AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene053216


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene053216.t1 MTR_4g007030 100.000 259 0 0 1 259 1 259 0.0 524
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene053216.t1 AT3G58730 79.389 262 50 2 1 259 1 261 1.47e-152 426

Find 59 sgRNAs with CRISPR-Local

Find 63 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTTAGGTTGAAGAAGATTC+AGG 0.251278 4.2:+92567991 MS.gene053216:CDS
GAATACTATTAGTTACATTA+AGG 0.278798 4.2:+92567937 MS.gene053216:CDS
TTGAGAGAGAGGATTTCTTT+AGG 0.309365 4.2:+92567975 MS.gene053216:CDS
GTGTCGTGTTGCTTATATTA+AGG 0.324047 4.2:+92567793 MS.gene053216:CDS
AACTTGTTGGCTGGTACAAC+TGG 0.356161 4.2:+92568118 MS.gene053216:CDS
CGAAACCTCTCACAACTTGT+TGG 0.358369 4.2:+92568105 MS.gene053216:CDS
GTTGGATGAGCTTGAGAGAG+AGG 0.384353 4.2:+92567964 MS.gene053216:CDS
GACAATGCTTGGAGTGGTTA+AGG 0.388176 4.2:+92567427 MS.gene053216:CDS
TGTTTCAACAAAGGAATCAA+TGG 0.435503 4.2:+92567541 MS.gene053216:CDS
AATACTATTAGTTACATTAA+GGG 0.450457 4.2:+92567938 MS.gene053216:CDS
TAGTTACATTAAGGGTGAGT+TGG 0.451344 4.2:+92567946 MS.gene053216:CDS
AAAGGAAGATGGTGATTGCT+AGG 0.464882 4.2:+92568035 MS.gene053216:CDS
TGGAGTGGTTAAGGCACGTT+TGG 0.467712 4.2:+92567436 MS.gene053216:CDS
GTTTCAACAAAGGAATCAAT+GGG 0.471657 4.2:+92567542 MS.gene053216:CDS
ACCTCTCACAACTTGTTGGC+TGG 0.473238 4.2:+92568109 MS.gene053216:CDS
TGCTTATATTAAGGCGATTG+AGG 0.474951 4.2:+92567802 MS.gene053216:CDS
AAAAGTTACAATTGCAGCAA+GGG 0.488148 4.2:+92568074 MS.gene053216:CDS
CTAATAGTATTCTCCAACCT+CGG 0.495607 4.2:-92567926 None:intergenic
GTGGTTAAGGCACGTTTGGT+TGG 0.501885 4.2:+92567440 MS.gene053216:CDS
TTTAGGTTGAAGAAGATTCA+GGG 0.502528 4.2:+92567992 MS.gene053216:CDS
TAATAGCATCATCAAGGGTA+AGG 0.507164 4.2:-92567859 None:intergenic
ATTGGCTAGAGGTGGTCAGC+AGG 0.515670 4.2:+92567763 MS.gene053216:CDS
TCAGCACTGTAATCAAACTT+TGG 0.538953 4.2:-92567710 None:intergenic
TAGTCTTAATAGCATCATCA+AGG 0.542669 4.2:-92567865 None:intergenic
GAAAAGTTACAATTGCAGCA+AGG 0.543936 4.2:+92568073 MS.gene053216:CDS
GAGGGAAATTGAAAGGAAGA+TGG 0.546430 4.2:+92568024 MS.gene053216:CDS
GAATGTTGTTAAGCCGAGGT+TGG 0.546577 4.2:+92567913 MS.gene053216:CDS
AGATTCAGGGTTACAAGAGG+AGG 0.551823 4.2:+92568005 MS.gene053216:CDS
ACAAGAGGAGGGAAATTGAA+AGG 0.552871 4.2:+92568017 MS.gene053216:CDS
GTCCCCACAGTGACAATGCT+TGG 0.555756 4.2:+92567416 MS.gene053216:CDS
ATCATCAAGGGTAAGGAAAG+AGG 0.556678 4.2:-92567852 None:intergenic
TCTCGTACTGAGAATGTGGC+AGG 0.566710 4.2:+92567677 MS.gene053216:CDS
ATGTTAAAGAAGCTTCACTC+AGG 0.568588 4.2:+92567648 MS.gene053216:CDS
CTATTAAGACTACTAACCGT+AGG 0.570720 4.2:+92567876 MS.gene053216:CDS
ACTCCAAGCATTGTCACTGT+GGG 0.583853 4.2:-92567419 None:intergenic
ACTGAGGCTAAGTATGTAGC+CGG 0.591330 4.2:+92567596 MS.gene053216:CDS
GCAATCACAACGTTTGAATG+TGG 0.591655 4.2:+92567394 MS.gene053216:CDS
TTCTTCATTTGCATTAACTG+AGG 0.593026 4.2:+92567580 MS.gene053216:CDS
CGTTTGGTTGGAGCAACAAG+AGG 0.602491 4.2:+92567452 MS.gene053216:CDS
TTGAGAATGTTGTTAAGCCG+AGG 0.609957 4.2:+92567909 MS.gene053216:CDS
AAGGAAGATGGTGATTGCTA+GGG 0.610095 4.2:+92568036 MS.gene053216:CDS
CACTCCAAGCATTGTCACTG+TGG 0.610571 4.2:-92567420 None:intergenic
AAGTTTGATTACAGTGCTGA+CGG 0.611337 4.2:+92567713 MS.gene053216:CDS
GATTCAGGGTTACAAGAGGA+GGG 0.611778 4.2:+92568006 MS.gene053216:CDS
AGAAGATTCAGGGTTACAAG+AGG 0.614471 4.2:+92568002 MS.gene053216:CDS
TCTCAAGAGCATTAACCCTA+CGG 0.615251 4.2:-92567892 None:intergenic
AAAGTTACAATTGCAGCAAG+GGG 0.616721 4.2:+92568075 MS.gene053216:CDS
ACCAGCCAACAAGTTGTGAG+AGG 0.622099 4.2:-92568110 None:intergenic
AGTCTTAATAGCATCATCAA+GGG 0.622324 4.2:-92567864 None:intergenic
GACGTGCTTGATGTTATCAC+CGG 0.624967 4.2:-92567615 None:intergenic
ACGTTCTCGTACTGAGAATG+TGG 0.627068 4.2:+92567673 MS.gene053216:CDS
CACAGTGACAATGCTTGGAG+TGG 0.627138 4.2:+92567421 MS.gene053216:CDS
TATTAAGACTACTAACCGTA+GGG 0.628796 4.2:+92567877 MS.gene053216:CDS
TGTTAAAGAAGCTTCACTCA+GGG 0.649586 4.2:+92567649 MS.gene053216:CDS
CTGACTGGATTGGCTAGAGG+TGG 0.655401 4.2:+92567755 MS.gene053216:CDS
TGATTACAGTGCTGACGGTG+AGG 0.656119 4.2:+92567718 MS.gene053216:CDS
GATCTGACTGGATTGGCTAG+AGG 0.672119 4.2:+92567752 MS.gene053216:CDS
CTCCAAGCATTGTCACTGTG+GGG 0.709998 4.2:-92567418 None:intergenic
TGGTACAACTGGTAAAGACG+AGG 0.720395 4.2:+92568129 MS.gene053216:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATACTATTAGTTACATTAA+GGG + chr4.2:92567938-92567957 MS.gene053216:CDS 15.0%
!! GAATACTATTAGTTACATTA+AGG + chr4.2:92567937-92567956 MS.gene053216:CDS 20.0%
! GAAAAAGATTGTTTCAACAA+AGG + chr4.2:92567532-92567551 MS.gene053216:CDS 25.0%
AAAAGTTACAATTGCAGCAA+GGG + chr4.2:92568074-92568093 MS.gene053216:CDS 30.0%
AGTCTTAATAGCATCATCAA+GGG - chr4.2:92567867-92567886 None:intergenic 30.0%
GTTTCAACAAAGGAATCAAT+GGG + chr4.2:92567542-92567561 MS.gene053216:CDS 30.0%
TATTAAGACTACTAACCGTA+GGG + chr4.2:92567877-92567896 MS.gene053216:CDS 30.0%
TGTTTCAACAAAGGAATCAA+TGG + chr4.2:92567541-92567560 MS.gene053216:CDS 30.0%
TTCTTCATTTGCATTAACTG+AGG + chr4.2:92567580-92567599 MS.gene053216:CDS 30.0%
! TAGTCTTAATAGCATCATCA+AGG - chr4.2:92567868-92567887 None:intergenic 30.0%
!! TTTAGGTTGAAGAAGATTCA+GGG + chr4.2:92567992-92568011 MS.gene053216:CDS 30.0%
AAAGTTACAATTGCAGCAAG+GGG + chr4.2:92568075-92568094 MS.gene053216:CDS 35.0%
ATGTTAAAGAAGCTTCACTC+AGG + chr4.2:92567648-92567667 MS.gene053216:CDS 35.0%
CTAATAGTATTCTCCAACCT+CGG - chr4.2:92567929-92567948 None:intergenic 35.0%
CTATTAAGACTACTAACCGT+AGG + chr4.2:92567876-92567895 MS.gene053216:CDS 35.0%
GAAAAATGATCTGACTGGAT+TGG + chr4.2:92567745-92567764 MS.gene053216:CDS 35.0%
GAAAAGTTACAATTGCAGCA+AGG + chr4.2:92568073-92568092 MS.gene053216:CDS 35.0%
TAATAGCATCATCAAGGGTA+AGG - chr4.2:92567862-92567881 None:intergenic 35.0%
TAGTTACATTAAGGGTGAGT+TGG + chr4.2:92567946-92567965 MS.gene053216:CDS 35.0%
TCAGCACTGTAATCAAACTT+TGG - chr4.2:92567713-92567732 None:intergenic 35.0%
TGCTTATATTAAGGCGATTG+AGG + chr4.2:92567802-92567821 MS.gene053216:CDS 35.0%
TGTTAAAGAAGCTTCACTCA+GGG + chr4.2:92567649-92567668 MS.gene053216:CDS 35.0%
TTGAGAGAGAGGATTTCTTT+AGG + chr4.2:92567975-92567994 MS.gene053216:CDS 35.0%
! AAGTTTGATTACAGTGCTGA+CGG + chr4.2:92567713-92567732 MS.gene053216:CDS 35.0%
!! CTTTAGGTTGAAGAAGATTC+AGG + chr4.2:92567991-92568010 MS.gene053216:CDS 35.0%
!! GTGTCGTGTTGCTTATATTA+AGG + chr4.2:92567793-92567812 MS.gene053216:CDS 35.0%
AAAGGAAGATGGTGATTGCT+AGG + chr4.2:92568035-92568054 MS.gene053216:CDS 40.0%
AAGGAAGATGGTGATTGCTA+GGG + chr4.2:92568036-92568055 MS.gene053216:CDS 40.0%
ACAAGAGGAGGGAAATTGAA+AGG + chr4.2:92568017-92568036 MS.gene053216:CDS 40.0%
AGAAGATTCAGGGTTACAAG+AGG + chr4.2:92568002-92568021 MS.gene053216:CDS 40.0%
ATCATCAAGGGTAAGGAAAG+AGG - chr4.2:92567855-92567874 None:intergenic 40.0%
GAGGGAAATTGAAAGGAAGA+TGG + chr4.2:92568024-92568043 MS.gene053216:CDS 40.0%
GCAATCACAACGTTTGAATG+TGG + chr4.2:92567394-92567413 MS.gene053216:CDS 40.0%
TTGAGAATGTTGTTAAGCCG+AGG + chr4.2:92567909-92567928 MS.gene053216:CDS 40.0%
!! TCCAGTCAGATCATTTTTCG+TGG - chr4.2:92567744-92567763 None:intergenic 40.0%
!! TCTCAAGAGCATTAACCCTA+CGG - chr4.2:92567895-92567914 None:intergenic 40.0%
ACGTTCTCGTACTGAGAATG+TGG + chr4.2:92567673-92567692 MS.gene053216:CDS 45.0%
ACTGAGGCTAAGTATGTAGC+CGG + chr4.2:92567596-92567615 MS.gene053216:CDS 45.0%
AGATTCAGGGTTACAAGAGG+AGG + chr4.2:92568005-92568024 MS.gene053216:CDS 45.0%
CGAAACCTCTCACAACTTGT+TGG + chr4.2:92568105-92568124 MS.gene053216:CDS 45.0%
GAATGTTGTTAAGCCGAGGT+TGG + chr4.2:92567913-92567932 MS.gene053216:CDS 45.0%
GACAATGCTTGGAGTGGTTA+AGG + chr4.2:92567427-92567446 MS.gene053216:CDS 45.0%
GACGTGCTTGATGTTATCAC+CGG - chr4.2:92567618-92567637 None:intergenic 45.0%
GATTCAGGGTTACAAGAGGA+GGG + chr4.2:92568006-92568025 MS.gene053216:CDS 45.0%
GCCACGAAAAATGATCTGAC+TGG + chr4.2:92567740-92567759 MS.gene053216:CDS 45.0%
TGGTACAACTGGTAAAGACG+AGG + chr4.2:92568129-92568148 MS.gene053216:CDS 45.0%
! ACTCCAAGCATTGTCACTGT+GGG - chr4.2:92567422-92567441 None:intergenic 45.0%
!! AACTTGTTGGCTGGTACAAC+TGG + chr4.2:92568118-92568137 MS.gene053216:CDS 45.0%
ACCAGCCAACAAGTTGTGAG+AGG - chr4.2:92568113-92568132 None:intergenic 50.0%
CACAGTGACAATGCTTGGAG+TGG + chr4.2:92567421-92567440 MS.gene053216:CDS 50.0%
CGTTTGGTTGGAGCAACAAG+AGG + chr4.2:92567452-92567471 MS.gene053216:CDS 50.0%
GTGGTTAAGGCACGTTTGGT+TGG + chr4.2:92567440-92567459 MS.gene053216:CDS 50.0%
GTTGGATGAGCTTGAGAGAG+AGG + chr4.2:92567964-92567983 MS.gene053216:CDS 50.0%
TCTCGTACTGAGAATGTGGC+AGG + chr4.2:92567677-92567696 MS.gene053216:CDS 50.0%
TGGAGTGGTTAAGGCACGTT+TGG + chr4.2:92567436-92567455 MS.gene053216:CDS 50.0%
! CACTCCAAGCATTGTCACTG+TGG - chr4.2:92567423-92567442 None:intergenic 50.0%
! CTCCAAGCATTGTCACTGTG+GGG - chr4.2:92567421-92567440 None:intergenic 50.0%
!! ACCTCTCACAACTTGTTGGC+TGG + chr4.2:92568109-92568128 MS.gene053216:CDS 50.0%
!! GATCTGACTGGATTGGCTAG+AGG + chr4.2:92567752-92567771 MS.gene053216:CDS 50.0%
!! TGATTACAGTGCTGACGGTG+AGG + chr4.2:92567718-92567737 MS.gene053216:CDS 50.0%
ATTGGCTAGAGGTGGTCAGC+AGG + chr4.2:92567763-92567782 MS.gene053216:CDS 55.0%
GTCCCCACAGTGACAATGCT+TGG + chr4.2:92567416-92567435 MS.gene053216:CDS 55.0%
!! CTGACTGGATTGGCTAGAGG+TGG + chr4.2:92567755-92567774 MS.gene053216:CDS 55.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 92567386 92568165 92567386 ID=MS.gene053216
chr4.2 mRNA 92567386 92568165 92567386 ID=MS.gene053216.t1;Parent=MS.gene053216
chr4.2 exon 92567386 92568165 92567386 ID=MS.gene053216.t1.exon1;Parent=MS.gene053216.t1
chr4.2 CDS 92567386 92568165 92567386 ID=cds.MS.gene053216.t1;Parent=MS.gene053216.t1
Gene Sequence

>MS.gene053216

ATGTCGGGGCAATCACAACGTTTGAATGTGGTCCCCACAGTGACAATGCTTGGAGTGGTTAAGGCACGTTTGGTTGGAGCAACAAGAGGACATGCTCTTCTCAAGAAGAAAAGTGATGCTTTAACTGTTCAGTTTCGTCAGATCTTGAAAAAGATTGTTTCAACAAAGGAATCAATGGGTGACATAATGAAAACTTCTTCATTTGCATTAACTGAGGCTAAGTATGTAGCCGGTGATAACATCAAGCACGTCGTGCTTGAGAATGTTAAAGAAGCTTCACTCAGGGTACGTTCTCGTACTGAGAATGTGGCAGGAGTGAAGCTTCCAAAGTTTGATTACAGTGCTGACGGTGAGGCCACGAAAAATGATCTGACTGGATTGGCTAGAGGTGGTCAGCAGGTGCAGCAGTGTCGTGTTGCTTATATTAAGGCGATTGAGGTTCTTGTTGAGCTTGCTTCGCTTCAGACCTCTTTCCTTACCCTTGATGATGCTATTAAGACTACTAACCGTAGGGTTAATGCTCTTGAGAATGTTGTTAAGCCGAGGTTGGAGAATACTATTAGTTACATTAAGGGTGAGTTGGATGAGCTTGAGAGAGAGGATTTCTTTAGGTTGAAGAAGATTCAGGGTTACAAGAGGAGGGAAATTGAAAGGAAGATGGTGATTGCTAGGGAACAACAAGCTATTGAAAAGTTACAATTGCAGCAAGGGGTTTCTTTCGAAACCTCTCACAACTTGTTGGCTGGTACAACTGGTAAAGACGAGGATATCATTTTCTAA

Protein sequence

>MS.gene053216.t1

MSGQSQRLNVVPTVTMLGVVKARLVGATRGHALLKKKSDALTVQFRQILKKIVSTKESMGDIMKTSSFALTEAKYVAGDNIKHVVLENVKEASLRVRSRTENVAGVKLPKFDYSADGEATKNDLTGLARGGQQVQQCRVAYIKAIEVLVELASLQTSFLTLDDAIKTTNRRVNALENVVKPRLENTISYIKGELDELEREDFFRLKKIQGYKRREIERKMVIAREQQAIEKLQLQQGVSFETSHNLLAGTTGKDEDIIF