Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050799 | MS.gene06980 | 0.803545 | 3.31E-49 | -1.69E-46 |
| MS.gene053396 | MS.gene06980 | 0.808525 | 2.97E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 33 sgRNAs with CRISPR-Local
Find 102 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATAATTGGTTACATGCTTTA+AGG | 0.175982 | 7.3:-6353217 | MS.gene06980:CDS |
| TATGGAGATTTGTTGATAAT+TGG | 0.228668 | 7.3:-6353232 | MS.gene06980:CDS |
| ATCACCGTTGCCTTCTGTTT+TGG | 0.265946 | 7.3:-6354289 | MS.gene06980:CDS |
| GGAAACTTATTAACAACATT+TGG | 0.312904 | 7.3:-6352570 | MS.gene06980:CDS |
| TTAAGGCCGCGATCCTATAT+TGG | 0.324613 | 7.3:-6353200 | MS.gene06980:intron |
| TATGCATAACTAAGAATTGC+TGG | 0.351510 | 7.3:+6353916 | None:intergenic |
| AGAAGGCAACGGTGATTCTT+GGG | 0.359653 | 7.3:+6354296 | None:intergenic |
| GCTCAAATGTTATCATCATT+TGG | 0.360572 | 7.3:+6354343 | None:intergenic |
| TTGATATAACTTACCAATAT+AGG | 0.371517 | 7.3:+6353187 | None:intergenic |
| CAGAAGGCAACGGTGATTCT+TGG | 0.374225 | 7.3:+6354295 | None:intergenic |
| TAATTGAAATGCAGGTCATA+TGG | 0.387079 | 7.3:-6352591 | MS.gene06980:intron |
| TCTGTGAAAAGTTCTACTTA+TGG | 0.418231 | 7.3:-6353951 | MS.gene06980:intron |
| AGCTAATAAAGAACACTTAC+CGG | 0.438040 | 7.3:-6353744 | MS.gene06980:intron |
| CTTAGTTATGCATAATAGAA+AGG | 0.445577 | 7.3:-6353907 | MS.gene06980:intron |
| AAGGAATTTCAAGGAAATTG+AGG | 0.455849 | 7.3:-6352720 | MS.gene06980:intron |
| TACTGTTGGAAGGAATTTCA+AGG | 0.470034 | 7.3:-6352729 | MS.gene06980:CDS |
| TCCAGAAAACACTATATGTT+AGG | 0.479477 | 7.3:-6352790 | MS.gene06980:CDS |
| ACATATCCCAACAGCAGCTA+TGG | 0.487065 | 7.3:-6353250 | MS.gene06980:intron |
| AACTTATTAACAACATTTGG+AGG | 0.499331 | 7.3:-6352567 | MS.gene06980:CDS |
| AACTATGCTGCTGATACTGT+TGG | 0.502229 | 7.3:-6352743 | MS.gene06980:CDS |
| GCAACGGTGATTCTTGGGGC+TGG | 0.507654 | 7.3:+6354301 | None:intergenic |
| CTTGGTCAAGTTCATGAGAA+TGG | 0.523957 | 7.3:+6354247 | None:intergenic |
| TCATGAGAATGGCATGGAGA+AGG | 0.552357 | 7.3:+6354258 | None:intergenic |
| GATGATAACATTTGAGCAGC+TGG | 0.553928 | 7.3:-6354337 | MS.gene06980:CDS |
| CATCATTTGGATCCAGCCAA+TGG | 0.555879 | 7.3:+6354356 | None:intergenic |
| GGAATTTGATCCAAAACAGA+AGG | 0.564596 | 7.3:+6354279 | None:intergenic |
| GCTTTGCAATGAAGAAGAAG+AGG | 0.591800 | 7.3:-6353795 | MS.gene06980:CDS |
| GCAGCATAGTTCTTACGCAG+TGG | 0.627252 | 7.3:+6352755 | None:intergenic |
| GAAGGCAACGGTGATTCTTG+GGG | 0.628139 | 7.3:+6354297 | None:intergenic |
| TCAAGTTCATGAGAATGGCA+TGG | 0.637623 | 7.3:+6354252 | None:intergenic |
| ATGCTGCTGATACTGTTGGA+AGG | 0.648055 | 7.3:-6352739 | MS.gene06980:CDS |
| TGATCCAAAACAGAAGGCAA+CGG | 0.651613 | 7.3:+6354285 | None:intergenic |
| AACTTACCAATATAGGATCG+CGG | 0.659346 | 7.3:+6353194 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATGGTAATATAATAAATAAA+CGG | - | chr7.3:6352908-6352927 | MS.gene06980:intron | 10.0% |
| !! | ATATTTAGCATCTTTAAATA+AGG | + | chr7.3:6354229-6354248 | None:intergenic | 15.0% |
| !!! | TGAAATATAAATGTTTTTAC+CGG | + | chr7.3:6353169-6353188 | None:intergenic | 15.0% |
| !!! | TTATATTTTAATTGAAATGC+AGG | - | chr7.3:6354292-6354311 | MS.gene06980:CDS | 15.0% |
| !! | AAAATAATATATGGTTGGAA+AGG | + | chr7.3:6353801-6353820 | None:intergenic | 20.0% |
| !! | AAGATGTAAAATATAGCAAA+TGG | + | chr7.3:6353029-6353048 | None:intergenic | 20.0% |
| !! | AATAAATTGCAGAAGAAATT+GGG | + | chr7.3:6352842-6352861 | None:intergenic | 20.0% |
| !! | CAATGAAAATAATATATGGT+TGG | + | chr7.3:6353806-6353825 | None:intergenic | 20.0% |
| !! | CCAACAATGAAAATAATATA+TGG | + | chr7.3:6353810-6353829 | None:intergenic | 20.0% |
| !! | GAAAATCTATTAATTTCAGA+TGG | - | chr7.3:6352698-6352717 | MS.gene06980:intron | 20.0% |
| !! | TAATAAATTGCAGAAGAAAT+TGG | + | chr7.3:6352843-6352862 | None:intergenic | 20.0% |
| !! | TTGATATAACTTACCAATAT+AGG | + | chr7.3:6353707-6353726 | None:intergenic | 20.0% |
| !!! | ATAAGACTAATTTTAACAGT+TGG | + | chr7.3:6353226-6353245 | None:intergenic | 20.0% |
| !!! | CCATATATTATTTTCATTGT+TGG | - | chr7.3:6353807-6353826 | MS.gene06980:intron | 20.0% |
| ! | AACTTATTAACAACATTTGG+AGG | - | chr7.3:6354324-6354343 | MS.gene06980:CDS | 25.0% |
| ! | AGTAAAATTTCATTTACGCT+TGG | + | chr7.3:6352665-6352684 | None:intergenic | 25.0% |
| ! | ATAATTGGTTACATGCTTTA+AGG | - | chr7.3:6353674-6353693 | MS.gene06980:intron | 25.0% |
| ! | ATGAGATATATGGAATAGAA+AGG | + | chr7.3:6353492-6353511 | None:intergenic | 25.0% |
| ! | ATTAAGACTTACCTATATTG+AGG | - | chr7.3:6353974-6353993 | MS.gene06980:intron | 25.0% |
| ! | ATTATTCATCTTATTGCATG+AGG | - | chr7.3:6353273-6353292 | MS.gene06980:intron | 25.0% |
| ! | CTCTTCATGAATATTGAATT+AGG | - | chr7.3:6353429-6353448 | MS.gene06980:intron | 25.0% |
| ! | CTTAGTTATGCATAATAGAA+AGG | - | chr7.3:6352984-6353003 | MS.gene06980:intron | 25.0% |
| ! | GCAATTTATTAAGTCATTCT+TGG | - | chr7.3:6352852-6352871 | MS.gene06980:intron | 25.0% |
| ! | GGAAACTTATTAACAACATT+TGG | - | chr7.3:6354321-6354340 | MS.gene06980:CDS | 25.0% |
| ! | TATAGCAAATGGAAAGAATT+AGG | + | chr7.3:6353018-6353037 | None:intergenic | 25.0% |
| ! | TCATATCAAGATGAGATATA+TGG | + | chr7.3:6353502-6353521 | None:intergenic | 25.0% |
| ! | TCATGAATATTGAATTAGGT+AGG | - | chr7.3:6353433-6353452 | MS.gene06980:intron | 25.0% |
| ! | TCTTATATTCAGTAGTGATT+TGG | - | chr7.3:6353195-6353214 | MS.gene06980:intron | 25.0% |
| ! | TGATACTTATGCATCTAATA+TGG | - | chr7.3:6353330-6353349 | MS.gene06980:intron | 25.0% |
| ! | TTATCAGCTATAGATTTGAA+AGG | + | chr7.3:6353737-6353756 | None:intergenic | 25.0% |
| !! | ATATTATTTTCATTGTTGGC+AGG | - | chr7.3:6353811-6353830 | MS.gene06980:intron | 25.0% |
| !!! | CAGTTTTGGTGTTATAAAAA+TGG | + | chr7.3:6354049-6354068 | None:intergenic | 25.0% |
| !!! | GTTATTGGTTTGTTAAGTTT+TGG | - | chr7.3:6354193-6354212 | MS.gene06980:intron | 25.0% |
| !!! | TATGGAGATTTGTTGATAAT+TGG | - | chr7.3:6353659-6353678 | MS.gene06980:intron | 25.0% |
| AAGGAATTTCAAGGAAATTG+AGG | - | chr7.3:6354171-6354190 | MS.gene06980:intron | 30.0% | |
| AATTGTCTTCAACGTTGTAA+CGG | + | chr7.3:6353851-6353870 | None:intergenic | 30.0% | |
| AGCTAATAAAGAACACTTAC+CGG | - | chr7.3:6353147-6353166 | MS.gene06980:intron | 30.0% | |
| CTTTGACAATTGCTTATCTA+TGG | - | chr7.3:6353382-6353401 | MS.gene06980:intron | 30.0% | |
| GCTCAAATGTTATCATCATT+TGG | + | chr7.3:6352551-6352570 | None:intergenic | 30.0% | |
| TAACCATAGAACCTCAATAT+AGG | + | chr7.3:6353988-6354007 | None:intergenic | 30.0% | |
| TAATTGAAATGCAGGTCATA+TGG | - | chr7.3:6354300-6354319 | MS.gene06980:CDS | 30.0% | |
| TATGCATAACTAAGAATTGC+TGG | + | chr7.3:6352978-6352997 | None:intergenic | 30.0% | |
| TATTTGTAGTCTGCTAATAG+TGG | + | chr7.3:6353887-6353906 | None:intergenic | 30.0% | |
| TCCAGAAAACACTATATGTT+AGG | - | chr7.3:6354101-6354120 | MS.gene06980:intron | 30.0% | |
| TCTATAGCTGATAAATCTTC+AGG | - | chr7.3:6353743-6353762 | MS.gene06980:intron | 30.0% | |
| TCTGTGAAAAGTTCTACTTA+TGG | - | chr7.3:6352940-6352959 | MS.gene06980:intron | 30.0% | |
| TGGATCAAATATAAAATGCC+TGG | + | chr7.3:6354085-6354104 | None:intergenic | 30.0% | |
| TTACCTATATTGAGGTTCTA+TGG | - | chr7.3:6353982-6354001 | MS.gene06980:intron | 30.0% | |
| TTCAAGGAAATTGAGGTTAT+TGG | - | chr7.3:6354178-6354197 | MS.gene06980:intron | 30.0% | |
| ! | ACTTTGAAGATGATTTCATG+AGG | - | chr7.3:6353906-6353925 | MS.gene06980:intron | 30.0% |
| ! | TTTTATAACACCAAAACTGC+TGG | - | chr7.3:6354050-6354069 | MS.gene06980:intron | 30.0% |
| !! | CTAATAGTGGCTTTAATAGT+TGG | + | chr7.3:6353874-6353893 | None:intergenic | 30.0% |
| !!! | AGACTTTTACATTTGCTGTT+AGG | + | chr7.3:6353364-6353383 | None:intergenic | 30.0% |
| !!! | AGGATTTTAATTGTTGTAGC+AGG | + | chr7.3:6353781-6353800 | None:intergenic | 30.0% |
| AACTTACCAATATAGGATCG+CGG | + | chr7.3:6353700-6353719 | None:intergenic | 35.0% | |
| AATACATGCACAACCATGAA+TGG | + | chr7.3:6353957-6353976 | None:intergenic | 35.0% | |
| AGGACATGATGTGAACAAAT+TGG | + | chr7.3:6353472-6353491 | None:intergenic | 35.0% | |
| AGGTCATACATCAAAATCTG+TGG | + | chr7.3:6353570-6353589 | None:intergenic | 35.0% | |
| AGTCATTCTTGGATAAGCAA+TGG | - | chr7.3:6352863-6352882 | MS.gene06980:intron | 35.0% | |
| ATACATGCACAACCATGAAT+GGG | + | chr7.3:6353956-6353975 | None:intergenic | 35.0% | |
| ATTGTCTTCAACGTTGTAAC+GGG | + | chr7.3:6353850-6353869 | None:intergenic | 35.0% | |
| CATGAGGAATGTATTGTATG+TGG | - | chr7.3:6353289-6353308 | MS.gene06980:intron | 35.0% | |
| GAAAAAAAATGAGCAGCTTC+AGG | + | chr7.3:6352818-6352837 | None:intergenic | 35.0% | |
| GGAATTTGATCCAAAACAGA+AGG | + | chr7.3:6352615-6352634 | None:intergenic | 35.0% | |
| GGACATGATGTGAACAAATT+GGG | + | chr7.3:6353471-6353490 | None:intergenic | 35.0% | |
| TACTGTTGGAAGGAATTTCA+AGG | - | chr7.3:6354162-6354181 | MS.gene06980:intron | 35.0% | |
| TTGAAGATGATTTCATGAGG+TGG | - | chr7.3:6353909-6353928 | MS.gene06980:CDS | 35.0% | |
| ! | GCCTAACATATAGTGTTTTC+TGG | + | chr7.3:6354105-6354124 | None:intergenic | 35.0% |
| ! | GTTCTATGGTTAGTCATTGT+TGG | - | chr7.3:6353996-6354015 | MS.gene06980:intron | 35.0% |
| ! | TGAGAAGCACATAAAAGAGT+TGG | + | chr7.3:6352779-6352798 | None:intergenic | 35.0% |
| !!! | TTGGTTTGTTAAGTTTTGGC+AGG | - | chr7.3:6354197-6354216 | MS.gene06980:intron | 35.0% |
| AAATCTCCATAGCTGCTGTT+GGG | + | chr7.3:6353650-6353669 | None:intergenic | 40.0% | |
| AACTATGCTGCTGATACTGT+TGG | - | chr7.3:6354148-6354167 | MS.gene06980:intron | 40.0% | |
| ATAACACCAAAACTGCTGGT+TGG | - | chr7.3:6354054-6354073 | MS.gene06980:intron | 40.0% | |
| CTTCTTCTTCATTGCAAAGC+TGG | + | chr7.3:6353096-6353115 | None:intergenic | 40.0% | |
| CTTGGTCAAGTTCATGAGAA+TGG | + | chr7.3:6352647-6352666 | None:intergenic | 40.0% | |
| GATGATAACATTTGAGCAGC+TGG | - | chr7.3:6352554-6352573 | MS.gene06980:CDS | 40.0% | |
| GCTTTGCAATGAAGAAGAAG+AGG | - | chr7.3:6353096-6353115 | MS.gene06980:intron | 40.0% | |
| GGATAAGCAATGGTTGTTCA+AGG | - | chr7.3:6352873-6352892 | MS.gene06980:intron | 40.0% | |
| TAGGATATGATGCCCATTCA+TGG | - | chr7.3:6353941-6353960 | MS.gene06980:CDS | 40.0% | |
| TCAAGTTCATGAGAATGGCA+TGG | + | chr7.3:6352642-6352661 | None:intergenic | 40.0% | |
| TGATCCAAAACAGAAGGCAA+CGG | + | chr7.3:6352609-6352628 | None:intergenic | 40.0% | |
| TGGTTGTTCAAGGTTGAAGT+TGG | - | chr7.3:6352883-6352902 | MS.gene06980:intron | 40.0% | |
| !! | TTCAAGGTTGAAGTTGGTGA+TGG | - | chr7.3:6352889-6352908 | MS.gene06980:intron | 40.0% |
| ACATATCCCAACAGCAGCTA+TGG | - | chr7.3:6353641-6353660 | MS.gene06980:intron | 45.0% | |
| AGAAGGCAACGGTGATTCTT+GGG | + | chr7.3:6352598-6352617 | None:intergenic | 45.0% | |
| AGCTGCTGTTGGGATATGTT+TGG | + | chr7.3:6353640-6353659 | None:intergenic | 45.0% | |
| ATGCTGCTGATACTGTTGGA+AGG | - | chr7.3:6354152-6354171 | MS.gene06980:intron | 45.0% | |
| CAAATCTCCATAGCTGCTGT+TGG | + | chr7.3:6353651-6353670 | None:intergenic | 45.0% | |
| CATCATTTGGATCCAGCCAA+TGG | + | chr7.3:6352538-6352557 | None:intergenic | 45.0% | |
| CATGAGGTGGAAACAGATGT+AGG | - | chr7.3:6353922-6353941 | MS.gene06980:CDS | 45.0% | |
| GTACTCAACACATTGCAGCA+AGG | + | chr7.3:6353590-6353609 | None:intergenic | 45.0% | |
| TATGTGCTTCTCAGCAGAAG+AGG | - | chr7.3:6352786-6352805 | MS.gene06980:CDS | 45.0% | |
| TCATGAGAATGGCATGGAGA+AGG | + | chr7.3:6352636-6352655 | None:intergenic | 45.0% | |
| TTAAGGCCGCGATCCTATAT+TGG | - | chr7.3:6353691-6353710 | MS.gene06980:intron | 45.0% | |
| ! | ATCACCGTTGCCTTCTGTTT+TGG | - | chr7.3:6352602-6352621 | MS.gene06980:intron | 45.0% |
| CAGAAGGCAACGGTGATTCT+TGG | + | chr7.3:6352599-6352618 | None:intergenic | 50.0% | |
| GAAGGCAACGGTGATTCTTG+GGG | + | chr7.3:6352597-6352616 | None:intergenic | 50.0% | |
| GCAGCATAGTTCTTACGCAG+TGG | + | chr7.3:6354139-6354158 | None:intergenic | 50.0% | |
| ! | GACACACCAACCAGCAGTTT+TGG | + | chr7.3:6354063-6354082 | None:intergenic | 50.0% |
| ! | AACTGCTGGTTGGTGTGTCC+AGG | - | chr7.3:6354064-6354083 | MS.gene06980:intron | 55.0% |
| GCAACGGTGATTCTTGGGGC+TGG | + | chr7.3:6352593-6352612 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.3 | gene | 6352525 | 6354388 | 6352525 | ID=MS.gene06980 |
| chr7.3 | mRNA | 6352525 | 6354388 | 6352525 | ID=MS.gene06980.t1;Parent=MS.gene06980 |
| chr7.3 | exon | 6354246 | 6354388 | 6354246 | ID=MS.gene06980.t1.exon1;Parent=MS.gene06980.t1 |
| chr7.3 | CDS | 6354246 | 6354388 | 6354246 | ID=cds.MS.gene06980.t1;Parent=MS.gene06980.t1 |
| chr7.3 | exon | 6353908 | 6353962 | 6353908 | ID=MS.gene06980.t1.exon2;Parent=MS.gene06980.t1 |
| chr7.3 | CDS | 6353908 | 6353962 | 6353908 | ID=cds.MS.gene06980.t1;Parent=MS.gene06980.t1 |
| chr7.3 | exon | 6353745 | 6353816 | 6353745 | ID=MS.gene06980.t1.exon3;Parent=MS.gene06980.t1 |
| chr7.3 | CDS | 6353745 | 6353816 | 6353745 | ID=cds.MS.gene06980.t1;Parent=MS.gene06980.t1 |
| chr7.3 | exon | 6353201 | 6353258 | 6353201 | ID=MS.gene06980.t1.exon4;Parent=MS.gene06980.t1 |
| chr7.3 | CDS | 6353201 | 6353258 | 6353201 | ID=cds.MS.gene06980.t1;Parent=MS.gene06980.t1 |
| chr7.3 | exon | 6352721 | 6352827 | 6352721 | ID=MS.gene06980.t1.exon5;Parent=MS.gene06980.t1 |
| chr7.3 | CDS | 6352721 | 6352827 | 6352721 | ID=cds.MS.gene06980.t1;Parent=MS.gene06980.t1 |
| chr7.3 | exon | 6352525 | 6352599 | 6352525 | ID=MS.gene06980.t1.exon6;Parent=MS.gene06980.t1 |
| chr7.3 | CDS | 6352525 | 6352599 | 6352525 | ID=cds.MS.gene06980.t1;Parent=MS.gene06980.t1 |
| Gene Sequence |
| Protein sequence |
