MS.gene068829:CDS | 50.0% | |
| CTGCTTCAAACTGAGAGGTC+AGG | + | chr5.2:43149641-43149660 | MS.gene068829:intron | 50.0% | |
| !! | ATTGGTGGGTGCTGATGCTT+CGG | + | chr5.2:43150403-43150422 | MS.gene068829:CDS | 50.0% |
| GCTGATGCTTCGGATGCTGT+TGG | + | chr5.2:43150413-43150432 | MS.gene068829:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.2 | gene | 43148550 | 43150479 | 43148550 | ID=MS.gene068829 |
| chr5.2 | mRNA | 43148550 | 43150479 | 43148550 | ID=MS.gene068829.t1;Parent=MS.gene068829 |
| chr5.2 | exon | 43148550 | 43148735 | 43148550 | ID=MS.gene068829.t1.exon1;Parent=MS.gene068829.t1 |
| chr5.2 | CDS | 43148550 | 43148735 | 43148550 | ID=cds.MS.gene068829.t1;Parent=MS.gene068829.t1 |
| chr5.2 | exon | 43150296 | 43150479 | 43150296 | ID=MS.gene068829.t1.exon2;Parent=MS.gene068829.t1 |
| chr5.2 | CDS | 43150296 | 43150479 | 43150296 | ID=cds.MS.gene068829.t1;Parent=MS.gene068829.t1 |
| Gene Sequence |
| Protein sequence |
