Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57223.t1 | XP_003589025.2 | 100 | 189 | 0 | 0 | 1 | 189 | 18 | 206 | 2.00E-102 | 381.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57223.t1 | O80992 | 70.1 | 184 | 53 | 2 | 1 | 182 | 1 | 184 | 9.9e-71 | 267.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57223.t1 | G7IB92 | 100.0 | 189 | 0 | 0 | 1 | 189 | 18 | 206 | 1.4e-102 | 381.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene57223 | MS.gene071035 | PPI |
| MS.gene99506 | MS.gene57223 | PPI |
| MS.gene069000 | MS.gene57223 | PPI |
| MS.gene57223 | MS.gene95677 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57223.t1 | MTR_1g016480 | 100.000 | 189 | 0 | 0 | 1 | 189 | 18 | 206 | 1.93e-140 | 390 |
| MS.gene57223.t1 | MTR_5g030500 | 55.429 | 175 | 72 | 3 | 9 | 178 | 31 | 204 | 2.14e-66 | 202 |
| MS.gene57223.t1 | MTR_3g071740 | 58.333 | 156 | 57 | 3 | 26 | 179 | 72 | 221 | 1.94e-59 | 186 |
| MS.gene57223.t1 | MTR_4g014460 | 52.201 | 159 | 74 | 2 | 22 | 179 | 49 | 206 | 1.63e-53 | 170 |
| MS.gene57223.t1 | MTR_7g070050 | 52.601 | 173 | 77 | 3 | 22 | 189 | 13 | 185 | 3.24e-53 | 168 |
| MS.gene57223.t1 | MTR_8g027805 | 46.512 | 172 | 90 | 2 | 9 | 179 | 4 | 174 | 7.85e-53 | 167 |
| MS.gene57223.t1 | MTR_5g083270 | 55.333 | 150 | 60 | 3 | 33 | 179 | 57 | 202 | 1.05e-52 | 167 |
| MS.gene57223.t1 | MTR_1g028380 | 49.367 | 158 | 79 | 1 | 22 | 179 | 19 | 175 | 1.09e-52 | 167 |
| MS.gene57223.t1 | MTR_3g090980 | 46.471 | 170 | 82 | 3 | 11 | 179 | 16 | 177 | 1.37e-52 | 167 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57223.t1 | AT2G26040 | 69.312 | 189 | 54 | 3 | 1 | 187 | 1 | 187 | 1.33e-95 | 276 |
| MS.gene57223.t1 | AT1G73000 | 59.783 | 184 | 64 | 2 | 9 | 182 | 24 | 207 | 2.81e-78 | 233 |
| MS.gene57223.t1 | AT4G17870 | 54.070 | 172 | 76 | 2 | 10 | 179 | 5 | 175 | 2.53e-64 | 196 |
| MS.gene57223.t1 | AT2G40330 | 54.777 | 157 | 66 | 4 | 26 | 179 | 54 | 208 | 9.78e-55 | 173 |
| MS.gene57223.t1 | AT5G46790 | 49.711 | 173 | 83 | 2 | 10 | 178 | 32 | 204 | 4.71e-54 | 171 |
| MS.gene57223.t1 | AT5G46790 | 49.711 | 173 | 83 | 2 | 10 | 178 | 32 | 204 | 4.71e-54 | 171 |
| MS.gene57223.t1 | AT5G05440 | 55.414 | 157 | 64 | 4 | 22 | 176 | 45 | 197 | 1.27e-53 | 170 |
| MS.gene57223.t1 | AT5G53160 | 48.428 | 159 | 79 | 2 | 22 | 179 | 19 | 175 | 3.15e-52 | 166 |
| MS.gene57223.t1 | AT4G01026 | 47.170 | 159 | 81 | 2 | 22 | 179 | 23 | 179 | 9.36e-52 | 165 |
| MS.gene57223.t1 | AT1G01360 | 45.509 | 167 | 88 | 2 | 15 | 179 | 12 | 177 | 5.72e-51 | 162 |
| MS.gene57223.t1 | AT2G38310 | 56.329 | 158 | 63 | 3 | 24 | 179 | 41 | 194 | 6.43e-50 | 160 |
| MS.gene57223.t1 | AT4G27920 | 47.799 | 159 | 79 | 3 | 22 | 178 | 14 | 170 | 1.99e-46 | 151 |
| MS.gene57223.t1 | AT5G45860 | 52.258 | 155 | 64 | 3 | 23 | 176 | 5 | 150 | 7.53e-45 | 146 |
| MS.gene57223.t1 | AT5G45870 | 53.147 | 143 | 63 | 3 | 35 | 176 | 10 | 149 | 1.77e-41 | 137 |
| MS.gene57223.t1 | AT4G18620 | 47.651 | 149 | 70 | 2 | 35 | 176 | 9 | 156 | 6.63e-35 | 121 |
| MS.gene57223.t1 | AT5G53160 | 52.475 | 101 | 48 | 0 | 21 | 121 | 18 | 118 | 2.61e-34 | 119 |
Find 30 sgRNAs with CRISPR-Local
Find 33 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TACTGAAAGATTAGAGATTT+TGG | 0.170116 | 1.4:-5926950 | MS.gene57223:CDS |
| AGAGAAGTTACCGTTGTTTC+GGG | 0.231870 | 1.4:-5926991 | MS.gene57223:CDS |
| TACCGTTGTTTCGGGTTTAC+CGG | 0.281112 | 1.4:-5926983 | MS.gene57223:CDS |
| TAAGGTTTATACTATTGTTT+TGG | 0.288773 | 1.4:-5926824 | MS.gene57223:CDS |
| TAGAGAAGTTACCGTTGTTT+CGG | 0.323518 | 1.4:-5926992 | MS.gene57223:CDS |
| TTCTCAAAGCTTCTAACATA+TGG | 0.399534 | 1.4:+5927081 | None:intergenic |
| GATTGAAGTGCATGTGTTTG+AGG | 0.427491 | 1.4:+5927143 | None:intergenic |
| AAGCTTAATCTTCAGAAACT+TGG | 0.437353 | 1.4:-5926730 | MS.gene57223:CDS |
| AATGAGTTTGTCAACAATGA+AGG | 0.452521 | 1.4:-5926847 | MS.gene57223:CDS |
| GCTTCGGTGCATAATATTCA+AGG | 0.486800 | 1.4:-5927231 | MS.gene57223:CDS |
| AAACTATCGTTCGGTTACTT+CGG | 0.496046 | 1.4:-5926872 | MS.gene57223:CDS |
| TATGGCCACACTATGCTTGA+TGG | 0.499255 | 1.4:+5927099 | None:intergenic |
| GTTTGTCAACAATGAAGGTA+AGG | 0.501378 | 1.4:-5926842 | MS.gene57223:CDS |
| TTGAGGTTGGTTCTAATATG+TGG | 0.501993 | 1.4:+5927160 | None:intergenic |
| AACAGTGTCAACAAACATCT+TGG | 0.503428 | 1.4:+5926756 | None:intergenic |
| TCGTCTTCAAAACTATCGTT+CGG | 0.506349 | 1.4:-5926881 | MS.gene57223:CDS |
| GAAGTGCATGTGTTTGAGGT+TGG | 0.508894 | 1.4:+5927147 | None:intergenic |
| ATCAAAGGTTGCAACATGAA+AGG | 0.524876 | 1.4:-5927036 | MS.gene57223:CDS |
| TATATATTATTGTCCATGCA+TGG | 0.529703 | 1.4:+5926681 | None:intergenic |
| AGTTCATGTTCTTCCATGCA+TGG | 0.533444 | 1.4:-5926694 | MS.gene57223:CDS |
| AAACACCCACAAAATGGCTT+CGG | 0.536754 | 1.4:-5927247 | None:intergenic |
| CTTTCAGTACTTGTCGAAGC+CGG | 0.537971 | 1.4:+5926964 | None:intergenic |
| AAATACAAACATTTCATCAA+AGG | 0.541877 | 1.4:-5927051 | MS.gene57223:CDS |
| GGTTGCAACATGAAAGGTGA+TGG | 0.558210 | 1.4:-5927030 | MS.gene57223:CDS |
| TATATTGTTGATATTCCACA+TGG | 0.600252 | 1.4:-5926796 | MS.gene57223:CDS |
| TCTTCTTCAGTGTTCCCATG+TGG | 0.603865 | 1.4:+5926781 | None:intergenic |
| ATGAAAGGTGATGGAAACGT+TGG | 0.615437 | 1.4:-5927021 | MS.gene57223:CDS |
| AGCCGGTAAACCCGAAACAA+CGG | 0.615768 | 1.4:+5926981 | None:intergenic |
| AAGCACCATCAAGCATAGTG+TGG | 0.678529 | 1.4:-5927104 | MS.gene57223:CDS |
| ATATTGTTGATATTCCACAT+GGG | 0.707262 | 1.4:-5926795 | MS.gene57223:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAATACAAACATTTCATCAA+AGG | - | chr1.4:5926870-5926889 | MS.gene57223:CDS | 20.0% |
| !!! | ATGAAATGTTTGTATTTTTG+TGG | + | chr1.4:5926867-5926886 | None:intergenic | 20.0% |
| !!! | ATGTGGTATTTTTTTATGAT+TGG | + | chr1.4:5926747-5926766 | None:intergenic | 20.0% |
| !!! | TAAGGTTTATACTATTGTTT+TGG | - | chr1.4:5927097-5927116 | MS.gene57223:CDS | 20.0% |
| ! | ATATTGTTGATATTCCACAT+GGG | - | chr1.4:5927126-5927145 | MS.gene57223:CDS | 25.0% |
| ! | TATATTGTTGATATTCCACA+TGG | - | chr1.4:5927125-5927144 | MS.gene57223:CDS | 25.0% |
| !! | TACTGAAAGATTAGAGATTT+TGG | - | chr1.4:5926971-5926990 | MS.gene57223:CDS | 25.0% |
| !!! | GTAATAAGTTTTAGAGTTGT+TGG | - | chr1.4:5927008-5927027 | MS.gene57223:CDS | 25.0% |
| AAGCTTAATCTTCAGAAACT+TGG | - | chr1.4:5927191-5927210 | MS.gene57223:CDS | 30.0% | |
| AATGAGTTTGTCAACAATGA+AGG | - | chr1.4:5927074-5927093 | MS.gene57223:CDS | 30.0% | |
| TTCTCAAAGCTTCTAACATA+TGG | + | chr1.4:5926843-5926862 | None:intergenic | 30.0% | |
| !!! | ATAAGTTTTAGAGTTGTTGG+TGG | - | chr1.4:5927011-5927030 | MS.gene57223:CDS | 30.0% |
| AAACTATCGTTCGGTTACTT+CGG | - | chr1.4:5927049-5927068 | MS.gene57223:CDS | 35.0% | |
| AACAGTGTCAACAAACATCT+TGG | + | chr1.4:5927168-5927187 | None:intergenic | 35.0% | |
| ATCAAAGGTTGCAACATGAA+AGG | - | chr1.4:5926885-5926904 | MS.gene57223:CDS | 35.0% | |
| GTTTGTCAACAATGAAGGTA+AGG | - | chr1.4:5927079-5927098 | MS.gene57223:CDS | 35.0% | |
| TAGAGAAGTTACCGTTGTTT+CGG | - | chr1.4:5926929-5926948 | MS.gene57223:CDS | 35.0% | |
| TCGTCTTCAAAACTATCGTT+CGG | - | chr1.4:5927040-5927059 | MS.gene57223:CDS | 35.0% | |
| !! | TGATGGTGCTTCTATTTTGT+AGG | + | chr1.4:5926808-5926827 | None:intergenic | 35.0% |
| !! | TTGAGGTTGGTTCTAATATG+TGG | + | chr1.4:5926764-5926783 | None:intergenic | 35.0% |
| AGTTCATGTTCTTCCATGCA+TGG | - | chr1.4:5927227-5927246 | MS.gene57223:CDS | 40.0% | |
| GATTGAAGTGCATGTGTTTG+AGG | + | chr1.4:5926781-5926800 | None:intergenic | 40.0% | |
| ! | AGAGAAGTTACCGTTGTTTC+GGG | - | chr1.4:5926930-5926949 | MS.gene57223:CDS | 40.0% |
| ! | GCTTCGGTGCATAATATTCA+AGG | - | chr1.4:5926690-5926709 | MS.gene57223:CDS | 40.0% |
| !! | ATGAAAGGTGATGGAAACGT+TGG | - | chr1.4:5926900-5926919 | MS.gene57223:CDS | 40.0% |
| AAGCACCATCAAGCATAGTG+TGG | - | chr1.4:5926817-5926836 | MS.gene57223:CDS | 45.0% | |
| CTTTCAGTACTTGTCGAAGC+CGG | + | chr1.4:5926960-5926979 | None:intergenic | 45.0% | |
| GGTTGCAACATGAAAGGTGA+TGG | - | chr1.4:5926891-5926910 | MS.gene57223:CDS | 45.0% | |
| TATGGCCACACTATGCTTGA+TGG | + | chr1.4:5926825-5926844 | None:intergenic | 45.0% | |
| TCTTCTTCAGTGTTCCCATG+TGG | + | chr1.4:5927143-5927162 | None:intergenic | 45.0% | |
| ! | GAAGTGCATGTGTTTGAGGT+TGG | + | chr1.4:5926777-5926796 | None:intergenic | 45.0% |
| !! | TACCGTTGTTTCGGGTTTAC+CGG | - | chr1.4:5926938-5926957 | MS.gene57223:CDS | 45.0% |
| AGCCGGTAAACCCGAAACAA+CGG | + | chr1.4:5926943-5926962 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.4 | gene | 5926687 | 5927256 | 5926687 | ID=MS.gene57223 |
| chr1.4 | mRNA | 5926687 | 5927256 | 5926687 | ID=MS.gene57223.t1;Parent=MS.gene57223 |
| chr1.4 | exon | 5926687 | 5927256 | 5926687 | ID=MS.gene57223.t1.exon1;Parent=MS.gene57223.t1 |
| chr1.4 | CDS | 5926687 | 5927256 | 5926687 | ID=cds.MS.gene57223.t1;Parent=MS.gene57223.t1 |
| Gene Sequence |
| Protein sequence |
