Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene070218.t1 | XP_003605646.1 | 100 | 211 | 0 | 0 | 1 | 211 | 62 | 272 | 1.70E-110 | 408.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene070218.t1 | Q9M1S3 | 40.6 | 101 | 42 | 4 | 1 | 92 | 63 | 154 | 4.3e-06 | 53.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene070218.t1 | G7JRC4 | 100.0 | 211 | 0 | 0 | 1 | 211 | 62 | 272 | 1.2e-110 | 408.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene052129 | MS.gene070218 | 0.817877 | 2.62E-52 | -1.69E-46 |
| MS.gene055251 | MS.gene070218 | 0.819757 | 9.82E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene070218.t1 | MTR_4g035310 | 100.000 | 211 | 0 | 0 | 1 | 211 | 62 | 272 | 2.05e-149 | 416 |
| MS.gene070218.t1 | MTR_4g035310 | 100.000 | 198 | 0 | 0 | 1 | 198 | 62 | 259 | 9.44e-139 | 389 |
| MS.gene070218.t1 | MTR_8g024520 | 38.119 | 202 | 90 | 11 | 1 | 197 | 71 | 242 | 2.29e-21 | 89.4 |
| MS.gene070218.t1 | MTR_8g024520 | 38.119 | 202 | 90 | 11 | 1 | 197 | 71 | 242 | 2.36e-21 | 89.4 |
| MS.gene070218.t1 | MTR_3g116130 | 41.212 | 165 | 76 | 5 | 1 | 161 | 75 | 222 | 6.66e-21 | 88.2 |
| MS.gene070218.t1 | MTR_3g116130 | 41.212 | 165 | 76 | 5 | 1 | 161 | 75 | 222 | 2.23e-20 | 88.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene070218.t1 | AT1G33470 | 53.589 | 209 | 77 | 8 | 1 | 206 | 53 | 244 | 3.00e-59 | 186 |
| MS.gene070218.t1 | AT1G33470 | 54.455 | 202 | 71 | 9 | 1 | 198 | 53 | 237 | 1.85e-57 | 182 |
| MS.gene070218.t1 | AT1G33470 | 53.333 | 210 | 77 | 9 | 1 | 206 | 53 | 245 | 2.25e-57 | 182 |
| MS.gene070218.t1 | AT1G22910 | 58.400 | 125 | 40 | 5 | 1 | 119 | 59 | 177 | 3.00e-35 | 125 |
| MS.gene070218.t1 | AT1G22910 | 58.400 | 125 | 40 | 5 | 1 | 119 | 8 | 126 | 5.34e-35 | 123 |
| MS.gene070218.t1 | AT1G22910 | 58.400 | 125 | 40 | 5 | 1 | 119 | 59 | 177 | 5.77e-35 | 124 |
| MS.gene070218.t1 | AT1G22910 | 58.400 | 125 | 40 | 5 | 1 | 119 | 59 | 177 | 7.72e-35 | 126 |
| MS.gene070218.t1 | AT2G46780 | 39.456 | 147 | 49 | 6 | 1 | 124 | 68 | 197 | 3.62e-18 | 81.6 |
| MS.gene070218.t1 | AT2G46780 | 39.456 | 147 | 49 | 6 | 1 | 124 | 68 | 197 | 3.62e-18 | 81.6 |
| MS.gene070218.t1 | AT1G20880 | 44.706 | 170 | 65 | 11 | 1 | 159 | 70 | 221 | 2.85e-17 | 78.6 |
| MS.gene070218.t1 | AT1G20880 | 44.706 | 170 | 65 | 11 | 1 | 159 | 70 | 221 | 2.85e-17 | 78.6 |
| MS.gene070218.t1 | AT1G20880 | 44.706 | 170 | 65 | 11 | 1 | 159 | 70 | 221 | 2.85e-17 | 78.6 |
| MS.gene070218.t1 | AT1G76460 | 38.690 | 168 | 82 | 7 | 1 | 161 | 70 | 223 | 2.11e-16 | 76.3 |
| MS.gene070218.t1 | AT1G22330 | 49.231 | 65 | 30 | 2 | 1 | 64 | 63 | 125 | 2.37e-11 | 62.0 |
Find 87 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGGATACCTTATAATGTTTA+TGG | 0.144330 | 4.3:-65671083 | MS.gene070218:CDS |
| CAACTGCAACCTTGCTTCTT+TGG | 0.147666 | 4.3:-65672581 | MS.gene070218:CDS |
| AACTGCAACCTTGCTTCTTT+GGG | 0.227071 | 4.3:-65672580 | MS.gene070218:CDS |
| CCTTCGCCATATTGAAGATA+TGG | 0.241363 | 4.3:+65666880 | None:intergenic |
| ACAGCACAGTATCCTGTTTA+TGG | 0.260295 | 4.3:-65666958 | MS.gene070218:CDS |
| GAGGAGGAGGGAGGAACATT+AGG | 0.264611 | 4.3:-65671193 | MS.gene070218:CDS |
| GGTTCTTTCCAGACAGGATT+TGG | 0.274909 | 4.3:-65671164 | MS.gene070218:CDS |
| GTGTTGAAGGCTTAGATCTT+TGG | 0.295951 | 4.3:+65672554 | None:intergenic |
| GCTCTTCTACCATCAATTAT+AGG | 0.296553 | 4.3:+65672604 | None:intergenic |
| ACTACCGGCGGCTACACTTC+TGG | 0.318698 | 4.3:-65666844 | MS.gene070218:CDS |
| TCATACCCATAAACATTATA+AGG | 0.319240 | 4.3:+65671077 | None:intergenic |
| TACCGTAGGGTAACTGTAAT+CGG | 0.319248 | 4.3:+65669969 | None:intergenic |
| GTATTAATATTACCATGTTT+TGG | 0.340726 | 4.3:+65672532 | None:intergenic |
| TCTCCTGCTGCCTTGCCATC+AGG | 0.355688 | 4.3:-65666715 | MS.gene070218:intron |
| CATTATAAGGTATCCCTTGC+TGG | 0.367371 | 4.3:+65671090 | None:intergenic |
| CAGTATCCTGTTTATGGAAA+TGG | 0.374418 | 4.3:-65666952 | MS.gene070218:CDS |
| GGATACCTTATAATGTTTAT+GGG | 0.377409 | 4.3:-65671082 | MS.gene070218:intron |
| TACACTTCTGGCCAAGGTTA+TGG | 0.379037 | 4.3:-65666832 | MS.gene070218:CDS |
| TGGAAAATGCAGAGCTACTA+TGG | 0.382407 | 4.3:-65666997 | MS.gene070218:intron |
| AGGAGGAGGGAGGAACATTA+GGG | 0.383370 | 4.3:-65671192 | MS.gene070218:CDS |
| TACTACCCATATCTTCAATA+TGG | 0.386372 | 4.3:-65666886 | MS.gene070218:CDS |
| TGAAAATTTGTAGGAGGAGG+AGG | 0.391447 | 4.3:-65671206 | MS.gene070218:intron |
| AGTCTCTAACGATGAGGAAT+TGG | 0.395605 | 4.3:+65666062 | None:intergenic |
| CTTCAATATGGCGAAGGAAC+CGG | 0.396046 | 4.3:-65666874 | MS.gene070218:CDS |
| TATTCTCCAATTGCTTCAAC+TGG | 0.407811 | 4.3:-65666781 | MS.gene070218:CDS |
| GCTGGTCCATTTCCATAAAC+AGG | 0.416170 | 4.3:+65666946 | None:intergenic |
| GGAGAAGGAGCAAGAGAAAT+GGG | 0.426995 | 4.3:+65666733 | None:intergenic |
| AGGAGAAGGAGCAAGAGAAA+TGG | 0.429829 | 4.3:+65666732 | None:intergenic |
| TACCCACCAGTTGAAGCAAT+TGG | 0.437493 | 4.3:+65666775 | None:intergenic |
| GGTGGGTATGCTCAGCACTA+TGG | 0.438801 | 4.3:-65666760 | MS.gene070218:CDS |
| CCACTCCTCCAAATCCTGTC+TGG | 0.440497 | 4.3:+65671156 | None:intergenic |
| CTTCGCCATATTGAAGATAT+GGG | 0.453891 | 4.3:+65666881 | None:intergenic |
| AGGGAGGAACATTAGGGTAA+TGG | 0.461392 | 4.3:-65671186 | MS.gene070218:CDS |
| ACCGTAGGGTAACTGTAATC+GGG | 0.463847 | 4.3:+65669970 | None:intergenic |
| GGGAGGAACATTAGGGTAAT+GGG | 0.468258 | 4.3:-65671185 | MS.gene070218:CDS |
| GCCGCCGGTAGTTGCGCCGC+CGG | 0.471628 | 4.3:+65666855 | None:intergenic |
| CAGACAGGATTTGGAGGAGT+GGG | 0.480274 | 4.3:-65671155 | MS.gene070218:CDS |
| AAACCTGATGGCAAGGCAGC+AGG | 0.480985 | 4.3:+65666712 | None:intergenic |
| TCTCCAATTGCTTCAACTGG+TGG | 0.504755 | 4.3:-65666778 | MS.gene070218:CDS |
| CAAGCTCTCATAGCAGCATC+AGG | 0.508307 | 4.3:+65672640 | None:intergenic |
| GATGGCAAGGCAGCAGGAGA+AGG | 0.510975 | 4.3:+65666718 | None:intergenic |
| GTTTATGGAAATGGACCAGC+TGG | 0.512520 | 4.3:-65666943 | MS.gene070218:CDS |
| GATGCTGCTCCTATAATTGA+TGG | 0.515656 | 4.3:-65672613 | MS.gene070218:CDS |
| GGAACCGGCGGCGCAACTAC+CGG | 0.519759 | 4.3:-65666859 | MS.gene070218:CDS |
| GTAATGGGTTCTTTCCAGAC+AGG | 0.536127 | 4.3:-65671170 | MS.gene070218:CDS |
| GAAAATTTGTAGGAGGAGGA+GGG | 0.539063 | 4.3:-65671205 | MS.gene070218:intron |
| CCTTGCTGGATGGCATAATG+AGG | 0.539851 | 4.3:+65671104 | None:intergenic |
| CAAAGAAGCAAGGTTGCAGT+TGG | 0.540744 | 4.3:+65672582 | None:intergenic |
| ATATTGAAGATATGGGTAGT+AGG | 0.540954 | 4.3:+65666888 | None:intergenic |
| CTCCAATTGCTTCAACTGGT+GGG | 0.542942 | 4.3:-65666777 | MS.gene070218:CDS |
| CCAGTCATCATCCCTCCAGC+TGG | 0.546713 | 4.3:+65666928 | None:intergenic |
| GAATATTGAAACACCTGAGG+TGG | 0.547295 | 4.3:+65666799 | None:intergenic |
| TCTTTCCAGACAGGATTTGG+AGG | 0.549169 | 4.3:-65671161 | MS.gene070218:CDS |
| GCTACTATGGTATGTACGGT+GGG | 0.552444 | 4.3:-65666984 | MS.gene070218:CDS |
| TGCTGCTATGAGAGCTTGTG+TGG | 0.555924 | 4.3:-65672635 | MS.gene070218:CDS |
| CCAGCTGGAGGGATGATGAC+TGG | 0.557715 | 4.3:-65666928 | MS.gene070218:CDS |
| CAGAGCTACTATGGTATGTA+CGG | 0.561819 | 4.3:-65666988 | MS.gene070218:CDS |
| CCAGACAGGATTTGGAGGAG+TGG | 0.563630 | 4.3:-65671156 | MS.gene070218:CDS |
| TGGTGTTAACTACCCACCTC+AGG | 0.572192 | 4.3:-65666812 | MS.gene070218:CDS |
| CTCATTATGCCATCCAGCAA+GGG | 0.572637 | 4.3:-65671103 | MS.gene070218:CDS |
| GAAGTAGAAACCTGATGGCA+AGG | 0.575970 | 4.3:+65666705 | None:intergenic |
| CCTCATTATGCCATCCAGCA+AGG | 0.580886 | 4.3:-65671104 | MS.gene070218:CDS |
| TGAGGGAAGGTTGCAGCTGA+AGG | 0.584132 | 4.3:+65671122 | None:intergenic |
| CCATATCTTCAATATGGCGA+AGG | 0.596896 | 4.3:-65666880 | MS.gene070218:CDS |
| GGCGGCTACACTTCTGGCCA+AGG | 0.598379 | 4.3:-65666838 | MS.gene070218:CDS |
| ATAAGGTATCCCTTGCTGGA+TGG | 0.606510 | 4.3:+65671094 | None:intergenic |
| CCCCGATTACAGTTACCCTA+CGG | 0.615766 | 4.3:-65669971 | MS.gene070218:intron |
| CCGTAGGGTAACTGTAATCG+GGG | 0.616563 | 4.3:+65669971 | None:intergenic |
| CTGTGCCACAGGCGTGACAA+TGG | 0.617286 | 4.3:-65666096 | MS.gene070218:intron |
| TATGGAAATGGACCAGCTGG+AGG | 0.625601 | 4.3:-65666940 | MS.gene070218:CDS |
| GGTAGTTAACACCATAACCT+TGG | 0.625763 | 4.3:+65666821 | None:intergenic |
| TTTGGACACCCAAAGAAGCA+AGG | 0.627687 | 4.3:+65672572 | None:intergenic |
| CTTGCTGGATGGCATAATGA+GGG | 0.627859 | 4.3:+65671105 | None:intergenic |
| AATTTGTAGGAGGAGGAGGG+AGG | 0.630642 | 4.3:-65671202 | MS.gene070218:intron |
| ACCGGCGGCGCAACTACCGG+CGG | 0.633500 | 4.3:-65666856 | MS.gene070218:CDS |
| ATGGAAATGGACCAGCTGGA+GGG | 0.637061 | 4.3:-65666939 | MS.gene070218:CDS |
| TTGGCCAGAAGTGTAGCCGC+CGG | 0.638787 | 4.3:+65666840 | None:intergenic |
| AGCTACTATGGTATGTACGG+TGG | 0.639695 | 4.3:-65666985 | MS.gene070218:CDS |
| AAGCCTTCAACACCAAAACA+TGG | 0.645444 | 4.3:-65672544 | MS.gene070218:intron |
| AATATTGAAACACCTGAGGT+GGG | 0.650438 | 4.3:+65666800 | None:intergenic |
| CAATATGGCGAAGGAACCGG+CGG | 0.663093 | 4.3:-65666871 | MS.gene070218:CDS |
| CTGGATGGCATAATGAGGGA+AGG | 0.669727 | 4.3:+65671109 | None:intergenic |
| CTACTATGGTATGTACGGTG+GGG | 0.669988 | 4.3:-65666983 | MS.gene070218:CDS |
| GATATGAGTCTCTAACGATG+AGG | 0.693503 | 4.3:+65666056 | None:intergenic |
| GGAGAATATTGAAACACCTG+AGG | 0.699373 | 4.3:+65666796 | None:intergenic |
| CGTAGGGTAACTGTAATCGG+GGG | 0.726499 | 4.3:+65669972 | None:intergenic |
| GCTGGAGGGATGATGACTGG+TGG | 0.745650 | 4.3:-65666925 | MS.gene070218:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.3 | gene | 65666067 | 65672677 | 65666067 | ID=MS.gene070218 |
| chr4.3 | mRNA | 65666067 | 65672677 | 65666067 | ID=MS.gene070218.t1;Parent=MS.gene070218 |
| chr4.3 | exon | 65672545 | 65672677 | 65672545 | ID=MS.gene070218.t1.exon1;Parent=MS.gene070218.t1 |
| chr4.3 | CDS | 65672545 | 65672677 | 65672545 | ID=cds.MS.gene070218.t1;Parent=MS.gene070218.t1 |
| chr4.3 | exon | 65671083 | 65671215 | 65671083 | ID=MS.gene070218.t1.exon2;Parent=MS.gene070218.t1 |
| chr4.3 | CDS | 65671083 | 65671215 | 65671083 | ID=cds.MS.gene070218.t1;Parent=MS.gene070218.t1 |
| chr4.3 | exon | 65669972 | 65670008 | 65669972 | ID=MS.gene070218.t1.exon3;Parent=MS.gene070218.t1 |
| chr4.3 | CDS | 65669972 | 65670008 | 65669972 | ID=cds.MS.gene070218.t1;Parent=MS.gene070218.t1 |
| chr4.3 | exon | 65666716 | 65667007 | 65666716 | ID=MS.gene070218.t1.exon4;Parent=MS.gene070218.t1 |
| chr4.3 | CDS | 65666716 | 65667007 | 65666716 | ID=cds.MS.gene070218.t1;Parent=MS.gene070218.t1 |
| chr4.3 | exon | 65666067 | 65666107 | 65666067 | ID=MS.gene070218.t1.exon5;Parent=MS.gene070218.t1 |
| chr4.3 | CDS | 65666067 | 65666107 | 65666067 | ID=cds.MS.gene070218.t1;Parent=MS.gene070218.t1 |
| Gene Sequence |
| Protein sequence |
