Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene068959.t1 | XP_003628406.2 | 93.7 | 575 | 36 | 0 | 3 | 577 | 14 | 588 | 0.00E+00 | 1107.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene068959.t1 | Q93X23 | 50.4 | 565 | 273 | 4 | 17 | 577 | 32 | 593 | 2.0e-159 | 563.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene068959.t1 | G7LB17 | 93.7 | 575 | 36 | 0 | 3 | 577 | 14 | 588 | 0.0e+00 | 1107.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene055359 | MS.gene068959 | 0.818921 | 1.52E-52 | -1.69E-46 |
| MS.gene059632 | MS.gene068959 | 0.80934 | 1.99E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 87 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCCTAATTTCTTTGTTAAAA+TGG | 0.095292 | 8.1:+57847397 | None:intergenic |
| TTTACTCCAATTCGCTAAAT+TGG | 0.181502 | 8.1:-57840043 | MS.gene068959:CDS |
| CTAAATTCCAACCTTCAATT+TGG | 0.197481 | 8.1:-57847726 | MS.gene068959:CDS |
| GTTTGAAGATGAAACTATAT+TGG | 0.261667 | 8.1:-57840244 | MS.gene068959:CDS |
| CTTTGGATCTTCCATTGAAT+TGG | 0.264293 | 8.1:-57840134 | MS.gene068959:CDS |
| ATTCTGAATTGTTCAAAATC+TGG | 0.272530 | 8.1:+57838334 | None:intergenic |
| ATGCACACTTTAGTTCTTCT+AGG | 0.272677 | 8.1:+57839995 | None:intergenic |
| ATCATCAACTGTGGTAATTA+AGG | 0.283666 | 8.1:+57838291 | None:intergenic |
| TACTTTATATTCACTACTTA+AGG | 0.308038 | 8.1:+57847684 | None:intergenic |
| AAGCATATTTGCATGTAGTA+TGG | 0.312552 | 8.1:+57838084 | None:intergenic |
| TGAAATCCAATTTAGCGAAT+TGG | 0.313439 | 8.1:+57840037 | None:intergenic |
| AGTTGCTTACTGCTTTCTTC+AGG | 0.318825 | 8.1:+57837983 | None:intergenic |
| GAGATCAACGCAAAGTTCTA+AGG | 0.332792 | 8.1:-57847509 | MS.gene068959:CDS |
| CAAAGAAATTAGGGACATAT+TGG | 0.340628 | 8.1:-57847388 | MS.gene068959:CDS |
| AGTCCATGATGTCTACATTA+TGG | 0.344971 | 8.1:-57835803 | MS.gene068959:CDS |
| TATGATGCCTTGCATTGAAT+TGG | 0.356253 | 8.1:+57847793 | None:intergenic |
| AAATGCATTATGAACGAAAC+TGG | 0.357987 | 8.1:-57835858 | MS.gene068959:CDS |
| TGAAGAGCTCAAGTTCTTCC+AGG | 0.364896 | 8.1:+57838248 | None:intergenic |
| AGAGATAACGGCCGGTTGTT+AGG | 0.375487 | 8.1:+57847844 | None:intergenic |
| ATGGAAGACTTGGTTCGTTT+AGG | 0.382342 | 8.1:-57837369 | MS.gene068959:CDS |
| ATAATCACGAGCCTCTATTT+CGG | 0.397407 | 8.1:+57835830 | None:intergenic |
| GGACAGTTTGTAGATGTTCA+TGG | 0.404960 | 8.1:-57840300 | MS.gene068959:CDS |
| ACTTCTAATAAGTCATGCTT+TGG | 0.407606 | 8.1:-57840151 | MS.gene068959:CDS |
| TCTTCACAGAAGTTATTGAT+AGG | 0.419701 | 8.1:-57838231 | MS.gene068959:intron |
| GAATTGTTGAGAACTTTGTT+TGG | 0.420249 | 8.1:-57838378 | MS.gene068959:CDS |
| ATATTCTTTGAGAAAATTTG+TGG | 0.422729 | 8.1:+57840203 | None:intergenic |
| TTAATGCAGGAAGAAATTTA+CGG | 0.426943 | 8.1:-57847531 | MS.gene068959:intron |
| GTTGAAGAATACATGGCAAA+TGG | 0.426945 | 8.1:-57837459 | MS.gene068959:CDS |
| TGTATGTTCATGTACCAGTA+TGG | 0.427645 | 8.1:-57835702 | MS.gene068959:CDS |
| TGCTATAAGCATCTCCATAC+TGG | 0.428412 | 8.1:+57835688 | None:intergenic |
| ATAATGCATTTATTTAACTT+TGG | 0.429400 | 8.1:+57835870 | None:intergenic |
| GAGAACTTTGTTTGGAATGT+TGG | 0.432073 | 8.1:-57838370 | MS.gene068959:CDS |
| CTCTTGCAATGGTTGCTGTC+AGG | 0.432621 | 8.1:+57837325 | None:intergenic |
| CGAGAAATTGTCCAATTCAA+TGG | 0.434579 | 8.1:+57840123 | None:intergenic |
| TAATCATTGGTCCAAATTGA+AGG | 0.438142 | 8.1:+57847715 | None:intergenic |
| GATGCCACATTCAATCGAAA+TGG | 0.438499 | 8.1:-57837388 | MS.gene068959:CDS |
| ATTGATGTATTGCAAAGGAT+TGG | 0.446275 | 8.1:-57847429 | MS.gene068959:CDS |
| TAGATGGGATTTGAATGCCT+TGG | 0.458301 | 8.1:-57838115 | MS.gene068959:CDS |
| TTAAAGAGTGAAAATGAGAC+AGG | 0.459331 | 8.1:-57835900 | MS.gene068959:intron |
| TGCACAACACTTCAGGGAAT+CGG | 0.460962 | 8.1:+57835618 | None:intergenic |
| AAATACAGCATGTGCACCTA+TGG | 0.461919 | 8.1:+57837428 | None:intergenic |
| GATGATGTGTATGATTTGTA+TGG | 0.476992 | 8.1:-57838274 | MS.gene068959:CDS |
| CATGGTTATGAGATATCTAC+AGG | 0.495263 | 8.1:-57847285 | MS.gene068959:intron |
| AAACGCTTTCTCCTAACAAC+CGG | 0.495343 | 8.1:-57847855 | MS.gene068959:CDS |
| TAAGGACTGAATATAATCAT+TGG | 0.497500 | 8.1:+57847702 | None:intergenic |
| CTTACTGCTTTCTTCAGGTA+CGG | 0.500237 | 8.1:+57837988 | None:intergenic |
| AACTGGAGCTTCCGAAATAG+AGG | 0.500437 | 8.1:-57835841 | MS.gene068959:CDS |
| GCCATGTATTCTTCAACGGT+TGG | 0.503430 | 8.1:+57837465 | None:intergenic |
| CATTGGTCCAAATTGAAGGT+TGG | 0.506340 | 8.1:+57847719 | None:intergenic |
| GAAGCAAAGTGGTACTATAG+TGG | 0.511242 | 8.1:-57837495 | MS.gene068959:CDS |
| GCAAGAGTTGCTAATGACTA+CGG | 0.511511 | 8.1:-57837309 | MS.gene068959:CDS |
| TTCAATCGAAATGGAAGACT+TGG | 0.513598 | 8.1:-57837379 | MS.gene068959:CDS |
| TTGAAGAATACATGGCAAAT+GGG | 0.520906 | 8.1:-57837458 | MS.gene068959:CDS |
| TGCTGTCAGGCGAATTATGT+TGG | 0.521802 | 8.1:+57837338 | None:intergenic |
| CTTCCATAATGTAGACATCA+TGG | 0.522455 | 8.1:+57835800 | None:intergenic |
| AGCATATTTGCATGTAGTAT+GGG | 0.525197 | 8.1:+57838085 | None:intergenic |
| TTGCATGTTGCTAGAGATAA+CGG | 0.527851 | 8.1:+57847832 | None:intergenic |
| GAATATTTGAAACAAAATAG+AGG | 0.535112 | 8.1:-57840186 | MS.gene068959:CDS |
| TTTCTCGACTCGAGGCTCGA+TGG | 0.546070 | 8.1:-57840107 | MS.gene068959:CDS |
| CCCTCGTCCAATTCAATGCA+AGG | 0.550879 | 8.1:-57847800 | MS.gene068959:CDS |
| ATTTGCCATGTATTCTTCAA+CGG | 0.557754 | 8.1:+57837461 | None:intergenic |
| GTCATTAGCAACTCTTGCAA+TGG | 0.561701 | 8.1:+57837314 | None:intergenic |
| CAAGAGTTGCTAATGACTAC+GGG | 0.573530 | 8.1:-57837308 | MS.gene068959:CDS |
| ATTGAAGGTTGGAATTTAGA+CGG | 0.575521 | 8.1:+57847730 | None:intergenic |
| TTCAGACTCTTGAGGCAACA+TGG | 0.578812 | 8.1:-57847303 | MS.gene068959:CDS |
| TCTTCCATTTCGATTGAATG+TGG | 0.582432 | 8.1:+57837384 | None:intergenic |
| TCCAACCGTTGAAGAATACA+TGG | 0.583771 | 8.1:-57837466 | MS.gene068959:CDS |
| CATATTTGATAGAAGCAAAG+TGG | 0.585970 | 8.1:-57837506 | MS.gene068959:CDS |
| TTGGACAATTTCTCGACTCG+AGG | 0.586359 | 8.1:-57840115 | MS.gene068959:CDS |
| AGATCAACGCAAAGTTCTAA+GGG | 0.587082 | 8.1:-57847508 | MS.gene068959:CDS |
| AAGAACTAAAGTGTGCATCA+AGG | 0.590523 | 8.1:-57839990 | MS.gene068959:intron |
| GCACATGCTGTATTTACCCA+TGG | 0.601527 | 8.1:-57837420 | MS.gene068959:CDS |
| AGTTTATTGATGTATTGCAA+AGG | 0.602279 | 8.1:-57847434 | MS.gene068959:CDS |
| GAAGAGCTCAAGTTCTTCCA+GGG | 0.609664 | 8.1:+57838249 | None:intergenic |
| GTAGTATGGGAGAGAATCCA+AGG | 0.610000 | 8.1:+57838098 | None:intergenic |
| GCCTTGCATTGAATTGGACG+AGG | 0.610175 | 8.1:+57847799 | None:intergenic |
| GTGGCATCGAAAAGTAACCA+TGG | 0.621565 | 8.1:+57837403 | None:intergenic |
| TGGCATCGAAAAGTAACCAT+GGG | 0.626709 | 8.1:+57837404 | None:intergenic |
| CATGCAAATATGCTTTCATG+CGG | 0.630142 | 8.1:-57838076 | MS.gene068959:CDS |
| CACTCAAGTTCAGACTCTTG+AGG | 0.650504 | 8.1:-57847311 | MS.gene068959:CDS |
| ATGTTGCTAGAGATAACGGC+CGG | 0.651350 | 8.1:+57847836 | None:intergenic |
| GAGTCTGAACTTGAGTGATG+TGG | 0.655823 | 8.1:+57847317 | None:intergenic |
| CCTTGCATTGAATTGGACGA+GGG | 0.668480 | 8.1:+57847800 | None:intergenic |
| GCAAATGGGTGTGTATCCAT+AGG | 0.671102 | 8.1:-57837444 | MS.gene068959:CDS |
| GTATGATTTGTATGGCACCC+TGG | 0.671543 | 8.1:-57838266 | MS.gene068959:CDS |
| ATCATACACATCATCAACTG+TGG | 0.683052 | 8.1:+57838282 | None:intergenic |
| GACTTATTAGAAGTGACACG+TGG | 0.737197 | 8.1:+57840160 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.1 | gene | 57835629 | 57847889 | 57835629 | ID=MS.gene068959 |
| chr8.1 | mRNA | 57835629 | 57847889 | 57835629 | ID=MS.gene068959.t1;Parent=MS.gene068959 |
| chr8.1 | exon | 57847687 | 57847889 | 57847687 | ID=MS.gene068959.t1.exon1;Parent=MS.gene068959.t1 |
| chr8.1 | CDS | 57847687 | 57847889 | 57847687 | ID=cds.MS.gene068959.t1;Parent=MS.gene068959.t1 |
| chr8.1 | exon | 57847286 | 57847544 | 57847286 | ID=MS.gene068959.t1.exon2;Parent=MS.gene068959.t1 |
| chr8.1 | CDS | 57847286 | 57847544 | 57847286 | ID=cds.MS.gene068959.t1;Parent=MS.gene068959.t1 |
| chr8.1 | exon | 57839991 | 57840366 | 57839991 | ID=MS.gene068959.t1.exon3;Parent=MS.gene068959.t1 |
| chr8.1 | CDS | 57839991 | 57840366 | 57839991 | ID=cds.MS.gene068959.t1;Parent=MS.gene068959.t1 |
| chr8.1 | exon | 57838232 | 57838450 | 57838232 | ID=MS.gene068959.t1.exon4;Parent=MS.gene068959.t1 |
| chr8.1 | CDS | 57838232 | 57838450 | 57838232 | ID=cds.MS.gene068959.t1;Parent=MS.gene068959.t1 |
| chr8.1 | exon | 57837993 | 57838134 | 57837993 | ID=MS.gene068959.t1.exon5;Parent=MS.gene068959.t1 |
| chr8.1 | CDS | 57837993 | 57838134 | 57837993 | ID=cds.MS.gene068959.t1;Parent=MS.gene068959.t1 |
| chr8.1 | exon | 57837299 | 57837547 | 57837299 | ID=MS.gene068959.t1.exon6;Parent=MS.gene068959.t1 |
| chr8.1 | CDS | 57837299 | 57837547 | 57837299 | ID=cds.MS.gene068959.t1;Parent=MS.gene068959.t1 |
| chr8.1 | exon | 57835629 | 57835916 | 57835629 | ID=MS.gene068959.t1.exon7;Parent=MS.gene068959.t1 |
| chr8.1 | CDS | 57835629 | 57835916 | 57835629 | ID=cds.MS.gene068959.t1;Parent=MS.gene068959.t1 |
| Gene Sequence |
| Protein sequence |
