AlfalfaGEDB | MS.gene069178

M. sativa cultivar XinJiangDaYe / MS.gene069178

KEGG: -
GO: GO:0003674 GO:0005488 GO:0005515 GO:0046983
PFAM: PF00010.29

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene069178.t1	XP_003612938.1	97.5	325	8	0	1	325	1	325	1.30E-175	625.5

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene069178.t1	Q9LSL1	57.3	262	94	5	72	325	100	351	9.3e-69	261.9

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene069178.t1	A9YWR2	97.5	325	8	0	1	325	1	325	9.6e-176	625.5

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TFs/TRs:

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Gene ID	Type	Classification
MS.gene069178.t1	TF	bHLH

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene054999	MS.gene069178	0.802119	6.53E-49	-1.69E-46
MS.gene05971	MS.gene069178	0.80511	1.56E-49	-1.69E-46

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PPI:

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene069178.t1	MTR_5g030770	97.538	325	8	0	1	325	1	325	0.0	649
MS.gene069178.t1	MTR_5g030770	95.692	325	8	1	1	325	1	319	0.0	630
MS.gene069178.t1	MTR_7g076840	71.096	301	16	6	5	305	2	231	4.34e-127	363
MS.gene069178.t1	MTR_3g111290	51.585	347	136	14	1	325	1	337	7.42e-89	270
MS.gene069178.t1	MTR_3g498695	46.798	203	79	7	142	322	287	482	5.30e-41	149
MS.gene069178.t1	MTR_3g498695	45.500	200	87	4	142	322	287	483	9.97e-41	148
MS.gene069178.t1	MTR_7g083900	44.211	190	85	3	154	322	284	473	1.18e-40	148
MS.gene069178.t1	MTR_7g083900	44.560	193	81	5	154	322	284	474	6.45e-40	146
MS.gene069178.t1	MTR_3g030700	41.361	191	93	6	141	316	184	370	2.02e-34	129
MS.gene069178.t1	MTR_2g081040	39.785	186	93	5	154	320	175	360	3.80e-33	125

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene069178.t1	AT5G65640	48.361	366	133	11	1	325	1	351	4.10e-86	263
MS.gene069178.t1	AT5G10570	47.953	342	121	12	11	325	4	315	6.13e-76	236
MS.gene069178.t1	AT1G12860	46.739	184	83	3	154	322	265	448	1.77e-41	150
MS.gene069178.t1	AT3G26744	45.745	188	83	2	154	322	305	492	1.69e-40	148
MS.gene069178.t1	AT3G26744	45.745	188	83	2	154	322	305	492	1.69e-40	148
MS.gene069178.t1	AT3G26744	45.745	188	83	2	154	322	305	492	1.69e-40	148
MS.gene069178.t1	AT1G12860	48.750	160	67	3	154	298	265	424	2.44e-37	138
MS.gene069178.t1	AT5G65640	41.608	286	114	12	1	247	1	272	2.51e-37	135
MS.gene069178.t1	AT3G26744	47.561	164	67	2	154	298	305	468	1.35e-36	137
MS.gene069178.t1	AT2G16910	35.149	202	94	7	155	321	313	512	4.70e-21	94.4

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Find 66 sgRNAs with CRISPR-Local

Find 110 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATGGAACTTTCTCAACT+TGG	0.469304	5.3:-19868671	MS.gene069178:CDS
AAACCGTTTAGAAATTTCTA+AGG	0.325538	5.3:-19867707	MS.gene069178:CDS
AATGAGTTACTACTCCCTAA+TGG	0.409168	5.3:-19868587	MS.gene069178:CDS
AATGGTAAGAAATTCCCCTA+AGG	0.619045	5.3:-19867665	MS.gene069178:intron
AATTGTCCCTAAGATCAGCA+AGG	0.709185	5.3:-19868138	MS.gene069178:intron
ACAGCCTTCAAAGAATCTTA+TGG	0.411193	5.3:-19868213	MS.gene069178:CDS
ACATGAAAGAGCTTCTAGAA+AGG	0.506584	5.3:-19867777	MS.gene069178:CDS
ACATGGAATACTTTATCCAC+TGG	0.522465	5.3:-19868614	MS.gene069178:CDS
ACTCCTTAGAAATTTCTAAA+CGG	0.465492	5.3:+19867704	None:intergenic
ACTGAGAATCCATCAAGATA+AGG	0.498561	5.3:+19868431	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AACCATGCAAACAATTATTG+AGG	+	chr5.3:19868533-19868552	None:intergenic	30.0%
AAGGAAATAAACCATACCTT+AGG	+	chr5.3:19867940-19867959	None:intergenic	30.0%
AGAAAAAAGCCAAATCCAAA+AGG	-	chr5.3:19867342-19867361	MS.gene069178:intron	30.0%
AGATAAAGCAATCACTATTC+AGG	-	chr5.3:19868638-19868657	MS.gene069178:CDS	30.0%
AGGAAATAAACCATACCTTA+GGG	+	chr5.3:19867939-19867958	None:intergenic	30.0%
CATAAGGACATTCAAATCTA+TGG	+	chr5.3:19867109-19867128	None:intergenic	30.0%
CATAGATTTGAATGTCCTTA+TGG	-	chr5.3:19867107-19867126	MS.gene069178:intron	30.0%
CATATTATTCCCATGTGTTT+AGG	-	chr5.3:19868582-19868601	MS.gene069178:CDS	30.0%
CATTCAAATCTATGGTCTAA+TGG	+	chr5.3:19867101-19867120	None:intergenic	30.0%
GAAAAAAGCCAAATCCAAAA+GGG	-	chr5.3:19867343-19867362	MS.gene069178:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr5.3	gene	19866918	19868690	19866918	ID=MS.gene069178
chr5.3	mRNA	19866918	19868690	19866918	ID=MS.gene069178.t1;Parent=MS.gene069178
chr5.3	exon	19868139	19868690	19868139	ID=MS.gene069178.t1.exon1;Parent=MS.gene069178.t1
chr5.3	CDS	19868139	19868690	19868139	ID=cds.MS.gene069178.t1;Parent=MS.gene069178.t1
chr5.3	exon	19867666	19867836	19867666	ID=MS.gene069178.t1.exon2;Parent=MS.gene069178.t1
chr5.3	CDS	19867666	19867836	19867666	ID=cds.MS.gene069178.t1;Parent=MS.gene069178.t1
chr5.3	exon	19867405	19867572	19867405	ID=MS.gene069178.t1.exon3;Parent=MS.gene069178.t1
chr5.3	CDS	19867405	19867572	19867405	ID=cds.MS.gene069178.t1;Parent=MS.gene069178.t1
chr5.3	exon	19866918	19867004	19866918	ID=MS.gene069178.t1.exon4;Parent=MS.gene069178.t1
chr5.3	CDS	19866918	19867004	19866918	ID=cds.MS.gene069178.t1;Parent=MS.gene069178.t1

Gene Sequence

>MS.gene069178

ATGGAACTTTCTCAACTTGGTTTTCTTGAGGAGCTACTAGTTCCAAGAAAAGACACATGGAATACTTTATCCACTGGTTTGAATGAGTTACTACTCCCTAATGGTTGGACCTTTGACTCATTTGATGAAAATCTTCTCATCAATCCATCTTTAAACCCTTCATTTGCTTCATTCTCAACACCATTAGACCATAGATTTGAATGTCCTTATGGAACTGATGCATCATCATTATCATACCCTTATCTTGATGGATTCTCAGTTCCTGAGTTTGATGATTCAGCACCAGTTTTACCACAACAGGAAAGTATGGAAGAGTTTGGTTTTGTTGGAAGTGAGAACAAGAGGTTTCAAGAGAGTAAAATCAGCTGCAAAGTTGAGGAACAAGTTAGTGAAACTCCAGTTTTTAACATGGGTTTGTGTGGTGAGAAAAAAGCCAAATCCAAAAGGGTTGAAGGACAGCCTTCAAAGAATCTTATGGCTGAAAGAAGGAGAAGAAAGCGTTTGAATGACAGGCTTTCCATGCTTAGGTCAATTGTCCCTAAGATCAGCAAGGTGATTCAATATATATAATGTGTATGTATGTTTGTGTGTGTATGTTTATGTTGTCTGGCTCCGACACTTCAGATTGAAGATATGTTCAGTTATTCATTTTTGTCAAATTATTATCGGTGTCGAGTATTAGTGTTGTGTCTGGTGTTTATGTAAGTGCTCATAGTGTGTATATGTATGTGTGTTTTATTTTTGGTACAAATGTTTGTGTTTTTTACCTTCATTGACTACAATTGACTAGCATTTGTATTTGGTGTTTATGTAAGTGCTCATTTGAACTAACAAGAAAATGTAAATTTTGTAAGATGGACAGGACATCAATTCTAGGAGATACAATTGATTACATGAAAGAGCTTCTAGAAAGGATTAGTAAGTTACAAGAAGAAATGGAGGAAGAAGGAACAAATCAGATAAACCGTTTAGAAATTTCTAAGGAGTTAAAGCCAAATGAAGTAATGGTAAGAAATTCCCCTAAGGTATGGTTTATTTCCTTGAACAACTAATGCATAATCATAGACCAAGTTTATGAATAAATGTAATGAATGGAATGTTTTCTTTTTCTTTTTCAGTTTGATGTTGAGAGGAGAGACCAGGACACAAGAATCAGTATCTGCTGTGCCACAAAGCCAGGATTGTTACTGTCAACAGTGAACACATTAGAAGCATTAGGCCTTGAGATTCACCAGTGTGTTATAAGCAGTTTCAATGATTTTTCATTGCAAGCATCTTGCTCAGAGGTTAGTAAAACCCTAAGCATGTTCGGATTGATAGCGAATTTGTCAGAATTACATTGTGTCGCCGTCATTTTATTAAAAACTACGCTTTTGAGCTTTAAGGGTTATAATTGATCATGAAAAGTTTGGATGTTTGCGAGTAGAATTGCTTTTGTCTTCAAAATGATTCTAATTTGAAGTTAGAATTTAGCGTTTTTTTTTTCCTCTATAATTAATTTTTCGCCAGGAATTTATTACATTCGATTCAATTTTAGTTGGAAACAATTTTCTTGAATCAATTAATTCAAGATCAATTTTTGTGACCGCAGAACCAAAAAAAAGACTATTCGCCTCAATAATTGTTTGCATGGTTAAAATAAAACATGTTCATACTTCTTTTAACATATTATTCCCATGTGTTTAGGTAGATGGTCAGAGAAATTGTATGAACCCTGAAGAGATAAAGCAATCACTATTCAGGAATGCAGGTTATGGTGGGAGGTGTCTCTAG

Protein sequence

>MS.gene069178.t1

MELSQLGFLEELLVPRKDTWNTLSTGLNELLLPNGWTFDSFDENLLINPSLNPSFASFSTPLDHRFECPYGTDASSLSYPYLDGFSVPEFDDSAPVLPQQESMEEFGFVGSENKRFQESKISCKVEEQVSETPVFNMGLCGEKKAKSKRVEGQPSKNLMAERRRRKRLNDRLSMLRSIVPKISKMDRTSILGDTIDYMKELLERISKLQEEMEEEGTNQINRLEISKELKPNEVMVRNSPKFDVERRDQDTRISICCATKPGLLLSTVNTLEALGLEIHQCVISSFNDFSLQASCSEVDGQRNCMNPEEIKQSLFRNAGYGGRCL