Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene070514.t1 | TKY56988.1 | 32.2 | 211 | 108 | 7 | 3 | 207 | 234 | 415 | 3.30E-13 | 85.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene070514.t1 | A0A151U1F8 | 32.7 | 220 | 101 | 10 | 2 | 213 | 78 | 258 | 3.5e-12 | 81.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene057887 | MS.gene070514 | 0.837412 | 5.28E-57 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 29 sgRNAs with CRISPR-Local
Find 37 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCTATGAGCAAGTTCACTTT+TGG | 0.280909 | 5.4:+67583246 | None:intergenic |
| AAGGTAATGATTATTGATTC+AGG | 0.310716 | 5.4:+67583426 | MS.gene070514:CDS |
| GAATGGTCTTGATCTGTTAA+AGG | 0.324061 | 5.4:-67583303 | None:intergenic |
| TAGGGCACAGATGGATTTCT+TGG | 0.326909 | 5.4:+67583797 | MS.gene070514:CDS |
| AAAATGAAAGGTATCATAAA+AGG | 0.343594 | 5.4:+67583715 | MS.gene070514:CDS |
| TAAGCATAGTGAAGAATTAT+CGG | 0.403413 | 5.4:+67583872 | MS.gene070514:CDS |
| CATAAAAGGCTGCGTAGCTA+TGG | 0.410348 | 5.4:+67583729 | MS.gene070514:CDS |
| TGACGAGTGTCATCACTTAT+CGG | 0.419794 | 5.4:-67583396 | None:intergenic |
| ACACTTACACCGCATATACT+TGG | 0.430867 | 5.4:+67583771 | MS.gene070514:CDS |
| AAAAGAATTAGATGGGAAAG+AGG | 0.438382 | 5.4:+67583506 | MS.gene070514:CDS |
| GGCACAGATGGATTTCTTGG+TGG | 0.464524 | 5.4:+67583800 | MS.gene070514:CDS |
| TGACACTCGTCAAGCTCATA+AGG | 0.481324 | 5.4:+67583407 | MS.gene070514:CDS |
| AAAGAATTAGATGGGAAAGA+GGG | 0.492739 | 5.4:+67583507 | MS.gene070514:CDS |
| CACAAGTGTTACGAGAAAGA+TGG | 0.496565 | 5.4:+67583549 | MS.gene070514:CDS |
| TATACTTATACAGATAAAGA+AGG | 0.512321 | 5.4:+67583654 | MS.gene070514:CDS |
| CATCTTAAAAGTATTTATGT+AGG | 0.512889 | 5.4:+67583351 | MS.gene070514:CDS |
| GGGAAAGAGGGAGAAAAGAA+AGG | 0.514345 | 5.4:+67583519 | MS.gene070514:CDS |
| ACTTGGAAGTAGGGCACAGA+TGG | 0.526284 | 5.4:+67583788 | MS.gene070514:CDS |
| ATAAAAGGCTGCGTAGCTAT+GGG | 0.544718 | 5.4:+67583730 | MS.gene070514:CDS |
| ACAAGTGTTACGAGAAAGAT+GGG | 0.545256 | 5.4:+67583550 | MS.gene070514:CDS |
| ACCGCATATACTTGGAAGTA+GGG | 0.554688 | 5.4:+67583779 | MS.gene070514:CDS |
| TATATATGCTTAACAGTGCA+AGG | 0.561224 | 5.4:+67583690 | MS.gene070514:CDS |
| CACCGCATATACTTGGAAGT+AGG | 0.563765 | 5.4:+67583778 | MS.gene070514:CDS |
| ACACATATTCTTATTCTGAA+AGG | 0.569596 | 5.4:-67583896 | None:intergenic |
| CAGTGCAAGGAGAAAATGAA+AGG | 0.589149 | 5.4:+67583703 | MS.gene070514:CDS |
| ATACTTATACAGATAAAGAA+GGG | 0.623826 | 5.4:+67583655 | MS.gene070514:CDS |
| GCCCTACTTCCAAGTATATG+CGG | 0.644753 | 5.4:-67583780 | None:intergenic |
| TAGCTATGGGAAGTCAGAGG+AGG | 0.673118 | 5.4:+67583743 | MS.gene070514:CDS |
| GCGTAGCTATGGGAAGTCAG+AGG | 0.678639 | 5.4:+67583740 | MS.gene070514:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAGTAGAAAAAGAATTAGA+TGG | + | chr5.4:67583498-67583517 | MS.gene070514:CDS | 20.0% |
| !! | AAGTAGAAAAAGAATTAGAT+GGG | + | chr5.4:67583499-67583518 | MS.gene070514:CDS | 20.0% |
| !! | ATACTTATACAGATAAAGAA+GGG | + | chr5.4:67583655-67583674 | MS.gene070514:CDS | 20.0% |
| !! | TATACTTATACAGATAAAGA+AGG | + | chr5.4:67583654-67583673 | MS.gene070514:CDS | 20.0% |
| !!! | AAAATGAAAGGTATCATAAA+AGG | + | chr5.4:67583715-67583734 | MS.gene070514:CDS | 20.0% |
| !!! | CATCTTAAAAGTATTTATGT+AGG | + | chr5.4:67583351-67583370 | MS.gene070514:CDS | 20.0% |
| !!! | CTACATAAATACTTTTAAGA+TGG | - | chr5.4:67583353-67583372 | None:intergenic | 20.0% |
| !!! | TGATATTTTCTTCAATTTCT+CGG | - | chr5.4:67583580-67583599 | None:intergenic | 20.0% |
| ! | TAAGCATAGTGAAGAATTAT+CGG | + | chr5.4:67583872-67583891 | MS.gene070514:CDS | 25.0% |
| !! | AACGAATTTTACACAAAGAA+TGG | - | chr5.4:67583323-67583342 | None:intergenic | 25.0% |
| !!! | AAATAATAGAGTGTCTTTTG+AGG | + | chr5.4:67583842-67583861 | MS.gene070514:CDS | 25.0% |
| !!! | AAGGTAATGATTATTGATTC+AGG | + | chr5.4:67583426-67583445 | MS.gene070514:CDS | 25.0% |
| AAAAGAATTAGATGGGAAAG+AGG | + | chr5.4:67583506-67583525 | MS.gene070514:CDS | 30.0% | |
| AAAGAATTAGATGGGAAAGA+GGG | + | chr5.4:67583507-67583526 | MS.gene070514:CDS | 30.0% | |
| TATATATGCTTAACAGTGCA+AGG | + | chr5.4:67583690-67583709 | MS.gene070514:CDS | 30.0% | |
| !! | AGACAAAAATTGAGTTGAAG+AGG | + | chr5.4:67583613-67583632 | MS.gene070514:CDS | 30.0% |
| !! | GACAAAAATTGAGTTGAAGA+GGG | + | chr5.4:67583614-67583633 | MS.gene070514:CDS | 30.0% |
| ACAAGTGTTACGAGAAAGAT+GGG | + | chr5.4:67583550-67583569 | MS.gene070514:CDS | 35.0% | |
| ! | GAATGGTCTTGATCTGTTAA+AGG | - | chr5.4:67583306-67583325 | None:intergenic | 35.0% |
| ACACTTACACCGCATATACT+TGG | + | chr5.4:67583771-67583790 | MS.gene070514:CDS | 40.0% | |
| ACCGCATATACTTGGAAGTA+GGG | + | chr5.4:67583779-67583798 | MS.gene070514:CDS | 40.0% | |
| ATAAAAGGCTGCGTAGCTAT+GGG | + | chr5.4:67583730-67583749 | MS.gene070514:CDS | 40.0% | |
| CACAAGTGTTACGAGAAAGA+TGG | + | chr5.4:67583549-67583568 | MS.gene070514:CDS | 40.0% | |
| CAGTGCAAGGAGAAAATGAA+AGG | + | chr5.4:67583703-67583722 | MS.gene070514:CDS | 40.0% | |
| TGACGAGTGTCATCACTTAT+CGG | - | chr5.4:67583399-67583418 | None:intergenic | 40.0% | |
| CACCGCATATACTTGGAAGT+AGG | + | chr5.4:67583778-67583797 | MS.gene070514:CDS | 45.0% | |
| CATAAAAGGCTGCGTAGCTA+TGG | + | chr5.4:67583729-67583748 | MS.gene070514:CDS | 45.0% | |
| GCCCTACTTCCAAGTATATG+CGG | - | chr5.4:67583783-67583802 | None:intergenic | 45.0% | |
| GGGAAAGAGGGAGAAAAGAA+AGG | + | chr5.4:67583519-67583538 | MS.gene070514:CDS | 45.0% | |
| TGACACTCGTCAAGCTCATA+AGG | + | chr5.4:67583407-67583426 | MS.gene070514:CDS | 45.0% | |
| ! | TAGGGCACAGATGGATTTCT+TGG | + | chr5.4:67583797-67583816 | MS.gene070514:CDS | 45.0% |
| !! | AGCAAGTTCACTTTTGGGCA+CGG | + | chr5.4:67583252-67583271 | MS.gene070514:CDS | 45.0% |
| ACTTGGAAGTAGGGCACAGA+TGG | + | chr5.4:67583788-67583807 | MS.gene070514:CDS | 50.0% | |
| TAGCTATGGGAAGTCAGAGG+AGG | + | chr5.4:67583743-67583762 | MS.gene070514:CDS | 50.0% | |
| ! | GGCACAGATGGATTTCTTGG+TGG | + | chr5.4:67583800-67583819 | MS.gene070514:CDS | 50.0% |
| GCGTAGCTATGGGAAGTCAG+AGG | + | chr5.4:67583740-67583759 | MS.gene070514:CDS | 55.0% | |
| !! | GTTCACTTTTGGGCACGGTG+TGG | + | chr5.4:67583257-67583276 | MS.gene070514:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.4 | gene | 67583249 | 67583908 | 67583249 | ID=MS.gene070514 |
| chr5.4 | mRNA | 67583249 | 67583908 | 67583249 | ID=MS.gene070514.t1;Parent=MS.gene070514 |
| chr5.4 | exon | 67583249 | 67583908 | 67583249 | ID=MS.gene070514.t1.exon1;Parent=MS.gene070514.t1 |
| chr5.4 | CDS | 67583249 | 67583908 | 67583249 | ID=cds.MS.gene070514.t1;Parent=MS.gene070514.t1 |
| Gene Sequence |
| Protein sequence |
