Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene071005.t1 | XP_003604116.1 | 97 | 135 | 4 | 0 | 1 | 135 | 68 | 202 | 2.10E-72 | 281.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene071005.t1 | G7JUF2 | 97.0 | 135 | 4 | 0 | 1 | 135 | 68 | 202 | 1.5e-72 | 281.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene057684 | MS.gene071005 | 0.801972 | 7.00E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 35 sgRNAs with CRISPR-Local
Find 96 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AACCTTAGGTTTGCAGGATT+TGG | 0.251778 | 4.3:-89705505 | None:intergenic |
| ATCAGTAACCTTAGGTTTGC+AGG | 0.256518 | 4.3:-89705511 | None:intergenic |
| ATCTAAATGAGAATGCTAAA+TGG | 0.314684 | 4.3:+89705439 | MS.gene071005:CDS |
| AAAGGTAATACATCCGGTTA+TGG | 0.343863 | 4.3:+89706852 | MS.gene071005:CDS |
| GACCGTGTTCAATAATATTT+CGG | 0.348371 | 4.3:+89706621 | MS.gene071005:CDS |
| CTAGAGAATACGTGCTAACT+TGG | 0.350444 | 4.3:+89706788 | MS.gene071005:CDS |
| AAGGTAATACATCCGGTTAT+GGG | 0.400139 | 4.3:+89706853 | MS.gene071005:CDS |
| TCTAAATGAGAATGCTAAAT+GGG | 0.425962 | 4.3:+89705440 | MS.gene071005:CDS |
| TTGGAAATTGTGGGAGGGGA+AGG | 0.429664 | 4.3:+89706807 | MS.gene071005:CDS |
| ACGTGCTAACTTGGAAATTG+TGG | 0.456235 | 4.3:+89706797 | MS.gene071005:CDS |
| TATCAATGCAACCCCATAAC+CGG | 0.457506 | 4.3:-89706865 | None:intergenic |
| CAAGTTAGCACGTATTCTCT+AGG | 0.463689 | 4.3:-89706787 | None:intergenic |
| AAATCTGATGGTTCTGAAGT+TGG | 0.471935 | 4.3:+89706733 | MS.gene071005:CDS |
| TATTCTCTAGGTTTCAAAAG+AGG | 0.479731 | 4.3:-89706775 | None:intergenic |
| CGTGCTAACTTGGAAATTGT+GGG | 0.500606 | 4.3:+89706798 | MS.gene071005:CDS |
| CAATGACTACAGAAAACAAT+GGG | 0.504178 | 4.3:+89706672 | MS.gene071005:CDS |
| TAACTTGGAAATTGTGGGAG+GGG | 0.526844 | 4.3:+89706803 | MS.gene071005:CDS |
| TTATATAAAGGTAATACATC+CGG | 0.529251 | 4.3:+89706846 | MS.gene071005:CDS |
| ATTCTCTAGGTTTCAAAAGA+GGG | 0.532340 | 4.3:-89706774 | None:intergenic |
| TGCCAAATCCTGCAAACCTA+AGG | 0.543122 | 4.3:+89705503 | MS.gene071005:CDS |
| GGTTGAGCACAATCAACTGC+AGG | 0.572742 | 4.3:+89706705 | MS.gene071005:CDS |
| CTGCAGGTGGACAAATCTGA+TGG | 0.593077 | 4.3:+89706721 | MS.gene071005:CDS |
| CTAACTTGGAAATTGTGGGA+GGG | 0.610138 | 4.3:+89706802 | MS.gene071005:CDS |
| GTCACACTCAAATATCTCAA+GGG | 0.610413 | 4.3:-89706601 | None:intergenic |
| AGGTAATACATCCGGTTATG+GGG | 0.613746 | 4.3:+89706854 | MS.gene071005:CDS |
| GGTCACACTCAAATATCTCA+AGG | 0.625085 | 4.3:-89706602 | None:intergenic |
| TTCATTTGTCACAATCTCCG+AGG | 0.636784 | 4.3:-89705382 | None:intergenic |
| TGAGCACAATCAACTGCAGG+TGG | 0.636879 | 4.3:+89706708 | MS.gene071005:CDS |
| TGTCATCAATTGCAGAACCT+CGG | 0.647597 | 4.3:+89705365 | None:intergenic |
| GATGCTAAGAAACTTCTACA+TGG | 0.648028 | 4.3:+89706648 | MS.gene071005:CDS |
| TCACACTCAAATATCTCAAG+GGG | 0.652492 | 4.3:-89706600 | None:intergenic |
| GCTAACTTGGAAATTGTGGG+AGG | 0.653551 | 4.3:+89706801 | MS.gene071005:CDS |
| ACAATGACTACAGAAAACAA+TGG | 0.653588 | 4.3:+89706671 | MS.gene071005:CDS |
| AGCCGAAATATTATTGAACA+CGG | 0.666524 | 4.3:-89706623 | None:intergenic |
| AAAACAATGGGATAAGACAG+TGG | 0.709971 | 4.3:+89706684 | MS.gene071005:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAATTTATTTATAAATTAAA+TGG | + | chr4.3:89706180-89706199 | MS.gene071005:intron | 0.0% |
| !!! | TATAATTAGTTATATGTTTT+AGG | + | chr4.3:89706459-89706478 | MS.gene071005:intron | 10.0% |
| !!! | TGTAATTTTAAATTTGAATT+TGG | + | chr4.3:89706516-89706535 | MS.gene071005:intron | 10.0% |
| !! | GACATTATTATAATTTATCA+TGG | + | chr4.3:89705556-89705575 | MS.gene071005:intron | 15.0% |
| !!! | AACATTTTACTGTTATATAA+AGG | + | chr4.3:89706834-89706853 | MS.gene071005:CDS | 15.0% |
| !!! | ATTAGAAACAATATTTTAAC+TGG | + | chr4.3:89706319-89706338 | MS.gene071005:intron | 15.0% |
| !!! | ATTTATATTTTTCCTTAACT+TGG | - | chr4.3:89705816-89705835 | None:intergenic | 15.0% |
| !! | CATTTCAAGAATGATATTAA+AGG | - | chr4.3:89706115-89706134 | None:intergenic | 20.0% |
| !! | GGAGTTTAAATTTAATTCTT+TGG | + | chr4.3:89706480-89706499 | MS.gene071005:intron | 20.0% |
| !! | TTATATAAAGGTAATACATC+CGG | + | chr4.3:89706846-89706865 | MS.gene071005:CDS | 20.0% |
| !!! | AAATGGAAGATATTTTTTGT+TGG | + | chr4.3:89706197-89706216 | MS.gene071005:intron | 20.0% |
| !!! | ATTTAAAATTACATGCTTGA+AGG | - | chr4.3:89706509-89706528 | None:intergenic | 20.0% |
| !!! | CTTTTTTGAATAAACATGTT+TGG | - | chr4.3:89706382-89706401 | None:intergenic | 20.0% |
| !!! | GAAGTTTTTAATGATGATTT+TGG | + | chr4.3:89705410-89705429 | MS.gene071005:CDS | 20.0% |
| !!! | TTTTTAATGATGATTTTGGA+TGG | + | chr4.3:89705414-89705433 | MS.gene071005:CDS | 20.0% |
| ! | AAAATTTGAATGAACTATGC+TGG | + | chr4.3:89706086-89706105 | MS.gene071005:intron | 25.0% |
| ! | ATCATATAACAATTCAAGCT+TGG | - | chr4.3:89705637-89705656 | None:intergenic | 25.0% |
| ! | ATCTAAATGAGAATGCTAAA+TGG | + | chr4.3:89705439-89705458 | MS.gene071005:CDS | 25.0% |
| ! | TCTAAATGAGAATGCTAAAT+GGG | + | chr4.3:89705440-89705459 | MS.gene071005:CDS | 25.0% |
| !! | AATCTATTTTGTCCAAGTTA+AGG | + | chr4.3:89705801-89705820 | MS.gene071005:intron | 25.0% |
| !!! | AATATTGTTTCTAATCAGCT+TGG | - | chr4.3:89706314-89706333 | None:intergenic | 25.0% |
| !!! | TAAGAAAAATTGACTTGTGT+AGG | - | chr4.3:89706441-89706460 | None:intergenic | 25.0% |
| AAAAAGGGAAAACATACAAG+GGG | - | chr4.3:89705671-89705690 | None:intergenic | 30.0% | |
| AAATACAAGTTTAGGGACAA+AGG | + | chr4.3:89706045-89706064 | MS.gene071005:intron | 30.0% | |
| ACAATGACTACAGAAAACAA+TGG | + | chr4.3:89706671-89706690 | MS.gene071005:CDS | 30.0% | |
| ACATTGTGCAAGTTAGAAAA+AGG | - | chr4.3:89705687-89705706 | None:intergenic | 30.0% | |
| ACCTCCTAAAATACAAGTTT+AGG | + | chr4.3:89706037-89706056 | MS.gene071005:intron | 30.0% | |
| AGAAAAAGGGAAAACATACA+AGG | - | chr4.3:89705673-89705692 | None:intergenic | 30.0% | |
| AGCCGAAATATTATTGAACA+CGG | - | chr4.3:89706626-89706645 | None:intergenic | 30.0% | |
| AGGATAAAATCAGTAACCTT+AGG | - | chr4.3:89705522-89705541 | None:intergenic | 30.0% | |
| ATAACAATTCAAGCTTGGTA+AGG | - | chr4.3:89705632-89705651 | None:intergenic | 30.0% | |
| ATTCTCTAGGTTTCAAAAGA+GGG | - | chr4.3:89706777-89706796 | None:intergenic | 30.0% | |
| CAATGACTACAGAAAACAAT+GGG | + | chr4.3:89706672-89706691 | MS.gene071005:CDS | 30.0% | |
| CATTGTGCAAGTTAGAAAAA+GGG | - | chr4.3:89705686-89705705 | None:intergenic | 30.0% | |
| CCTCCTAAAATACAAGTTTA+GGG | + | chr4.3:89706038-89706057 | MS.gene071005:intron | 30.0% | |
| GAAAAAGGGAAAACATACAA+GGG | - | chr4.3:89705672-89705691 | None:intergenic | 30.0% | |
| GACCGTGTTCAATAATATTT+CGG | + | chr4.3:89706621-89706640 | MS.gene071005:CDS | 30.0% | |
| TAATTTATCATGGCTACTCT+TGG | + | chr4.3:89705566-89705585 | MS.gene071005:intron | 30.0% | |
| TATTCTCTAGGTTTCAAAAG+AGG | - | chr4.3:89706778-89706797 | None:intergenic | 30.0% | |
| ! | TGTCCCTAAACTTGTATTTT+AGG | - | chr4.3:89706044-89706063 | None:intergenic | 30.0% |
| !!! | ATTTTATTGTTGTTTCTCCC+TGG | + | chr4.3:89706563-89706582 | MS.gene071005:intron | 30.0% |
| !!! | ATTTTTTGTTGGTGGTGTAA+GGG | + | chr4.3:89706208-89706227 | MS.gene071005:intron | 30.0% |
| !!! | TACTGATTTTATCCTTCAAC+TGG | + | chr4.3:89705527-89705546 | MS.gene071005:intron | 30.0% |
| !!! | TATTTTTTGTTGGTGGTGTA+AGG | + | chr4.3:89706207-89706226 | MS.gene071005:intron | 30.0% |
| !!! | TGGAAGATATTTTTTGTTGG+TGG | + | chr4.3:89706200-89706219 | MS.gene071005:intron | 30.0% |
| AAAACAATGGGATAAGACAG+TGG | + | chr4.3:89706684-89706703 | MS.gene071005:CDS | 35.0% | |
| AAAGGTAATACATCCGGTTA+TGG | + | chr4.3:89706852-89706871 | MS.gene071005:CDS | 35.0% | |
| AACAATAGCTCAACACCTAA+AGG | - | chr4.3:89706240-89706259 | None:intergenic | 35.0% | |
| AAGGTAATACATCCGGTTAT+GGG | + | chr4.3:89706853-89706872 | MS.gene071005:CDS | 35.0% | |
| AATGTCTATACTCCAGTTGA+AGG | - | chr4.3:89705542-89705561 | None:intergenic | 35.0% | |
| ACAATCAACCTATCAAGCAT+GGG | - | chr4.3:89706412-89706431 | None:intergenic | 35.0% | |
| ATGTGTCAAACACTCTTAAC+CGG | - | chr4.3:89706349-89706368 | None:intergenic | 35.0% | |
| GATGCTAAGAAACTTCTACA+TGG | + | chr4.3:89706648-89706667 | MS.gene071005:CDS | 35.0% | |
| GTCACACTCAAATATCTCAA+GGG | - | chr4.3:89706604-89706623 | None:intergenic | 35.0% | |
| TCACACTCAAATATCTCAAG+GGG | - | chr4.3:89706603-89706622 | None:intergenic | 35.0% | |
| !! | AAATCTGATGGTTCTGAAGT+TGG | + | chr4.3:89706733-89706752 | MS.gene071005:CDS | 35.0% |
| !! | CCCTAAACTTGTATTTTAGG+AGG | - | chr4.3:89706041-89706060 | None:intergenic | 35.0% |
| !!! | CAATATTTTAACTGGACACC+CGG | + | chr4.3:89706327-89706346 | MS.gene071005:intron | 35.0% |
| AACCTTAGGTTTGCAGGATT+TGG | - | chr4.3:89705508-89705527 | None:intergenic | 40.0% | |
| ACGTGCTAACTTGGAAATTG+TGG | + | chr4.3:89706797-89706816 | MS.gene071005:CDS | 40.0% | |
| AGGTAATACATCCGGTTATG+GGG | + | chr4.3:89706854-89706873 | MS.gene071005:CDS | 40.0% | |
| ATCAGTAACCTTAGGTTTGC+AGG | - | chr4.3:89705514-89705533 | None:intergenic | 40.0% | |
| CAAGTTAGCACGTATTCTCT+AGG | - | chr4.3:89706790-89706809 | None:intergenic | 40.0% | |
| CGTGCTAACTTGGAAATTGT+GGG | + | chr4.3:89706798-89706817 | MS.gene071005:CDS | 40.0% | |
| CTAACTTGGAAATTGTGGGA+GGG | + | chr4.3:89706802-89706821 | MS.gene071005:CDS | 40.0% | |
| CTAGAGAATACGTGCTAACT+TGG | + | chr4.3:89706788-89706807 | MS.gene071005:CDS | 40.0% | |
| CTGCATTTATGTCAGTGACT+AGG | - | chr4.3:89705719-89705738 | None:intergenic | 40.0% | |
| GACAATCAACCTATCAAGCA+TGG | - | chr4.3:89706413-89706432 | None:intergenic | 40.0% | |
| GGTCACACTCAAATATCTCA+AGG | - | chr4.3:89706605-89706624 | None:intergenic | 40.0% | |
| GTTTGTGTCTCAGCTGTATT+TGG | + | chr4.3:89706281-89706300 | MS.gene071005:intron | 40.0% | |
| TAACTTGGAAATTGTGGGAG+GGG | + | chr4.3:89706803-89706822 | MS.gene071005:CDS | 40.0% | |
| TAGGGACAAAGGATGAATAG+AGG | + | chr4.3:89706056-89706075 | MS.gene071005:intron | 40.0% | |
| TCAACTAAATGCTGCTCAGA+TGG | - | chr4.3:89705943-89705962 | None:intergenic | 40.0% | |
| TGTGTCAAACACTCTTAACC+GGG | - | chr4.3:89706348-89706367 | None:intergenic | 40.0% | |
| TTCATTTGTCACAATCTCCG+AGG | - | chr4.3:89705385-89705404 | None:intergenic | 40.0% | |
| ! | TCTAACTTGCACAATGTGCT+GGG | + | chr4.3:89705690-89705709 | MS.gene071005:intron | 40.0% |
| ! | TTCTAACTTGCACAATGTGC+TGG | + | chr4.3:89705689-89705708 | MS.gene071005:intron | 40.0% |
| !! | TAGGTGTTGAGCTATTGTTG+AGG | + | chr4.3:89706241-89706260 | MS.gene071005:intron | 40.0% |
| CTGAGCAGCATTTAGTTGAG+AGG | + | chr4.3:89705944-89705963 | MS.gene071005:intron | 45.0% | |
| GCTAACTTGGAAATTGTGGG+AGG | + | chr4.3:89706801-89706820 | MS.gene071005:CDS | 45.0% | |
| GTGTAAGGGTTGTGTCCTTT+AGG | + | chr4.3:89706222-89706241 | MS.gene071005:intron | 45.0% | |
| TAGTGAACAAAGTGTGCCAG+AGG | - | chr4.3:89705753-89705772 | None:intergenic | 45.0% | |
| TGCCAAATCCTGCAAACCTA+AGG | + | chr4.3:89705503-89705522 | MS.gene071005:CDS | 45.0% | |
| !! | TCTCAGCTGTATTTGGTGCA+TGG | + | chr4.3:89706288-89706307 | MS.gene071005:intron | 45.0% |
| AACTTGCACAATGTGCTGGG+AGG | + | chr4.3:89705693-89705712 | MS.gene071005:intron | 50.0% | |
| CTGCAGGTGGACAAATCTGA+TGG | + | chr4.3:89706721-89706740 | MS.gene071005:CDS | 50.0% | |
| GAACAAAGTGTGCCAGAGGA+TGG | - | chr4.3:89705749-89705768 | None:intergenic | 50.0% | |
| GGTTGAGCACAATCAACTGC+AGG | + | chr4.3:89706705-89706724 | MS.gene071005:CDS | 50.0% | |
| GTTGAGGCTGTGTGTCTAAG+AGG | + | chr4.3:89706257-89706276 | MS.gene071005:intron | 50.0% | |
| TCTCCCTGGATGTTGCAGAA+TGG | + | chr4.3:89706577-89706596 | MS.gene071005:intron | 50.0% | |
| TGAGCACAATCAACTGCAGG+TGG | + | chr4.3:89706708-89706727 | MS.gene071005:CDS | 50.0% | |
| TGCAGAGTCAAACCATCCTC+TGG | + | chr4.3:89705734-89705753 | MS.gene071005:intron | 50.0% | |
| TTGGAAATTGTGGGAGGGGA+AGG | + | chr4.3:89706807-89706826 | MS.gene071005:CDS | 50.0% | |
| !! | GAGAAGTGCCCATGCTTGAT+AGG | + | chr4.3:89706401-89706420 | MS.gene071005:intron | 50.0% |
| GGGCCATTCTGCAACATCCA+GGG | - | chr4.3:89706583-89706602 | None:intergenic | 55.0% | |
| GGGGCCATTCTGCAACATCC+AGG | - | chr4.3:89706584-89706603 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.3 | gene | 89705380 | 89706885 | 89705380 | ID=MS.gene071005 |
| chr4.3 | mRNA | 89705380 | 89706885 | 89705380 | ID=MS.gene071005.t1;Parent=MS.gene071005 |
| chr4.3 | exon | 89705380 | 89705524 | 89705380 | ID=MS.gene071005.t1.exon1;Parent=MS.gene071005.t1 |
| chr4.3 | CDS | 89705380 | 89705524 | 89705380 | ID=cds.MS.gene071005.t1;Parent=MS.gene071005.t1 |
| chr4.3 | exon | 89706595 | 89706885 | 89706595 | ID=MS.gene071005.t1.exon2;Parent=MS.gene071005.t1 |
| chr4.3 | CDS | 89706595 | 89706885 | 89706595 | ID=cds.MS.gene071005.t1;Parent=MS.gene071005.t1 |
| Gene Sequence |
| Protein sequence |
