Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene071281.t1 | XP_013447979.1 | 94.5 | 477 | 26 | 0 | 1 | 477 | 113 | 589 | 1.40E-245 | 858.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene071281.t1 | F4I9E1 | 21.9 | 462 | 304 | 13 | 14 | 467 | 153 | 565 | 6.2e-13 | 77.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene071281.t1 | A0A072TXV1 | 94.5 | 477 | 26 | 0 | 1 | 477 | 113 | 589 | 9.9e-246 | 858.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene071281 | MS.gene53779 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene071281.t1 | MTR_7g029015 | 97.904 | 477 | 10 | 0 | 1 | 477 | 113 | 589 | 0.0 | 947 |
| MS.gene071281.t1 | MTR_3g099150 | 48.548 | 482 | 201 | 5 | 1 | 470 | 113 | 559 | 2.38e-147 | 432 |
| MS.gene071281.t1 | MTR_2g450710 | 34.968 | 469 | 282 | 7 | 7 | 474 | 116 | 562 | 2.42e-86 | 276 |
| MS.gene071281.t1 | MTR_4g116210 | 34.874 | 476 | 278 | 8 | 7 | 474 | 117 | 568 | 2.02e-84 | 271 |
| MS.gene071281.t1 | MTR_5g022380 | 34.244 | 476 | 254 | 7 | 1 | 473 | 1 | 420 | 1.47e-78 | 251 |
| MS.gene071281.t1 | MTR_2g100400 | 33.405 | 467 | 246 | 10 | 1 | 464 | 124 | 528 | 8.66e-78 | 253 |
| MS.gene071281.t1 | MTR_5g022380 | 34.034 | 476 | 255 | 7 | 1 | 473 | 111 | 530 | 5.23e-77 | 251 |
| MS.gene071281.t1 | MTR_1g111510 | 34.043 | 470 | 267 | 9 | 1 | 464 | 124 | 556 | 4.11e-76 | 249 |
| MS.gene071281.t1 | MTR_1g111530 | 32.632 | 475 | 267 | 8 | 1 | 464 | 120 | 552 | 6.50e-75 | 246 |
| MS.gene071281.t1 | MTR_4g073690 | 32.008 | 478 | 262 | 5 | 1 | 468 | 113 | 537 | 6.92e-75 | 245 |
| MS.gene071281.t1 | MTR_7g102880 | 33.474 | 475 | 272 | 7 | 1 | 464 | 127 | 568 | 1.20e-74 | 246 |
| MS.gene071281.t1 | MTR_5g090570 | 33.125 | 480 | 256 | 10 | 1 | 477 | 115 | 532 | 1.75e-74 | 244 |
| MS.gene071281.t1 | MTR_1g111540 | 33.689 | 469 | 270 | 10 | 1 | 464 | 124 | 556 | 3.27e-74 | 244 |
| MS.gene071281.t1 | MTR_1g111550 | 34.244 | 476 | 257 | 13 | 1 | 464 | 123 | 554 | 2.57e-73 | 242 |
| MS.gene071281.t1 | MTR_1g050550 | 32.716 | 486 | 274 | 10 | 1 | 470 | 122 | 570 | 9.81e-72 | 238 |
| MS.gene071281.t1 | MTR_1g059080 | 33.333 | 483 | 266 | 11 | 1 | 470 | 122 | 561 | 7.48e-68 | 228 |
| MS.gene071281.t1 | MTR_2g104250 | 32.320 | 362 | 172 | 8 | 119 | 474 | 4 | 298 | 4.29e-51 | 176 |
| MS.gene071281.t1 | MTR_4g073690 | 29.653 | 317 | 178 | 4 | 1 | 307 | 113 | 394 | 2.86e-37 | 141 |
| MS.gene071281.t1 | MTR_7g083660 | 22.547 | 479 | 319 | 14 | 14 | 475 | 117 | 560 | 1.39e-20 | 95.1 |
| MS.gene071281.t1 | MTR_5g026350 | 23.326 | 463 | 322 | 11 | 14 | 464 | 117 | 558 | 2.44e-19 | 91.3 |
| MS.gene071281.t1 | MTR_3g466040 | 23.028 | 469 | 295 | 11 | 3 | 466 | 107 | 514 | 6.22e-18 | 86.7 |
| MS.gene071281.t1 | MTR_8g071920 | 26.616 | 263 | 158 | 7 | 215 | 464 | 359 | 599 | 2.05e-14 | 76.3 |
| MS.gene071281.t1 | MTR_3g466110 | 27.586 | 174 | 123 | 1 | 234 | 407 | 304 | 474 | 2.76e-12 | 69.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene071281.t1 | AT5G14120 | 69.053 | 475 | 132 | 4 | 1 | 471 | 114 | 577 | 0.0 | 647 |
| MS.gene071281.t1 | AT3G01930 | 67.500 | 480 | 136 | 4 | 1 | 471 | 114 | 582 | 0.0 | 646 |
| MS.gene071281.t1 | AT3G01930 | 67.500 | 480 | 136 | 4 | 1 | 471 | 1 | 469 | 0.0 | 646 |
| MS.gene071281.t1 | AT3G01930 | 69.255 | 322 | 90 | 3 | 1 | 313 | 114 | 435 | 3.94e-149 | 432 |
| MS.gene071281.t1 | AT5G50520 | 44.397 | 473 | 209 | 6 | 3 | 472 | 117 | 538 | 1.23e-135 | 402 |
| MS.gene071281.t1 | AT5G50630 | 44.397 | 473 | 209 | 6 | 3 | 472 | 117 | 538 | 1.23e-135 | 402 |
| MS.gene071281.t1 | AT2G39210 | 34.375 | 480 | 269 | 8 | 1 | 470 | 119 | 562 | 9.18e-84 | 270 |
| MS.gene071281.t1 | AT4G34950 | 32.712 | 483 | 280 | 7 | 1 | 474 | 114 | 560 | 1.75e-82 | 266 |
| MS.gene071281.t1 | AT1G80530 | 34.607 | 471 | 271 | 6 | 7 | 474 | 117 | 553 | 8.77e-82 | 264 |
| MS.gene071281.t1 | AT2G16660 | 33.333 | 471 | 262 | 7 | 1 | 468 | 112 | 533 | 1.55e-78 | 255 |
| MS.gene071281.t1 | AT2G28120 | 33.680 | 481 | 266 | 12 | 1 | 470 | 115 | 553 | 5.39e-76 | 249 |
| MS.gene071281.t1 | AT1G74780 | 33.120 | 468 | 243 | 8 | 1 | 464 | 121 | 522 | 8.09e-74 | 242 |
| MS.gene071281.t1 | AT2G34350 | 30.882 | 476 | 260 | 9 | 1 | 474 | 117 | 525 | 3.29e-67 | 224 |
| MS.gene071281.t1 | AT2G34350 | 30.882 | 476 | 260 | 9 | 1 | 474 | 117 | 525 | 3.29e-67 | 224 |
| MS.gene071281.t1 | AT1G18940 | 29.661 | 472 | 259 | 6 | 1 | 470 | 121 | 521 | 1.92e-66 | 223 |
| MS.gene071281.t1 | AT2G34355 | 30.625 | 480 | 258 | 10 | 1 | 474 | 113 | 523 | 2.64e-64 | 217 |
| MS.gene071281.t1 | AT2G34355 | 30.385 | 441 | 232 | 10 | 40 | 474 | 2 | 373 | 1.01e-54 | 187 |
| MS.gene071281.t1 | AT2G16660 | 32.903 | 310 | 174 | 6 | 1 | 307 | 112 | 390 | 1.84e-43 | 158 |
| MS.gene071281.t1 | AT2G30300 | 23.913 | 460 | 280 | 10 | 14 | 465 | 100 | 497 | 8.27e-21 | 95.5 |
| MS.gene071281.t1 | AT4G19450 | 25.229 | 436 | 294 | 13 | 14 | 438 | 117 | 531 | 3.96e-19 | 90.9 |
| MS.gene071281.t1 | AT3G01630 | 24.625 | 467 | 286 | 14 | 14 | 467 | 151 | 564 | 5.58e-19 | 90.1 |
| MS.gene071281.t1 | AT3G01630 | 24.625 | 467 | 286 | 14 | 14 | 467 | 133 | 546 | 8.50e-19 | 89.7 |
| MS.gene071281.t1 | AT1G31470 | 21.748 | 469 | 296 | 14 | 14 | 467 | 153 | 565 | 1.44e-16 | 82.8 |
| MS.gene071281.t1 | AT5G45275 | 27.572 | 243 | 155 | 6 | 224 | 464 | 332 | 555 | 2.08e-14 | 75.9 |
Find 113 sgRNAs with CRISPR-Local
Find 154 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGAGATTGTCCTTCTGTTTC+TGG | 0.103789 | 7.1:+68419765 | None:intergenic |
| AAATCTTTCACGTTGGTTTA+TGG | 0.174292 | 7.1:-68419975 | MS.gene071281:CDS |
| GCTACAGCCTCTGAATTATT+TGG | 0.203666 | 7.1:-68418632 | MS.gene071281:CDS |
| TCGTCTCCATGATCAGTATT+TGG | 0.218122 | 7.1:-68419419 | MS.gene071281:CDS |
| ATGGTCTTAGGTTCTGGATC+TGG | 0.228794 | 7.1:-68419507 | MS.gene071281:CDS |
| ATCAGTATTTGGAACTTTCT+TGG | 0.238824 | 7.1:-68419408 | MS.gene071281:CDS |
| CTACTTCAACACAGTTTCTT+TGG | 0.254430 | 7.1:-68420228 | MS.gene071281:CDS |
| CGGTCAGATGAGCCAGTCTT+TGG | 0.285592 | 7.1:-68419463 | MS.gene071281:CDS |
| CTTGTTGGCTGCTTATTTGA+TGG | 0.308655 | 7.1:-68419943 | MS.gene071281:CDS |
| ATTGTTTATGCTAGCTTGTA+TGG | 0.317743 | 7.1:-68418323 | MS.gene071281:CDS |
| ATTTCCATGGTCTTAGGTTC+TGG | 0.350122 | 7.1:-68419513 | MS.gene071281:CDS |
| GACTTGGTTACGGTGCTCAT+TGG | 0.360347 | 7.1:-68418667 | MS.gene071281:CDS |
| TCATGCATCGTTAATATTCA+TGG | 0.366980 | 7.1:-68420087 | MS.gene071281:CDS |
| GCAGCTAATGCAGCTGGTCT+TGG | 0.373732 | 7.1:+68418773 | None:intergenic |
| TCTCTAGTCTCATTGCTAGT+AGG | 0.379404 | 7.1:-68418544 | MS.gene071281:CDS |
| TTCAAAATTTCCCGAAAAGC+CGG | 0.380401 | 7.1:-68420196 | MS.gene071281:CDS |
| CTTATATTTGTGGGAACAAA+TGG | 0.381637 | 7.1:-68420257 | MS.gene071281:CDS |
| TGCTTATTTGATGGGAGTTA+TGG | 0.383685 | 7.1:-68419934 | MS.gene071281:CDS |
| TGGTCTTAGGTTCTGGATCT+GGG | 0.391749 | 7.1:-68419506 | MS.gene071281:CDS |
| TTATGGTGTATGTCTCTTGT+TGG | 0.401325 | 7.1:-68419958 | MS.gene071281:CDS |
| TACGTTGGCACTTTACTAGT+TGG | 0.411740 | 7.1:-68418689 | MS.gene071281:CDS |
| AATATTAACGATGCATGATC+CGG | 0.416556 | 7.1:+68420092 | None:intergenic |
| TTCATTGGCATGGGATGGCC+AGG | 0.416629 | 7.1:-68418719 | MS.gene071281:CDS |
| GAGAAAATAAGAGTTCCAAC+AGG | 0.416892 | 7.1:+68418563 | None:intergenic |
| TTACTAGTTGGACTTGGTTA+CGG | 0.418091 | 7.1:-68418677 | MS.gene071281:CDS |
| GGAAGCAAGTCCACTTCCTT+AGG | 0.419733 | 7.1:+68419696 | None:intergenic |
| AAAGTTCCAAATACTGATCA+TGG | 0.424968 | 7.1:+68419413 | None:intergenic |
| TTATTTGGTTTAAGAAACTT+TGG | 0.428476 | 7.1:-68418617 | MS.gene071281:CDS |
| ATGTGTATTCTTATATTTGT+GGG | 0.431347 | 7.1:-68420266 | MS.gene071281:CDS |
| TCAAAATTTCCCGAAAAGCC+GGG | 0.432516 | 7.1:-68420195 | MS.gene071281:CDS |
| TTGTTGGCTGCTTATTTGAT+GGG | 0.435499 | 7.1:-68419942 | MS.gene071281:CDS |
| CGTTTCTGCCTTTCCGATGC+AGG | 0.437114 | 7.1:+68419675 | None:intergenic |
| AATGACAAAGGAATTATAAT+CGG | 0.440338 | 7.1:+68419834 | None:intergenic |
| CATATGGAACCTTCGCACTT+TGG | 0.443970 | 7.1:+68418428 | None:intergenic |
| ATTCCTTTGTCATTAACCTT+CGG | 0.444957 | 7.1:-68419825 | MS.gene071281:CDS |
| TGAAGTAATATTAAGTGAGT+TGG | 0.445671 | 7.1:-68419730 | MS.gene071281:CDS |
| AAGGAGCTGTGAGGGTTAAG+AGG | 0.447202 | 7.1:-68419611 | MS.gene071281:CDS |
| ATACGAACGATGCTATATTC+AGG | 0.459391 | 7.1:+68418477 | None:intergenic |
| GGATTATGCATTGTTGCGGC+AGG | 0.460540 | 7.1:-68418377 | MS.gene071281:CDS |
| ACTTGGTTACGGTGCTCATT+GGG | 0.463235 | 7.1:-68418666 | MS.gene071281:CDS |
| CTGCCATGATAGTGGATGTC+AGG | 0.467856 | 7.1:+68418399 | None:intergenic |
| AACTTTCTTGGACGTGTTGG+AGG | 0.470215 | 7.1:-68419396 | MS.gene071281:CDS |
| GGTCAGATGAGCCAGTCTTT+GGG | 0.473244 | 7.1:-68419462 | MS.gene071281:CDS |
| GCATAATCCTGCCATGATAG+TGG | 0.474193 | 7.1:+68418391 | None:intergenic |
| CAAAGAAACTGTGTTGAAGT+AGG | 0.476111 | 7.1:+68420229 | None:intergenic |
| TAGCATAAACAATCCTTGTT+CGG | 0.485537 | 7.1:+68418333 | None:intergenic |
| AATAATTCAGAGGCTGTAGC+TGG | 0.491533 | 7.1:+68418635 | None:intergenic |
| GGTCCAGCATTGGTAGGAAT+AGG | 0.493698 | 7.1:-68420056 | MS.gene071281:CDS |
| ATGTTCATTGTTAGACCTGT+CGG | 0.494988 | 7.1:-68420029 | MS.gene071281:CDS |
| CTGCTGCAAGCTGCTGCAGA+AGG | 0.495751 | 7.1:-68419630 | MS.gene071281:CDS |
| TTAATATTCATGGTTGCTGT+TGG | 0.497023 | 7.1:-68420077 | MS.gene071281:CDS |
| AGTGGACTTGCTTCCTGCAT+CGG | 0.504812 | 7.1:-68419688 | MS.gene071281:CDS |
| AGACCTATTCCTACCAATGC+TGG | 0.505924 | 7.1:+68420053 | None:intergenic |
| GGATGTCAGGAAGAAGCATA+TGG | 0.506273 | 7.1:+68418412 | None:intergenic |
| CCCGAAAAGCCGGGGTCCCG+TGG | 0.506898 | 7.1:-68420186 | MS.gene071281:CDS |
| TTTCTTGGACGTGTTGGAGG+CGG | 0.511820 | 7.1:-68419393 | MS.gene071281:CDS |
| TGGAACTTTCTTGGACGTGT+TGG | 0.513657 | 7.1:-68419399 | MS.gene071281:CDS |
| ACTTGTTTGTGACCTCCGAC+AGG | 0.514273 | 7.1:+68420014 | None:intergenic |
| AAAAGCCGGGGTCCCGTGGT+TGG | 0.515273 | 7.1:-68420182 | MS.gene071281:CDS |
| AGATGAGAAGCCTAAGGAAG+TGG | 0.515527 | 7.1:-68419706 | MS.gene071281:CDS |
| TGTTTCTCGTGTTCCGAACA+AGG | 0.516891 | 7.1:-68418346 | MS.gene071281:CDS |
| ACGATGCATGATCCGGGGAA+TGG | 0.519353 | 7.1:+68420099 | None:intergenic |
| GTATCTAGTGCGCTTAATGT+TGG | 0.520420 | 7.1:-68418458 | MS.gene071281:CDS |
| CCCTTTAAAATTCCAACCAC+GGG | 0.526339 | 7.1:+68420170 | None:intergenic |
| CTTCCTGACATCCACTATCA+TGG | 0.528499 | 7.1:-68418402 | MS.gene071281:CDS |
| ATCACTCTAGCAAACCCTGT+TGG | 0.532002 | 7.1:-68418578 | MS.gene071281:CDS |
| GGTTGCTGTTGGTCCAGCAT+TGG | 0.533114 | 7.1:-68420066 | MS.gene071281:CDS |
| AAACAAGTGCGGCCGTCGGA+TGG | 0.533967 | 7.1:-68419999 | MS.gene071281:CDS |
| ATATTAACGATGCATGATCC+GGG | 0.537574 | 7.1:+68420093 | None:intergenic |
| GCTGTTGGTCCAGCATTGGT+AGG | 0.539156 | 7.1:-68420062 | MS.gene071281:CDS |
| GGCACTTTACTAGTTGGACT+TGG | 0.541785 | 7.1:-68418683 | MS.gene071281:CDS |
| CATTGTCATATCCCAAAGAC+TGG | 0.548663 | 7.1:+68419451 | None:intergenic |
| TGAAATGCAGCTAATGCAGC+TGG | 0.548805 | 7.1:+68418767 | None:intergenic |
| AGAAGTGCTTCTTCCTCTAG+AGG | 0.550119 | 7.1:+68419789 | None:intergenic |
| TGCTGCAGAAGGAGCTGTGA+GGG | 0.559996 | 7.1:-68419619 | MS.gene071281:CDS |
| AAGGGCTTTGCTGGATTGAG+TGG | 0.565910 | 7.1:-68420152 | MS.gene071281:CDS |
| CTGACATCCACTATCATGGC+AGG | 0.565999 | 7.1:-68418398 | MS.gene071281:CDS |
| ACGATGCTATATTCAGGTGT+TGG | 0.567259 | 7.1:+68418483 | None:intergenic |
| GAGCTGTGAGGGTTAAGAGG+AGG | 0.567528 | 7.1:-68419608 | MS.gene071281:CDS |
| CAAAATTTCCCGAAAAGCCG+GGG | 0.569217 | 7.1:-68420194 | MS.gene071281:CDS |
| AAATTCCAACCACGGGACCC+CGG | 0.570142 | 7.1:+68420177 | None:intergenic |
| GGCAGGATTATGCATTGTTG+CGG | 0.574036 | 7.1:-68418381 | MS.gene071281:CDS |
| TTTCAACTAGCTATGACTGT+TGG | 0.574245 | 7.1:-68418749 | MS.gene071281:CDS |
| GTTGGAAGATGAGAAGCCTA+AGG | 0.577142 | 7.1:-68419712 | MS.gene071281:CDS |
| GTCATTAACCTTCGGCCCTG+CGG | 0.579108 | 7.1:-68419817 | MS.gene071281:CDS |
| ATATGCAGTTATCCATTCCC+CGG | 0.581249 | 7.1:-68420111 | MS.gene071281:CDS |
| GTGAGGGTTAAGAGGAGGAG+AGG | 0.584228 | 7.1:-68419603 | MS.gene071281:CDS |
| TCTTAAACCAAATAATTCAG+AGG | 0.585066 | 7.1:+68418625 | None:intergenic |
| GCCCTTTAAAATTCCAACCA+CGG | 0.585494 | 7.1:+68420169 | None:intergenic |
| GAGCAATGAGGATCAGAAAG+AGG | 0.588717 | 7.1:+68419856 | None:intergenic |
| GTAAAATCTTCTCCTCTATG+TGG | 0.590594 | 7.1:+68419579 | None:intergenic |
| AGATCCAGAACCTAAGACCA+TGG | 0.600742 | 7.1:+68419509 | None:intergenic |
| GTGCCAACGTACATTGAACC+TGG | 0.605966 | 7.1:+68418701 | None:intergenic |
| AGGAGGAGAGGACCACATAG+AGG | 0.606447 | 7.1:-68419591 | MS.gene071281:CDS |
| TTGACTGTAATTGACAATCT+CGG | 0.606676 | 7.1:-68419483 | MS.gene071281:CDS |
| ACTTGCTTCCTGCATCGGAA+AGG | 0.615485 | 7.1:-68419683 | MS.gene071281:CDS |
| GATGTGTATTCTTATATTTG+TGG | 0.622481 | 7.1:-68420267 | MS.gene071281:CDS |
| GATTATGCATTGTTGCGGCA+GGG | 0.630180 | 7.1:-68418376 | MS.gene071281:CDS |
| GGGCCGAAGGTTAATGACAA+AGG | 0.631796 | 7.1:+68419822 | None:intergenic |
| AGAAAATAAGAGTTCCAACA+GGG | 0.638422 | 7.1:+68418564 | None:intergenic |
| TTTAATCAAAGCTTGCGTCA+AGG | 0.639178 | 7.1:+68419557 | None:intergenic |
| GAAGCAGAGAAACAAGCTCA+TGG | 0.639621 | 7.1:-68418509 | MS.gene071281:CDS |
| TCACAAACAAGTGCGGCCGT+CGG | 0.640280 | 7.1:-68420003 | MS.gene071281:CDS |
| GCACTTCTTCCAGAAACAGA+AGG | 0.653183 | 7.1:-68419774 | MS.gene071281:CDS |
| GGTGATGCGCCAAAGTGCGA+AGG | 0.660090 | 7.1:-68418437 | MS.gene071281:CDS |
| TTCATTGTTAGACCTGTCGG+AGG | 0.667965 | 7.1:-68420026 | MS.gene071281:CDS |
| TATTAACGATGCATGATCCG+GGG | 0.678287 | 7.1:+68420094 | None:intergenic |
| GGATGGAAAATCTTTCACGT+TGG | 0.680385 | 7.1:-68419982 | MS.gene071281:CDS |
| GAATTATAATCGGAGCAATG+AGG | 0.685023 | 7.1:+68419844 | None:intergenic |
| CTGCTGCAGAAGGAGCTGTG+AGG | 0.702176 | 7.1:-68419620 | MS.gene071281:CDS |
| TCGGAGGTCACAAACAAGTG+CGG | 0.705441 | 7.1:-68420010 | MS.gene071281:CDS |
| TGGCCAGGTTCAATGTACGT+TGG | 0.706240 | 7.1:-68418704 | MS.gene071281:CDS |
| GCAGAGAAACAAGCTCATGG+TGG | 0.726436 | 7.1:-68418506 | MS.gene071281:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAGCTATAAATATTTAATCA+GGG | + | chr7.1:68419239-68419258 | None:intergenic | 15.0% |
| !!! | ATTATTTTCACTTTTTTTCT+TGG | + | chr7.1:68419403-68419422 | None:intergenic | 15.0% |
| !!! | TTAAATATTTATAGCTTGTT+TGG | - | chr7.1:68419242-68419261 | MS.gene071281:intron | 15.0% |
| !! | AATGACAAAGGAATTATAAT+CGG | + | chr7.1:68418730-68418749 | None:intergenic | 20.0% |
| !! | ATGTGTATTCTTATATTTGT+GGG | - | chr7.1:68418295-68418314 | MS.gene071281:CDS | 20.0% |
| !! | CAAGCTATAAATATTTAATC+AGG | + | chr7.1:68419240-68419259 | None:intergenic | 20.0% |
| !!! | TTATTTGGTTTAAGAAACTT+TGG | - | chr7.1:68419944-68419963 | MS.gene071281:CDS | 20.0% |
| ! | AATTAAGCTGTTTATCCAAA+AGG | - | chr7.1:68419691-68419710 | MS.gene071281:CDS | 25.0% |
| ! | TCTTAAACCAAATAATTCAG+AGG | + | chr7.1:68419939-68419958 | None:intergenic | 25.0% |
| ! | TGAAGTAATATTAAGTGAGT+TGG | - | chr7.1:68418831-68418850 | MS.gene071281:intron | 25.0% |
| ! | TGGTTTACTATATAGAACTT+CGG | + | chr7.1:68419330-68419349 | None:intergenic | 25.0% |
| !! | CAATACAAAGAAAGTTTTCA+TGG | + | chr7.1:68419350-68419369 | None:intergenic | 25.0% |
| !! | CTTTGATTAAAGCAGATTTT+TGG | - | chr7.1:68419016-68419035 | MS.gene071281:intron | 25.0% |
| AAAGTTCCAAATACTGATCA+TGG | + | chr7.1:68419151-68419170 | None:intergenic | 30.0% | |
| AATATTAACGATGCATGATC+CGG | + | chr7.1:68418472-68418491 | None:intergenic | 30.0% | |
| AGAAAATAAGAGTTCCAACA+GGG | + | chr7.1:68420000-68420019 | None:intergenic | 30.0% | |
| ATTCCTTTGTCATTAACCTT+CGG | - | chr7.1:68418736-68418755 | MS.gene071281:CDS | 30.0% | |
| CTTATATTTGTGGGAACAAA+TGG | - | chr7.1:68418304-68418323 | MS.gene071281:CDS | 30.0% | |
| TAGCATAAACAATCCTTGTT+CGG | + | chr7.1:68420231-68420250 | None:intergenic | 30.0% | |
| TCATGCATCGTTAATATTCA+TGG | - | chr7.1:68418474-68418493 | MS.gene071281:CDS | 30.0% | |
| TTAATATTCATGGTTGCTGT+TGG | - | chr7.1:68418484-68418503 | MS.gene071281:CDS | 30.0% | |
| TTGACTGTAATTGACAATCT+CGG | - | chr7.1:68419078-68419097 | MS.gene071281:intron | 30.0% | |
| ! | ATCAGTATTTGGAACTTTCT+TGG | - | chr7.1:68419153-68419172 | MS.gene071281:intron | 30.0% |
| ! | ATTGTTTATGCTAGCTTGTA+TGG | - | chr7.1:68420238-68420257 | MS.gene071281:CDS | 30.0% |
| ! | TTGACAAGATTATCACCTTT+TGG | + | chr7.1:68419709-68419728 | None:intergenic | 30.0% |
| !! | AAATCTTTCACGTTGGTTTA+TGG | - | chr7.1:68418586-68418605 | MS.gene071281:CDS | 30.0% |
| !! | CTTGGTTCAAAGTTTTGTAT+AGG | + | chr7.1:68419385-68419404 | None:intergenic | 30.0% |
| AAACACTTGTGAGGTTGTTT+GGG | - | chr7.1:68419508-68419527 | MS.gene071281:CDS | 35.0% | |
| ATACGAACGATGCTATATTC+AGG | + | chr7.1:68420087-68420106 | None:intergenic | 35.0% | |
| ATATTAACGATGCATGATCC+GGG | + | chr7.1:68418471-68418490 | None:intergenic | 35.0% | |
| ATGTTCATTGTTAGACCTGT+CGG | - | chr7.1:68418532-68418551 | MS.gene071281:CDS | 35.0% | |
| CAAAGAAACTGTGTTGAAGT+AGG | + | chr7.1:68418335-68418354 | None:intergenic | 35.0% | |
| CTACTTCAACACAGTTTCTT+TGG | - | chr7.1:68418333-68418352 | MS.gene071281:CDS | 35.0% | |
| CTATATAGAACTTCGGAAAC+AGG | + | chr7.1:68419323-68419342 | None:intergenic | 35.0% | |
| CTTATTTCCTTAAGCTCTCT+AGG | - | chr7.1:68419576-68419595 | MS.gene071281:CDS | 35.0% | |
| GAATTATAATCGGAGCAATG+AGG | + | chr7.1:68418720-68418739 | None:intergenic | 35.0% | |
| GAGAAAATAAGAGTTCCAAC+AGG | + | chr7.1:68420001-68420020 | None:intergenic | 35.0% | |
| GTAAAATCTTCTCCTCTATG+TGG | + | chr7.1:68418985-68419004 | None:intergenic | 35.0% | |
| TAAACACTTGTGAGGTTGTT+TGG | - | chr7.1:68419507-68419526 | MS.gene071281:CDS | 35.0% | |
| TGCTTATTTGATGGGAGTTA+TGG | - | chr7.1:68418627-68418646 | MS.gene071281:CDS | 35.0% | |
| TTATGGTGTATGTCTCTTGT+TGG | - | chr7.1:68418603-68418622 | MS.gene071281:CDS | 35.0% | |
| TTCAAAATTTCCCGAAAAGC+CGG | - | chr7.1:68418365-68418384 | MS.gene071281:CDS | 35.0% | |
| TTTAATCAAAGCTTGCGTCA+AGG | + | chr7.1:68419007-68419026 | None:intergenic | 35.0% | |
| TTTCAACTAGCTATGACTGT+TGG | - | chr7.1:68419812-68419831 | MS.gene071281:CDS | 35.0% | |
| ! | ACTGTTGGTCATCTTTTCAT+TGG | - | chr7.1:68419827-68419846 | MS.gene071281:CDS | 35.0% |
| ! | CTTTTCATTTCCATGGTCTT+AGG | - | chr7.1:68419042-68419061 | MS.gene071281:intron | 35.0% |
| ! | TTACTAGTTGGACTTGGTTA+CGG | - | chr7.1:68419884-68419903 | MS.gene071281:CDS | 35.0% |
| ! | TTGGCTTCTTTTCATTTCCA+TGG | - | chr7.1:68419035-68419054 | MS.gene071281:intron | 35.0% |
| ! | TTGTTGGCTGCTTATTTGAT+GGG | - | chr7.1:68418619-68418638 | MS.gene071281:CDS | 35.0% |
| AATAATTCAGAGGCTGTAGC+TGG | + | chr7.1:68419929-68419948 | None:intergenic | 40.0% | |
| ACGATGCTATATTCAGGTGT+TGG | + | chr7.1:68420081-68420100 | None:intergenic | 40.0% | |
| AGAAAATCGATTGAGCCCTT+AGG | - | chr7.1:68419456-68419475 | MS.gene071281:CDS | 40.0% | |
| AGCTCAAATAAGCCAACCTA+AGG | + | chr7.1:68419475-68419494 | None:intergenic | 40.0% | |
| ATATGCAGTTATCCATTCCC+CGG | - | chr7.1:68418450-68418469 | MS.gene071281:CDS | 40.0% | |
| ATTTCCTTAAGCTCTCTAGG+AGG | - | chr7.1:68419579-68419598 | MS.gene071281:CDS | 40.0% | |
| CATTGTCATATCCCAAAGAC+TGG | + | chr7.1:68419113-68419132 | None:intergenic | 40.0% | |
| GCTACAGCCTCTGAATTATT+TGG | - | chr7.1:68419929-68419948 | MS.gene071281:CDS | 40.0% | |
| GCTCAAATAAGCCAACCTAA+GGG | + | chr7.1:68419474-68419493 | None:intergenic | 40.0% | |
| GGATGGAAAATCTTTCACGT+TGG | - | chr7.1:68418579-68418598 | MS.gene071281:CDS | 40.0% | |
| GTATCTAGTGCGCTTAATGT+TGG | - | chr7.1:68420103-68420122 | MS.gene071281:CDS | 40.0% | |
| TACGAGCATAAACACTTGTG+AGG | - | chr7.1:68419499-68419518 | MS.gene071281:CDS | 40.0% | |
| TACGTTGGCACTTTACTAGT+TGG | - | chr7.1:68419872-68419891 | MS.gene071281:CDS | 40.0% | |
| TATTAACGATGCATGATCCG+GGG | + | chr7.1:68418470-68418489 | None:intergenic | 40.0% | |
| TCAAAATTTCCCGAAAAGCC+GGG | - | chr7.1:68418366-68418385 | MS.gene071281:CDS | 40.0% | |
| TCGTCTCCATGATCAGTATT+TGG | - | chr7.1:68419142-68419161 | MS.gene071281:intron | 40.0% | |
| TCTCTAGTCTCATTGCTAGT+AGG | - | chr7.1:68420017-68420036 | MS.gene071281:CDS | 40.0% | |
| TGAGATTGTCCTTCTGTTTC+TGG | + | chr7.1:68418799-68418818 | None:intergenic | 40.0% | |
| ! | ATCTTTTCATTGGCATGGGA+TGG | - | chr7.1:68419837-68419856 | MS.gene071281:CDS | 40.0% |
| ! | ATTTCCATGGTCTTAGGTTC+TGG | - | chr7.1:68419048-68419067 | MS.gene071281:intron | 40.0% |
| ! | CCCGTGGTTGGAATTTTAAA+GGG | - | chr7.1:68418391-68418410 | MS.gene071281:CDS | 40.0% |
| ! | CTTGTTGGCTGCTTATTTGA+TGG | - | chr7.1:68418618-68418637 | MS.gene071281:CDS | 40.0% |
| ! | GGTCATCTTTTCATTGGCAT+GGG | - | chr7.1:68419833-68419852 | MS.gene071281:CDS | 40.0% |
| ! | GTGAAAGATTTTCCATCCGA+CGG | + | chr7.1:68418577-68418596 | None:intergenic | 40.0% |
| ! | TCCCGTGGTTGGAATTTTAA+AGG | - | chr7.1:68418390-68418409 | MS.gene071281:CDS | 40.0% |
| ! | TGGTCATCTTTTCATTGGCA+TGG | - | chr7.1:68419832-68419851 | MS.gene071281:CDS | 40.0% |
| ! | TTTCCTTAAGCTCTCTAGGA+GGG | - | chr7.1:68419580-68419599 | MS.gene071281:CDS | 40.0% |
| !! | CCCTTTAAAATTCCAACCAC+GGG | + | chr7.1:68418394-68418413 | None:intergenic | 40.0% |
| !! | GCCCTTTAAAATTCCAACCA+CGG | + | chr7.1:68418395-68418414 | None:intergenic | 40.0% |
| !! | GGAATTTTAAAGGGCTTTGC+TGG | - | chr7.1:68418400-68418419 | MS.gene071281:CDS | 40.0% |
| AACTTTCTTGGACGTGTTGG+AGG | - | chr7.1:68419165-68419184 | MS.gene071281:intron | 45.0% | |
| AATCGATTGAGCCCTTAGGT+TGG | - | chr7.1:68419460-68419479 | MS.gene071281:CDS | 45.0% | |
| ACTTGGTTACGGTGCTCATT+GGG | - | chr7.1:68419895-68419914 | MS.gene071281:CDS | 45.0% | |
| AGACCTATTCCTACCAATGC+TGG | + | chr7.1:68418511-68418530 | None:intergenic | 45.0% | |
| AGATCCAGAACCTAAGACCA+TGG | + | chr7.1:68419055-68419074 | None:intergenic | 45.0% | |
| AGATGAGAAGCCTAAGGAAG+TGG | - | chr7.1:68418855-68418874 | MS.gene071281:intron | 45.0% | |
| ATCACTCTAGCAAACCCTGT+TGG | - | chr7.1:68419983-68420002 | MS.gene071281:CDS | 45.0% | |
| CAAAATTTCCCGAAAAGCCG+GGG | - | chr7.1:68418367-68418386 | MS.gene071281:CDS | 45.0% | |
| CATATGGAACCTTCGCACTT+TGG | + | chr7.1:68420136-68420155 | None:intergenic | 45.0% | |
| CCCTGCTCAGTAAAACACAT+CGG | + | chr7.1:68419757-68419776 | None:intergenic | 45.0% | |
| CTTCCTGACATCCACTATCA+TGG | - | chr7.1:68420159-68420178 | MS.gene071281:CDS | 45.0% | |
| GAAGCAGAGAAACAAGCTCA+TGG | - | chr7.1:68420052-68420071 | MS.gene071281:CDS | 45.0% | |
| GAGCAATGAGGATCAGAAAG+AGG | + | chr7.1:68418708-68418727 | None:intergenic | 45.0% | |
| GATTATGCATTGTTGCGGCA+GGG | - | chr7.1:68420185-68420204 | MS.gene071281:CDS | 45.0% | |
| GCACTTCTTCCAGAAACAGA+AGG | - | chr7.1:68418787-68418806 | MS.gene071281:CDS | 45.0% | |
| GCATAATCCTGCCATGATAG+TGG | + | chr7.1:68420173-68420192 | None:intergenic | 45.0% | |
| GGCACTTTACTAGTTGGACT+TGG | - | chr7.1:68419878-68419897 | MS.gene071281:CDS | 45.0% | |
| GGCAGGATTATGCATTGTTG+CGG | - | chr7.1:68420180-68420199 | MS.gene071281:CDS | 45.0% | |
| GTTGGAAGATGAGAAGCCTA+AGG | - | chr7.1:68418849-68418868 | MS.gene071281:intron | 45.0% | |
| TGAAATGCAGCTAATGCAGC+TGG | + | chr7.1:68419797-68419816 | None:intergenic | 45.0% | |
| TGGAACTTTCTTGGACGTGT+TGG | - | chr7.1:68419162-68419181 | MS.gene071281:intron | 45.0% | |
| TGTTTCTCGTGTTCCGAACA+AGG | - | chr7.1:68420215-68420234 | MS.gene071281:CDS | 45.0% | |
| TTCATTGTTAGACCTGTCGG+AGG | - | chr7.1:68418535-68418554 | MS.gene071281:CDS | 45.0% | |
| ! | CCGATGTGTTTTACTGAGCA+GGG | - | chr7.1:68419754-68419773 | MS.gene071281:CDS | 45.0% |
| ! | GGATGTCAGGAAGAAGCATA+TGG | + | chr7.1:68420152-68420171 | None:intergenic | 45.0% |
| ! | TCCGATGTGTTTTACTGAGC+AGG | - | chr7.1:68419753-68419772 | MS.gene071281:CDS | 45.0% |
| !! | AGAAGTGCTTCTTCCTCTAG+AGG | + | chr7.1:68418775-68418794 | None:intergenic | 45.0% |
| !! | ATGGTCTTAGGTTCTGGATC+TGG | - | chr7.1:68419054-68419073 | MS.gene071281:intron | 45.0% |
| !! | TGGTCTTAGGTTCTGGATCT+GGG | - | chr7.1:68419055-68419074 | MS.gene071281:intron | 45.0% |
| !!! | AAAAATAACTTTTTTAATAT+AGG | + | chr7.1:68419626-68419645 | None:intergenic | 5.0% |
| AAGCCCTCCTAGAGAGCTTA+AGG | + | chr7.1:68419586-68419605 | None:intergenic | 50.0% | |
| AAGGAGCTGTGAGGGTTAAG+AGG | - | chr7.1:68418950-68418969 | MS.gene071281:intron | 50.0% | |
| AAGGGCTTTGCTGGATTGAG+TGG | - | chr7.1:68418409-68418428 | MS.gene071281:CDS | 50.0% | |
| ACTTGCTTCCTGCATCGGAA+AGG | - | chr7.1:68418878-68418897 | MS.gene071281:intron | 50.0% | |
| ACTTGTTTGTGACCTCCGAC+AGG | + | chr7.1:68418550-68418569 | None:intergenic | 50.0% | |
| CTGACATCCACTATCATGGC+AGG | - | chr7.1:68420163-68420182 | MS.gene071281:CDS | 50.0% | |
| GCAGAGAAACAAGCTCATGG+TGG | - | chr7.1:68420055-68420074 | MS.gene071281:CDS | 50.0% | |
| GGAAGCAAGTCCACTTCCTT+AGG | + | chr7.1:68418868-68418887 | None:intergenic | 50.0% | |
| GGATTATGCATTGTTGCGGC+AGG | - | chr7.1:68420184-68420203 | MS.gene071281:CDS | 50.0% | |
| GGGCCGAAGGTTAATGACAA+AGG | + | chr7.1:68418742-68418761 | None:intergenic | 50.0% | |
| GGTCAGATGAGCCAGTCTTT+GGG | - | chr7.1:68419099-68419118 | MS.gene071281:intron | 50.0% | |
| GTGCCAACGTACATTGAACC+TGG | + | chr7.1:68419863-68419882 | None:intergenic | 50.0% | |
| TCGGAGGTCACAAACAAGTG+CGG | - | chr7.1:68418551-68418570 | MS.gene071281:CDS | 50.0% | |
| TGGCCAGGTTCAATGTACGT+TGG | - | chr7.1:68419857-68419876 | MS.gene071281:CDS | 50.0% | |
| TTTCTTGGACGTGTTGGAGG+CGG | - | chr7.1:68419168-68419187 | MS.gene071281:intron | 50.0% | |
| ! | AGTGGACTTGCTTCCTGCAT+CGG | - | chr7.1:68418873-68418892 | MS.gene071281:intron | 50.0% |
| ! | GACTTGGTTACGGTGCTCAT+TGG | - | chr7.1:68419894-68419913 | MS.gene071281:CDS | 50.0% |
| !! | CCTCTAGAGGTTTTTCCGCA+GGG | + | chr7.1:68418762-68418781 | None:intergenic | 50.0% |
| !! | CTGCCATGATAGTGGATGTC+AGG | + | chr7.1:68420165-68420184 | None:intergenic | 50.0% |
| !! | GGTCCAGCATTGGTAGGAAT+AGG | - | chr7.1:68418505-68418524 | MS.gene071281:CDS | 50.0% |
| !! | TCCTCTAGAGGTTTTTCCGC+AGG | + | chr7.1:68418763-68418782 | None:intergenic | 50.0% |
| AAATTCCAACCACGGGACCC+CGG | + | chr7.1:68418387-68418406 | None:intergenic | 55.0% | |
| ACGATGCATGATCCGGGGAA+TGG | + | chr7.1:68418465-68418484 | None:intergenic | 55.0% | |
| AGGAGGAGAGGACCACATAG+AGG | - | chr7.1:68418970-68418989 | MS.gene071281:intron | 55.0% | |
| CCCTGCGGAAAAACCTCTAG+AGG | - | chr7.1:68418759-68418778 | MS.gene071281:CDS | 55.0% | |
| CGGTCAGATGAGCCAGTCTT+TGG | - | chr7.1:68419098-68419117 | MS.gene071281:intron | 55.0% | |
| CGTTTCTGCCTTTCCGATGC+AGG | + | chr7.1:68418889-68418908 | None:intergenic | 55.0% | |
| GAGCTGTGAGGGTTAAGAGG+AGG | - | chr7.1:68418953-68418972 | MS.gene071281:intron | 55.0% | |
| GTCATTAACCTTCGGCCCTG+CGG | - | chr7.1:68418744-68418763 | MS.gene071281:CDS | 55.0% | |
| GTGAGGGTTAAGAGGAGGAG+AGG | - | chr7.1:68418958-68418977 | MS.gene071281:intron | 55.0% | |
| TCACAAACAAGTGCGGCCGT+CGG | - | chr7.1:68418558-68418577 | MS.gene071281:CDS | 55.0% | |
| TGCTGCAGAAGGAGCTGTGA+GGG | - | chr7.1:68418942-68418961 | MS.gene071281:intron | 55.0% | |
| TTCATTGGCATGGGATGGCC+AGG | - | chr7.1:68419842-68419861 | MS.gene071281:CDS | 55.0% | |
| ! | GCAGCTAATGCAGCTGGTCT+TGG | + | chr7.1:68419791-68419810 | None:intergenic | 55.0% |
| ! | GGTTGCTGTTGGTCCAGCAT+TGG | - | chr7.1:68418495-68418514 | MS.gene071281:CDS | 55.0% |
| !! | GCTGTTGGTCCAGCATTGGT+AGG | - | chr7.1:68418499-68418518 | MS.gene071281:CDS | 55.0% |
| AAACAAGTGCGGCCGTCGGA+TGG | - | chr7.1:68418562-68418581 | MS.gene071281:CDS | 60.0% | |
| CTGCTGCAAGCTGCTGCAGA+AGG | - | chr7.1:68418931-68418950 | MS.gene071281:intron | 60.0% | |
| CTGCTGCAGAAGGAGCTGTG+AGG | - | chr7.1:68418941-68418960 | MS.gene071281:intron | 60.0% | |
| GGTGATGCGCCAAAGTGCGA+AGG | - | chr7.1:68420124-68420143 | MS.gene071281:CDS | 60.0% | |
| ! | AGGTTTTTCCGCAGGGCCGA+AGG | + | chr7.1:68418755-68418774 | None:intergenic | 60.0% |
| AAAAGCCGGGGTCCCGTGGT+TGG | - | chr7.1:68418379-68418398 | MS.gene071281:CDS | 65.0% | |
| ! | ACCACGGGACCCCGGCTTTT+CGG | + | chr7.1:68418379-68418398 | None:intergenic | 65.0% |
| ! | CCACGGGACCCCGGCTTTTC+GGG | + | chr7.1:68418378-68418397 | None:intergenic | 70.0% |
| CCCGAAAAGCCGGGGTCCCG+TGG | - | chr7.1:68418375-68418394 | MS.gene071281:CDS | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.1 | gene | 68418295 | 68420288 | 68418295 | ID=MS.gene071281 |
| chr7.1 | mRNA | 68418295 | 68420288 | 68418295 | ID=MS.gene071281.t1;Parent=MS.gene071281 |
| chr7.1 | exon | 68419369 | 68420288 | 68419369 | ID=MS.gene071281.t1.exon1;Parent=MS.gene071281.t1 |
| chr7.1 | CDS | 68419369 | 68420288 | 68419369 | ID=cds.MS.gene071281.t1;Parent=MS.gene071281.t1 |
| chr7.1 | exon | 68418295 | 68418808 | 68418295 | ID=MS.gene071281.t1.exon2;Parent=MS.gene071281.t1 |
| chr7.1 | CDS | 68418295 | 68418808 | 68418295 | ID=cds.MS.gene071281.t1;Parent=MS.gene071281.t1 |
| Gene Sequence |
| Protein sequence |
