Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene070637.t1 | XP_003621252.1 | 87.6 | 202 | 25 | 0 | 1 | 202 | 1 | 202 | 7.20E-79 | 303.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene070637.t1 | Q93Z27 | 38.1 | 210 | 116 | 5 | 1 | 202 | 1 | 204 | 7.9e-26 | 118.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene070637.t1 | G7L207 | 87.6 | 202 | 25 | 0 | 1 | 202 | 1 | 202 | 5.2e-79 | 303.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene04998 | MS.gene070637 | 0.807281 | 5.46E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene070637.t1 | MTR_7g011010 | 91.089 | 202 | 18 | 0 | 1 | 202 | 1 | 202 | 2.89e-121 | 342 |
| MS.gene070637.t1 | MTR_5g025690 | 82.524 | 206 | 26 | 2 | 1 | 202 | 1 | 200 | 4.80e-113 | 321 |
| MS.gene070637.t1 | MTR_4g127560 | 68.306 | 183 | 56 | 2 | 22 | 202 | 16 | 198 | 1.07e-75 | 226 |
| MS.gene070637.t1 | MTR_2g023310 | 86.275 | 51 | 7 | 0 | 85 | 135 | 47 | 97 | 8.04e-23 | 88.2 |
| MS.gene070637.t1 | MTR_4g082050 | 46.296 | 108 | 55 | 2 | 91 | 198 | 35 | 139 | 1.55e-19 | 81.3 |
| MS.gene070637.t1 | MTR_7g074240 | 49.231 | 65 | 33 | 0 | 133 | 197 | 106 | 170 | 4.66e-13 | 65.1 |
| MS.gene070637.t1 | MTR_2g098890 | 27.551 | 196 | 117 | 6 | 25 | 198 | 11 | 203 | 1.01e-12 | 64.7 |
| MS.gene070637.t1 | MTR_4g115170 | 44.595 | 74 | 41 | 0 | 126 | 199 | 145 | 218 | 1.12e-12 | 64.7 |
| MS.gene070637.t1 | MTR_4g067270 | 43.284 | 67 | 38 | 0 | 133 | 199 | 77 | 143 | 3.42e-11 | 59.3 |
| MS.gene070637.t1 | MTR_3g088655 | 33.562 | 146 | 81 | 3 | 60 | 197 | 17 | 154 | 8.88e-11 | 58.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene070637.t1 | AT5G39670 | 43.868 | 212 | 101 | 5 | 1 | 202 | 1 | 204 | 4.63e-47 | 154 |
| MS.gene070637.t1 | AT3G29000 | 63.303 | 109 | 38 | 1 | 91 | 199 | 85 | 191 | 7.43e-41 | 137 |
| MS.gene070637.t1 | AT3G47480 | 54.545 | 66 | 29 | 1 | 133 | 198 | 115 | 179 | 1.17e-15 | 72.0 |
| MS.gene070637.t1 | AT4G20780 | 54.930 | 71 | 29 | 1 | 127 | 197 | 116 | 183 | 1.92e-15 | 71.6 |
| MS.gene070637.t1 | AT2G43290 | 42.697 | 89 | 50 | 1 | 110 | 198 | 120 | 207 | 3.64e-15 | 71.2 |
| MS.gene070637.t1 | AT5G44460 | 49.296 | 71 | 33 | 1 | 129 | 199 | 109 | 176 | 5.93e-15 | 70.1 |
| MS.gene070637.t1 | AT1G05990 | 47.692 | 65 | 34 | 0 | 135 | 199 | 80 | 144 | 2.38e-13 | 65.1 |
| MS.gene070637.t1 | AT3G59440 | 27.500 | 200 | 102 | 6 | 25 | 199 | 7 | 188 | 1.72e-12 | 63.9 |
| MS.gene070637.t1 | AT4G03290 | 46.875 | 64 | 34 | 0 | 135 | 198 | 82 | 145 | 1.85e-12 | 62.8 |
| MS.gene070637.t1 | AT3G07490 | 45.161 | 62 | 34 | 0 | 138 | 199 | 82 | 143 | 9.07e-12 | 60.8 |
Find 20 sgRNAs with CRISPR-Local
Find 30 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCAAAGAGTTATGTGTATTT+TGG | 0.154565 | 7.1:-84488193 | MS.gene070637:CDS |
| GAGAAAGTGGAGAGATTAAG+AGG | 0.249917 | 7.1:-84488407 | MS.gene070637:CDS |
| TGAAGCGGTGATGGCGAAAA+TGG | 0.258329 | 7.1:-84488376 | MS.gene070637:CDS |
| GAAAGCAACTCAACTTTGTT+TGG | 0.330887 | 7.1:-84488600 | MS.gene070637:CDS |
| CTTCTCTGTCAACTTCGTTC+CGG | 0.355688 | 7.1:-84488504 | MS.gene070637:CDS |
| TGATGAGAATGAGCCAAGTT+TGG | 0.401446 | 7.1:-84488283 | MS.gene070637:CDS |
| ATCTTCACTTCCTCCAAACT+TGG | 0.413419 | 7.1:+84488270 | None:intergenic |
| TTCCGGTGACTTAGAAGTAA+AGG | 0.466313 | 7.1:-84488487 | MS.gene070637:CDS |
| TCCGGTGACTTAGAAGTAAA+GGG | 0.483748 | 7.1:-84488486 | MS.gene070637:CDS |
| GAAGCGGTGATGGCGAAAAT+GGG | 0.492089 | 7.1:-84488375 | MS.gene070637:CDS |
| AAGTTTGGAGGAAGTGAAGA+TGG | 0.496634 | 7.1:-84488268 | MS.gene070637:CDS |
| TCCCTTTACTTCTAAGTCAC+CGG | 0.500276 | 7.1:+84488485 | None:intergenic |
| AGAAAGTGGAGAGATTAAGA+GGG | 0.503717 | 7.1:-84488406 | MS.gene070637:CDS |
| TCGAATGTAAGTCGAGAAAG+TGG | 0.504823 | 7.1:-84488420 | MS.gene070637:CDS |
| TGAGAATGAGCCAAGTTTGG+AGG | 0.510803 | 7.1:-84488280 | MS.gene070637:CDS |
| GGATGAAGTTGAAGCGGTGA+TGG | 0.511527 | 7.1:-84488385 | MS.gene070637:CDS |
| CTGCAATAAGATAAACAAGT+TGG | 0.540643 | 7.1:+84488646 | None:intergenic |
| TAAGAGGGATGAAGTTGAAG+CGG | 0.562793 | 7.1:-84488391 | MS.gene070637:CDS |
| ACTTGTTTATCTTATTGCAG+AGG | 0.590641 | 7.1:-84488643 | MS.gene070637:CDS |
| CGGAACGAAGTTGACAGAGA+AGG | 0.612537 | 7.1:+84488505 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | CATTGAATTTGTAAAAATTA+TGG | - | chr7.1:84488640-84488659 | MS.gene070637:CDS | 15.0% |
| !!! | AAAATGATCAAGAATTTTGA+TGG | - | chr7.1:84488593-84488612 | MS.gene070637:CDS | 20.0% |
| ! | GATGAACTTGAAGAAAAATA+TGG | - | chr7.1:84488398-84488417 | MS.gene070637:CDS | 25.0% |
| ! | TGAAGAAAAATATGGTTCAA+AGG | - | chr7.1:84488406-84488425 | MS.gene070637:CDS | 25.0% |
| !! | CAAAGAGTTATGTGTATTTT+GGG | - | chr7.1:84488536-84488555 | MS.gene070637:CDS | 25.0% |
| !!! | AATTTTGATGGAAATCAAGA+TGG | - | chr7.1:84488605-84488624 | MS.gene070637:CDS | 25.0% |
| !!! | TATGTGTATTTTGGGATTTA+AGG | - | chr7.1:84488544-84488563 | MS.gene070637:CDS | 25.0% |
| ACTTGTTTATCTTATTGCAG+AGG | - | chr7.1:84488085-84488104 | MS.gene070637:CDS | 30.0% | |
| CTGCAATAAGATAAACAAGT+TGG | + | chr7.1:84488085-84488104 | None:intergenic | 30.0% | |
| ! | GCAAAGAGTTATGTGTATTT+TGG | - | chr7.1:84488535-84488554 | MS.gene070637:CDS | 30.0% |
| ! | GTTTTTGATGAGAACAAAGA+TGG | - | chr7.1:84488491-84488510 | MS.gene070637:CDS | 30.0% |
| ! | TTAAGTTCTTCTCGAGTTTT+TGG | - | chr7.1:84488195-84488214 | MS.gene070637:CDS | 30.0% |
| !!! | TGTATTTTGGGATTTAAGGA+AGG | - | chr7.1:84488548-84488567 | MS.gene070637:CDS | 30.0% |
| AGAAAGTGGAGAGATTAAGA+GGG | - | chr7.1:84488322-84488341 | MS.gene070637:CDS | 35.0% | |
| GAAAGCAACTCAACTTTGTT+TGG | - | chr7.1:84488128-84488147 | MS.gene070637:CDS | 35.0% | |
| AAGTTTGGAGGAAGTGAAGA+TGG | - | chr7.1:84488460-84488479 | MS.gene070637:CDS | 40.0% | |
| ATCTTCACTTCCTCCAAACT+TGG | + | chr7.1:84488461-84488480 | None:intergenic | 40.0% | |
| GAGAAAGTGGAGAGATTAAG+AGG | - | chr7.1:84488321-84488340 | MS.gene070637:CDS | 40.0% | |
| TAAGAGGGATGAAGTTGAAG+CGG | - | chr7.1:84488337-84488356 | MS.gene070637:CDS | 40.0% | |
| TCCCTTTACTTCTAAGTCAC+CGG | + | chr7.1:84488246-84488265 | None:intergenic | 40.0% | |
| TCCGGTGACTTAGAAGTAAA+GGG | - | chr7.1:84488242-84488261 | MS.gene070637:CDS | 40.0% | |
| TCGAATGTAAGTCGAGAAAG+TGG | - | chr7.1:84488308-84488327 | MS.gene070637:CDS | 40.0% | |
| TGATGAGAATGAGCCAAGTT+TGG | - | chr7.1:84488445-84488464 | MS.gene070637:CDS | 40.0% | |
| TTCCGGTGACTTAGAAGTAA+AGG | - | chr7.1:84488241-84488260 | MS.gene070637:CDS | 40.0% | |
| CTTCTCTGTCAACTTCGTTC+CGG | - | chr7.1:84488224-84488243 | MS.gene070637:CDS | 45.0% | |
| TGAGAATGAGCCAAGTTTGG+AGG | - | chr7.1:84488448-84488467 | MS.gene070637:CDS | 45.0% | |
| CGGAACGAAGTTGACAGAGA+AGG | + | chr7.1:84488226-84488245 | None:intergenic | 50.0% | |
| GGATGAAGTTGAAGCGGTGA+TGG | - | chr7.1:84488343-84488362 | MS.gene070637:CDS | 50.0% | |
| ! | GAAGCGGTGATGGCGAAAAT+GGG | - | chr7.1:84488353-84488372 | MS.gene070637:CDS | 50.0% |
| ! | TGAAGCGGTGATGGCGAAAA+TGG | - | chr7.1:84488352-84488371 | MS.gene070637:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.1 | gene | 84488071 | 84488679 | 84488071 | ID=MS.gene070637 |
| chr7.1 | mRNA | 84488071 | 84488679 | 84488071 | ID=MS.gene070637.t1;Parent=MS.gene070637 |
| chr7.1 | exon | 84488071 | 84488679 | 84488071 | ID=MS.gene070637.t1.exon1;Parent=MS.gene070637.t1 |
| chr7.1 | CDS | 84488071 | 84488679 | 84488071 | ID=cds.MS.gene070637.t1;Parent=MS.gene070637.t1 |
| Gene Sequence |
| Protein sequence |
