Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene070534.t1 | XP_003616876.2 | 99.7 | 677 | 2 | 0 | 1 | 677 | 1 | 677 | 0.00E+00 | 1362.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene070534.t1 | P93471 | 93.9 | 677 | 36 | 1 | 1 | 677 | 1 | 672 | 0.0e+00 | 1275.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene070534.t1 | G7KH91 | 99.7 | 677 | 2 | 0 | 1 | 677 | 1 | 677 | 0.0e+00 | 1362.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene057668 | MS.gene070534 | 0.852846 | 3.53E-61 | -1.69E-46 |
| MS.gene058075 | MS.gene070534 | 0.803368 | 3.60E-49 | -1.69E-46 |
| MS.gene059674 | MS.gene070534 | 0.817894 | 2.60E-52 | -1.69E-46 |
| MS.gene059807 | MS.gene070534 | 0.828865 | 7.09E-55 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene070534.t1 | MTR_5g085250 | 99.705 | 677 | 2 | 0 | 1 | 677 | 1 | 677 | 0.0 | 1415 |
| MS.gene070534.t1 | MTR_5g085250 | 99.817 | 545 | 1 | 0 | 133 | 677 | 29 | 573 | 0.0 | 1134 |
| MS.gene070534.t1 | MTR_5g085250 | 99.815 | 540 | 1 | 0 | 138 | 677 | 1 | 540 | 0.0 | 1125 |
| MS.gene070534.t1 | MTR_2g085210 | 49.138 | 348 | 170 | 2 | 335 | 675 | 527 | 874 | 5.99e-114 | 363 |
| MS.gene070534.t1 | MTR_5g009530 | 47.688 | 346 | 169 | 3 | 341 | 675 | 689 | 1033 | 2.02e-106 | 347 |
| MS.gene070534.t1 | MTR_8g027985 | 44.886 | 352 | 181 | 3 | 336 | 675 | 613 | 963 | 1.43e-101 | 332 |
| MS.gene070534.t1 | MTR_5g009530 | 48.951 | 286 | 138 | 1 | 341 | 618 | 689 | 974 | 1.19e-90 | 303 |
| MS.gene070534.t1 | MTR_2g085210 | 50.307 | 163 | 81 | 0 | 335 | 497 | 527 | 689 | 2.05e-48 | 181 |
| MS.gene070534.t1 | MTR_2g085210 | 50.307 | 163 | 81 | 0 | 335 | 497 | 527 | 689 | 2.50e-48 | 181 |
| MS.gene070534.t1 | MTR_5g009530 | 46.281 | 121 | 64 | 1 | 341 | 461 | 689 | 808 | 8.98e-29 | 123 |
| MS.gene070534.t1 | MTR_6g004040 | 27.273 | 187 | 128 | 6 | 452 | 634 | 1 | 183 | 8.00e-13 | 72.4 |
| MS.gene070534.t1 | MTR_5g016370 | 21.277 | 329 | 221 | 10 | 356 | 673 | 290 | 591 | 1.01e-12 | 71.6 |
| MS.gene070534.t1 | MTR_1g072140 | 30.058 | 173 | 111 | 6 | 427 | 593 | 116 | 284 | 1.15e-12 | 70.1 |
| MS.gene070534.t1 | MTR_7g086360 | 26.042 | 192 | 131 | 6 | 437 | 619 | 112 | 301 | 1.06e-11 | 67.4 |
| MS.gene070534.t1 | MTR_4g073080 | 29.268 | 164 | 105 | 4 | 428 | 581 | 168 | 330 | 1.66e-11 | 67.0 |
| MS.gene070534.t1 | MTR_4g095058 | 22.086 | 326 | 209 | 11 | 364 | 673 | 278 | 574 | 6.98e-11 | 65.9 |
| MS.gene070534.t1 | MTR_4g095058 | 22.086 | 326 | 209 | 11 | 364 | 673 | 276 | 572 | 7.92e-11 | 65.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene070534.t1 | AT2G32950 | 77.500 | 680 | 145 | 6 | 1 | 677 | 1 | 675 | 0.0 | 1095 |
| MS.gene070534.t1 | AT2G32950 | 80.657 | 548 | 100 | 4 | 132 | 677 | 19 | 562 | 0.0 | 920 |
| MS.gene070534.t1 | AT3G15354 | 36.508 | 567 | 290 | 9 | 148 | 673 | 54 | 591 | 3.24e-115 | 358 |
| MS.gene070534.t1 | AT3G15354 | 36.508 | 567 | 290 | 9 | 148 | 673 | 54 | 591 | 3.24e-115 | 358 |
| MS.gene070534.t1 | AT1G53090 | 46.849 | 365 | 185 | 3 | 316 | 673 | 208 | 570 | 1.03e-114 | 356 |
| MS.gene070534.t1 | AT3G15354 | 36.508 | 567 | 290 | 9 | 148 | 673 | 304 | 841 | 6.22e-112 | 357 |
| MS.gene070534.t1 | AT1G53090 | 46.849 | 365 | 185 | 3 | 316 | 673 | 429 | 791 | 6.95e-112 | 355 |
| MS.gene070534.t1 | AT1G53090 | 46.849 | 365 | 185 | 3 | 316 | 673 | 429 | 791 | 6.95e-112 | 355 |
| MS.gene070534.t1 | AT1G53090 | 46.849 | 365 | 185 | 3 | 316 | 673 | 429 | 791 | 6.95e-112 | 355 |
| MS.gene070534.t1 | AT4G11110 | 48.071 | 337 | 165 | 2 | 349 | 675 | 699 | 1035 | 3.02e-105 | 343 |
| MS.gene070534.t1 | AT3G15354 | 35.626 | 567 | 287 | 10 | 148 | 673 | 304 | 833 | 7.62e-105 | 338 |
| MS.gene070534.t1 | AT2G46340 | 37.571 | 527 | 279 | 9 | 160 | 675 | 541 | 1028 | 1.43e-104 | 342 |
| MS.gene070534.t1 | AT2G46340 | 37.571 | 527 | 279 | 9 | 160 | 675 | 541 | 1028 | 1.43e-104 | 342 |
| MS.gene070534.t1 | AT5G23730 | 35.061 | 328 | 181 | 11 | 374 | 674 | 41 | 363 | 1.77e-57 | 199 |
| MS.gene070534.t1 | AT5G52250 | 33.437 | 323 | 182 | 12 | 372 | 673 | 70 | 380 | 1.38e-48 | 175 |
| MS.gene070534.t1 | AT1G53090 | 45.604 | 182 | 97 | 1 | 316 | 497 | 429 | 608 | 2.73e-48 | 180 |
| MS.gene070534.t1 | AT3G15354 | 30.729 | 384 | 203 | 7 | 148 | 497 | 304 | 658 | 1.01e-47 | 179 |
| MS.gene070534.t1 | AT3G15610 | 26.906 | 223 | 146 | 7 | 367 | 585 | 93 | 302 | 1.49e-13 | 73.2 |
| MS.gene070534.t1 | AT5G08390 | 28.492 | 179 | 122 | 5 | 458 | 634 | 9 | 183 | 2.03e-13 | 74.3 |
| MS.gene070534.t1 | AT4G35050 | 24.242 | 198 | 137 | 6 | 396 | 581 | 141 | 337 | 6.89e-13 | 71.6 |
| MS.gene070534.t1 | AT4G18900 | 27.488 | 211 | 140 | 6 | 421 | 619 | 233 | 442 | 1.39e-12 | 70.9 |
| MS.gene070534.t1 | AT5G23430 | 27.624 | 181 | 125 | 5 | 452 | 628 | 1 | 179 | 1.47e-12 | 71.6 |
| MS.gene070534.t1 | AT5G23430 | 27.624 | 181 | 125 | 5 | 452 | 628 | 1 | 179 | 1.47e-12 | 71.6 |
| MS.gene070534.t1 | AT5G23430 | 27.624 | 181 | 125 | 5 | 452 | 628 | 1 | 179 | 1.47e-12 | 71.6 |
| MS.gene070534.t1 | AT2G43770 | 25.907 | 193 | 132 | 6 | 436 | 619 | 109 | 299 | 2.72e-12 | 69.3 |
| MS.gene070534.t1 | AT5G58230 | 25.620 | 242 | 156 | 9 | 357 | 581 | 115 | 349 | 4.88e-12 | 68.9 |
| MS.gene070534.t1 | AT4G35370 | 29.146 | 199 | 133 | 5 | 428 | 618 | 222 | 420 | 1.74e-11 | 67.4 |
| MS.gene070534.t1 | AT3G49660 | 25.743 | 202 | 140 | 7 | 427 | 622 | 118 | 315 | 1.87e-11 | 66.2 |
| MS.gene070534.t1 | AT1G52730 | 25.225 | 222 | 152 | 6 | 367 | 584 | 93 | 304 | 3.26e-11 | 65.9 |
| MS.gene070534.t1 | AT1G52730 | 25.225 | 222 | 152 | 6 | 367 | 584 | 93 | 304 | 3.26e-11 | 65.9 |
| MS.gene070534.t1 | AT2G16780 | 23.737 | 198 | 138 | 6 | 396 | 581 | 140 | 336 | 5.83e-11 | 65.5 |
| MS.gene070534.t1 | AT1G11160 | 26.554 | 177 | 124 | 5 | 458 | 632 | 8 | 180 | 6.80e-11 | 66.2 |
| MS.gene070534.t1 | AT1G11160 | 26.554 | 177 | 124 | 5 | 458 | 632 | 8 | 180 | 6.91e-11 | 66.2 |
| MS.gene070534.t1 | AT1G11160 | 26.554 | 177 | 124 | 5 | 458 | 632 | 8 | 180 | 7.10e-11 | 66.2 |
| MS.gene070534.t1 | AT3G15980 | 26.749 | 243 | 153 | 8 | 438 | 672 | 30 | 255 | 7.81e-11 | 66.2 |
| MS.gene070534.t1 | AT1G61210 | 24.294 | 177 | 130 | 4 | 458 | 632 | 8 | 182 | 8.07e-11 | 66.2 |
| MS.gene070534.t1 | AT3G15980 | 26.749 | 243 | 153 | 8 | 438 | 672 | 30 | 255 | 8.27e-11 | 65.9 |
| MS.gene070534.t1 | AT3G15980 | 26.749 | 243 | 153 | 8 | 438 | 672 | 30 | 255 | 8.82e-11 | 65.9 |
| MS.gene070534.t1 | AT3G15980 | 26.749 | 243 | 153 | 8 | 438 | 672 | 30 | 255 | 8.82e-11 | 65.9 |
| MS.gene070534.t1 | AT3G15980 | 26.749 | 243 | 153 | 8 | 438 | 672 | 30 | 255 | 8.82e-11 | 65.9 |
| MS.gene070534.t1 | AT3G15980 | 26.749 | 243 | 153 | 8 | 438 | 672 | 30 | 255 | 8.82e-11 | 65.9 |
| MS.gene070534.t1 | AT3G15980 | 26.749 | 243 | 153 | 8 | 438 | 672 | 30 | 255 | 9.99e-11 | 65.9 |
Find 150 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTTCCTTTGGCAGAGTTTAA+TGG | 0.237138 | 5.4:+67313412 | MS.gene070534:intron |
| CAGATAGTACACTGCGGTTA+TGG | 0.252524 | 5.4:+67314480 | MS.gene070534:CDS |
| AACCCTCCTCGAATATTTAA+AGG | 0.258388 | 5.4:-67308721 | None:intergenic |
| ATTCGAGGAGGGTTGTCTTC+AGG | 0.287880 | 5.4:+67308730 | MS.gene070534:CDS |
| TTTGTAGATAACATTCTTGT+AGG | 0.307788 | 5.4:-67309927 | None:intergenic |
| TTGCGGATGCTTGATGATTC+TGG | 0.320860 | 5.4:+67308640 | MS.gene070534:CDS |
| TATTTCTCAAATCATAATAA+TGG | 0.348001 | 5.4:-67314367 | None:intergenic |
| ACTTCATCAGTGCTGTTTGC+TGG | 0.354397 | 5.4:+67315220 | MS.gene070534:CDS |
| TCCGAATTTCTTACTCGATA+AGG | 0.355252 | 5.4:+67304628 | MS.gene070534:CDS |
| CACTGATGGGATCTCTTCTC+TGG | 0.356789 | 5.4:-67308810 | None:intergenic |
| ATGAAGGAATTGTGACTGTT+TGG | 0.358639 | 5.4:+67313245 | MS.gene070534:CDS |
| ATTCGGAAACAAATTACTAT+TGG | 0.363357 | 5.4:-67304612 | None:intergenic |
| CAAAGTTTGGTGCACGAATC+AGG | 0.364240 | 5.4:+67313774 | MS.gene070534:CDS |
| TTGACTATTTGCAGTTAGTA+TGG | 0.366718 | 5.4:-67315259 | None:intergenic |
| AATTCGTATCTCTTTCTTGT+TGG | 0.388471 | 5.4:-67309981 | None:intergenic |
| TTCACAGTATATAAATCAAT+CGG | 0.394235 | 5.4:+67308840 | MS.gene070534:CDS |
| AGCAAGCACCAGCACTTTGA+TGG | 0.401646 | 5.4:-67315286 | None:intergenic |
| TTCGAGGAGGGTTGTCTTCA+GGG | 0.403288 | 5.4:+67308731 | MS.gene070534:CDS |
| AGATAGTACACTGCGGTTAT+GGG | 0.404349 | 5.4:+67314481 | MS.gene070534:CDS |
| CAATGTCCTCCTTTATGAAC+TGG | 0.413835 | 5.4:-67308558 | None:intergenic |
| TCTTTGAGCAGGTCAAAGTT+TGG | 0.423567 | 5.4:+67313761 | MS.gene070534:intron |
| TGAAGGAATTGTGACTGTTT+GGG | 0.426678 | 5.4:+67313246 | MS.gene070534:CDS |
| AGTTCATAAAGGAGGACATT+GGG | 0.430648 | 5.4:+67308560 | MS.gene070534:CDS |
| ATTCAGCCAACATCGTTTCA+AGG | 0.444771 | 5.4:+67311098 | MS.gene070534:CDS |
| CTATTTGCAGTTAGTATGGT+GGG | 0.447922 | 5.4:-67315255 | None:intergenic |
| GACGATTTGTTTGCTACAGC+TGG | 0.448381 | 5.4:+67311851 | MS.gene070534:CDS |
| TGTGTATTATTACTCATCTT+CGG | 0.448436 | 5.4:+67304552 | MS.gene070534:CDS |
| CAAAACTTAGTTGCTTGAGT+TGG | 0.449682 | 5.4:+67313185 | MS.gene070534:CDS |
| GTGAAGTATAATCCTGGATC+GGG | 0.450446 | 5.4:+67313841 | MS.gene070534:CDS |
| TCCTCCGCATCATCCATGTC+AGG | 0.456144 | 5.4:-67315183 | None:intergenic |
| TCCGGTGACGGTTACGGTGG+TGG | 0.456824 | 5.4:-67304399 | None:intergenic |
| GACGCGTTTCTTACAGCTTG+TGG | 0.459464 | 5.4:+67304512 | MS.gene070534:CDS |
| GGTTCGGGTTTGACGACGGC+GGG | 0.460222 | 5.4:-67304362 | None:intergenic |
| AACTGATCTCCAGTTCATAA+AGG | 0.467802 | 5.4:+67308549 | MS.gene070534:CDS |
| ACCTTATCGAGTAAGAAATT+CGG | 0.468460 | 5.4:-67304629 | None:intergenic |
| ACCCTCCTCGAATATTTAAA+GGG | 0.470850 | 5.4:-67308720 | None:intergenic |
| AAAGGAGGACATTGGGGCCG+TGG | 0.471087 | 5.4:+67308567 | MS.gene070534:CDS |
| ACTCCGTCATGGTTGCGATC+GGG | 0.471862 | 5.4:-67304445 | None:intergenic |
| AAGTCTCAAATCAGCTCTCA+TGG | 0.472937 | 5.4:+67308778 | MS.gene070534:CDS |
| TTAATAATCTGCATACAAAT+CGG | 0.475950 | 5.4:-67304488 | None:intergenic |
| AAAATCATGGCATTCATCAA+TGG | 0.479333 | 5.4:+67308669 | MS.gene070534:CDS |
| TCTCAGCAAGGAGTGACAAA+AGG | 0.479384 | 5.4:-67308062 | None:intergenic |
| TGACAACTTTCACACGCTAC+AGG | 0.480406 | 5.4:+67310055 | MS.gene070534:CDS |
| GTCTCCGCCGGCGATGTCTC+CGG | 0.482253 | 5.4:-67304417 | None:intergenic |
| GCAATGTAATTCCCCGATCC+AGG | 0.483126 | 5.4:-67313853 | None:intergenic |
| CGTGTGGTCATCTCCACAAC+AGG | 0.491234 | 5.4:-67313160 | None:intergenic |
| TCTTGTGCCCACTGAAAACA+TGG | 0.491272 | 5.4:-67314403 | None:intergenic |
| TGTATTCACTACTTACTGTA+AGG | 0.494510 | 5.4:-67314997 | None:intergenic |
| TGCCCACTGAAAACATGGAC+TGG | 0.495346 | 5.4:-67314398 | None:intergenic |
| GAAACAAATTACTATTGGTA+AGG | 0.495996 | 5.4:-67304607 | None:intergenic |
| GCCTGCCGGAAATGCTCCAC+GGG | 0.497681 | 5.4:-67305600 | None:intergenic |
| GATCCCTCTATGCTTGTATC+CGG | 0.500871 | 5.4:+67313485 | MS.gene070534:CDS |
| GCCGGCGATGTCTCCGGTGA+CGG | 0.503367 | 5.4:-67304411 | None:intergenic |
| ATCCGGTAGTGATGATTGTA+AGG | 0.506760 | 5.4:+67313502 | MS.gene070534:CDS |
| GATGTCTCCGGTGACGGTTA+CGG | 0.509729 | 5.4:-67304405 | None:intergenic |
| ACTTATATCGTGCTCGGGAT+CGG | 0.512625 | 5.4:+67308605 | MS.gene070534:CDS |
| TAGATTTAGTTCCCCTGACA+TGG | 0.513170 | 5.4:+67315170 | MS.gene070534:CDS |
| CAAATCAGCTCTCATGGACA+TGG | 0.515444 | 5.4:+67308784 | MS.gene070534:CDS |
| TTTCCGGCAGGCGATACAAA+AGG | 0.515478 | 5.4:+67305611 | MS.gene070534:CDS |
| CTCAGCAAGGAGTGACAAAA+GGG | 0.516560 | 5.4:-67308061 | None:intergenic |
| TCCACCACCGTAACCGTCAC+CGG | 0.517126 | 5.4:+67304398 | MS.gene070534:CDS |
| GAATGGACTTATATCGTGCT+CGG | 0.518337 | 5.4:+67308599 | MS.gene070534:CDS |
| CCCCGTGGAGCATTTCCGGC+AGG | 0.521449 | 5.4:+67305599 | MS.gene070534:CDS |
| AACTCCGTCATGGTTGCGAT+CGG | 0.521967 | 5.4:-67304446 | None:intergenic |
| ACTATTTGCAGTTAGTATGG+TGG | 0.523504 | 5.4:-67315256 | None:intergenic |
| CGCCTGCCGGAAATGCTCCA+CGG | 0.524040 | 5.4:-67305601 | None:intergenic |
| CTGTGAATCTGATCCACTGA+TGG | 0.527140 | 5.4:-67308824 | None:intergenic |
| AAATCTCCAAGCCTCTGACT+TGG | 0.530640 | 5.4:+67315145 | MS.gene070534:CDS |
| AATCATAATAATGGATGTGA+TGG | 0.531014 | 5.4:-67314358 | None:intergenic |
| CATGTCTATATTTAAAACAC+TGG | 0.531620 | 5.4:-67313800 | None:intergenic |
| TCTAGCCCTTTAAATATTCG+AGG | 0.534972 | 5.4:+67308715 | MS.gene070534:CDS |
| CATGGATGATGCGGAGGATG+AGG | 0.535745 | 5.4:+67315188 | MS.gene070534:CDS |
| GATGATGCGGAGGATGAGGC+TGG | 0.535882 | 5.4:+67315192 | MS.gene070534:CDS |
| TACCTTACAATCATCACTAC+CGG | 0.541446 | 5.4:-67313504 | None:intergenic |
| TGCTGATGCAAGTTCATCGT+TGG | 0.542829 | 5.4:-67314453 | None:intergenic |
| AACTCACCTTGAAACGATGT+TGG | 0.543525 | 5.4:-67311104 | None:intergenic |
| AATGGACTTATATCGTGCTC+GGG | 0.547396 | 5.4:+67308600 | MS.gene070534:CDS |
| TCACAGTATATAAATCAATC+GGG | 0.550556 | 5.4:+67308841 | MS.gene070534:CDS |
| TCAAGGCACCATCAAAGTGC+TGG | 0.553897 | 5.4:+67315278 | MS.gene070534:CDS |
| TGGTTCGGGTTTGACGACGG+CGG | 0.555209 | 5.4:-67304363 | None:intergenic |
| TCCGACGGAGTGTTCTTCCA+TGG | 0.556975 | 5.4:-67304330 | None:intergenic |
| GCCCTTTAAATATTCGAGGA+GGG | 0.557638 | 5.4:+67308719 | MS.gene070534:CDS |
| TGTGAAGTATAATCCTGGAT+CGG | 0.558008 | 5.4:+67313840 | MS.gene070534:CDS |
| TCTATGCCAAGTCAGAGGCT+TGG | 0.560666 | 5.4:-67315151 | None:intergenic |
| GTCTTTATCTAACTCCGTCA+TGG | 0.561898 | 5.4:-67304456 | None:intergenic |
| ATCGGTACTCTGTGAAGTTG+CGG | 0.565158 | 5.4:+67308623 | MS.gene070534:CDS |
| TGTTGTGTGAAGTATAATCC+TGG | 0.566028 | 5.4:+67313835 | MS.gene070534:CDS |
| TCTGCATACAAATCGGACAT+AGG | 0.566486 | 5.4:-67304481 | None:intergenic |
| AACAGGACAATGAGCATCTG+TGG | 0.567948 | 5.4:-67313143 | None:intergenic |
| GGATGGTTCGGGTTTGACGA+CGG | 0.571721 | 5.4:-67304366 | None:intergenic |
| AAGAGCATGAAAAGCGTGCA+TGG | 0.573190 | 5.4:+67313441 | MS.gene070534:CDS |
| CACTGAAAACATGGACTGGA+CGG | 0.574289 | 5.4:-67314394 | None:intergenic |
| GGCCGTGGAGAAACATAGAA+TGG | 0.575022 | 5.4:+67308582 | MS.gene070534:CDS |
| ACGAATTTCATAAGTCGAGA+AGG | 0.577292 | 5.4:+67309997 | MS.gene070534:CDS |
| CCTCCGCATCATCCATGTCA+GGG | 0.577657 | 5.4:-67315182 | None:intergenic |
| TATTTAAAGGGCTAGATGCA+AGG | 0.578156 | 5.4:-67308708 | None:intergenic |
| AAAGAAGAGGGTGCATACAC+AGG | 0.578452 | 5.4:+67308879 | MS.gene070534:CDS |
| GTACAGGTCCGCACTTTCAA+AGG | 0.579062 | 5.4:+67314947 | MS.gene070534:intron |
| TTGCTCTAGTTAGAAAGAAG+AGG | 0.582886 | 5.4:+67308866 | MS.gene070534:CDS |
| ATAGCTAGTAGTGATTATGA+AGG | 0.589727 | 5.4:+67313229 | MS.gene070534:CDS |
| AGATGCTCATTGTCCTGTTG+TGG | 0.590252 | 5.4:+67313147 | MS.gene070534:CDS |
| TTCCCCTGACATGGATGATG+CGG | 0.590370 | 5.4:+67315179 | MS.gene070534:CDS |
| TTGACGACGGCGGGAACGAG+TGG | 0.591311 | 5.4:-67304353 | None:intergenic |
| GTCCATTCTATGTTTCTCCA+CGG | 0.592829 | 5.4:-67308584 | None:intergenic |
| CAGGCTGCAGATAAGCCACA+TGG | 0.596225 | 5.4:+67309958 | MS.gene070534:CDS |
| ACCGTCACCGGAGACATCGC+CGG | 0.598356 | 5.4:+67304410 | MS.gene070534:CDS |
| TCTCTTTCTTGTTGGCCATG+TGG | 0.598443 | 5.4:-67309973 | None:intergenic |
| AGCCCTTTAAATATTCGAGG+AGG | 0.599877 | 5.4:+67308718 | MS.gene070534:CDS |
| GGCGGGAACGAGTGGTCCGA+CGG | 0.601510 | 5.4:-67304345 | None:intergenic |
| CTACCGGATACAAGCATAGA+GGG | 0.604059 | 5.4:-67313488 | None:intergenic |
| TTTCCCGATCGCAACCATGA+CGG | 0.604297 | 5.4:+67304442 | MS.gene070534:CDS |
| TGCTCTAGTTAGAAAGAAGA+GGG | 0.610073 | 5.4:+67308867 | MS.gene070534:CDS |
| TATTTGCAGTTAGTATGGTG+GGG | 0.611419 | 5.4:-67315254 | None:intergenic |
| CCCTGACATGGATGATGCGG+AGG | 0.614289 | 5.4:+67315182 | MS.gene070534:CDS |
| TTGGGATGTGACCACTCGAA+AGG | 0.618691 | 5.4:+67313264 | MS.gene070534:CDS |
| TAGCGTGGCCTTTGAAAGTG+CGG | 0.621553 | 5.4:-67314955 | None:intergenic |
| ACTACCGGATACAAGCATAG+AGG | 0.622223 | 5.4:-67313489 | None:intergenic |
| TGACGGTTACGGTGGTGGAG+AGG | 0.622505 | 5.4:-67304394 | None:intergenic |
| ATACTAACTGCAAATAGTCA+AGG | 0.622948 | 5.4:+67315261 | MS.gene070534:CDS |
| ATTCTGGTGGAAGAAAATCA+TGG | 0.624185 | 5.4:+67308656 | MS.gene070534:CDS |
| GATCGGGAAATTGTCTCCGC+CGG | 0.625022 | 5.4:-67304429 | None:intergenic |
| CTAAATCTATGCCAAGTCAG+AGG | 0.627688 | 5.4:-67315156 | None:intergenic |
| CGGATGCTTGATGATTCTGG+TGG | 0.629544 | 5.4:+67308643 | MS.gene070534:CDS |
| CAGAGAAGAGATCCCATCAG+TGG | 0.630890 | 5.4:+67308811 | MS.gene070534:CDS |
| GTTCACAATTACCTTTCGAG+TGG | 0.633179 | 5.4:-67313275 | None:intergenic |
| TGAAGTATTTGTCTACCACA+AGG | 0.636400 | 5.4:+67315042 | MS.gene070534:CDS |
| CAGTTCATAAAGGAGGACAT+TGG | 0.639000 | 5.4:+67308559 | MS.gene070534:CDS |
| TGTGAATCTGATCCACTGAT+GGG | 0.640014 | 5.4:-67308823 | None:intergenic |
| GTCTCCGGTGACGGTTACGG+TGG | 0.644114 | 5.4:-67304402 | None:intergenic |
| TGATCTCCAGTTCATAAAGG+AGG | 0.645464 | 5.4:+67308552 | MS.gene070534:CDS |
| ACAGGACAATGAGCATCTGT+GGG | 0.650389 | 5.4:-67313142 | None:intergenic |
| GTTCATAAAGGAGGACATTG+GGG | 0.653194 | 5.4:+67308561 | MS.gene070534:CDS |
| CTCCGCATCATCCATGTCAG+GGG | 0.653353 | 5.4:-67315181 | None:intergenic |
| TAGCAAACAAATCGTCATCA+CGG | 0.653471 | 5.4:-67311844 | None:intergenic |
| AGTCGAGAAGGTTACAGTGG+TGG | 0.654884 | 5.4:+67310009 | MS.gene070534:CDS |
| AGTAGTGAATACATTGCATG+TGG | 0.658370 | 5.4:+67315007 | MS.gene070534:CDS |
| GGGTTGTGAAGTGACAATGA+AGG | 0.664179 | 5.4:+67308029 | MS.gene070534:intron |
| ACCATGGAAGAACACTCCGT+CGG | 0.669086 | 5.4:+67304329 | None:intergenic |
| TCAATGGACAAGAATAGCAG+TGG | 0.671034 | 5.4:+67308685 | MS.gene070534:CDS |
| GTCATTGCAGAAATAAGACA+CGG | 0.676332 | 5.4:+67311064 | MS.gene070534:CDS |
| AAAAGTGATTGTCCTTGCTG+TGG | 0.679991 | 5.4:+67304578 | MS.gene070534:CDS |
| ATAAGTCGAGAAGGTTACAG+TGG | 0.684944 | 5.4:+67310006 | MS.gene070534:CDS |
| AAAAGTTGATGAGTTGAAGG+AGG | 0.690590 | 5.4:+67308164 | MS.gene070534:CDS |
| GTCACCGGAGACATCGCCGG+CGG | 0.697103 | 5.4:+67304413 | MS.gene070534:CDS |
| GTAAGGTAATGACCACAGCA+AGG | 0.699689 | 5.4:-67304590 | None:intergenic |
| CCTGCCGGAAATGCTCCACG+GGG | 0.700061 | 5.4:-67305599 | None:intergenic |
| TGAAGTATAATCCTGGATCG+GGG | 0.700073 | 5.4:+67313842 | MS.gene070534:CDS |
| AGTTTGGTGCACGAATCAGG+AGG | 0.729323 | 5.4:+67313777 | MS.gene070534:CDS |
| CTGCCGGAAATGCTCCACGG+GGG | 0.738551 | 5.4:-67305598 | None:intergenic |
| CATCAACAGATAGTACACTG+CGG | 0.845506 | 5.4:+67314474 | MS.gene070534:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.4 | gene | 67304332 | 67315314 | 67304332 | ID=MS.gene070534 |
| chr5.4 | mRNA | 67304332 | 67315314 | 67304332 | ID=MS.gene070534.t1;Parent=MS.gene070534 |
| chr5.4 | exon | 67304332 | 67304649 | 67304332 | ID=MS.gene070534.t1.exon1;Parent=MS.gene070534.t1 |
| chr5.4 | CDS | 67304332 | 67304649 | 67304332 | ID=cds.MS.gene070534.t1;Parent=MS.gene070534.t1 |
| chr5.4 | exon | 67305555 | 67305632 | 67305555 | ID=MS.gene070534.t1.exon2;Parent=MS.gene070534.t1 |
| chr5.4 | CDS | 67305555 | 67305632 | 67305555 | ID=cds.MS.gene070534.t1;Parent=MS.gene070534.t1 |
| chr5.4 | exon | 67308030 | 67308185 | 67308030 | ID=MS.gene070534.t1.exon3;Parent=MS.gene070534.t1 |
| chr5.4 | CDS | 67308030 | 67308185 | 67308030 | ID=cds.MS.gene070534.t1;Parent=MS.gene070534.t1 |
| chr5.4 | exon | 67308544 | 67308900 | 67308544 | ID=MS.gene070534.t1.exon4;Parent=MS.gene070534.t1 |
| chr5.4 | CDS | 67308544 | 67308900 | 67308544 | ID=cds.MS.gene070534.t1;Parent=MS.gene070534.t1 |
| chr5.4 | exon | 67309919 | 67310076 | 67309919 | ID=MS.gene070534.t1.exon5;Parent=MS.gene070534.t1 |
| chr5.4 | CDS | 67309919 | 67310076 | 67309919 | ID=cds.MS.gene070534.t1;Parent=MS.gene070534.t1 |
| chr5.4 | exon | 67311054 | 67311119 | 67311054 | ID=MS.gene070534.t1.exon6;Parent=MS.gene070534.t1 |
| chr5.4 | CDS | 67311054 | 67311119 | 67311054 | ID=cds.MS.gene070534.t1;Parent=MS.gene070534.t1 |
| chr5.4 | exon | 67311832 | 67311910 | 67311832 | ID=MS.gene070534.t1.exon7;Parent=MS.gene070534.t1 |
| chr5.4 | CDS | 67311832 | 67311910 | 67311832 | ID=cds.MS.gene070534.t1;Parent=MS.gene070534.t1 |
| chr5.4 | exon | 67313130 | 67313285 | 67313130 | ID=MS.gene070534.t1.exon8;Parent=MS.gene070534.t1 |
| chr5.4 | CDS | 67313130 | 67313285 | 67313130 | ID=cds.MS.gene070534.t1;Parent=MS.gene070534.t1 |
| chr5.4 | exon | 67313425 | 67313523 | 67313425 | ID=MS.gene070534.t1.exon9;Parent=MS.gene070534.t1 |
| chr5.4 | CDS | 67313425 | 67313523 | 67313425 | ID=cds.MS.gene070534.t1;Parent=MS.gene070534.t1 |
| chr5.4 | exon | 67313772 | 67313876 | 67313772 | ID=MS.gene070534.t1.exon10;Parent=MS.gene070534.t1 |
| chr5.4 | CDS | 67313772 | 67313876 | 67313772 | ID=cds.MS.gene070534.t1;Parent=MS.gene070534.t1 |
| chr5.4 | exon | 67314344 | 67314523 | 67314344 | ID=MS.gene070534.t1.exon11;Parent=MS.gene070534.t1 |
| chr5.4 | CDS | 67314344 | 67314523 | 67314344 | ID=cds.MS.gene070534.t1;Parent=MS.gene070534.t1 |
| chr5.4 | exon | 67314953 | 67315063 | 67314953 | ID=MS.gene070534.t1.exon12;Parent=MS.gene070534.t1 |
| chr5.4 | CDS | 67314953 | 67315063 | 67314953 | ID=cds.MS.gene070534.t1;Parent=MS.gene070534.t1 |
| chr5.4 | exon | 67315144 | 67315314 | 67315144 | ID=MS.gene070534.t1.exon13;Parent=MS.gene070534.t1 |
| chr5.4 | CDS | 67315144 | 67315314 | 67315144 | ID=cds.MS.gene070534.t1;Parent=MS.gene070534.t1 |
| Gene Sequence |
| Protein sequence |
