AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene068883

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene068883.t1 MTR_7g115550 89.765 723 44 2 4 697 8 729 0.0 1227
MS.gene068883.t1 MTR_7g115550 89.722 720 44 2 4 694 8 726 0.0 1226
MS.gene068883.t1 MTR_5g010470 82.030 729 52 6 1 696 1 683 0.0 1081
MS.gene068883.t1 MTR_7g061330 38.095 105 64 1 4 107 62 166 6.36e-15 78.6
MS.gene068883.t1 MTR_7g061330 31.008 129 78 2 4 132 114 231 5.78e-11 66.2
MS.gene068883.t1 MTR_7g461410 38.095 105 64 1 4 107 62 166 6.36e-15 78.6
MS.gene068883.t1 MTR_7g461410 31.008 129 78 2 4 132 114 231 5.78e-11 66.2
MS.gene068883.t1 MTR_7g061330 38.095 105 64 1 4 107 26 130 7.68e-15 78.2
MS.gene068883.t1 MTR_7g061330 31.008 129 78 2 4 132 78 195 6.71e-11 65.9
MS.gene068883.t1 MTR_8g468380 34.021 97 64 0 3 99 22 118 6.77e-13 71.6
MS.gene068883.t1 MTR_3g110028 34.286 105 68 1 4 107 34 138 1.14e-12 72.0
MS.gene068883.t1 MTR_3g110028 36.471 85 54 0 4 88 86 170 3.94e-12 70.1
MS.gene068883.t1 MTR_5g038910 33.945 109 68 1 3 111 26 130 1.40e-12 70.9
MS.gene068883.t1 MTR_5g038910 33.945 109 68 1 3 111 26 130 2.00e-12 70.1
MS.gene068883.t1 MTR_1g026870 34.343 99 64 1 4 101 34 132 2.13e-12 71.2
MS.gene068883.t1 MTR_7g451170 37.647 85 53 0 4 88 192 276 5.19e-12 68.9
MS.gene068883.t1 MTR_7g035350 33.981 103 65 1 5 104 227 329 1.19e-11 67.8
MS.gene068883.t1 MTR_4g088015 35.294 85 55 0 4 88 222 306 1.26e-11 67.8
MS.gene068883.t1 MTR_7g037260 36.471 85 54 0 4 88 146 230 2.74e-11 66.2
MS.gene068883.t1 MTR_6g027340 34.783 92 60 0 6 97 19 110 3.45e-11 63.5
MS.gene068883.t1 MTR_4g105660 37.647 85 53 0 4 88 181 265 4.52e-11 65.9
MS.gene068883.t1 MTR_6g027360 35.052 97 63 0 1 97 76 172 5.66e-11 64.7
MS.gene068883.t1 MTR_1g057980 32.258 93 63 0 4 96 81 173 6.91e-11 64.3
MS.gene068883.t1 MTR_5g010650 37.349 83 52 0 6 88 113 195 6.99e-11 65.5
MS.gene068883.t1 MTR_6g027370 31.959 97 66 0 4 100 100 196 8.60e-11 64.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene068883.t1 AT1G09770 71.468 722 171 5 4 693 8 726 0.0 968
MS.gene068883.t1 AT5G02320 37.143 105 65 1 4 107 76 180 1.48e-14 77.4
MS.gene068883.t1 AT5G02320 34.118 85 56 0 4 88 128 212 7.84e-11 65.9
MS.gene068883.t1 AT5G02320 37.143 105 65 1 4 107 76 180 1.48e-14 77.4
MS.gene068883.t1 AT5G02320 34.118 85 56 0 4 88 128 212 7.84e-11 65.9
MS.gene068883.t1 AT5G02320 37.143 105 65 1 4 107 16 120 1.58e-14 77.0
MS.gene068883.t1 AT3G18100 34.375 96 63 0 4 99 547 642 1.05e-13 75.5
MS.gene068883.t1 AT3G18100 36.082 97 60 2 6 101 498 593 2.69e-11 67.4
MS.gene068883.t1 AT3G09370 36.190 105 66 1 4 107 84 188 1.23e-13 74.3
MS.gene068883.t1 AT3G09370 36.190 105 66 1 4 107 79 183 1.29e-13 74.3
MS.gene068883.t1 AT3G09370 36.190 105 66 1 4 107 92 196 1.51e-13 74.3
MS.gene068883.t1 AT3G18100 34.375 96 63 0 4 99 499 594 1.58e-13 74.7
MS.gene068883.t1 AT3G18100 36.082 97 60 2 6 101 450 545 4.16e-11 67.0
MS.gene068883.t1 AT5G11510 37.374 99 61 1 4 101 30 128 1.66e-13 74.7
MS.gene068883.t1 AT5G11510 37.374 99 61 1 4 101 30 128 1.66e-13 74.7
MS.gene068883.t1 AT5G11510 37.374 99 61 1 4 101 30 128 1.66e-13 74.7
MS.gene068883.t1 AT3G18100 34.375 96 63 0 4 99 386 481 2.10e-13 74.3
MS.gene068883.t1 AT3G18100 36.082 97 60 2 6 101 337 432 5.92e-11 66.2
MS.gene068883.t1 AT3G18100 34.375 96 63 0 4 99 334 429 2.47e-13 73.9
MS.gene068883.t1 AT3G18100 36.082 97 60 2 6 101 285 380 7.47e-11 65.9
MS.gene068883.t1 AT5G11510 37.374 99 61 1 4 101 30 128 2.84e-13 73.9
MS.gene068883.t1 AT5G11510 37.374 99 61 1 4 101 30 128 2.93e-13 73.9
MS.gene068883.t1 AT4G18770 38.824 85 52 0 4 88 218 302 8.43e-13 71.6
MS.gene068883.t1 AT4G32730 35.354 99 63 1 4 101 36 134 1.85e-12 71.2
MS.gene068883.t1 AT4G32730 35.354 99 63 1 4 101 36 134 1.85e-12 71.2
MS.gene068883.t1 AT4G32730 35.354 99 63 1 4 101 36 134 1.85e-12 71.2
MS.gene068883.t1 AT4G32730 35.354 99 63 1 4 101 36 134 1.85e-12 71.2
MS.gene068883.t1 AT4G32730 35.354 99 63 1 4 101 36 134 2.78e-12 70.9
MS.gene068883.t1 AT4G33450 32.990 97 65 0 4 100 20 116 4.85e-12 67.0
MS.gene068883.t1 AT5G40360 36.471 85 54 0 4 88 159 243 6.05e-12 68.2
MS.gene068883.t1 AT2G02820 30.579 121 70 1 3 109 30 150 8.70e-12 68.6
MS.gene068883.t1 AT2G02820 30.579 121 70 1 3 109 30 150 1.25e-11 67.8
MS.gene068883.t1 AT4G00540 37.500 112 61 2 4 106 47 158 1.32e-11 67.4
MS.gene068883.t1 AT4G00540 37.500 112 61 2 4 106 45 156 1.72e-11 67.4
MS.gene068883.t1 AT2G02820 30.579 121 70 1 3 109 30 150 1.96e-11 66.6
MS.gene068883.t1 AT1G14350 31.959 97 66 0 3 99 25 121 2.48e-11 67.0
MS.gene068883.t1 AT1G14350 31.959 97 66 0 3 99 25 121 2.48e-11 67.0
MS.gene068883.t1 AT4G38620 31.655 139 84 5 4 138 15 146 3.13e-11 65.1
MS.gene068883.t1 AT1G17950 31.313 99 68 0 2 100 4 102 3.55e-11 64.7
MS.gene068883.t1 AT5G17800 34.343 99 65 0 2 100 92 190 4.20e-11 65.5
MS.gene068883.t1 AT5G14750 33.708 89 58 1 4 91 19 107 8.58e-11 62.4

Find 195 sgRNAs with CRISPR-Local

Find 256 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GATCTCTTTCCAACTGTTTC+TGG 0.013419 2.1:-46526181 None:intergenic
TTCTGGGTCATTCAGCTTCT+TGG 0.153986 2.1:-46526164 None:intergenic
TGCCAGCGCCATACCACTTC+TGG 0.197747 2.1:-46526582 None:intergenic
ATTTCATAACAGCAGCTTTC+AGG 0.225007 2.1:-46525373 None:intergenic
CAGCATCTCTGGAGCTTATT+AGG 0.228626 2.1:+46526951 MS.gene068883:CDS
TTGGGTGTCTGTGGATAATT+AGG 0.241982 2.1:-46526334 None:intergenic
CATGATGGAAGCTGAAAATT+TGG 0.248037 2.1:+46526417 MS.gene068883:CDS
AAAGGAGCTTCCAGCCTTAC+TGG 0.273790 2.1:+46527054 MS.gene068883:CDS
GTTCTCTGAGGAGTTCTTAA+AGG 0.283026 2.1:-46526370 None:intergenic
AAAGCTGCTGTTATGAAATA+TGG 0.287568 2.1:+46525377 MS.gene068883:CDS
GAATTGGTTGCATCACTATC+TGG 0.302904 2.1:-46526781 None:intergenic
GGTCGCGCAGGCTTCGATTC+AGG 0.310060 2.1:-46525716 None:intergenic
AGTCCAATCAGTCTTCTTAA+TGG 0.313583 2.1:-46525489 None:intergenic
GTTTGAGAAAAGGCCTCCTT+TGG 0.314999 2.1:+46525967 MS.gene068883:CDS
CCCTGTCATAGAAGATTATC+AGG 0.315605 2.1:+46527165 MS.gene068883:CDS
CCATTGCCTCACGAAGATAC+TGG 0.317289 2.1:-46527894 None:intergenic
ATCTCTTTCCAACTGTTTCT+GGG 0.330928 2.1:-46526180 None:intergenic
TCTTCAGCATCTTCTTGAAT+TGG 0.331097 2.1:-46526797 None:intergenic
ATCTCGTCGGCTTGCTCAAT+AGG 0.342238 2.1:-46527025 None:intergenic
TCAAGTTCTTCTATAGTAGT+AGG 0.344179 2.1:-46526040 None:intergenic
TAGGCCTCCTCCAGCATCTC+TGG 0.367513 2.1:+46526940 MS.gene068883:CDS
CAGAAATTCCGTTTGAGAAA+AGG 0.368252 2.1:+46525957 MS.gene068883:CDS
GCTTGAGGAGATAGCAAATA+AGG 0.373696 2.1:+46527099 MS.gene068883:CDS
CCTTGACGCCACTTGTGTTA+AGG 0.386161 2.1:+46525631 MS.gene068883:CDS
TCTCGTCGGCTTGCTCAATA+GGG 0.387795 2.1:-46527024 None:intergenic
AGGCCCATTTGCTGCTCGTT+TGG 0.388358 2.1:-46527137 None:intergenic
TCAGATTTCCGATCAAGAAT+TGG 0.389053 2.1:+46526225 MS.gene068883:CDS
GCAGGCTTCGATTCAGGATT+CGG 0.393602 2.1:-46525710 None:intergenic
CCAAAATGAGCATAGGTGTT+TGG 0.397192 2.1:+46525330 None:intergenic
CTACGAAGGGAACTCCTCTC+AGG 0.399722 2.1:+46526630 MS.gene068883:CDS
AGGCTGCTGGGATTGATATA+AGG 0.409355 2.1:+46525897 MS.gene068883:CDS
AGAGATGCTGGAGGAGGCCT+AGG 0.415805 2.1:-46526938 None:intergenic
CATGGTCTATCAAGTGTTGC+TGG 0.417267 2.1:+46528003 MS.gene068883:CDS
GGATCCAAACGAGCAGCAAA+TGG 0.417377 2.1:+46527133 MS.gene068883:CDS
TTGATAGACCATGTGTGTTA+CGG 0.418408 2.1:-46527993 None:intergenic
GCTACATATCAATGAAGATA+TGG 0.420115 2.1:+46526658 MS.gene068883:CDS
TAAGGCAGCGGAGGAGGAAA+AGG 0.424744 2.1:+46525915 MS.gene068883:CDS
TGATAGACCATGTGTGTTAC+GGG 0.426495 2.1:-46527992 None:intergenic
TTGGATGAAATTGCCAAGTT+GGG 0.429250 2.1:+46526244 MS.gene068883:CDS
CTTCCATTAAGAAGACTGAT+TGG 0.431009 2.1:+46525486 MS.gene068883:CDS
AGCTTTCAGGATTTCATCCT+CGG 0.436489 2.1:-46525360 None:intergenic
CAGTATCTTCGTGAGGCAAT+GGG 0.437430 2.1:+46527895 MS.gene068883:CDS
ATTGGATGAAATTGCCAAGT+TGG 0.444138 2.1:+46526243 MS.gene068883:CDS
AAGAACTTGAGGGGAAAAGA+AGG 0.444403 2.1:+46526053 MS.gene068883:CDS
GCCAGCGCCATACCACTTCT+GGG 0.445237 2.1:-46526581 None:intergenic
GCAAATAAGGAGAGAGAGAA+AGG 0.446065 2.1:+46527112 MS.gene068883:CDS
CTAATAAGCTCCAGAGATGC+TGG 0.447893 2.1:-46526950 None:intergenic
ATCCTCTTCAATCTTCTCCT+CGG 0.449369 2.1:-46526822 None:intergenic
CCATACCACTTCTGGGAGTA+AGG 0.450702 2.1:-46526574 None:intergenic
AAGAGAGAATTGAAGGCTGC+TGG 0.451049 2.1:+46525884 MS.gene068883:CDS
TCCTGATAATCTTCTATGAC+AGG 0.451422 2.1:-46527166 None:intergenic
TCATACTCATCCATATCAAC+AGG 0.454291 2.1:-46525743 None:intergenic
ACCCACAATGGTATCACTAA+TGG 0.455710 2.1:-46525570 None:intergenic
AGAAGAAGAGGCACGGCAGA+AGG 0.457675 2.1:+46526874 MS.gene068883:CDS
TCCCCTGGACGCAATTTCCT+AGG 0.458724 2.1:-46525680 None:intergenic
AAAGGAGTCAAGGCTTGGTT+GGG 0.461730 2.1:-46526352 None:intergenic
TGCAGAGAGGCAAGATGCCC+CGG 0.470266 2.1:+46526126 MS.gene068883:CDS
GAGTCTCAGACACCACTGTT+AGG 0.471291 2.1:+46526451 MS.gene068883:CDS
CTGAAAATTTGGCCAGGCTG+AGG 0.471566 2.1:+46526428 MS.gene068883:CDS
TTCTTAAAGGAGTCAAGGCT+TGG 0.471836 2.1:-46526357 None:intergenic
TGGCATGCAGAATAGCAGCC+GGG 0.473211 2.1:-46526144 None:intergenic
TTGATATAAGGCAGCGGAGG+AGG 0.474103 2.1:+46525909 MS.gene068883:CDS
AGCCAACCGAGCACATGCTT+CGG 0.476372 2.1:-46525780 None:intergenic
TGATGTTGCTGATGAGGATA+GGG 0.479449 2.1:+46525997 MS.gene068883:CDS
AATTTCATCCAATTCTTGAT+CGG 0.479560 2.1:-46526233 None:intergenic
GCGACCCGATCCTGTTGATA+TGG 0.481942 2.1:+46525733 MS.gene068883:CDS
CCAGTATCTTCGTGAGGCAA+TGG 0.484875 2.1:+46527894 MS.gene068883:CDS
TGTCTGAGACTCCCTCAGCC+TGG 0.484905 2.1:-46526440 None:intergenic
AACGATCAAAAGTCATTCAA+AGG 0.485643 2.1:+46526909 MS.gene068883:CDS
TCCTCTTCAATCTTCTCCTC+GGG 0.488427 2.1:-46526821 None:intergenic
CTCCTTTCTTATCATCTCGT+CGG 0.493278 2.1:-46527038 None:intergenic
TCATTCAAAGGGAACTTCCT+AGG 0.493709 2.1:+46526921 MS.gene068883:CDS
ACGAGTAGCACTGCTGCCTT+CGG 0.495690 2.1:-46526302 None:intergenic
TTCCGAAGCATGTGCTCGGT+TGG 0.496711 2.1:+46525778 MS.gene068883:CDS
TCTTCTATGACAGGGATAGC+AGG 0.496725 2.1:-46527157 None:intergenic
GTCTCCGTCAGCTCTTATCA+AGG 0.499862 2.1:-46526978 None:intergenic
TTGTCATGCTCCAGTAAGGC+TGG 0.499885 2.1:-46527064 None:intergenic
TGCTTTCCGAAGCATGTGCT+CGG 0.502006 2.1:+46525774 MS.gene068883:CDS
GTGAGAAGTAAGCTGGATGA+TGG 0.502979 2.1:+46528069 MS.gene068883:CDS
CTCATCCATATCAACAGGAT+CGG 0.503902 2.1:-46525738 None:intergenic
AAGTAAGCTGGATGATGGGA+AGG 0.506469 2.1:+46528074 MS.gene068883:CDS
ATAGCATCTCCTTTACTAGC+TGG 0.506651 2.1:-46526394 None:intergenic
AGAGGATATGTCTGATAGAA+TGG 0.507303 2.1:+46526838 MS.gene068883:CDS
AGCTCCAGAGATGCTGGAGG+AGG 0.508047 2.1:-46526944 None:intergenic
GATGATTCGACTTGAGAAGA+AGG 0.511403 2.1:+46528101 MS.gene068883:CDS
TGATAGAATGGCTAGAGAAA+AGG 0.511992 2.1:+46526850 MS.gene068883:CDS
ATTGCTGCCCACAAGGTCAC+TGG 0.512710 2.1:-46526272 None:intergenic
TTGGCATGCAGAATAGCAGC+CGG 0.514383 2.1:-46526145 None:intergenic
TTTGAGAAAAGGCCTCCTTT+GGG 0.515050 2.1:+46525968 MS.gene068883:CDS
CAGAGAACACCAGCTAGTAA+AGG 0.515217 2.1:+46526385 MS.gene068883:CDS
TGCAGATTTGCAAACGAGTA+GGG 0.518174 2.1:-46525426 None:intergenic
CATTGCCTCACGAAGATACT+GGG 0.519621 2.1:-46527893 None:intergenic
TGGGTGTCTGTGGATAATTA+GGG 0.519886 2.1:-46526333 None:intergenic
TTGATGTTGCTGATGAGGAT+AGG 0.524642 2.1:+46525996 MS.gene068883:CDS
TCGGCTTGCTCAATAGGGGT+TGG 0.524733 2.1:-46527019 None:intergenic
TGAAAATTTGGCCAGGCTGA+GGG 0.525084 2.1:+46526429 MS.gene068883:CDS
GACCCTAGGAAATTGCGTCC+AGG 0.525314 2.1:+46525677 MS.gene068883:CDS
CTGCAGATTTGCAAACGAGT+AGG 0.526600 2.1:-46525427 None:intergenic
AGCATTGTCATGCTCCAGTA+AGG 0.527412 2.1:-46527068 None:intergenic
ACCATTAGTGATACCATTGT+GGG 0.527710 2.1:+46525569 MS.gene068883:CDS
CAAGTTCTTCTATAGTAGTA+GGG 0.528361 2.1:-46526039 None:intergenic
TAAAGGAGTCAAGGCTTGGT+TGG 0.528607 2.1:-46526353 None:intergenic
GAAGTGGTATGGCGCTGGCA+AGG 0.528663 2.1:+46526585 MS.gene068883:CDS
TGAGAATGTGAGAAGTAAGC+TGG 0.529343 2.1:+46528062 MS.gene068883:CDS
GCTTGCTCAATAGGGGTTGG+TGG 0.531592 2.1:-46527016 None:intergenic
TCATCCATATCAACAGGATC+GGG 0.534614 2.1:-46525737 None:intergenic
ACCCTAGGAAATTGCGTCCA+GGG 0.536757 2.1:+46525678 MS.gene068883:CDS
AAGTGGTATGGCGCTGGCAA+GGG 0.537683 2.1:+46526586 MS.gene068883:CDS
TCCCAGAAGTGGTATGGCGC+TGG 0.538783 2.1:+46526580 MS.gene068883:CDS
GATCCAAACGAGCAGCAAAT+GGG 0.542547 2.1:+46527134 MS.gene068883:CDS
AGAGAGAATTGAAGGCTGCT+GGG 0.549517 2.1:+46525885 MS.gene068883:CDS
TGATCGGAAATCTGAGGTGG+TGG 0.551298 2.1:-46526217 None:intergenic
CCTTACTCCCAGAAGTGGTA+TGG 0.551739 2.1:+46526574 MS.gene068883:CDS
AGCCGACGAGATGATAAGAA+AGG 0.552168 2.1:+46527036 MS.gene068883:CDS
GAAAAGAAGGATTGATGTTG+AGG 0.552508 2.1:+46526066 MS.gene068883:CDS
GGTTATGCCAGTGACCTTGT+GGG 0.556103 2.1:+46526265 MS.gene068883:CDS
TGAGAAAGCAAGACGTTGCA+AGG 0.556741 2.1:+46526095 MS.gene068883:CDS
GGGTTATGCCAGTGACCTTG+TGG 0.560009 2.1:+46526264 MS.gene068883:CDS
GGATTCGGATCAATCTCCCC+TGG 0.561665 2.1:-46525695 None:intergenic
CCCCTGGACGCAATTTCCTA+GGG 0.562986 2.1:-46525679 None:intergenic
AGAAACAGCTTGAGGAAGCA+AGG 0.565360 2.1:+46525840 MS.gene068883:CDS
TCAACAGGATCGGGTCGCGC+AGG 0.566355 2.1:-46525728 None:intergenic
ACGATCAAAAGTCATTCAAA+GGG 0.567785 2.1:+46526910 MS.gene068883:CDS
AGGAGTTCTTAAAGGAGTCA+AGG 0.570143 2.1:-46526362 None:intergenic
ACTACTATAGAAGAACTTGA+GGG 0.571318 2.1:+46526043 MS.gene068883:CDS
TAAAGGAGATGCTATCATGA+TGG 0.573963 2.1:+46526402 MS.gene068883:CDS
CTGAATGACCCAGAAACAGT+TGG 0.574442 2.1:+46526172 MS.gene068883:CDS
CAATCAGTCTTCTTAATGGA+AGG 0.575954 2.1:-46525485 None:intergenic
TTACTAGCTGGTGTTCTCTG+AGG 0.578802 2.1:-46526382 None:intergenic
TAGAGAAAAGGCAGAAGAAG+AGG 0.579560 2.1:+46526862 MS.gene068883:CDS
ATGAACCTGGTGATGACCCT+AGG 0.580871 2.1:+46525663 MS.gene068883:CDS
TTATTTGCTATCTCCTCAAG+CGG 0.583374 2.1:-46527097 None:intergenic
GTCACTGGCATAACCCAACT+TGG 0.585958 2.1:-46526257 None:intergenic
AAGGTTACATTACTCACGCA+AGG 0.586053 2.1:+46528120 MS.gene068883:CDS
GTAAAGCTCGTTGGTATGAG+TGG 0.588526 2.1:+46525456 MS.gene068883:CDS
AACTCCTTGATAAGAGCTGA+CGG 0.589304 2.1:+46526974 MS.gene068883:CDS
AAGGCTTGGTTGGGTGTCTG+TGG 0.589342 2.1:-46526343 None:intergenic
CCTGATAATCTTCTATGACA+GGG 0.589597 2.1:-46527165 None:intergenic
TGCTTCAACAAAATCCTCGA+GGG 0.592155 2.1:-46527932 None:intergenic
CCTTAACACAAGTGGCGTCA+AGG 0.592424 2.1:-46525631 None:intergenic
GGTATGGCGCTGGCAAGGGA+TGG 0.593619 2.1:+46526590 MS.gene068883:CDS
CTCCTTCGCAGTGTCTTGAA+CGG 0.594673 2.1:+46525597 MS.gene068883:CDS
TGCTGATGAGGATAGGGAGG+TGG 0.595277 2.1:+46526003 MS.gene068883:CDS
CAGAACGGTGTAAAGCTCGT+TGG 0.595953 2.1:+46525447 MS.gene068883:CDS
AGTGTTGCCAAAATGAGCAT+AGG 0.596521 2.1:+46525323 None:intergenic
AACCATTAGTGATACCATTG+TGG 0.597390 2.1:+46525568 MS.gene068883:CDS
TGAGAAGTAAGCTGGATGAT+GGG 0.598085 2.1:+46528070 MS.gene068883:CDS
TACTACTATAGAAGAACTTG+AGG 0.598439 2.1:+46526042 MS.gene068883:CDS
AAAAGGCAGAAGAAGAGGCA+CGG 0.599691 2.1:+46526867 MS.gene068883:CDS
GACTGATTGGACTCGAGAAG+AGG 0.600369 2.1:+46525499 MS.gene068883:CDS
TGAGTTCATTGCTGCCCACA+AGG 0.602663 2.1:-46526279 None:intergenic
CTAATGGTTCTCCACTGAGT+AGG 0.603402 2.1:-46525554 None:intergenic
TGGAAGCTGAAAATTTGGCC+AGG 0.607474 2.1:+46526422 MS.gene068883:CDS
GCTCGGTTGGCTAACACGAA+AGG 0.608258 2.1:+46525791 MS.gene068883:CDS
AGCGGAGGAGGAAAAGGAGA+GGG 0.609580 2.1:+46525921 MS.gene068883:CDS
CAATGCTAAGTATCCGCTTG+AGG 0.613956 2.1:+46527084 MS.gene068883:CDS
TACGAAGGGAACTCCTCTCA+GGG 0.614373 2.1:+46526631 MS.gene068883:CDS
AATTTCCTAGGGTCATCACC+AGG 0.619740 2.1:-46525668 None:intergenic
CAGCGGAGGAGGAAAAGGAG+AGG 0.619802 2.1:+46525920 MS.gene068883:CDS
CTGGGATTGATATAAGGCAG+CGG 0.621106 2.1:+46525903 MS.gene068883:CDS
GTTATCGTCCTTAACACAAG+TGG 0.622208 2.1:-46525639 None:intergenic
GCAGGCCTTACTCCCAGAAG+TGG 0.624004 2.1:+46526569 MS.gene068883:intron
CGAAGGAGTACGACCCACAA+TGG 0.624367 2.1:-46525582 None:intergenic
CCCTAGGAAATTGCGTCCAG+GGG 0.631019 2.1:+46525679 MS.gene068883:CDS
CTCGTCGGCTTGCTCAATAG+GGG 0.633774 2.1:-46527023 None:intergenic
TTTGATACCCGTAACACACA+TGG 0.638610 2.1:+46527985 MS.gene068883:CDS
GGCATGCAGAATAGCAGCCG+GGG 0.639810 2.1:-46526143 None:intergenic
AAGGACGATAACTATGAACC+TGG 0.641325 2.1:+46525650 MS.gene068883:CDS
TGTTGCTGATGAGGATAGGG+AGG 0.645550 2.1:+46526000 MS.gene068883:CDS
GCAACATCAAAATAACCCAA+AGG 0.645993 2.1:-46525983 None:intergenic
AGAAGATGCTGAAGAGCCCG+AGG 0.646686 2.1:+46526805 MS.gene068883:CDS
CTACTATAGAAGAACTTGAG+GGG 0.654103 2.1:+46526044 MS.gene068883:CDS
GCCCGAGGAGAAGATTGAAG+AGG 0.654306 2.1:+46526820 MS.gene068883:CDS
AATTCTTGATCGGAAATCTG+AGG 0.665249 2.1:-46526223 None:intergenic
AGCTAGGGAGAAACAGCTTG+AGG 0.665565 2.1:+46525832 MS.gene068883:CDS
TACCGTTCAAGACACTGCGA+AGG 0.666095 2.1:-46525599 None:intergenic
AGGTTACATTACTCACGCAA+GGG 0.667391 2.1:+46528121 MS.gene068883:CDS
GGCAGCAATGAACTCACCGA+AGG 0.667861 2.1:+46526286 MS.gene068883:CDS
CTAAGCTTACGCCTACTCAG+TGG 0.671805 2.1:+46525543 MS.gene068883:CDS
TCGTTTGCAAATCTGCAGAA+CGG 0.675444 2.1:+46525432 MS.gene068883:CDS
TCTTGATCGGAAATCTGAGG+TGG 0.678038 2.1:-46526220 None:intergenic
ACATCAAAATAACCCAAAGG+AGG 0.679868 2.1:-46525980 None:intergenic
TGAAGCCCAGTATCTTCGTG+AGG 0.688109 2.1:+46527888 MS.gene068883:CDS
GTGCTTCAACAAAATCCTCG+AGG 0.688213 2.1:-46527933 None:intergenic
ATATGTAGCTCATCCCTGAG+AGG 0.688581 2.1:-46526644 None:intergenic
AGTATCTTCGTGAGGCAATG+GGG 0.691770 2.1:+46527896 MS.gene068883:CDS
GGATTGATATAAGGCAGCGG+AGG 0.699913 2.1:+46525906 MS.gene068883:CDS
ATAAGCTCCAGAGATGCTGG+AGG 0.701115 2.1:-46526947 None:intergenic
AACAGCTTGAGGAAGCAAGG+AGG 0.704973 2.1:+46525843 MS.gene068883:CDS
AAGGATGCAACTCCTAACAG+TGG 0.713627 2.1:-46526463 None:intergenic
TGATATGGATGAGTATGAGA+AGG 0.714958 2.1:+46525748 MS.gene068883:CDS
TGTCATAGAAGATTATCAGG+AGG 0.727087 2.1:+46527168 MS.gene068883:CDS
GCATGAAAATGATTCCCTCG+AGG 0.731393 2.1:+46527918 MS.gene068883:CDS
CAAGGAATAAAATTGCAGAG+AGG 0.745082 2.1:+46526113 MS.gene068883:CDS
AGGTGTTTGGACAAACACCG+AGG 0.789261 2.1:+46525343 MS.gene068883:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AGGCATAAAAATAAAATTAA+AGG - chr2.1:46527235-46527254 None:intergenic 15.0%
!! ATAATAATTAACAAACAGTT+TGG - chr2.1:46527750-46527769 None:intergenic 15.0%
!! ATTATAACTGAAAAAAAACA+AGG - chr2.1:46527576-46527595 None:intergenic 15.0%
!! AAGTTTGTATTACTATCATT+CGG - chr2.1:46527727-46527746 None:intergenic 20.0%
! AATTTCATCCAATTCTTGAT+CGG - chr2.1:46526236-46526255 None:intergenic 25.0%
! GAAATATGGTAAAAATCAGT+GGG + chr2.1:46525391-46525410 MS.gene068883:CDS 25.0%
! GATGCCAAGTAATTAATAAT+CGG - chr2.1:46527396-46527415 None:intergenic 25.0%
! TAGGGAATAAATGTTGTAAA+AGG - chr2.1:46527690-46527709 None:intergenic 25.0%
! TGAAATATGGTAAAAATCAG+TGG + chr2.1:46525390-46525409 MS.gene068883:CDS 25.0%
! TTGTCCGATTATTAATTACT+TGG + chr2.1:46527389-46527408 MS.gene068883:intron 25.0%
!! ATGTGAATTTCCTTTTTCTT+AGG - chr2.1:46526509-46526528 None:intergenic 25.0%
!! ATTTTTACATAAGATTCCAG+TGG - chr2.1:46527458-46527477 None:intergenic 25.0%
!! TTTTATCTGTTGTTCTCAAT+AGG + chr2.1:46527846-46527865 MS.gene068883:intron 25.0%
AAAAAGCAAAAAGGAAAGCT+AGG + chr2.1:46525816-46525835 MS.gene068883:CDS 30.0%
AAAAGCAAAAAGGAAAGCTA+GGG + chr2.1:46525817-46525836 MS.gene068883:CDS 30.0%
AAAGCTGCTGTTATGAAATA+TGG + chr2.1:46525377-46525396 MS.gene068883:CDS 30.0%
AACGATCAAAAGTCATTCAA+AGG + chr2.1:46526909-46526928 MS.gene068883:CDS 30.0%
AATATGCAACTAGCTCATAA+AGG - chr2.1:46527524-46527543 None:intergenic 30.0%
ACAGAAAAAGAGAGAATTGA+AGG + chr2.1:46525877-46525896 MS.gene068883:CDS 30.0%
ACGATCAAAAGTCATTCAAA+GGG + chr2.1:46526910-46526929 MS.gene068883:CDS 30.0%
ACTACTATAGAAGAACTTGA+GGG + chr2.1:46526043-46526062 MS.gene068883:CDS 30.0%
ATGAAGAAAAGCGAAGTAAA+AGG - chr2.1:46527255-46527274 None:intergenic 30.0%
CAAGTTCTTCTATAGTAGTA+GGG - chr2.1:46526042-46526061 None:intergenic 30.0%
CATCTCCATAGTCAAATTTA+AGG - chr2.1:46527779-46527798 None:intergenic 30.0%
CGAAAGGTAAAAAAGCAAAA+AGG + chr2.1:46525807-46525826 MS.gene068883:CDS 30.0%
GCTACATATCAATGAAGATA+TGG + chr2.1:46526658-46526677 MS.gene068883:CDS 30.0%
TACTACTATAGAAGAACTTG+AGG + chr2.1:46526042-46526061 MS.gene068883:CDS 30.0%
TATGAGCTAGTTGCATATTA+TGG + chr2.1:46527525-46527544 MS.gene068883:intron 30.0%
TCAAGTTCTTCTATAGTAGT+AGG - chr2.1:46526043-46526062 None:intergenic 30.0%
TGACTCCTTAAATTTGACTA+TGG + chr2.1:46527771-46527790 MS.gene068883:intron 30.0%
!! ACTATCTGGTACTCATTTTT+AGG - chr2.1:46526770-46526789 None:intergenic 30.0%
!!! TTATTTTGATGTTGCTGATG+AGG + chr2.1:46525991-46526010 MS.gene068883:CDS 30.0%
AACCATTAGTGATACCATTG+TGG + chr2.1:46525568-46525587 MS.gene068883:CDS 35.0%
AAGATTCCAGTGGTTTCAAA+GGG - chr2.1:46527448-46527467 None:intergenic 35.0%
AATTCTTGATCGGAAATCTG+AGG - chr2.1:46526226-46526245 None:intergenic 35.0%
ACATCAAAATAACCCAAAGG+AGG - chr2.1:46525983-46526002 None:intergenic 35.0%
ACCATTAGTGATACCATTGT+GGG + chr2.1:46525569-46525588 MS.gene068883:CDS 35.0%
AGAGGATATGTCTGATAGAA+TGG + chr2.1:46526838-46526857 MS.gene068883:CDS 35.0%
AGTCCAATCAGTCTTCTTAA+TGG - chr2.1:46525492-46525511 None:intergenic 35.0%
ATTGGATGAAATTGCCAAGT+TGG + chr2.1:46526243-46526262 MS.gene068883:CDS 35.0%
ATTTCATAACAGCAGCTTTC+AGG - chr2.1:46525376-46525395 None:intergenic 35.0%
CAAGGAATAAAATTGCAGAG+AGG + chr2.1:46526113-46526132 MS.gene068883:CDS 35.0%
CAATCAGTCTTCTTAATGGA+AGG - chr2.1:46525488-46525507 None:intergenic 35.0%
CAGAAATTCCGTTTGAGAAA+AGG + chr2.1:46525957-46525976 MS.gene068883:CDS 35.0%
CCTGATAATCTTCTATGACA+GGG - chr2.1:46527168-46527187 None:intergenic 35.0%
CTACTATAGAAGAACTTGAG+GGG + chr2.1:46526044-46526063 MS.gene068883:CDS 35.0%
CTTCCATTAAGAAGACTGAT+TGG + chr2.1:46525486-46525505 MS.gene068883:CDS 35.0%
GAAAAGAAGGATTGATGTTG+AGG + chr2.1:46526066-46526085 MS.gene068883:CDS 35.0%
GCAACATCAAAATAACCCAA+AGG - chr2.1:46525986-46526005 None:intergenic 35.0%
TAAAGGAGATGCTATCATGA+TGG + chr2.1:46526402-46526421 MS.gene068883:CDS 35.0%
TAAGATTCCAGTGGTTTCAA+AGG - chr2.1:46527449-46527468 None:intergenic 35.0%
TCAGATTTCCGATCAAGAAT+TGG + chr2.1:46526225-46526244 MS.gene068883:CDS 35.0%
TCATACTCATCCATATCAAC+AGG - chr2.1:46525746-46525765 None:intergenic 35.0%
TCCTGATAATCTTCTATGAC+AGG - chr2.1:46527169-46527188 None:intergenic 35.0%
TCGGCGAATTAACGTAAATA+GGG - chr2.1:46527708-46527727 None:intergenic 35.0%
TCTTCAGCATCTTCTTGAAT+TGG - chr2.1:46526800-46526819 None:intergenic 35.0%
TGATAGAATGGCTAGAGAAA+AGG + chr2.1:46526850-46526869 MS.gene068883:CDS 35.0%
TGATATGGATGAGTATGAGA+AGG + chr2.1:46525748-46525767 MS.gene068883:CDS 35.0%
TGTCATAGAAGATTATCAGG+AGG + chr2.1:46527168-46527187 MS.gene068883:CDS 35.0%
TTCGGCGAATTAACGTAAAT+AGG - chr2.1:46527709-46527728 None:intergenic 35.0%
TTGATAGACCATGTGTGTTA+CGG - chr2.1:46527996-46528015 None:intergenic 35.0%
TTGGATGAAATTGCCAAGTT+GGG + chr2.1:46526244-46526263 MS.gene068883:CDS 35.0%
! ATCTCTTTCCAACTGTTTCT+GGG - chr2.1:46526183-46526202 None:intergenic 35.0%
! CATGATGGAAGCTGAAAATT+TGG + chr2.1:46526417-46526436 MS.gene068883:CDS 35.0%
! TGCATCCTTCAGATTTTTCT+GGG + chr2.1:46526477-46526496 MS.gene068883:CDS 35.0%
! TTGCATCCTTCAGATTTTTC+TGG + chr2.1:46526476-46526495 MS.gene068883:CDS 35.0%
!! TTATTTGCTATCTCCTCAAG+CGG - chr2.1:46527100-46527119 None:intergenic 35.0%
AACTCCTTGATAAGAGCTGA+CGG + chr2.1:46526974-46526993 MS.gene068883:CDS 40.0%
AAGAACTTGAGGGGAAAAGA+AGG + chr2.1:46526053-46526072 MS.gene068883:CDS 40.0%
AAGGACGATAACTATGAACC+TGG + chr2.1:46525650-46525669 MS.gene068883:CDS 40.0%
AAGGTTACATTACTCACGCA+AGG + chr2.1:46528120-46528139 MS.gene068883:CDS 40.0%
ACCCACAATGGTATCACTAA+TGG - chr2.1:46525573-46525592 None:intergenic 40.0%
AGATTCCAGTGGTTTCAAAG+GGG - chr2.1:46527447-46527466 None:intergenic 40.0%
AGCTTTCAGGATTTCATCCT+CGG - chr2.1:46525363-46525382 None:intergenic 40.0%
AGGAGTTCTTAAAGGAGTCA+AGG - chr2.1:46526365-46526384 None:intergenic 40.0%
AGGTTACATTACTCACGCAA+GGG + chr2.1:46528121-46528140 MS.gene068883:CDS 40.0%
ATAGCATCTCCTTTACTAGC+TGG - chr2.1:46526397-46526416 None:intergenic 40.0%
ATCCTCTTCAATCTTCTCCT+CGG - chr2.1:46526825-46526844 None:intergenic 40.0%
ATGAAAAAAAGTCTGCGCTC+TGG + chr2.1:46526725-46526744 MS.gene068883:CDS 40.0%
CCCTGTCATAGAAGATTATC+AGG + chr2.1:46527165-46527184 MS.gene068883:CDS 40.0%
CTCATCCATATCAACAGGAT+CGG - chr2.1:46525741-46525760 None:intergenic 40.0%
CTCCTTTCTTATCATCTCGT+CGG - chr2.1:46527041-46527060 None:intergenic 40.0%
GAATTGGTTGCATCACTATC+TGG - chr2.1:46526784-46526803 None:intergenic 40.0%
GAGCTAGTTGCATATTATGG+TGG + chr2.1:46527528-46527547 MS.gene068883:intron 40.0%
GATCTCTTTCCAACTGTTTC+TGG - chr2.1:46526184-46526203 None:intergenic 40.0%
GCAAATAAGGAGAGAGAGAA+AGG + chr2.1:46527112-46527131 MS.gene068883:CDS 40.0%
GCTTGAGGAGATAGCAAATA+AGG + chr2.1:46527099-46527118 MS.gene068883:CDS 40.0%
GTTATCGTCCTTAACACAAG+TGG - chr2.1:46525642-46525661 None:intergenic 40.0%
TAGAGAAAAGGCAGAAGAAG+AGG + chr2.1:46526862-46526881 MS.gene068883:CDS 40.0%
TCATCCATATCAACAGGATC+GGG - chr2.1:46525740-46525759 None:intergenic 40.0%
TCATTCAAAGGGAACTTCCT+AGG + chr2.1:46526921-46526940 MS.gene068883:CDS 40.0%
TCGTTTGCAAATCTGCAGAA+CGG + chr2.1:46525432-46525451 MS.gene068883:CDS 40.0%
TGAGAAGTAAGCTGGATGAT+GGG + chr2.1:46528070-46528089 MS.gene068883:CDS 40.0%
TGAGAATGTGAGAAGTAAGC+TGG + chr2.1:46528062-46528081 MS.gene068883:CDS 40.0%
TGATAGACCATGTGTGTTAC+GGG - chr2.1:46527995-46528014 None:intergenic 40.0%
TGATGTTGCTGATGAGGATA+GGG + chr2.1:46525997-46526016 MS.gene068883:CDS 40.0%
TGCAGATTTGCAAACGAGTA+GGG - chr2.1:46525429-46525448 None:intergenic 40.0%
TGCTTCAACAAAATCCTCGA+GGG - chr2.1:46527935-46527954 None:intergenic 40.0%
TGGGTGTCTGTGGATAATTA+GGG - chr2.1:46526336-46526355 None:intergenic 40.0%
TGGTTTCAAAGGGGTGAAAA+GGG - chr2.1:46527438-46527457 None:intergenic 40.0%
TTCTTAAAGGAGTCAAGGCT+TGG - chr2.1:46526360-46526379 None:intergenic 40.0%
TTGATGTTGCTGATGAGGAT+AGG + chr2.1:46525996-46526015 MS.gene068883:CDS 40.0%
TTGGGTGTCTGTGGATAATT+AGG - chr2.1:46526337-46526356 None:intergenic 40.0%
TTTGAGAAAAGGCCTCCTTT+GGG + chr2.1:46525968-46525987 MS.gene068883:CDS 40.0%
TTTGATACCCGTAACACACA+TGG + chr2.1:46527985-46528004 MS.gene068883:CDS 40.0%
! AAAGGAGGCCTTTTCTCAAA+CGG - chr2.1:46525968-46525987 None:intergenic 40.0%
! GATGATTCGACTTGAGAAGA+AGG + chr2.1:46528101-46528120 MS.gene068883:CDS 40.0%
! GCAAGGGATGGTTTTTCTTT+TGG + chr2.1:46526602-46526621 MS.gene068883:CDS 40.0%
! GCATCCTTCAGATTTTTCTG+GGG + chr2.1:46526478-46526497 MS.gene068883:CDS 40.0%
! GTTCTCTGAGGAGTTCTTAA+AGG - chr2.1:46526373-46526392 None:intergenic 40.0%
! TCTTTTGGCATGACTACGAA+GGG + chr2.1:46526617-46526636 MS.gene068883:CDS 40.0%
! TGTTCACCTTTTCACCCTTT+GGG + chr2.1:46527291-46527310 MS.gene068883:intron 40.0%
! TTCTTTTGGCATGACTACGA+AGG + chr2.1:46526616-46526635 MS.gene068883:CDS 40.0%
! TTGTTCACCTTTTCACCCTT+TGG + chr2.1:46527290-46527309 MS.gene068883:intron 40.0%
!! ACTCATTTTTAGGCTGTGGA+AGG - chr2.1:46526760-46526779 None:intergenic 40.0%
!! TGGTACTCATTTTTAGGCTG+TGG - chr2.1:46526764-46526783 None:intergenic 40.0%
AAAAGGCAGAAGAAGAGGCA+CGG + chr2.1:46526867-46526886 MS.gene068883:CDS 45.0%
AAGAGAGAATTGAAGGCTGC+TGG + chr2.1:46525884-46525903 MS.gene068883:CDS 45.0%
AAGGATGCAACTCCTAACAG+TGG - chr2.1:46526466-46526485 None:intergenic 45.0%
AATCACGTTGTGTACCCCAA+AGG - chr2.1:46527309-46527328 None:intergenic 45.0%
AATTTCCTAGGGTCATCACC+AGG - chr2.1:46525671-46525690 None:intergenic 45.0%
AGAAACAGCTTGAGGAAGCA+AGG + chr2.1:46525840-46525859 MS.gene068883:CDS 45.0%
AGAGAGAATTGAAGGCTGCT+GGG + chr2.1:46525885-46525904 MS.gene068883:CDS 45.0%
AGCATTGTCATGCTCCAGTA+AGG - chr2.1:46527071-46527090 None:intergenic 45.0%
AGCCGACGAGATGATAAGAA+AGG + chr2.1:46527036-46527055 MS.gene068883:CDS 45.0%
AGGCTGCTGGGATTGATATA+AGG + chr2.1:46525897-46525916 MS.gene068883:CDS 45.0%
AGTATCTTCGTGAGGCAATG+GGG + chr2.1:46527896-46527915 MS.gene068883:CDS 45.0%
ATATGTAGCTCATCCCTGAG+AGG - chr2.1:46526647-46526666 None:intergenic 45.0%
ATCACGTTGTGTACCCCAAA+GGG - chr2.1:46527308-46527327 None:intergenic 45.0%
CAATGCTAAGTATCCGCTTG+AGG + chr2.1:46527084-46527103 MS.gene068883:CDS 45.0%
CAGAGAACACCAGCTAGTAA+AGG + chr2.1:46526385-46526404 MS.gene068883:CDS 45.0%
CAGTATCTTCGTGAGGCAAT+GGG + chr2.1:46527895-46527914 MS.gene068883:CDS 45.0%
CATTGCCTCACGAAGATACT+GGG - chr2.1:46527896-46527915 None:intergenic 45.0%
CTAATAAGCTCCAGAGATGC+TGG - chr2.1:46526953-46526972 None:intergenic 45.0%
CTAATGGTTCTCCACTGAGT+AGG - chr2.1:46525557-46525576 None:intergenic 45.0%
CTGAATGACCCAGAAACAGT+TGG + chr2.1:46526172-46526191 MS.gene068883:CDS 45.0%
CTGCAGATTTGCAAACGAGT+AGG - chr2.1:46525430-46525449 None:intergenic 45.0%
CTGGGATTGATATAAGGCAG+CGG + chr2.1:46525903-46525922 MS.gene068883:CDS 45.0%
GATCCAAACGAGCAGCAAAT+GGG + chr2.1:46527134-46527153 MS.gene068883:CDS 45.0%
GCATGAAAATGATTCCCTCG+AGG + chr2.1:46527918-46527937 MS.gene068883:CDS 45.0%
GGTTTCAAAGGGGTGAAAAG+GGG - chr2.1:46527437-46527456 None:intergenic 45.0%
GTAAAGCTCGTTGGTATGAG+TGG + chr2.1:46525456-46525475 MS.gene068883:CDS 45.0%
GTGACCCCAGAAAAATCTGA+AGG - chr2.1:46526485-46526504 None:intergenic 45.0%
GTGAGAAGTAAGCTGGATGA+TGG + chr2.1:46528069-46528088 MS.gene068883:CDS 45.0%
GTGCTTCAACAAAATCCTCG+AGG - chr2.1:46527936-46527955 None:intergenic 45.0%
GTGGTTTCAAAGGGGTGAAA+AGG - chr2.1:46527439-46527458 None:intergenic 45.0%
GTTTGAGAAAAGGCCTCCTT+TGG + chr2.1:46525967-46525986 MS.gene068883:CDS 45.0%
TCCTCTTCAATCTTCTCCTC+GGG - chr2.1:46526824-46526843 None:intergenic 45.0%
TCTTCTATGACAGGGATAGC+AGG - chr2.1:46527160-46527179 None:intergenic 45.0%
TCTTGATCGGAAATCTGAGG+TGG - chr2.1:46526223-46526242 None:intergenic 45.0%
TGAAAATTTGGCCAGGCTGA+GGG + chr2.1:46526429-46526448 MS.gene068883:CDS 45.0%
TGAGAAAGCAAGACGTTGCA+AGG + chr2.1:46526095-46526114 MS.gene068883:CDS 45.0%
TGGAAGCTGAAAATTTGGCC+AGG + chr2.1:46526422-46526441 MS.gene068883:CDS 45.0%
TGGGGTCACTCCTAAGAAAA+AGG + chr2.1:46526496-46526515 MS.gene068883:intron 45.0%
TTACTAGCTGGTGTTCTCTG+AGG - chr2.1:46526385-46526404 None:intergenic 45.0%
TTCTGGGTCATTCAGCTTCT+TGG - chr2.1:46526167-46526186 None:intergenic 45.0%
! AAAGGAGTCAAGGCTTGGTT+GGG - chr2.1:46526355-46526374 None:intergenic 45.0%
! CATGGTCTATCAAGTGTTGC+TGG + chr2.1:46528003-46528022 MS.gene068883:CDS 45.0%
! GTTCACCTTTTCACCCTTTG+GGG + chr2.1:46527292-46527311 MS.gene068883:intron 45.0%
! TAAAGGAGTCAAGGCTTGGT+TGG - chr2.1:46526356-46526375 None:intergenic 45.0%
!! AAGTAAGCTGGATGATGGGA+AGG + chr2.1:46528074-46528093 MS.gene068883:CDS 45.0%
!! CAGCATCTCTGGAGCTTATT+AGG + chr2.1:46526951-46526970 MS.gene068883:CDS 45.0%
!! TTTCACCCCTTTGAAACCAC+TGG + chr2.1:46527439-46527458 MS.gene068883:intron 45.0%
!! AAAAATTAAATAGAAAAATT+TGG + chr2.1:46527472-46527491 MS.gene068883:intron 5.0%
!! TTATAATCATTTAATTAATT+AGG + chr2.1:46527589-46527608 MS.gene068883:intron 5.0%
AAAGGAGCTTCCAGCCTTAC+TGG + chr2.1:46527054-46527073 MS.gene068883:CDS 50.0%
AACAGCTTGAGGAAGCAAGG+AGG + chr2.1:46525843-46525862 MS.gene068883:CDS 50.0%
ACCCTAGGAAATTGCGTCCA+GGG + chr2.1:46525678-46525697 MS.gene068883:CDS 50.0%
AGCTAGGGAGAAACAGCTTG+AGG + chr2.1:46525832-46525851 MS.gene068883:CDS 50.0%
AGGTGTTTGGACAAACACCG+AGG + chr2.1:46525343-46525362 MS.gene068883:CDS 50.0%
ATAAGCTCCAGAGATGCTGG+AGG - chr2.1:46526950-46526969 None:intergenic 50.0%
CAGAACGGTGTAAAGCTCGT+TGG + chr2.1:46525447-46525466 MS.gene068883:CDS 50.0%
CCAGTATCTTCGTGAGGCAA+TGG + chr2.1:46527894-46527913 MS.gene068883:CDS 50.0%
CCATTGCCTCACGAAGATAC+TGG - chr2.1:46527897-46527916 None:intergenic 50.0%
CCTTAACACAAGTGGCGTCA+AGG - chr2.1:46525634-46525653 None:intergenic 50.0%
CCTTACTCCCAGAAGTGGTA+TGG + chr2.1:46526574-46526593 MS.gene068883:CDS 50.0%
CCTTGACGCCACTTGTGTTA+AGG + chr2.1:46525631-46525650 MS.gene068883:CDS 50.0%
CTAAGCTTACGCCTACTCAG+TGG + chr2.1:46525543-46525562 MS.gene068883:CDS 50.0%
CTCCTTCGCAGTGTCTTGAA+CGG + chr2.1:46525597-46525616 MS.gene068883:CDS 50.0%
CTGAAAATTTGGCCAGGCTG+AGG + chr2.1:46526428-46526447 MS.gene068883:CDS 50.0%
GACTGATTGGACTCGAGAAG+AGG + chr2.1:46525499-46525518 MS.gene068883:CDS 50.0%
GAGTCTCAGACACCACTGTT+AGG + chr2.1:46526451-46526470 MS.gene068883:CDS 50.0%
GCAGGCTTCGATTCAGGATT+CGG - chr2.1:46525713-46525732 None:intergenic 50.0%
GGATCCAAACGAGCAGCAAA+TGG + chr2.1:46527133-46527152 MS.gene068883:CDS 50.0%
GGATTGATATAAGGCAGCGG+AGG + chr2.1:46525906-46525925 MS.gene068883:CDS 50.0%
GGTTATGCCAGTGACCTTGT+GGG + chr2.1:46526265-46526284 MS.gene068883:CDS 50.0%
GTCACTGGCATAACCCAACT+TGG - chr2.1:46526260-46526279 None:intergenic 50.0%
GTCTCCGTCAGCTCTTATCA+AGG - chr2.1:46526981-46527000 None:intergenic 50.0%
GTGTACCCCAAAGGGTGAAA+AGG - chr2.1:46527300-46527319 None:intergenic 50.0%
TACCGTTCAAGACACTGCGA+AGG - chr2.1:46525602-46525621 None:intergenic 50.0%
TACGAAGGGAACTCCTCTCA+GGG + chr2.1:46526631-46526650 MS.gene068883:CDS 50.0%
TGAAGCCCAGTATCTTCGTG+AGG + chr2.1:46527888-46527907 MS.gene068883:CDS 50.0%
TGAGTTCATTGCTGCCCACA+AGG - chr2.1:46526282-46526301 None:intergenic 50.0%
TGCTTTCCGAAGCATGTGCT+CGG + chr2.1:46525774-46525793 MS.gene068883:CDS 50.0%
TGTTGCTGATGAGGATAGGG+AGG + chr2.1:46526000-46526019 MS.gene068883:CDS 50.0%
TTGATATAAGGCAGCGGAGG+AGG + chr2.1:46525909-46525928 MS.gene068883:CDS 50.0%
TTGGCATGCAGAATAGCAGC+CGG - chr2.1:46526148-46526167 None:intergenic 50.0%
TTGTCATGCTCCAGTAAGGC+TGG - chr2.1:46527067-46527086 None:intergenic 50.0%
! ATGAACCTGGTGATGACCCT+AGG + chr2.1:46525663-46525682 MS.gene068883:CDS 50.0%
! CCATACCACTTCTGGGAGTA+AGG - chr2.1:46526577-46526596 None:intergenic 50.0%
! TGATCGGAAATCTGAGGTGG+TGG - chr2.1:46526220-46526239 None:intergenic 50.0%
!! ATCTCGTCGGCTTGCTCAAT+AGG - chr2.1:46527028-46527047 None:intergenic 50.0%
!! TCTCGTCGGCTTGCTCAATA+GGG - chr2.1:46527027-46527046 None:intergenic 50.0%
ACGAGTAGCACTGCTGCCTT+CGG - chr2.1:46526305-46526324 None:intergenic 55.0%
AGAAGATGCTGAAGAGCCCG+AGG + chr2.1:46526805-46526824 MS.gene068883:CDS 55.0%
AGCCAACCGAGCACATGCTT+CGG - chr2.1:46525783-46525802 None:intergenic 55.0%
AGCGGAGGAGGAAAAGGAGA+GGG + chr2.1:46525921-46525940 MS.gene068883:CDS 55.0%
AGTAAGGCCTGCACTTCCAG+GGG - chr2.1:46526561-46526580 None:intergenic 55.0%
ATTGCTGCCCACAAGGTCAC+TGG - chr2.1:46526275-46526294 None:intergenic 55.0%
CCCCTGGACGCAATTTCCTA+GGG - chr2.1:46525682-46525701 None:intergenic 55.0%
CCCTAGGAAATTGCGTCCAG+GGG + chr2.1:46525679-46525698 MS.gene068883:CDS 55.0%
CTACGAAGGGAACTCCTCTC+AGG + chr2.1:46526630-46526649 MS.gene068883:CDS 55.0%
GACCCTAGGAAATTGCGTCC+AGG + chr2.1:46525677-46525696 MS.gene068883:CDS 55.0%
GAGTAAGGCCTGCACTTCCA+GGG - chr2.1:46526562-46526581 None:intergenic 55.0%
GCCCGAGGAGAAGATTGAAG+AGG + chr2.1:46526820-46526839 MS.gene068883:CDS 55.0%
GCGACCCGATCCTGTTGATA+TGG + chr2.1:46525733-46525752 MS.gene068883:CDS 55.0%
GCTTGCTCAATAGGGGTTGG+TGG - chr2.1:46527019-46527038 None:intergenic 55.0%
GGATTCGGATCAATCTCCCC+TGG - chr2.1:46525698-46525717 None:intergenic 55.0%
GGCAGCAATGAACTCACCGA+AGG + chr2.1:46526286-46526305 MS.gene068883:CDS 55.0%
GGGTTATGCCAGTGACCTTG+TGG + chr2.1:46526264-46526283 MS.gene068883:CDS 55.0%
TAAGGCAGCGGAGGAGGAAA+AGG + chr2.1:46525915-46525934 MS.gene068883:CDS 55.0%
TCCCCTGGACGCAATTTCCT+AGG - chr2.1:46525683-46525702 None:intergenic 55.0%
TGCTGATGAGGATAGGGAGG+TGG + chr2.1:46526003-46526022 MS.gene068883:CDS 55.0%
TGGCATGCAGAATAGCAGCC+GGG - chr2.1:46526147-46526166 None:intergenic 55.0%
TTCCGAAGCATGTGCTCGGT+TGG + chr2.1:46525778-46525797 MS.gene068883:CDS 55.0%
TTGCAGAGGGAGTCAGCAGT+GGG - chr2.1:46526538-46526557 None:intergenic 55.0%
! AAGGCTTGGTTGGGTGTCTG+TGG - chr2.1:46526346-46526365 None:intergenic 55.0%
! AGAAGAAGAGGCACGGCAGA+AGG + chr2.1:46526874-46526893 MS.gene068883:CDS 55.0%
! AGGCCCATTTGCTGCTCGTT+TGG - chr2.1:46527140-46527159 None:intergenic 55.0%
! CGAAGGAGTACGACCCACAA+TGG - chr2.1:46525585-46525604 None:intergenic 55.0%
! GCTCGGTTGGCTAACACGAA+AGG + chr2.1:46525791-46525810 MS.gene068883:CDS 55.0%
! TCGGCTTGCTCAATAGGGGT+TGG - chr2.1:46527022-46527041 None:intergenic 55.0%
!! AAGTGGTATGGCGCTGGCAA+GGG + chr2.1:46526586-46526605 MS.gene068883:CDS 55.0%
!! CTCGTCGGCTTGCTCAATAG+GGG - chr2.1:46527026-46527045 None:intergenic 55.0%
AGAGATGCTGGAGGAGGCCT+AGG - chr2.1:46526941-46526960 None:intergenic 60.0%
AGCTCCAGAGATGCTGGAGG+AGG - chr2.1:46526947-46526966 None:intergenic 60.0%
CAGCGGAGGAGGAAAAGGAG+AGG + chr2.1:46525920-46525939 MS.gene068883:CDS 60.0%
GAGGGAGTCAGCAGTGGGAT+TGG - chr2.1:46526533-46526552 None:intergenic 60.0%
GCACTTCCAGGGGTTGCAGA+GGG - chr2.1:46526551-46526570 None:intergenic 60.0%
GCAGGCCTTACTCCCAGAAG+TGG + chr2.1:46526569-46526588 MS.gene068883:intron 60.0%
GCCAGCGCCATACCACTTCT+GGG - chr2.1:46526584-46526603 None:intergenic 60.0%
GGAGTAAGGCCTGCACTTCC+AGG - chr2.1:46526563-46526582 None:intergenic 60.0%
GGCATGCAGAATAGCAGCCG+GGG - chr2.1:46526146-46526165 None:intergenic 60.0%
GTTGCAGAGGGAGTCAGCAG+TGG - chr2.1:46526539-46526558 None:intergenic 60.0%
TAGGCCTCCTCCAGCATCTC+TGG + chr2.1:46526940-46526959 MS.gene068883:CDS 60.0%
TCCCAGAAGTGGTATGGCGC+TGG + chr2.1:46526580-46526599 MS.gene068883:CDS 60.0%
TGCACTTCCAGGGGTTGCAG+AGG - chr2.1:46526552-46526571 None:intergenic 60.0%
TGCAGAGAGGCAAGATGCCC+CGG + chr2.1:46526126-46526145 MS.gene068883:CDS 60.0%
TGCCAGCGCCATACCACTTC+TGG - chr2.1:46526585-46526604 None:intergenic 60.0%
! TCTGCAACCCCTGGAAGTGC+AGG + chr2.1:46526551-46526570 MS.gene068883:intron 60.0%
! TGTCTGAGACTCCCTCAGCC+TGG - chr2.1:46526443-46526462 None:intergenic 60.0%
!! GAAGTGGTATGGCGCTGGCA+AGG + chr2.1:46526585-46526604 MS.gene068883:CDS 60.0%
CTGACTCCCTCTGCAACCCC+TGG + chr2.1:46526542-46526561 MS.gene068883:intron 65.0%
GGTCGCGCAGGCTTCGATTC+AGG - chr2.1:46525719-46525738 None:intergenic 65.0%
TCAACAGGATCGGGTCGCGC+AGG - chr2.1:46525731-46525750 None:intergenic 65.0%
!! GGTATGGCGCTGGCAAGGGA+TGG + chr2.1:46526590-46526609 MS.gene068883:CDS 65.0%


Chromosome Type Strat End Strand Name
chr2.1 gene 46525335 46528164 46525335 ID=MS.gene068883
chr2.1 mRNA 46525335 46528164 46525335 ID=MS.gene068883.t1;Parent=MS.gene068883
chr2.1 exon 46525335 46526499 46525335 ID=MS.gene068883.t1.exon1;Parent=MS.gene068883.t1
chr2.1 CDS 46525335 46526499 46525335 ID=cds.MS.gene068883.t1;Parent=MS.gene068883.t1
chr2.1 exon 46526573 46527204 46526573 ID=MS.gene068883.t1.exon2;Parent=MS.gene068883.t1
chr2.1 CDS 46526573 46527204 46526573 ID=cds.MS.gene068883.t1;Parent=MS.gene068883.t1
chr2.1 exon 46527868 46528164 46527868 ID=MS.gene068883.t1.exon3;Parent=MS.gene068883.t1
chr2.1 CDS 46527868 46528164 46527868 ID=cds.MS.gene068883.t1;Parent=MS.gene068883.t1
Gene Sequence

>MS.gene068883

ATGAGCATAGGTGTTTGGACAAACACCGAGGATGAAATCCTGAAAGCTGCTGTTATGAAATATGGTAAAAATCAGTGGGCTCATATCTCGTCCCTACTCGTTTGCAAATCTGCAGAACGGTGTAAAGCTCGTTGGTATGAGTGGTTAGATCCTTCCATTAAGAAGACTGATTGGACTCGAGAAGAGGATGAGAAGCTACTTCATCTTGCTAAGCTTACGCCTACTCAGTGGAGAACCATTAGTGATACCATTGTGGGTCGTACTCCTTCGCAGTGTCTTGAACGGTATGAGAAACTCCTTGACGCCACTTGTGTTAAGGACGATAACTATGAACCTGGTGATGACCCTAGGAAATTGCGTCCAGGGGAGATTGATCCGAATCCTGAATCGAAGCCTGCGCGACCCGATCCTGTTGATATGGATGAGTATGAGAAGGAGATGCTTTCCGAAGCATGTGCTCGGTTGGCTAACACGAAAGGTAAAAAAGCAAAAAGGAAAGCTAGGGAGAAACAGCTTGAGGAAGCAAGGAGGCTTGCTTCTTTACAGAAAAAGAGAGAATTGAAGGCTGCTGGGATTGATATAAGGCAGCGGAGGAGGAAAAGGAGAGGGATTGATTATAATGCAGAAATTCCGTTTGAGAAAAGGCCTCCTTTGGGTTATTTTGATGTTGCTGATGAGGATAGGGAGGTGGAGCAGCATGCATTCCCTACTACTATAGAAGAACTTGAGGGGAAAAGAAGGATTGATGTTGAGGCTCAGTTGAGAAAGCAAGACGTTGCAAGGAATAAAATTGCAGAGAGGCAAGATGCCCCGGCTGCTATTCTGCATGCCAAGAAGCTGAATGACCCAGAAACAGTTGGAAAGAGATCAAAACTGATGCTTCCACCACCTCAGATTTCCGATCAAGAATTGGATGAAATTGCCAAGTTGGGTTATGCCAGTGACCTTGTGGGCAGCAATGAACTCACCGAAGGCAGCAGTGCTACTCGTGCTCTTCTCCCTAATTATCCACAGACACCCAACCAAGCCTTGACTCCTTTAAGAACTCCTCAGAGAACACCAGCTAGTAAAGGAGATGCTATCATGATGGAAGCTGAAAATTTGGCCAGGCTGAGGGAGTCTCAGACACCACTGTTAGGAGTTGCATCCTTCAGATTTTTCTGGGGTCACTCCTAAGAAAAAGGAAATTCACATTCCAATCCCACTGCTGACTCCCTCTGCAACCCCTGGAAGTGCAGGCCTTACTCCCAGAAGTGGTATGGCGCTGGCAAGGGATGGTTTTTCTTTTGGCATGACTACGAAGGGAACTCCTCTCAGGGATGAGCTACATATCAATGAAGATATGGAGATGCGTGATAGTGCTAGACTCGAGCTACAGAGACAAGCTGATATGAAAAAAAGTCTGCGCTCTGGTTTAAGCAGCCTTCCACAGCCTAAAAATGAGTACCAGATAGTGATGCAACCAATTCAAGAAGATGCTGAAGAGCCCGAGGAGAAGATTGAAGAGGATATGTCTGATAGAATGGCTAGAGAAAAGGCAGAAGAAGAGGCACGGCAGAAGGCGTTACTTAGAAAACGATCAAAAGTCATTCAAAGGGAACTTCCTAGGCCTCCTCCAGCATCTCTGGAGCTTATTAGGAACTCCTTGATAAGAGCTGACGGAGACAAAAGTTCATTTGTTCCACCAACCCCTATTGAGCAAGCCGACGAGATGATAAGAAAGGAGCTTCCAGCCTTACTGGAGCATGACAATGCTAAGTATCCGCTTGAGGAGATAGCAAATAAGGAGAGAGAGAAAGGATCCAAACGAGCAGCAAATGGGCCTGCTATCCCTGTCATAGAAGATTATCAGGAGGACGAGATGAAATCTGTATGTTTTTACTAATTTATCTGCTCACCTTTAATTTTATTTTTATGCCTTTTACTTCGCTTTTCTTCATTTGCTACTAAGTAGCTTGTTCACCTTTTCACCCTTTGGGGTACACAACGTGATTTTTCATTTAGATGATACACATGCTTTTTCTGATGATCTTGATCTTGAAATTATTAGTACTTTGTCCGATTATTAATTACTTGGCATCATCATTTTTATGCTGAAACCCCTTTTCACCCCTTTGAAACCACTGGAATCTTATGTAAAAATTAAATAGAAAAATTTGGACATCTTTTTAATTTATGTATCATCTCCTTTATGAGCTAGTTGCATATTATGGTGGTATGTAATATGTATTGTATCGTCCTTGTTTTTTTTCAGTTATAATCATTTAATTAATTAGGTTGAAGTTAGTTTTAGTTTCTCGCAATTGTTGTTCTCTATTTCTTTCGTTTTTGTAACAAGTGATTCTTCAATTGCCTTTTACAACATTTATTCCCTATTTACGTTAATTCGCCGAATGATAGTAATACAAACTTCCAAACTGTTTGTTAATTATTATTTGACTCCTTAAATTTGACTATGGAGATGAGCTTGTACGTTGATGAATGATAATATCTGTGTTGACTGTTATTTATTTTTATCTGTTGTTCTCAATAGGCTGATCAGTTGATAAAAGATGAAGCCCAGTATCTTCGTGAGGCAATGGGGCATGAAAATGATTCCCTCGAGGATTTTGTTGAAGCACATACAACATGCATTAATGATTTAATGTACTTTGATACCCGTAACACACATGGTCTATCAAGTGTTGCTGGAAACGCAGAGAAGCTAGCAGCTTTGCAGAATGAATTTGAGAATGTGAGAAGTAAGCTGGATGATGGGAAGGAGAAGATGATTCGACTTGAGAAGAAGGTTACATTACTCACGCAAGGGTACGAGTTATGCTACTGCTAA
Protein sequence

>MS.gene068883.t1

MSIGVWTNTEDEILKAAVMKYGKNQWAHISSLLVCKSAERCKARWYEWLDPSIKKTDWTREEDEKLLHLAKLTPTQWRTISDTIVGRTPSQCLERYEKLLDATCVKDDNYEPGDDPRKLRPGEIDPNPESKPARPDPVDMDEYEKEMLSEACARLANTKGKKAKRKAREKQLEEARRLASLQKKRELKAAGIDIRQRRRKRRGIDYNAEIPFEKRPPLGYFDVADEDREVEQHAFPTTIEELEGKRRIDVEAQLRKQDVARNKIAERQDAPAAILHAKKLNDPETVGKRSKLMLPPPQISDQELDEIAKLGYASDLVGSNELTEGSSATRALLPNYPQTPNQALTPLRTPQRTPASKGDAIMMEAENLARLRESQTPLLGVASFRFFWGLTPRSGMALARDGFSFGMTTKGTPLRDELHINEDMEMRDSARLELQRQADMKKSLRSGLSSLPQPKNEYQIVMQPIQEDAEEPEEKIEEDMSDRMAREKAEEEARQKALLRKRSKVIQRELPRPPPASLELIRNSLIRADGDKSSFVPPTPIEQADEMIRKELPALLEHDNAKYPLEEIANKEREKGSKRAANGPAIPVIEDYQEDEMKSADQLIKDEAQYLREAMGHENDSLEDFVEAHTTCINDLMYFDTRNTHGLSSVAGNAEKLAALQNEFENVRSKLDDGKEKMIRLEKKVTLLTQGYELCYC