Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene069057.t1 | RHN51538.1 | 50 | 54 | 26 | 1 | 10 | 62 | 52 | 105 | 9.50E-06 | 58.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene069057.t1 | A0A396HG13 | 50.0 | 54 | 26 | 1 | 10 | 62 | 52 | 105 | 6.9e-06 | 58.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049262 | MS.gene069057 | 0.810749 | 9.87E-51 | -1.69E-46 |
| MS.gene052534 | MS.gene069057 | 0.80923 | 2.10E-50 | -1.69E-46 |
| MS.gene056854 | MS.gene069057 | 0.812829 | 3.48E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 15 sgRNAs with CRISPR-Local
Find 42 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATGCGGATCAAACCTTGTTT+TGG | 0.270905 | 7.2:-34540539 | MS.gene069057:CDS |
| GTGTTAGGTCTCTGTGCTCT+TGG | 0.339752 | 7.2:-34540438 | MS.gene069057:CDS |
| TTTGGGATGATGTGTGGGTG+GGG | 0.364785 | 7.2:-34540521 | MS.gene069057:CDS |
| AAAGATTGTTGGGAGAGATA+TGG | 0.434200 | 7.2:+34540463 | None:intergenic |
| ATAGCAGCATTGAAAAGTTA+TGG | 0.444339 | 7.2:-34540872 | MS.gene069057:CDS |
| TGTTAGGTCTCTGTGCTCTT+GGG | 0.461624 | 7.2:-34540437 | MS.gene069057:CDS |
| GGTGGGGAATGCCTCGTTGA+GGG | 0.461737 | 7.2:-34540505 | MS.gene069057:CDS |
| GGAGAGATATGGAAGAGTCT+AGG | 0.485313 | 7.2:+34540474 | None:intergenic |
| AGGTCTCTGTGCTCTTGGGT+AGG | 0.544595 | 7.2:-34540433 | None:intergenic |
| AAAGTTATGGATAGGCACCC+AGG | 0.567881 | 7.2:-34540859 | MS.gene069057:intron |
| GGGTGGGGAATGCCTCGTTG+AGG | 0.572836 | 7.2:-34540506 | MS.gene069057:CDS |
| AGCATTGAAAAGTTATGGAT+AGG | 0.602859 | 7.2:-34540867 | MS.gene069057:CDS |
| CCACACATCATCCCAAAACA+AGG | 0.610526 | 7.2:+34540527 | None:intergenic |
| GACTAGAAAGACTAGAGATG+CGG | 0.639356 | 7.2:-34540556 | MS.gene069057:CDS |
| AGGAAATCGCTCCCTCAACG+AGG | 0.742893 | 7.2:+34540494 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTTCAACTATTTATTTGAAA+TGG | + | chr7.2:34540509-34540528 | None:intergenic | 15.0% |
| !! | GAAATGGTAAAATATGAAAT+TGG | + | chr7.2:34540493-34540512 | None:intergenic | 20.0% |
| !!! | AATAATAGATTGATGACATT+TGG | + | chr7.2:34540631-34540650 | None:intergenic | 20.0% |
| ACCTAACACAAAAAGATTGT+TGG | + | chr7.2:34540864-34540883 | None:intergenic | 30.0% | |
| ATTGGAATAAGACAAATACC+TGG | + | chr7.2:34540475-34540494 | None:intergenic | 30.0% | |
| CCTAACACAAAAAGATTGTT+GGG | + | chr7.2:34540863-34540882 | None:intergenic | 30.0% | |
| TTGATGCATGTAATTGTTGT+TGG | - | chr7.2:34540682-34540701 | MS.gene069057:intron | 30.0% | |
| TTGGAATAAGACAAATACCT+GGG | + | chr7.2:34540474-34540493 | None:intergenic | 30.0% | |
| TTTGTATGAGATACCTATAG+TGG | + | chr7.2:34540608-34540627 | None:intergenic | 30.0% | |
| ! | AGCATTGAAAAGTTATGGAT+AGG | - | chr7.2:34540446-34540465 | MS.gene069057:CDS | 30.0% |
| ! | ATAGCAGCATTGAAAAGTTA+TGG | - | chr7.2:34540441-34540460 | MS.gene069057:CDS | 30.0% |
| AAAGATTGTTGGGAGAGATA+TGG | + | chr7.2:34540853-34540872 | None:intergenic | 35.0% | |
| ! | AACTTTTCAATGCTGCTATC+AGG | + | chr7.2:34540440-34540459 | None:intergenic | 35.0% |
| ! | CCCAACAATCTTTTTGTGTT+AGG | - | chr7.2:34540860-34540879 | MS.gene069057:CDS | 35.0% |
| ! | TCTTAACTGTGATTGGTTCT+CGG | - | chr7.2:34540718-34540737 | MS.gene069057:intron | 35.0% |
| AGGCAGTTCTTAACTGTGAT+TGG | - | chr7.2:34540711-34540730 | MS.gene069057:intron | 40.0% | |
| GACTAGAAAGACTAGAGATG+CGG | - | chr7.2:34540757-34540776 | MS.gene069057:intron | 40.0% | |
| ! | CTTAACTGTGATTGGTTCTC+GGG | - | chr7.2:34540719-34540738 | MS.gene069057:intron | 40.0% |
| !! | ACCTATAGTGGATTACTGAC+AGG | + | chr7.2:34540596-34540615 | None:intergenic | 40.0% |
| !! | ATGCGGATCAAACCTTGTTT+TGG | - | chr7.2:34540774-34540793 | MS.gene069057:intron | 40.0% |
| !! | CCTATAGTGGATTACTGACA+GGG | + | chr7.2:34540595-34540614 | None:intergenic | 40.0% |
| !! | CTATAGTGGATTACTGACAG+GGG | + | chr7.2:34540594-34540613 | None:intergenic | 40.0% |
| !!! | CTTGTTTTGGGATGATGTGT+GGG | - | chr7.2:34540787-34540806 | MS.gene069057:intron | 40.0% |
| !!! | TGCGGATCAAACCTTGTTTT+GGG | - | chr7.2:34540775-34540794 | MS.gene069057:intron | 40.0% |
| AAAGTTATGGATAGGCACCC+AGG | - | chr7.2:34540454-34540473 | MS.gene069057:CDS | 45.0% | |
| CCACACATCATCCCAAAACA+AGG | + | chr7.2:34540789-34540808 | None:intergenic | 45.0% | |
| CCCTGTCAGTAATCCACTAT+AGG | - | chr7.2:34540592-34540611 | MS.gene069057:intron | 45.0% | |
| GGAGAGATATGGAAGAGTCT+AGG | + | chr7.2:34540842-34540861 | None:intergenic | 45.0% | |
| TCACAGTTAAGAACTGCCTC+TGG | + | chr7.2:34540710-34540729 | None:intergenic | 45.0% | |
| TGTTAGGTCTCTGTGCTCTT+GGG | - | chr7.2:34540876-34540895 | MS.gene069057:CDS | 45.0% | |
| ! | CTCTAGTCTTTCTAGTCACC+TGG | + | chr7.2:34540754-34540773 | None:intergenic | 45.0% |
| ! | GTAATTGTTGTTGGAGCCAG+AGG | - | chr7.2:34540691-34540710 | MS.gene069057:intron | 45.0% |
| !!! | CCTTGTTTTGGGATGATGTG+TGG | - | chr7.2:34540786-34540805 | MS.gene069057:intron | 45.0% |
| !!! | TTTTGGGATGATGTGTGGGT+GGG | - | chr7.2:34540791-34540810 | MS.gene069057:intron | 45.0% |
| GTGTTAGGTCTCTGTGCTCT+TGG | - | chr7.2:34540875-34540894 | MS.gene069057:CDS | 50.0% | |
| GTTCTCGGGTGGAATTAACC+AGG | - | chr7.2:34540733-34540752 | MS.gene069057:intron | 50.0% | |
| ! | AACTGTGATTGGTTCTCGGG+TGG | - | chr7.2:34540722-34540741 | MS.gene069057:intron | 50.0% |
| ! | TTTGGGATGATGTGTGGGTG+GGG | - | chr7.2:34540792-34540811 | MS.gene069057:intron | 50.0% |
| !!! | GTTTTGGGATGATGTGTGGG+TGG | - | chr7.2:34540790-34540809 | MS.gene069057:intron | 50.0% |
| AGGAAATCGCTCCCTCAACG+AGG | + | chr7.2:34540822-34540841 | None:intergenic | 55.0% | |
| GGTGGGGAATGCCTCGTTGA+GGG | - | chr7.2:34540808-34540827 | MS.gene069057:intron | 60.0% | |
| GGGTGGGGAATGCCTCGTTG+AGG | - | chr7.2:34540807-34540826 | MS.gene069057:intron | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.2 | gene | 34540434 | 34540901 | 34540434 | ID=MS.gene069057 |
| chr7.2 | mRNA | 34540434 | 34540901 | 34540434 | ID=MS.gene069057.t1;Parent=MS.gene069057 |
| chr7.2 | exon | 34540860 | 34540901 | 34540860 | ID=MS.gene069057.t1.exon1;Parent=MS.gene069057.t1 |
| chr7.2 | CDS | 34540860 | 34540901 | 34540860 | ID=cds.MS.gene069057.t1;Parent=MS.gene069057.t1 |
| chr7.2 | exon | 34540434 | 34540580 | 34540434 | ID=MS.gene069057.t1.exon2;Parent=MS.gene069057.t1 |
| chr7.2 | CDS | 34540434 | 34540580 | 34540434 | ID=cds.MS.gene069057.t1;Parent=MS.gene069057.t1 |
| Gene Sequence |
| Protein sequence |
