AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene069584

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene069584.t1 MTR_8g099420 96.510 659 16 1 1 652 1 659 0.0 1315
MS.gene069584.t1 MTR_5g009350 75.988 658 150 2 1 651 1 657 0.0 1074
MS.gene069584.t1 MTR_5g009350 76.117 649 147 2 1 642 1 648 0.0 1058
MS.gene069584.t1 MTR_5g009360 74.618 654 159 1 5 651 4 657 0.0 1052
MS.gene069584.t1 MTR_5g009350 73.975 561 138 2 1 554 1 560 0.0 891
MS.gene069584.t1 MTR_5g009350 75.503 547 126 2 1 540 1 546 0.0 889
MS.gene069584.t1 MTR_5g009360 73.896 521 129 1 138 651 11 531 0.0 826
MS.gene069584.t1 MTR_4g068670 56.508 653 275 3 7 652 5 655 0.0 781
MS.gene069584.t1 MTR_1g034360 56.269 654 277 3 7 652 5 657 0.0 776
MS.gene069584.t1 MTR_7g109830 51.070 654 313 1 5 651 2 655 0.0 689
MS.gene069584.t1 MTR_1g086600 48.636 660 320 3 5 650 24 678 0.0 660
MS.gene069584.t1 MTR_1g104810 39.901 609 350 9 49 649 18 618 1.81e-146 439
MS.gene069584.t1 MTR_1g104810 40.033 612 351 9 46 649 82 685 5.54e-146 440
MS.gene069584.t1 MTR_1g030290 30.488 656 395 12 42 650 55 696 1.46e-91 298
MS.gene069584.t1 MTR_3g082660 30.735 667 411 13 25 648 68 726 1.63e-91 299
MS.gene069584.t1 MTR_3g087980 30.556 648 400 12 42 648 54 692 1.56e-87 288
MS.gene069584.t1 MTR_3g084450 25.224 670 402 23 49 649 81 720 9.95e-41 159
MS.gene069584.t1 MTR_3g084470 24.627 670 409 23 46 649 76 715 7.43e-39 153
MS.gene069584.t1 MTR_8g083170 23.238 667 421 21 49 649 83 724 2.28e-34 139
MS.gene069584.t1 MTR_3g084450 25.726 482 285 15 221 649 51 512 6.50e-34 136
MS.gene069584.t1 MTR_1g063110 25.000 356 205 15 194 534 158 466 6.59e-23 103
MS.gene069584.t1 MTR_5g007640 26.939 490 244 20 78 533 95 504 1.59e-21 99.4
MS.gene069584.t1 MTR_4g057730 29.003 331 174 15 216 534 179 460 1.13e-20 95.9
MS.gene069584.t1 MTR_4g057730 29.003 331 174 15 216 534 179 460 1.35e-20 96.3
MS.gene069584.t1 MTR_3g035130 22.793 487 294 16 41 516 1 416 4.68e-20 94.0
MS.gene069584.t1 MTR_3g035130 22.793 487 294 16 41 516 1 416 6.24e-20 94.0
MS.gene069584.t1 MTR_1g026310 25.382 327 187 11 222 538 203 482 7.93e-20 94.0
MS.gene069584.t1 MTR_4g057730 28.205 312 166 13 216 516 179 443 1.19e-19 92.8
MS.gene069584.t1 MTR_2g095510 27.666 347 183 16 203 534 164 457 1.77e-19 92.8
MS.gene069584.t1 MTR_4g077900 27.566 341 194 13 203 533 98 395 2.27e-19 92.0
MS.gene069584.t1 MTR_1g063120 22.417 571 357 20 69 625 47 545 3.26e-19 92.0
MS.gene069584.t1 MTR_3g064420 22.669 547 299 21 81 582 41 508 4.31e-19 91.7
MS.gene069584.t1 MTR_1g090737 27.299 348 182 15 204 534 191 484 4.87e-19 91.7
MS.gene069584.t1 MTR_4g121600 23.033 521 312 17 44 538 20 477 4.89e-19 91.7
MS.gene069584.t1 MTR_8g039720 24.574 411 241 13 221 625 205 552 5.22e-19 91.3
MS.gene069584.t1 MTR_5g027480 27.457 346 191 13 214 545 185 484 5.78e-19 91.3
MS.gene069584.t1 MTR_2g095510 26.994 326 177 14 203 516 164 440 8.39e-19 90.1
MS.gene069584.t1 MTR_3g460770 26.420 352 203 14 203 543 162 468 1.85e-18 89.7
MS.gene069584.t1 MTR_4g005750 23.029 482 285 15 81 543 54 468 7.24e-18 87.8
MS.gene069584.t1 MTR_2g105570 23.251 486 297 17 65 534 41 466 2.22e-17 86.3
MS.gene069584.t1 MTR_8g074120 25.058 431 239 18 126 531 40 411 9.01e-17 84.3
MS.gene069584.t1 MTR_3g088870 26.361 349 206 11 179 516 151 459 2.77e-15 79.7
MS.gene069584.t1 MTR_5g023730 21.811 541 348 15 66 589 32 514 8.57e-14 74.7
MS.gene069584.t1 MTR_1g073180 31.818 154 100 5 380 531 321 471 8.28e-12 68.6
MS.gene069584.t1 MTR_1g073180 31.818 154 100 5 380 531 299 449 9.28e-12 68.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene069584.t1 AT4G23850 70.320 657 188 1 1 650 1 657 0.0 1002
MS.gene069584.t1 AT4G11030 69.651 659 193 1 1 652 1 659 0.0 991
MS.gene069584.t1 AT1G64400 66.820 654 209 2 7 652 6 659 0.0 942
MS.gene069584.t1 AT1G49430 52.599 654 301 3 8 652 8 661 0.0 727
MS.gene069584.t1 AT2G47240 48.313 652 330 1 5 649 2 653 0.0 649
MS.gene069584.t1 AT2G47240 48.313 652 330 1 5 649 2 653 0.0 649
MS.gene069584.t1 AT2G47240 48.313 652 330 1 5 649 2 653 0.0 649
MS.gene069584.t1 AT2G47240 48.822 594 297 1 63 649 1 594 0.0 597
MS.gene069584.t1 AT1G49430 48.687 495 246 2 8 494 8 502 6.87e-177 513
MS.gene069584.t1 AT5G27600 41.138 615 342 9 44 648 83 687 2.21e-153 459
MS.gene069584.t1 AT3G05970 40.294 613 348 9 46 649 85 688 1.19e-143 434
MS.gene069584.t1 AT2G04350 31.287 684 385 15 25 648 61 719 1.05e-96 313
MS.gene069584.t1 AT2G04350 31.287 684 385 15 25 648 61 719 1.05e-96 313
MS.gene069584.t1 AT1G77590 30.594 657 385 15 42 648 55 690 4.22e-86 284
MS.gene069584.t1 AT1G77590 32.979 564 321 14 121 648 2 544 2.88e-80 265
MS.gene069584.t1 AT3G23790 24.888 671 401 25 49 649 85 722 5.81e-39 154
MS.gene069584.t1 AT4G14070 24.857 700 413 27 18 648 71 726 1.88e-36 146
MS.gene069584.t1 AT4G19010 30.435 322 179 11 218 534 199 480 8.53e-23 103
MS.gene069584.t1 AT1G66120 25.465 538 293 25 51 558 20 479 2.78e-21 98.6
MS.gene069584.t1 AT1G51680 24.853 511 272 18 55 533 48 478 9.32e-21 96.7
MS.gene069584.t1 AT1G51680 24.853 511 272 18 55 533 48 478 1.23e-20 96.7
MS.gene069584.t1 AT1G51680 24.853 511 272 18 55 533 48 478 1.32e-20 96.3
MS.gene069584.t1 AT3G21240 27.327 333 177 13 217 533 188 471 1.43e-20 96.3
MS.gene069584.t1 AT3G21240 27.327 333 177 13 217 533 188 471 1.76e-20 95.5
MS.gene069584.t1 AT3G21240 27.327 333 177 13 217 533 188 471 1.76e-20 95.5
MS.gene069584.t1 AT4G05160 29.070 344 188 16 199 533 165 461 6.67e-20 94.4
MS.gene069584.t1 AT3G21230 27.545 334 191 13 218 543 205 495 1.02e-17 87.4
MS.gene069584.t1 AT3G21230 27.545 334 191 13 218 543 205 495 1.05e-17 87.4
MS.gene069584.t1 AT3G21230 27.545 334 191 13 218 543 205 495 1.47e-17 86.7
MS.gene069584.t1 AT2G47245 41.935 93 54 0 6 98 36 128 1.54e-17 79.7
MS.gene069584.t1 AT1G20480 27.129 317 180 13 222 533 212 482 2.35e-15 80.1
MS.gene069584.t1 AT5G38120 25.234 428 230 19 222 639 198 545 2.02e-13 73.9
MS.gene069584.t1 AT1G62940 27.119 354 199 17 203 543 160 467 2.08e-13 73.6

Find 159 sgRNAs with CRISPR-Local

Find 0 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGGACCTCCTTTACCATATT+TGG 0.156596 4.4:+4900883 MS.gene069584:CDS
AATACAAGTTTGTCCATTAA+TGG 0.197163 4.4:-4900437 None:intergenic
AACATTATTACACTCTTATC+TGG 0.199872 4.4:+4899194 MS.gene069584:CDS
TCCATTCGTGCTTGTGGTTA+TGG 0.243465 4.4:+4896590 MS.gene069584:CDS
TATGACTTGGTGATCAAAAT+TGG 0.249377 4.4:+4896563 MS.gene069584:CDS
AAGAGGAGAAATCTGTATTA+AGG 0.259788 4.4:+4900961 MS.gene069584:CDS
TTGACAGAAAAGATTAATAA+TGG 0.263355 4.4:+4900183 MS.gene069584:CDS
GGAGTAAAATGCGGCATTTA+TGG 0.277750 4.4:+4897393 MS.gene069584:CDS
TCCAAACACACTTCCAAATA+TGG 0.279040 4.4:-4900896 None:intergenic
CAAAGATGGTAGGCCATCTA+TGG 0.282205 4.4:+4895373 MS.gene069584:CDS
GTTCCTTTATATGATACTTT+AGG 0.288914 4.4:+4897892 MS.gene069584:CDS
GATCAGGTAAAACAAGCTTT+AGG 0.291395 4.4:+4900569 MS.gene069584:intron
TCTTCAAGTGCTTTCTTGCT+TGG 0.295970 4.4:-4901517 None:intergenic
AGTGCATCATATCCCATTTC+AGG 0.308365 4.4:-4900926 None:intergenic
TTGTTTCAGATATGGATCTA+TGG 0.308753 4.4:+4901457 MS.gene069584:intron
CTTCAAGTGCTTTCTTGCTT+GGG 0.325012 4.4:-4901516 None:intergenic
TGAAACGGCAGCTCCATTAA+TGG 0.329839 4.4:+4900424 MS.gene069584:CDS
CCATTCGTGCTTGTGGTTAT+GGG 0.333564 4.4:+4896591 MS.gene069584:CDS
GTGGTGCTGCAATAGGTTTC+TGG 0.337284 4.4:+4899680 MS.gene069584:CDS
CATAGATGGCCTACCATCTT+TGG 0.340044 4.4:-4895372 None:intergenic
TTGAGATACTCTGCTGCCTT+TGG 0.342904 4.4:-4898201 None:intergenic
CCAAGCATATTTATTTCGTT+TGG 0.345668 4.4:-4900854 None:intergenic
GCTCATATCTTACAAGGATA+TGG 0.350220 4.4:+4900671 MS.gene069584:CDS
ACAGATTTCTCCTCTTGGTT+TGG 0.353378 4.4:-4900954 None:intergenic
ATCAGGTAAAACAAGCTTTA+GGG 0.354022 4.4:+4900570 MS.gene069584:intron
TGACAGAAAAGATTAATAAT+GGG 0.357421 4.4:+4900184 MS.gene069584:CDS
CGACCCAGCATTGGATTTGT+TGG 0.367544 4.4:-4896399 None:intergenic
ACCATATTTGGAAGTGTGTT+TGG 0.368130 4.4:+4900895 MS.gene069584:CDS
ATAAAGTCCATGAGCATTGC+AGG 0.376947 4.4:-4897866 None:intergenic
TCCATCAATCATAACCTCTT+TGG 0.384969 4.4:-4901026 None:intergenic
GCAAGAAAGCACTTGAAGAT+TGG 0.395350 4.4:+4901521 MS.gene069584:CDS
ACCAAAGAGGTTATGATTGA+TGG 0.395883 4.4:+4901025 MS.gene069584:CDS
CACTTGCACACATCTTTGAC+AGG 0.399229 4.4:+4899632 MS.gene069584:CDS
ATCTATTAAACAGGAGATAT+CGG 0.400047 4.4:+4901130 MS.gene069584:intron
TTTGGAGTCTGTACCTGAAA+TGG 0.402444 4.4:+4900913 MS.gene069584:CDS
AGCAAGAAGTTGAGAAGATC+GGG 0.409532 4.4:+4898354 MS.gene069584:CDS
ATACCTAAAGTATCATATAA+AGG 0.411632 4.4:-4897895 None:intergenic
ATCTCTCCTCCTCCCATTGA+AGG 0.413564 4.4:+4895428 MS.gene069584:CDS
CATTTATGGTGCCAATTGTC+CGG 0.414011 4.4:+4897407 MS.gene069584:CDS
ATATGGAGTGGTGCTGCAAT+AGG 0.415085 4.4:+4899673 MS.gene069584:CDS
ATCGGAGAGTGGCAACCTAA+TGG 0.419480 4.4:+4901148 MS.gene069584:CDS
TGTGTAGCAATTGTGAGCTT+TGG 0.422578 4.4:+4898311 MS.gene069584:intron
CATAATCCATTCCGGACAAT+TGG 0.424577 4.4:-4897418 None:intergenic
CAAGAAAGCACTTGAAGATT+GGG 0.431537 4.4:+4901522 MS.gene069584:CDS
AAGGCGAAACATTACATACT+TGG 0.431539 4.4:+4901595 MS.gene069584:CDS
CGTTCCATGTCAAATGGAAC+TGG 0.435440 4.4:-4901799 None:intergenic
TCCATGCTCATAATCCATTC+CGG 0.452071 4.4:-4897426 None:intergenic
AGAGGAGAAATCTGTATTAA+GGG 0.455958 4.4:+4900962 MS.gene069584:CDS
CTGCAATAGGTTTCTGGCGA+GGG 0.458363 4.4:+4899686 MS.gene069584:CDS
CATTCCCATTCACTATTTCA+CGG 0.460333 4.4:-4896422 None:intergenic
TAATTTAGGTGCCGGGGCTG+TGG 0.461327 4.4:+4897986 MS.gene069584:intron
AATAAATATGCTTGGTATGG+TGG 0.462971 4.4:+4900862 MS.gene069584:CDS
CCTTCGACATGTTTCGATAG+AGG 0.465301 4.4:-4900626 None:intergenic
GCAGGATATCAAATTGTTGA+TGG 0.465544 4.4:+4899857 MS.gene069584:intron
ATTGGATATCAATACTCCCT+TGG 0.466623 4.4:-4899168 None:intergenic
CGATACACAGGACCCATAGA+TGG 0.469476 4.4:-4895386 None:intergenic
AGATCACGTTCCATGTCAAA+TGG 0.469962 4.4:-4901805 None:intergenic
CTTGAACATAAATATTCTCC+AGG 0.470779 4.4:-4901243 None:intergenic
CGAAATAAATATGCTTGGTA+TGG 0.474332 4.4:+4900859 MS.gene069584:CDS
GTTTGCCACTTGAATTTGCC+AGG 0.476179 4.4:-4896527 None:intergenic
TTGGATATCAATACTCCCTT+GGG 0.479790 4.4:-4899167 None:intergenic
ACATGTATCGAGACCTTCAA+TGG 0.481926 4.4:-4895441 None:intergenic
TTAATACAGATTTCTCCTCT+TGG 0.482345 4.4:-4900959 None:intergenic
TTCAACCGAAAGAACCTGTA+TGG 0.482850 4.4:-4901054 None:intergenic
ATTGATGGATGGTTCCATAC+AGG 0.485921 4.4:+4901040 MS.gene069584:CDS
GAGTGTAATAATGTTCTCAT+TGG 0.496079 4.4:-4899186 None:intergenic
CGGGGTGAAAATATATTTGT+GGG 0.499061 4.4:+4898373 MS.gene069584:CDS
ATTTCACGGCGACCCAGCAT+TGG 0.501659 4.4:-4896408 None:intergenic
ATGTACACAAGTGGAACAAC+CGG 0.501819 4.4:+4899140 MS.gene069584:CDS
GGTCGCCGTGAAATAGTGAA+TGG 0.503886 4.4:+4896417 MS.gene069584:CDS
TTCGTTTGGTATTGTTACAA+AGG 0.505019 4.4:-4900840 None:intergenic
AAGCAAGAAGTTGAGAAGAT+CGG 0.505418 4.4:+4898353 MS.gene069584:CDS
TTCTCTCTGTGAAGATTCAA+AGG 0.507257 4.4:+4901576 MS.gene069584:CDS
AAAGATTGCGAAGGAAAAGA+AGG 0.508450 4.4:+4901627 MS.gene069584:CDS
TCGGGGTGAAAATATATTTG+TGG 0.508979 4.4:+4898372 MS.gene069584:CDS
CAATACTCCCTTGGGATCAC+CGG 0.513756 4.4:-4899159 None:intergenic
TGAAGGTCTCGATACATGTT+GGG 0.514347 4.4:+4895445 MS.gene069584:CDS
TTTCAGGCCTGCAATGCTCA+TGG 0.519473 4.4:+4897859 MS.gene069584:intron
TCTGGGATGAAGCAGATACT+AGG 0.520971 4.4:+4899212 MS.gene069584:CDS
CACACTTCCAAATATGGTAA+AGG 0.521446 4.4:-4900890 None:intergenic
GTATCCAACAAATCCAATGC+TGG 0.523005 4.4:+4896395 MS.gene069584:CDS
GGACAAACTTGTATTCAGTA+AGG 0.526680 4.4:+4900445 MS.gene069584:CDS
ATGACTTGGTGATCAAAATT+GGG 0.527547 4.4:+4896564 MS.gene069584:CDS
TTGGAGTCTGTACCTGAAAT+GGG 0.528444 4.4:+4900914 MS.gene069584:CDS
CAAATGTTAATTTAGGTGCC+GGG 0.528739 4.4:+4897979 MS.gene069584:intron
TAATTAAATAATAATGGCTG+AGG 0.529357 4.4:+4895310 None:intergenic
ACATTATTACACTCTTATCT+GGG 0.532870 4.4:+4899195 MS.gene069584:CDS
GGGAAGTCCATTCGTGCTTG+TGG 0.535853 4.4:+4896584 MS.gene069584:CDS
CCCATAACCACAAGCACGAA+TGG 0.537258 4.4:-4896591 None:intergenic
CATTCGTGCTTGTGGTTATG+GGG 0.538296 4.4:+4896592 MS.gene069584:CDS
TTGAAGGTCTCGATACATGT+TGG 0.538408 4.4:+4895444 MS.gene069584:CDS
ATGGTGCCAATTGTCCGGAA+TGG 0.539782 4.4:+4897412 MS.gene069584:CDS
CCAAACGAAATAAATATGCT+TGG 0.541853 4.4:+4900854 MS.gene069584:CDS
ATAAATATGCTTGGTATGGT+GGG 0.541863 4.4:+4900863 MS.gene069584:CDS
AAAGCTAGAGATGCCAAAGA+TGG 0.548876 4.4:+4895359 MS.gene069584:CDS
GACAGAAAAGATTAATAATG+GGG 0.550091 4.4:+4900185 MS.gene069584:CDS
GGTCTTACTGAAACATGTGC+TGG 0.553884 4.4:+4900815 MS.gene069584:intron
CTTTGACAGGATTATTGAAG+AGG 0.554806 4.4:+4899645 MS.gene069584:CDS
ATATGTTGCTGTTGAAAACC+TGG 0.555433 4.4:+4901225 MS.gene069584:CDS
TGGAACAACCGGTGATCCCA+AGG 0.559190 4.4:+4899151 MS.gene069584:CDS
TGATCCAGTTCCATTTGACA+TGG 0.562355 4.4:+4901795 MS.gene069584:CDS
TCCGGAATGGATTATGAGCA+TGG 0.562688 4.4:+4897425 MS.gene069584:CDS
ACTTGTGCTCATATCTTACA+AGG 0.571352 4.4:+4900665 MS.gene069584:CDS
TTTGACAGGATTATTGAAGA+GGG 0.573469 4.4:+4899646 MS.gene069584:CDS
CTGTCAAAGATGTGTGCAAG+TGG 0.575736 4.4:-4899631 None:intergenic
TTGCAGCAAGTTGCATAGCA+TGG 0.578506 4.4:+4900385 MS.gene069584:intron
TCTATCAAGCACACGGGGAA+CGG 0.579381 4.4:-4899916 None:intergenic
TATCCAACAAATCCAATGCT+GGG 0.580274 4.4:+4896396 MS.gene069584:CDS
GTCGCCGTGAAATAGTGAAT+GGG 0.585588 4.4:+4896418 MS.gene069584:CDS
AATCTGTATTAAGGGTGGTA+CGG 0.586331 4.4:+4900970 MS.gene069584:CDS
AAGTTGCATAGCATGGAAAG+AGG 0.589758 4.4:+4900392 MS.gene069584:CDS
TCAAGCAAATGATATGAAGA+TGG 0.591630 4.4:+4901546 MS.gene069584:CDS
TATAACAAACTCCACAGCCC+CGG 0.592002 4.4:-4897997 None:intergenic
AAAGATGGTAGGCCATCTAT+GGG 0.593009 4.4:+4895374 MS.gene069584:CDS
TCGAGACCTTCAATGGGAGG+AGG 0.593915 4.4:-4895434 None:intergenic
GCTATTGAAGACATTTCCAA+AGG 0.599427 4.4:+4898185 MS.gene069584:intron
CATGTATCGAGACCTTCAAT+GGG 0.600238 4.4:-4895440 None:intergenic
AGTTGCATAGCATGGAAAGA+GGG 0.608259 4.4:+4900393 MS.gene069584:CDS
TGTACGATAATGTACACAAG+TGG 0.608897 4.4:+4899131 MS.gene069584:CDS
GCTGCAATAGGTTTCTGGCG+AGG 0.609644 4.4:+4899685 MS.gene069584:CDS
AAGAGGTTATGATTGATGGA+TGG 0.610516 4.4:+4901029 MS.gene069584:CDS
TTATTGAAGAGGGAATCATA+TGG 0.611911 4.4:+4899656 MS.gene069584:CDS
GTATCGTAGCTGTTTCACTA+AGG 0.612811 4.4:+4895403 MS.gene069584:CDS
ACAGAAAAGATTAATAATGG+GGG 0.613428 4.4:+4900186 MS.gene069584:CDS
AAATGTTAATTTAGGTGCCG+GGG 0.619376 4.4:+4897980 MS.gene069584:intron
AATATATTTAAGCTTTCACA+AGG 0.619823 4.4:+4901199 MS.gene069584:CDS
GTTTGTTATATCTCATGCAG+AGG 0.622041 4.4:+4898010 MS.gene069584:CDS
CTAGAGATGCCAAAGATGGT+AGG 0.622804 4.4:+4895363 MS.gene069584:CDS
TGCAATAGGTTTCTGGCGAG+GGG 0.623396 4.4:+4899687 MS.gene069584:CDS
ACGTGAAGACCTCACCAAAG+AGG 0.625025 4.4:+4901012 MS.gene069584:CDS
ACTTCCAAATATGGTAAAGG+AGG 0.625367 4.4:-4900887 None:intergenic
TCAGGTAAAACAAGCTTTAG+GGG 0.625708 4.4:+4900571 MS.gene069584:intron
GGATAAATCATTCATCGTTG+AGG 0.631579 4.4:+4895331 MS.gene069584:CDS
GTGAAACAGCTACGATACAC+AGG 0.631708 4.4:-4895398 None:intergenic
AGAGCTCTTAAAGATTGCGA+AGG 0.638312 4.4:+4901618 MS.gene069584:CDS
CAATCATAACCTCTTTGGTG+AGG 0.639766 4.4:-4901021 None:intergenic
CAAAGATGTGTGCAAGTGGA+AGG 0.647303 4.4:-4899627 None:intergenic
TTACAAAGAAGTGTATGACT+TGG 0.648434 4.4:+4896550 MS.gene069584:CDS
TGTAGCCTGGCAAATTCAAG+TGG 0.648485 4.4:+4896522 MS.gene069584:intron
TGTACACTCTATCAAGCACA+CGG 0.649605 4.4:-4899923 None:intergenic
TTGGGTTAACAACAGCGACA+AGG 0.651672 4.4:-4901498 None:intergenic
CAGGTAAAACAAGCTTTAGG+GGG 0.652277 4.4:+4900572 MS.gene069584:intron
GAAGAGGGAATCATATGGAG+TGG 0.653221 4.4:+4899661 MS.gene069584:CDS
GGAGAAATCTGTATTAAGGG+TGG 0.664225 4.4:+4900965 MS.gene069584:CDS
CCTCTATCGAAACATGTCGA+AGG 0.667299 4.4:+4900626 MS.gene069584:CDS
GGAACAACCGGTGATCCCAA+GGG 0.672635 4.4:+4899152 MS.gene069584:CDS
GCACTTGCAACCAAACCAAG+AGG 0.675688 4.4:+4900944 MS.gene069584:CDS
GCAGTTGCTTAAGTACTACC+AGG 0.676803 4.4:+4901858 MS.gene069584:CDS
AAACAGGAGATATCGGAGAG+TGG 0.676905 4.4:+4901137 MS.gene069584:intron
GTATCGAGACCTTCAATGGG+AGG 0.679829 4.4:-4895437 None:intergenic
TGAAGAAAAGAAGATACCTG+AGG 0.695780 4.4:+4898049 MS.gene069584:CDS
CATTGACGAGATGTACAAGA+CGG 0.697605 4.4:+4902180 MS.gene069584:CDS
TACACTCTATCAAGCACACG+GGG 0.700121 4.4:-4899921 None:intergenic
GTGCTTGATAGAGTGTACAC+AGG 0.706474 4.4:+4899927 MS.gene069584:CDS
GAGCACAAGTCACAACACGC+AGG 0.710027 4.4:-4900652 None:intergenic
TGGTAGTACTTAAGCAACTG+CGG 0.711513 4.4:-4901856 None:intergenic
GTACACTCTATCAAGCACAC+GGG 0.715609 4.4:-4899922 None:intergenic
GCAAGAAGTTGAGAAGATCG+GGG 0.748481 4.4:+4898355 MS.gene069584:CDS
GGAATGGATTATGAGCATGG+AGG 0.791685 4.4:+4897428 MS.gene069584:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content


Chromosome Type Strat End Strand Name
chr4.4 gene 4895323 4902210 4895323 ID=MS.gene069584
chr4.4 mRNA 4895323 4902210 4895323 ID=MS.gene069584.t1;Parent=MS.gene069584
chr4.4 exon 4895323 4895477 4895323 ID=MS.gene069584.t1.exon1;Parent=MS.gene069584.t1
chr4.4 CDS 4895323 4895477 4895323 ID=cds.MS.gene069584.t1;Parent=MS.gene069584.t1
chr4.4 exon 4896380 4896443 4896380 ID=MS.gene069584.t1.exon2;Parent=MS.gene069584.t1
chr4.4 CDS 4896380 4896443 4896380 ID=cds.MS.gene069584.t1;Parent=MS.gene069584.t1
chr4.4 exon 4896527 4896616 4896527 ID=MS.gene069584.t1.exon3;Parent=MS.gene069584.t1
chr4.4 CDS 4896527 4896616 4896527 ID=cds.MS.gene069584.t1;Parent=MS.gene069584.t1
chr4.4 exon 4897393 4897449 4897393 ID=MS.gene069584.t1.exon4;Parent=MS.gene069584.t1
chr4.4 CDS 4897393 4897449 4897393 ID=cds.MS.gene069584.t1;Parent=MS.gene069584.t1
chr4.4 exon 4897865 4897913 4897865 ID=MS.gene069584.t1.exon5;Parent=MS.gene069584.t1
chr4.4 CDS 4897865 4897913 4897865 ID=cds.MS.gene069584.t1;Parent=MS.gene069584.t1
chr4.4 exon 4897994 4898070 4897994 ID=MS.gene069584.t1.exon6;Parent=MS.gene069584.t1
chr4.4 CDS 4897994 4898070 4897994 ID=cds.MS.gene069584.t1;Parent=MS.gene069584.t1
chr4.4 exon 4898186 4898225 4898186 ID=MS.gene069584.t1.exon7;Parent=MS.gene069584.t1
chr4.4 CDS 4898186 4898225 4898186 ID=cds.MS.gene069584.t1;Parent=MS.gene069584.t1
chr4.4 exon 4898318 4898412 4898318 ID=MS.gene069584.t1.exon8;Parent=MS.gene069584.t1
chr4.4 CDS 4898318 4898412 4898318 ID=cds.MS.gene069584.t1;Parent=MS.gene069584.t1
chr4.4 exon 4899083 4899250 4899083 ID=MS.gene069584.t1.exon9;Parent=MS.gene069584.t1
chr4.4 CDS 4899083 4899250 4899083 ID=cds.MS.gene069584.t1;Parent=MS.gene069584.t1
chr4.4 exon 4899598 4899708 4899598 ID=MS.gene069584.t1.exon10;Parent=MS.gene069584.t1
chr4.4 CDS 4899598 4899708 4899598 ID=cds.MS.gene069584.t1;Parent=MS.gene069584.t1
chr4.4 exon 4899861 4899948 4899861 ID=MS.gene069584.t1.exon11;Parent=MS.gene069584.t1
chr4.4 CDS 4899861 4899948 4899861 ID=cds.MS.gene069584.t1;Parent=MS.gene069584.t1
chr4.4 exon 4900181 4900226 4900181 ID=MS.gene069584.t1.exon12;Parent=MS.gene069584.t1
chr4.4 CDS 4900181 4900226 4900181 ID=cds.MS.gene069584.t1;Parent=MS.gene069584.t1
chr4.4 exon 4900391 4900466 4900391 ID=MS.gene069584.t1.exon13;Parent=MS.gene069584.t1
chr4.4 CDS 4900391 4900466 4900391 ID=cds.MS.gene069584.t1;Parent=MS.gene069584.t1
chr4.4 exon 4900575 4900692 4900575 ID=MS.gene069584.t1.exon14;Parent=MS.gene069584.t1
chr4.4 CDS 4900575 4900692 4900575 ID=cds.MS.gene069584.t1;Parent=MS.gene069584.t1
chr4.4 exon 4900816 4901061 4900816 ID=MS.gene069584.t1.exon15;Parent=MS.gene069584.t1
chr4.4 CDS 4900816 4901061 4900816 ID=cds.MS.gene069584.t1;Parent=MS.gene069584.t1
chr4.4 exon 4901143 4901282 4901143 ID=MS.gene069584.t1.exon16;Parent=MS.gene069584.t1
chr4.4 CDS 4901143 4901282 4901143 ID=cds.MS.gene069584.t1;Parent=MS.gene069584.t1
chr4.4 exon 4901466 4901648 4901466 ID=MS.gene069584.t1.exon17;Parent=MS.gene069584.t1
chr4.4 CDS 4901466 4901648 4901466 ID=cds.MS.gene069584.t1;Parent=MS.gene069584.t1
chr4.4 exon 4901760 4901879 4901760 ID=MS.gene069584.t1.exon18;Parent=MS.gene069584.t1
chr4.4 CDS 4901760 4901879 4901760 ID=cds.MS.gene069584.t1;Parent=MS.gene069584.t1
chr4.4 exon 4902175 4902210 4902175 ID=MS.gene069584.t1.exon19;Parent=MS.gene069584.t1
chr4.4 CDS 4902175 4902210 4902175 ID=cds.MS.gene069584.t1;Parent=MS.gene069584.t1
Gene Sequence

>MS.gene069584

ATGGCTGAGGATAAATCATTCATCGTTGAGGTTGAGAAAGCTAGAGATGCCAAAGATGGTAGGCCATCTATGGGTCCTGTGTATCGTAGCTGTTTCACTAAGGATATCTCTCCTCCTCCCATTGAAGGTCTCGATACATGTTGGGATGTTTTCAGGTCACTTTCATTTCATTCTCATAGATCACAATATTATTTTCATTTCTACTTGTCACAACATCTAAAATGTCTATGTCTATCATTTCTTCACGTGTTAATTTGGCACGATTTGATTGAATGAATTTGCATAGTTTTAAATTGTGGTTCGCATAAGTAATTGCAGTGGGTAATGTGAGTGAAGGTTTTTGTTTTGTGTAAGCATTGTCAAAGTAGAACAATGAGATTGAACTTGTTGAGAGATCTCTAATTGGATCTCCTTTGAAAAATAAATCTCTTGAAAACAAGTCATACTTTCTAGCTCTATAAATTTCGAATTAAGATTACTTTGATAGGAAGATATATTGTAGATTTCATCAAAAAGTTATAGAAAACATGTAACTAGACACAATTAAAAATACATATAAAATCCATGTAAAACATAAATTTAAAATGAGAACTAATATTTTAAAAGTTGAGTGAGTGTAAAGATAATATATAATATTGTCATTTATGTTTTAGAATAGGAATAAATAATCTAAACAATGGTAAAATACAACTAAAAGTGTGTCTAATGTGTGAAAGCAATGCTTCACAAGGTAACAATGGTTAGAGTTGATATACTTTTTGGAAAAAAAACAGAATGAGAAAAAATGTACATACAACAAGAGGGACACATGAAGTGGGTTATGGTTGGCTTTTTTACTATATTTCATCTATGCAACACCGATATTTCGATCTGATCGAAAGTGTGTCCTTGCCGACACGGCACGACACGATATCGATACATGCAGATACATTCAATTTTTTTCTCTTCAAATTATTACATGTAAATGTCGTGTCCGTGTATGTTTTAGTGATTCACAGTATTTCGTAAGAGATTTAACAAATTAATTTAGTTCTGATTGTTTTTGAGGGTTATACAGGACTGCTGTTGAGAAGTATCCAACAAATCCAATGCTGGGTCGCCGTGAAATAGTGAATGGGAATGTGAACACTCTTTTTCCCTCTCTTTCATTTTTGCCATTATGCAAATTTATTGTTACTTTTTGAATTGTGTCACACTCTTGTAGCCTGGCAAATTCAAGTGGCAAACTTACAAAGAAGTGTATGACTTGGTGATCAAAATTGGGAAGTCCATTCGTGCTTGTGGTTATGGGGAAGTAAGTCACATGTGCTTATTCTTGAGGATTGCTTTCACCTTTTCTGATTATTTTTGCATAGGCGATGTTTGGATAAACAGTTTAATAAAGTGTTTATAGCATCATAGCTTACCATATAAGTGCTTATGTATTAGCAATTTCGAAAACAAAAAAATACTATAAAGTCAAACTGCTATCATGAGTTATAAGCTGTTTTCATGTGCCATCCTTGAGAACTAATAGAAATAATCGGGAATCAGCTTATAGACATGTCATTGTTATCAATGTAGGTTCAGAGAGAATAACAAAACAAAGGCGGCTGCTAGAGTTTCATAAAATATCAAATGGCATAGCATATAAAAATAGAGTTACGAGAGATTATATGGCAAAACCTAAACGGCCAGACTAAGCTAGAAATATAAAGACCCGATAACTTAAGTCAAATATTTTATTATCTAACAATCATAAGTTGTTATAAACTCTCACAAGTGTTTATGTCACTAGATCAGTTGAAATAAATCAATCCAAACAAGGCTCTTTTATCTTCTTTGATTTGTCACTGCAATTCCTGGATTCTCACTTCTTCTGTTATCCGTGACATAATGTTTTGCATACCAAAAACTAATTCAAAATTCTCTTTATTTCTTTCTCTTTTACAATTATATATTCTTGCTCTTACACTTTGAGATCTTCATGAATAGCTTGGTCATGAAGGACTATGGGATCTTTTGCCTCATATTAGTATCTTTTCTTGTATTGTGTGTATGCAAGCTAAATATATATATTGCTTGTTTTAGGGAGTAAAATGCGGCATTTATGGTGCCAATTGTCCGGAATGGATTATGAGCATGGAGGTACATTTTATTTTGAACTTATTTATTTATTCATGTTATCTTCTATGCATATGATTTTTCTTTTTACTCTGTTCTTTGAAGGTTTCAGTTGTAGTTATACGTTGATATTTGCTTGTACCTTTGTGTTGTATATTTATCAGTTTTTTAATATATTGTTAATGCAATAATGTGATTGTTCTAGTCTGTTTATGAATTATGATCCATTTTGAACATGGAAGAAAAACAAGGCTTTTTTTAAATTGAAACTTTGTTTCATCTATTGAATATTTGTTCTTATACAATGTTTGGTTCCATTATTTGGTTCTCTCTTGGCCATCCTTTGTCACCAATAGCGATGATCAATTTTAGTGTTGCTAAGCTAGTTTCTGTTATCCTTTTAACTTAACATGTCTTACATATTTTAAGATAATTTCAGGCCTGCAATGCTCATGGACTTTATTGTGTTCCTTTATATGATACTTTAGGTATGATCCCACCTTCTTTGGCATTTATCTACCTATATTGTGTTACCACTTTAATATTTATTTTCCAAATGTTAATTTAGGTGCCGGGGCTGTGGAGTTTGTTATATCTCATGCAGAGGTCTCAATAGCATTTGTTGAAGAAAAGAAGATACCTGAGGTTCATCTATTGAATTAATGATCCGTGTGGTAGCAATGTATTATTTGGAAGGCTTAGAAAGTGTGATCTGCAATTTTCTATAACTAAGTCATATATTTTTTCTGAAACTTTTCAGCTATTGAAGACATTTCCAAAGGCAGCAGAGTATCTCAAGAGTAAGTTTCATTGATTTTCTCCATTAATCCCTTTCCTAAAATGAAAGAAGCAACTACCTTTATTTGTTTAATTATTTATCTATTTTGTGTAGCAATTGTGAGCTTTGGAAAAGTTTCTGCAGAACAAAAGCAAGAAGTTGAGAAGATCGGGGTGAAAATATATTTGTGGGATGAATTTTTAGAAGTGGTGGGTTATTATTACTCTTTTTCCTCCTATCTGTTGCAAATTTGTCATGAAAAGGCTCCTTCTAGATCATAGTCACATAATTCGCGGTTCGTTCCGGTACGCGGTACGGGTATGGGTACGGGTTCGCGGTACGCGACAGGAGAAGAATTCGATACGTATAGGGGTATACAGCTTCGGTACGCTCCGGTTCGTAGTAGTAATAATACCTAAATAAATATAGTGAGCTATAAAAAAAAAGTATTTTTCTTTACCTAATTTCTTCTACCAAGTTTCTCACTCTCCACTCTTTGTTCATGTTCTTCTCACTCAGTCCTCAACTCATGGTGATTCATTTTATATTTACTTTGTAGTCTAAACCTTTTTTTCAACCAATCAGCGGTAACCATGCTTTGGTACGCTTGGTTCGCTGGTTTGTGCGTACCAATACGCCGAAGTACCCGAATTCGGTACTCACGTACCAATTCGCAATTCTCCCTTGATTACAAGAATTATGTGACTATGTTCTAGATTTACTTGGCTGTATCACAAAATATTAGATACACTTCTTGAATTGCAAGTTTTGTTTCTAACACTCAGTCCAGAAATGACAAGAGGAAGTTACGTGTTACAATTGTTTTATCGATATTTTGTGGTTGGATTGCTGATTCGAGTTTTTTAATGTATGTCACAGGGTCATGATAAGAATTTTGATCTTCCTGAAAAAAAGAAAAGCGACATATGTACGATAATGTACACAAGTGGAACAACCGGTGATCCCAAGGGAGTATTGATATCCAATGAGAACATTATTACACTCTTATCTGGGATGAAGCAGATACTAGGTAGTTGCAATGAACAAGTAAGTAGTTTTCTATTAACACATATTTTTCTTGCAATTATATGAACACACAATGGTCAAGGGACTCCTAAAATGCCTACATCTTCCCATTTAAGTAAGCATTTTGGTCTTCTAATATGAATCTTAAGCAGTTTGATCAAATACTGCTGCACAACAATGATATAGCGCCGCTACATCTGCTGTTTGACAATATTTTGTACTAAATAGCGTATCGTGGAACAATAACAATTTATTCAAATTCCGCAACATCATTGTGTGCTGCTATTCAACAAAGCTAAATTGCTCCTAAAATGCTATTTCATTTTTTGGATAATGAAAGTTCCGCTATCGGTTGTCTCTGCTTGCAGTTGCATCAGAAAGATGTATACATATCATACCTTCCACTTGCACACATCTTTGACAGGATTATTGAAGAGGGAATCATATGGAGTGGTGCTGCAATAGGTTTCTGGCGAGGGGTGAGTATAGATATATACGTGATAAATCTTTCATTACTGAATGAAGGCTTGATGTGATTATAGTATATTGGATTTTCTTAAATTTAATATCAAGTAAGTCAAAATTTGTATTTGATATTAATATGAACACCATTTATTCTATGTGGCTGCAGGATATCAAATTGTTGATGGAAGACATTGCTGAGTTAAAACCAACTATTTTTTGTGCCGTTCCCCGTGTGCTTGATAGAGTGTACACAGGTAATTTTTTGTCGTTGCTTACAGTTTAGTCATCTTTCCTACACCATGTGATGTTTGTGAAAAAATTATAATTTCATAAAGAACAAAACTATACTCTTAAGATAGTTCTTATTAGTCACTGAAGTGGTCTTTTTATTGAATTCCTTATTCTAATTTCCATCTCTTTGAACAATTTTTCCTCTCACCAATAATAATAATGCAATGAAAATCTCAACCTCCATCTGTTATACAGGTTTGACAGAAAAGATTAATAATGGGGGCTTTGTGAAACAGACGATGTTCAATTGGGCCTACACATAGGAAGTAACGTGTTTTAAATGATGGAAATATGACTAATTATTGTATGAGTGTTCCTATGTAAATCAAAGCACACAAAATCTCATTTAGAAGATGATAATGTATTTCAATGTTCTGATAATATAAACTATGAAATATGTTGCAGCAAGTTGCATAGCATGGAAAGAGGGCAAAAACATGAAACGGCAGCTCCATTAATGGACAAACTTGTATTCAGTAAGGTGATTGTAGTTTCACATATTTTAATTCACTACCGTCTTGATTCGAACAAACAATAACCGTAACAAAATTGCTCTGTACTAATAATAATGTTTTTGCAACATGATCAGGTAAAACAAGCTTTAGGGGGTAATGTACGCATTGTTTTGTCTGGAGCTGCGCCTCTATCGAAACATGTCGAAGGTTTCCTGCGTGTTGTGACTTGTGCTCATATCTTACAAGGATATGGTATGTTCTTTCTCAACATTTGGAGTATCATGAACTTCTGTTACATTTATGTCTGTGATTATTGATGTATTAAATCAGCAAAAGATTCTTTTTAAACATGGACATGATTTTTGGCCTTTGTAGGTCTTACTGAAACATGTGCTGGATCCTTTGTAACAATACCAAACGAAATAAATATGCTTGGTATGGTGGGACCTCCTTTACCATATTTGGAAGTGTGTTTGGAGTCTGTACCTGAAATGGGATATGATGCACTTGCAACCAAACCAAGAGGAGAAATCTGTATTAAGGGTGGTACGGTTTTCGCAGGGTATCACAAACGTGAAGACCTCACCAAAGAGGTTATGATTGATGGATGGTTCCATACAGGTTCTTTCGGTTGAACTTTAGATAATCTATACTTGTTGTTGTACATGGTCACATTTTATTCATGCATGATCTATTAAACAGGAGATATCGGAGAGTGGCAACCTAATGGAAGCATGAAAATTATCGATCGAAAGAAGAATATATTTAAGCTTTCACAAGGAGAATATGTTGCTGTTGAAAACCTGGAGAATATTTATGTTCAAGTTAATGCTATTGAATCCGTATGTATTCTAAACCCTTTGAATAGTTTAGAAGTACTATTTTGCAATGAATTTGTTTTCCTTTGAAGGAGTTAAGAAAATATGTGGAGTAATGATATTAAAAGGTTTAGTTTCAGCTTCTTTTCATTAGTAGACAATGTTTTGTGACATGTTTATATTGTGTTTTATCTTTCTTTGTTTCAGATATGGATCTATGGAAACAGTTTTGAGCACTTCCTTGTCGCTGTTGTTAACCCAAGCAAGAAAGCACTTGAAGATTGGGCTCAAGCAAATGATATGAAGATGGACTTTAATTCTCTCTGTGAAGATTCAAAGGCGAAACATTACATACTTGGAGAGCTCTTAAAGATTGCGAAGGAAAAGAAGGTTCTATTTAGCTAATGTCTCATAATATTTCTCTAATTTGTATAATTTGATGAATTTGAGAACACCTCACTTGAAGCTTTATGCTGATCATGATTTTTGTTCTTTTTGTAGTTGAAAGGTTTTGAGTTTGTTAAAGTTGTTCATCTTGATCCAGTTCCATTTGACATGGAACGTGATCTTCTCACACCAACATTTAAGAAAAAGAGACCGCAGTTGCTTAAGTACTACCAGGTATAACTTGTTAAAATTTTGGATTATGTTATGTTTCACTAAGTTGACTAATCACTCTGATAATTGGACATTTGAACTATAACTGAATGTCAGAATTGACACCTTAAGTGCAAATTCAAGGACATAAGTACAGTTTATAAAGTTTGCCCCATAAAATATCTTTCTAATTCCATTGAACTTCTCAATGTGTCAAACCAATTTTCTTTTGCTCCTTTTATTTATTTATTTATCTATTTATTGTACAAACTTAAGCACATACTTGTTTCAGTACTGATTGTAATATTTTTGTGCACAGAATATCATTGACGAGATGTACAAGACGGCCAAATAA
Protein sequence

>MS.gene069584.t1

MAEDKSFIVEVEKARDAKDGRPSMGPVYRSCFTKDISPPPIEGLDTCWDVFRTAVEKYPTNPMLGRREIVNGNPGKFKWQTYKEVYDLVIKIGKSIRACGYGEGVKCGIYGANCPEWIMSMEACNAHGLYCVPLYDTLGAGAVEFVISHAEVSIAFVEEKKIPELLKTFPKAAEYLKTIVSFGKVSAEQKQEVEKIGVKIYLWDEFLEVGHDKNFDLPEKKKSDICTIMYTSGTTGDPKGVLISNENIITLLSGMKQILGSCNEQLHQKDVYISYLPLAHIFDRIIEEGIIWSGAAIGFWRGDIKLLMEDIAELKPTIFCAVPRVLDRVYTGLTEKINNGGFVKQTIKLHSMERGQKHETAAPLMDKLVFSKVKQALGGNVRIVLSGAAPLSKHVEGFLRVVTCAHILQGYGLTETCAGSFVTIPNEINMLGMVGPPLPYLEVCLESVPEMGYDALATKPRGEICIKGGTVFAGYHKREDLTKEVMIDGWFHTGDIGEWQPNGSMKIIDRKKNIFKLSQGEYVAVENLENIYVQVNAIESIWIYGNSFEHFLVAVVNPSKKALEDWAQANDMKMDFNSLCEDSKAKHYILGELLKIAKEKKLKGFEFVKVVHLDPVPFDMERDLLTPTFKKKRPQLLKYYQNIIDEMYKTAK