Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene069490.t1 | AES76423.2 | 69.4 | 72 | 22 | 0 | 1 | 72 | 964 | 1035 | 1.80E-19 | 105.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene069490.t1 | A0A072UBS0 | 69.4 | 72 | 22 | 0 | 1 | 72 | 1142 | 1213 | 1.3e-19 | 105.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049850 | MS.gene069490 | 0.802343 | 5.87E-49 | -1.69E-46 |
| MS.gene052863 | MS.gene069490 | 0.804863 | 1.76E-49 | -1.69E-46 |
| MS.gene054467 | MS.gene069490 | 0.804785 | 1.83E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene069490.t1 | MTR_6g078480 | 69.444 | 72 | 22 | 0 | 1 | 72 | 1142 | 1213 | 4.82e-27 | 103 |
| MS.gene069490.t1 | MTR_6g078480 | 69.444 | 72 | 22 | 0 | 1 | 72 | 964 | 1035 | 5.71e-27 | 103 |
| MS.gene069490.t1 | MTR_6g079120 | 82.456 | 57 | 10 | 0 | 1 | 57 | 1078 | 1134 | 1.08e-26 | 102 |
| MS.gene069490.t1 | MTR_6g078890 | 85.455 | 55 | 8 | 0 | 1 | 55 | 1093 | 1147 | 1.15e-26 | 102 |
| MS.gene069490.t1 | MTR_6g078930 | 81.034 | 58 | 11 | 0 | 1 | 58 | 1396 | 1453 | 1.42e-26 | 102 |
| MS.gene069490.t1 | MTR_3g006280 | 79.310 | 58 | 12 | 0 | 1 | 58 | 1471 | 1528 | 1.99e-26 | 101 |
| MS.gene069490.t1 | MTR_6g079090 | 77.419 | 62 | 14 | 0 | 1 | 62 | 1223 | 1284 | 7.67e-26 | 100 |
| MS.gene069490.t1 | MTR_1g019550 | 72.131 | 61 | 17 | 0 | 1 | 61 | 1375 | 1435 | 2.87e-25 | 98.2 |
| MS.gene069490.t1 | MTR_1g019550 | 72.131 | 61 | 17 | 0 | 1 | 61 | 1519 | 1579 | 3.27e-25 | 98.2 |
| MS.gene069490.t1 | MTR_1g019550 | 72.131 | 61 | 17 | 0 | 1 | 61 | 1552 | 1612 | 3.50e-25 | 98.2 |
| MS.gene069490.t1 | MTR_0012s0290 | 53.191 | 94 | 30 | 2 | 1 | 94 | 965 | 1044 | 2.05e-23 | 93.2 |
| MS.gene069490.t1 | MTR_0012s0290 | 66.154 | 65 | 19 | 1 | 1 | 65 | 1083 | 1144 | 1.52e-22 | 90.5 |
| MS.gene069490.t1 | MTR_6g079000 | 69.492 | 59 | 17 | 1 | 1 | 58 | 1648 | 1706 | 2.11e-21 | 87.4 |
| MS.gene069490.t1 | MTR_6g079140 | 75.000 | 52 | 13 | 0 | 1 | 52 | 1227 | 1278 | 3.60e-21 | 86.7 |
| MS.gene069490.t1 | MTR_6g078980 | 81.818 | 44 | 8 | 0 | 7 | 50 | 242 | 285 | 2.05e-18 | 77.4 |
| MS.gene069490.t1 | MTR_6g077640 | 75.000 | 40 | 10 | 0 | 1 | 40 | 1207 | 1246 | 3.36e-14 | 66.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 18 sgRNAs with CRISPR-Local
Find 87 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AACCATGCAGTGGTTGATTT+TGG | 0.211880 | 6.4:-53431064 | MS.gene069490:CDS |
| CCTCAAAGGAATGACTAATT+AGG | 0.261604 | 6.4:+53429593 | None:intergenic |
| GGAAAACTTCAACGATAATA+TGG | 0.274791 | 6.4:-53431113 | MS.gene069490:CDS |
| CCAATATTAGCATCTTTCTC+TGG | 0.305610 | 6.4:+53430968 | None:intergenic |
| CCTAATTAGTCATTCCTTTG+AGG | 0.320162 | 6.4:-53429593 | MS.gene069490:CDS |
| CCAGAGAAAGATGCTAATAT+TGG | 0.359363 | 6.4:-53430968 | MS.gene069490:CDS |
| GGATAACAATCTTTCATCAT+TGG | 0.397150 | 6.4:-53429536 | MS.gene069490:CDS |
| CAACGTAGATCAAACTGGTT+TGG | 0.417119 | 6.4:-53429566 | MS.gene069490:CDS |
| ATTAGCATCTTTCTCTGGGT+TGG | 0.441471 | 6.4:+53430973 | None:intergenic |
| TCAAACTGGTTTGGATGAAT+CGG | 0.469296 | 6.4:-53429557 | MS.gene069490:CDS |
| CAATATTAGCATCTTTCTCT+GGG | 0.479229 | 6.4:+53430969 | None:intergenic |
| ATCTACGTTGGTTTCCTCAA+AGG | 0.502573 | 6.4:+53429579 | None:intergenic |
| GAAACCAACGTAGATCAAAC+TGG | 0.504773 | 6.4:-53429571 | MS.gene069490:CDS |
| GGATTACCATTCCAGAAAAG+TGG | 0.504900 | 6.4:-53431043 | MS.gene069490:CDS |
| GAAAGAGAAAAGTAACATGA+AGG | 0.553858 | 6.4:-53431008 | MS.gene069490:CDS |
| CAAACCAGTTTGATCTACGT+TGG | 0.575664 | 6.4:+53429567 | None:intergenic |
| ATCCAAAATCAACCACTGCA+TGG | 0.600726 | 6.4:+53431062 | None:intergenic |
| AAATTCGTGGAACCATGCAG+TGG | 0.704674 | 6.4:-53431074 | MS.gene069490:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATCTATTACTGTTATTTTTT+GGG | - | chr6.4:53430638-53430657 | MS.gene069490:intron | 15.0% |
| !!! | TATCTATTACTGTTATTTTT+TGG | - | chr6.4:53430637-53430656 | MS.gene069490:intron | 15.0% |
| !! | AAATTAACATATCATCAATG+AGG | + | chr6.4:53430359-53430378 | None:intergenic | 20.0% |
| !!! | ATTGTATTTTCTGTATTTGA+CGG | - | chr6.4:53430446-53430465 | MS.gene069490:intron | 20.0% |
| !!! | TAGTATTACATTGATTATGT+TGG | + | chr6.4:53430619-53430638 | None:intergenic | 20.0% |
| ! | AATTAATGCTATCTTAACAG+TGG | - | chr6.4:53430472-53430491 | MS.gene069490:intron | 25.0% |
| ! | ACAATCCATATTTCACAAAT+TGG | + | chr6.4:53429900-53429919 | None:intergenic | 25.0% |
| ! | CTTAAAAAAATGTTACCTCA+GGG | + | chr6.4:53430405-53430424 | None:intergenic | 25.0% |
| ! | GAATTAAAACCAAAACAAGA+TGG | + | chr6.4:53430890-53430909 | None:intergenic | 25.0% |
| ! | GTAATTGCTTTCTTGTTAAT+TGG | - | chr6.4:53431012-53431031 | MS.gene069490:CDS | 25.0% |
| ! | TATGACCAATTTGTGAAATA+TGG | - | chr6.4:53429892-53429911 | MS.gene069490:intron | 25.0% |
| ! | TTGTATCTTCTACAAAATCA+TGG | - | chr6.4:53430052-53430071 | MS.gene069490:intron | 25.0% |
| !! | AAGAAAGGTTTATGACTTTT+TGG | + | chr6.4:53429751-53429770 | None:intergenic | 25.0% |
| !! | AGAAAGGTTTATGACTTTTT+GGG | + | chr6.4:53429750-53429769 | None:intergenic | 25.0% |
| !! | TACTTTTCTCTTTCAATACA+TGG | + | chr6.4:53429638-53429657 | None:intergenic | 25.0% |
| !! | TATTTTATATGCTAAGAGTC+CGG | - | chr6.4:53430537-53430556 | MS.gene069490:intron | 25.0% |
| !! | TTTTCAATTTCTATACCTCT+GGG | + | chr6.4:53430257-53430276 | None:intergenic | 25.0% |
| !!! | AGTGTTTGTTTTTGGAATAA+AGG | - | chr6.4:53430169-53430188 | MS.gene069490:intron | 25.0% |
| !!! | CTGAGGTAACATTTTTTTAA+GGG | - | chr6.4:53430404-53430423 | MS.gene069490:intron | 25.0% |
| !!! | TCTTGTTTTGGTTTTAATTC+TGG | - | chr6.4:53430890-53430909 | MS.gene069490:intron | 25.0% |
| AGAAAGTAGTATACGAGATT+TGG | - | chr6.4:53430815-53430834 | MS.gene069490:intron | 30.0% | |
| AGGTATAGAAATTGAAAAGG+TGG | - | chr6.4:53430259-53430278 | MS.gene069490:intron | 30.0% | |
| ATTCAAGAATGCTACTGTAA+TGG | - | chr6.4:53430683-53430702 | MS.gene069490:intron | 30.0% | |
| CAAAAAATCACCATGTAAAG+CGG | + | chr6.4:53429934-53429953 | None:intergenic | 30.0% | |
| CAATATTAGCATCTTTCTCT+GGG | + | chr6.4:53429686-53429705 | None:intergenic | 30.0% | |
| CCTTAAAAAAATGTTACCTC+AGG | + | chr6.4:53430406-53430425 | None:intergenic | 30.0% | |
| GAAAGAGAAAAGTAACATGA+AGG | - | chr6.4:53429644-53429663 | MS.gene069490:intron | 30.0% | |
| GGAAAACTTCAACGATAATA+TGG | - | chr6.4:53429539-53429558 | MS.gene069490:CDS | 30.0% | |
| GGATAACAATCTTTCATCAT+TGG | - | chr6.4:53431116-53431135 | MS.gene069490:CDS | 30.0% | |
| TTGTGATTAGAGATTCCATA+TGG | - | chr6.4:53430741-53430760 | MS.gene069490:intron | 30.0% | |
| ! | CAGAGGTATAGAAATTGAAA+AGG | - | chr6.4:53430256-53430275 | MS.gene069490:intron | 30.0% |
| ! | CTTTTCAATTTCTATACCTC+TGG | + | chr6.4:53430258-53430277 | None:intergenic | 30.0% |
| ! | TGTTGATAAGAAGATTACCT+CGG | - | chr6.4:53430289-53430308 | MS.gene069490:intron | 30.0% |
| !! | AAGCACTTTTAGAAAATTCG+TGG | - | chr6.4:53429565-53429584 | MS.gene069490:CDS | 30.0% |
| !! | AATATGGATTGTGTCGATTA+TGG | - | chr6.4:53429908-53429927 | MS.gene069490:intron | 30.0% |
| !!! | CCTGAGGTAACATTTTTTTA+AGG | - | chr6.4:53430403-53430422 | MS.gene069490:intron | 30.0% |
| ATGAAGTATATACTTCCCAG+AGG | - | chr6.4:53430239-53430258 | MS.gene069490:intron | 35.0% | |
| CCAAAACACTTAGACTATAG+AGG | - | chr6.4:53430108-53430127 | MS.gene069490:intron | 35.0% | |
| CCAATATTAGCATCTTTCTC+TGG | + | chr6.4:53429687-53429706 | None:intergenic | 35.0% | |
| CCAGAGAAAGATGCTAATAT+TGG | - | chr6.4:53429684-53429703 | MS.gene069490:intron | 35.0% | |
| CCTAATTAGTCATTCCTTTG+AGG | - | chr6.4:53431059-53431078 | MS.gene069490:CDS | 35.0% | |
| CCTCAAAGGAATGACTAATT+AGG | + | chr6.4:53431062-53431081 | None:intergenic | 35.0% | |
| CTAACATCCTACAACATTAC+AGG | + | chr6.4:53429774-53429793 | None:intergenic | 35.0% | |
| CTACAACATTACAGGAAGAA+AGG | + | chr6.4:53429766-53429785 | None:intergenic | 35.0% | |
| CTATCTTAACAGTGGTGTAT+GGG | - | chr6.4:53430480-53430499 | MS.gene069490:intron | 35.0% | |
| CTTTCTTCCTGTAATGTTGT+AGG | - | chr6.4:53429764-53429783 | MS.gene069490:intron | 35.0% | |
| GAATTCGAAATCTAGTCTGA+GGG | - | chr6.4:53430559-53430578 | MS.gene069490:intron | 35.0% | |
| GCTTACATCTACTTACCATA+TGG | + | chr6.4:53430759-53430778 | None:intergenic | 35.0% | |
| TCAGACTAGATTTCGAATTC+CGG | + | chr6.4:53430559-53430578 | None:intergenic | 35.0% | |
| TTCTACAACTTCTAATACCG+AGG | + | chr6.4:53430309-53430328 | None:intergenic | 35.0% | |
| TTTCTTTAATATGCACCCTG+AGG | - | chr6.4:53430387-53430406 | MS.gene069490:intron | 35.0% | |
| ! | AGTCTAAAATTACCAGCACA+TGG | + | chr6.4:53429859-53429878 | None:intergenic | 35.0% |
| ! | CCTCTATAGTCTAAGTGTTT+TGG | + | chr6.4:53430111-53430130 | None:intergenic | 35.0% |
| !! | AGGACTGTAGTGTTTGTTTT+TGG | - | chr6.4:53430161-53430180 | MS.gene069490:intron | 35.0% |
| !! | AGGGATTTCTGAAACTTCAT+TGG | - | chr6.4:53430578-53430597 | MS.gene069490:intron | 35.0% |
| !! | ATACATGGATTCCACTTTTC+TGG | + | chr6.4:53429623-53429642 | None:intergenic | 35.0% |
| !! | ATTTAGCTGCCATCTTGTTT+TGG | - | chr6.4:53430878-53430897 | MS.gene069490:intron | 35.0% |
| !! | TCAAACTGGTTTGGATGAAT+CGG | - | chr6.4:53431095-53431114 | MS.gene069490:CDS | 35.0% |
| !!! | ATTTTTTGGGAACATAGTCG+AGG | - | chr6.4:53430651-53430670 | MS.gene069490:intron | 35.0% |
| !!! | GTCTAAGTGTTTTGGTTTCA+CGG | + | chr6.4:53430103-53430122 | None:intergenic | 35.0% |
| !!! | TTTTTTGGGAACATAGTCGA+GGG | - | chr6.4:53430652-53430671 | MS.gene069490:intron | 35.0% |
| ATCCAAAATCAACCACTGCA+TGG | + | chr6.4:53429593-53429612 | None:intergenic | 40.0% | |
| ATTAGCATCTTTCTCTGGGT+TGG | + | chr6.4:53429682-53429701 | None:intergenic | 40.0% | |
| CAAACCAGTTTGATCTACGT+TGG | + | chr6.4:53431088-53431107 | None:intergenic | 40.0% | |
| CAACGTAGATCAAACTGGTT+TGG | - | chr6.4:53431086-53431105 | MS.gene069490:CDS | 40.0% | |
| GAAACCAACGTAGATCAAAC+TGG | - | chr6.4:53431081-53431100 | MS.gene069490:CDS | 40.0% | |
| GCTATCTTAACAGTGGTGTA+TGG | - | chr6.4:53430479-53430498 | MS.gene069490:intron | 40.0% | |
| GGAATTCGAAATCTAGTCTG+AGG | - | chr6.4:53430558-53430577 | MS.gene069490:intron | 40.0% | |
| GGATTACCATTCCAGAAAAG+TGG | - | chr6.4:53429609-53429628 | MS.gene069490:intron | 40.0% | |
| GTGGTGTATGGGATCTTATT+CGG | - | chr6.4:53430491-53430510 | MS.gene069490:intron | 40.0% | |
| !! | AACCATGCAGTGGTTGATTT+TGG | - | chr6.4:53429588-53429607 | MS.gene069490:CDS | 40.0% |
| !! | ATCTACGTTGGTTTCCTCAA+AGG | + | chr6.4:53431076-53431095 | None:intergenic | 40.0% |
| !! | TCATGGTGTCACCTTTGTTT+CGG | - | chr6.4:53430069-53430088 | MS.gene069490:intron | 40.0% |
| !! | TGGATTCCACTTTTCTGGAA+TGG | + | chr6.4:53429618-53429637 | None:intergenic | 40.0% |
| AAATTCGTGGAACCATGCAG+TGG | - | chr6.4:53429578-53429597 | MS.gene069490:CDS | 45.0% | |
| CATTTGCGCTAGATGAGCAT+AGG | + | chr6.4:53430222-53430241 | None:intergenic | 45.0% | |
| CGCGTAGATTATGCCAAATC+CGG | + | chr6.4:53430032-53430051 | None:intergenic | 45.0% | |
| GCGTAGATTATGCCAAATCC+GGG | + | chr6.4:53430031-53430050 | None:intergenic | 45.0% | |
| GGAACATAGTCGAGGGATTA+TGG | - | chr6.4:53430659-53430678 | MS.gene069490:intron | 45.0% | |
| TAGATTATGCCAAATCCGGG+TGG | + | chr6.4:53430028-53430047 | None:intergenic | 45.0% | |
| TGTAAAGTTGCACCATGTGC+TGG | - | chr6.4:53429844-53429863 | MS.gene069490:intron | 45.0% | |
| ! | CGGTGAGTTTTCCGAAACAA+AGG | + | chr6.4:53430083-53430102 | None:intergenic | 45.0% |
| ! | TCGATTATGGCCGCTTTACA+TGG | - | chr6.4:53429921-53429940 | MS.gene069490:intron | 45.0% |
| !! | AATTTATTATTAAAAAGAAA+TGG | + | chr6.4:53430136-53430155 | None:intergenic | 5.0% |
| !!! | TTTTAATAATAAATTAAGAA+AGG | - | chr6.4:53430141-53430160 | MS.gene069490:intron | 5.0% |
| CTGAATGACGTTGTGCCACC+CGG | - | chr6.4:53430010-53430029 | MS.gene069490:intron | 55.0% | |
| GACGTTGTGCCACCCGGATT+TGG | - | chr6.4:53430016-53430035 | MS.gene069490:intron | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.4 | gene | 53429531 | 53431143 | 53429531 | ID=MS.gene069490 |
| chr6.4 | mRNA | 53429531 | 53431143 | 53429531 | ID=MS.gene069490.t1;Parent=MS.gene069490 |
| chr6.4 | exon | 53430949 | 53431143 | 53430949 | ID=MS.gene069490.t1.exon1;Parent=MS.gene069490.t1 |
| chr6.4 | CDS | 53430949 | 53431143 | 53430949 | ID=cds.MS.gene069490.t1;Parent=MS.gene069490.t1 |
| chr6.4 | exon | 53429531 | 53429626 | 53429531 | ID=MS.gene069490.t1.exon2;Parent=MS.gene069490.t1 |
| chr6.4 | CDS | 53429531 | 53429626 | 53429531 | ID=cds.MS.gene069490.t1;Parent=MS.gene069490.t1 |
| Gene Sequence |
| Protein sequence |
