AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene070099


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene070099.t1 MTR_5g027000 94.375 160 9 0 1 160 1 160 7.73e-106 299
MS.gene070099.t1 MTR_8g105230 42.748 131 75 0 23 153 37 167 2.01e-29 106
MS.gene070099.t1 MTR_1g032070 38.168 131 81 0 23 153 47 177 1.27e-25 97.1
MS.gene070099.t1 MTR_1g076650 35.821 134 86 0 19 152 9 142 4.51e-24 91.7
MS.gene070099.t1 MTR_6g025320 34.307 137 90 0 16 152 6 142 7.61e-24 91.3
MS.gene070099.t1 MTR_5g088320 34.307 137 90 0 16 152 6 142 1.25e-23 90.5
MS.gene070099.t1 MTR_7g087610 34.307 137 90 0 16 152 6 142 4.18e-23 89.4
MS.gene070099.t1 MTR_8g066630 33.566 143 92 1 19 158 14 156 6.21e-23 89.4
MS.gene070099.t1 MTR_1g041285 33.333 135 90 0 18 152 8 142 1.37e-21 85.5
MS.gene070099.t1 MTR_3g067610 35.385 130 80 1 27 156 10 135 8.47e-20 80.5
MS.gene070099.t1 MTR_8g107110 32.061 131 88 1 23 153 25 154 1.31e-18 78.2
MS.gene070099.t1 MTR_5g079340 32.061 131 86 2 27 156 9 137 6.38e-18 75.5
MS.gene070099.t1 MTR_4g112460 31.973 147 95 2 12 156 4 147 2.95e-17 74.7
MS.gene070099.t1 MTR_8g069915 31.034 145 98 1 14 156 4 148 3.39e-17 74.7
MS.gene070099.t1 MTR_1g046950 32.331 133 88 1 23 153 5 137 8.42e-17 72.8
MS.gene070099.t1 MTR_6g079570 30.216 139 95 2 23 160 27 164 1.19e-16 73.2
MS.gene070099.t1 MTR_7g089760 31.884 138 92 1 23 158 6 143 1.69e-16 72.4
MS.gene070099.t1 MTR_6g023460 28.369 141 100 1 16 156 2 141 4.68e-16 70.9
MS.gene070099.t1 MTR_1g047100 29.927 137 95 1 23 158 5 141 5.15e-16 70.9
MS.gene070099.t1 MTR_5g011850 28.889 135 94 1 18 152 8 140 5.24e-16 70.9
MS.gene070099.t1 MTR_4g067270 31.579 133 89 1 23 153 6 138 8.21e-16 70.5
MS.gene070099.t1 MTR_4g103655 32.031 128 86 1 30 157 14 140 3.56e-15 68.6
MS.gene070099.t1 MTR_2g098890 30.370 135 90 2 23 153 65 199 3.95e-15 70.1
MS.gene070099.t1 MTR_4g086260 31.250 128 84 2 26 152 61 185 6.59e-15 68.9
MS.gene070099.t1 MTR_4g103630 32.031 128 86 1 30 157 14 140 1.20e-14 67.0
MS.gene070099.t1 MTR_4g103650 32.031 128 86 1 30 157 10 136 2.16e-14 66.2
MS.gene070099.t1 MTR_7g115040 29.104 134 95 0 19 152 9 142 2.31e-14 66.6
MS.gene070099.t1 MTR_3g083470 31.538 130 79 1 27 156 98 217 1.65e-13 65.9
MS.gene070099.t1 MTR_1g071150 29.927 137 95 1 16 152 6 141 1.91e-13 64.3
MS.gene070099.t1 MTR_8g036075 32.394 142 82 4 23 153 50 188 3.27e-13 64.7
MS.gene070099.t1 MTR_0168s0120 30.189 106 74 0 47 152 1 106 4.77e-13 62.4
MS.gene070099.t1 MTR_6g025320 30.189 106 74 0 47 152 1 106 4.77e-13 62.4
MS.gene070099.t1 MTR_8g099095 27.815 151 107 2 2 152 351 499 5.83e-13 65.9
MS.gene070099.t1 MTR_5g009830 27.815 151 107 2 2 152 351 499 9.80e-13 65.1
MS.gene070099.t1 MTR_7g075040 32.484 157 92 6 12 158 40 192 7.17e-12 60.8
MS.gene070099.t1 MTR_3g089070 29.600 125 87 1 28 152 12 135 1.19e-11 59.3
MS.gene070099.t1 MTR_3g089090 29.600 125 87 1 28 152 11 134 1.31e-11 59.3
MS.gene070099.t1 MTR_7g090450 30.201 149 95 4 16 157 67 213 1.61e-11 60.5
MS.gene070099.t1 MTR_7g074020 25.547 137 102 0 16 152 24 160 3.96e-11 58.5
MS.gene070099.t1 MTR_0028s0170 28.358 134 94 2 19 152 380 511 4.26e-11 60.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene070099.t1 AT1G24620 34.000 150 99 0 7 156 22 171 7.28e-27 100
MS.gene070099.t1 AT5G37780 35.461 141 91 0 16 156 6 146 1.42e-24 93.2
MS.gene070099.t1 AT1G66410 35.461 141 91 0 16 156 6 146 1.42e-24 93.2
MS.gene070099.t1 AT5G21274 35.036 137 89 0 16 152 6 142 3.83e-24 92.0
MS.gene070099.t1 AT4G14640 35.766 137 88 0 16 152 7 143 4.49e-24 92.0
MS.gene070099.t1 AT3G56800 34.307 137 90 0 16 152 6 142 7.64e-24 91.3
MS.gene070099.t1 AT2G27030 34.307 137 90 0 16 152 6 142 7.64e-24 91.3
MS.gene070099.t1 AT2G41110 34.307 137 90 0 16 152 6 142 7.64e-24 91.3
MS.gene070099.t1 AT3G43810 34.307 137 90 0 16 152 6 142 7.97e-24 91.3
MS.gene070099.t1 AT2G27030 34.307 137 90 0 16 152 6 142 1.44e-23 91.3
MS.gene070099.t1 AT3G43810 34.307 137 90 0 16 152 6 142 2.17e-23 91.7
MS.gene070099.t1 AT3G22930 35.766 137 88 0 16 152 29 165 3.51e-23 90.1
MS.gene070099.t1 AT2G41110 31.544 149 90 1 16 152 6 154 2.20e-20 82.8
MS.gene070099.t1 AT5G37780 32.051 156 91 1 16 156 6 161 2.66e-20 82.4
MS.gene070099.t1 AT1G66400 31.034 145 98 1 11 153 4 148 1.88e-19 80.1
MS.gene070099.t1 AT1G18210 29.771 131 88 1 23 153 24 150 2.50e-19 80.1
MS.gene070099.t1 AT1G18210 29.771 131 88 1 23 153 24 150 2.50e-19 80.1
MS.gene070099.t1 AT5G37780 29.940 167 91 1 16 156 6 172 9.81e-19 78.6
MS.gene070099.t1 AT1G66410 33.884 121 80 0 36 156 36 156 1.36e-18 77.8
MS.gene070099.t1 AT5G37780 33.884 121 80 0 36 156 13 133 1.53e-18 77.4
MS.gene070099.t1 AT5G37770 32.237 152 99 1 6 153 1 152 2.30e-18 77.4
MS.gene070099.t1 AT5G37770 32.237 152 99 1 6 153 1 152 2.30e-18 77.4
MS.gene070099.t1 AT4G14640 34.188 117 77 0 36 152 9 125 3.10e-18 76.3
MS.gene070099.t1 AT1G32250 28.767 146 98 1 19 158 13 158 3.51e-17 74.3
MS.gene070099.t1 AT1G05990 34.058 138 88 2 23 157 6 143 8.75e-17 73.2
MS.gene070099.t1 AT1G73630 26.282 156 111 1 3 158 1 152 1.27e-16 72.8
MS.gene070099.t1 AT3G03000 34.043 141 92 1 19 158 17 157 2.04e-16 72.4
MS.gene070099.t1 AT4G12860 31.387 137 92 1 23 157 6 142 1.10e-15 70.1
MS.gene070099.t1 AT3G59440 34.459 148 93 3 13 158 43 188 2.49e-15 70.1
MS.gene070099.t1 AT1G62820 29.630 135 93 1 18 152 9 141 6.12e-15 68.2
MS.gene070099.t1 AT1G12310 29.630 135 93 1 18 152 9 141 2.28e-14 66.6
MS.gene070099.t1 AT4G03290 32.624 141 90 2 23 158 6 146 2.42e-14 66.6
MS.gene070099.t1 AT1G66410 31.818 110 75 0 47 156 1 110 6.21e-14 64.7
MS.gene070099.t1 AT1G66410 31.818 110 75 0 47 156 1 110 6.21e-14 64.7
MS.gene070099.t1 AT3G50360 27.737 137 99 0 16 152 21 157 6.26e-14 65.9
MS.gene070099.t1 AT3G51920 28.467 137 98 0 16 152 6 142 6.34e-14 65.5
MS.gene070099.t1 AT4G37010 28.467 137 98 0 16 152 21 157 8.18e-14 65.9
MS.gene070099.t1 AT4G37010 28.467 137 98 0 16 152 25 161 8.46e-14 65.9
MS.gene070099.t1 AT3G07490 31.579 133 89 1 23 153 6 138 8.87e-14 65.1
MS.gene070099.t1 AT2G43290 32.847 137 85 3 23 152 66 202 1.43e-13 65.9
MS.gene070099.t1 AT4G14640 32.039 103 70 0 16 118 7 109 1.50e-13 63.9
MS.gene070099.t1 AT3G43810 30.189 106 74 0 47 152 1 106 5.00e-13 62.4
MS.gene070099.t1 AT3G43810 30.189 106 74 0 47 152 1 106 5.00e-13 62.4
MS.gene070099.t1 AT3G25600 27.778 144 102 1 15 156 5 148 5.09e-13 63.5
MS.gene070099.t1 AT3G10190 27.465 142 100 3 13 152 61 201 5.17e-13 64.3
MS.gene070099.t1 AT2G41410 34.694 147 80 7 23 159 71 211 5.64e-13 64.3
MS.gene070099.t1 AT3G22930 33.663 101 67 0 16 116 29 129 7.19e-13 62.4
MS.gene070099.t1 AT2G15680 27.273 132 90 2 23 152 51 178 1.12e-12 63.2
MS.gene070099.t1 AT2G41100 26.144 153 97 2 16 152 6 158 1.90e-12 63.9
MS.gene070099.t1 AT2G41100 25.595 168 105 3 5 152 81 248 7.59e-11 59.3
MS.gene070099.t1 AT2G41100 26.144 153 97 2 16 152 6 158 1.90e-12 63.9
MS.gene070099.t1 AT2G41100 25.595 168 105 3 5 152 81 248 7.59e-11 59.3
MS.gene070099.t1 AT1G18530 28.369 141 99 1 18 156 3 143 2.72e-12 61.6
MS.gene070099.t1 AT4G23650 28.289 152 102 3 1 152 367 511 3.93e-12 63.5
MS.gene070099.t1 AT1G76640 28.387 155 105 3 5 156 7 158 6.37e-12 60.5
MS.gene070099.t1 AT2G41860 27.451 153 105 2 6 158 348 494 5.40e-11 60.1
MS.gene070099.t1 AT2G41860 27.451 153 105 2 6 158 348 494 5.40e-11 60.1
MS.gene070099.t1 AT2G41100 25.595 168 105 3 5 152 46 213 6.29e-11 59.7
MS.gene070099.t1 AT2G41100 25.595 168 105 3 5 152 46 213 6.29e-11 59.7
MS.gene070099.t1 AT2G41100 25.595 168 105 3 5 152 46 213 6.29e-11 59.7
MS.gene070099.t1 AT5G19360 26.866 134 96 2 19 152 370 501 8.14e-11 59.7

Find 36 sgRNAs with CRISPR-Local

Find 39 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATTGTTGCTTCTCTTGATA+TGG 0.230821 5.4:-17885543 MS.gene070099:CDS
CATGAGGTGATGGGAAATTT+TGG 0.269755 5.4:-17885596 MS.gene070099:CDS
TTCTCATCTCTCTGTTGAAA+TGG 0.358991 5.4:-17885924 MS.gene070099:CDS
AAGTGCAGCGTTTGAAGCTT+TGG 0.363108 5.4:-17885657 MS.gene070099:CDS
AGGAGGAATCATGGGGTCAC+TGG 0.382463 5.4:-17885816 MS.gene070099:CDS
CAAGTTTCAATTGATGCAAA+TGG 0.385739 5.4:-17885957 None:intergenic
AGAGATGAACACAAAGGGTT+TGG 0.407031 5.4:-17885705 MS.gene070099:CDS
CAATCAAGTTCTGTCACTAA+TGG 0.407587 5.4:-17885894 MS.gene070099:CDS
AACACAAAGGGTTTGGAGAC+TGG 0.407732 5.4:-17885698 MS.gene070099:CDS
TTTGAAGCTTTGGATGAAGA+TGG 0.441935 5.4:-17885647 MS.gene070099:CDS
GGAGGAATCATGGGGTCACT+GGG 0.469532 5.4:-17885815 MS.gene070099:CDS
GGCAGAGCTAGGAGGAATCA+TGG 0.479002 5.4:-17885825 MS.gene070099:CDS
CAAGGTGACAAGAACAAAGA+TGG 0.479082 5.4:-17885752 MS.gene070099:CDS
TTCTTAGAGATGAACACAAA+GGG 0.500411 5.4:-17885710 MS.gene070099:CDS
GTTGCTTCTCTTGATATGGA+TGG 0.502637 5.4:-17885539 MS.gene070099:CDS
GCAGAGCTAGGAGGAATCAT+GGG 0.507839 5.4:-17885824 MS.gene070099:CDS
TTGAAGCTTTGGATGAAGAT+GGG 0.510505 5.4:-17885646 MS.gene070099:CDS
GGATGATTTCCGACTCATAG+TGG 0.513554 5.4:-17885498 MS.gene070099:CDS
ATGTAATATATCTTAAACTA+GGG 0.516431 5.4:+17885472 None:intergenic
TATGTAATATATCTTAAACT+AGG 0.524689 5.4:+17885471 None:intergenic
TGGTGATGGAGCTGTGAGCT+TGG 0.541436 5.4:-17885519 MS.gene070099:CDS
TGCATTAACGAGGCAGAGCT+AGG 0.543690 5.4:-17885836 MS.gene070099:CDS
GGGAGAGCTACATGAGGTGA+TGG 0.545118 5.4:-17885606 MS.gene070099:CDS
TCTCTTGATATGGATGGTGA+TGG 0.556032 5.4:-17885533 MS.gene070099:CDS
GGAGAGCTACATGAGGTGAT+GGG 0.583288 5.4:-17885605 MS.gene070099:CDS
AAGGTGACAAGAACAAAGAT+GGG 0.608897 5.4:-17885751 MS.gene070099:CDS
GAAGCATTCAAAGCTTTCGA+CGG 0.612389 5.4:-17885872 MS.gene070099:CDS
GCTTTCGACGGAGACAACGA+TGG 0.616688 5.4:-17885860 MS.gene070099:CDS
CTAGGGATTCCACTATGAGT+CGG 0.630184 5.4:+17885489 None:intergenic
CAACGATGGGTGCATTAACG+AGG 0.644925 5.4:-17885846 MS.gene070099:CDS
GTTCTTAGAGATGAACACAA+AGG 0.647298 5.4:-17885711 MS.gene070099:CDS
CTTTCGACGGAGACAACGAT+GGG 0.649619 5.4:-17885859 MS.gene070099:CDS
GTGAGAGCAATGATACAACA+AGG 0.679261 5.4:-17885770 MS.gene070099:CDS
GAGGAATCATGGGGTCACTG+GGG 0.686134 5.4:-17885814 MS.gene070099:CDS
ATTAACGAGGCAGAGCTAGG+AGG 0.697261 5.4:-17885833 MS.gene070099:CDS
CAGAGCTAGGAGGAATCATG+GGG 0.707619 5.4:-17885823 MS.gene070099:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
TTCTTAGAGATGAACACAAA+GGG - chr5.4:17885718-17885737 MS.gene070099:CDS 30.0%
! TATTGTTGCTTCTCTTGATA+TGG - chr5.4:17885885-17885904 MS.gene070099:CDS 30.0%
AAGGTGACAAGAACAAAGAT+GGG - chr5.4:17885677-17885696 MS.gene070099:CDS 35.0%
CAATCAAGTTCTGTCACTAA+TGG - chr5.4:17885534-17885553 MS.gene070099:CDS 35.0%
GTTCTTAGAGATGAACACAA+AGG - chr5.4:17885717-17885736 MS.gene070099:CDS 35.0%
TTCTCATCTCTCTGTTGAAA+TGG - chr5.4:17885504-17885523 MS.gene070099:CDS 35.0%
!! TTGAAGCTTTGGATGAAGAT+GGG - chr5.4:17885782-17885801 MS.gene070099:CDS 35.0%
!! TTTGAAGCTTTGGATGAAGA+TGG - chr5.4:17885781-17885800 MS.gene070099:CDS 35.0%
!!! ATGGGAATGAGATTTTGACT+GGG - chr5.4:17885800-17885819 MS.gene070099:CDS 35.0%
AGAGATGAACACAAAGGGTT+TGG - chr5.4:17885723-17885742 MS.gene070099:CDS 40.0%
CAAGGTGACAAGAACAAAGA+TGG - chr5.4:17885676-17885695 MS.gene070099:CDS 40.0%
GTGAGAGCAATGATACAACA+AGG - chr5.4:17885658-17885677 MS.gene070099:CDS 40.0%
GTTGCTTCTCTTGATATGGA+TGG - chr5.4:17885889-17885908 MS.gene070099:CDS 40.0%
TCTCTTGATATGGATGGTGA+TGG - chr5.4:17885895-17885914 MS.gene070099:CDS 40.0%
! GAAGCATTCAAAGCTTTCGA+CGG - chr5.4:17885556-17885575 MS.gene070099:CDS 40.0%
!! GATGGGAATGAGATTTTGAC+TGG - chr5.4:17885799-17885818 MS.gene070099:CDS 40.0%
!! GTGATGGGAAATTTTGGTCT+TGG - chr5.4:17885838-17885857 MS.gene070099:CDS 40.0%
!!! CATGAGGTGATGGGAAATTT+TGG - chr5.4:17885832-17885851 MS.gene070099:CDS 40.0%
!!! TGGGAATGAGATTTTGACTG+GGG - chr5.4:17885801-17885820 MS.gene070099:CDS 40.0%
AACACAAAGGGTTTGGAGAC+TGG - chr5.4:17885730-17885749 MS.gene070099:CDS 45.0%
CTAGGGATTCCACTATGAGT+CGG + chr5.4:17885942-17885961 None:intergenic 45.0%
GGATGATTTCCGACTCATAG+TGG - chr5.4:17885930-17885949 MS.gene070099:CDS 45.0%
! AAGTGCAGCGTTTGAAGCTT+TGG - chr5.4:17885771-17885790 MS.gene070099:CDS 45.0%
!!! GGGAATGAGATTTTGACTGG+GGG - chr5.4:17885802-17885821 MS.gene070099:CDS 45.0%
ATTAACGAGGCAGAGCTAGG+AGG - chr5.4:17885595-17885614 MS.gene070099:CDS 50.0%
CAACGATGGGTGCATTAACG+AGG - chr5.4:17885582-17885601 MS.gene070099:CDS 50.0%
CAGAGCTAGGAGGAATCATG+GGG - chr5.4:17885605-17885624 MS.gene070099:CDS 50.0%
CTTTCGACGGAGACAACGAT+GGG - chr5.4:17885569-17885588 MS.gene070099:CDS 50.0%
GCAGAGCTAGGAGGAATCAT+GGG - chr5.4:17885604-17885623 MS.gene070099:CDS 50.0%
GGAGAGCTACATGAGGTGAT+GGG - chr5.4:17885823-17885842 MS.gene070099:CDS 50.0%
TGCATTAACGAGGCAGAGCT+AGG - chr5.4:17885592-17885611 MS.gene070099:CDS 50.0%
AGGAGGAATCATGGGGTCAC+TGG - chr5.4:17885612-17885631 MS.gene070099:CDS 55.0%
GAGGAATCATGGGGTCACTG+GGG - chr5.4:17885614-17885633 MS.gene070099:CDS 55.0%
GCTTTCGACGGAGACAACGA+TGG - chr5.4:17885568-17885587 MS.gene070099:CDS 55.0%
GGAGGAATCATGGGGTCACT+GGG - chr5.4:17885613-17885632 MS.gene070099:CDS 55.0%
GGCAGAGCTAGGAGGAATCA+TGG - chr5.4:17885603-17885622 MS.gene070099:CDS 55.0%
GGGAGAGCTACATGAGGTGA+TGG - chr5.4:17885822-17885841 MS.gene070099:CDS 55.0%
!! TGGTGATGGAGCTGTGAGCT+TGG - chr5.4:17885909-17885928 MS.gene070099:CDS 55.0%
GACTGGGGGAGAGCTACATG+AGG - chr5.4:17885816-17885835 MS.gene070099:CDS 60.0%


Chromosome Type Strat End Strand Name
chr5.4 gene 17885484 17885966 17885484 ID=MS.gene070099
chr5.4 mRNA 17885484 17885966 17885484 ID=MS.gene070099.t1;Parent=MS.gene070099
chr5.4 exon 17885484 17885966 17885484 ID=MS.gene070099.t1.exon1;Parent=MS.gene070099.t1
chr5.4 CDS 17885484 17885966 17885484 ID=cds.MS.gene070099.t1;Parent=MS.gene070099.t1
Gene Sequence

>MS.gene070099

ATGCAAATGGCTCAAGTGAGTTCTCATCTCTCTGTTGAAATGGAGACACTCAATCAAGTTCTGTCACTAATGGAAGCATTCAAAGCTTTCGACGGAGACAACGATGGGTGCATTAACGAGGCAGAGCTAGGAGGAATCATGGGGTCACTGGGGTACAAAGCTAGTGATCAAGAAGTGAGAGCAATGATACAACAAGGTGACAAGAACAAAGATGGGTTATTATGTATAAGTGAGTTCTTAGAGATGAACACAAAGGGTTTGGAGACTGGAAATCTTGCAAATGTTCTAAGTGCAGCGTTTGAAGCTTTGGATGAAGATGGGAATGAGATTTTGACTGGGGGAGAGCTACATGAGGTGATGGGAAATTTTGGTCTTGGTTTGTCTTTAGAGAAATGTGAGAATATTGTTGCTTCTCTTGATATGGATGGTGATGGAGCTGTGAGCTTGGATGATTTCCGACTCATAGTGGAATCCCTAGTTTAA

Protein sequence

>MS.gene070099.t1

MQMAQVSSHLSVEMETLNQVLSLMEAFKAFDGDNDGCINEAELGGIMGSLGYKASDQEVRAMIQQGDKNKDGLLCISEFLEMNTKGLETGNLANVLSAAFEALDEDGNEILTGGELHEVMGNFGLGLSLEKCENIVASLDMDGDGAVSLDDFRLIVESLV