AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene052998

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene052998.t1 MTR_7g115550 89.722 720 44 2 4 694 8 726 0.0 1226
MS.gene052998.t1 MTR_7g115550 89.765 723 44 2 4 697 8 729 0.0 1226
MS.gene052998.t1 MTR_5g010470 82.030 729 52 6 1 696 1 683 0.0 1080
MS.gene052998.t1 MTR_7g061330 38.095 105 64 1 4 107 62 166 6.19e-15 78.6
MS.gene052998.t1 MTR_7g061330 31.008 129 78 2 4 132 114 231 4.66e-11 66.2
MS.gene052998.t1 MTR_7g461410 38.095 105 64 1 4 107 62 166 6.19e-15 78.6
MS.gene052998.t1 MTR_7g461410 31.008 129 78 2 4 132 114 231 4.66e-11 66.2
MS.gene052998.t1 MTR_7g061330 38.095 105 64 1 4 107 26 130 7.48e-15 78.2
MS.gene052998.t1 MTR_7g061330 31.008 129 78 2 4 132 78 195 5.17e-11 66.2
MS.gene052998.t1 MTR_8g468380 34.021 97 64 0 3 99 22 118 9.14e-13 71.2
MS.gene052998.t1 MTR_3g110028 34.286 105 68 1 4 107 34 138 9.80e-13 72.0
MS.gene052998.t1 MTR_3g110028 36.471 85 54 0 4 88 86 170 2.87e-12 70.9
MS.gene052998.t1 MTR_1g026870 34.343 99 64 1 4 101 34 132 1.86e-12 71.2
MS.gene052998.t1 MTR_5g038910 33.945 109 68 1 3 111 26 130 1.97e-12 70.5
MS.gene052998.t1 MTR_5g038910 33.945 109 68 1 3 111 26 130 2.54e-12 69.7
MS.gene052998.t1 MTR_7g451170 37.647 85 53 0 4 88 192 276 5.33e-12 68.9
MS.gene052998.t1 MTR_7g035350 33.981 103 65 1 5 104 227 329 1.20e-11 67.8
MS.gene052998.t1 MTR_4g088015 35.294 85 55 0 4 88 222 306 1.34e-11 67.8
MS.gene052998.t1 MTR_7g037260 36.471 85 54 0 4 88 146 230 2.66e-11 66.2
MS.gene052998.t1 MTR_6g027340 34.783 92 60 0 6 97 19 110 3.05e-11 63.9
MS.gene052998.t1 MTR_4g105660 37.647 85 53 0 4 88 181 265 3.66e-11 66.2
MS.gene052998.t1 MTR_6g027360 35.052 97 63 0 1 97 76 172 5.07e-11 64.7
MS.gene052998.t1 MTR_5g010650 37.349 83 52 0 6 88 113 195 5.76e-11 65.9
MS.gene052998.t1 MTR_1g057980 32.258 93 63 0 4 96 81 173 6.91e-11 64.3
MS.gene052998.t1 MTR_6g027370 31.959 97 66 0 4 100 100 196 6.94e-11 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene052998.t1 AT1G09770 71.468 722 171 5 4 693 8 726 0.0 967
MS.gene052998.t1 AT5G02320 37.143 105 65 1 4 107 16 120 1.60e-14 77.0
MS.gene052998.t1 AT5G02320 34.118 85 56 0 4 88 68 152 8.97e-11 65.5
MS.gene052998.t1 AT5G02320 37.143 105 65 1 4 107 76 180 1.62e-14 77.4
MS.gene052998.t1 AT5G02320 34.118 85 56 0 4 88 128 212 7.06e-11 65.9
MS.gene052998.t1 AT5G02320 37.143 105 65 1 4 107 76 180 1.62e-14 77.4
MS.gene052998.t1 AT5G02320 34.118 85 56 0 4 88 128 212 7.06e-11 65.9
MS.gene052998.t1 AT3G18100 34.375 96 63 0 4 99 547 642 7.11e-14 75.9
MS.gene052998.t1 AT3G18100 36.082 97 60 2 6 101 498 593 2.67e-11 67.4
MS.gene052998.t1 AT3G18100 34.375 96 63 0 4 99 499 594 1.21e-13 75.1
MS.gene052998.t1 AT3G18100 36.082 97 60 2 6 101 450 545 4.65e-11 66.6
MS.gene052998.t1 AT3G09370 36.190 105 66 1 4 107 84 188 1.24e-13 74.3
MS.gene052998.t1 AT3G09370 36.190 105 66 1 4 107 79 183 1.25e-13 74.3
MS.gene052998.t1 AT3G09370 36.190 105 66 1 4 107 92 196 1.46e-13 74.3
MS.gene052998.t1 AT3G18100 34.375 96 63 0 4 99 386 481 1.51e-13 74.7
MS.gene052998.t1 AT3G18100 36.082 97 60 2 6 101 337 432 6.07e-11 66.2
MS.gene052998.t1 AT5G11510 37.374 99 61 1 4 101 30 128 1.56e-13 74.7
MS.gene052998.t1 AT5G11510 37.374 99 61 1 4 101 30 128 1.56e-13 74.7
MS.gene052998.t1 AT5G11510 37.374 99 61 1 4 101 30 128 1.56e-13 74.7
MS.gene052998.t1 AT3G18100 34.375 96 63 0 4 99 334 429 1.85e-13 74.3
MS.gene052998.t1 AT3G18100 36.082 97 60 2 6 101 285 380 7.80e-11 65.9
MS.gene052998.t1 AT5G11510 37.374 99 61 1 4 101 30 128 2.72e-13 73.9
MS.gene052998.t1 AT5G11510 37.374 99 61 1 4 101 30 128 2.93e-13 73.9
MS.gene052998.t1 AT4G18770 38.824 85 52 0 4 88 218 302 8.74e-13 71.2
MS.gene052998.t1 AT4G32730 35.354 99 63 1 4 101 36 134 1.58e-12 71.6
MS.gene052998.t1 AT4G32730 35.354 99 63 1 4 101 36 134 1.58e-12 71.6
MS.gene052998.t1 AT4G32730 35.354 99 63 1 4 101 36 134 1.58e-12 71.6
MS.gene052998.t1 AT4G32730 35.354 99 63 1 4 101 36 134 1.58e-12 71.6
MS.gene052998.t1 AT4G32730 35.354 99 63 1 4 101 36 134 2.51e-12 70.9
MS.gene052998.t1 AT4G33450 32.990 97 65 0 4 100 20 116 4.06e-12 67.4
MS.gene052998.t1 AT2G02820 30.579 121 70 1 3 109 30 150 1.08e-11 68.2
MS.gene052998.t1 AT4G00540 37.500 112 61 2 4 106 47 158 1.13e-11 67.8
MS.gene052998.t1 AT4G00540 37.500 112 61 2 4 106 45 156 1.33e-11 67.8
MS.gene052998.t1 AT2G02820 30.579 121 70 1 3 109 30 150 1.60e-11 67.4
MS.gene052998.t1 AT2G02820 30.579 121 70 1 3 109 30 150 2.34e-11 66.2
MS.gene052998.t1 AT5G40360 35.294 85 55 0 4 88 159 243 2.52e-11 66.2
MS.gene052998.t1 AT1G14350 31.959 97 66 0 3 99 25 121 3.20e-11 66.6
MS.gene052998.t1 AT1G14350 31.959 97 66 0 3 99 25 121 3.20e-11 66.6
MS.gene052998.t1 AT1G17950 31.313 99 68 0 2 100 4 102 3.35e-11 64.7
MS.gene052998.t1 AT5G17800 34.343 99 65 0 2 100 92 190 3.80e-11 65.5
MS.gene052998.t1 AT4G38620 31.655 139 84 5 4 138 15 146 5.73e-11 64.3

Find 196 sgRNAs with CRISPR-Local

Find 257 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GATCTCTTTCCAACTGTTTC+TGG 0.013419 2.1:-46809553 None:intergenic
TTCTGGGTCATTCAGCTTCT+TGG 0.153986 2.1:-46809536 None:intergenic
TGCCAGCGCCATACCACTTC+TGG 0.197747 2.1:-46809954 None:intergenic
ATTTCATAACAGCAGCTTTC+AGG 0.225007 2.1:-46808745 None:intergenic
CAGCATCTCTGGAGCTTATT+AGG 0.228626 2.1:+46810323 MS.gene052998:CDS
TTGGGTGTCTGTGGATAATT+AGG 0.241982 2.1:-46809706 None:intergenic
CATGATGGAAGCTGAAAATT+TGG 0.248037 2.1:+46809789 MS.gene052998:CDS
AAAGGAGCTTCCAGCCTTAC+TGG 0.273790 2.1:+46810426 MS.gene052998:CDS
GTTCTCTGAGGAGTTCTTAA+AGG 0.283026 2.1:-46809742 None:intergenic
AAAGCTGCTGTTATGAAATA+TGG 0.287568 2.1:+46808749 MS.gene052998:CDS
GAATTGGTTGCATCACTATC+TGG 0.302904 2.1:-46810153 None:intergenic
GGTCGCGCAGGCTTCGATTC+AGG 0.310060 2.1:-46809088 None:intergenic
AGTCCACTCAGTCTTCTTAA+TGG 0.314486 2.1:-46808861 None:intergenic
GTTTGAGAAAAGGCCTCCTT+TGG 0.314999 2.1:+46809339 MS.gene052998:CDS
CCCTGTCATAGAAGATTATC+AGG 0.315605 2.1:+46810537 MS.gene052998:CDS
CCATTGCCTCACGAAGATAC+TGG 0.317289 2.1:-46811266 None:intergenic
ATCTCTTTCCAACTGTTTCT+GGG 0.330928 2.1:-46809552 None:intergenic
TCTTCAGCATCTTCTTGAAT+TGG 0.331097 2.1:-46810169 None:intergenic
ATCTCGTCGGCTTGCTCAAT+AGG 0.342238 2.1:-46810397 None:intergenic
TCAAGTTCTTCTATAGTAGT+AGG 0.344179 2.1:-46809412 None:intergenic
TAGGCCTCCTCCAGCATCTC+TGG 0.367513 2.1:+46810312 MS.gene052998:CDS
CAGAAATTCCGTTTGAGAAA+AGG 0.368252 2.1:+46809329 MS.gene052998:CDS
GCTTGAGGAGATAGCAAATA+AGG 0.373696 2.1:+46810471 MS.gene052998:CDS
CCTTGACGCCACTTGTGTTA+AGG 0.386161 2.1:+46809003 MS.gene052998:CDS
TCTCGTCGGCTTGCTCAATA+GGG 0.387795 2.1:-46810396 None:intergenic
AGGCCCATTTGCTGCTCGTT+TGG 0.388358 2.1:-46810509 None:intergenic
TCAGATTTCCGATCAAGAAT+TGG 0.389053 2.1:+46809597 MS.gene052998:CDS
GCAGGCTTCGATTCAGGATT+CGG 0.393602 2.1:-46809082 None:intergenic
CCAAAATGAGCATAGGTGTT+TGG 0.397192 2.1:+46808702 None:intergenic
CTACGAAGGGAACTCCTCTC+AGG 0.399722 2.1:+46810002 MS.gene052998:CDS
AGGCTGCTGGGATTGATATA+AGG 0.409355 2.1:+46809269 MS.gene052998:CDS
AGAGATGCTGGAGGAGGCCT+AGG 0.415805 2.1:-46810310 None:intergenic
CATGGTCTATCAAGTGTTGC+TGG 0.417267 2.1:+46811375 MS.gene052998:CDS
GGATCCAAACGAGCAGCAAA+TGG 0.417377 2.1:+46810505 MS.gene052998:CDS
TTGATAGACCATGTGTGTTA+CGG 0.418408 2.1:-46811365 None:intergenic
GCTACATATCAATGAAGATA+TGG 0.420115 2.1:+46810030 MS.gene052998:CDS
TAAGGCAGCGGAGGAGGAAA+AGG 0.424744 2.1:+46809287 MS.gene052998:CDS
TGATAGACCATGTGTGTTAC+GGG 0.426495 2.1:-46811364 None:intergenic
TTGGATGAAATTGCCAAGTT+GGG 0.429250 2.1:+46809616 MS.gene052998:CDS
AGCTTTCAGGATTTCATCCT+CGG 0.436489 2.1:-46808732 None:intergenic
CAGTATCTTCGTGAGGCAAT+GGG 0.437430 2.1:+46811267 MS.gene052998:CDS
ATTGGATGAAATTGCCAAGT+TGG 0.444138 2.1:+46809615 MS.gene052998:CDS
AAGAACTTGAGGGGAAAAGA+AGG 0.444403 2.1:+46809425 MS.gene052998:CDS
GCCAGCGCCATACCACTTCT+GGG 0.445237 2.1:-46809953 None:intergenic
GCAAATAAGGAGAGAGAGAA+AGG 0.446065 2.1:+46810484 MS.gene052998:CDS
CTAATAAGCTCCAGAGATGC+TGG 0.447893 2.1:-46810322 None:intergenic
ATCCTCTTCAATCTTCTCCT+CGG 0.449369 2.1:-46810194 None:intergenic
CCATACCACTTCTGGGAGTA+AGG 0.450702 2.1:-46809946 None:intergenic
AAGAGAGAATTGAAGGCTGC+TGG 0.451049 2.1:+46809256 MS.gene052998:CDS
TCCTGATAATCTTCTATGAC+AGG 0.451422 2.1:-46810538 None:intergenic
TCATACTCATCCATATCAAC+AGG 0.454291 2.1:-46809115 None:intergenic
ACCCACAATGGTATCACTAA+TGG 0.455710 2.1:-46808942 None:intergenic
AGAAGAAGAGGCACGGCAGA+AGG 0.457675 2.1:+46810246 MS.gene052998:CDS
TCCCCTGGACGCAATTTCCT+AGG 0.458724 2.1:-46809052 None:intergenic
AAAGGAGTCAAGGCTTGGTT+GGG 0.461730 2.1:-46809724 None:intergenic
TGCAGAGAGGCAAGATGCCC+CGG 0.470266 2.1:+46809498 MS.gene052998:CDS
GAGTCTCAGACACCACTGTT+AGG 0.471291 2.1:+46809823 MS.gene052998:CDS
CTGAAAATTTGGCCAGGCTG+AGG 0.471566 2.1:+46809800 MS.gene052998:CDS
TTCTTAAAGGAGTCAAGGCT+TGG 0.471836 2.1:-46809729 None:intergenic
TGGCATGCAGAATAGCAGCC+GGG 0.473211 2.1:-46809516 None:intergenic
TTGATATAAGGCAGCGGAGG+AGG 0.474103 2.1:+46809281 MS.gene052998:CDS
AGCCAACCGAGCACATGCTT+CGG 0.476372 2.1:-46809152 None:intergenic
TGATGTTGCTGATGAGGATA+GGG 0.479449 2.1:+46809369 MS.gene052998:CDS
AATTTCATCCAATTCTTGAT+CGG 0.479560 2.1:-46809605 None:intergenic
GCGACCCGATCCTGTTGATA+TGG 0.481942 2.1:+46809105 MS.gene052998:CDS
CCAGTATCTTCGTGAGGCAA+TGG 0.484875 2.1:+46811266 MS.gene052998:CDS
TGTCTGAGACTCCCTCAGCC+TGG 0.484905 2.1:-46809812 None:intergenic
AACGATCAAAAGTCATTCAA+AGG 0.485643 2.1:+46810281 MS.gene052998:CDS
TCCTCTTCAATCTTCTCCTC+GGG 0.488427 2.1:-46810193 None:intergenic
CTCCTTTCTTATCATCTCGT+CGG 0.493278 2.1:-46810410 None:intergenic
TCATTCAAAGGGAACTTCCT+AGG 0.493709 2.1:+46810293 MS.gene052998:CDS
ACGAGTAGCACTGCTGCCTT+CGG 0.495690 2.1:-46809674 None:intergenic
TTCCGAAGCATGTGCTCGGT+TGG 0.496711 2.1:+46809150 MS.gene052998:CDS
TCTTCTATGACAGGGATAGC+AGG 0.496725 2.1:-46810529 None:intergenic
GTCTCCGTCAGCTCTTATCA+AGG 0.499862 2.1:-46810350 None:intergenic
TTGTCATGCTCCAGTAAGGC+TGG 0.499885 2.1:-46810436 None:intergenic
TGCTTTCCGAAGCATGTGCT+CGG 0.502006 2.1:+46809146 MS.gene052998:CDS
GTGAGAAGTAAGCTGGATGA+TGG 0.502979 2.1:+46811441 MS.gene052998:CDS
CTCATCCATATCAACAGGAT+CGG 0.503902 2.1:-46809110 None:intergenic
AAGTAAGCTGGATGATGGGA+AGG 0.506469 2.1:+46811446 MS.gene052998:CDS
ATAGCATCTCCTTTACTAGC+TGG 0.506651 2.1:-46809766 None:intergenic
AGAGGATATGTCTGATAGAA+TGG 0.507303 2.1:+46810210 MS.gene052998:CDS
AGCTCCAGAGATGCTGGAGG+AGG 0.508047 2.1:-46810316 None:intergenic
ACCATTGTCATGCTCCAGTA+AGG 0.510371 2.1:-46810440 None:intergenic
GATGATTCGACTTGAGAAGA+AGG 0.511403 2.1:+46811473 MS.gene052998:CDS
TGATAGAATGGCTAGAGAAA+AGG 0.511992 2.1:+46810222 MS.gene052998:CDS
ATTGCTGCCCACAAGGTCAC+TGG 0.512710 2.1:-46809644 None:intergenic
TTGGCATGCAGAATAGCAGC+CGG 0.514383 2.1:-46809517 None:intergenic
TTTGAGAAAAGGCCTCCTTT+GGG 0.515050 2.1:+46809340 MS.gene052998:CDS
CAGAGAACACCAGCTAGTAA+AGG 0.515217 2.1:+46809757 MS.gene052998:CDS
TGCAGATTTGCAAACGAGTA+GGG 0.518174 2.1:-46808798 None:intergenic
CATTGCCTCACGAAGATACT+GGG 0.519621 2.1:-46811265 None:intergenic
TGGGTGTCTGTGGATAATTA+GGG 0.519886 2.1:-46809705 None:intergenic
TTGATGTTGCTGATGAGGAT+AGG 0.524642 2.1:+46809368 MS.gene052998:CDS
TCGGCTTGCTCAATAGGGGT+TGG 0.524733 2.1:-46810391 None:intergenic
TGAAAATTTGGCCAGGCTGA+GGG 0.525084 2.1:+46809801 MS.gene052998:CDS
GACCCTAGGAAATTGCGTCC+AGG 0.525314 2.1:+46809049 MS.gene052998:CDS
CTGCAGATTTGCAAACGAGT+AGG 0.526600 2.1:-46808799 None:intergenic
ACCATTAGTGATACCATTGT+GGG 0.527710 2.1:+46808941 MS.gene052998:CDS
CAAGTTCTTCTATAGTAGTA+GGG 0.528361 2.1:-46809411 None:intergenic
TAAAGGAGTCAAGGCTTGGT+TGG 0.528607 2.1:-46809725 None:intergenic
GAAGTGGTATGGCGCTGGCA+AGG 0.528663 2.1:+46809957 MS.gene052998:CDS
TGAGAATGTGAGAAGTAAGC+TGG 0.529343 2.1:+46811434 MS.gene052998:CDS
GCTTGCTCAATAGGGGTTGG+TGG 0.531592 2.1:-46810388 None:intergenic
CACTCAGTCTTCTTAATGGA+AGG 0.532258 2.1:-46808857 None:intergenic
TCATCCATATCAACAGGATC+GGG 0.534614 2.1:-46809109 None:intergenic
ACCCTAGGAAATTGCGTCCA+GGG 0.536757 2.1:+46809050 MS.gene052998:CDS
AAGTGGTATGGCGCTGGCAA+GGG 0.537683 2.1:+46809958 MS.gene052998:CDS
TCCCAGAAGTGGTATGGCGC+TGG 0.538783 2.1:+46809952 MS.gene052998:CDS
GATCCAAACGAGCAGCAAAT+GGG 0.542547 2.1:+46810506 MS.gene052998:CDS
AGAGAGAATTGAAGGCTGCT+GGG 0.549517 2.1:+46809257 MS.gene052998:CDS
TGATCGGAAATCTGAGGTGG+TGG 0.551298 2.1:-46809589 None:intergenic
CCTTACTCCCAGAAGTGGTA+TGG 0.551739 2.1:+46809946 MS.gene052998:CDS
AGCCGACGAGATGATAAGAA+AGG 0.552168 2.1:+46810408 MS.gene052998:CDS
GAAAAGAAGGATTGATGTTG+AGG 0.552508 2.1:+46809438 MS.gene052998:CDS
GGTTATGCCAGTGACCTTGT+GGG 0.556103 2.1:+46809637 MS.gene052998:CDS
TGAGAAAGCAAGACGTTGCA+AGG 0.556741 2.1:+46809467 MS.gene052998:CDS
GGGTTATGCCAGTGACCTTG+TGG 0.560009 2.1:+46809636 MS.gene052998:CDS
GGATTCGGATCAATCTCCCC+TGG 0.561665 2.1:-46809067 None:intergenic
CCCCTGGACGCAATTTCCTA+GGG 0.562986 2.1:-46809051 None:intergenic
AGAAACAGCTTGAGGAAGCA+AGG 0.565360 2.1:+46809212 MS.gene052998:CDS
TCAACAGGATCGGGTCGCGC+AGG 0.566355 2.1:-46809100 None:intergenic
ACGATCAAAAGTCATTCAAA+GGG 0.567785 2.1:+46810282 MS.gene052998:CDS
AGGAGTTCTTAAAGGAGTCA+AGG 0.570143 2.1:-46809734 None:intergenic
ACTACTATAGAAGAACTTGA+GGG 0.571318 2.1:+46809415 MS.gene052998:CDS
TAAAGGAGATGCTATCATGA+TGG 0.573963 2.1:+46809774 MS.gene052998:CDS
CTGAATGACCCAGAAACAGT+TGG 0.574442 2.1:+46809544 MS.gene052998:CDS
TTACTAGCTGGTGTTCTCTG+AGG 0.578802 2.1:-46809754 None:intergenic
TAGAGAAAAGGCAGAAGAAG+AGG 0.579560 2.1:+46810234 MS.gene052998:CDS
ATGAACCTGGTGATGACCCT+AGG 0.580871 2.1:+46809035 MS.gene052998:CDS
TTATTTGCTATCTCCTCAAG+CGG 0.583374 2.1:-46810469 None:intergenic
GTCACTGGCATAACCCAACT+TGG 0.585958 2.1:-46809629 None:intergenic
AAGGTTACATTACTCACGCA+AGG 0.586053 2.1:+46811492 MS.gene052998:CDS
GTAAAGCTCGTTGGTATGAG+TGG 0.588526 2.1:+46808828 MS.gene052998:CDS
AACTCCTTGATAAGAGCTGA+CGG 0.589304 2.1:+46810346 MS.gene052998:CDS
AAGGCTTGGTTGGGTGTCTG+TGG 0.589342 2.1:-46809715 None:intergenic
CCTGATAATCTTCTATGACA+GGG 0.589597 2.1:-46810537 None:intergenic
GACTGAGTGGACTCGAGAAG+AGG 0.592125 2.1:+46808871 MS.gene052998:CDS
TGCTTCAACAAAATCCTCGA+GGG 0.592155 2.1:-46811304 None:intergenic
CCTTAACACAAGTGGCGTCA+AGG 0.592424 2.1:-46809003 None:intergenic
GGTATGGCGCTGGCAAGGGA+TGG 0.593619 2.1:+46809962 MS.gene052998:CDS
CTCCTTCGCAGTGTCTTGAA+CGG 0.594673 2.1:+46808969 MS.gene052998:CDS
TGCTGATGAGGATAGGGAGG+TGG 0.595277 2.1:+46809375 MS.gene052998:CDS
CAGAACGGTGTAAAGCTCGT+TGG 0.595953 2.1:+46808819 MS.gene052998:CDS
AGTGTTGCCAAAATGAGCAT+AGG 0.596521 2.1:+46808695 None:intergenic
AACCATTAGTGATACCATTG+TGG 0.597390 2.1:+46808940 MS.gene052998:CDS
TGAGAAGTAAGCTGGATGAT+GGG 0.598085 2.1:+46811442 MS.gene052998:CDS
TACTACTATAGAAGAACTTG+AGG 0.598439 2.1:+46809414 MS.gene052998:CDS
AAAAGGCAGAAGAAGAGGCA+CGG 0.599691 2.1:+46810239 MS.gene052998:CDS
TGAGTTCATTGCTGCCCACA+AGG 0.602663 2.1:-46809651 None:intergenic
CTAATGGTTCTCCACTGAGT+AGG 0.603402 2.1:-46808926 None:intergenic
TGGAAGCTGAAAATTTGGCC+AGG 0.607474 2.1:+46809794 MS.gene052998:CDS
GCTCGGTTGGCTAACACGAA+AGG 0.608258 2.1:+46809163 MS.gene052998:CDS
AGCGGAGGAGGAAAAGGAGA+GGG 0.609580 2.1:+46809293 MS.gene052998:CDS
TACGAAGGGAACTCCTCTCA+GGG 0.614373 2.1:+46810003 MS.gene052998:CDS
CAATGGTAAGTATCCGCTTG+AGG 0.616439 2.1:+46810456 MS.gene052998:CDS
AATTTCCTAGGGTCATCACC+AGG 0.619740 2.1:-46809040 None:intergenic
CAGCGGAGGAGGAAAAGGAG+AGG 0.619802 2.1:+46809292 MS.gene052998:CDS
CTGGGATTGATATAAGGCAG+CGG 0.621106 2.1:+46809275 MS.gene052998:CDS
GTTATCGTCCTTAACACAAG+TGG 0.622208 2.1:-46809011 None:intergenic
GCAGGCCTTACTCCCAGAAG+TGG 0.624004 2.1:+46809941 MS.gene052998:intron
CGAAGGAGTACGACCCACAA+TGG 0.624367 2.1:-46808954 None:intergenic
CCCTAGGAAATTGCGTCCAG+GGG 0.631019 2.1:+46809051 MS.gene052998:CDS
CTCGTCGGCTTGCTCAATAG+GGG 0.633774 2.1:-46810395 None:intergenic
GCCTTACTGGAGCATGACAA+TGG 0.634325 2.1:+46810439 MS.gene052998:CDS
TTTGATACCCGTAACACACA+TGG 0.638610 2.1:+46811357 MS.gene052998:CDS
GGCATGCAGAATAGCAGCCG+GGG 0.639810 2.1:-46809515 None:intergenic
AAGGACGATAACTATGAACC+TGG 0.641325 2.1:+46809022 MS.gene052998:CDS
TGTTGCTGATGAGGATAGGG+AGG 0.645550 2.1:+46809372 MS.gene052998:CDS
GCAACATCAAAATAACCCAA+AGG 0.645993 2.1:-46809355 None:intergenic
AGAAGATGCTGAAGAGCCCG+AGG 0.646686 2.1:+46810177 MS.gene052998:CDS
CTACTATAGAAGAACTTGAG+GGG 0.654103 2.1:+46809416 MS.gene052998:CDS
GCCCGAGGAGAAGATTGAAG+AGG 0.654306 2.1:+46810192 MS.gene052998:CDS
AATTCTTGATCGGAAATCTG+AGG 0.665249 2.1:-46809595 None:intergenic
AGCTAGGGAGAAACAGCTTG+AGG 0.665565 2.1:+46809204 MS.gene052998:CDS
TACCGTTCAAGACACTGCGA+AGG 0.666095 2.1:-46808971 None:intergenic
AGGTTACATTACTCACGCAA+GGG 0.667391 2.1:+46811493 MS.gene052998:CDS
GGCAGCAATGAACTCACCGA+AGG 0.667861 2.1:+46809658 MS.gene052998:CDS
CTAAGCTTACGCCTACTCAG+TGG 0.671805 2.1:+46808915 MS.gene052998:CDS
TCGTTTGCAAATCTGCAGAA+CGG 0.675444 2.1:+46808804 MS.gene052998:CDS
TCTTGATCGGAAATCTGAGG+TGG 0.678038 2.1:-46809592 None:intergenic
ACATCAAAATAACCCAAAGG+AGG 0.679868 2.1:-46809352 None:intergenic
CTTCCATTAAGAAGACTGAG+TGG 0.680364 2.1:+46808858 MS.gene052998:CDS
TGAAGCCCAGTATCTTCGTG+AGG 0.688109 2.1:+46811260 MS.gene052998:CDS
GTGCTTCAACAAAATCCTCG+AGG 0.688213 2.1:-46811305 None:intergenic
ATATGTAGCTCATCCCTGAG+AGG 0.688581 2.1:-46810016 None:intergenic
AGTATCTTCGTGAGGCAATG+GGG 0.691770 2.1:+46811268 MS.gene052998:CDS
GGATTGATATAAGGCAGCGG+AGG 0.699913 2.1:+46809278 MS.gene052998:CDS
ATAAGCTCCAGAGATGCTGG+AGG 0.701115 2.1:-46810319 None:intergenic
AACAGCTTGAGGAAGCAAGG+AGG 0.704973 2.1:+46809215 MS.gene052998:CDS
AAGGATGCAACTCCTAACAG+TGG 0.713627 2.1:-46809835 None:intergenic
TGATATGGATGAGTATGAGA+AGG 0.714958 2.1:+46809120 MS.gene052998:CDS
TGTCATAGAAGATTATCAGG+AGG 0.727087 2.1:+46810540 MS.gene052998:CDS
GCATGAAAATGATTCCCTCG+AGG 0.731393 2.1:+46811290 MS.gene052998:CDS
CAAGGAATAAAATTGCAGAG+AGG 0.745082 2.1:+46809485 MS.gene052998:CDS
AGGTGTTTGGACAAACACCG+AGG 0.789261 2.1:+46808715 MS.gene052998:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AGGCATAAAAATAAAATTAA+AGG - chr2.1:46810607-46810626 None:intergenic 15.0%
!! ATTATAACTGAAAAAAAACA+AGG - chr2.1:46810948-46810967 None:intergenic 15.0%
!! AAGTTTGTATTACTATCATT+CGG - chr2.1:46811099-46811118 None:intergenic 20.0%
!! ATAATAATTAACAAGCAGTT+TGG - chr2.1:46811122-46811141 None:intergenic 20.0%
! AATTTCATCCAATTCTTGAT+CGG - chr2.1:46809608-46809627 None:intergenic 25.0%
! GAAATATGGTAAAAATCAGT+GGG + chr2.1:46808763-46808782 MS.gene052998:CDS 25.0%
! GATGCCAAGTAATTAATAAT+CGG - chr2.1:46810768-46810787 None:intergenic 25.0%
! TAGGGAATAAATGTTGTAAA+AGG - chr2.1:46811062-46811081 None:intergenic 25.0%
! TGAAATATGGTAAAAATCAG+TGG + chr2.1:46808762-46808781 MS.gene052998:CDS 25.0%
! TTGTCCGATTATTAATTACT+TGG + chr2.1:46810761-46810780 MS.gene052998:intron 25.0%
!! ATGTGAATTTCCTTTTTCTT+AGG - chr2.1:46809881-46809900 None:intergenic 25.0%
!! ATTTTTACATAAGATTCCAG+TGG - chr2.1:46810830-46810849 None:intergenic 25.0%
!! TTTTATCTGTTGTTCTCAAT+AGG + chr2.1:46811218-46811237 MS.gene052998:intron 25.0%
AAAAAGCAAAAAGGAAAGCT+AGG + chr2.1:46809188-46809207 MS.gene052998:CDS 30.0%
AAAAGCAAAAAGGAAAGCTA+GGG + chr2.1:46809189-46809208 MS.gene052998:CDS 30.0%
AAAGCTGCTGTTATGAAATA+TGG + chr2.1:46808749-46808768 MS.gene052998:CDS 30.0%
AACGATCAAAAGTCATTCAA+AGG + chr2.1:46810281-46810300 MS.gene052998:CDS 30.0%
AATATGCAACTAGCTCATAA+AGG - chr2.1:46810896-46810915 None:intergenic 30.0%
ACAGAAAAAGAGAGAATTGA+AGG + chr2.1:46809249-46809268 MS.gene052998:CDS 30.0%
ACGATCAAAAGTCATTCAAA+GGG + chr2.1:46810282-46810301 MS.gene052998:CDS 30.0%
ACTACTATAGAAGAACTTGA+GGG + chr2.1:46809415-46809434 MS.gene052998:CDS 30.0%
ATGAAGAAAAGCGAAGTAAA+AGG - chr2.1:46810627-46810646 None:intergenic 30.0%
CAAGTTCTTCTATAGTAGTA+GGG - chr2.1:46809414-46809433 None:intergenic 30.0%
CATCTCCATAGTCAAATTTA+AGG - chr2.1:46811151-46811170 None:intergenic 30.0%
CGAAAGGTAAAAAAGCAAAA+AGG + chr2.1:46809179-46809198 MS.gene052998:CDS 30.0%
GCTACATATCAATGAAGATA+TGG + chr2.1:46810030-46810049 MS.gene052998:CDS 30.0%
TACTACTATAGAAGAACTTG+AGG + chr2.1:46809414-46809433 MS.gene052998:CDS 30.0%
TATGAGCTAGTTGCATATTA+TGG + chr2.1:46810897-46810916 MS.gene052998:intron 30.0%
TCAAGTTCTTCTATAGTAGT+AGG - chr2.1:46809415-46809434 None:intergenic 30.0%
TGACTCCTTAAATTTGACTA+TGG + chr2.1:46811143-46811162 MS.gene052998:intron 30.0%
!! ACTATCTGGTACTCATTTTT+AGG - chr2.1:46810142-46810161 None:intergenic 30.0%
!!! TTATTTTGATGTTGCTGATG+AGG + chr2.1:46809363-46809382 MS.gene052998:CDS 30.0%
AACCATTAGTGATACCATTG+TGG + chr2.1:46808940-46808959 MS.gene052998:CDS 35.0%
AAGATTCCAGTGGTTTCAAA+GGG - chr2.1:46810820-46810839 None:intergenic 35.0%
AATTCTTGATCGGAAATCTG+AGG - chr2.1:46809598-46809617 None:intergenic 35.0%
ACATCAAAATAACCCAAAGG+AGG - chr2.1:46809355-46809374 None:intergenic 35.0%
ACCATTAGTGATACCATTGT+GGG + chr2.1:46808941-46808960 MS.gene052998:CDS 35.0%
AGAGGATATGTCTGATAGAA+TGG + chr2.1:46810210-46810229 MS.gene052998:CDS 35.0%
ATTGGATGAAATTGCCAAGT+TGG + chr2.1:46809615-46809634 MS.gene052998:CDS 35.0%
ATTTCATAACAGCAGCTTTC+AGG - chr2.1:46808748-46808767 None:intergenic 35.0%
CAAGGAATAAAATTGCAGAG+AGG + chr2.1:46809485-46809504 MS.gene052998:CDS 35.0%
CAGAAATTCCGTTTGAGAAA+AGG + chr2.1:46809329-46809348 MS.gene052998:CDS 35.0%
CCTGATAATCTTCTATGACA+GGG - chr2.1:46810540-46810559 None:intergenic 35.0%
CTACTATAGAAGAACTTGAG+GGG + chr2.1:46809416-46809435 MS.gene052998:CDS 35.0%
GAAAAGAAGGATTGATGTTG+AGG + chr2.1:46809438-46809457 MS.gene052998:CDS 35.0%
GCAACATCAAAATAACCCAA+AGG - chr2.1:46809358-46809377 None:intergenic 35.0%
TAAAGGAGATGCTATCATGA+TGG + chr2.1:46809774-46809793 MS.gene052998:CDS 35.0%
TAAGATTCCAGTGGTTTCAA+AGG - chr2.1:46810821-46810840 None:intergenic 35.0%
TCAGATTTCCGATCAAGAAT+TGG + chr2.1:46809597-46809616 MS.gene052998:CDS 35.0%
TCATACTCATCCATATCAAC+AGG - chr2.1:46809118-46809137 None:intergenic 35.0%
TCCTGATAATCTTCTATGAC+AGG - chr2.1:46810541-46810560 None:intergenic 35.0%
TCGGCGAATTAACGTAAATA+GGG - chr2.1:46811080-46811099 None:intergenic 35.0%
TCTTCAGCATCTTCTTGAAT+TGG - chr2.1:46810172-46810191 None:intergenic 35.0%
TGATAGAATGGCTAGAGAAA+AGG + chr2.1:46810222-46810241 MS.gene052998:CDS 35.0%
TGATATGGATGAGTATGAGA+AGG + chr2.1:46809120-46809139 MS.gene052998:CDS 35.0%
TGTCATAGAAGATTATCAGG+AGG + chr2.1:46810540-46810559 MS.gene052998:CDS 35.0%
TTCGGCGAATTAACGTAAAT+AGG - chr2.1:46811081-46811100 None:intergenic 35.0%
TTGATAGACCATGTGTGTTA+CGG - chr2.1:46811368-46811387 None:intergenic 35.0%
TTGGATGAAATTGCCAAGTT+GGG + chr2.1:46809616-46809635 MS.gene052998:CDS 35.0%
! ATCTCTTTCCAACTGTTTCT+GGG - chr2.1:46809555-46809574 None:intergenic 35.0%
! CATGATGGAAGCTGAAAATT+TGG + chr2.1:46809789-46809808 MS.gene052998:CDS 35.0%
! TGCATCCTTCAGATTTTTCT+GGG + chr2.1:46809849-46809868 MS.gene052998:CDS 35.0%
! TTGCATCCTTCAGATTTTTC+TGG + chr2.1:46809848-46809867 MS.gene052998:CDS 35.0%
!! TTATTTGCTATCTCCTCAAG+CGG - chr2.1:46810472-46810491 None:intergenic 35.0%
AACTCCTTGATAAGAGCTGA+CGG + chr2.1:46810346-46810365 MS.gene052998:CDS 40.0%
AAGAACTTGAGGGGAAAAGA+AGG + chr2.1:46809425-46809444 MS.gene052998:CDS 40.0%
AAGGACGATAACTATGAACC+TGG + chr2.1:46809022-46809041 MS.gene052998:CDS 40.0%
AAGGTTACATTACTCACGCA+AGG + chr2.1:46811492-46811511 MS.gene052998:CDS 40.0%
ACCCACAATGGTATCACTAA+TGG - chr2.1:46808945-46808964 None:intergenic 40.0%
AGATTCCAGTGGTTTCAAAG+GGG - chr2.1:46810819-46810838 None:intergenic 40.0%
AGCTTTCAGGATTTCATCCT+CGG - chr2.1:46808735-46808754 None:intergenic 40.0%
AGGAGTTCTTAAAGGAGTCA+AGG - chr2.1:46809737-46809756 None:intergenic 40.0%
AGGTTACATTACTCACGCAA+GGG + chr2.1:46811493-46811512 MS.gene052998:CDS 40.0%
AGTCCACTCAGTCTTCTTAA+TGG - chr2.1:46808864-46808883 None:intergenic 40.0%
ATAGCATCTCCTTTACTAGC+TGG - chr2.1:46809769-46809788 None:intergenic 40.0%
ATCCTCTTCAATCTTCTCCT+CGG - chr2.1:46810197-46810216 None:intergenic 40.0%
ATGAAAAAAAGTCTGCGCTC+TGG + chr2.1:46810097-46810116 MS.gene052998:CDS 40.0%
CACTCAGTCTTCTTAATGGA+AGG - chr2.1:46808860-46808879 None:intergenic 40.0%
CCCTGTCATAGAAGATTATC+AGG + chr2.1:46810537-46810556 MS.gene052998:CDS 40.0%
CTCATCCATATCAACAGGAT+CGG - chr2.1:46809113-46809132 None:intergenic 40.0%
CTCCTTTCTTATCATCTCGT+CGG - chr2.1:46810413-46810432 None:intergenic 40.0%
CTTCCATTAAGAAGACTGAG+TGG + chr2.1:46808858-46808877 MS.gene052998:CDS 40.0%
GAATTGGTTGCATCACTATC+TGG - chr2.1:46810156-46810175 None:intergenic 40.0%
GAGCTAGTTGCATATTATGG+TGG + chr2.1:46810900-46810919 MS.gene052998:intron 40.0%
GATCTCTTTCCAACTGTTTC+TGG - chr2.1:46809556-46809575 None:intergenic 40.0%
GCAAATAAGGAGAGAGAGAA+AGG + chr2.1:46810484-46810503 MS.gene052998:CDS 40.0%
GCTTGAGGAGATAGCAAATA+AGG + chr2.1:46810471-46810490 MS.gene052998:CDS 40.0%
GTTATCGTCCTTAACACAAG+TGG - chr2.1:46809014-46809033 None:intergenic 40.0%
TAGAGAAAAGGCAGAAGAAG+AGG + chr2.1:46810234-46810253 MS.gene052998:CDS 40.0%
TCATCCATATCAACAGGATC+GGG - chr2.1:46809112-46809131 None:intergenic 40.0%
TCATTCAAAGGGAACTTCCT+AGG + chr2.1:46810293-46810312 MS.gene052998:CDS 40.0%
TCGTTTGCAAATCTGCAGAA+CGG + chr2.1:46808804-46808823 MS.gene052998:CDS 40.0%
TGAGAAGTAAGCTGGATGAT+GGG + chr2.1:46811442-46811461 MS.gene052998:CDS 40.0%
TGAGAATGTGAGAAGTAAGC+TGG + chr2.1:46811434-46811453 MS.gene052998:CDS 40.0%
TGATAGACCATGTGTGTTAC+GGG - chr2.1:46811367-46811386 None:intergenic 40.0%
TGATGTTGCTGATGAGGATA+GGG + chr2.1:46809369-46809388 MS.gene052998:CDS 40.0%
TGCAGATTTGCAAACGAGTA+GGG - chr2.1:46808801-46808820 None:intergenic 40.0%
TGCTTCAACAAAATCCTCGA+GGG - chr2.1:46811307-46811326 None:intergenic 40.0%
TGGGTGTCTGTGGATAATTA+GGG - chr2.1:46809708-46809727 None:intergenic 40.0%
TGGTTTCAAAGGGGTGAAAA+GGG - chr2.1:46810810-46810829 None:intergenic 40.0%
TTCTTAAAGGAGTCAAGGCT+TGG - chr2.1:46809732-46809751 None:intergenic 40.0%
TTGATGTTGCTGATGAGGAT+AGG + chr2.1:46809368-46809387 MS.gene052998:CDS 40.0%
TTGGGTGTCTGTGGATAATT+AGG - chr2.1:46809709-46809728 None:intergenic 40.0%
TTTGAGAAAAGGCCTCCTTT+GGG + chr2.1:46809340-46809359 MS.gene052998:CDS 40.0%
TTTGATACCCGTAACACACA+TGG + chr2.1:46811357-46811376 MS.gene052998:CDS 40.0%
! AAAGGAGGCCTTTTCTCAAA+CGG - chr2.1:46809340-46809359 None:intergenic 40.0%
! GATGATTCGACTTGAGAAGA+AGG + chr2.1:46811473-46811492 MS.gene052998:CDS 40.0%
! GCAAGGGATGGTTTTTCTTT+TGG + chr2.1:46809974-46809993 MS.gene052998:CDS 40.0%
! GCATCCTTCAGATTTTTCTG+GGG + chr2.1:46809850-46809869 MS.gene052998:CDS 40.0%
! GTTCTCTGAGGAGTTCTTAA+AGG - chr2.1:46809745-46809764 None:intergenic 40.0%
! TCTTTTGGCATGACTACGAA+GGG + chr2.1:46809989-46810008 MS.gene052998:CDS 40.0%
! TGTTCACCTTTTCACCCTTT+GGG + chr2.1:46810663-46810682 MS.gene052998:intron 40.0%
! TTCTTTTGGCATGACTACGA+AGG + chr2.1:46809988-46810007 MS.gene052998:CDS 40.0%
! TTGTTCACCTTTTCACCCTT+TGG + chr2.1:46810662-46810681 MS.gene052998:intron 40.0%
!! ACTCATTTTTAGGCTGTGGA+AGG - chr2.1:46810132-46810151 None:intergenic 40.0%
!! TGGTACTCATTTTTAGGCTG+TGG - chr2.1:46810136-46810155 None:intergenic 40.0%
AAAAGGCAGAAGAAGAGGCA+CGG + chr2.1:46810239-46810258 MS.gene052998:CDS 45.0%
AAGAGAGAATTGAAGGCTGC+TGG + chr2.1:46809256-46809275 MS.gene052998:CDS 45.0%
AAGGATGCAACTCCTAACAG+TGG - chr2.1:46809838-46809857 None:intergenic 45.0%
AATCACGTTGTGTACCCCAA+AGG - chr2.1:46810681-46810700 None:intergenic 45.0%
AATTTCCTAGGGTCATCACC+AGG - chr2.1:46809043-46809062 None:intergenic 45.0%
ACCATTGTCATGCTCCAGTA+AGG - chr2.1:46810443-46810462 None:intergenic 45.0%
AGAAACAGCTTGAGGAAGCA+AGG + chr2.1:46809212-46809231 MS.gene052998:CDS 45.0%
AGAGAGAATTGAAGGCTGCT+GGG + chr2.1:46809257-46809276 MS.gene052998:CDS 45.0%
AGCCGACGAGATGATAAGAA+AGG + chr2.1:46810408-46810427 MS.gene052998:CDS 45.0%
AGGCTGCTGGGATTGATATA+AGG + chr2.1:46809269-46809288 MS.gene052998:CDS 45.0%
AGTATCTTCGTGAGGCAATG+GGG + chr2.1:46811268-46811287 MS.gene052998:CDS 45.0%
ATATGTAGCTCATCCCTGAG+AGG - chr2.1:46810019-46810038 None:intergenic 45.0%
ATCACGTTGTGTACCCCAAA+GGG - chr2.1:46810680-46810699 None:intergenic 45.0%
CAATGGTAAGTATCCGCTTG+AGG + chr2.1:46810456-46810475 MS.gene052998:CDS 45.0%
CAGAGAACACCAGCTAGTAA+AGG + chr2.1:46809757-46809776 MS.gene052998:CDS 45.0%
CAGTATCTTCGTGAGGCAAT+GGG + chr2.1:46811267-46811286 MS.gene052998:CDS 45.0%
CATTGCCTCACGAAGATACT+GGG - chr2.1:46811268-46811287 None:intergenic 45.0%
CTAATAAGCTCCAGAGATGC+TGG - chr2.1:46810325-46810344 None:intergenic 45.0%
CTAATGGTTCTCCACTGAGT+AGG - chr2.1:46808929-46808948 None:intergenic 45.0%
CTGAATGACCCAGAAACAGT+TGG + chr2.1:46809544-46809563 MS.gene052998:CDS 45.0%
CTGCAGATTTGCAAACGAGT+AGG - chr2.1:46808802-46808821 None:intergenic 45.0%
CTGGGATTGATATAAGGCAG+CGG + chr2.1:46809275-46809294 MS.gene052998:CDS 45.0%
GATCCAAACGAGCAGCAAAT+GGG + chr2.1:46810506-46810525 MS.gene052998:CDS 45.0%
GCATGAAAATGATTCCCTCG+AGG + chr2.1:46811290-46811309 MS.gene052998:CDS 45.0%
GGTTTCAAAGGGGTGAAAAG+GGG - chr2.1:46810809-46810828 None:intergenic 45.0%
GTAAAGCTCGTTGGTATGAG+TGG + chr2.1:46808828-46808847 MS.gene052998:CDS 45.0%
GTGACCCCAGAAAAATCTGA+AGG - chr2.1:46809857-46809876 None:intergenic 45.0%
GTGAGAAGTAAGCTGGATGA+TGG + chr2.1:46811441-46811460 MS.gene052998:CDS 45.0%
GTGCTTCAACAAAATCCTCG+AGG - chr2.1:46811308-46811327 None:intergenic 45.0%
GTGGTTTCAAAGGGGTGAAA+AGG - chr2.1:46810811-46810830 None:intergenic 45.0%
GTTTGAGAAAAGGCCTCCTT+TGG + chr2.1:46809339-46809358 MS.gene052998:CDS 45.0%
TCCTCTTCAATCTTCTCCTC+GGG - chr2.1:46810196-46810215 None:intergenic 45.0%
TCTTCTATGACAGGGATAGC+AGG - chr2.1:46810532-46810551 None:intergenic 45.0%
TCTTGATCGGAAATCTGAGG+TGG - chr2.1:46809595-46809614 None:intergenic 45.0%
TGAAAATTTGGCCAGGCTGA+GGG + chr2.1:46809801-46809820 MS.gene052998:CDS 45.0%
TGAGAAAGCAAGACGTTGCA+AGG + chr2.1:46809467-46809486 MS.gene052998:CDS 45.0%
TGGAAGCTGAAAATTTGGCC+AGG + chr2.1:46809794-46809813 MS.gene052998:CDS 45.0%
TGGGGTCACTCCTAAGAAAA+AGG + chr2.1:46809868-46809887 MS.gene052998:intron 45.0%
TTACTAGCTGGTGTTCTCTG+AGG - chr2.1:46809757-46809776 None:intergenic 45.0%
TTCTGGGTCATTCAGCTTCT+TGG - chr2.1:46809539-46809558 None:intergenic 45.0%
! AAAGGAGTCAAGGCTTGGTT+GGG - chr2.1:46809727-46809746 None:intergenic 45.0%
! CATGGTCTATCAAGTGTTGC+TGG + chr2.1:46811375-46811394 MS.gene052998:CDS 45.0%
! GTTCACCTTTTCACCCTTTG+GGG + chr2.1:46810664-46810683 MS.gene052998:intron 45.0%
! TAAAGGAGTCAAGGCTTGGT+TGG - chr2.1:46809728-46809747 None:intergenic 45.0%
!! AAGTAAGCTGGATGATGGGA+AGG + chr2.1:46811446-46811465 MS.gene052998:CDS 45.0%
!! CAGCATCTCTGGAGCTTATT+AGG + chr2.1:46810323-46810342 MS.gene052998:CDS 45.0%
!! TTTCACCCCTTTGAAACCAC+TGG + chr2.1:46810811-46810830 MS.gene052998:intron 45.0%
!! AAAAATTAAATAGAAAAATT+TGG + chr2.1:46810844-46810863 MS.gene052998:intron 5.0%
!! TTATAATCATTTAATTAATT+AGG + chr2.1:46810961-46810980 MS.gene052998:intron 5.0%
AAAGGAGCTTCCAGCCTTAC+TGG + chr2.1:46810426-46810445 MS.gene052998:CDS 50.0%
AACAGCTTGAGGAAGCAAGG+AGG + chr2.1:46809215-46809234 MS.gene052998:CDS 50.0%
ACCCTAGGAAATTGCGTCCA+GGG + chr2.1:46809050-46809069 MS.gene052998:CDS 50.0%
AGCTAGGGAGAAACAGCTTG+AGG + chr2.1:46809204-46809223 MS.gene052998:CDS 50.0%
AGGTGTTTGGACAAACACCG+AGG + chr2.1:46808715-46808734 MS.gene052998:CDS 50.0%
ATAAGCTCCAGAGATGCTGG+AGG - chr2.1:46810322-46810341 None:intergenic 50.0%
CAGAACGGTGTAAAGCTCGT+TGG + chr2.1:46808819-46808838 MS.gene052998:CDS 50.0%
CCAGTATCTTCGTGAGGCAA+TGG + chr2.1:46811266-46811285 MS.gene052998:CDS 50.0%
CCATTGCCTCACGAAGATAC+TGG - chr2.1:46811269-46811288 None:intergenic 50.0%
CCTTAACACAAGTGGCGTCA+AGG - chr2.1:46809006-46809025 None:intergenic 50.0%
CCTTACTCCCAGAAGTGGTA+TGG + chr2.1:46809946-46809965 MS.gene052998:CDS 50.0%
CCTTGACGCCACTTGTGTTA+AGG + chr2.1:46809003-46809022 MS.gene052998:CDS 50.0%
CTAAGCTTACGCCTACTCAG+TGG + chr2.1:46808915-46808934 MS.gene052998:CDS 50.0%
CTCCTTCGCAGTGTCTTGAA+CGG + chr2.1:46808969-46808988 MS.gene052998:CDS 50.0%
CTGAAAATTTGGCCAGGCTG+AGG + chr2.1:46809800-46809819 MS.gene052998:CDS 50.0%
GAGTCTCAGACACCACTGTT+AGG + chr2.1:46809823-46809842 MS.gene052998:CDS 50.0%
GCAGGCTTCGATTCAGGATT+CGG - chr2.1:46809085-46809104 None:intergenic 50.0%
GCCTTACTGGAGCATGACAA+TGG + chr2.1:46810439-46810458 MS.gene052998:CDS 50.0%
GGATCCAAACGAGCAGCAAA+TGG + chr2.1:46810505-46810524 MS.gene052998:CDS 50.0%
GGATTGATATAAGGCAGCGG+AGG + chr2.1:46809278-46809297 MS.gene052998:CDS 50.0%
GGTTATGCCAGTGACCTTGT+GGG + chr2.1:46809637-46809656 MS.gene052998:CDS 50.0%
GTCACTGGCATAACCCAACT+TGG - chr2.1:46809632-46809651 None:intergenic 50.0%
GTCTCCGTCAGCTCTTATCA+AGG - chr2.1:46810353-46810372 None:intergenic 50.0%
GTGTACCCCAAAGGGTGAAA+AGG - chr2.1:46810672-46810691 None:intergenic 50.0%
TACCGTTCAAGACACTGCGA+AGG - chr2.1:46808974-46808993 None:intergenic 50.0%
TACGAAGGGAACTCCTCTCA+GGG + chr2.1:46810003-46810022 MS.gene052998:CDS 50.0%
TGAAGCCCAGTATCTTCGTG+AGG + chr2.1:46811260-46811279 MS.gene052998:CDS 50.0%
TGAGTTCATTGCTGCCCACA+AGG - chr2.1:46809654-46809673 None:intergenic 50.0%
TGCTTTCCGAAGCATGTGCT+CGG + chr2.1:46809146-46809165 MS.gene052998:CDS 50.0%
TGTTGCTGATGAGGATAGGG+AGG + chr2.1:46809372-46809391 MS.gene052998:CDS 50.0%
TTGATATAAGGCAGCGGAGG+AGG + chr2.1:46809281-46809300 MS.gene052998:CDS 50.0%
TTGGCATGCAGAATAGCAGC+CGG - chr2.1:46809520-46809539 None:intergenic 50.0%
TTGTCATGCTCCAGTAAGGC+TGG - chr2.1:46810439-46810458 None:intergenic 50.0%
! ATGAACCTGGTGATGACCCT+AGG + chr2.1:46809035-46809054 MS.gene052998:CDS 50.0%
! CCATACCACTTCTGGGAGTA+AGG - chr2.1:46809949-46809968 None:intergenic 50.0%
! TGATCGGAAATCTGAGGTGG+TGG - chr2.1:46809592-46809611 None:intergenic 50.0%
!! ATCTCGTCGGCTTGCTCAAT+AGG - chr2.1:46810400-46810419 None:intergenic 50.0%
!! TCTCGTCGGCTTGCTCAATA+GGG - chr2.1:46810399-46810418 None:intergenic 50.0%
ACGAGTAGCACTGCTGCCTT+CGG - chr2.1:46809677-46809696 None:intergenic 55.0%
AGAAGATGCTGAAGAGCCCG+AGG + chr2.1:46810177-46810196 MS.gene052998:CDS 55.0%
AGCCAACCGAGCACATGCTT+CGG - chr2.1:46809155-46809174 None:intergenic 55.0%
AGCGGAGGAGGAAAAGGAGA+GGG + chr2.1:46809293-46809312 MS.gene052998:CDS 55.0%
AGTAAGGCCTGCACTTCCAG+GGG - chr2.1:46809933-46809952 None:intergenic 55.0%
ATTGCTGCCCACAAGGTCAC+TGG - chr2.1:46809647-46809666 None:intergenic 55.0%
CCCCTGGACGCAATTTCCTA+GGG - chr2.1:46809054-46809073 None:intergenic 55.0%
CCCTAGGAAATTGCGTCCAG+GGG + chr2.1:46809051-46809070 MS.gene052998:CDS 55.0%
CTACGAAGGGAACTCCTCTC+AGG + chr2.1:46810002-46810021 MS.gene052998:CDS 55.0%
GACCCTAGGAAATTGCGTCC+AGG + chr2.1:46809049-46809068 MS.gene052998:CDS 55.0%
GAGTAAGGCCTGCACTTCCA+GGG - chr2.1:46809934-46809953 None:intergenic 55.0%
GCCCGAGGAGAAGATTGAAG+AGG + chr2.1:46810192-46810211 MS.gene052998:CDS 55.0%
GCGACCCGATCCTGTTGATA+TGG + chr2.1:46809105-46809124 MS.gene052998:CDS 55.0%
GCTTGCTCAATAGGGGTTGG+TGG - chr2.1:46810391-46810410 None:intergenic 55.0%
GGATTCGGATCAATCTCCCC+TGG - chr2.1:46809070-46809089 None:intergenic 55.0%
GGCAGCAATGAACTCACCGA+AGG + chr2.1:46809658-46809677 MS.gene052998:CDS 55.0%
GGGTTATGCCAGTGACCTTG+TGG + chr2.1:46809636-46809655 MS.gene052998:CDS 55.0%
TAAGGCAGCGGAGGAGGAAA+AGG + chr2.1:46809287-46809306 MS.gene052998:CDS 55.0%
TCCCCTGGACGCAATTTCCT+AGG - chr2.1:46809055-46809074 None:intergenic 55.0%
TGCTGATGAGGATAGGGAGG+TGG + chr2.1:46809375-46809394 MS.gene052998:CDS 55.0%
TGGCATGCAGAATAGCAGCC+GGG - chr2.1:46809519-46809538 None:intergenic 55.0%
TTCCGAAGCATGTGCTCGGT+TGG + chr2.1:46809150-46809169 MS.gene052998:CDS 55.0%
TTGCAGAGGGAGTCAGCAGT+GGG - chr2.1:46809910-46809929 None:intergenic 55.0%
! AAGGCTTGGTTGGGTGTCTG+TGG - chr2.1:46809718-46809737 None:intergenic 55.0%
! AGAAGAAGAGGCACGGCAGA+AGG + chr2.1:46810246-46810265 MS.gene052998:CDS 55.0%
! AGGCCCATTTGCTGCTCGTT+TGG - chr2.1:46810512-46810531 None:intergenic 55.0%
! CGAAGGAGTACGACCCACAA+TGG - chr2.1:46808957-46808976 None:intergenic 55.0%
! GACTGAGTGGACTCGAGAAG+AGG + chr2.1:46808871-46808890 MS.gene052998:CDS 55.0%
! GCTCGGTTGGCTAACACGAA+AGG + chr2.1:46809163-46809182 MS.gene052998:CDS 55.0%
! TCGGCTTGCTCAATAGGGGT+TGG - chr2.1:46810394-46810413 None:intergenic 55.0%
!! AAGTGGTATGGCGCTGGCAA+GGG + chr2.1:46809958-46809977 MS.gene052998:CDS 55.0%
!! CTCGTCGGCTTGCTCAATAG+GGG - chr2.1:46810398-46810417 None:intergenic 55.0%
AGAGATGCTGGAGGAGGCCT+AGG - chr2.1:46810313-46810332 None:intergenic 60.0%
AGCTCCAGAGATGCTGGAGG+AGG - chr2.1:46810319-46810338 None:intergenic 60.0%
CAGCGGAGGAGGAAAAGGAG+AGG + chr2.1:46809292-46809311 MS.gene052998:CDS 60.0%
GAGGGAGTCAGCAGTGGGAT+TGG - chr2.1:46809905-46809924 None:intergenic 60.0%
GCACTTCCAGGGGTTGCAGA+GGG - chr2.1:46809923-46809942 None:intergenic 60.0%
GCAGGCCTTACTCCCAGAAG+TGG + chr2.1:46809941-46809960 MS.gene052998:intron 60.0%
GCCAGCGCCATACCACTTCT+GGG - chr2.1:46809956-46809975 None:intergenic 60.0%
GGAGTAAGGCCTGCACTTCC+AGG - chr2.1:46809935-46809954 None:intergenic 60.0%
GGCATGCAGAATAGCAGCCG+GGG - chr2.1:46809518-46809537 None:intergenic 60.0%
GTTGCAGAGGGAGTCAGCAG+TGG - chr2.1:46809911-46809930 None:intergenic 60.0%
TAGGCCTCCTCCAGCATCTC+TGG + chr2.1:46810312-46810331 MS.gene052998:CDS 60.0%
TCCCAGAAGTGGTATGGCGC+TGG + chr2.1:46809952-46809971 MS.gene052998:CDS 60.0%
TGCACTTCCAGGGGTTGCAG+AGG - chr2.1:46809924-46809943 None:intergenic 60.0%
TGCAGAGAGGCAAGATGCCC+CGG + chr2.1:46809498-46809517 MS.gene052998:CDS 60.0%
TGCCAGCGCCATACCACTTC+TGG - chr2.1:46809957-46809976 None:intergenic 60.0%
! TCTGCAACCCCTGGAAGTGC+AGG + chr2.1:46809923-46809942 MS.gene052998:intron 60.0%
! TGTCTGAGACTCCCTCAGCC+TGG - chr2.1:46809815-46809834 None:intergenic 60.0%
!! GAAGTGGTATGGCGCTGGCA+AGG + chr2.1:46809957-46809976 MS.gene052998:CDS 60.0%
CTGACTCCCTCTGCAACCCC+TGG + chr2.1:46809914-46809933 MS.gene052998:intron 65.0%
GGTCGCGCAGGCTTCGATTC+AGG - chr2.1:46809091-46809110 None:intergenic 65.0%
TCAACAGGATCGGGTCGCGC+AGG - chr2.1:46809103-46809122 None:intergenic 65.0%
!! GGTATGGCGCTGGCAAGGGA+TGG + chr2.1:46809962-46809981 MS.gene052998:CDS 65.0%


Chromosome Type Strat End Strand Name
chr2.1 gene 46808707 46811536 46808707 ID=MS.gene052998
chr2.1 mRNA 46808707 46811536 46808707 ID=MS.gene052998.t1;Parent=MS.gene052998
chr2.1 exon 46808707 46809871 46808707 ID=MS.gene052998.t1.exon1;Parent=MS.gene052998.t1
chr2.1 CDS 46808707 46809871 46808707 ID=cds.MS.gene052998.t1;Parent=MS.gene052998.t1
chr2.1 exon 46809945 46810576 46809945 ID=MS.gene052998.t1.exon2;Parent=MS.gene052998.t1
chr2.1 CDS 46809945 46810576 46809945 ID=cds.MS.gene052998.t1;Parent=MS.gene052998.t1
chr2.1 exon 46811240 46811536 46811240 ID=MS.gene052998.t1.exon3;Parent=MS.gene052998.t1
chr2.1 CDS 46811240 46811536 46811240 ID=cds.MS.gene052998.t1;Parent=MS.gene052998.t1
Gene Sequence

>MS.gene052998

ATGAGCATAGGTGTTTGGACAAACACCGAGGATGAAATCCTGAAAGCTGCTGTTATGAAATATGGTAAAAATCAGTGGGCTCATATCTCGTCCCTACTCGTTTGCAAATCTGCAGAACGGTGTAAAGCTCGTTGGTATGAGTGGTTAGATCCTTCCATTAAGAAGACTGAGTGGACTCGAGAAGAGGATGAGAAGCTACTTCATCTTGCTAAGCTTACGCCTACTCAGTGGAGAACCATTAGTGATACCATTGTGGGTCGTACTCCTTCGCAGTGTCTTGAACGGTATGAGAAACTCCTTGACGCCACTTGTGTTAAGGACGATAACTATGAACCTGGTGATGACCCTAGGAAATTGCGTCCAGGGGAGATTGATCCGAATCCTGAATCGAAGCCTGCGCGACCCGATCCTGTTGATATGGATGAGTATGAGAAGGAGATGCTTTCCGAAGCATGTGCTCGGTTGGCTAACACGAAAGGTAAAAAAGCAAAAAGGAAAGCTAGGGAGAAACAGCTTGAGGAAGCAAGGAGGCTTGCTTCTTTACAGAAAAAGAGAGAATTGAAGGCTGCTGGGATTGATATAAGGCAGCGGAGGAGGAAAAGGAGAGGGATTGATTATAATGCAGAAATTCCGTTTGAGAAAAGGCCTCCTTTGGGTTATTTTGATGTTGCTGATGAGGATAGGGAGGTGGAGCAGCATGCATTCCCTACTACTATAGAAGAACTTGAGGGGAAAAGAAGGATTGATGTTGAGGCTCAGTTGAGAAAGCAAGACGTTGCAAGGAATAAAATTGCAGAGAGGCAAGATGCCCCGGCTGCTATTCTGCATGCCAAGAAGCTGAATGACCCAGAAACAGTTGGAAAGAGATCAAAACTGATGCTTCCACCACCTCAGATTTCCGATCAAGAATTGGATGAAATTGCCAAGTTGGGTTATGCCAGTGACCTTGTGGGCAGCAATGAACTCACCGAAGGCAGCAGTGCTACTCGTGCTCTTCTCCCTAATTATCCACAGACACCCAACCAAGCCTTGACTCCTTTAAGAACTCCTCAGAGAACACCAGCTAGTAAAGGAGATGCTATCATGATGGAAGCTGAAAATTTGGCCAGGCTGAGGGAGTCTCAGACACCACTGTTAGGAGTTGCATCCTTCAGATTTTTCTGGGGTCACTCCTAAGAAAAAGGAAATTCACATTCCAATCCCACTGCTGACTCCCTCTGCAACCCCTGGAAGTGCAGGCCTTACTCCCAGAAGTGGTATGGCGCTGGCAAGGGATGGTTTTTCTTTTGGCATGACTACGAAGGGAACTCCTCTCAGGGATGAGCTACATATCAATGAAGATATGGAGATGCGTGATAGTGCTAGACTCGAGCTACAGAGACAAGCTGATATGAAAAAAAGTCTGCGCTCTGGTTTAAGCAGCCTTCCACAGCCTAAAAATGAGTACCAGATAGTGATGCAACCAATTCAAGAAGATGCTGAAGAGCCCGAGGAGAAGATTGAAGAGGATATGTCTGATAGAATGGCTAGAGAAAAGGCAGAAGAAGAGGCACGGCAGAAGGCGTTACTTAGAAAACGATCAAAAGTCATTCAAAGGGAACTTCCTAGGCCTCCTCCAGCATCTCTGGAGCTTATTAGGAACTCCTTGATAAGAGCTGACGGAGACAAAAGTTCATTTGTTCCACCAACCCCTATTGAGCAAGCCGACGAGATGATAAGAAAGGAGCTTCCAGCCTTACTGGAGCATGACAATGGTAAGTATCCGCTTGAGGAGATAGCAAATAAGGAGAGAGAGAAAGGATCCAAACGAGCAGCAAATGGGCCTGCTATCCCTGTCATAGAAGATTATCAGGAGGACGAGATGAAATCTGTATGTTTTTACTAATTTATCTGCTCACCTTTAATTTTATTTTTATGCCTTTTACTTCGCTTTTCTTCATTTGCTACTAAGTAGCTTGTTCACCTTTTCACCCTTTGGGGTACACAACGTGATTTTTCATTTAGATGATACACATGCTTTTTCTGATGATCTTGATCTTGAAATTATTAGTACTTTGTCCGATTATTAATTACTTGGCATCATCATTTTTATGCTGAAACCCCTTTTCACCCCTTTGAAACCACTGGAATCTTATGTAAAAATTAAATAGAAAAATTTGGACATCTTTTTAATTTATGTATCATCTCCTTTATGAGCTAGTTGCATATTATGGTGGTATGTAATATGTATTGTATCGTCCTTGTTTTTTTTCAGTTATAATCATTTAATTAATTAGGTTGAAGTTAGTTTTAGTTTCTCGCAATTGTTGTTCTCTATTTCTTTCGTTTTTGTAACAAGTGATTCTTCAATTGCCTTTTACAACATTTATTCCCTATTTACGTTAATTCGCCGAATGATAGTAATACAAACTTCCAAACTGCTTGTTAATTATTATTTGACTCCTTAAATTTGACTATGGAGATGAGCTTGTACGTTGATGAATGATAATATCTGTGTTGACTGTTATTTATTTTTATCTGTTGTTCTCAATAGGCTGATCAGTTGATAAAAGATGAAGCCCAGTATCTTCGTGAGGCAATGGGGCATGAAAATGATTCCCTCGAGGATTTTGTTGAAGCACATACAACATGCATTAATGATTTAATGTACTTTGATACCCGTAACACACATGGTCTATCAAGTGTTGCTGGAAACGCAGAGAAGCTAGCAGCTTTGCAGAATGAATTTGAGAATGTGAGAAGTAAGCTGGATGATGGGAAGGAGAAGATGATTCGACTTGAGAAGAAGGTTACATTACTCACGCAAGGGTACGAGTTATGCTACTGCTAA
Protein sequence

>MS.gene052998.t1

MSIGVWTNTEDEILKAAVMKYGKNQWAHISSLLVCKSAERCKARWYEWLDPSIKKTEWTREEDEKLLHLAKLTPTQWRTISDTIVGRTPSQCLERYEKLLDATCVKDDNYEPGDDPRKLRPGEIDPNPESKPARPDPVDMDEYEKEMLSEACARLANTKGKKAKRKAREKQLEEARRLASLQKKRELKAAGIDIRQRRRKRRGIDYNAEIPFEKRPPLGYFDVADEDREVEQHAFPTTIEELEGKRRIDVEAQLRKQDVARNKIAERQDAPAAILHAKKLNDPETVGKRSKLMLPPPQISDQELDEIAKLGYASDLVGSNELTEGSSATRALLPNYPQTPNQALTPLRTPQRTPASKGDAIMMEAENLARLRESQTPLLGVASFRFFWGLTPRSGMALARDGFSFGMTTKGTPLRDELHINEDMEMRDSARLELQRQADMKKSLRSGLSSLPQPKNEYQIVMQPIQEDAEEPEEKIEEDMSDRMAREKAEEEARQKALLRKRSKVIQRELPRPPPASLELIRNSLIRADGDKSSFVPPTPIEQADEMIRKELPALLEHDNGKYPLEEIANKEREKGSKRAANGPAIPVIEDYQEDEMKSADQLIKDEAQYLREAMGHENDSLEDFVEAHTTCINDLMYFDTRNTHGLSSVAGNAEKLAALQNEFENVRSKLDDGKEKMIRLEKKVTLLTQGYELCYC