Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene05228.t1 | XP_003620175.1 | 94.9 | 214 | 10 | 1 | 1 | 214 | 1 | 213 | 1.10E-114 | 422.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene05228.t1 | P13087 | 44.2 | 215 | 106 | 5 | 6 | 213 | 9 | 216 | 2.4e-41 | 170.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene05228.t1 | Q6ISX8 | 94.9 | 214 | 10 | 1 | 1 | 214 | 1 | 213 | 8.1e-115 | 422.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene05227 | MS.gene05228 | 0.900897 | 4.62E-78 | -1.69E-46 |
| MS.gene05228 | MS.gene069445 | 0.909486 | 5.40E-82 | -1.69E-46 |
| MS.gene05228 | MS.gene069446 | 0.922861 | 5.67E-89 | -1.69E-46 |
| MS.gene05228 | MS.gene069447 | 0.921068 | 5.74E-88 | -1.69E-46 |
| MS.gene05228 | MS.gene37737 | 0.916335 | 2.01E-85 | -1.69E-46 |
| MS.gene05228 | MS.gene37738 | 0.916364 | 1.95E-85 | -1.69E-46 |
| MS.gene05228 | MS.gene57819 | 0.817073 | 3.98E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene05228.t1 | MTR_6g078140 | 94.860 | 214 | 10 | 1 | 1 | 214 | 1 | 213 | 1.34e-136 | 381 |
| MS.gene05228.t1 | MTR_6g078120 | 94.393 | 214 | 11 | 1 | 1 | 214 | 1 | 213 | 1.95e-135 | 378 |
| MS.gene05228.t1 | MTR_6g078280 | 62.000 | 200 | 69 | 4 | 18 | 214 | 17 | 212 | 1.69e-82 | 244 |
| MS.gene05228.t1 | MTR_6g078290 | 58.605 | 215 | 85 | 4 | 1 | 214 | 1 | 212 | 9.32e-81 | 240 |
| MS.gene05228.t1 | MTR_6g078250 | 56.633 | 196 | 76 | 2 | 16 | 211 | 15 | 201 | 9.20e-73 | 219 |
| MS.gene05228.t1 | MTR_6g078260 | 48.165 | 218 | 90 | 6 | 1 | 214 | 1 | 199 | 3.57e-58 | 182 |
| MS.gene05228.t1 | MTR_7g037410 | 48.663 | 187 | 84 | 4 | 29 | 214 | 27 | 202 | 1.32e-55 | 176 |
| MS.gene05228.t1 | MTR_6g078070 | 40.359 | 223 | 104 | 10 | 1 | 214 | 1 | 203 | 1.04e-39 | 135 |
| MS.gene05228.t1 | MTR_6g078100 | 40.265 | 226 | 102 | 11 | 1 | 214 | 1 | 205 | 1.08e-38 | 132 |
| MS.gene05228.t1 | MTR_6g065460 | 31.019 | 216 | 135 | 5 | 1 | 214 | 1 | 204 | 8.53e-21 | 86.7 |
| MS.gene05228.t1 | MTR_3g106620 | 29.545 | 220 | 123 | 7 | 6 | 214 | 1 | 199 | 4.40e-20 | 84.7 |
| MS.gene05228.t1 | MTR_3g106585 | 31.390 | 223 | 113 | 9 | 6 | 214 | 1 | 197 | 1.11e-18 | 80.9 |
| MS.gene05228.t1 | MTR_2g073010 | 33.036 | 224 | 114 | 11 | 1 | 214 | 1 | 198 | 5.88e-17 | 76.3 |
| MS.gene05228.t1 | MTR_8g461050 | 29.384 | 211 | 127 | 8 | 10 | 213 | 6 | 201 | 6.06e-15 | 70.9 |
| MS.gene05228.t1 | MTR_6g017225 | 32.530 | 166 | 96 | 6 | 10 | 174 | 12 | 162 | 1.73e-14 | 71.6 |
| MS.gene05228.t1 | MTR_6g009563 | 30.698 | 215 | 123 | 9 | 6 | 214 | 9 | 203 | 1.77e-13 | 67.0 |
| MS.gene05228.t1 | MTR_6g059410 | 29.412 | 221 | 122 | 11 | 1 | 214 | 1 | 194 | 7.22e-13 | 65.1 |
| MS.gene05228.t1 | MTR_8g059790 | 28.947 | 190 | 113 | 8 | 31 | 213 | 29 | 203 | 1.29e-12 | 64.7 |
| MS.gene05228.t1 | MTR_6g059530 | 29.412 | 221 | 123 | 10 | 1 | 214 | 1 | 195 | 1.74e-12 | 64.3 |
| MS.gene05228.t1 | MTR_6g009650 | 31.707 | 205 | 114 | 9 | 6 | 205 | 9 | 192 | 2.13e-12 | 63.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene05228.t1 | AT1G17860 | 43.119 | 218 | 97 | 8 | 1 | 214 | 1 | 195 | 9.08e-48 | 155 |
| MS.gene05228.t1 | AT1G73260 | 36.500 | 200 | 109 | 6 | 21 | 214 | 18 | 205 | 2.30e-29 | 109 |
| MS.gene05228.t1 | AT1G73325 | 34.091 | 220 | 122 | 9 | 1 | 214 | 1 | 203 | 1.37e-26 | 102 |
| MS.gene05228.t1 | AT1G73330 | 27.979 | 193 | 108 | 8 | 28 | 214 | 25 | 192 | 1.73e-11 | 61.6 |
Find 46 sgRNAs with CRISPR-Local
Find 55 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AATCTTGAACCAATTATTAA+TGG | 0.190104 | 6.2:-77641317 | None:intergenic |
| TTGAGCACACAACCACTTCT+TGG | 0.247520 | 6.2:+77640896 | MS.gene05228:CDS |
| GATGGGAAACCATTAATAAT+TGG | 0.274442 | 6.2:+77641308 | MS.gene05228:CDS |
| GGAGTGTTTGTTGATGAAAA+TGG | 0.277827 | 6.2:+77641418 | MS.gene05228:CDS |
| CACCTGTGAATGGGTATAAC+CGG | 0.336556 | 6.2:-77640998 | None:intergenic |
| AGACAATGGTTTATTACTAT+TGG | 0.339490 | 6.2:+77641265 | MS.gene05228:CDS |
| TGTTGAAATTTAACTTTAAG+AGG | 0.341900 | 6.2:-77641469 | None:intergenic |
| ATTCACAGGTGTGGACCTTA+TGG | 0.370571 | 6.2:+77641010 | MS.gene05228:CDS |
| TGAAAATGGGAATAAACGTT+TGG | 0.383026 | 6.2:+77641432 | MS.gene05228:CDS |
| TGGTTCAAGATTGAGAAGTA+TGG | 0.396705 | 6.2:+77641328 | MS.gene05228:CDS |
| TGGTGGTGTTGTTGGAAATC+CGG | 0.404788 | 6.2:+77641285 | MS.gene05228:CDS |
| GAGTGTTTGTTGATGAAAAT+GGG | 0.410318 | 6.2:+77641419 | MS.gene05228:CDS |
| ATTACTATTGGTGGTGTTGT+TGG | 0.423355 | 6.2:+77641277 | MS.gene05228:CDS |
| GAACAAGTGGTTGACACATT+AGG | 0.434007 | 6.2:+77640938 | MS.gene05228:CDS |
| CTACTTCTACATTTACCATA+AGG | 0.458135 | 6.2:-77641025 | None:intergenic |
| TTGAAAGACTGCACCACACT+AGG | 0.459638 | 6.2:-77641376 | None:intergenic |
| AGTCTTGCCCTAGCAAGTAT+TGG | 0.463122 | 6.2:+77641055 | MS.gene05228:CDS |
| GTCTTTCAAGCATATGTGTA+AGG | 0.466167 | 6.2:+77641390 | MS.gene05228:CDS |
| AACTTGCCCTCTTGATGTTG+TGG | 0.473079 | 6.2:+77641081 | MS.gene05228:CDS |
| CAATGGTTTATTACTATTGG+TGG | 0.475905 | 6.2:+77641268 | MS.gene05228:CDS |
| TTATTAATGGTTTCCCATCC+CGG | 0.501459 | 6.2:-77641304 | None:intergenic |
| AAGCATATGTGTAAGGATGT+TGG | 0.504444 | 6.2:+77641397 | MS.gene05228:CDS |
| ATAAGGTCCACACCTGTGAA+TGG | 0.514083 | 6.2:-77641008 | None:intergenic |
| TAAGGTCCACACCTGTGAAT+GGG | 0.519817 | 6.2:-77641007 | None:intergenic |
| TTAACGGGTGTAAAGGTTAG+TGG | 0.525966 | 6.2:-77641127 | None:intergenic |
| TGCTAATTACTATATTATCC+CGG | 0.540548 | 6.2:+77640979 | MS.gene05228:CDS |
| GGTGGTGTTGTTGGAAATCC+GGG | 0.542963 | 6.2:+77641286 | MS.gene05228:CDS |
| GCTTCAGCTGCTCCAAGAAG+TGG | 0.551993 | 6.2:-77640908 | None:intergenic |
| TCCCGGTTATACCCATTCAC+AGG | 0.554778 | 6.2:+77640996 | MS.gene05228:CDS |
| GTGTTGTTGGAAATCCGGGA+TGG | 0.564391 | 6.2:+77641290 | MS.gene05228:CDS |
| TGTTGTTGGAAATCCGGGAT+GGG | 0.569448 | 6.2:+77641291 | MS.gene05228:CDS |
| TACTTGTCTACACCATTCCA+TGG | 0.570760 | 6.2:+77641210 | MS.gene05228:CDS |
| TGAAAGACTGCACCACACTA+GGG | 0.577137 | 6.2:-77641375 | None:intergenic |
| TATGGTAAATGTAGAAGTAG+TGG | 0.581114 | 6.2:+77641028 | MS.gene05228:CDS |
| CAAGCTTCCACACCATGGAA+TGG | 0.584005 | 6.2:-77641222 | None:intergenic |
| TGAAGCTTCAAATGAACAAG+TGG | 0.587694 | 6.2:+77640925 | MS.gene05228:CDS |
| AAGTGGTTGTGTGCTCAAGA+AGG | 0.588756 | 6.2:-77640891 | None:intergenic |
| GTCTACACCATTCCATGGTG+TGG | 0.596900 | 6.2:+77641215 | MS.gene05228:CDS |
| ACCTGTGAATGGGTATAACC+GGG | 0.598899 | 6.2:-77640997 | None:intergenic |
| TTAATGTATCTAAGAGACAA+TGG | 0.603832 | 6.2:+77641251 | MS.gene05228:CDS |
| GTATGGTGATGCTTATAAGT+TGG | 0.604702 | 6.2:+77641345 | MS.gene05228:CDS |
| TCAACAACCACAACATCAAG+AGG | 0.610607 | 6.2:-77641088 | None:intergenic |
| TATAGTAATTAGCATCAGCT+CGG | 0.621190 | 6.2:-77640970 | None:intergenic |
| GTTATACCCATTCACAGGTG+TGG | 0.621916 | 6.2:+77641001 | MS.gene05228:CDS |
| ACGATCAAGCTTCCACACCA+TGG | 0.629046 | 6.2:-77641227 | None:intergenic |
| CAACAACCACAACATCAAGA+GGG | 0.678487 | 6.2:-77641087 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATCTTGAACCAATTATTAA+TGG | - | chr6.2:77641320-77641339 | None:intergenic | 20.0% |
| !!! | TGTTGAAATTTAACTTTAAG+AGG | - | chr6.2:77641472-77641491 | None:intergenic | 20.0% |
| ! | AGACAATGGTTTATTACTAT+TGG | + | chr6.2:77641265-77641284 | MS.gene05228:CDS | 25.0% |
| ! | TGCTAATTACTATATTATCC+CGG | + | chr6.2:77640979-77640998 | MS.gene05228:CDS | 25.0% |
| ! | TTAATGTATCTAAGAGACAA+TGG | + | chr6.2:77641251-77641270 | MS.gene05228:CDS | 25.0% |
| CAATGGTTTATTACTATTGG+TGG | + | chr6.2:77641268-77641287 | MS.gene05228:CDS | 30.0% | |
| CTACTTCTACATTTACCATA+AGG | - | chr6.2:77641028-77641047 | None:intergenic | 30.0% | |
| GATGGGAAACCATTAATAAT+TGG | + | chr6.2:77641308-77641327 | MS.gene05228:CDS | 30.0% | |
| TATAGTAATTAGCATCAGCT+CGG | - | chr6.2:77640973-77640992 | None:intergenic | 30.0% | |
| TATGGTAAATGTAGAAGTAG+TGG | + | chr6.2:77641028-77641047 | MS.gene05228:CDS | 30.0% | |
| TGAAAATGGGAATAAACGTT+TGG | + | chr6.2:77641432-77641451 | MS.gene05228:CDS | 30.0% | |
| ! | AAGTTTTTCCAATACTTGCT+AGG | - | chr6.2:77641066-77641085 | None:intergenic | 30.0% |
| ! | GAGTGTTTGTTGATGAAAAT+GGG | + | chr6.2:77641419-77641438 | MS.gene05228:CDS | 30.0% |
| !! | AGTTTTTCCAATACTTGCTA+GGG | - | chr6.2:77641065-77641084 | None:intergenic | 30.0% |
| !!! | ACACCTTTTTTTGGATTAAC+GGG | - | chr6.2:77641145-77641164 | None:intergenic | 30.0% |
| !!! | GACACCTTTTTTTGGATTAA+CGG | - | chr6.2:77641146-77641165 | None:intergenic | 30.0% |
| AAGCATATGTGTAAGGATGT+TGG | + | chr6.2:77641397-77641416 | MS.gene05228:CDS | 35.0% | |
| ACACCCGTTAATCCAAAAAA+AGG | + | chr6.2:77641139-77641158 | MS.gene05228:CDS | 35.0% | |
| GTCTTTCAAGCATATGTGTA+AGG | + | chr6.2:77641390-77641409 | MS.gene05228:CDS | 35.0% | |
| TGAAGCTTCAAATGAACAAG+TGG | + | chr6.2:77640925-77640944 | MS.gene05228:CDS | 35.0% | |
| TGGTTCAAGATTGAGAAGTA+TGG | + | chr6.2:77641328-77641347 | MS.gene05228:CDS | 35.0% | |
| TTATTAATGGTTTCCCATCC+CGG | - | chr6.2:77641307-77641326 | None:intergenic | 35.0% | |
| ! | GGAGTGTTTGTTGATGAAAA+TGG | + | chr6.2:77641418-77641437 | MS.gene05228:CDS | 35.0% |
| ! | GTATGGTGATGCTTATAAGT+TGG | + | chr6.2:77641345-77641364 | MS.gene05228:CDS | 35.0% |
| !! | ATTACTATTGGTGGTGTTGT+TGG | + | chr6.2:77641277-77641296 | MS.gene05228:CDS | 35.0% |
| !! | TTTTGGATTAACGGGTGTAA+AGG | - | chr6.2:77641137-77641156 | None:intergenic | 35.0% |
| !!! | ACACGAATGACACCTTTTTT+TGG | - | chr6.2:77641154-77641173 | None:intergenic | 35.0% |
| CAACAACCACAACATCAAGA+GGG | - | chr6.2:77641090-77641109 | None:intergenic | 40.0% | |
| GAACAAGTGGTTGACACATT+AGG | + | chr6.2:77640938-77640957 | MS.gene05228:CDS | 40.0% | |
| TACTTGTCTACACCATTCCA+TGG | + | chr6.2:77641210-77641229 | MS.gene05228:CDS | 40.0% | |
| TCAACAACCACAACATCAAG+AGG | - | chr6.2:77641091-77641110 | None:intergenic | 40.0% | |
| TGCTCAAGAAGGCAAAAAGA+AGG | - | chr6.2:77640883-77640902 | None:intergenic | 40.0% | |
| ! | TTAACGGGTGTAAAGGTTAG+TGG | - | chr6.2:77641130-77641149 | None:intergenic | 40.0% |
| AACTTGCCCTCTTGATGTTG+TGG | + | chr6.2:77641081-77641100 | MS.gene05228:CDS | 45.0% | |
| AAGTGGTTGTGTGCTCAAGA+AGG | - | chr6.2:77640894-77640913 | None:intergenic | 45.0% | |
| ACCTGTGAATGGGTATAACC+GGG | - | chr6.2:77641000-77641019 | None:intergenic | 45.0% | |
| AGTCTTGCCCTAGCAAGTAT+TGG | + | chr6.2:77641055-77641074 | MS.gene05228:CDS | 45.0% | |
| ATAAGGTCCACACCTGTGAA+TGG | - | chr6.2:77641011-77641030 | None:intergenic | 45.0% | |
| ATTCACAGGTGTGGACCTTA+TGG | + | chr6.2:77641010-77641029 | MS.gene05228:CDS | 45.0% | |
| CACCTGTGAATGGGTATAAC+CGG | - | chr6.2:77641001-77641020 | None:intergenic | 45.0% | |
| GTTATACCCATTCACAGGTG+TGG | + | chr6.2:77641001-77641020 | MS.gene05228:CDS | 45.0% | |
| TAAGGTCCACACCTGTGAAT+GGG | - | chr6.2:77641010-77641029 | None:intergenic | 45.0% | |
| TGAAAGACTGCACCACACTA+GGG | - | chr6.2:77641378-77641397 | None:intergenic | 45.0% | |
| TGTTGTTGGAAATCCGGGAT+GGG | + | chr6.2:77641291-77641310 | MS.gene05228:CDS | 45.0% | |
| TTGAAAGACTGCACCACACT+AGG | - | chr6.2:77641379-77641398 | None:intergenic | 45.0% | |
| TTGAGCACACAACCACTTCT+TGG | + | chr6.2:77640896-77640915 | MS.gene05228:CDS | 45.0% | |
| !! | TGGTGGTGTTGTTGGAAATC+CGG | + | chr6.2:77641285-77641304 | MS.gene05228:CDS | 45.0% |
| ACGATCAAGCTTCCACACCA+TGG | - | chr6.2:77641230-77641249 | None:intergenic | 50.0% | |
| CAAGCTTCCACACCATGGAA+TGG | - | chr6.2:77641225-77641244 | None:intergenic | 50.0% | |
| GTCTACACCATTCCATGGTG+TGG | + | chr6.2:77641215-77641234 | MS.gene05228:CDS | 50.0% | |
| TCCCGGTTATACCCATTCAC+AGG | + | chr6.2:77640996-77641015 | MS.gene05228:CDS | 50.0% | |
| ! | GTGTTGTTGGAAATCCGGGA+TGG | + | chr6.2:77641290-77641309 | MS.gene05228:CDS | 50.0% |
| !! | GGTGGTGTTGTTGGAAATCC+GGG | + | chr6.2:77641286-77641305 | MS.gene05228:CDS | 50.0% |
| !! | GTTGGTGTTTTGCCCTAGTG+TGG | + | chr6.2:77641363-77641382 | MS.gene05228:CDS | 50.0% |
| GCTTCAGCTGCTCCAAGAAG+TGG | - | chr6.2:77640911-77640930 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.2 | gene | 77640854 | 77641498 | 77640854 | ID=MS.gene05228 |
| chr6.2 | mRNA | 77640854 | 77641498 | 77640854 | ID=MS.gene05228.t1;Parent=MS.gene05228 |
| chr6.2 | exon | 77640854 | 77641498 | 77640854 | ID=MS.gene05228.t1.exon1;Parent=MS.gene05228.t1 |
| chr6.2 | CDS | 77640854 | 77641498 | 77640854 | ID=cds.MS.gene05228.t1;Parent=MS.gene05228.t1 |
| Gene Sequence |
| Protein sequence |
