Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene070796.t1 | XP_013455692.1 | 96 | 50 | 2 | 0 | 101 | 150 | 103 | 152 | 5.30E-18 | 100.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene070796.t1 | A0A072UKU9 | 96.0 | 50 | 2 | 0 | 101 | 150 | 103 | 152 | 3.8e-18 | 100.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene05548 | MS.gene070796 | 0.80866 | 2.78E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene070796.t1 | MTR_4g050130 | 86.486 | 148 | 19 | 1 | 3 | 150 | 6 | 152 | 1.74e-56 | 174 |
| MS.gene070796.t1 | MTR_2g087830 | 38.971 | 136 | 65 | 5 | 15 | 149 | 8 | 126 | 9.66e-26 | 95.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene070796.t1 | AT3G11690 | 37.888 | 161 | 79 | 5 | 10 | 149 | 41 | 201 | 6.27e-17 | 75.1 |
| MS.gene070796.t1 | AT5G06380 | 34.752 | 141 | 77 | 3 | 10 | 149 | 12 | 138 | 1.38e-15 | 69.3 |
| MS.gene070796.t1 | AT2G27180 | 41.176 | 136 | 68 | 2 | 17 | 149 | 33 | 159 | 2.18e-13 | 64.3 |
Find 31 sgRNAs with CRISPR-Local
Find 40 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGTTTGCGGGAACCGGGTTT+TGG | 0.261947 | 4.4:-52083426 | MS.gene070796:CDS |
| TGGGCACGGTTTGCGGGAAC+CGG | 0.294255 | 4.4:-52083433 | MS.gene070796:CDS |
| GAGATGTGGGCACGGTTTGC+GGG | 0.342993 | 4.4:-52083439 | MS.gene070796:CDS |
| CAGAGATCAAGCAGTGTTGC+TGG | 0.393742 | 4.4:-52083550 | MS.gene070796:CDS |
| GGAGATGTGGGCACGGTTTG+CGG | 0.441228 | 4.4:-52083440 | MS.gene070796:CDS |
| GGGCACGGTTTGCGGGAACC+GGG | 0.458010 | 4.4:-52083432 | MS.gene070796:CDS |
| TGATATTAAGATTGATTCGT+TGG | 0.462200 | 4.4:-52083524 | MS.gene070796:CDS |
| CGTGAAGGATCGTGCGGCGG+AGG | 0.484879 | 4.4:-52083491 | MS.gene070796:CDS |
| CGCGGCGTTGGCGAAACAGC+AGG | 0.485789 | 4.4:-52083775 | MS.gene070796:CDS |
| TGAATGAGTGAGAGCGTGGC+TGG | 0.527453 | 4.4:+52083822 | None:intergenic |
| TTTGAAAAGTGCGGTCGGGA+CGG | 0.539525 | 4.4:+52083672 | None:intergenic |
| AAAATCGGAGGAGGTTGCGT+TGG | 0.541827 | 4.4:-52083467 | MS.gene070796:CDS |
| CGTGGTAAAAGACGCGGCGT+TGG | 0.551905 | 4.4:-52083787 | MS.gene070796:CDS |
| GGAGGAGGTTGCGTTGGAGA+AGG | 0.558865 | 4.4:-52083461 | MS.gene070796:CDS |
| GCATGGACAGCAACAGCATA+CGG | 0.560127 | 4.4:+52083729 | None:intergenic |
| CCTACGTCGTCTTCGATGAA+CGG | 0.563027 | 4.4:+52083645 | None:intergenic |
| AGAGATCAAGCAGTGTTGCT+GGG | 0.568754 | 4.4:-52083549 | MS.gene070796:CDS |
| TGCGTTGGAGAAGGAGATGT+GGG | 0.586252 | 4.4:-52083452 | MS.gene070796:CDS |
| ACGACGTTGAGAACCGTGCA+TGG | 0.594359 | 4.4:+52083712 | None:intergenic |
| ATGCTGTTGCTGTCCATGCA+CGG | 0.601793 | 4.4:-52083725 | MS.gene070796:CDS |
| AGAATCACTTCTCCAAAACC+CGG | 0.610774 | 4.4:+52083414 | None:intergenic |
| GTTGGAAGAGCATTTCGTGA+AGG | 0.624024 | 4.4:-52083506 | MS.gene070796:CDS |
| TTGCGTTGGAGAAGGAGATG+TGG | 0.625295 | 4.4:-52083453 | MS.gene070796:CDS |
| TGGAGAAGGAGATGTGGGCA+CGG | 0.638141 | 4.4:-52083447 | MS.gene070796:CDS |
| TTTCGTGAAGGATCGTGCGG+CGG | 0.669525 | 4.4:-52083494 | MS.gene070796:CDS |
| CATTCACGTCCAACGCCGCG+TGG | 0.684079 | 4.4:-52083805 | MS.gene070796:CDS |
| GACGTGAATGAGTGAGAGCG+TGG | 0.688394 | 4.4:+52083818 | None:intergenic |
| CCGTTCATCGAAGACGACGT+AGG | 0.689307 | 4.4:-52083645 | MS.gene070796:CDS |
| GCATTTCGTGAAGGATCGTG+CGG | 0.703359 | 4.4:-52083497 | MS.gene070796:CDS |
| ACGCCGCGTGGTAAAAGACG+CGG | 0.704364 | 4.4:-52083793 | MS.gene070796:CDS |
| TCGGGACGGAGTAGACTGCG+AGG | 0.785457 | 4.4:+52083686 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TGATATTAAGATTGATTCGT+TGG | - | chr4.4:52083704-52083723 | MS.gene070796:CDS | 25.0% |
| !!! | AACGGCTTTTTTGAAAAGTG+CGG | + | chr4.4:52083568-52083587 | None:intergenic | 35.0% |
| AGAATCACTTCTCCAAAACC+CGG | + | chr4.4:52083817-52083836 | None:intergenic | 40.0% | |
| GAAAAACAACGTTAACGACG+TGG | - | chr4.4:52083641-52083660 | MS.gene070796:CDS | 40.0% | |
| !!! | CTTTTTTGAAAAGTGCGGTC+GGG | + | chr4.4:52083563-52083582 | None:intergenic | 40.0% |
| !!! | GCTTTTTTGAAAAGTGCGGT+CGG | + | chr4.4:52083564-52083583 | None:intergenic | 40.0% |
| ! | AGAGATCAAGCAGTGTTGCT+GGG | - | chr4.4:52083679-52083698 | MS.gene070796:CDS | 45.0% |
| !! | GTTGGAAGAGCATTTCGTGA+AGG | - | chr4.4:52083722-52083741 | MS.gene070796:CDS | 45.0% |
| AAAATCGGAGGAGGTTGCGT+TGG | - | chr4.4:52083761-52083780 | MS.gene070796:CDS | 50.0% | |
| ATGCTGTTGCTGTCCATGCA+CGG | - | chr4.4:52083503-52083522 | MS.gene070796:CDS | 50.0% | |
| CAGAGATCAAGCAGTGTTGC+TGG | - | chr4.4:52083678-52083697 | MS.gene070796:CDS | 50.0% | |
| CCTACGTCGTCTTCGATGAA+CGG | + | chr4.4:52083586-52083605 | None:intergenic | 50.0% | |
| GCATGGACAGCAACAGCATA+CGG | + | chr4.4:52083502-52083521 | None:intergenic | 50.0% | |
| GCATTTCGTGAAGGATCGTG+CGG | - | chr4.4:52083731-52083750 | MS.gene070796:CDS | 50.0% | |
| TGCGTTGGAGAAGGAGATGT+GGG | - | chr4.4:52083776-52083795 | MS.gene070796:CDS | 50.0% | |
| TTGCGTTGGAGAAGGAGATG+TGG | - | chr4.4:52083775-52083794 | MS.gene070796:CDS | 50.0% | |
| ! | TTTGAAAAGTGCGGTCGGGA+CGG | + | chr4.4:52083559-52083578 | None:intergenic | 50.0% |
| ACGACGTTGAGAACCGTGCA+TGG | + | chr4.4:52083519-52083538 | None:intergenic | 55.0% | |
| CCGTTCATCGAAGACGACGT+AGG | - | chr4.4:52083583-52083602 | MS.gene070796:CDS | 55.0% | |
| GACGTGAATGAGTGAGAGCG+TGG | + | chr4.4:52083413-52083432 | None:intergenic | 55.0% | |
| TCGTGCGGCGGAGGAAAAAT+CGG | - | chr4.4:52083746-52083765 | MS.gene070796:CDS | 55.0% | |
| TGGAGAAGGAGATGTGGGCA+CGG | - | chr4.4:52083781-52083800 | MS.gene070796:CDS | 55.0% | |
| TTTCGTGAAGGATCGTGCGG+CGG | - | chr4.4:52083734-52083753 | MS.gene070796:CDS | 55.0% | |
| ! | TGAATGAGTGAGAGCGTGGC+TGG | + | chr4.4:52083409-52083428 | None:intergenic | 55.0% |
| ACGCCGCGTGGTAAAAGACG+CGG | - | chr4.4:52083435-52083454 | MS.gene070796:CDS | 60.0% | |
| CGTGGTAAAAGACGCGGCGT+TGG | - | chr4.4:52083441-52083460 | MS.gene070796:CDS | 60.0% | |
| GAGATGTGGGCACGGTTTGC+GGG | - | chr4.4:52083789-52083808 | MS.gene070796:CDS | 60.0% | |
| GGAGATGTGGGCACGGTTTG+CGG | - | chr4.4:52083788-52083807 | MS.gene070796:CDS | 60.0% | |
| GGAGGAGGTTGCGTTGGAGA+AGG | - | chr4.4:52083767-52083786 | MS.gene070796:CDS | 60.0% | |
| GGCGGAGGAAAAATCGGAGG+AGG | - | chr4.4:52083752-52083771 | MS.gene070796:CDS | 60.0% | |
| TGCGGCGGAGGAAAAATCGG+AGG | - | chr4.4:52083749-52083768 | MS.gene070796:CDS | 60.0% | |
| ! | ACGCCGCGTCTTTTACCACG+CGG | + | chr4.4:52083441-52083460 | None:intergenic | 60.0% |
| ! | CGTCTTTTACCACGCGGCGT+TGG | + | chr4.4:52083435-52083454 | None:intergenic | 60.0% |
| ! | GGTTTGCGGGAACCGGGTTT+TGG | - | chr4.4:52083802-52083821 | MS.gene070796:CDS | 60.0% |
| CATTCACGTCCAACGCCGCG+TGG | - | chr4.4:52083423-52083442 | MS.gene070796:CDS | 65.0% | |
| TCGGGACGGAGTAGACTGCG+AGG | + | chr4.4:52083545-52083564 | None:intergenic | 65.0% | |
| TGGGCACGGTTTGCGGGAAC+CGG | - | chr4.4:52083795-52083814 | MS.gene070796:CDS | 65.0% | |
| CGTGAAGGATCGTGCGGCGG+AGG | - | chr4.4:52083737-52083756 | MS.gene070796:CDS | 70.0% | |
| GGGCACGGTTTGCGGGAACC+GGG | - | chr4.4:52083796-52083815 | MS.gene070796:CDS | 70.0% | |
| !! | CGCGGCGTTGGCGAAACAGC+AGG | - | chr4.4:52083453-52083472 | MS.gene070796:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.4 | gene | 52083399 | 52083851 | 52083399 | ID=MS.gene070796 |
| chr4.4 | mRNA | 52083399 | 52083851 | 52083399 | ID=MS.gene070796.t1;Parent=MS.gene070796 |
| chr4.4 | exon | 52083399 | 52083851 | 52083399 | ID=MS.gene070796.t1.exon1;Parent=MS.gene070796.t1 |
| chr4.4 | CDS | 52083399 | 52083851 | 52083399 | ID=cds.MS.gene070796.t1;Parent=MS.gene070796.t1 |
| Gene Sequence |
| Protein sequence |
