Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene069628.t1 | XP_024634712.1 | 90.3 | 155 | 15 | 0 | 1 | 155 | 1 | 155 | 8.10E-78 | 299.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene069628.t1 | A0A396IUK6 | 78.3 | 157 | 32 | 1 | 1 | 155 | 1 | 157 | 1.5e-65 | 258.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene053356 | MS.gene069628 | 0.827911 | 1.20E-54 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene069628.t1 | MTR_6g066340 | 56.688 | 157 | 24 | 2 | 1 | 155 | 37 | 151 | 3.52e-43 | 141 |
| MS.gene069628.t1 | MTR_2g099105 | 62.500 | 88 | 26 | 2 | 1 | 81 | 1 | 88 | 6.78e-28 | 99.8 |
| MS.gene069628.t1 | MTR_7g099240 | 36.842 | 114 | 49 | 4 | 40 | 153 | 83 | 173 | 1.03e-12 | 63.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene069628.t1 | AT1G19260 | 38.583 | 127 | 71 | 4 | 24 | 149 | 11 | 131 | 7.57e-25 | 99.8 |
| MS.gene069628.t1 | AT4G09660 | 37.795 | 127 | 72 | 4 | 24 | 149 | 11 | 131 | 5.62e-23 | 94.4 |
Find 33 sgRNAs with CRISPR-Local
Find 36 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CGAGGAAAACTTGACAAATT+TGG | 0.204759 | 3.4:+27354670 | None:intergenic |
| CCAATCTTTAAATCCGTCTT+TGG | 0.280934 | 3.4:+27354489 | None:intergenic |
| CTCACTTGGTCTTGAATATC+AGG | 0.303118 | 3.4:+27354724 | None:intergenic |
| CCAAAGACGGATTTAAAGAT+TGG | 0.323002 | 3.4:-27354489 | MS.gene069628:CDS |
| CTTGACAAATTTGGTTGCAT+TGG | 0.334233 | 3.4:+27354679 | None:intergenic |
| CTCATTTGGATTAAATTCAT+TGG | 0.341888 | 3.4:+27354792 | None:intergenic |
| GGATCACACACAATCTCATT+TGG | 0.382227 | 3.4:+27354778 | None:intergenic |
| AAAATCTTGCAAGATCATAT+TGG | 0.412713 | 3.4:-27354454 | MS.gene069628:CDS |
| GGTTCTTCTACTTCGACTTC+CGG | 0.422132 | 3.4:+27354829 | None:intergenic |
| TTAAATTCATTGGCCACATT+AGG | 0.465850 | 3.4:+27354802 | None:intergenic |
| CCCGCGAGGGCTGAGTACTT+TGG | 0.465860 | 3.4:-27354526 | MS.gene069628:CDS |
| GCTCTTGTTTGACTCCCAAA+TGG | 0.475954 | 3.4:+27354643 | None:intergenic |
| TTCGATGTCTTCACCAAAGA+CGG | 0.484619 | 3.4:-27354502 | MS.gene069628:CDS |
| TAATGTGAATGTTGTGCAAC+CGG | 0.488213 | 3.4:-27354848 | MS.gene069628:CDS |
| GAGGAGGGCATATATCTTGA+AGG | 0.496344 | 3.4:-27354704 | MS.gene069628:CDS |
| GATTGAATACAGTGAGTTCA+AGG | 0.498483 | 3.4:-27354587 | MS.gene069628:CDS |
| AATGAGATTGTGTGTGATCC+AGG | 0.507491 | 3.4:-27354775 | MS.gene069628:CDS |
| AGATATATGCCCTCCTCACT+TGG | 0.509334 | 3.4:+27354710 | None:intergenic |
| TGAATTTGTTTCCTACGACC+TGG | 0.542287 | 3.4:+27354757 | None:intergenic |
| AATTCATTGGCCACATTAGG+CGG | 0.563218 | 3.4:+27354805 | None:intergenic |
| CTGATATTCAAGACCAAGTG+AGG | 0.582545 | 3.4:-27354723 | MS.gene069628:CDS |
| CCAAAGTACTCAGCCCTCGC+GGG | 0.584490 | 3.4:+27354526 | None:intergenic |
| AGAAGAACCACCGCCTAATG+TGG | 0.590890 | 3.4:-27354815 | MS.gene069628:CDS |
| CATGGTATGAGAAGTATGCA+TGG | 0.600857 | 3.4:-27354609 | MS.gene069628:CDS |
| ACCAAAGTACTCAGCCCTCG+CGG | 0.604508 | 3.4:+27354525 | None:intergenic |
| TCATTGGCCACATTAGGCGG+TGG | 0.616172 | 3.4:+27354808 | None:intergenic |
| TATTCAAGACCAAGTGAGGA+GGG | 0.618445 | 3.4:-27354719 | MS.gene069628:CDS |
| ATATTCAAGACCAAGTGAGG+AGG | 0.627378 | 3.4:-27354720 | MS.gene069628:CDS |
| TTGTGTGTGATCCAGGTCGT+AGG | 0.629379 | 3.4:-27354768 | MS.gene069628:CDS |
| AGGAGGGCATATATCTTGAA+GGG | 0.635940 | 3.4:-27354703 | MS.gene069628:CDS |
| TGACTCCCAAATGGAGTACG+AGG | 0.701429 | 3.4:+27354652 | None:intergenic |
| TTCTCTTTAAGAAACCCGCG+AGG | 0.710825 | 3.4:-27354540 | MS.gene069628:CDS |
| TCTCTTTAAGAAACCCGCGA+GGG | 0.713663 | 3.4:-27354539 | MS.gene069628:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AAAATCTTGCAAGATCATAT+TGG | - | chr3.4:27354849-27354868 | MS.gene069628:CDS | 25.0% |
| ! | CTCATTTGGATTAAATTCAT+TGG | + | chr3.4:27354514-27354533 | None:intergenic | 25.0% |
| TTAAATTCATTGGCCACATT+AGG | + | chr3.4:27354504-27354523 | None:intergenic | 30.0% | |
| CCAATCTTTAAATCCGTCTT+TGG | + | chr3.4:27354817-27354836 | None:intergenic | 35.0% | |
| CGAGGAAAACTTGACAAATT+TGG | + | chr3.4:27354636-27354655 | None:intergenic | 35.0% | |
| CTTGACAAATTTGGTTGCAT+TGG | + | chr3.4:27354627-27354646 | None:intergenic | 35.0% | |
| GATTGAATACAGTGAGTTCA+AGG | - | chr3.4:27354716-27354735 | MS.gene069628:CDS | 35.0% | |
| TAATGTGAATGTTGTGCAAC+CGG | - | chr3.4:27354455-27354474 | MS.gene069628:CDS | 35.0% | |
| ! | CCAAAGACGGATTTAAAGAT+TGG | - | chr3.4:27354814-27354833 | MS.gene069628:CDS | 35.0% |
| !!! | CAAGAGCATTTTGTAGATCA+TGG | - | chr3.4:27354676-27354695 | MS.gene069628:CDS | 35.0% |
| AATGAGATTGTGTGTGATCC+AGG | - | chr3.4:27354528-27354547 | MS.gene069628:CDS | 40.0% | |
| AATTCATTGGCCACATTAGG+CGG | + | chr3.4:27354501-27354520 | None:intergenic | 40.0% | |
| ATATTCAAGACCAAGTGAGG+AGG | - | chr3.4:27354583-27354602 | MS.gene069628:CDS | 40.0% | |
| CTGATATTCAAGACCAAGTG+AGG | - | chr3.4:27354580-27354599 | MS.gene069628:CDS | 40.0% | |
| GGATCACACACAATCTCATT+TGG | + | chr3.4:27354528-27354547 | None:intergenic | 40.0% | |
| TATTCAAGACCAAGTGAGGA+GGG | - | chr3.4:27354584-27354603 | MS.gene069628:CDS | 40.0% | |
| TTCGATGTCTTCACCAAAGA+CGG | - | chr3.4:27354801-27354820 | MS.gene069628:CDS | 40.0% | |
| ! | AGGAGGGCATATATCTTGAA+GGG | - | chr3.4:27354600-27354619 | MS.gene069628:CDS | 40.0% |
| ! | AGTTTTCCTCGTACTCCATT+TGG | - | chr3.4:27354645-27354664 | MS.gene069628:CDS | 40.0% |
| ! | CATGGTATGAGAAGTATGCA+TGG | - | chr3.4:27354694-27354713 | MS.gene069628:CDS | 40.0% |
| ! | GTTTTCCTCGTACTCCATTT+GGG | - | chr3.4:27354646-27354665 | MS.gene069628:CDS | 40.0% |
| ! | TGAATTTGTTTCCTACGACC+TGG | + | chr3.4:27354549-27354568 | None:intergenic | 40.0% |
| !! | CTCACTTGGTCTTGAATATC+AGG | + | chr3.4:27354582-27354601 | None:intergenic | 40.0% |
| AGATATATGCCCTCCTCACT+TGG | + | chr3.4:27354596-27354615 | None:intergenic | 45.0% | |
| GGTTCTTCTACTTCGACTTC+CGG | + | chr3.4:27354477-27354496 | None:intergenic | 45.0% | |
| ! | GAGGAGGGCATATATCTTGA+AGG | - | chr3.4:27354599-27354618 | MS.gene069628:CDS | 45.0% |
| ! | GCTCTTGTTTGACTCCCAAA+TGG | + | chr3.4:27354663-27354682 | None:intergenic | 45.0% |
| ! | TCTCTTTAAGAAACCCGCGA+GGG | - | chr3.4:27354764-27354783 | MS.gene069628:CDS | 45.0% |
| ! | TTCTCTTTAAGAAACCCGCG+AGG | - | chr3.4:27354763-27354782 | MS.gene069628:CDS | 45.0% |
| AGAAGAACCACCGCCTAATG+TGG | - | chr3.4:27354488-27354507 | MS.gene069628:CDS | 50.0% | |
| TGACTCCCAAATGGAGTACG+AGG | + | chr3.4:27354654-27354673 | None:intergenic | 50.0% | |
| TTGTGTGTGATCCAGGTCGT+AGG | - | chr3.4:27354535-27354554 | MS.gene069628:CDS | 50.0% | |
| TCATTGGCCACATTAGGCGG+TGG | + | chr3.4:27354498-27354517 | None:intergenic | 55.0% | |
| ! | ACCAAAGTACTCAGCCCTCG+CGG | + | chr3.4:27354781-27354800 | None:intergenic | 55.0% |
| ! | CCAAAGTACTCAGCCCTCGC+GGG | + | chr3.4:27354780-27354799 | None:intergenic | 60.0% |
| CCCGCGAGGGCTGAGTACTT+TGG | - | chr3.4:27354777-27354796 | MS.gene069628:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.4 | gene | 27354426 | 27354899 | 27354426 | ID=MS.gene069628 |
| chr3.4 | mRNA | 27354426 | 27354899 | 27354426 | ID=MS.gene069628.t1;Parent=MS.gene069628 |
| chr3.4 | exon | 27354426 | 27354899 | 27354426 | ID=MS.gene069628.t1.exon1;Parent=MS.gene069628.t1 |
| chr3.4 | CDS | 27354426 | 27354899 | 27354426 | ID=cds.MS.gene069628.t1;Parent=MS.gene069628.t1 |
| Gene Sequence |
| Protein sequence |
