Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene069447.t1 | XP_003620175.1 | 95.8 | 214 | 8 | 1 | 1 | 214 | 1 | 213 | 3.90E-115 | 424.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene069447.t1 | P13087 | 45.1 | 215 | 104 | 5 | 6 | 213 | 9 | 216 | 5.8e-43 | 175.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene069447.t1 | Q6ISX8 | 95.8 | 214 | 8 | 1 | 1 | 214 | 1 | 213 | 2.8e-115 | 424.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene05227 | MS.gene069447 | 0.856776 | 2.56E-62 | -1.69E-46 |
| MS.gene05228 | MS.gene069447 | 0.921068 | 5.74E-88 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene069447.t1 | MTR_6g078140 | 95.794 | 214 | 8 | 1 | 1 | 214 | 1 | 213 | 4.17e-153 | 423 |
| MS.gene069447.t1 | MTR_6g078120 | 95.327 | 214 | 9 | 1 | 1 | 214 | 1 | 213 | 4.24e-135 | 377 |
| MS.gene069447.t1 | MTR_6g078280 | 62.500 | 200 | 68 | 4 | 18 | 214 | 17 | 212 | 3.07e-83 | 246 |
| MS.gene069447.t1 | MTR_6g078290 | 56.744 | 215 | 89 | 4 | 1 | 214 | 1 | 212 | 4.05e-78 | 233 |
| MS.gene069447.t1 | MTR_6g078250 | 56.633 | 196 | 76 | 2 | 16 | 211 | 15 | 201 | 1.54e-72 | 219 |
| MS.gene069447.t1 | MTR_6g078260 | 48.624 | 218 | 89 | 6 | 1 | 214 | 1 | 199 | 7.21e-59 | 184 |
| MS.gene069447.t1 | MTR_7g037410 | 48.128 | 187 | 85 | 4 | 29 | 214 | 27 | 202 | 3.62e-55 | 175 |
| MS.gene069447.t1 | MTR_6g078070 | 40.090 | 222 | 106 | 9 | 1 | 214 | 1 | 203 | 2.08e-39 | 134 |
| MS.gene069447.t1 | MTR_6g078100 | 39.556 | 225 | 105 | 10 | 1 | 214 | 1 | 205 | 6.66e-38 | 130 |
| MS.gene069447.t1 | MTR_6g065460 | 31.019 | 216 | 135 | 5 | 1 | 214 | 1 | 204 | 3.66e-20 | 84.7 |
| MS.gene069447.t1 | MTR_3g106620 | 28.899 | 218 | 123 | 7 | 5 | 214 | 6 | 199 | 1.22e-18 | 80.9 |
| MS.gene069447.t1 | MTR_3g106585 | 30.275 | 218 | 118 | 8 | 5 | 214 | 6 | 197 | 2.77e-18 | 80.1 |
| MS.gene069447.t1 | MTR_8g461050 | 29.952 | 207 | 123 | 8 | 14 | 213 | 10 | 201 | 2.80e-15 | 71.6 |
| MS.gene069447.t1 | MTR_2g073010 | 32.995 | 197 | 103 | 9 | 24 | 214 | 25 | 198 | 3.42e-15 | 71.2 |
| MS.gene069447.t1 | MTR_2g072980 | 31.163 | 215 | 117 | 10 | 4 | 214 | 12 | 199 | 1.64e-13 | 67.0 |
| MS.gene069447.t1 | MTR_8g059790 | 28.947 | 190 | 113 | 8 | 31 | 213 | 29 | 203 | 1.41e-12 | 64.3 |
| MS.gene069447.t1 | MTR_6g017225 | 29.651 | 172 | 105 | 6 | 4 | 174 | 6 | 162 | 2.45e-12 | 65.5 |
| MS.gene069447.t1 | MTR_6g009563 | 29.767 | 215 | 125 | 9 | 6 | 214 | 9 | 203 | 3.01e-12 | 63.5 |
| MS.gene069447.t1 | MTR_6g009650 | 30.693 | 202 | 115 | 8 | 9 | 205 | 11 | 192 | 3.52e-12 | 63.5 |
| MS.gene069447.t1 | MTR_6g059530 | 28.959 | 221 | 124 | 10 | 1 | 214 | 1 | 195 | 8.06e-12 | 62.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene069447.t1 | AT1G17860 | 43.056 | 216 | 97 | 8 | 3 | 214 | 2 | 195 | 2.03e-47 | 155 |
| MS.gene069447.t1 | AT1G73260 | 35.681 | 213 | 112 | 7 | 8 | 214 | 12 | 205 | 1.86e-29 | 109 |
| MS.gene069447.t1 | AT1G73325 | 34.091 | 220 | 122 | 9 | 1 | 214 | 1 | 203 | 6.84e-26 | 100 |
| MS.gene069447.t1 | AT1G73330 | 27.273 | 220 | 126 | 9 | 1 | 214 | 1 | 192 | 3.29e-12 | 63.5 |
Find 48 sgRNAs with CRISPR-Local
Find 56 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AATCTTGAACCAATTATTAA+TGG | 0.190104 | 6.4:-52764809 | None:intergenic |
| CTTGTTGAAATTTAACTTTA+AGG | 0.254449 | 6.4:-52764963 | None:intergenic |
| TTAAGTTCACAACCACTTCT+TGG | 0.258897 | 6.4:+52764388 | MS.gene069447:CDS |
| GATGGGAAACCATTAATAAT+TGG | 0.274442 | 6.4:+52764800 | MS.gene069447:CDS |
| GGAGTGTTTGTTGATGAAAA+TGG | 0.277827 | 6.4:+52764910 | MS.gene069447:CDS |
| CACCTGTGAATGGGTATAAC+CGG | 0.336556 | 6.4:-52764490 | None:intergenic |
| AGACAATGGTTTATTACTAT+TGG | 0.339490 | 6.4:+52764757 | MS.gene069447:CDS |
| TTGTTGAAATTTAACTTTAA+GGG | 0.352360 | 6.4:-52764962 | None:intergenic |
| ATTCACAGGTGTGGACCTTA+TGG | 0.370571 | 6.4:+52764502 | MS.gene069447:CDS |
| TGGTTCAAGATTGAGAAGTA+TGG | 0.396705 | 6.4:+52764820 | MS.gene069447:CDS |
| TGGTGGTGTTGTTGGAAATC+CGG | 0.404788 | 6.4:+52764777 | MS.gene069447:CDS |
| GATCAAGTGGTCGACACATT+AGG | 0.407221 | 6.4:+52764430 | MS.gene069447:CDS |
| GAGTGTTTGTTGATGAAAAT+GGG | 0.410318 | 6.4:+52764911 | MS.gene069447:CDS |
| TGAAAATGGGAATAAGCGTT+TGG | 0.412010 | 6.4:+52764924 | MS.gene069447:CDS |
| ATTACTATTGGTGGTGTTGT+TGG | 0.423355 | 6.4:+52764769 | MS.gene069447:CDS |
| CTACTTCTACATTTACCATA+AGG | 0.458135 | 6.4:-52764517 | None:intergenic |
| TTGAAAGACTGCACCACACT+AGG | 0.459638 | 6.4:-52764868 | None:intergenic |
| AGTCTTGCCCTAGCAAGTAT+TGG | 0.463122 | 6.4:+52764547 | MS.gene069447:CDS |
| GTCTTTCAAGCATATGTGTA+AGG | 0.466167 | 6.4:+52764882 | MS.gene069447:CDS |
| AACTTGCCCTCTTGATGTTG+TGG | 0.473079 | 6.4:+52764573 | MS.gene069447:CDS |
| CAATGGTTTATTACTATTGG+TGG | 0.475905 | 6.4:+52764760 | MS.gene069447:CDS |
| TTATTAATGGTTTCCCATCC+CGG | 0.501459 | 6.4:-52764796 | None:intergenic |
| AAGCATATGTGTAAGGATGT+TGG | 0.504444 | 6.4:+52764889 | MS.gene069447:CDS |
| ATAAGGTCCACACCTGTGAA+TGG | 0.514083 | 6.4:-52764500 | None:intergenic |
| TAAGGTCCACACCTGTGAAT+GGG | 0.519817 | 6.4:-52764499 | None:intergenic |
| TTAACGGGTGTAAAGGTTAG+TGG | 0.525966 | 6.4:-52764619 | None:intergenic |
| AAGTGGTTGTGAACTTAAGA+TGG | 0.528274 | 6.4:-52764383 | None:intergenic |
| TGTTGAAATTTAACTTTAAG+GGG | 0.534245 | 6.4:-52764961 | None:intergenic |
| TGCTAATTACTATATTATCC+CGG | 0.540548 | 6.4:+52764471 | MS.gene069447:CDS |
| GGTGGTGTTGTTGGAAATCC+GGG | 0.542963 | 6.4:+52764778 | MS.gene069447:CDS |
| GCTTCAGCTGCTCCAAGAAG+TGG | 0.551993 | 6.4:-52764400 | None:intergenic |
| TCCCGGTTATACCCATTCAC+AGG | 0.554778 | 6.4:+52764488 | MS.gene069447:CDS |
| GTGTTGTTGGAAATCCGGGA+TGG | 0.564391 | 6.4:+52764782 | MS.gene069447:CDS |
| TGTTGTTGGAAATCCGGGAT+GGG | 0.569448 | 6.4:+52764783 | MS.gene069447:CDS |
| TACTTGTCTACACCATTCCA+TGG | 0.570760 | 6.4:+52764702 | MS.gene069447:CDS |
| TGAAAGACTGCACCACACTA+GGG | 0.577137 | 6.4:-52764867 | None:intergenic |
| TATGGTAAATGTAGAAGTAG+TGG | 0.581114 | 6.4:+52764520 | MS.gene069447:CDS |
| CAAGCTTCCACACCATGGAA+TGG | 0.584005 | 6.4:-52764714 | None:intergenic |
| GTCTACACCATTCCATGGTG+TGG | 0.596900 | 6.4:+52764707 | MS.gene069447:CDS |
| ACCTGTGAATGGGTATAACC+GGG | 0.598899 | 6.4:-52764489 | None:intergenic |
| TTAATGTATCTAAGAGACAA+TGG | 0.603832 | 6.4:+52764743 | MS.gene069447:CDS |
| GTATGGTGATGCTTATAAGT+TGG | 0.604702 | 6.4:+52764837 | MS.gene069447:CDS |
| TGAAGCTTCAAATGATCAAG+TGG | 0.606293 | 6.4:+52764417 | MS.gene069447:CDS |
| TCAACAACCACAACATCAAG+AGG | 0.610607 | 6.4:-52764580 | None:intergenic |
| TATAGTAATTAGCATCAGCT+CGG | 0.621190 | 6.4:-52764462 | None:intergenic |
| GTTATACCCATTCACAGGTG+TGG | 0.621916 | 6.4:+52764493 | MS.gene069447:CDS |
| ACGATCAAGCTTCCACACCA+TGG | 0.629046 | 6.4:-52764719 | None:intergenic |
| CAACAACCACAACATCAAGA+GGG | 0.678487 | 6.4:-52764579 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTGTTGAAATTTAACTTTAA+GGG | - | chr6.4:52764965-52764984 | None:intergenic | 15.0% |
| !! | AATCTTGAACCAATTATTAA+TGG | - | chr6.4:52764812-52764831 | None:intergenic | 20.0% |
| !!! | CTTGTTGAAATTTAACTTTA+AGG | - | chr6.4:52764966-52764985 | None:intergenic | 20.0% |
| !!! | TGTTGAAATTTAACTTTAAG+GGG | - | chr6.4:52764964-52764983 | None:intergenic | 20.0% |
| ! | AGACAATGGTTTATTACTAT+TGG | + | chr6.4:52764757-52764776 | MS.gene069447:CDS | 25.0% |
| ! | TGCTAATTACTATATTATCC+CGG | + | chr6.4:52764471-52764490 | MS.gene069447:CDS | 25.0% |
| ! | TTAATGTATCTAAGAGACAA+TGG | + | chr6.4:52764743-52764762 | MS.gene069447:CDS | 25.0% |
| CAATGGTTTATTACTATTGG+TGG | + | chr6.4:52764760-52764779 | MS.gene069447:CDS | 30.0% | |
| CTACTTCTACATTTACCATA+AGG | - | chr6.4:52764520-52764539 | None:intergenic | 30.0% | |
| GATGGGAAACCATTAATAAT+TGG | + | chr6.4:52764800-52764819 | MS.gene069447:CDS | 30.0% | |
| TATAGTAATTAGCATCAGCT+CGG | - | chr6.4:52764465-52764484 | None:intergenic | 30.0% | |
| TATGGTAAATGTAGAAGTAG+TGG | + | chr6.4:52764520-52764539 | MS.gene069447:CDS | 30.0% | |
| ! | AAGTTTTTCCAATACTTGCT+AGG | - | chr6.4:52764558-52764577 | None:intergenic | 30.0% |
| ! | GAGTGTTTGTTGATGAAAAT+GGG | + | chr6.4:52764911-52764930 | MS.gene069447:CDS | 30.0% |
| !! | AGTTTTTCCAATACTTGCTA+GGG | - | chr6.4:52764557-52764576 | None:intergenic | 30.0% |
| !!! | ACACCTTTTTTTGGATTAAC+GGG | - | chr6.4:52764637-52764656 | None:intergenic | 30.0% |
| !!! | GACACCTTTTTTTGGATTAA+CGG | - | chr6.4:52764638-52764657 | None:intergenic | 30.0% |
| AAGCATATGTGTAAGGATGT+TGG | + | chr6.4:52764889-52764908 | MS.gene069447:CDS | 35.0% | |
| AAGTGGTTGTGAACTTAAGA+TGG | - | chr6.4:52764386-52764405 | None:intergenic | 35.0% | |
| ACACCCGTTAATCCAAAAAA+AGG | + | chr6.4:52764631-52764650 | MS.gene069447:CDS | 35.0% | |
| GTCTTTCAAGCATATGTGTA+AGG | + | chr6.4:52764882-52764901 | MS.gene069447:CDS | 35.0% | |
| TGAAAATGGGAATAAGCGTT+TGG | + | chr6.4:52764924-52764943 | MS.gene069447:CDS | 35.0% | |
| TGAAGCTTCAAATGATCAAG+TGG | + | chr6.4:52764417-52764436 | MS.gene069447:CDS | 35.0% | |
| TGGTTCAAGATTGAGAAGTA+TGG | + | chr6.4:52764820-52764839 | MS.gene069447:CDS | 35.0% | |
| TTAAGTTCACAACCACTTCT+TGG | + | chr6.4:52764388-52764407 | MS.gene069447:CDS | 35.0% | |
| TTATTAATGGTTTCCCATCC+CGG | - | chr6.4:52764799-52764818 | None:intergenic | 35.0% | |
| ! | GGAGTGTTTGTTGATGAAAA+TGG | + | chr6.4:52764910-52764929 | MS.gene069447:CDS | 35.0% |
| ! | GTATGGTGATGCTTATAAGT+TGG | + | chr6.4:52764837-52764856 | MS.gene069447:CDS | 35.0% |
| !! | ATTACTATTGGTGGTGTTGT+TGG | + | chr6.4:52764769-52764788 | MS.gene069447:CDS | 35.0% |
| !! | TTTTGGATTAACGGGTGTAA+AGG | - | chr6.4:52764629-52764648 | None:intergenic | 35.0% |
| !!! | ACACGAATGACACCTTTTTT+TGG | - | chr6.4:52764646-52764665 | None:intergenic | 35.0% |
| CAACAACCACAACATCAAGA+GGG | - | chr6.4:52764582-52764601 | None:intergenic | 40.0% | |
| TACTTGTCTACACCATTCCA+TGG | + | chr6.4:52764702-52764721 | MS.gene069447:CDS | 40.0% | |
| TCAACAACCACAACATCAAG+AGG | - | chr6.4:52764583-52764602 | None:intergenic | 40.0% | |
| ! | TTAACGGGTGTAAAGGTTAG+TGG | - | chr6.4:52764622-52764641 | None:intergenic | 40.0% |
| AACTTGCCCTCTTGATGTTG+TGG | + | chr6.4:52764573-52764592 | MS.gene069447:CDS | 45.0% | |
| ACCTGTGAATGGGTATAACC+GGG | - | chr6.4:52764492-52764511 | None:intergenic | 45.0% | |
| AGTCTTGCCCTAGCAAGTAT+TGG | + | chr6.4:52764547-52764566 | MS.gene069447:CDS | 45.0% | |
| ATAAGGTCCACACCTGTGAA+TGG | - | chr6.4:52764503-52764522 | None:intergenic | 45.0% | |
| ATTCACAGGTGTGGACCTTA+TGG | + | chr6.4:52764502-52764521 | MS.gene069447:CDS | 45.0% | |
| CACCTGTGAATGGGTATAAC+CGG | - | chr6.4:52764493-52764512 | None:intergenic | 45.0% | |
| GATCAAGTGGTCGACACATT+AGG | + | chr6.4:52764430-52764449 | MS.gene069447:CDS | 45.0% | |
| GTTATACCCATTCACAGGTG+TGG | + | chr6.4:52764493-52764512 | MS.gene069447:CDS | 45.0% | |
| TAAGGTCCACACCTGTGAAT+GGG | - | chr6.4:52764502-52764521 | None:intergenic | 45.0% | |
| TGAAAGACTGCACCACACTA+GGG | - | chr6.4:52764870-52764889 | None:intergenic | 45.0% | |
| TGTTGTTGGAAATCCGGGAT+GGG | + | chr6.4:52764783-52764802 | MS.gene069447:CDS | 45.0% | |
| TTGAAAGACTGCACCACACT+AGG | - | chr6.4:52764871-52764890 | None:intergenic | 45.0% | |
| !! | TGGTGGTGTTGTTGGAAATC+CGG | + | chr6.4:52764777-52764796 | MS.gene069447:CDS | 45.0% |
| ACGATCAAGCTTCCACACCA+TGG | - | chr6.4:52764722-52764741 | None:intergenic | 50.0% | |
| CAAGCTTCCACACCATGGAA+TGG | - | chr6.4:52764717-52764736 | None:intergenic | 50.0% | |
| GTCTACACCATTCCATGGTG+TGG | + | chr6.4:52764707-52764726 | MS.gene069447:CDS | 50.0% | |
| TCCCGGTTATACCCATTCAC+AGG | + | chr6.4:52764488-52764507 | MS.gene069447:CDS | 50.0% | |
| ! | GTGTTGTTGGAAATCCGGGA+TGG | + | chr6.4:52764782-52764801 | MS.gene069447:CDS | 50.0% |
| !! | GGTGGTGTTGTTGGAAATCC+GGG | + | chr6.4:52764778-52764797 | MS.gene069447:CDS | 50.0% |
| !! | GTTGGTGTTTTGCCCTAGTG+TGG | + | chr6.4:52764855-52764874 | MS.gene069447:CDS | 50.0% |
| GCTTCAGCTGCTCCAAGAAG+TGG | - | chr6.4:52764403-52764422 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.4 | gene | 52764346 | 52764990 | 52764346 | ID=MS.gene069447 |
| chr6.4 | mRNA | 52764346 | 52764990 | 52764346 | ID=MS.gene069447.t1;Parent=MS.gene069447 |
| chr6.4 | exon | 52764346 | 52764990 | 52764346 | ID=MS.gene069447.t1.exon1;Parent=MS.gene069447.t1 |
| chr6.4 | CDS | 52764346 | 52764990 | 52764346 | ID=cds.MS.gene069447.t1;Parent=MS.gene069447.t1 |
| Gene Sequence |
| Protein sequence |
