AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene070795

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene070795.t1 MTR_4g050140 94.062 320 17 1 1 320 1 318 0.0 622
MS.gene070795.t1 MTR_7g107040 58.466 313 127 3 5 317 7 316 6.00e-128 368
MS.gene070795.t1 MTR_1g111280 38.226 327 180 10 4 317 25 342 2.09e-65 209
MS.gene070795.t1 MTR_3g012440 36.337 344 190 9 2 320 4 343 3.01e-64 206
MS.gene070795.t1 MTR_7g406880 35.168 327 192 9 11 319 27 351 1.98e-60 197
MS.gene070795.t1 MTR_7g056207 34.663 326 190 8 2 317 37 349 1.04e-54 182
MS.gene070795.t1 MTR_3g101420 33.333 315 189 7 13 319 15 316 7.12e-53 176
MS.gene070795.t1 MTR_4g086510 34.043 329 188 10 2 317 5 317 9.05e-53 176
MS.gene070795.t1 MTR_4g086480 33.634 333 187 9 2 318 5 319 2.76e-51 172
MS.gene070795.t1 MTR_1g105020 33.642 324 201 7 2 317 5 322 7.36e-51 171
MS.gene070795.t1 MTR_1g012430 35.202 321 190 11 4 319 2 309 1.61e-49 167
MS.gene070795.t1 MTR_6g065400 34.921 315 181 5 18 319 32 335 8.35e-49 166
MS.gene070795.t1 MTR_1g102170 37.121 264 155 4 40 300 68 323 9.22e-49 166
MS.gene070795.t1 MTR_1g082210 34.591 318 175 6 18 317 38 340 3.60e-48 165
MS.gene070795.t1 MTR_6g065270 32.710 321 186 6 17 319 29 337 4.33e-48 164
MS.gene070795.t1 MTR_6g065370 33.968 315 184 5 18 319 32 335 5.08e-48 164
MS.gene070795.t1 MTR_8g035520 34.641 306 191 6 18 319 26 326 2.19e-46 160
MS.gene070795.t1 MTR_6g065110 33.125 320 178 7 17 317 32 334 2.48e-46 160
MS.gene070795.t1 MTR_4g086470 35.577 312 187 7 12 319 18 319 2.69e-46 159
MS.gene070795.t1 MTR_1g089310 36.090 266 152 5 54 318 109 357 1.98e-44 155
MS.gene070795.t1 MTR_1g089310 34.615 286 157 5 47 317 70 340 1.99e-44 155
MS.gene070795.t1 MTR_5g005900 33.639 327 202 9 3 319 7 328 2.58e-44 154
MS.gene070795.t1 MTR_3g450680 32.121 330 205 9 1 319 12 333 2.87e-44 154
MS.gene070795.t1 MTR_7g093950 34.579 321 177 9 18 317 38 346 8.11e-44 154
MS.gene070795.t1 MTR_6g464510 33.684 285 175 6 38 317 51 326 2.72e-43 151
MS.gene070795.t1 MTR_6g065430 33.219 292 182 4 30 319 49 329 6.45e-43 150
MS.gene070795.t1 MTR_8g035540 34.397 282 177 5 39 317 40 316 1.06e-42 150
MS.gene070795.t1 MTR_6g065120 32.872 289 171 5 42 319 61 337 1.27e-42 150
MS.gene070795.t1 MTR_0007s0520 33.555 301 179 9 15 304 23 313 5.30e-42 148
MS.gene070795.t1 MTR_6g464280 33.750 320 184 10 12 317 19 324 1.21e-41 147
MS.gene070795.t1 MTR_1g102160 32.484 314 188 8 17 317 23 325 1.88e-41 147
MS.gene070795.t1 MTR_6g065340 33.101 287 156 6 42 319 71 330 2.84e-41 146
MS.gene070795.t1 MTR_5g005850 31.498 327 205 11 2 317 10 328 3.23e-41 146
MS.gene070795.t1 MTR_4g086540 31.056 322 184 7 2 317 15 304 3.36e-41 146
MS.gene070795.t1 MTR_6g464320 32.770 296 171 8 39 320 47 328 4.16e-41 146
MS.gene070795.t1 MTR_8g040860 29.503 322 213 7 5 319 16 330 2.24e-40 144
MS.gene070795.t1 MTR_5g005920 31.818 330 206 10 2 319 10 332 4.75e-40 143
MS.gene070795.t1 MTR_6g464120 37.255 204 119 3 39 240 51 247 2.59e-38 142
MS.gene070795.t1 MTR_8g035500 29.787 282 184 6 42 317 44 317 6.27e-37 134
MS.gene070795.t1 MTR_6g022710 31.579 266 146 4 66 300 158 418 2.67e-33 127
MS.gene070795.t1 MTR_7g051020 29.231 325 217 8 2 319 9 327 2.30e-32 122
MS.gene070795.t1 MTR_1g104933 30.793 328 203 9 1 319 6 318 2.57e-31 120
MS.gene070795.t1 MTR_1g104930 28.528 326 215 6 2 319 9 324 3.88e-30 117
MS.gene070795.t1 MTR_0007s0480 27.607 163 98 5 155 304 4 159 4.47e-11 61.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene070795.t1 AT5G06570 49.530 319 152 5 5 317 10 325 1.33e-104 309
MS.gene070795.t1 AT5G06570 49.530 319 152 5 5 317 10 325 1.33e-104 309
MS.gene070795.t1 AT5G06570 49.530 319 152 5 5 317 25 340 1.47e-104 310
MS.gene070795.t1 AT5G06570 49.530 319 152 5 5 317 25 340 1.47e-104 310
MS.gene070795.t1 AT5G16080 34.056 323 196 8 5 318 28 342 1.15e-56 187
MS.gene070795.t1 AT1G68620 35.494 324 187 11 5 320 23 332 2.69e-52 175
MS.gene070795.t1 AT5G27320 36.593 317 169 8 18 317 38 339 4.25e-52 175
MS.gene070795.t1 AT5G62180 36.624 314 174 8 18 317 21 323 8.08e-52 174
MS.gene070795.t1 AT1G47480 31.290 310 196 7 13 319 16 311 2.52e-48 164
MS.gene070795.t1 AT3G63010 34.675 323 173 7 18 319 38 343 3.44e-48 165
MS.gene070795.t1 AT3G05120 33.542 319 178 6 18 317 38 341 4.96e-48 164
MS.gene070795.t1 AT5G23530 34.202 307 184 6 18 319 35 328 2.44e-45 157
MS.gene070795.t1 AT2G45600 35.600 250 151 5 42 285 40 285 7.93e-45 155
MS.gene070795.t1 AT2G45610 32.028 281 182 4 42 318 49 324 7.65e-42 148
MS.gene070795.t1 AT3G48700 31.940 335 202 11 1 318 1 326 9.01e-41 145
MS.gene070795.t1 AT3G48690 32.416 327 206 8 1 318 1 321 3.66e-40 143
MS.gene070795.t1 AT1G19190 29.747 316 200 9 13 319 13 315 3.83e-39 140
MS.gene070795.t1 AT3G27320 32.975 279 152 5 55 300 120 396 3.08e-36 135
MS.gene070795.t1 AT3G27320 32.975 279 152 5 55 300 152 428 5.09e-36 135
MS.gene070795.t1 AT2G03550 28.182 330 208 9 1 319 1 312 1.91e-34 128
MS.gene070795.t1 AT5G14310 29.766 299 173 4 57 318 140 438 2.54e-34 130
MS.gene070795.t1 AT1G49650 31.861 317 190 12 13 318 69 370 3.50e-32 123
MS.gene070795.t1 AT1G49640 31.359 287 175 9 39 317 41 313 1.36e-31 120
MS.gene070795.t1 AT1G49660 30.034 293 173 10 1 278 1 276 8.97e-30 115

Find 74 sgRNAs with CRISPR-Local

Find 112 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCCTCAAGATTCTTACTTCT+TGG 0.205105 4.4:-52080550 None:intergenic
AGTTGGCACCGGTTCGGGTT+AGG 0.267807 4.4:+52079928 MS.gene070795:CDS
CATAATTTGGCGGTTCGGTT+TGG 0.283309 4.4:+52079891 MS.gene070795:CDS
TCTCCAACCACCACAAGAAT+TGG 0.284897 4.4:-52080586 None:intergenic
ATAATTTGGCGGTTCGGTTT+GGG 0.314873 4.4:+52079892 MS.gene070795:CDS
CAATTCATAGAGAAATATTA+AGG 0.314893 4.4:+52080766 MS.gene070795:CDS
GTTGGCACCGGTTCGGGTTA+GGG 0.314906 4.4:+52079929 MS.gene070795:CDS
GGTTGAGTTGGCACCGGTTC+GGG 0.335939 4.4:+52079923 MS.gene070795:CDS
AAAATGATTTGCTTAAAGAT+AGG 0.343018 4.4:+52080608 MS.gene070795:CDS
TGGTAATATAGCTCATAATT+TGG 0.349968 4.4:+52079878 MS.gene070795:CDS
GATGTTGAAGGCTGGGTTTA+AGG 0.350844 4.4:-52079460 None:intergenic
TTCTTACTTCTTGGTCCAAA+TGG 0.357264 4.4:-52080541 None:intergenic
CACCCACTTGTGAATCCATT+TGG 0.377391 4.4:+52080526 MS.gene070795:CDS
TTGGCGGTTCGGTTTGGGTT+TGG 0.380867 4.4:+52079897 MS.gene070795:CDS
CGGTTGAGTTGGCACCGGTT+CGG 0.381756 4.4:+52079922 MS.gene070795:CDS
ATCGACTTGCACCGGAGAAT+CGG 0.395410 4.4:+52079715 MS.gene070795:CDS
TGGAGGCTTTCTATACCAAT+TGG 0.407974 4.4:+52080490 MS.gene070795:CDS
TGCTATTGAAGATGGTTTCA+TGG 0.412164 4.4:+52079746 MS.gene070795:CDS
CTCCAACCACCACAAGAATT+GGG 0.412421 4.4:-52080585 None:intergenic
GGGCAAAGGATTATGCAAAA+AGG 0.422700 4.4:+52080629 MS.gene070795:CDS
TAGCTCATAATTTGGCGGTT+CGG 0.429884 4.4:+52079886 MS.gene070795:CDS
TGAACGTTGATGTTGAAGGC+TGG 0.430664 4.4:-52079468 None:intergenic
TTTATTTCGGGTGACTCGGC+TGG 0.436373 4.4:+52079855 MS.gene070795:CDS
TGGGTTTAAGGTTCGAACTA+TGG 0.437115 4.4:-52079448 None:intergenic
CTTCCATGTGCTATTGAAGA+TGG 0.442034 4.4:+52079738 MS.gene070795:CDS
TTGGCACCGGTTCGGGTTAG+GGG 0.442158 4.4:+52079930 MS.gene070795:CDS
TTGGAGGGTGATGAGTTAGC+TGG 0.444365 4.4:-52079564 None:intergenic
ATAGAGTATGTAGAATTTGA+AGG 0.444698 4.4:+52080673 MS.gene070795:CDS
CGGTCCTTCAGCTTCTGACT+TGG 0.447400 4.4:-52079994 None:intergenic
GGTTCGGTTTGGGTTTGGTT+CGG 0.452189 4.4:+52079902 MS.gene070795:CDS
CAATGTTGAGCAGGTATTGG+AGG 0.453576 4.4:+52080473 MS.gene070795:intron
GGCTTCAAGAAGAAGCTTTG+AGG 0.456117 4.4:+52079778 MS.gene070795:CDS
AGCTTCTGACTTGGTCCTCA+CGG 0.456270 4.4:-52079985 None:intergenic
ACATAACCCCTAACCCGAAC+CGG 0.456317 4.4:-52079936 None:intergenic
TGGGTTTGGTTCGGTTGAGT+TGG 0.466016 4.4:+52079911 MS.gene070795:CDS
TCCGGTGCAAGTCGATAGTC+AGG 0.479375 4.4:-52079708 None:intergenic
AAATGATTTGCTTAAAGATA+GGG 0.489878 4.4:+52080609 MS.gene070795:CDS
ATGAGTTAGCTGGCTTATAG+AGG 0.489986 4.4:-52079554 None:intergenic
CACGGTGGCGGCTTCTGCAT+CGG 0.491547 4.4:+52079612 MS.gene070795:CDS
GTCTTGAACGTTGATGTTGA+AGG 0.496577 4.4:-52079472 None:intergenic
AATTCATAGAGAAATATTAA+GGG 0.499926 4.4:+52080767 MS.gene070795:CDS
TCTGCATCGGCTCACGAACA+TGG 0.500777 4.4:+52079625 MS.gene070795:CDS
TCAACTTGGAACTTATTGAC+AGG 0.502436 4.4:+52080030 MS.gene070795:CDS
ATGGTTTCATGGCTGTTAAG+TGG 0.502959 4.4:+52079757 MS.gene070795:CDS
TGATCAGTATTCTCTCCAAT+TGG 0.505564 4.4:-52080505 None:intergenic
CCGTGAATGTAGTAAAAGAT+TGG 0.516981 4.4:-52079594 None:intergenic
GACCCAATTCTTGTGGTGGT+TGG 0.518707 4.4:+52080583 MS.gene070795:CDS
GCACATGGAAGCCGATTCTC+CGG 0.522531 4.4:-52079726 None:intergenic
GAACGTTGATGTTGAAGGCT+GGG 0.542067 4.4:-52079467 None:intergenic
CAAGTCAGAAGCTGAAGGAC+CGG 0.545544 4.4:+52079995 MS.gene070795:CDS
AAGTTGAGAAAAGCATGATC+CGG 0.562536 4.4:-52080014 None:intergenic
TGGTTCGGTTGAGTTGGCAC+CGG 0.564253 4.4:+52079917 MS.gene070795:CDS
GTTCTTCATGTCTATAACGA+CGG 0.568020 4.4:+52079423 MS.gene070795:CDS
ATTTCGGGTGACTCGGCTGG+TGG 0.569148 4.4:+52079858 MS.gene070795:CDS
GTGAGAATGAGATGGATCCT+TGG 0.570399 4.4:+52079802 MS.gene070795:CDS
GTCCAAATGGATTCACAAGT+GGG 0.578735 4.4:-52080528 None:intergenic
TCTTGACCCAATTCTTGTGG+TGG 0.585357 4.4:+52080579 MS.gene070795:CDS
ACCTGACTATCGACTTGCAC+CGG 0.588313 4.4:+52079707 MS.gene070795:CDS
TAGCTGGCTTATAGAGGCGA+AGG 0.596667 4.4:-52079548 None:intergenic
TAATATAGCTCATAATTTGG+CGG 0.598417 4.4:+52079881 MS.gene070795:CDS
GGTCCAAATGGATTCACAAG+TGG 0.601212 4.4:-52080529 None:intergenic
TTTGCTTAAAGATAGGGCAA+AGG 0.601510 4.4:+52080615 MS.gene070795:CDS
TGATCTTGACCCAATTCTTG+TGG 0.602002 4.4:+52080576 MS.gene070795:CDS
ACCATCAAAAGAGTTGATGT+TGG 0.606485 4.4:+52080735 MS.gene070795:CDS
CCCGGTCAAAATCAGCCACA+TGG 0.608763 4.4:-52079830 None:intergenic
AACATCAACGTTCAAGACAA+CGG 0.622066 4.4:+52079477 MS.gene070795:CDS
GAATTTGAAGGACAACAACA+TGG 0.622261 4.4:+52080685 MS.gene070795:CDS
TTTGAGGAGTGAGAATGAGA+TGG 0.629316 4.4:+52079794 MS.gene070795:CDS
ACCAAGAAGTAAGAATCTTG+AGG 0.633825 4.4:+52080549 MS.gene070795:CDS
AAACCATCTTCAATAGCACA+TGG 0.641464 4.4:-52079741 None:intergenic
AGGACCAAGTCAGAAGCTGA+AGG 0.644457 4.4:+52079990 MS.gene070795:CDS
ACTATAGTTGACGAATGTCG+TGG 0.650039 4.4:+52079399 MS.gene070795:CDS
TCAGCCACATGGCTCAACCA+AGG 0.660011 4.4:-52079819 None:intergenic
GGATCCTTGGTTGAGCCATG+TGG 0.675420 4.4:+52079815 MS.gene070795:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TATAAAAAGTGTCAAAATTA+TGG + chr4.4:52080379-52080398 MS.gene070795:intron 15.0%
!! AATTAAATCATTATCAGTGT+CGG - chr4.4:52080282-52080301 None:intergenic 20.0%
!! CGTGCATAATATAAAATATT+TGG - chr4.4:52080091-52080110 None:intergenic 20.0%
!!! AAAATGATTTGCTTAAAGAT+AGG + chr4.4:52080608-52080627 MS.gene070795:CDS 20.0%
!!! AAATGATTTGCTTAAAGATA+GGG + chr4.4:52080609-52080628 MS.gene070795:CDS 20.0%
! AATTAGACATAATCATGTGT+CGG + chr4.4:52080218-52080237 MS.gene070795:intron 25.0%
! ATAGAGTATGTAGAATTTGA+AGG + chr4.4:52080673-52080692 MS.gene070795:CDS 25.0%
! ATTAAATCATTATCAGTGTC+GGG - chr4.4:52080281-52080300 None:intergenic 25.0%
! TAATATAGCTCATAATTTGG+CGG + chr4.4:52079881-52079900 MS.gene070795:CDS 25.0%
! TAATTGTTTAGTACAATGTC+CGG - chr4.4:52080183-52080202 None:intergenic 25.0%
! TGGTAATATAGCTCATAATT+TGG + chr4.4:52079878-52079897 MS.gene070795:CDS 25.0%
!! AGTATTTGTTGCTTATTGAA+AGG + chr4.4:52080430-52080449 MS.gene070795:intron 25.0%
!!! CACTTTTTATATTGTTCTCA+CGG - chr4.4:52080370-52080389 None:intergenic 25.0%
ATGCAAAAAGGCTTAAGAAT+TGG + chr4.4:52080641-52080660 MS.gene070795:CDS 30.0%
TGAGAAAAGAGTTACAAACT+CGG + chr4.4:52080148-52080167 MS.gene070795:intron 30.0%
TGCAAAAAGGCTTAAGAATT+GGG + chr4.4:52080642-52080661 MS.gene070795:CDS 30.0%
! CCAATCTTTTACTACATTCA+CGG + chr4.4:52079594-52079613 MS.gene070795:CDS 30.0%
!! GTAAAAGATTGGTAGCTTTT+TGG - chr4.4:52079586-52079605 None:intergenic 30.0%
!! TTTTGACCGGGTTTTTATTT+CGG + chr4.4:52079842-52079861 MS.gene070795:CDS 30.0%
!!! TCTTTTGATGGTTCTGAATT+TGG - chr4.4:52080727-52080746 None:intergenic 30.0%
AAACCATCTTCAATAGCACA+TGG - chr4.4:52079744-52079763 None:intergenic 35.0%
AACATCAACGTTCAAGACAA+CGG + chr4.4:52079477-52079496 MS.gene070795:CDS 35.0%
ACCAAGAAGTAAGAATCTTG+AGG + chr4.4:52080549-52080568 MS.gene070795:CDS 35.0%
CCGTGAATGTAGTAAAAGAT+TGG - chr4.4:52079597-52079616 None:intergenic 35.0%
GAATTTGAAGGACAACAACA+TGG + chr4.4:52080685-52080704 MS.gene070795:CDS 35.0%
GCAAAAAGGCTTAAGAATTG+GGG + chr4.4:52080643-52080662 MS.gene070795:CDS 35.0%
GTTCTTCATGTCTATAACGA+CGG + chr4.4:52079423-52079442 MS.gene070795:CDS 35.0%
TCAACTTGGAACTTATTGAC+AGG + chr4.4:52080030-52080049 MS.gene070795:CDS 35.0%
TCCTCAAGATTCTTACTTCT+TGG - chr4.4:52080553-52080572 None:intergenic 35.0%
TGATCAGTATTCTCTCCAAT+TGG - chr4.4:52080508-52080527 None:intergenic 35.0%
TGCTATTGAAGATGGTTTCA+TGG + chr4.4:52079746-52079765 MS.gene070795:CDS 35.0%
TTCTTACTTCTTGGTCCAAA+TGG - chr4.4:52080544-52080563 None:intergenic 35.0%
TTGGTATGCAAAGTGAAGAT+AGG - chr4.4:52080072-52080091 None:intergenic 35.0%
TTTGCTTAAAGATAGGGCAA+AGG + chr4.4:52080615-52080634 MS.gene070795:CDS 35.0%
! AAGTTGAGAAAAGCATGATC+CGG - chr4.4:52080017-52080036 None:intergenic 35.0%
! ACCAACATCAACTCTTTTGA+TGG - chr4.4:52080739-52080758 None:intergenic 35.0%
! ATCTTTTACTACATTCACGG+TGG + chr4.4:52079597-52079616 MS.gene070795:CDS 35.0%
!! AAAGATTGGTAGCTTTTTGG+AGG - chr4.4:52079583-52079602 None:intergenic 35.0%
!! AAGATTGGTAGCTTTTTGGA+GGG - chr4.4:52079582-52079601 None:intergenic 35.0%
!! ACCATCAAAAGAGTTGATGT+TGG + chr4.4:52080735-52080754 MS.gene070795:CDS 35.0%
!! TTTGACCGGGTTTTTATTTC+GGG + chr4.4:52079843-52079862 MS.gene070795:CDS 35.0%
!!! AAGCAGTAGTTTTGACAGTT+AGG - chr4.4:52079651-52079670 None:intergenic 35.0%
ACTATAGTTGACGAATGTCG+TGG + chr4.4:52079399-52079418 MS.gene070795:CDS 40.0%
AGTCACCCGAAATAAAAACC+CGG - chr4.4:52079851-52079870 None:intergenic 40.0%
ATGGTTTCATGGCTGTTAAG+TGG + chr4.4:52079757-52079776 MS.gene070795:CDS 40.0%
CAATACCTGCTCAACATTGA+AGG - chr4.4:52080472-52080491 None:intergenic 40.0%
CTTCAATGTTGAGCAGGTAT+TGG + chr4.4:52080470-52080489 MS.gene070795:intron 40.0%
GGGCAAAGGATTATGCAAAA+AGG + chr4.4:52080629-52080648 MS.gene070795:CDS 40.0%
GTCTTGAACGTTGATGTTGA+AGG - chr4.4:52079475-52079494 None:intergenic 40.0%
TAGCTCATAATTTGGCGGTT+CGG + chr4.4:52079886-52079905 MS.gene070795:CDS 40.0%
TGATCTTGACCCAATTCTTG+TGG + chr4.4:52080576-52080595 MS.gene070795:CDS 40.0%
TGGAGGCTTTCTATACCAAT+TGG + chr4.4:52080490-52080509 MS.gene070795:CDS 40.0%
TTGTACCTTCAATGTTGAGC+AGG + chr4.4:52080464-52080483 MS.gene070795:intron 40.0%
TTTGAGGAGTGAGAATGAGA+TGG + chr4.4:52079794-52079813 MS.gene070795:CDS 40.0%
! AAGACAACGGCACTGTTTTA+TGG + chr4.4:52079490-52079509 MS.gene070795:CDS 40.0%
! ATGAGTTAGCTGGCTTATAG+AGG - chr4.4:52079557-52079576 None:intergenic 40.0%
! CTTCCATGTGCTATTGAAGA+TGG + chr4.4:52079738-52079757 MS.gene070795:CDS 40.0%
! GGATCATGCTTTTCTCAACT+TGG + chr4.4:52080016-52080035 MS.gene070795:CDS 40.0%
! GGTTAGGGGTTATGTTTTGT+TGG + chr4.4:52079944-52079963 MS.gene070795:CDS 40.0%
! GTCCAAATGGATTCACAAGT+GGG - chr4.4:52080531-52080550 None:intergenic 40.0%
! TGGGTTTAAGGTTCGAACTA+TGG - chr4.4:52079451-52079470 None:intergenic 40.0%
! TTTTACTACATTCACGGTGG+CGG + chr4.4:52079600-52079619 MS.gene070795:CDS 40.0%
!! ATAATTTGGCGGTTCGGTTT+GGG + chr4.4:52079892-52079911 MS.gene070795:CDS 40.0%
!! GGTTTTTATTTCGGGTGACT+CGG + chr4.4:52079851-52079870 MS.gene070795:CDS 40.0%
!! GTTTTGTTGGCACCGTTTTT+TGG + chr4.4:52079957-52079976 MS.gene070795:CDS 40.0%
CACCCACTTGTGAATCCATT+TGG + chr4.4:52080526-52080545 MS.gene070795:CDS 45.0%
CTCACGGTTCCACCAAAAAA+CGG - chr4.4:52079972-52079991 None:intergenic 45.0%
CTCCAACCACCACAAGAATT+GGG - chr4.4:52080588-52080607 None:intergenic 45.0%
GAACGTTGATGTTGAAGGCT+GGG - chr4.4:52079470-52079489 None:intergenic 45.0%
GATGTTGAAGGCTGGGTTTA+AGG - chr4.4:52079463-52079482 None:intergenic 45.0%
GGCTTCAAGAAGAAGCTTTG+AGG + chr4.4:52079778-52079797 MS.gene070795:CDS 45.0%
GTGAGAATGAGATGGATCCT+TGG + chr4.4:52079802-52079821 MS.gene070795:CDS 45.0%
TCTCCAACCACCACAAGAAT+TGG - chr4.4:52080589-52080608 None:intergenic 45.0%
TCTTGACCCAATTCTTGTGG+TGG + chr4.4:52080579-52080598 MS.gene070795:CDS 45.0%
TGAACGTTGATGTTGAAGGC+TGG - chr4.4:52079471-52079490 None:intergenic 45.0%
! GGTCCAAATGGATTCACAAG+TGG - chr4.4:52080532-52080551 None:intergenic 45.0%
!! CAATGTTGAGCAGGTATTGG+AGG + chr4.4:52080473-52080492 MS.gene070795:intron 45.0%
!! CATAATTTGGCGGTTCGGTT+TGG + chr4.4:52079891-52079910 MS.gene070795:CDS 45.0%
!!! TTGTTGGCACCGTTTTTTGG+TGG + chr4.4:52079960-52079979 MS.gene070795:CDS 45.0%
ACAAACTCGGTCACAGACAC+CGG + chr4.4:52080161-52080180 MS.gene070795:intron 50.0%
ACATAACCCCTAACCCGAAC+CGG - chr4.4:52079939-52079958 None:intergenic 50.0%
AGGACCAAGTCAGAAGCTGA+AGG + chr4.4:52079990-52080009 MS.gene070795:CDS 50.0%
CAAGTCAGAAGCTGAAGGAC+CGG + chr4.4:52079995-52080014 MS.gene070795:CDS 50.0%
GACCCAATTCTTGTGGTGGT+TGG + chr4.4:52080583-52080602 MS.gene070795:CDS 50.0%
TTGGAGGGTGATGAGTTAGC+TGG - chr4.4:52079567-52079586 None:intergenic 50.0%
! GTGTCGGGTATGACTTGTCT+CGG - chr4.4:52080266-52080285 None:intergenic 50.0%
! TAGCTGGCTTATAGAGGCGA+AGG - chr4.4:52079551-52079570 None:intergenic 50.0%
! TTTATTTCGGGTGACTCGGC+TGG + chr4.4:52079855-52079874 MS.gene070795:CDS 50.0%
!! ACCTGACTATCGACTTGCAC+CGG + chr4.4:52079707-52079726 MS.gene070795:CDS 50.0%
!! AGCTTCTGACTTGGTCCTCA+CGG - chr4.4:52079988-52080007 None:intergenic 50.0%
!! ATCGACTTGCACCGGAGAAT+CGG + chr4.4:52079715-52079734 MS.gene070795:CDS 50.0%
!! CCATGTGGCTGATTTTGACC+GGG + chr4.4:52079830-52079849 MS.gene070795:CDS 50.0%
!! GCCATGTGGCTGATTTTGAC+CGG + chr4.4:52079829-52079848 MS.gene070795:CDS 50.0%
!! GGTTCGGTTTGGGTTTGGTT+CGG + chr4.4:52079902-52079921 MS.gene070795:CDS 50.0%
!! TGGGTTTGGTTCGGTTGAGT+TGG + chr4.4:52079911-52079930 MS.gene070795:CDS 50.0%
!!! CGTTTTTTGGTGGAACCGTG+AGG + chr4.4:52079970-52079989 MS.gene070795:CDS 50.0%
CCCGGTCAAAATCAGCCACA+TGG - chr4.4:52079833-52079852 None:intergenic 55.0%
CGGTCCTTCAGCTTCTGACT+TGG - chr4.4:52079997-52080016 None:intergenic 55.0%
GGATCCTTGGTTGAGCCATG+TGG + chr4.4:52079815-52079834 MS.gene070795:CDS 55.0%
TCAGCCACATGGCTCAACCA+AGG - chr4.4:52079822-52079841 None:intergenic 55.0%
TCCGGTGCAAGTCGATAGTC+AGG - chr4.4:52079711-52079730 None:intergenic 55.0%
! GCACATGGAAGCCGATTCTC+CGG - chr4.4:52079729-52079748 None:intergenic 55.0%
!! TCTGCATCGGCTCACGAACA+TGG + chr4.4:52079625-52079644 MS.gene070795:CDS 55.0%
!! TGGTTCGGTTGAGTTGGCAC+CGG + chr4.4:52079917-52079936 MS.gene070795:CDS 55.0%
!! TTGGCGGTTCGGTTTGGGTT+TGG + chr4.4:52079897-52079916 MS.gene070795:CDS 55.0%
! ATTTCGGGTGACTCGGCTGG+TGG + chr4.4:52079858-52079877 MS.gene070795:CDS 60.0%
!! AGTTGGCACCGGTTCGGGTT+AGG + chr4.4:52079928-52079947 MS.gene070795:CDS 60.0%
!! CGGTTGAGTTGGCACCGGTT+CGG + chr4.4:52079922-52079941 MS.gene070795:CDS 60.0%
!! GGTTGAGTTGGCACCGGTTC+GGG + chr4.4:52079923-52079942 MS.gene070795:CDS 60.0%
!! GTTGGCACCGGTTCGGGTTA+GGG + chr4.4:52079929-52079948 MS.gene070795:CDS 60.0%
!! TTGGCACCGGTTCGGGTTAG+GGG + chr4.4:52079930-52079949 MS.gene070795:CDS 60.0%
! CACGGTGGCGGCTTCTGCAT+CGG + chr4.4:52079612-52079631 MS.gene070795:CDS 65.0%


Chromosome Type Strat End Strand Name
chr4.4 gene 52079390 52080786 52079390 ID=MS.gene070795
chr4.4 mRNA 52079390 52080786 52079390 ID=MS.gene070795.t1;Parent=MS.gene070795
chr4.4 exon 52079390 52080051 52079390 ID=MS.gene070795.t1.exon1;Parent=MS.gene070795.t1
chr4.4 CDS 52079390 52080051 52079390 ID=cds.MS.gene070795.t1;Parent=MS.gene070795.t1
chr4.4 exon 52080486 52080786 52080486 ID=MS.gene070795.t1.exon2;Parent=MS.gene070795.t1
chr4.4 CDS 52080486 52080786 52080486 ID=cds.MS.gene070795.t1;Parent=MS.gene070795.t1
Gene Sequence

>MS.gene070795

ATGTCAAAAACTATAGTTGACGAATGTCGTGGTGTTCTTCATGTCTATAACGACGGTTCCATAGTTCGAACCTTAAACCCAGCCTTCAACATCAACGTTCAAGACAACGGCACTGTTTTATGGAAAGATGTTGTTTTCGACTCAACACGCAATCTTCACCTTCGCCTCTATAAGCCAGCTAACTCATCACCCTCCAAAAAGCTACCAATCTTTTACTACATTCACGGTGGCGGCTTCTGCATCGGCTCACGAACATGGCCTAACTGTCAAAACTACTGCTTCAAGCTCGCTTCTGAGCTTGAAGCAGTAGTTGTTGCACCTGACTATCGACTTGCACCGGAGAATCGGCTTCCATGTGCTATTGAAGATGGTTTCATGGCTGTTAAGTGGCTTCAAGAAGAAGCTTTGAGGAGTGAGAATGAGATGGATCCTTGGTTGAGCCATGTGGCTGATTTTGACCGGGTTTTTATTTCGGGTGACTCGGCTGGTGGTAATATAGCTCATAATTTGGCGGTTCGGTTTGGGTTTGGTTCGGTTGAGTTGGCACCGGTTCGGGTTAGGGGTTATGTTTTGTTGGCACCGTTTTTTGGTGGAACCGTGAGGACCAAGTCAGAAGCTGAAGGACCGGATCATGCTTTTCTCAACTTGGAACTTATTGACAGGTTCTCAAATTTGATTTCCTATCTTCACTTTGCATACCAAATATTTTATATTATGCACGCAAAATCATCTATTAATATTCTATAATAATTTAGTTATGAGAAAAGAGTTACAAACTCGGTCACAGACACCGGACATTGTACTAAACAATTACAAATAAAATGATATAATTAGACATAATCATGTGTCGGTGTCGTGTTTACATATCTGACACCGAGACAAGTCATACCCGACACTGATAATGATTTAATTTTTTGATGTGATTGAATCAAATCATGTGCATTAGTATTGAACGATATGTGTCAGATATCAACACGCCGTGAGAACAATATAAAAAGTGTCAAAATTATGGTGTGTCTAGAATAGAACATTGATTTGTTAGTATTTGTTGCTTATTGAAAGGTGTATTTGTTTTTGTACCTTCAATGTTGAGCAGGTATTGGAGGCTTTCTATACCAATTGGAGAGAATACTGATCACCCACTTGTGAATCCATTTGGACCAAGAAGTAAGAATCTTGAGGAAGTTGATCTTGACCCAATTCTTGTGGTGGTTGGAGAAAATGATTTGCTTAAAGATAGGGCAAAGGATTATGCAAAAAGGCTTAAGAATTGGGGTAAAAATATAGAGTATGTAGAATTTGAAGGACAACAACATGGATTCTTTACTATTGATCCAAATTCAGAACCATCAAAAGAGTTGATGTTGGTCATCAAGCAATTCATAGAGAAATATTAA
Protein sequence

>MS.gene070795.t1

MSKTIVDECRGVLHVYNDGSIVRTLNPAFNINVQDNGTVLWKDVVFDSTRNLHLRLYKPANSSPSKKLPIFYYIHGGGFCIGSRTWPNCQNYCFKLASELEAVVVAPDYRLAPENRLPCAIEDGFMAVKWLQEEALRSENEMDPWLSHVADFDRVFISGDSAGGNIAHNLAVRFGFGSVELAPVRVRGYVLLAPFFGGTVRTKSEAEGPDHAFLNLELIDRYWRLSIPIGENTDHPLVNPFGPRSKNLEEVDLDPILVVVGENDLLKDRAKDYAKRLKNWGKNIEYVEFEGQQHGFFTIDPNSEPSKELMLVIKQFIEKY