Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene069445.t1 | XP_003620175.1 | 94.4 | 214 | 11 | 1 | 1 | 214 | 1 | 213 | 7.30E-114 | 419.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene069445.t1 | P13087 | 44.2 | 215 | 106 | 5 | 6 | 213 | 9 | 216 | 1.9e-41 | 170.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene069445.t1 | Q6ISX8 | 94.4 | 214 | 11 | 1 | 1 | 214 | 1 | 213 | 5.3e-114 | 419.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene05227 | MS.gene069445 | 0.865408 | 6.03E-65 | -1.69E-46 |
| MS.gene05228 | MS.gene069445 | 0.909486 | 5.40E-82 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene069445.t1 | MTR_6g078140 | 94.393 | 214 | 11 | 1 | 1 | 214 | 1 | 213 | 7.67e-136 | 379 |
| MS.gene069445.t1 | MTR_6g078120 | 93.925 | 214 | 12 | 1 | 1 | 214 | 1 | 213 | 8.85e-135 | 377 |
| MS.gene069445.t1 | MTR_6g078280 | 62.000 | 200 | 69 | 4 | 18 | 214 | 17 | 212 | 6.96e-83 | 245 |
| MS.gene069445.t1 | MTR_6g078290 | 58.605 | 215 | 85 | 4 | 1 | 214 | 1 | 212 | 4.84e-81 | 241 |
| MS.gene069445.t1 | MTR_6g078250 | 56.633 | 196 | 76 | 2 | 16 | 211 | 15 | 201 | 5.39e-73 | 220 |
| MS.gene069445.t1 | MTR_6g078260 | 48.165 | 218 | 90 | 6 | 1 | 214 | 1 | 199 | 2.52e-58 | 182 |
| MS.gene069445.t1 | MTR_7g037410 | 48.663 | 187 | 84 | 4 | 29 | 214 | 27 | 202 | 5.40e-56 | 177 |
| MS.gene069445.t1 | MTR_6g078070 | 40.359 | 223 | 104 | 10 | 1 | 214 | 1 | 203 | 6.93e-40 | 135 |
| MS.gene069445.t1 | MTR_6g078100 | 40.265 | 226 | 102 | 11 | 1 | 214 | 1 | 205 | 9.77e-39 | 133 |
| MS.gene069445.t1 | MTR_6g065460 | 31.019 | 216 | 135 | 5 | 1 | 214 | 1 | 204 | 9.27e-21 | 86.3 |
| MS.gene069445.t1 | MTR_3g106620 | 30.000 | 220 | 122 | 7 | 6 | 214 | 1 | 199 | 2.21e-20 | 85.5 |
| MS.gene069445.t1 | MTR_3g106585 | 31.839 | 223 | 112 | 9 | 6 | 214 | 1 | 197 | 5.63e-19 | 81.6 |
| MS.gene069445.t1 | MTR_2g073010 | 32.589 | 224 | 115 | 11 | 1 | 214 | 1 | 198 | 3.46e-16 | 73.9 |
| MS.gene069445.t1 | MTR_8g461050 | 29.851 | 201 | 119 | 8 | 20 | 213 | 16 | 201 | 3.86e-15 | 71.2 |
| MS.gene069445.t1 | MTR_6g017225 | 32.530 | 166 | 96 | 6 | 10 | 174 | 12 | 162 | 1.32e-14 | 72.0 |
| MS.gene069445.t1 | MTR_6g059410 | 29.412 | 221 | 122 | 11 | 1 | 214 | 1 | 194 | 3.11e-13 | 66.2 |
| MS.gene069445.t1 | MTR_8g059790 | 28.947 | 190 | 113 | 8 | 31 | 213 | 29 | 203 | 9.12e-13 | 65.1 |
| MS.gene069445.t1 | MTR_6g059530 | 29.412 | 221 | 123 | 10 | 1 | 214 | 1 | 195 | 9.74e-13 | 64.7 |
| MS.gene069445.t1 | MTR_6g009650 | 31.707 | 205 | 114 | 9 | 6 | 205 | 9 | 192 | 1.98e-12 | 64.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene069445.t1 | AT1G17860 | 43.119 | 218 | 97 | 8 | 1 | 214 | 1 | 195 | 4.39e-48 | 156 |
| MS.gene069445.t1 | AT1G73260 | 37.129 | 202 | 105 | 7 | 21 | 214 | 18 | 205 | 1.82e-29 | 109 |
| MS.gene069445.t1 | AT1G73325 | 34.091 | 220 | 122 | 9 | 1 | 214 | 1 | 203 | 1.25e-26 | 102 |
| MS.gene069445.t1 | AT1G73330 | 27.979 | 193 | 108 | 8 | 28 | 214 | 25 | 192 | 1.80e-11 | 61.6 |
Find 46 sgRNAs with CRISPR-Local
Find 55 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AATCTTGAACCAATTATTAA+TGG | 0.190104 | 6.4:-52744759 | None:intergenic |
| TTGAGCACACAACCACTTCT+TGG | 0.247520 | 6.4:+52744338 | MS.gene069445:CDS |
| GATGGGAAACCATTAATAAT+TGG | 0.274442 | 6.4:+52744750 | MS.gene069445:CDS |
| GGAGTGTTTGTTGATGAAAA+TGG | 0.277827 | 6.4:+52744860 | MS.gene069445:CDS |
| CACCTGTGAATGGGTATAAC+CGG | 0.336556 | 6.4:-52744440 | None:intergenic |
| AGACAATGGTTTATTACTAT+TGG | 0.339490 | 6.4:+52744707 | MS.gene069445:CDS |
| TGTTGAAATTTAACTTTAAG+AGG | 0.341900 | 6.4:-52744911 | None:intergenic |
| ATTCACAGGTGTGGACCTTA+TGG | 0.370571 | 6.4:+52744452 | MS.gene069445:CDS |
| TGAAAATGGGAATAAACGTT+TGG | 0.383026 | 6.4:+52744874 | MS.gene069445:CDS |
| TGGTTCAAGATTGAGAAGTA+TGG | 0.396705 | 6.4:+52744770 | MS.gene069445:CDS |
| TGGTGGTGTTGTTGGAAATC+CGG | 0.404788 | 6.4:+52744727 | MS.gene069445:CDS |
| GAGTGTTTGTTGATGAAAAT+GGG | 0.410318 | 6.4:+52744861 | MS.gene069445:CDS |
| ATTACTATTGGTGGTGTTGT+TGG | 0.423355 | 6.4:+52744719 | MS.gene069445:CDS |
| GAACAAGTGGTTGACACATT+AGG | 0.434007 | 6.4:+52744380 | MS.gene069445:CDS |
| CTACTTCTACATTTACCATA+AGG | 0.458135 | 6.4:-52744467 | None:intergenic |
| AGTCTTGCCCTAGCAAGTAT+TGG | 0.463122 | 6.4:+52744497 | MS.gene069445:CDS |
| AACTTGCCCTCTTGATGTTG+TGG | 0.473079 | 6.4:+52744523 | MS.gene069445:CDS |
| CAATGGTTTATTACTATTGG+TGG | 0.475905 | 6.4:+52744710 | MS.gene069445:CDS |
| TTATTAATGGTTTCCCATCC+CGG | 0.501459 | 6.4:-52744746 | None:intergenic |
| TTCAAAGACTGCACCACACT+AGG | 0.506900 | 6.4:-52744818 | None:intergenic |
| AAGCATATGTGTAAGGATGT+TGG | 0.510678 | 6.4:+52744839 | MS.gene069445:CDS |
| ATAAGGTCCACACCTGTGAA+TGG | 0.514083 | 6.4:-52744450 | None:intergenic |
| GTCTTTGAAGCATATGTGTA+AGG | 0.517598 | 6.4:+52744832 | MS.gene069445:CDS |
| TAAGGTCCACACCTGTGAAT+GGG | 0.519817 | 6.4:-52744449 | None:intergenic |
| TTAACGGGTGTAAAGGTTAG+TGG | 0.525966 | 6.4:-52744569 | None:intergenic |
| TGCTAATTACTATATTATCC+CGG | 0.540548 | 6.4:+52744421 | MS.gene069445:CDS |
| GGTGGTGTTGTTGGAAATCC+GGG | 0.542963 | 6.4:+52744728 | MS.gene069445:CDS |
| GCTTCAGCTGCTCCAAGAAG+TGG | 0.551993 | 6.4:-52744350 | None:intergenic |
| TCCCGGTTATACCCATTCAC+AGG | 0.554778 | 6.4:+52744438 | MS.gene069445:CDS |
| GTGTTGTTGGAAATCCGGGA+TGG | 0.564391 | 6.4:+52744732 | MS.gene069445:CDS |
| TGTTGTTGGAAATCCGGGAT+GGG | 0.569448 | 6.4:+52744733 | MS.gene069445:CDS |
| TACTTGTCTACACCATTCCA+TGG | 0.570760 | 6.4:+52744652 | MS.gene069445:CDS |
| TCAAAGACTGCACCACACTA+GGG | 0.579576 | 6.4:-52744817 | None:intergenic |
| TATGGTAAATGTAGAAGTAG+TGG | 0.581114 | 6.4:+52744470 | MS.gene069445:CDS |
| CAAGCTTCCACACCATGGAA+TGG | 0.584005 | 6.4:-52744664 | None:intergenic |
| TGAAGCTTCAAATGAACAAG+TGG | 0.587694 | 6.4:+52744367 | MS.gene069445:CDS |
| AAGTGGTTGTGTGCTCAAGA+AGG | 0.588756 | 6.4:-52744333 | None:intergenic |
| GTCTACACCATTCCATGGTG+TGG | 0.596900 | 6.4:+52744657 | MS.gene069445:CDS |
| ACCTGTGAATGGGTATAACC+GGG | 0.598899 | 6.4:-52744439 | None:intergenic |
| TTAATGTATCTAAGAGACAA+TGG | 0.603832 | 6.4:+52744693 | MS.gene069445:CDS |
| GTATGGTGATGCTTATAAGT+TGG | 0.604702 | 6.4:+52744787 | MS.gene069445:CDS |
| TCAACAACCACAACATCAAG+AGG | 0.610607 | 6.4:-52744530 | None:intergenic |
| TATAGTAATTAGCATCAGCT+CGG | 0.621190 | 6.4:-52744412 | None:intergenic |
| GTTATACCCATTCACAGGTG+TGG | 0.621916 | 6.4:+52744443 | MS.gene069445:CDS |
| ACGATCAAGCTTCCACACCA+TGG | 0.629046 | 6.4:-52744669 | None:intergenic |
| CAACAACCACAACATCAAGA+GGG | 0.678487 | 6.4:-52744529 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATCTTGAACCAATTATTAA+TGG | - | chr6.4:52744762-52744781 | None:intergenic | 20.0% |
| !!! | TGTTGAAATTTAACTTTAAG+AGG | - | chr6.4:52744914-52744933 | None:intergenic | 20.0% |
| ! | AGACAATGGTTTATTACTAT+TGG | + | chr6.4:52744707-52744726 | MS.gene069445:CDS | 25.0% |
| ! | TGCTAATTACTATATTATCC+CGG | + | chr6.4:52744421-52744440 | MS.gene069445:CDS | 25.0% |
| ! | TTAATGTATCTAAGAGACAA+TGG | + | chr6.4:52744693-52744712 | MS.gene069445:CDS | 25.0% |
| CAATGGTTTATTACTATTGG+TGG | + | chr6.4:52744710-52744729 | MS.gene069445:CDS | 30.0% | |
| CTACTTCTACATTTACCATA+AGG | - | chr6.4:52744470-52744489 | None:intergenic | 30.0% | |
| GATGGGAAACCATTAATAAT+TGG | + | chr6.4:52744750-52744769 | MS.gene069445:CDS | 30.0% | |
| TATAGTAATTAGCATCAGCT+CGG | - | chr6.4:52744415-52744434 | None:intergenic | 30.0% | |
| TATGGTAAATGTAGAAGTAG+TGG | + | chr6.4:52744470-52744489 | MS.gene069445:CDS | 30.0% | |
| TGAAAATGGGAATAAACGTT+TGG | + | chr6.4:52744874-52744893 | MS.gene069445:CDS | 30.0% | |
| ! | AAGTTTTTCCAATACTTGCT+AGG | - | chr6.4:52744508-52744527 | None:intergenic | 30.0% |
| ! | GAGTGTTTGTTGATGAAAAT+GGG | + | chr6.4:52744861-52744880 | MS.gene069445:CDS | 30.0% |
| !! | AGTTTTTCCAATACTTGCTA+GGG | - | chr6.4:52744507-52744526 | None:intergenic | 30.0% |
| !!! | ACACCTTTTTTTGGATTAAC+GGG | - | chr6.4:52744587-52744606 | None:intergenic | 30.0% |
| !!! | GACACCTTTTTTTGGATTAA+CGG | - | chr6.4:52744588-52744607 | None:intergenic | 30.0% |
| AAGCATATGTGTAAGGATGT+TGG | + | chr6.4:52744839-52744858 | MS.gene069445:CDS | 35.0% | |
| ACACCCGTTAATCCAAAAAA+AGG | + | chr6.4:52744581-52744600 | MS.gene069445:CDS | 35.0% | |
| TGAAGCTTCAAATGAACAAG+TGG | + | chr6.4:52744367-52744386 | MS.gene069445:CDS | 35.0% | |
| TGGTTCAAGATTGAGAAGTA+TGG | + | chr6.4:52744770-52744789 | MS.gene069445:CDS | 35.0% | |
| TTATTAATGGTTTCCCATCC+CGG | - | chr6.4:52744749-52744768 | None:intergenic | 35.0% | |
| ! | GGAGTGTTTGTTGATGAAAA+TGG | + | chr6.4:52744860-52744879 | MS.gene069445:CDS | 35.0% |
| ! | GTATGGTGATGCTTATAAGT+TGG | + | chr6.4:52744787-52744806 | MS.gene069445:CDS | 35.0% |
| !! | ATTACTATTGGTGGTGTTGT+TGG | + | chr6.4:52744719-52744738 | MS.gene069445:CDS | 35.0% |
| !! | GTCTTTGAAGCATATGTGTA+AGG | + | chr6.4:52744832-52744851 | MS.gene069445:CDS | 35.0% |
| !! | TTTTGGATTAACGGGTGTAA+AGG | - | chr6.4:52744579-52744598 | None:intergenic | 35.0% |
| !!! | ACACGAATGACACCTTTTTT+TGG | - | chr6.4:52744596-52744615 | None:intergenic | 35.0% |
| CAACAACCACAACATCAAGA+GGG | - | chr6.4:52744532-52744551 | None:intergenic | 40.0% | |
| GAACAAGTGGTTGACACATT+AGG | + | chr6.4:52744380-52744399 | MS.gene069445:CDS | 40.0% | |
| TACTTGTCTACACCATTCCA+TGG | + | chr6.4:52744652-52744671 | MS.gene069445:CDS | 40.0% | |
| TCAACAACCACAACATCAAG+AGG | - | chr6.4:52744533-52744552 | None:intergenic | 40.0% | |
| TGCTCAAGAAGGCAAAAAGA+AGG | - | chr6.4:52744325-52744344 | None:intergenic | 40.0% | |
| ! | TTAACGGGTGTAAAGGTTAG+TGG | - | chr6.4:52744572-52744591 | None:intergenic | 40.0% |
| AACTTGCCCTCTTGATGTTG+TGG | + | chr6.4:52744523-52744542 | MS.gene069445:CDS | 45.0% | |
| AAGTGGTTGTGTGCTCAAGA+AGG | - | chr6.4:52744336-52744355 | None:intergenic | 45.0% | |
| ACCTGTGAATGGGTATAACC+GGG | - | chr6.4:52744442-52744461 | None:intergenic | 45.0% | |
| AGTCTTGCCCTAGCAAGTAT+TGG | + | chr6.4:52744497-52744516 | MS.gene069445:CDS | 45.0% | |
| ATAAGGTCCACACCTGTGAA+TGG | - | chr6.4:52744453-52744472 | None:intergenic | 45.0% | |
| ATTCACAGGTGTGGACCTTA+TGG | + | chr6.4:52744452-52744471 | MS.gene069445:CDS | 45.0% | |
| CACCTGTGAATGGGTATAAC+CGG | - | chr6.4:52744443-52744462 | None:intergenic | 45.0% | |
| GTTATACCCATTCACAGGTG+TGG | + | chr6.4:52744443-52744462 | MS.gene069445:CDS | 45.0% | |
| TAAGGTCCACACCTGTGAAT+GGG | - | chr6.4:52744452-52744471 | None:intergenic | 45.0% | |
| TCAAAGACTGCACCACACTA+GGG | - | chr6.4:52744820-52744839 | None:intergenic | 45.0% | |
| TGTTGTTGGAAATCCGGGAT+GGG | + | chr6.4:52744733-52744752 | MS.gene069445:CDS | 45.0% | |
| TTCAAAGACTGCACCACACT+AGG | - | chr6.4:52744821-52744840 | None:intergenic | 45.0% | |
| TTGAGCACACAACCACTTCT+TGG | + | chr6.4:52744338-52744357 | MS.gene069445:CDS | 45.0% | |
| !! | TGGTGGTGTTGTTGGAAATC+CGG | + | chr6.4:52744727-52744746 | MS.gene069445:CDS | 45.0% |
| ACGATCAAGCTTCCACACCA+TGG | - | chr6.4:52744672-52744691 | None:intergenic | 50.0% | |
| CAAGCTTCCACACCATGGAA+TGG | - | chr6.4:52744667-52744686 | None:intergenic | 50.0% | |
| GTCTACACCATTCCATGGTG+TGG | + | chr6.4:52744657-52744676 | MS.gene069445:CDS | 50.0% | |
| TCCCGGTTATACCCATTCAC+AGG | + | chr6.4:52744438-52744457 | MS.gene069445:CDS | 50.0% | |
| ! | GTGTTGTTGGAAATCCGGGA+TGG | + | chr6.4:52744732-52744751 | MS.gene069445:CDS | 50.0% |
| !! | GGTGGTGTTGTTGGAAATCC+GGG | + | chr6.4:52744728-52744747 | MS.gene069445:CDS | 50.0% |
| !! | GTTGGTGTTTTGCCCTAGTG+TGG | + | chr6.4:52744805-52744824 | MS.gene069445:CDS | 50.0% |
| GCTTCAGCTGCTCCAAGAAG+TGG | - | chr6.4:52744353-52744372 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.4 | gene | 52744296 | 52744940 | 52744296 | ID=MS.gene069445 |
| chr6.4 | mRNA | 52744296 | 52744940 | 52744296 | ID=MS.gene069445.t1;Parent=MS.gene069445 |
| chr6.4 | exon | 52744296 | 52744940 | 52744296 | ID=MS.gene069445.t1.exon1;Parent=MS.gene069445.t1 |
| chr6.4 | CDS | 52744296 | 52744940 | 52744296 | ID=cds.MS.gene069445.t1;Parent=MS.gene069445.t1 |
| Gene Sequence |
| Protein sequence |
