AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene071128

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene071128.t1 MTR_4g045853 96.259 294 11 0 1 294 1 294 0.0 605
MS.gene071128.t1 MTR_2g089140 78.694 291 61 1 3 293 2 291 0.0 500
MS.gene071128.t1 MTR_2g089140 82.018 228 41 0 66 293 1 228 5.07e-149 417
MS.gene071128.t1 MTR_7g084750 49.660 294 132 4 7 292 6 291 3.35e-103 303
MS.gene071128.t1 MTR_7g084770 49.667 300 134 5 1 292 1 291 9.24e-103 302
MS.gene071128.t1 MTR_7g084760 50.000 296 131 5 1 288 1 287 2.16e-102 301
MS.gene071128.t1 MTR_4g097640 50.526 285 129 4 8 289 13 288 3.53e-102 300
MS.gene071128.t1 MTR_4g097670 50.526 285 129 4 8 289 13 288 3.53e-102 300
MS.gene071128.t1 MTR_6g033055 49.153 295 129 4 11 292 5 291 5.29e-102 300
MS.gene071128.t1 MTR_6g033085 48.822 297 131 4 9 292 3 291 2.23e-101 298
MS.gene071128.t1 MTR_4g097700 51.636 275 121 4 18 289 60 325 6.95e-100 296
MS.gene071128.t1 MTR_4g128410 48.944 284 133 4 8 288 6 280 5.01e-97 287
MS.gene071128.t1 MTR_3g084620 49.296 284 124 5 13 292 14 281 1.19e-95 283
MS.gene071128.t1 MTR_3g084630 49.296 284 124 5 13 292 14 281 1.33e-95 283
MS.gene071128.t1 MTR_7g093530 48.936 282 132 4 11 289 8 280 2.42e-94 280
MS.gene071128.t1 MTR_8g081040 48.239 284 129 5 11 290 14 283 8.92e-93 276
MS.gene071128.t1 MTR_4g128580 49.077 271 130 4 23 290 22 287 5.25e-91 272
MS.gene071128.t1 MTR_2g038860 46.690 287 145 4 10 292 8 290 4.01e-90 270
MS.gene071128.t1 MTR_2g038705 46.690 287 145 4 10 292 8 290 4.01e-90 270
MS.gene071128.t1 MTR_1g105900 44.948 287 145 5 8 288 12 291 4.57e-90 270
MS.gene071128.t1 MTR_4g126920 48.718 273 129 4 19 288 17 281 9.11e-90 269
MS.gene071128.t1 MTR_4g128570 47.586 290 115 6 14 289 7 273 2.77e-89 267
MS.gene071128.t1 MTR_6g088320 43.617 282 153 2 8 288 7 283 1.11e-88 266
MS.gene071128.t1 MTR_1g105495 46.021 289 138 5 11 288 13 294 1.43e-87 264
MS.gene071128.t1 MTR_8g102910 45.552 281 146 4 10 288 4 279 2.04e-85 258
MS.gene071128.t1 MTR_4g128400 48.207 251 120 3 40 289 43 284 2.04e-84 255
MS.gene071128.t1 MTR_8g102220 44.369 293 129 7 6 294 10 272 4.63e-82 249
MS.gene071128.t1 MTR_8g013055 42.857 266 141 4 27 290 31 287 1.04e-78 241
MS.gene071128.t1 MTR_4g128590 42.561 289 143 4 5 290 9 277 1.06e-78 240
MS.gene071128.t1 MTR_5g091730 42.857 287 136 6 11 288 11 278 7.31e-76 233
MS.gene071128.t1 MTR_3g089540 44.487 263 131 4 27 288 34 282 3.76e-75 232
MS.gene071128.t1 MTR_7g056090 41.392 273 145 6 8 277 13 273 1.71e-66 209
MS.gene071128.t1 MTR_2g049030 38.610 259 142 8 27 282 30 274 9.69e-59 189
MS.gene071128.t1 MTR_2g049130 38.735 253 140 7 27 276 29 269 6.90e-57 184
MS.gene071128.t1 MTR_7g102260 36.823 277 133 6 24 280 29 283 6.64e-56 182
MS.gene071128.t1 MTR_1g033420 36.364 297 166 8 5 289 7 292 6.60e-55 181
MS.gene071128.t1 MTR_1g063020 39.431 246 126 5 51 288 64 294 1.17e-54 179
MS.gene071128.t1 MTR_5g029100 37.450 251 147 4 43 288 60 305 4.49e-54 179
MS.gene071128.t1 MTR_2g095800 36.429 280 153 7 18 288 46 309 1.97e-53 176
MS.gene071128.t1 MTR_4g057450 35.878 262 145 5 27 280 50 296 4.98e-53 175
MS.gene071128.t1 MTR_8g090100 37.008 254 143 6 43 289 39 282 4.14e-52 174
MS.gene071128.t1 MTR_8g064180 35.271 258 150 5 40 288 61 310 9.06e-52 172
MS.gene071128.t1 MTR_5g029100 37.083 240 135 5 57 288 43 274 7.64e-51 170
MS.gene071128.t1 MTR_8g479340 41.176 102 53 2 187 288 41 135 5.53e-21 87.0
MS.gene071128.t1 MTR_8g032640 30.939 181 114 5 47 222 33 207 3.80e-12 64.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene071128.t1 AT4G03210 68.966 290 77 3 11 293 6 289 5.89e-156 437
MS.gene071128.t1 AT4G03210 73.418 237 57 2 57 293 31 261 7.02e-138 390
MS.gene071128.t1 AT5G13870 48.649 296 140 4 1 292 4 291 2.06e-104 306
MS.gene071128.t1 AT5G13870 48.649 296 140 4 1 292 4 291 2.06e-104 306
MS.gene071128.t1 AT5G13870 48.649 296 140 4 1 292 4 291 2.06e-104 306
MS.gene071128.t1 AT4G37800 49.826 287 129 5 8 288 12 289 5.72e-101 298
MS.gene071128.t1 AT5G65730 49.648 284 130 5 8 288 17 290 2.52e-99 293
MS.gene071128.t1 AT3G23730 49.825 285 133 4 11 292 13 290 1.52e-97 289
MS.gene071128.t1 AT2G06850 48.881 268 127 3 27 292 35 294 2.68e-94 281
MS.gene071128.t1 AT4G14130 49.492 295 135 7 2 292 4 288 7.22e-94 279
MS.gene071128.t1 AT4G25810 46.290 283 141 5 11 289 9 284 5.15e-91 272
MS.gene071128.t1 AT5G57550 46.392 291 135 5 9 290 6 284 2.67e-90 270
MS.gene071128.t1 AT5G57530 48.708 271 126 6 21 288 22 282 3.01e-90 270
MS.gene071128.t1 AT5G57540 50.598 251 114 4 39 288 40 281 3.62e-90 270
MS.gene071128.t1 AT5G57560 44.746 295 138 5 5 289 3 282 3.91e-90 270
MS.gene071128.t1 AT1G11545 47.475 297 136 7 3 288 12 299 1.50e-88 266
MS.gene071128.t1 AT4G28850 45.230 283 144 3 13 288 9 287 2.63e-88 265
MS.gene071128.t1 AT2G06850 50.000 238 111 2 55 292 5 234 6.26e-88 262
MS.gene071128.t1 AT2G14620 45.105 286 144 5 9 288 16 294 2.01e-87 263
MS.gene071128.t1 AT4G25820 46.886 273 133 4 19 288 22 285 8.95e-85 256
MS.gene071128.t1 AT4G30270 43.836 292 135 6 1 289 1 266 9.12e-85 255
MS.gene071128.t1 AT2G14620 48.291 234 113 3 56 288 18 244 2.41e-82 249
MS.gene071128.t1 AT2G18800 45.302 298 153 7 1 289 1 297 3.19e-81 248
MS.gene071128.t1 AT2G14620 48.444 225 108 3 65 288 16 233 8.16e-80 242
MS.gene071128.t1 AT5G48070 46.748 246 117 4 44 288 49 281 1.22e-79 243
MS.gene071128.t1 AT4G13090 41.156 294 158 5 3 288 2 288 5.83e-79 241
MS.gene071128.t1 AT3G25050 42.803 264 140 5 27 288 35 289 2.30e-76 235
MS.gene071128.t1 AT4G13080 43.939 264 135 5 27 288 39 291 6.26e-73 226
MS.gene071128.t1 AT4G30290 44.017 234 117 4 56 288 56 276 1.94e-70 219
MS.gene071128.t1 AT4G30280 44.017 234 117 4 56 288 61 281 2.02e-69 217
MS.gene071128.t1 AT1G65310 43.404 235 119 4 56 289 61 282 2.15e-69 217
MS.gene071128.t1 AT1G65310 43.404 235 119 4 56 289 83 304 2.96e-69 217
MS.gene071128.t1 AT3G44990 39.560 273 142 5 24 288 36 293 1.28e-60 194
MS.gene071128.t1 AT1G32170 38.435 294 159 9 9 289 10 294 6.62e-60 194
MS.gene071128.t1 AT1G10550 37.154 253 148 5 44 288 60 309 2.01e-55 182
MS.gene071128.t1 AT2G36870 37.591 274 146 7 24 288 40 297 9.04e-55 180
MS.gene071128.t1 AT1G14720 34.797 296 175 7 3 289 5 291 2.66e-54 179
MS.gene071128.t1 AT2G01850 35.235 298 171 7 3 289 5 291 4.07e-54 179
MS.gene071128.t1 AT4G18990 35.273 275 153 6 36 289 43 313 4.21e-52 174
MS.gene071128.t1 AT3G48580 37.190 242 129 8 47 288 55 273 4.40e-47 159
MS.gene071128.t1 AT3G48580 36.325 234 126 8 55 288 4 214 1.51e-44 151
MS.gene071128.t1 AT4G18990 35.784 204 117 4 96 289 40 239 1.29e-41 145
MS.gene071128.t1 AT3G48580 30.992 242 124 8 47 288 55 253 1.28e-30 115
MS.gene071128.t1 AT2G36870 40.288 139 75 2 24 154 40 178 4.15e-28 107

Find 64 sgRNAs with CRISPR-Local

Find 152 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGTTATCAAGTTTGAGTTTA+AGG 0.041776 4.3:+51921620 None:intergenic
ATGTTTGTGTGTACCCTTAT+TGG 0.226363 4.3:+51919216 None:intergenic
GGCTTTGTAGGTTGCAATAA+AGG 0.238238 4.3:+51919074 None:intergenic
CCCAAACATATACTTGCTTT+TGG 0.249364 4.3:+51921403 None:intergenic
ATTGGTTGGTCCTTTGGATA+TGG 0.256401 4.3:+51919177 None:intergenic
ACATTTGTTTGCACAGAATA+AGG 0.263774 4.3:+51920618 None:intergenic
ATTATTGTAGTGATGGTTCT+AGG 0.279226 4.3:-51918879 MS.gene071128:CDS
ACTCCCATTGGTTGGTCCTT+TGG 0.294306 4.3:+51919171 None:intergenic
TCCAAAAGCAAGTATATGTT+TGG 0.311980 4.3:-51921404 MS.gene071128:CDS
TAGTGGATGAAACACCAATA+AGG 0.312627 4.3:-51919230 MS.gene071128:CDS
TCCATTGCAGCCACTGAAAC+TGG 0.316411 4.3:+51919027 None:intergenic
ACACACTCAACAGGAGTAAT+AGG 0.318635 4.3:+51918853 None:intergenic
ATTGATTTCAAAGGCTTTGT+AGG 0.332906 4.3:+51919062 None:intergenic
CTCAAACTTGATAACTCTTC+TGG 0.342527 4.3:-51921613 MS.gene071128:intron
GATCTCCTTCATGGAGAAAA+TGG 0.350214 4.3:+51921644 None:intergenic
AAATCAAACTCGTTGTGGTT+TGG 0.361017 4.3:+51920669 None:intergenic
GTTTAAGGAGATCTCCTTCA+TGG 0.368795 4.3:+51921635 None:intergenic
TGGGAGTATATAGTTCAATA+TGG 0.384156 4.3:-51919155 MS.gene071128:CDS
GTGGGTTAAGAATCATCATA+TGG 0.413348 4.3:-51918908 MS.gene071128:CDS
AGTGGATGAAACACCAATAA+GGG 0.419179 4.3:-51919229 MS.gene071128:CDS
GCTTTGTAGGTTGCAATAAA+GGG 0.420743 4.3:+51919075 None:intergenic
GTAATGAAGATAAGAAATAT+TGG 0.427156 4.3:-51918984 MS.gene071128:CDS
ACACACAAACATGGAACATA+AGG 0.435591 4.3:-51919205 MS.gene071128:CDS
CCAAAAGCAAGTATATGTTT+GGG 0.446940 4.3:-51921403 MS.gene071128:CDS
ATCAAAGTCATCAACTTATG+TGG 0.447459 4.3:-51918927 MS.gene071128:CDS
AATGTGTATGTGAATGGAAT+TGG 0.448488 4.3:-51920600 MS.gene071128:CDS
ATCCAGCTTAAGCTTGTTGA+AGG 0.448965 4.3:-51921371 MS.gene071128:CDS
GTGAGCAAAGACTTAACCTT+TGG 0.449716 4.3:-51920572 MS.gene071128:CDS
CGATGATGGACACACTCAAC+AGG 0.451833 4.3:+51918844 None:intergenic
CAATATGGAATGCAGATGAT+TGG 0.451938 4.3:-51919140 MS.gene071128:CDS
CTCACAAGCATTGATTTCAA+AGG 0.475544 4.3:+51919053 None:intergenic
AATATGGAATGCAGATGATT+GGG 0.477902 4.3:-51919139 MS.gene071128:CDS
CACCTTCAACAAGCTTAAGC+TGG 0.489730 4.3:+51921369 None:intergenic
CTTGTTGAAGGTGACTCAGC+TGG 0.494006 4.3:-51921359 MS.gene071128:CDS
CAAACAAATGTGTATGTGAA+TGG 0.495017 4.3:-51920606 MS.gene071128:CDS
TCCAGTTTCAGTGGCTGCAA+TGG 0.519854 4.3:-51919028 MS.gene071128:CDS
AGAGTAAGAGTGAAAATCCT+TGG 0.520212 4.3:+51920539 None:intergenic
GTTTATGATTATTGTAGTGA+TGG 0.523389 4.3:-51918886 MS.gene071128:CDS
TAAGAGTGAAAATCCTTGGT+AGG 0.527500 4.3:+51920543 None:intergenic
CTAGCTATGTATGACGATGA+TGG 0.532008 4.3:+51918830 None:intergenic
CACACAAACATGGAACATAA+GGG 0.533729 4.3:-51919204 MS.gene071128:CDS
AACTATATACTCCCATTGGT+TGG 0.534934 4.3:+51919163 None:intergenic
ATTCAAAATCAAACTCGTTG+TGG 0.538842 4.3:+51920664 None:intergenic
ATTGAACTATATACTCCCAT+TGG 0.544274 4.3:+51919159 None:intergenic
TATCCAAAGGACCAACCAAT+GGG 0.553638 4.3:-51919174 MS.gene071128:CDS
AAATGGTCAAAAGCCCAACT+TGG 0.561534 4.3:+51921661 None:intergenic
GAGGAAGAGTGAAAACAGAT+TGG 0.562933 4.3:-51919107 MS.gene071128:CDS
TGAAGATAAGAAATATTGGT+GGG 0.579001 4.3:-51918980 MS.gene071128:CDS
AAGTTCATCAAACTTGGAAG+AGG 0.579984 4.3:+51921690 None:intergenic
AAAGGTTAAGTCTTTGCTCA+CGG 0.581166 4.3:+51920574 None:intergenic
TCAAAGTCATCAACTTATGT+GGG 0.588263 4.3:-51918926 MS.gene071128:CDS
ATGAAGATAAGAAATATTGG+TGG 0.588757 4.3:-51918981 MS.gene071128:CDS
AAGAGTGAAAATCCTTGGTA+GGG 0.591421 4.3:+51920544 None:intergenic
CCTTGGTAGGGTCAAACCAA+AGG 0.593403 4.3:+51920556 None:intergenic
TTGTGAGTGTCCAGTTTCAG+TGG 0.599803 4.3:-51919037 MS.gene071128:CDS
GTGATATAGATGTCATCAGA+GGG 0.614058 4.3:-51920693 MS.gene071128:intron
ATATCCAAAGGACCAACCAA+TGG 0.618347 4.3:-51919175 MS.gene071128:CDS
AATAAGGGTACACACAAACA+TGG 0.625212 4.3:-51919214 MS.gene071128:CDS
GCAGATGATTGGGCAACACA+AGG 0.626520 4.3:-51919129 MS.gene071128:CDS
CCTTTGGTTTGACCCTACCA+AGG 0.640836 4.3:-51920556 MS.gene071128:CDS
TAAGGGTATACCATATCCAA+AGG 0.644360 4.3:-51919187 MS.gene071128:CDS
TGTGATATAGATGTCATCAG+AGG 0.645456 4.3:-51920694 MS.gene071128:intron
CTGCAATGGATAATACAAAG+AGG 0.674798 4.3:-51919014 MS.gene071128:CDS
GATGATTGGGCAACACAAGG+AGG 0.684173 4.3:-51919126 MS.gene071128:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CATTGAATATATTAAAATAT+TGG + chr4.3:51921119-51921138 None:intergenic 10.0%
!! TTGAATATATTAAAATATTG+GGG + chr4.3:51921117-51921136 None:intergenic 10.0%
!!! ACAAAAATATGTTTAAAATA+TGG + chr4.3:51919114-51919133 None:intergenic 10.0%
!!! ATATTTTGATTAAATAACAA+TGG - chr4.3:51919137-51919156 MS.gene071128:CDS 10.0%
!!! ATTTTAATATATTCAATGAT+TGG - chr4.3:51921121-51921140 MS.gene071128:intron 10.0%
!!! CTTTATACTAAATTAATTTT+TGG - chr4.3:51919753-51919772 MS.gene071128:intron 10.0%
!!! TATTTTTCATAAAAAAAGAA+AGG - chr4.3:51920546-51920565 MS.gene071128:CDS 10.0%
!! AAGAGAATAAAATATAAGAA+AGG - chr4.3:51920571-51920590 MS.gene071128:CDS 15.0%
!! AGAGAATAAAATATAAGAAA+GGG - chr4.3:51920572-51920591 MS.gene071128:CDS 15.0%
!! TTACTAAAGCAAATATTAAT+TGG + chr4.3:51919671-51919690 None:intergenic 15.0%
!!! AAAAATATTACTTTTTGACA+AGG + chr4.3:51920422-51920441 None:intergenic 15.0%
!!! ATTTTTTAACCTTTATCATA+AGG - chr4.3:51920879-51920898 MS.gene071128:intron 15.0%
!!! TATTTTTTAATCTAATGTGA+AGG - chr4.3:51919385-51919404 MS.gene071128:intron 15.0%
!!! TTTTTTAACCTTTATCATAA+GGG - chr4.3:51920880-51920899 MS.gene071128:intron 15.0%
!! ACAAATCAATGGAAAATAAA+AGG - chr4.3:51920721-51920740 MS.gene071128:intron 20.0%
!! GTAATGAAGATAAGAAATAT+TGG - chr4.3:51921607-51921626 MS.gene071128:intron 20.0%
!! TATTCATTCATAATATTCTG+TGG - chr4.3:51919784-51919803 MS.gene071128:intron 20.0%
!! TTTCTCAATATACTAAAGAA+TGG + chr4.3:51919322-51919341 None:intergenic 20.0%
!!! AAATTAAGCACTGAAAAATT+AGG + chr4.3:51921642-51921661 None:intergenic 20.0%
!!! AATGCTTTATTTTTCTCTAA+TGG - chr4.3:51920641-51920660 MS.gene071128:CDS 20.0%
!!! AATTTTTGCAGAAATTACAA+AGG + chr4.3:51920827-51920846 None:intergenic 20.0%
!!! ATTTAGGCTTTCTAAAATTT+AGG - chr4.3:51921211-51921230 MS.gene071128:intron 20.0%
!!! GAACTGTATATTATTGATTA+GGG + chr4.3:51919551-51919570 None:intergenic 20.0%
!!! GAGTTTGATTTTGAATTTTT+AGG - chr4.3:51919934-51919953 MS.gene071128:intron 20.0%
!!! GTAATTTCTGCAAAAATTTT+TGG - chr4.3:51920829-51920848 MS.gene071128:intron 20.0%
!!! TAACCTTAGCAATTTAATTT+AGG - chr4.3:51921195-51921214 MS.gene071128:intron 20.0%
!!! TGAACTGTATATTATTGATT+AGG + chr4.3:51919552-51919571 None:intergenic 20.0%
! AAGCCTAAATTAAATTGCTA+AGG + chr4.3:51921201-51921220 None:intergenic 25.0%
! ACTCATACGATTATAAAGTA+GGG + chr4.3:51919706-51919725 None:intergenic 25.0%
! AGAGTTGTGAAAAATAACTA+GGG + chr4.3:51919043-51919062 None:intergenic 25.0%
! ATATAAGAAAGGGAATAATG+AGG - chr4.3:51920582-51920601 MS.gene071128:CDS 25.0%
! ATGAAGATAAGAAATATTGG+TGG - chr4.3:51921610-51921629 MS.gene071128:intron 25.0%
! CTGTCATATAATAATAACTG+TGG + chr4.3:51919637-51919656 None:intergenic 25.0%
! GTTTATGATTATTGTAGTGA+TGG - chr4.3:51921705-51921724 MS.gene071128:CDS 25.0%
! TAGAGTTGTGAAAAATAACT+AGG + chr4.3:51919044-51919063 None:intergenic 25.0%
! TATTCTGCAATTAACCAATT+AGG + chr4.3:51920779-51920798 None:intergenic 25.0%
! TGAAGATAAGAAATATTGGT+GGG - chr4.3:51921611-51921630 MS.gene071128:intron 25.0%
! TGTATGTGTATGAATTATTG+AGG - chr4.3:51919830-51919849 MS.gene071128:intron 25.0%
! TTGAAAAAGTTCATCAAACT+TGG + chr4.3:51918910-51918929 None:intergenic 25.0%
! TTGATTAAATAACAATGGCA+TGG - chr4.3:51919142-51919161 MS.gene071128:CDS 25.0%
! TTTATGTCAATACTCCTAAT+TGG - chr4.3:51920762-51920781 MS.gene071128:intron 25.0%
!! AGTTATCAAGTTTGAGTTTA+AGG + chr4.3:51918974-51918993 None:intergenic 25.0%
!! CCATTGATGACAAAATTAAA+GGG + chr4.3:51919509-51919528 None:intergenic 25.0%
!! TATTTTTCACAACTCTATCA+AGG - chr4.3:51919047-51919066 MS.gene071128:CDS 25.0%
!! TCCATTGATGACAAAATTAA+AGG + chr4.3:51919510-51919529 None:intergenic 25.0%
!!! TCAAAAATGTGTTGGTTTTT+TGG - chr4.3:51918840-51918859 MS.gene071128:CDS 25.0%
!!! TTGAACAATCAGATTTTTAG+TGG - chr4.3:51921344-51921363 MS.gene071128:CDS 25.0%
!!! TTTTTTTACTGTTGATTGAG+TGG + chr4.3:51920907-51920926 None:intergenic 25.0%
AATATGGAATGCAGATGATT+GGG - chr4.3:51921452-51921471 MS.gene071128:intron 30.0%
AATATTGGGGCAAGAAAATA+TGG + chr4.3:51921104-51921123 None:intergenic 30.0%
AATGTGTATGTGAATGGAAT+TGG - chr4.3:51919991-51920010 MS.gene071128:intron 30.0%
ACATTTGTTTGCACAGAATA+AGG + chr4.3:51919976-51919995 None:intergenic 30.0%
ATCAAAGTCATCAACTTATG+TGG - chr4.3:51921664-51921683 MS.gene071128:CDS 30.0%
ATTATTGTAGTGATGGTTCT+AGG - chr4.3:51921712-51921731 MS.gene071128:CDS 30.0%
ATTCAAAATCAAACTCGTTG+TGG + chr4.3:51919930-51919949 None:intergenic 30.0%
ATTGAACTATATACTCCCAT+TGG + chr4.3:51921435-51921454 None:intergenic 30.0%
CAAACAAATGTGTATGTGAA+TGG - chr4.3:51919985-51920004 MS.gene071128:intron 30.0%
CAATGGAAAATAAAAGGTGA+TGG - chr4.3:51920727-51920746 MS.gene071128:intron 30.0%
CCAAAAGCAAGTATATGTTT+GGG - chr4.3:51919188-51919207 MS.gene071128:CDS 30.0%
GAATTATGTACCCAACTTAT+GGG + chr4.3:51921240-51921259 None:intergenic 30.0%
GACTCATACGATTATAAAGT+AGG + chr4.3:51919707-51919726 None:intergenic 30.0%
GCAAATATTAATTGGACAAG+AGG + chr4.3:51919663-51919682 None:intergenic 30.0%
GTCACTCAAATACAAATCAA+TGG - chr4.3:51920710-51920729 MS.gene071128:intron 30.0%
TCAAAGTCATCAACTTATGT+GGG - chr4.3:51921665-51921684 MS.gene071128:CDS 30.0%
TCCAAAAGCAAGTATATGTT+TGG - chr4.3:51919187-51919206 MS.gene071128:CDS 30.0%
TGAATTATGTACCCAACTTA+TGG + chr4.3:51921241-51921260 None:intergenic 30.0%
TTCTTCACATAGTACTAACA+TGG - chr4.3:51920171-51920190 MS.gene071128:intron 30.0%
! ATTAGAGTGAGAGTGTAATA+AGG + chr4.3:51920111-51920130 None:intergenic 30.0%
! ATTGATTTCAAAGGCTTTGT+AGG + chr4.3:51921532-51921551 None:intergenic 30.0%
! TGGGAGTATATAGTTCAATA+TGG - chr4.3:51921436-51921455 MS.gene071128:intron 30.0%
! TGTTCAACATTTTCTTCACA+TGG - chr4.3:51919076-51919095 MS.gene071128:CDS 30.0%
! TTCACTCTTACTCTATTTTC+TGG - chr4.3:51920061-51920080 MS.gene071128:intron 30.0%
!! CCCTTTAATTTTGTCATCAA+TGG - chr4.3:51919506-51919525 MS.gene071128:intron 30.0%
!!! ATGAACTTTTTCAACCAAGT+TGG - chr4.3:51918916-51918935 MS.gene071128:CDS 30.0%
!!! GCTTTATTTTTCTCTAATGG+TGG - chr4.3:51920644-51920663 MS.gene071128:CDS 30.0%
!!! TGAACTTTTTCAACCAAGTT+GGG - chr4.3:51918917-51918936 MS.gene071128:CDS 30.0%
AAAATAACTAGGGTTGTGGT+AGG + chr4.3:51919033-51919052 None:intergenic 35.0%
AAAGGTTAAGTCTTTGCTCA+CGG + chr4.3:51920020-51920039 None:intergenic 35.0%
AAATCAAACTCGTTGTGGTT+TGG + chr4.3:51919925-51919944 None:intergenic 35.0%
AACTATATACTCCCATTGGT+TGG + chr4.3:51921431-51921450 None:intergenic 35.0%
AAGAAAGGGAATAATGAGGT+TGG - chr4.3:51920586-51920605 MS.gene071128:CDS 35.0%
AAGAGTGAAAATCCTTGGTA+GGG + chr4.3:51920050-51920069 None:intergenic 35.0%
AAGTTCATCAAACTTGGAAG+AGG + chr4.3:51918904-51918923 None:intergenic 35.0%
ACACACAAACATGGAACATA+AGG - chr4.3:51921386-51921405 MS.gene071128:CDS 35.0%
AGAGTAAGAGTGAAAATCCT+TGG + chr4.3:51920055-51920074 None:intergenic 35.0%
AGGTTAAAAAATGTTGCGCA+TGG + chr4.3:51920871-51920890 None:intergenic 35.0%
AGTGGATGAAACACCAATAA+GGG - chr4.3:51921362-51921381 MS.gene071128:CDS 35.0%
ATGACAATCAAAGGCTATGA+TGG + chr4.3:51920675-51920694 None:intergenic 35.0%
ATGTTTGTGTGTACCCTTAT+TGG + chr4.3:51921378-51921397 None:intergenic 35.0%
ATTTGTATTTGAGTGACCCA+CGG + chr4.3:51920707-51920726 None:intergenic 35.0%
CAATATGGAATGCAGATGAT+TGG - chr4.3:51921451-51921470 MS.gene071128:intron 35.0%
CACACAAACATGGAACATAA+GGG - chr4.3:51921387-51921406 MS.gene071128:CDS 35.0%
CTGCAATGGATAATACAAAG+AGG - chr4.3:51921577-51921596 MS.gene071128:intron 35.0%
GAGTCTGAAAAGGTTGTAAA+AGG - chr4.3:51920611-51920630 MS.gene071128:CDS 35.0%
GCAAACTAACATGTTCCTTT+AGG + chr4.3:51921082-51921101 None:intergenic 35.0%
GCTTTGTAGGTTGCAATAAA+GGG + chr4.3:51921519-51921538 None:intergenic 35.0%
GTGAAAAATAACTAGGGTTG+TGG + chr4.3:51919037-51919056 None:intergenic 35.0%
GTGATATAGATGTCATCAGA+GGG - chr4.3:51919898-51919917 MS.gene071128:intron 35.0%
TAAGAGTGAAAATCCTTGGT+AGG + chr4.3:51920051-51920070 None:intergenic 35.0%
TGTGATATAGATGTCATCAG+AGG - chr4.3:51919897-51919916 MS.gene071128:intron 35.0%
! AATAAGGGTACACACAAACA+TGG - chr4.3:51921377-51921396 MS.gene071128:CDS 35.0%
! AATTTAGGATGCCCATAAGT+TGG - chr4.3:51921226-51921245 MS.gene071128:intron 35.0%
! ATTATTGATTAGGGTCCAAG+AGG + chr4.3:51919542-51919561 None:intergenic 35.0%
! ATTTAGGATGCCCATAAGTT+GGG - chr4.3:51921227-51921246 MS.gene071128:intron 35.0%
! CCCAAACATATACTTGCTTT+TGG + chr4.3:51919191-51919210 None:intergenic 35.0%
! CTCAAACTTGATAACTCTTC+TGG - chr4.3:51918978-51918997 MS.gene071128:CDS 35.0%
! GTGGGTTAAGAATCATCATA+TGG - chr4.3:51921683-51921702 MS.gene071128:CDS 35.0%
! TAAGGGTATACCATATCCAA+AGG - chr4.3:51921404-51921423 MS.gene071128:CDS 35.0%
! TAGTGGATGAAACACCAATA+AGG - chr4.3:51921361-51921380 MS.gene071128:CDS 35.0%
! TTTGACCATTTTCTCCATGA+AGG - chr4.3:51918942-51918961 MS.gene071128:CDS 35.0%
!! AACATGTTCCTTTAGGACAT+TGG + chr4.3:51921075-51921094 None:intergenic 35.0%
!! ACATGTTCCTTTAGGACATT+GGG + chr4.3:51921074-51921093 None:intergenic 35.0%
!! CTCACAAGCATTGATTTCAA+AGG + chr4.3:51921541-51921560 None:intergenic 35.0%
!! GAATTTTTAGGCAACACAAC+AGG - chr4.3:51919946-51919965 MS.gene071128:intron 35.0%
AAATGGTCAAAAGCCCAACT+TGG + chr4.3:51918933-51918952 None:intergenic 40.0%
ACACACTCAACAGGAGTAAT+AGG + chr4.3:51921741-51921760 None:intergenic 40.0%
ATATCCAAAGGACCAACCAA+TGG - chr4.3:51921416-51921435 MS.gene071128:CDS 40.0%
GAGGAAGAGTGAAAACAGAT+TGG - chr4.3:51921484-51921503 MS.gene071128:intron 40.0%
GATCTCCTTCATGGAGAAAA+TGG + chr4.3:51918950-51918969 None:intergenic 40.0%
GGGAACACAATGACAATCAA+AGG + chr4.3:51920684-51920703 None:intergenic 40.0%
GTGAATACCCAATGTCCTAA+AGG - chr4.3:51921064-51921083 MS.gene071128:intron 40.0%
GTGAGCAAAGACTTAACCTT+TGG - chr4.3:51920019-51920038 MS.gene071128:intron 40.0%
GTTTAAGGAGATCTCCTTCA+TGG + chr4.3:51918959-51918978 None:intergenic 40.0%
TATCCAAAGGACCAACCAAT+GGG - chr4.3:51921417-51921436 MS.gene071128:CDS 40.0%
TGAGTGGTCCCTTATGATAA+AGG + chr4.3:51920891-51920910 None:intergenic 40.0%
TTTGTATTTGAGTGACCCAC+GGG + chr4.3:51920706-51920725 None:intergenic 40.0%
! ATTGGTTGGTCCTTTGGATA+TGG + chr4.3:51921417-51921436 None:intergenic 40.0%
! GGCTTTGTAGGTTGCAATAA+AGG + chr4.3:51921520-51921539 None:intergenic 40.0%
! GTGTTTCTAACATGTGTGCT+TGG - chr4.3:51920967-51920986 MS.gene071128:intron 40.0%
!! ATCCAGCTTAAGCTTGTTGA+AGG - chr4.3:51919220-51919239 MS.gene071128:CDS 40.0%
AATGGAGATGCTCTACCTCT+TGG - chr4.3:51919524-51919543 MS.gene071128:intron 45.0%
CACCTTCAACAAGCTTAAGC+TGG + chr4.3:51919225-51919244 None:intergenic 45.0%
GAGGTTGGATGAGTCTGAAA+AGG - chr4.3:51920601-51920620 MS.gene071128:CDS 45.0%
GATACAAAACCAGCACCTGA+AGG + chr4.3:51919169-51919188 None:intergenic 45.0%
TAACAATGGCATGGACCTTC+AGG - chr4.3:51919151-51919170 MS.gene071128:CDS 45.0%
TTGTATTTGAGTGACCCACG+GGG + chr4.3:51920705-51920724 None:intergenic 45.0%
TTGTGAGTGTCCAGTTTCAG+TGG - chr4.3:51921554-51921573 MS.gene071128:intron 45.0%
!! ATTGAATATATTAAAATATT+GGG + chr4.3:51921118-51921137 None:intergenic 5.0%
!!! AATTTTTTACATTTATATTT+TGG - chr4.3:51919585-51919604 MS.gene071128:intron 5.0%
ATTGTCATTGTGTTCCCCCG+TGG - chr4.3:51920687-51920706 MS.gene071128:CDS 50.0%
CCTTGGTAGGGTCAAACCAA+AGG + chr4.3:51920038-51920057 None:intergenic 50.0%
CTTGTTGAAGGTGACTCAGC+TGG - chr4.3:51919232-51919251 MS.gene071128:CDS 50.0%
GATGATTGGGCAACACAAGG+AGG - chr4.3:51921465-51921484 MS.gene071128:intron 50.0%
GCAGATGATTGGGCAACACA+AGG - chr4.3:51921462-51921481 MS.gene071128:intron 50.0%
TCCAGTTTCAGTGGCTGCAA+TGG - chr4.3:51921563-51921582 MS.gene071128:intron 50.0%
TCCATTGCAGCCACTGAAAC+TGG + chr4.3:51921567-51921586 None:intergenic 50.0%
TGTATTTGAGTGACCCACGG+GGG + chr4.3:51920704-51920723 None:intergenic 50.0%
TTGTCATTGTGTTCCCCCGT+GGG - chr4.3:51920688-51920707 MS.gene071128:intron 50.0%
! ACTCCCATTGGTTGGTCCTT+TGG + chr4.3:51921423-51921442 None:intergenic 50.0%
!! CCTTTGGTTTGACCCTACCA+AGG - chr4.3:51920035-51920054 MS.gene071128:intron 50.0%
!! CGATGATGGACACACTCAAC+AGG + chr4.3:51921750-51921769 None:intergenic 50.0%
TGGCATGGACCTTCAGGTGC+TGG - chr4.3:51919157-51919176 MS.gene071128:CDS 60.0%


Chromosome Type Strat End Strand Name
chr4.3 gene 51918834 51921779 51918834 ID=MS.gene071128
chr4.3 mRNA 51918834 51921779 51918834 ID=MS.gene071128.t1;Parent=MS.gene071128
chr4.3 exon 51921614 51921779 51921614 ID=MS.gene071128.t1.exon1;Parent=MS.gene071128.t1
chr4.3 CDS 51921614 51921779 51921614 ID=cds.MS.gene071128.t1;Parent=MS.gene071128.t1
chr4.3 exon 51921340 51921440 51921340 ID=MS.gene071128.t1.exon2;Parent=MS.gene071128.t1
chr4.3 CDS 51921340 51921440 51921340 ID=cds.MS.gene071128.t1;Parent=MS.gene071128.t1
chr4.3 exon 51920513 51920706 51920513 ID=MS.gene071128.t1.exon3;Parent=MS.gene071128.t1
chr4.3 CDS 51920513 51920706 51920513 ID=cds.MS.gene071128.t1;Parent=MS.gene071128.t1
chr4.3 exon 51918834 51919257 51918834 ID=MS.gene071128.t1.exon4;Parent=MS.gene071128.t1
chr4.3 CDS 51918834 51919257 51918834 ID=cds.MS.gene071128.t1;Parent=MS.gene071128.t1
Gene Sequence

>MS.gene071128

ATGTCTTCAAAAATGTGTTGGTTTTTTGGTTTGTTTGTGTGTCTTATGTTAAGTGTGTCTTTGTCTACCTCTTCCAAGTTTGATGAACTTTTTCAACCAAGTTGGGCTTTTGACCATTTTCTCCATGAAGGAGATCTCCTTAAACTCAAACTTGATAACTCTTCTGGTAAACACATCAGTTCTTTGTAAACTTTTTCCTACCACAACCCTAGTTATTTTTCACAACTCTATCAAGGCATTTTTGTTCAACATTTTCTTCACATGGCAATAATTTAATCCATATTTTAAACATATTTTTGTAAAATATTTTGATTAAATAACAATGGCATGGACCTTCAGGTGCTGGTTTTGTATCCAAAAGCAAGTATATGTTTGGGAGAGTATCTATCCAGCTTAAGCTTGTTGAAGGTGACTCAGCTGGAACTGTTACTGCTTTCTATGTAAGTGAATTAATTAAATATAAATTAAAATCTATTATTTTATTACCATTCTTTAGTATATTGAGAAAAAAATTCATCAAGACTATACAAAATACACTCTTATTTTTTATTTATTTTTTAATCTAATGTGAAGGAAGATCAAGAATATATTTTAACTTTGAAGTGATATGAAAAAAAAATATCAATTCAAAATACATACTATTTGATGTGTCTATCTTTGATTTACTATTCACCCTTTAATTTTGTCATCAATGGAGATGCTCTACCTCTTGGACCCTAATCAATAATATACAGTTCATTCAAGTGCAAGAAATTTTTTACATTTATATTTTGGTACTCAATGAAAAGACTACTATAGTACCACAGTTATTATTATATGACAGCTACCTCTTGTCCAATTAATATTTGCTTTAGTAACTTTTTTTTCTTCCCTACTTTATAATCGTATGAGTCAAAAAACAAAGCACAAATCATCAATACTTTATACTAAATTAATTTTTGGTGATTTTATATTCATTCATAATATTCTGTGGTTGAATTGACATTGTTACTTTAATGTATGTGTATGAATTATTGAGGAATCTAATTGACTATGATCTGACACTTGTGATGATAAATAATTTTGTGATATAGATGTCATCAGAGGGTCCAAACCACAACGAGTTTGATTTTGAATTTTTAGGCAACACAACAGGTGAACCTTATTCTGTGCAAACAAATGTGTATGTGAATGGAATTGGTAACCGTGAGCAAAGACTTAACCTTTGGTTTGACCCTACCAAGGATTTTCACTCTTACTCTATTTTCTGGAATCAACGTCAAGTTGTGTAAGCACCTTATTACACTCTCACTCTAATAATATTATGTGTAACATTATTTATTTTATGCTAAATTTTGTTCTTCACATAGTACTAACATGGTGCATTACTTGCAAATTTTTATATTTCTTAATACATTTATAACATATATTTAGCACATTTATAACATAATTAGCAAACAATTGTTAATAAAGTTAAGTGAAATAACTTTAGTTAACTTATAGCATATTTATAACATAGTTAACTTGTAAATTTTTCACACTTTTTATTTTTTTTTTAAAAAGTATGAAATAACTTTATATGATACTCAATAGTAATATTTTTTTTCCTTGTCAAAAAGTAATATTTTTTTTAATGAAAGAAATAAGTAGTAATTATTATGTTTTCTACTTTAGTATTATATATGTAAATTTAGTGAAGTTTAATAACAAAAGTGAAGAGAAAGTGAAGAGAATATTTTTCATAAAAAAAGAAAGGTGAAGAGAATAAAATATAAGAAAGGGAATAATGAGGTTGGATGAGTCTGAAAAGGTTGTAAAAGGAAACAAAAATGCTTTATTTTTCTCTAATGGTGGTTGAGCCATCATAGCCTTTGATTGTCATTGTGTTCCCCCGTGGGTCACTCAAATACAAATCAATGGAAAATAAAAGGTGATGGAATCTTGTACAATTTATGTCAATACTCCTAATTGGTTAATTGCAGAATACGTTTGTACAGACACTAAACTATGTCCTTTGTAATTTCTGCAAAAATTTTTGGACATTTTAGCAGCATGCCATGCGCAACATTTTTTAACCTTTATCATAAGGGACCACTCAATCAACAGTAAAAAAATTAAAAGAAAATATGTTCATTAGTAAAAATATTTTTTTGAGTGTTTCTAACATGTGTGCTTGGACTTAGACATTTATTAAGAAATAACTAGAATAATTTTGTTCTTGAAAGCAATAATTTTTAAAATTTTCAAGAATGTGAATACCCAATGTCCTAAAGGAACATGTTAGTTTGCCATATTTTCTTGCCCCAATATTTTAATATATTCAATGATTGGTCATTTTTAATTTAGTCTATTCTTAAATTGTAATAATTTTTTTTTTCATTTTAACCTTAGCAATTTAATTTAGGCTTTCTAAAATTTAGGATGCCCATAAGTTGGGTACATAATTCACACTATGTTCATTGATCAATAATATAGTTTTTTTTTTTTTTATAATTATTGATGCAAATTCTAACTTTTTATTTTTTTTATTTTTGAACAATCAGATTTTTAGTGGATGAAACACCAATAAGGGTACACACAAACATGGAACATAAGGGTATACCATATCCAAAGGACCAACCAATGGGAGTATATAGTTCAATATGGAATGCAGATGATTGGGCAACACAAGGAGGAAGAGTGAAAACAGATTGGTCTCATGCACCCTTTATTGCAACCTACAAAGCCTTTGAAATCAATGCTTGTGAGTGTCCAGTTTCAGTGGCTGCAATGGATAATACAAAGAGGTGTAGTAGTAATGAAGATAAGAAATATTGGTGGGATGAACCTAATTTTTCAGTGCTTAATTTGCATCAAAGTCATCAACTTATGTGGGTTAAGAATCATCATATGGTTTATGATTATTGTAGTGATGGTTCTAGGTTTCCTATTACTCCTGTTGAGTGTGTCCATCATCGTCATACATAG
Protein sequence

>MS.gene071128.t1

MSSKMCWFFGLFVCLMLSVSLSTSSKFDELFQPSWAFDHFLHEGDLLKLKLDNSSGAGFVSKSKYMFGRVSIQLKLVEGDSAGTVTAFYMSSEGPNHNEFDFEFLGNTTGEPYSVQTNVYVNGIGNREQRLNLWFDPTKDFHSYSIFWNQRQVVFLVDETPIRVHTNMEHKGIPYPKDQPMGVYSSIWNADDWATQGGRVKTDWSHAPFIATYKAFEINACECPVSVAAMDNTKRCSSNEDKKYWWDEPNFSVLNLHQSHQLMWVKNHHMVYDYCSDGSRFPITPVECVHHRHT