Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene06987.t1 | XP_003625923.1 | 97.7 | 309 | 1 | 3 | 1 | 308 | 1 | 304 | 1.30E-159 | 572.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene06987.t1 | Q8LEA8 | 46.3 | 337 | 145 | 7 | 1 | 307 | 5 | 335 | 3.8e-80 | 299.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene06987.t1 | G7KRX0 | 97.7 | 309 | 1 | 3 | 1 | 308 | 1 | 304 | 9.2e-160 | 572.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene05316 | MS.gene06987 | 0.806557 | 7.76E-50 | -1.69E-46 |
| MS.gene057668 | MS.gene06987 | 0.828311 | 9.65E-55 | -1.69E-46 |
| MS.gene059807 | MS.gene06987 | 0.835897 | 1.28E-56 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene06987.t1 | MTR_7g108820 | 96.764 | 309 | 4 | 3 | 1 | 308 | 1 | 304 | 0.0 | 615 |
| MS.gene06987.t1 | MTR_8g058520 | 96.764 | 309 | 4 | 3 | 1 | 308 | 1 | 304 | 0.0 | 615 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene06987.t1 | AT4G02440 | 46.884 | 337 | 143 | 6 | 1 | 307 | 5 | 335 | 4.21e-105 | 310 |
Find 56 sgRNAs with CRISPR-Local
Find 62 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGTGATAATGGTCATGTTTC+TGG | 0.130245 | 7.3:+6301647 | MS.gene06987:CDS |
| CAAATGAATGTTCTTCTTAT+TGG | 0.137353 | 7.3:+6301120 | MS.gene06987:CDS |
| AGGGCTTATGTTTGTGATAA+TGG | 0.254503 | 7.3:+6301635 | MS.gene06987:CDS |
| CACATTTAGCTACAGGAAAA+TGG | 0.257698 | 7.3:+6301312 | MS.gene06987:CDS |
| ACCTTTCTCTTCTTAGCTTC+TGG | 0.278200 | 7.3:-6301272 | None:intergenic |
| GTTGTTCTTGTCCCTGTTGA+AGG | 0.305088 | 7.3:-6301199 | None:intergenic |
| TGTTGGTGTTGTTGTTCATC+TGG | 0.306138 | 7.3:-6301035 | None:intergenic |
| GCTAAGAAGAGAAAGGTTAA+TGG | 0.338615 | 7.3:+6301278 | MS.gene06987:CDS |
| GTTTATGAAGAGAAAGATCT+TGG | 0.359162 | 7.3:-6300953 | None:intergenic |
| GTTGTTGATGATGATGTAAT+AGG | 0.379805 | 7.3:-6301059 | None:intergenic |
| TTTGTTCTTGTAATCATACT+TGG | 0.383121 | 7.3:+6301546 | MS.gene06987:CDS |
| TAATCGGAAGAAGATTGATT+TGG | 0.384060 | 7.3:+6301514 | MS.gene06987:CDS |
| ATGATGGTGAACCTGTTGTT+AGG | 0.390801 | 7.3:+6301615 | MS.gene06987:CDS |
| ATGGTCATGTTTCTGGTGCT+TGG | 0.396836 | 7.3:+6301654 | MS.gene06987:CDS |
| TTGAAGAAGACCCTCGTGAT+TGG | 0.397252 | 7.3:+6300823 | MS.gene06987:CDS |
| TTGGCCAGGTTGTGTTCATT+TGG | 0.410463 | 7.3:+6301406 | MS.gene06987:CDS |
| CTAAGAAGAGAAAGGTTAAT+GGG | 0.420309 | 7.3:+6301279 | MS.gene06987:CDS |
| TGAAGCACATCACCTTCAAC+AGG | 0.422074 | 7.3:+6301187 | MS.gene06987:CDS |
| TGTTCTTCCAACAGAAATCA+TGG | 0.422948 | 7.3:-6300887 | None:intergenic |
| CTCTCATCCATGATTTCTGT+TGG | 0.424893 | 7.3:+6300880 | MS.gene06987:CDS |
| ATTGTTTATACGTATGTGAT+TGG | 0.431379 | 7.3:+6301387 | MS.gene06987:CDS |
| ATTTCAAGAAGACACGTGTT+TGG | 0.435090 | 7.3:+6301471 | MS.gene06987:CDS |
| TTGAGTTCACATTTAGCTAC+AGG | 0.438504 | 7.3:+6301305 | MS.gene06987:CDS |
| GAAGAAAGAAGAAGTTCAGA+AGG | 0.446221 | 7.3:-6300927 | None:intergenic |
| TTGTTCTTGTAATCATACTT+GGG | 0.459848 | 7.3:+6301547 | MS.gene06987:CDS |
| AAACTACCCTTCTGTTGTCC+TGG | 0.461163 | 7.3:+6300972 | MS.gene06987:CDS |
| CTGTGAATGATATTAGTAAT+CGG | 0.469652 | 7.3:+6301498 | MS.gene06987:CDS |
| AGTATGATTACAAGAACAAA+AGG | 0.477331 | 7.3:-6301543 | None:intergenic |
| TGAAGAAGACCCTCGTGATT+GGG | 0.483756 | 7.3:+6300824 | MS.gene06987:CDS |
| GAAGAACATTCATTTGTTTG+AGG | 0.484296 | 7.3:-6301113 | None:intergenic |
| TTTCTCTTCTTAGCTTCTGG+TGG | 0.489673 | 7.3:-6301269 | None:intergenic |
| GAACATTCATTTGTTTGAGG+TGG | 0.490824 | 7.3:-6301110 | None:intergenic |
| GTTGGTGTTGTTGTTCATCT+GGG | 0.491428 | 7.3:-6301034 | None:intergenic |
| CAAACATAAGCCCTAACAAC+AGG | 0.496612 | 7.3:-6301626 | None:intergenic |
| ATGATGATGTAATAGGTTGT+TGG | 0.497159 | 7.3:-6301052 | None:intergenic |
| AACGAGGAAGAGATTAGAAC+TGG | 0.503126 | 7.3:+6301233 | MS.gene06987:CDS |
| TGTCCTGGTCCTCTTCATGC+TGG | 0.523403 | 7.3:+6300987 | MS.gene06987:CDS |
| TTTATGAAGAGAAAGATCTT+GGG | 0.527438 | 7.3:-6300952 | None:intergenic |
| ACCAGAAGCTAAGAAGAGAA+AGG | 0.540167 | 7.3:+6301271 | MS.gene06987:CDS |
| GGATTTGGTTATCATGATGA+TGG | 0.549376 | 7.3:+6301599 | MS.gene06987:CDS |
| AGTTCTAATCTCTTCCTCGT+TGG | 0.559801 | 7.3:-6301231 | None:intergenic |
| AAGAGGACCAGGACAACAGA+AGG | 0.566286 | 7.3:-6300979 | None:intergenic |
| AAGAATGATCAAGCGTACAT+AGG | 0.570141 | 7.3:-6301686 | None:intergenic |
| TGATGGTGAACCTGTTGTTA+GGG | 0.575949 | 7.3:+6301616 | MS.gene06987:CDS |
| GAAAGACGAGCCCAATCACG+AGG | 0.597495 | 7.3:-6300834 | None:intergenic |
| ATACCAGCATGAAGAGGACC+AGG | 0.606219 | 7.3:-6300990 | None:intergenic |
| AAGAGAATACCAGCATGAAG+AGG | 0.613643 | 7.3:-6300996 | None:intergenic |
| TCTTCCAAATGAACACAACC+TGG | 0.613786 | 7.3:-6301410 | None:intergenic |
| TGGACTCTTAGTAGAGAACA+AGG | 0.622079 | 7.3:+6301332 | MS.gene06987:CDS |
| TGAGGTGGAAGAGATGATGA+AGG | 0.629257 | 7.3:-6301095 | None:intergenic |
| TTGTTCATCTGGGAAATCAG+CGG | 0.640290 | 7.3:-6301024 | None:intergenic |
| AGAGGACCAGGACAACAGAA+GGG | 0.650940 | 7.3:-6300978 | None:intergenic |
| TTATACGTATGTGATTGGCC+AGG | 0.658255 | 7.3:+6301392 | MS.gene06987:CDS |
| GAAGCACATCACCTTCAACA+GGG | 0.717553 | 7.3:+6301188 | MS.gene06987:CDS |
| ACAACAAGAACAGACCAACG+AGG | 0.731842 | 7.3:+6301217 | MS.gene06987:CDS |
| AAAGACGAGCCCAATCACGA+GGG | 0.734594 | 7.3:-6300833 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATATAATTCTGAAGATTTTA+TGG | + | chr7.3:6300796-6300815 | MS.gene06987:CDS | 15.0% |
| ! | AGTATGATTACAAGAACAAA+AGG | - | chr7.3:6301546-6301565 | None:intergenic | 25.0% |
| ! | ATTGTTTATACGTATGTGAT+TGG | + | chr7.3:6301387-6301406 | MS.gene06987:CDS | 25.0% |
| ! | CAAATGAATGTTCTTCTTAT+TGG | + | chr7.3:6301120-6301139 | MS.gene06987:CDS | 25.0% |
| ! | CTGTGAATGATATTAGTAAT+CGG | + | chr7.3:6301498-6301517 | MS.gene06987:CDS | 25.0% |
| ! | TTGTTCTTGTAATCATACTT+GGG | + | chr7.3:6301547-6301566 | MS.gene06987:CDS | 25.0% |
| ! | TTTATGAAGAGAAAGATCTT+GGG | - | chr7.3:6300955-6300974 | None:intergenic | 25.0% |
| ! | TTTGTTCTTGTAATCATACT+TGG | + | chr7.3:6301546-6301565 | MS.gene06987:CDS | 25.0% |
| !!! | AATTCTGAAGATTTTATGGA+AGG | + | chr7.3:6300800-6300819 | MS.gene06987:CDS | 25.0% |
| CTAAGAAGAGAAAGGTTAAT+GGG | + | chr7.3:6301279-6301298 | MS.gene06987:CDS | 30.0% | |
| GAAGAACATTCATTTGTTTG+AGG | - | chr7.3:6301116-6301135 | None:intergenic | 30.0% | |
| GTTTATGAAGAGAAAGATCT+TGG | - | chr7.3:6300956-6300975 | None:intergenic | 30.0% | |
| TAATCGGAAGAAGATTGATT+TGG | + | chr7.3:6301514-6301533 | MS.gene06987:CDS | 30.0% | |
| ! | ATGATGATGTAATAGGTTGT+TGG | - | chr7.3:6301055-6301074 | None:intergenic | 30.0% |
| !! | GTTGTTGATGATGATGTAAT+AGG | - | chr7.3:6301062-6301081 | None:intergenic | 30.0% |
| !!! | AGAAAGTATAGACTTTTTCG+AGG | + | chr7.3:6301437-6301456 | MS.gene06987:CDS | 30.0% |
| AGGGCTTATGTTTGTGATAA+TGG | + | chr7.3:6301635-6301654 | MS.gene06987:CDS | 35.0% | |
| ATTTCAAGAAGACACGTGTT+TGG | + | chr7.3:6301471-6301490 | MS.gene06987:CDS | 35.0% | |
| CACATTTAGCTACAGGAAAA+TGG | + | chr7.3:6301312-6301331 | MS.gene06987:CDS | 35.0% | |
| GAACATTCATTTGTTTGAGG+TGG | - | chr7.3:6301113-6301132 | None:intergenic | 35.0% | |
| GAAGAAAGAAGAAGTTCAGA+AGG | - | chr7.3:6300930-6300949 | None:intergenic | 35.0% | |
| GCTAAGAAGAGAAAGGTTAA+TGG | + | chr7.3:6301278-6301297 | MS.gene06987:CDS | 35.0% | |
| TGTTCTTCCAACAGAAATCA+TGG | - | chr7.3:6300890-6300909 | None:intergenic | 35.0% | |
| TTGAGTTCACATTTAGCTAC+AGG | + | chr7.3:6301305-6301324 | MS.gene06987:CDS | 35.0% | |
| ! | GATGAGAGAATTGAGTTTTG+TGG | - | chr7.3:6300868-6300887 | None:intergenic | 35.0% |
| ! | GGATTTGGTTATCATGATGA+TGG | + | chr7.3:6301599-6301618 | MS.gene06987:CDS | 35.0% |
| !! | TGTGATAATGGTCATGTTTC+TGG | + | chr7.3:6301647-6301666 | MS.gene06987:CDS | 35.0% |
| !! | TTTTGTTTGAGGAGAGGATT+TGG | + | chr7.3:6301584-6301603 | MS.gene06987:CDS | 35.0% |
| !!! | TTCACTCTGCTTTTTGTTTG+AGG | + | chr7.3:6301573-6301592 | MS.gene06987:CDS | 35.0% |
| AACGAGGAAGAGATTAGAAC+TGG | + | chr7.3:6301233-6301252 | MS.gene06987:CDS | 40.0% | |
| AAGAGAATACCAGCATGAAG+AGG | - | chr7.3:6300999-6301018 | None:intergenic | 40.0% | |
| ACCAGAAGCTAAGAAGAGAA+AGG | + | chr7.3:6301271-6301290 | MS.gene06987:CDS | 40.0% | |
| ACCTTTCTCTTCTTAGCTTC+TGG | - | chr7.3:6301275-6301294 | None:intergenic | 40.0% | |
| CAAACATAAGCCCTAACAAC+AGG | - | chr7.3:6301629-6301648 | None:intergenic | 40.0% | |
| CTCTCATCCATGATTTCTGT+TGG | + | chr7.3:6300880-6300899 | MS.gene06987:CDS | 40.0% | |
| TCTTCCAAATGAACACAACC+TGG | - | chr7.3:6301413-6301432 | None:intergenic | 40.0% | |
| TGGACTCTTAGTAGAGAACA+AGG | + | chr7.3:6301332-6301351 | MS.gene06987:CDS | 40.0% | |
| TTGTTCATCTGGGAAATCAG+CGG | - | chr7.3:6301027-6301046 | None:intergenic | 40.0% | |
| TTTCTCTTCTTAGCTTCTGG+TGG | - | chr7.3:6301272-6301291 | None:intergenic | 40.0% | |
| ! | AGTTCTAATCTCTTCCTCGT+TGG | - | chr7.3:6301234-6301253 | None:intergenic | 40.0% |
| ! | ATGATGGTGAACCTGTTGTT+AGG | + | chr7.3:6301615-6301634 | MS.gene06987:CDS | 40.0% |
| ! | TGATGGTGAACCTGTTGTTA+GGG | + | chr7.3:6301616-6301635 | MS.gene06987:CDS | 40.0% |
| ! | TTATACGTATGTGATTGGCC+AGG | + | chr7.3:6301392-6301411 | MS.gene06987:CDS | 40.0% |
| !! | GTTGGTGTTGTTGTTCATCT+GGG | - | chr7.3:6301037-6301056 | None:intergenic | 40.0% |
| !! | TGTTGGTGTTGTTGTTCATC+TGG | - | chr7.3:6301038-6301057 | None:intergenic | 40.0% |
| !!! | TCTGCTTTTTGTTTGAGGAG+AGG | + | chr7.3:6301578-6301597 | MS.gene06987:CDS | 40.0% |
| AAACTACCCTTCTGTTGTCC+TGG | + | chr7.3:6300972-6300991 | MS.gene06987:CDS | 45.0% | |
| ACAACAAGAACAGACCAACG+AGG | + | chr7.3:6301217-6301236 | MS.gene06987:CDS | 45.0% | |
| GAAGCACATCACCTTCAACA+GGG | + | chr7.3:6301188-6301207 | MS.gene06987:CDS | 45.0% | |
| GTTGTTCTTGTCCCTGTTGA+AGG | - | chr7.3:6301202-6301221 | None:intergenic | 45.0% | |
| TGAAGAAGACCCTCGTGATT+GGG | + | chr7.3:6300824-6300843 | MS.gene06987:CDS | 45.0% | |
| TGAAGCACATCACCTTCAAC+AGG | + | chr7.3:6301187-6301206 | MS.gene06987:CDS | 45.0% | |
| TGAGGTGGAAGAGATGATGA+AGG | - | chr7.3:6301098-6301117 | None:intergenic | 45.0% | |
| TTGAAGAAGACCCTCGTGAT+TGG | + | chr7.3:6300823-6300842 | MS.gene06987:CDS | 45.0% | |
| TTGGCCAGGTTGTGTTCATT+TGG | + | chr7.3:6301406-6301425 | MS.gene06987:CDS | 45.0% | |
| !! | ATGGTCATGTTTCTGGTGCT+TGG | + | chr7.3:6301654-6301673 | MS.gene06987:CDS | 45.0% |
| AAAGACGAGCCCAATCACGA+GGG | - | chr7.3:6300836-6300855 | None:intergenic | 50.0% | |
| AAGAGGACCAGGACAACAGA+AGG | - | chr7.3:6300982-6301001 | None:intergenic | 50.0% | |
| AGAGGACCAGGACAACAGAA+GGG | - | chr7.3:6300981-6301000 | None:intergenic | 50.0% | |
| ATACCAGCATGAAGAGGACC+AGG | - | chr7.3:6300993-6301012 | None:intergenic | 50.0% | |
| GAAAGACGAGCCCAATCACG+AGG | - | chr7.3:6300837-6300856 | None:intergenic | 55.0% | |
| TGTCCTGGTCCTCTTCATGC+TGG | + | chr7.3:6300987-6301006 | MS.gene06987:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.3 | gene | 6300774 | 6301700 | 6300774 | ID=MS.gene06987 |
| chr7.3 | mRNA | 6300774 | 6301700 | 6300774 | ID=MS.gene06987.t1;Parent=MS.gene06987 |
| chr7.3 | exon | 6300774 | 6301700 | 6300774 | ID=MS.gene06987.t1.exon1;Parent=MS.gene06987.t1 |
| chr7.3 | CDS | 6300774 | 6301700 | 6300774 | ID=cds.MS.gene06987.t1;Parent=MS.gene06987.t1 |
| Gene Sequence |
| Protein sequence |
