AlfalfaGEDB | MS.gene069000

M. sativa cultivar XinJiangDaYe / MS.gene069000

KEGG: K14496
GO: -
PFAM: PF10604.12

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene069000.t1	XP_003623366.1	98.9	186	2	0	1	186	1	186	8.90E-100	372.9

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene069000.t1	O80920	74.6	177	36	2	5	180	31	199	3.7e-70	265.8

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene069000.t1	G7KT83	98.9	186	2	0	1	186	1	186	6.4e-100	372.9

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene32980	MS.gene069000	PPI
MS.gene069000	MS.gene24547	PPI
MS.gene051624	MS.gene069000	PPI
MS.gene069000	MS.gene57223	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene069000.t1	MTR_7g070050	98.925	186	2	0	1	186	1	186	1.76e-136	379
MS.gene069000.t1	MTR_4g014460	68.715	179	50	3	1	178	36	209	2.55e-86	253
MS.gene069000.t1	MTR_3g071740	66.857	175	49	3	4	177	57	223	1.23e-79	237
MS.gene069000.t1	MTR_5g083270	66.102	177	50	4	4	178	37	205	5.18e-76	226
MS.gene069000.t1	MTR_8g027805	55.689	167	68	3	13	179	18	178	2.26e-64	196
MS.gene069000.t1	MTR_1g028380	56.805	169	67	3	11	179	17	179	4.75e-64	196
MS.gene069000.t1	MTR_3g090980	54.913	173	72	3	7	179	15	181	7.88e-63	193
MS.gene069000.t1	MTR_5g030500	58.182	165	66	2	13	176	44	206	1.05e-60	188
MS.gene069000.t1	MTR_1g016480	52.023	173	78	3	13	185	39	206	1.88e-59	184

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene069000.t1	AT2G38310	74.576	177	36	2	5	180	31	199	3.97e-85	249
MS.gene069000.t1	AT2G40330	67.059	170	52	3	9	177	44	210	3.95e-81	240
MS.gene069000.t1	AT5G05440	66.867	166	46	3	9	173	41	198	5.46e-77	229
MS.gene069000.t1	AT1G01360	56.471	170	68	2	16	185	24	187	1.70e-66	202
MS.gene069000.t1	AT5G53160	52.809	178	77	3	2	179	9	179	2.48e-64	196
MS.gene069000.t1	AT4G01026	56.707	164	65	3	17	180	27	184	1.88e-62	192
MS.gene069000.t1	AT2G26040	54.167	168	68	2	8	172	17	178	4.15e-60	186
MS.gene069000.t1	AT4G17870	51.111	180	82	2	1	179	5	179	6.55e-59	182
MS.gene069000.t1	AT4G27920	47.159	176	88	2	11	186	12	182	1.19e-57	179
MS.gene069000.t1	AT1G73000	50.000	168	78	3	11	172	34	201	1.29e-54	172
MS.gene069000.t1	AT5G45860	55.758	165	60	2	15	179	6	157	7.25e-51	161
MS.gene069000.t1	AT5G46790	53.179	173	78	2	7	178	38	208	3.95e-49	159
MS.gene069000.t1	AT5G46790	53.179	173	78	2	7	178	38	208	3.95e-49	159
MS.gene069000.t1	AT5G45870	55.556	153	61	1	26	178	10	155	2.92e-48	154
MS.gene069000.t1	AT4G18620	51.553	161	67	3	24	178	7	162	8.94e-40	133
MS.gene069000.t1	AT5G53160	53.571	112	50	2	2	113	9	118	1.93e-37	127

Find 67 sgRNAs with CRISPR-Local

Find 72 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CGCGAAATTTGCTGAGAATT+TGG	0.108745	7.2:-33590976	MS.gene069000:CDS
TGGTAGCGGGCGATGGCGTC+GGG	0.286221	7.2:+33591461	None:intergenic
GGGACGGTGGTTGTTGGGTT+TGG	0.316747	7.2:+33591482	None:intergenic
GTGGTAGCGGGCGATGGCGT+CGG	0.350975	7.2:+33591460	None:intergenic
GCGTCGGGGACGGTGGTTGT+TGG	0.355449	7.2:+33591476	None:intergenic
ACGGCGGAGCAACACTGGTT+GGG	0.355563	7.2:+33591422	None:intergenic
GTATCTTCAGTGGTGTTTCC+AGG	0.376991	7.2:+33591044	None:intergenic
CGTCGGGGACGGTGGTTGTT+GGG	0.383221	7.2:+33591477	None:intergenic
TACGTTGTTGATGTTCCTCC+TGG	0.394303	7.2:-33591062	MS.gene069000:CDS
GTCATCAGCTTCAGCATGAT+TGG	0.416594	7.2:-33591191	MS.gene069000:CDS
CGTTCGGTGCTGACGGCGGC+GGG	0.423682	7.2:+33591242	None:intergenic
TCTTCGTCGGAGATCGGACT+AGG	0.424642	7.2:+33591122	None:intergenic
GACTGCGTGGGTGTGGTAGC+GGG	0.430031	7.2:+33591448	None:intergenic
CAAATTTCGCGAGAGATTGA+AGG	0.431244	7.2:+33590988	None:intergenic
AGACTGCGTGGGTGTGGTAG+CGG	0.434046	7.2:+33591447	None:intergenic
ACGTTCGGTGCTGACGGCGG+CGG	0.440590	7.2:+33591241	None:intergenic
GCGGGCGATGGCGTCGGGGA+CGG	0.442801	7.2:+33591466	None:intergenic
GCTGACGGCGGCGGGGAGGC+CGG	0.449916	7.2:+33591250	None:intergenic
CGGCGGAGCAACACTGGTTG+GGG	0.450760	7.2:+33591423	None:intergenic
CCGCACCAGTCTCCACTGTC+TGG	0.459053	7.2:-33591385	MS.gene069000:CDS
GCATGATTGGCGGTGATCAC+CGG	0.466189	7.2:-33591178	MS.gene069000:CDS
CGTGAAGTCCGCGTCATCTC+CGG	0.470592	7.2:-33591269	MS.gene069000:CDS
GTGGGTGTGGTAGCGGGCGA+TGG	0.478652	7.2:+33591454	None:intergenic
GTCTAGGACTTCGAGACGTT+CGG	0.489158	7.2:+33591226	None:intergenic
CGGAGATGACGCGGACTTCA+CGG	0.493833	7.2:+33591270	None:intergenic
GGCGATGGCGTCGGGGACGG+TGG	0.507167	7.2:+33591469	None:intergenic
TGTTTGTTGATACTATTCTT+CGG	0.519630	7.2:-33591016	MS.gene069000:CDS
GACGGCGGAGCAACACTGGT+TGG	0.519929	7.2:+33591421	None:intergenic
TTGCCATCTTCGTCGGAGAT+CGG	0.523840	7.2:+33591116	None:intergenic
AGTCCGATCTCCGACGAAGA+TGG	0.524898	7.2:-33591119	MS.gene069000:CDS
ACGGACCAGACAGTGGAGAC+TGG	0.529100	7.2:+33591380	None:intergenic
TTCGAGACGTTCGGTGCTGA+CGG	0.530715	7.2:+33591235	None:intergenic
TGCCATGTCATCCTCGGCGA+CGG	0.538063	7.2:-33591308	MS.gene069000:CDS
CGGGGAGGCCGGAGATGACG+CGG	0.540759	7.2:+33591261	None:intergenic
CGGTGCTGACGGCGGCGGGG+AGG	0.548394	7.2:+33591246	None:intergenic
GTTCGGTGCTGACGGCGGCG+GGG	0.549616	7.2:+33591243	None:intergenic
GCGTTGGTTGCCATCTTCGT+CGG	0.552270	7.2:+33591109	None:intergenic
AGACTGGTGCGGCGATATGT+TGG	0.553693	7.2:+33591396	None:intergenic
CAACCACCGTGCCGGAGCGT+TGG	0.554675	7.2:+33591093	None:intergenic
GAAGATGGCAACCAACGCTC+CGG	0.557103	7.2:-33591104	MS.gene069000:CDS
ATCCTCGGCGACGGCAACGT+CGG	0.566845	7.2:-33591299	MS.gene069000:CDS
TGACGTGACGTTCATCGTCT+AGG	0.571433	7.2:+33591210	None:intergenic
AAAAGCTGCCATGTCATCCT+CGG	0.573277	7.2:-33591314	MS.gene069000:CDS
GGACTCAACAACCACCGTGC+CGG	0.575845	7.2:+33591085	None:intergenic
ATCAGCTTCAGCATGATTGG+CGG	0.579196	7.2:-33591188	MS.gene069000:CDS
TGCCGTCGCCGAGGATGACA+TGG	0.583389	7.2:+33591306	None:intergenic
TGCCGACGTTGCCGTCGCCG+AGG	0.587984	7.2:+33591297	None:intergenic
GGATGACGGCGGAGCAACAC+TGG	0.592996	7.2:+33591417	None:intergenic
CTTCGTCGGAGATCGGACTA+GGG	0.595738	7.2:+33591123	None:intergenic
ACGGACGACGGACCAGACAG+TGG	0.618734	7.2:+33591373	None:intergenic
GGTGAAGAGTGGTGACAGAA+CGG	0.622514	7.2:+33591144	None:intergenic
TGGCAACCAACGCTCCGGCA+CGG	0.627638	7.2:-33591099	MS.gene069000:CDS
AAACACACATGTATCTTCAG+TGG	0.628787	7.2:+33591034	None:intergenic
TCGTTGATTTGATCTCGTTG+AGG	0.631063	7.2:+33590953	None:intergenic
GGTAGCGGGCGATGGCGTCG+GGG	0.638452	7.2:+33591462	None:intergenic
GGCGGAGCAACACTGGTTGG+GGG	0.641966	7.2:+33591424	None:intergenic
GAGACGTTCGGTGCTGACGG+CGG	0.644895	7.2:+33591238	None:intergenic
CAGAACGGTAGTTAGCAAGC+CGG	0.656130	7.2:+33591159	None:intergenic
GATCGGACTAGGGTGAAGAG+TGG	0.660402	7.2:+33591133	None:intergenic
TGCGGCGATATGTTGGATGA+CGG	0.663571	7.2:+33591403	None:intergenic
ATTGTCGAAGCGACGGACGA+CGG	0.667329	7.2:+33591361	None:intergenic
CTTGTGGATTGTCGAAGCGA+CGG	0.672973	7.2:+33591354	None:intergenic
CAACCAACGCTCCGGCACGG+TGG	0.673035	7.2:-33591096	MS.gene069000:CDS
TCTTCAGTGGTGTTTCCAGG+AGG	0.674870	7.2:+33591047	None:intergenic
AGGAGGAACATCAACAACGT+AGG	0.708660	7.2:+33591064	None:intergenic
GGCGATATGTTGGATGACGG+CGG	0.710240	7.2:+33591406	None:intergenic
CCAGACAGTGGAGACTGGTG+CGG	0.710710	7.2:+33591385	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TGTTTGTTGATACTATTCTT+CGG	-	chr7.2:33591422-33591441	MS.gene069000:CDS	25.0%
	AAACACACATGTATCTTCAG+TGG	+	chr7.2:33591407-33591426	None:intergenic	35.0%
!	GCTTTTGACGAAGTTTTTGT+AGG	+	chr7.2:33591110-33591129	None:intergenic	35.0%
	CAAATTTCGCGAGAGATTGA+AGG	+	chr7.2:33591453-33591472	None:intergenic	40.0%
!	ACGAAGTTTTTGTAGGCTTG+TGG	+	chr7.2:33591103-33591122	None:intergenic	40.0%
!!	CGCGAAATTTGCTGAGAATT+TGG	-	chr7.2:33591462-33591481	MS.gene069000:CDS	40.0%
!!	TCGTTGATTTGATCTCGTTG+AGG	+	chr7.2:33591488-33591507	None:intergenic	40.0%
	AAAAGCTGCCATGTCATCCT+CGG	-	chr7.2:33591124-33591143	MS.gene069000:CDS	45.0%
	AGGAGGAACATCAACAACGT+AGG	+	chr7.2:33591377-33591396	None:intergenic	45.0%
	ATCAGCTTCAGCATGATTGG+CGG	-	chr7.2:33591250-33591269	MS.gene069000:CDS	45.0%
	GTATCTTCAGTGGTGTTTCC+AGG	+	chr7.2:33591397-33591416	None:intergenic	45.0%
	GTCATCAGCTTCAGCATGAT+TGG	-	chr7.2:33591247-33591266	MS.gene069000:CDS	45.0%
	TACGTTGTTGATGTTCCTCC+TGG	-	chr7.2:33591376-33591395	MS.gene069000:CDS	45.0%
	CAGAACGGTAGTTAGCAAGC+CGG	+	chr7.2:33591282-33591301	None:intergenic	50.0%
	GGTGAAGAGTGGTGACAGAA+CGG	+	chr7.2:33591297-33591316	None:intergenic	50.0%
	TCTTCAGTGGTGTTTCCAGG+AGG	+	chr7.2:33591394-33591413	None:intergenic	50.0%
	TGACGTGACGTTCATCGTCT+AGG	+	chr7.2:33591231-33591250	None:intergenic	50.0%
	TGCGGCGATATGTTGGATGA+CGG	+	chr7.2:33591038-33591057	None:intergenic	50.0%
	TTGCCATCTTCGTCGGAGAT+CGG	+	chr7.2:33591325-33591344	None:intergenic	50.0%
!	CTTGTGGATTGTCGAAGCGA+CGG	+	chr7.2:33591087-33591106	None:intergenic	50.0%
!	GTCTAGGACTTCGAGACGTT+CGG	+	chr7.2:33591215-33591234	None:intergenic	50.0%
	AGACTGGTGCGGCGATATGT+TGG	+	chr7.2:33591045-33591064	None:intergenic	55.0%
	AGTCCGATCTCCGACGAAGA+TGG	-	chr7.2:33591319-33591338	MS.gene069000:CDS	55.0%
	ATTGTCGAAGCGACGGACGA+CGG	+	chr7.2:33591080-33591099	None:intergenic	55.0%
	CTTCGTCGGAGATCGGACTA+GGG	+	chr7.2:33591318-33591337	None:intergenic	55.0%
	GAAGATGGCAACCAACGCTC+CGG	-	chr7.2:33591334-33591353	MS.gene069000:CDS	55.0%
	GCATGATTGGCGGTGATCAC+CGG	-	chr7.2:33591260-33591279	MS.gene069000:CDS	55.0%
	TCTTCGTCGGAGATCGGACT+AGG	+	chr7.2:33591319-33591338	None:intergenic	55.0%
!	GATCGGACTAGGGTGAAGAG+TGG	+	chr7.2:33591308-33591327	None:intergenic	55.0%
!	GGCGATATGTTGGATGACGG+CGG	+	chr7.2:33591035-33591054	None:intergenic	55.0%
!!	GCGTTGGTTGCCATCTTCGT+CGG	+	chr7.2:33591332-33591351	None:intergenic	55.0%
!!	TTCGAGACGTTCGGTGCTGA+CGG	+	chr7.2:33591206-33591225	None:intergenic	55.0%
	ACGGACCAGACAGTGGAGAC+TGG	+	chr7.2:33591061-33591080	None:intergenic	60.0%
	AGACTGCGTGGGTGTGGTAG+CGG	+	chr7.2:33590994-33591013	None:intergenic	60.0%
	CCAGACAGTGGAGACTGGTG+CGG	+	chr7.2:33591056-33591075	None:intergenic	60.0%
	CGGAGATGACGCGGACTTCA+CGG	+	chr7.2:33591171-33591190	None:intergenic	60.0%
	CGTGAAGTCCGCGTCATCTC+CGG	-	chr7.2:33591169-33591188	MS.gene069000:CDS	60.0%
	GGACTCAACAACCACCGTGC+CGG	+	chr7.2:33591356-33591375	None:intergenic	60.0%
	GGGACGGTGGTTGTTGGGTT+TGG	+	chr7.2:33590959-33590978	None:intergenic	60.0%
	TGCCATGTCATCCTCGGCGA+CGG	-	chr7.2:33591130-33591149	MS.gene069000:CDS	60.0%
!	ACGGCGGAGCAACACTGGTT+GGG	+	chr7.2:33591019-33591038	None:intergenic	60.0%
	ACGGACGACGGACCAGACAG+TGG	+	chr7.2:33591068-33591087	None:intergenic	65.0%
	ACTGGTTGGGGGAGACTGCG+TGG	+	chr7.2:33591006-33591025	None:intergenic	65.0%
	ATCCTCGGCGACGGCAACGT+CGG	-	chr7.2:33591139-33591158	MS.gene069000:CDS	65.0%
	CCGCACCAGTCTCCACTGTC+TGG	-	chr7.2:33591053-33591072	MS.gene069000:CDS	65.0%
	CGTCGGGGACGGTGGTTGTT+GGG	+	chr7.2:33590964-33590983	None:intergenic	65.0%
	CTGGTTGGGGGAGACTGCGT+GGG	+	chr7.2:33591005-33591024	None:intergenic	65.0%
	GACGGCGGAGCAACACTGGT+TGG	+	chr7.2:33591020-33591039	None:intergenic	65.0%
	GACTGCGTGGGTGTGGTAGC+GGG	+	chr7.2:33590993-33591012	None:intergenic	65.0%
	TGCCGTCGCCGAGGATGACA+TGG	+	chr7.2:33591135-33591154	None:intergenic	65.0%
	TGGCAACCAACGCTCCGGCA+CGG	-	chr7.2:33591339-33591358	MS.gene069000:CDS	65.0%
!	CGGCGGAGCAACACTGGTTG+GGG	+	chr7.2:33591018-33591037	None:intergenic	65.0%
!	GGATGACGGCGGAGCAACAC+TGG	+	chr7.2:33591024-33591043	None:intergenic	65.0%
!	GGCGGAGCAACACTGGTTGG+GGG	+	chr7.2:33591017-33591036	None:intergenic	65.0%
!!	GAGACGTTCGGTGCTGACGG+CGG	+	chr7.2:33591203-33591222	None:intergenic	65.0%
	CAACCAACGCTCCGGCACGG+TGG	-	chr7.2:33591342-33591361	MS.gene069000:CDS	70.0%
	CAACCACCGTGCCGGAGCGT+TGG	+	chr7.2:33591348-33591367	None:intergenic	70.0%
	GCGTCGGGGACGGTGGTTGT+TGG	+	chr7.2:33590965-33590984	None:intergenic	70.0%
	TGGGGGAGACTGCGTGGGTG+TGG	+	chr7.2:33591000-33591019	None:intergenic	70.0%
!!	ACGTTCGGTGCTGACGGCGG+CGG	+	chr7.2:33591200-33591219	None:intergenic	70.0%
!!	GTGGGTGTGGTAGCGGGCGA+TGG	+	chr7.2:33590987-33591006	None:intergenic	70.0%
!!	GTGGTAGCGGGCGATGGCGT+CGG	+	chr7.2:33590981-33591000	None:intergenic	70.0%
!!	TGGTAGCGGGCGATGGCGTC+GGG	+	chr7.2:33590980-33590999	None:intergenic	70.0%
	CGGGGAGGCCGGAGATGACG+CGG	+	chr7.2:33591180-33591199	None:intergenic	75.0%
	TGCCGACGTTGCCGTCGCCG+AGG	+	chr7.2:33591144-33591163	None:intergenic	75.0%
!!	CGTTCGGTGCTGACGGCGGC+GGG	+	chr7.2:33591199-33591218	None:intergenic	75.0%
!!	GGTAGCGGGCGATGGCGTCG+GGG	+	chr7.2:33590979-33590998	None:intergenic	75.0%
!!	GTTCGGTGCTGACGGCGGCG+GGG	+	chr7.2:33591198-33591217	None:intergenic	75.0%
!!	GCGGGCGATGGCGTCGGGGA+CGG	+	chr7.2:33590975-33590994	None:intergenic	80.0%
!!	GGCGATGGCGTCGGGGACGG+TGG	+	chr7.2:33590972-33590991	None:intergenic	80.0%
!	GCTGACGGCGGCGGGGAGGC+CGG	+	chr7.2:33591191-33591210	None:intergenic	85.0%
!!!	CGGTGCTGACGGCGGCGGGG+AGG	+	chr7.2:33591195-33591214	None:intergenic	85.0%

Chromosome	Type	Strat	End	Strand	Name
chr7.2	gene	33590950	33591510	33590950	ID=MS.gene069000
chr7.2	mRNA	33590950	33591510	33590950	ID=MS.gene069000.t1;Parent=MS.gene069000
chr7.2	exon	33590950	33591510	33590950	ID=MS.gene069000.t1.exon1;Parent=MS.gene069000.t1
chr7.2	CDS	33590950	33591510	33590950	ID=cds.MS.gene069000.t1;Parent=MS.gene069000.t1

Gene Sequence

>MS.gene069000

ATGTTACCAAACCCAACAACCACCGTCCCCGACGCCATCGCCCGCTACCACACCCACGCAGTCTCCCCCAACCAGTGTTGCTCCGCCGTCATCCAACATATCGCCGCACCAGTCTCCACTGTCTGGTCCGTCGTCCGTCGCTTCGACAATCCACAAGCCTACAAAAACTTCGTCAAAAGCTGCCATGTCATCCTCGGCGACGGCAACGTCGGCACTCTCCGTGAAGTCCGCGTCATCTCCGGCCTCCCCGCCGCCGTCAGCACCGAACGTCTCGAAGTCCTAGACGATGAACGTCACGTCATCAGCTTCAGCATGATTGGCGGTGATCACCGGCTTGCTAACTACCGTTCTGTCACCACTCTTCACCCTAGTCCGATCTCCGACGAAGATGGCAACCAACGCTCCGGCACGGTGGTTGTTGAGTCCTACGTTGTTGATGTTCCTCCTGGAAACACCACTGAAGATACATGTGTGTTTGTTGATACTATTCTTCGGTGCAACCTTCAATCTCTCGCGAAATTTGCTGAGAATTTGGCCTCAACGAGATCAAATCAACGATAA

Protein sequence

>MS.gene069000.t1

MLPNPTTTVPDAIARYHTHAVSPNQCCSAVIQHIAAPVSTVWSVVRRFDNPQAYKNFVKSCHVILGDGNVGTLREVRVISGLPAAVSTERLEVLDDERHVISFSMIGGDHRLANYRSVTTLHPSPISDEDGNQRSGTVVVESYVVDVPPGNTTEDTCVFVDTILRCNLQSLAKFAENLASTRSNQR