Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene070205.t1 | KEH33791.1 | 92.1 | 254 | 16 | 1 | 107 | 356 | 27 | 280 | 8.50E-83 | 317.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene070205.t1 | Q6JN46 | 35.0 | 103 | 60 | 1 | 259 | 354 | 914 | 1016 | 1.7e-10 | 68.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene070205.t1 | A0A072UWJ3 | 92.1 | 254 | 16 | 1 | 107 | 356 | 27 | 280 | 6.1e-83 | 317.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049993 | MS.gene070205 | 0.808146 | 3.57E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 71 sgRNAs with CRISPR-Local
Find 82 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTGGATTTGCAGCAGGTTTC+TGG | -0.003578 | 3.2:-41579407 | MS.gene070205:CDS |
| ACAATTGCAGATCATGATTT+TGG | 0.106542 | 3.2:-41580141 | MS.gene070205:CDS |
| AGAGGAGCTCCGCATAATTT+TGG | 0.204687 | 3.2:+41579522 | None:intergenic |
| CTTTGTTTCTTATTAGAAAA+TGG | 0.268683 | 3.2:-41579371 | MS.gene070205:CDS |
| CTTGCTTTAAACAAACTTTC+AGG | 0.270380 | 3.2:-41579984 | MS.gene070205:CDS |
| GTTTGAATTTGTGATCCTAT+TGG | 0.278276 | 3.2:+41579573 | None:intergenic |
| TCAGCCTTATTTGTTTCATT+TGG | 0.293427 | 3.2:-41580009 | MS.gene070205:CDS |
| TTTAATGCATCAAGCTTCAT+TGG | 0.302889 | 3.2:-41579549 | MS.gene070205:CDS |
| TTTGAATTTGTGATCCTATT+GGG | 0.304698 | 3.2:+41579574 | None:intergenic |
| TTCATGCCTCCAATCACTTT+TGG | 0.306951 | 3.2:+41579717 | None:intergenic |
| CAAGATCTATGTTACTTTGA+TGG | 0.318777 | 3.2:-41579310 | MS.gene070205:CDS |
| TTATCTCACAATAATTTGAT+TGG | 0.328914 | 3.2:-41579747 | MS.gene070205:CDS |
| TTGGGATAGTCCCTTCAAAT+TGG | 0.334248 | 3.2:+41580045 | None:intergenic |
| ATCAAAGAATCAATGTATCT+TGG | 0.339893 | 3.2:-41579438 | MS.gene070205:CDS |
| AGGTCAATAGTTCTTCTTCT+TGG | 0.356656 | 3.2:+41579840 | None:intergenic |
| CTTTCAGCAAATAGCTTGTC+TGG | 0.358680 | 3.2:-41579819 | MS.gene070205:CDS |
| TGGATTTGCAGCAGGTTTCT+GGG | 0.365964 | 3.2:-41579406 | MS.gene070205:CDS |
| TGTCTATAACTTGACTGATA+TGG | 0.381266 | 3.2:-41579946 | MS.gene070205:CDS |
| CGGGATCAATTCCACATAGT+TGG | 0.382996 | 3.2:-41580247 | MS.gene070205:CDS |
| AGGTCCAAATGAAACAAATA+AGG | 0.385591 | 3.2:+41580005 | None:intergenic |
| CTTTGATGGTAAAGTTGAAC+AGG | 0.394974 | 3.2:-41579296 | MS.gene070205:CDS |
| TTGGATAACAATAATTTCAT+AGG | 0.401019 | 3.2:-41580329 | MS.gene070205:CDS |
| TAAGCCATTGGTAATATGTT+CGG | 0.403644 | 3.2:+41580299 | None:intergenic |
| TTTGATGGAACAATCCCAAT+AGG | 0.408333 | 3.2:-41579588 | MS.gene070205:CDS |
| AATGGAGGCATGCATGCTTT+CGG | 0.421454 | 3.2:-41579353 | MS.gene070205:CDS |
| CTTGTCTGGAGAAGTGCCTT+TGG | 0.428859 | 3.2:-41579805 | MS.gene070205:CDS |
| TCAAAGAATCAATGTATCTT+GGG | 0.429179 | 3.2:-41579437 | MS.gene070205:CDS |
| ACAATTGAGTTGTTTACAAA+AGG | 0.429802 | 3.2:-41579888 | MS.gene070205:CDS |
| TTGTGATAAGTTCATTGGTA+TGG | 0.438242 | 3.2:+41580097 | None:intergenic |
| TTGTCTTACAACTCATTCTC+GGG | 0.439306 | 3.2:-41580266 | MS.gene070205:CDS |
| ATGGGAGTTGGATTTGCAGC+AGG | 0.443030 | 3.2:-41579414 | MS.gene070205:CDS |
| TTTGTCTTACAACTCATTCT+CGG | 0.450815 | 3.2:-41580267 | MS.gene070205:CDS |
| ATGTATCTTGGGATGGGAGT+TGG | 0.465620 | 3.2:-41579426 | MS.gene070205:CDS |
| GCTGATTGAATAATTGCGTT+GGG | 0.470289 | 3.2:+41580027 | None:intergenic |
| TCATCTTCATTCTGTGTAGA+TGG | 0.471699 | 3.2:+41579468 | None:intergenic |
| AAGCCATTGGTAATATGTTC+GGG | 0.473321 | 3.2:+41580300 | None:intergenic |
| TTCAAGTTCTTCCAACTATG+TGG | 0.478919 | 3.2:+41580236 | None:intergenic |
| GGATTGGTACAATTATTAAG+AGG | 0.479712 | 3.2:+41579504 | None:intergenic |
| GGCTGATTGAATAATTGCGT+TGG | 0.489338 | 3.2:+41580026 | None:intergenic |
| TTGAGAGCTAACCAATTTGA+AGG | 0.489346 | 3.2:-41580056 | MS.gene070205:CDS |
| CTGAAAGTTTGTTTAAAGCA+AGG | 0.499891 | 3.2:+41579985 | None:intergenic |
| AACTCAATTGTGGTACCTGC+TGG | 0.505072 | 3.2:+41579900 | None:intergenic |
| TCATTCTGTGTAGATGGCGT+TGG | 0.510792 | 3.2:+41579474 | None:intergenic |
| TTGTGGAGTAATAAGCTATC+CGG | 0.513583 | 3.2:-41580194 | MS.gene070205:CDS |
| TGGAGAAATTCCACCGAGCA+TGG | 0.522897 | 3.2:-41579655 | MS.gene070205:CDS |
| AGAATCAATGTATCTTGGGA+TGG | 0.523720 | 3.2:-41579433 | MS.gene070205:CDS |
| GGATTTGCAGCAGGTTTCTG+GGG | 0.524214 | 3.2:-41579405 | MS.gene070205:CDS |
| ATTTATATTTCATTAACTTG+TGG | 0.526189 | 3.2:-41580211 | MS.gene070205:CDS |
| AAATCAACTGCATAAGCCAT+TGG | 0.549986 | 3.2:+41580287 | None:intergenic |
| CTTTCGGTTTGTTGATAGAG+TGG | 0.550163 | 3.2:-41579337 | MS.gene070205:CDS |
| GATAACAATAATTTCATAGG+AGG | 0.551425 | 3.2:-41580326 | MS.gene070205:CDS |
| GAATCAATGTATCTTGGGAT+GGG | 0.556531 | 3.2:-41579432 | MS.gene070205:CDS |
| GATTTGCAGCAGGTTTCTGG+GGG | 0.570767 | 3.2:-41579404 | MS.gene070205:CDS |
| AAAATACCAAAAGTGATTGG+AGG | 0.571063 | 3.2:-41579723 | MS.gene070205:CDS |
| GAAGAGGAGGAATTACCAGC+AGG | 0.575112 | 3.2:-41579915 | MS.gene070205:CDS |
| CAGACAAGCTATTTGCTGAA+AGG | 0.576647 | 3.2:+41579820 | None:intergenic |
| TTTCGGTTTGTTGATAGAGT+GGG | 0.585715 | 3.2:-41579336 | MS.gene070205:CDS |
| TATGGTTACATTTGAAGAGG+AGG | 0.587819 | 3.2:-41579928 | MS.gene070205:CDS |
| TGATATGGTTACATTTGAAG+AGG | 0.594849 | 3.2:-41579931 | MS.gene070205:CDS |
| TGAGAGCTAACCAATTTGAA+GGG | 0.599073 | 3.2:-41580055 | MS.gene070205:CDS |
| GAGGTGCTGTATAACTTCAC+CGG | 0.599733 | 3.2:+41580175 | None:intergenic |
| GGCTCTCTTAAACTTTCTAG+AGG | 0.607477 | 3.2:-41579634 | MS.gene070205:CDS |
| ATTGGAAATCCAAAATTATG+CGG | 0.613640 | 3.2:-41579531 | MS.gene070205:CDS |
| TGTTTCTTATTAGAAAATGG+AGG | 0.620884 | 3.2:-41579368 | MS.gene070205:CDS |
| AAGTTTAAGAGAGCCATGCT+CGG | 0.628881 | 3.2:+41579642 | None:intergenic |
| GTCAAGTTATAGACACACTC+TGG | 0.643071 | 3.2:+41579954 | None:intergenic |
| TTATACAGCACCTCTCTGAG+TGG | 0.661294 | 3.2:-41580166 | MS.gene070205:CDS |
| TTTAAGAGAGCCATGCTCGG+TGG | 0.664745 | 3.2:+41579645 | None:intergenic |
| ACTCCCGAACATATTACCAA+TGG | 0.666984 | 3.2:-41580303 | MS.gene070205:CDS |
| ACAAGTCGAAACAATTCCAA+AGG | 0.668877 | 3.2:+41579789 | None:intergenic |
| GCAATTGTTTCCACTCAGAG+AGG | 0.685150 | 3.2:+41580156 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATTTATATTTCATTAACTTG+TGG | - | chr3.2:41579401-41579420 | MS.gene070205:CDS | 15.0% |
| !! | TTATCTCACAATAATTTGAT+TGG | - | chr3.2:41579865-41579884 | MS.gene070205:CDS | 20.0% |
| !! | TTGGATAACAATAATTTCAT+AGG | - | chr3.2:41579283-41579302 | MS.gene070205:CDS | 20.0% |
| !!! | AATTTTTGTGATAAGTTCAT+TGG | + | chr3.2:41579523-41579542 | None:intergenic | 20.0% |
| !!! | CTCTCAAATAATAAGTTTTT+TGG | - | chr3.2:41579937-41579956 | MS.gene070205:CDS | 20.0% |
| !!! | CTTTGTTTCTTATTAGAAAA+TGG | - | chr3.2:41580241-41580260 | MS.gene070205:CDS | 20.0% |
| !!! | TTATCTTGCAATAATTTTGA+TGG | - | chr3.2:41580009-41580028 | MS.gene070205:CDS | 20.0% |
| ! | ATCAAAGAATCAATGTATCT+TGG | - | chr3.2:41580174-41580193 | MS.gene070205:CDS | 25.0% |
| ! | ATTGGAAATCCAAAATTATG+CGG | - | chr3.2:41580081-41580100 | MS.gene070205:CDS | 25.0% |
| ! | GATAACAATAATTTCATAGG+AGG | - | chr3.2:41579286-41579305 | MS.gene070205:CDS | 25.0% |
| ! | TCAAAGAATCAATGTATCTT+GGG | - | chr3.2:41580175-41580194 | MS.gene070205:CDS | 25.0% |
| ! | TGTTTCTTATTAGAAAATGG+AGG | - | chr3.2:41580244-41580263 | MS.gene070205:CDS | 25.0% |
| ! | TTTGAATTTGTGATCCTATT+GGG | + | chr3.2:41580041-41580060 | None:intergenic | 25.0% |
| !! | ACAATTGAGTTGTTTACAAA+AGG | - | chr3.2:41579724-41579743 | MS.gene070205:CDS | 25.0% |
| !! | TTGGGAGAAAATAAATTTTC+AGG | - | chr3.2:41579490-41579509 | MS.gene070205:CDS | 25.0% |
| !! | TTTTGTAAACAACTCAATTG+TGG | + | chr3.2:41579725-41579744 | None:intergenic | 25.0% |
| AAAATACCAAAAGTGATTGG+AGG | - | chr3.2:41579889-41579908 | MS.gene070205:CDS | 30.0% | |
| AGGTCCAAATGAAACAAATA+AGG | + | chr3.2:41579610-41579629 | None:intergenic | 30.0% | |
| GGAAAAATACCAAAAGTGAT+TGG | - | chr3.2:41579886-41579905 | MS.gene070205:CDS | 30.0% | |
| GTTTGAATTTGTGATCCTAT+TGG | + | chr3.2:41580042-41580061 | None:intergenic | 30.0% | |
| TGATATGGTTACATTTGAAG+AGG | - | chr3.2:41579681-41579700 | MS.gene070205:CDS | 30.0% | |
| TGTCTATAACTTGACTGATA+TGG | - | chr3.2:41579666-41579685 | MS.gene070205:CDS | 30.0% | |
| TTGTGATAAGTTCATTGGTA+TGG | + | chr3.2:41579518-41579537 | None:intergenic | 30.0% | |
| TTTAATGCATCAAGCTTCAT+TGG | - | chr3.2:41580063-41580082 | MS.gene070205:CDS | 30.0% | |
| TTTGTCTTACAACTCATTCT+CGG | - | chr3.2:41579345-41579364 | MS.gene070205:CDS | 30.0% | |
| ! | ACAATTGCAGATCATGATTT+TGG | - | chr3.2:41579471-41579490 | MS.gene070205:CDS | 30.0% |
| ! | CAATTGCAGATCATGATTTT+GGG | - | chr3.2:41579472-41579491 | MS.gene070205:CDS | 30.0% |
| ! | CTGAAAGTTTGTTTAAAGCA+AGG | + | chr3.2:41579630-41579649 | None:intergenic | 30.0% |
| ! | CTTGCTTTAAACAAACTTTC+AGG | - | chr3.2:41579628-41579647 | MS.gene070205:CDS | 30.0% |
| ! | GGATTGGTACAATTATTAAG+AGG | + | chr3.2:41580111-41580130 | None:intergenic | 30.0% |
| ! | TAAGCCATTGGTAATATGTT+CGG | + | chr3.2:41579316-41579335 | None:intergenic | 30.0% |
| !! | CAAGATCTATGTTACTTTGA+TGG | - | chr3.2:41580302-41580321 | MS.gene070205:CDS | 30.0% |
| !! | TCAGCCTTATTTGTTTCATT+TGG | - | chr3.2:41579603-41579622 | MS.gene070205:CDS | 30.0% |
| AAATCAACTGCATAAGCCAT+TGG | + | chr3.2:41579328-41579347 | None:intergenic | 35.0% | |
| ACAAGTCGAAACAATTCCAA+AGG | + | chr3.2:41579826-41579845 | None:intergenic | 35.0% | |
| AGAATCAATGTATCTTGGGA+TGG | - | chr3.2:41580179-41580198 | MS.gene070205:CDS | 35.0% | |
| AGGTCAATAGTTCTTCTTCT+TGG | + | chr3.2:41579775-41579794 | None:intergenic | 35.0% | |
| GAATCAATGTATCTTGGGAT+GGG | - | chr3.2:41580180-41580199 | MS.gene070205:CDS | 35.0% | |
| GCTGATTGAATAATTGCGTT+GGG | + | chr3.2:41579588-41579607 | None:intergenic | 35.0% | |
| TATGGTTACATTTGAAGAGG+AGG | - | chr3.2:41579684-41579703 | MS.gene070205:CDS | 35.0% | |
| TCATCTTCATTCTGTGTAGA+TGG | + | chr3.2:41580147-41580166 | None:intergenic | 35.0% | |
| TGAGAGCTAACCAATTTGAA+GGG | - | chr3.2:41579557-41579576 | MS.gene070205:CDS | 35.0% | |
| TTGAGAGCTAACCAATTTGA+AGG | - | chr3.2:41579556-41579575 | MS.gene070205:CDS | 35.0% | |
| TTGTCTTACAACTCATTCTC+GGG | - | chr3.2:41579346-41579365 | MS.gene070205:CDS | 35.0% | |
| TTGTGGAGTAATAAGCTATC+CGG | - | chr3.2:41579418-41579437 | MS.gene070205:CDS | 35.0% | |
| TTTGATGGAACAATCCCAAT+AGG | - | chr3.2:41580024-41580043 | MS.gene070205:CDS | 35.0% | |
| ! | AAGCCATTGGTAATATGTTC+GGG | + | chr3.2:41579315-41579334 | None:intergenic | 35.0% |
| ! | CTTTGATGGTAAAGTTGAAC+AGG | - | chr3.2:41580316-41580335 | MS.gene070205:CDS | 35.0% |
| ! | GATTGGAGGCATGAAAAATA+TGG | - | chr3.2:41579903-41579922 | MS.gene070205:CDS | 35.0% |
| ! | TTCAAGTTCTTCCAACTATG+TGG | + | chr3.2:41579379-41579398 | None:intergenic | 35.0% |
| !! | TTTCGGTTTGTTGATAGAGT+GGG | - | chr3.2:41580276-41580295 | MS.gene070205:CDS | 35.0% |
| AAGTTTAAGAGAGCCATGCT+CGG | + | chr3.2:41579973-41579992 | None:intergenic | 40.0% | |
| ACTCCCGAACATATTACCAA+TGG | - | chr3.2:41579309-41579328 | MS.gene070205:CDS | 40.0% | |
| GGCTCTCTTAAACTTTCTAG+AGG | - | chr3.2:41579978-41579997 | MS.gene070205:CDS | 40.0% | |
| GGCTGATTGAATAATTGCGT+TGG | + | chr3.2:41579589-41579608 | None:intergenic | 40.0% | |
| GTCAAGTTATAGACACACTC+TGG | + | chr3.2:41579661-41579680 | None:intergenic | 40.0% | |
| TTGGGATAGTCCCTTCAAAT+TGG | + | chr3.2:41579570-41579589 | None:intergenic | 40.0% | |
| ! | CAGACAAGCTATTTGCTGAA+AGG | + | chr3.2:41579795-41579814 | None:intergenic | 40.0% |
| ! | CTTTCAGCAAATAGCTTGTC+TGG | - | chr3.2:41579793-41579812 | MS.gene070205:CDS | 40.0% |
| ! | TTCATGCCTCCAATCACTTT+TGG | + | chr3.2:41579898-41579917 | None:intergenic | 40.0% |
| !! | CTTTCGGTTTGTTGATAGAG+TGG | - | chr3.2:41580275-41580294 | MS.gene070205:CDS | 40.0% |
| AACTCAATTGTGGTACCTGC+TGG | + | chr3.2:41579715-41579734 | None:intergenic | 45.0% | |
| ATGTATCTTGGGATGGGAGT+TGG | - | chr3.2:41580186-41580205 | MS.gene070205:CDS | 45.0% | |
| CGGGATCAATTCCACATAGT+TGG | - | chr3.2:41579365-41579384 | MS.gene070205:CDS | 45.0% | |
| GCAATTGTTTCCACTCAGAG+AGG | + | chr3.2:41579459-41579478 | None:intergenic | 45.0% | |
| TCATTCTGTGTAGATGGCGT+TGG | + | chr3.2:41580141-41580160 | None:intergenic | 45.0% | |
| TGGATTTGCAGCAGGTTTCT+GGG | - | chr3.2:41580206-41580225 | MS.gene070205:CDS | 45.0% | |
| TTATACAGCACCTCTCTGAG+TGG | - | chr3.2:41579446-41579465 | MS.gene070205:CDS | 45.0% | |
| TTGGATTTGCAGCAGGTTTC+TGG | - | chr3.2:41580205-41580224 | MS.gene070205:CDS | 45.0% | |
| ! | AATGGAGGCATGCATGCTTT+CGG | - | chr3.2:41580259-41580278 | MS.gene070205:CDS | 45.0% |
| ! | AGAGGAGCTCCGCATAATTT+TGG | + | chr3.2:41580093-41580112 | None:intergenic | 45.0% |
| !! | GAGGTGCTGTATAACTTCAC+CGG | + | chr3.2:41579440-41579459 | None:intergenic | 45.0% |
| !! | GTAGATGGCGTTGGAGTTTT+AGG | + | chr3.2:41580132-41580151 | None:intergenic | 45.0% |
| !!! | TGGCGTTGGAGTTTTAGGAT+TGG | + | chr3.2:41580127-41580146 | None:intergenic | 45.0% |
| ATGGGAGTTGGATTTGCAGC+AGG | - | chr3.2:41580198-41580217 | MS.gene070205:CDS | 50.0% | |
| GAAGAGGAGGAATTACCAGC+AGG | - | chr3.2:41579697-41579716 | MS.gene070205:CDS | 50.0% | |
| GGATTTGCAGCAGGTTTCTG+GGG | - | chr3.2:41580207-41580226 | MS.gene070205:CDS | 50.0% | |
| TGGAGAAATTCCACCGAGCA+TGG | - | chr3.2:41579957-41579976 | MS.gene070205:CDS | 50.0% | |
| TTTAAGAGAGCCATGCTCGG+TGG | + | chr3.2:41579970-41579989 | None:intergenic | 50.0% | |
| ! | GATTTGCAGCAGGTTTCTGG+GGG | - | chr3.2:41580208-41580227 | MS.gene070205:CDS | 50.0% |
| !! | CTTGTCTGGAGAAGTGCCTT+TGG | - | chr3.2:41579807-41579826 | MS.gene070205:CDS | 50.0% |
| ! | GCAGGTTTCTGGGGGATTTG+TGG | - | chr3.2:41580216-41580235 | MS.gene070205:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.2 | gene | 41579281 | 41580353 | 41579281 | ID=MS.gene070205 |
| chr3.2 | mRNA | 41579281 | 41580353 | 41579281 | ID=MS.gene070205.t1;Parent=MS.gene070205 |
| chr3.2 | exon | 41579281 | 41580353 | 41579281 | ID=MS.gene070205.t1.exon1;Parent=MS.gene070205.t1 |
| chr3.2 | CDS | 41579281 | 41580353 | 41579281 | ID=cds.MS.gene070205.t1;Parent=MS.gene070205.t1 |
| Gene Sequence |
| Protein sequence |
