Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene071381.t1 | XP_013446985.1 | 94.1 | 269 | 15 | 1 | 54 | 322 | 54 | 321 | 1.50E-81 | 313.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene071381.t1 | Q9C5D3 | 82.3 | 164 | 27 | 1 | 54 | 217 | 76 | 237 | 9.0e-48 | 192.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene071381.t1 | A0A072TUA8 | 94.1 | 269 | 15 | 1 | 54 | 322 | 54 | 321 | 1.0e-81 | 313.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene05894 | MS.gene071381 | 0.809666 | 1.69E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene071381.t1 | MTR_8g096700 | 93.455 | 275 | 17 | 1 | 48 | 322 | 48 | 321 | 1.04e-144 | 410 |
| MS.gene071381.t1 | MTR_3g099030 | 53.137 | 271 | 88 | 6 | 52 | 322 | 35 | 266 | 1.32e-78 | 241 |
| MS.gene071381.t1 | MTR_3g099040 | 51.042 | 288 | 104 | 4 | 52 | 322 | 35 | 302 | 1.57e-75 | 234 |
| MS.gene071381.t1 | MTR_2g026685 | 49.084 | 273 | 104 | 5 | 50 | 322 | 32 | 269 | 1.81e-55 | 181 |
| MS.gene071381.t1 | MTR_2g026685 | 49.084 | 273 | 104 | 5 | 50 | 322 | 33 | 270 | 1.86e-55 | 181 |
| MS.gene071381.t1 | MTR_2g026685 | 47.727 | 264 | 103 | 5 | 59 | 322 | 1 | 229 | 4.70e-51 | 169 |
| MS.gene071381.t1 | MTR_4g006650 | 62.275 | 167 | 53 | 3 | 10 | 176 | 133 | 289 | 4.82e-42 | 148 |
| MS.gene071381.t1 | MTR_3g435930 | 35.897 | 273 | 109 | 6 | 53 | 322 | 28 | 237 | 2.43e-41 | 144 |
| MS.gene071381.t1 | MTR_3g093020 | 35.048 | 311 | 161 | 9 | 19 | 321 | 6 | 283 | 2.50e-32 | 122 |
| MS.gene071381.t1 | MTR_4g057765 | 30.686 | 277 | 163 | 5 | 54 | 322 | 69 | 324 | 1.45e-30 | 118 |
| MS.gene071381.t1 | MTR_1g063210 | 31.742 | 356 | 159 | 11 | 26 | 322 | 3 | 333 | 4.42e-29 | 114 |
| MS.gene071381.t1 | MTR_5g069180 | 36.810 | 163 | 91 | 2 | 54 | 216 | 56 | 206 | 2.94e-28 | 112 |
| MS.gene071381.t1 | MTR_8g010540 | 34.386 | 285 | 149 | 11 | 37 | 321 | 18 | 264 | 3.94e-27 | 107 |
| MS.gene071381.t1 | MTR_2g095480 | 34.731 | 167 | 104 | 3 | 50 | 216 | 64 | 225 | 9.55e-25 | 102 |
| MS.gene071381.t1 | MTR_0041s0140 | 33.161 | 193 | 109 | 3 | 43 | 217 | 5 | 195 | 4.70e-21 | 90.9 |
| MS.gene071381.t1 | MTR_7g101930 | 33.161 | 193 | 109 | 3 | 43 | 217 | 5 | 195 | 6.78e-21 | 92.0 |
| MS.gene071381.t1 | MTR_8g018747 | 50.000 | 62 | 31 | 0 | 155 | 216 | 23 | 84 | 1.38e-14 | 72.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene071381.t1 | AT5G50740 | 52.593 | 270 | 113 | 4 | 53 | 322 | 29 | 283 | 1.52e-83 | 254 |
| MS.gene071381.t1 | AT5G50740 | 52.593 | 270 | 113 | 4 | 53 | 322 | 29 | 283 | 1.52e-83 | 254 |
| MS.gene071381.t1 | AT5G63530 | 58.099 | 284 | 104 | 4 | 52 | 322 | 59 | 340 | 8.14e-77 | 239 |
| MS.gene071381.t1 | AT5G50740 | 49.689 | 322 | 130 | 5 | 1 | 322 | 1 | 290 | 1.28e-76 | 236 |
| MS.gene071381.t1 | AT5G63530 | 58.099 | 284 | 104 | 4 | 52 | 322 | 74 | 355 | 1.72e-76 | 238 |
| MS.gene071381.t1 | AT3G02960 | 38.745 | 271 | 105 | 5 | 52 | 322 | 37 | 246 | 6.14e-50 | 166 |
| MS.gene071381.t1 | AT5G50740 | 61.417 | 127 | 42 | 2 | 53 | 177 | 29 | 150 | 1.64e-45 | 152 |
| MS.gene071381.t1 | AT5G50740 | 50.838 | 179 | 64 | 4 | 1 | 177 | 1 | 157 | 3.51e-39 | 136 |
| MS.gene071381.t1 | AT5G24580 | 33.333 | 282 | 154 | 6 | 53 | 321 | 57 | 317 | 3.66e-32 | 122 |
| MS.gene071381.t1 | AT5G24580 | 33.333 | 282 | 154 | 6 | 53 | 321 | 58 | 318 | 3.81e-32 | 122 |
| MS.gene071381.t1 | AT5G24580 | 33.333 | 282 | 154 | 6 | 53 | 321 | 58 | 318 | 3.81e-32 | 122 |
| MS.gene071381.t1 | AT5G24580 | 32.270 | 282 | 154 | 7 | 53 | 321 | 58 | 315 | 5.47e-29 | 114 |
| MS.gene071381.t1 | AT2G36950 | 31.751 | 337 | 161 | 9 | 53 | 322 | 52 | 386 | 6.17e-29 | 115 |
| MS.gene071381.t1 | AT5G03380 | 35.556 | 270 | 135 | 8 | 26 | 264 | 3 | 264 | 2.67e-28 | 113 |
| MS.gene071381.t1 | AT5G03380 | 35.270 | 241 | 121 | 6 | 55 | 264 | 1 | 237 | 5.98e-26 | 106 |
| MS.gene071381.t1 | AT5G60800 | 35.612 | 278 | 149 | 5 | 53 | 322 | 28 | 283 | 1.64e-22 | 95.5 |
| MS.gene071381.t1 | AT5G60800 | 35.252 | 278 | 150 | 5 | 53 | 322 | 28 | 283 | 2.09e-21 | 92.8 |
| MS.gene071381.t1 | AT5G24580 | 31.474 | 251 | 138 | 6 | 84 | 321 | 2 | 231 | 6.77e-21 | 90.1 |
| MS.gene071381.t1 | AT1G29000 | 33.333 | 165 | 104 | 3 | 54 | 216 | 18 | 178 | 3.72e-19 | 86.3 |
| MS.gene071381.t1 | AT1G29000 | 33.333 | 165 | 104 | 3 | 54 | 216 | 18 | 178 | 3.72e-19 | 86.3 |
| MS.gene071381.t1 | AT2G28090 | 29.907 | 214 | 136 | 3 | 36 | 247 | 14 | 215 | 3.17e-16 | 77.4 |
Find 0 sgRNAs with CRISPR-Local
Find 96 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AGTTAAGATATTTTATAATA+AGG | - | 57542:43068-43087 | MS.gene071381:intergenic | 10.0% |
| !!! | CATTTTAATAATATTGTTTC+TGG | + | 57542:42120-42139 | MS.gene071381:intron | 15.0% |
| !! | CTTGATAAAATATTTGTAAG+TGG | + | 57542:43086-43105 | MS.gene071381:intron | 20.0% |
| !! | TAATGATTAATTGTTTGTGA+TGG | + | 57542:42442-42461 | MS.gene071381:intron | 20.0% |
| !! | TTAAAATTATATTCACATGC+AGG | + | 57542:43155-43174 | MS.gene071381:intron | 20.0% |
| !!! | AATATTATTTTAATCCACCA+AGG | + | 57542:43504-43523 | MS.gene071381:CDS | 20.0% |
| !!! | AGAAAAAAAGTGTAAAGAAA+AGG | - | 57542:42409-42428 | MS.gene071381:intergenic | 20.0% |
| !!! | TTTTTGTTGTTGTTTTGAAT+AGG | + | 57542:42150-42169 | MS.gene071381:intron | 20.0% |
| ! | AAATTTATCTGAATCTGTGA+TGG | + | 57542:42720-42739 | MS.gene071381:intron | 25.0% |
| !! | TTTTCATCACTGAAAATTTG+AGG | - | 57542:43572-43591 | MS.gene071381:intergenic | 25.0% |
| !!! | AGTTTTTTTCTCTTCTTCTT+TGG | - | 57542:43336-43355 | MS.gene071381:intergenic | 25.0% |
| !!! | ATTTTTTTCTCTTGTTCTGA+AGG | - | 57542:42617-42636 | MS.gene071381:intergenic | 25.0% |
| AAATCTGACAAATTCCTGAA+AGG | - | 57542:42962-42981 | MS.gene071381:intergenic | 30.0% | |
| AAGACTTTAAGCACAATTTC+TGG | - | 57542:42316-42335 | MS.gene071381:intergenic | 30.0% | |
| AAGGTGAAAAAGATCAGAAA+GGG | + | 57542:43360-43379 | MS.gene071381:CDS | 30.0% | |
| AGAAGAAGAGAAAAAAACTG+AGG | + | 57542:43337-43356 | MS.gene071381:CDS | 30.0% | |
| AGAATCACTATCATAACCTT+AGG | - | 57542:42782-42801 | MS.gene071381:intergenic | 30.0% | |
| GAATATGTTTACAAGAGAAC+TGG | + | 57542:43254-43273 | MS.gene071381:CDS | 30.0% | |
| TACATGATAGAACAAGCATT+TGG | - | 57542:43596-43615 | MS.gene071381:intergenic | 30.0% | |
| TGTTGTTAAAGGAGAAAAGA+CGG | + | 57542:42514-42533 | MS.gene071381:CDS | 30.0% | |
| TTCTTCTCTTCTTCTTTCTT+CGG | - | 57542:42229-42248 | MS.gene071381:intergenic | 30.0% | |
| TTTATTTCACTCACTTGGTT+TGG | + | 57542:42917-42936 | MS.gene071381:intron | 30.0% | |
| !! | TCACTTTTATTTCACTCACT+TGG | + | 57542:42912-42931 | MS.gene071381:intron | 30.0% |
| !! | TCTTCTTCTTTGGTTTCTTT+TGG | - | 57542:43326-43345 | MS.gene071381:intergenic | 30.0% |
| !!! | TATTTTAATCCACCAAGGTA+TGG | + | 57542:43509-43528 | MS.gene071381:CDS | 30.0% |
| !!! | TGTTTGTGATGGATTTTTCA+GGG | + | 57542:42453-42472 | MS.gene071381:intron | 30.0% |
| !!! | TTGTTTGTGATGGATTTTTC+AGG | + | 57542:42452-42471 | MS.gene071381:intron | 30.0% |
| !!! | TTTGGATGTTCTTTTTTCTC+TGG | - | 57542:43308-43327 | MS.gene071381:intergenic | 30.0% |
| AAAGTCTTCATGCATTGTGA+AGG | + | 57542:42328-42347 | MS.gene071381:CDS | 35.0% | |
| AACTCATTAGTGACAGTGAA+AGG | + | 57542:43206-43225 | MS.gene071381:CDS | 35.0% | |
| ACTAATGAGTTCTTCAGATC+CGG | - | 57542:43197-43216 | MS.gene071381:intergenic | 35.0% | |
| ACTCTTTCAAGAACCTTCAA+TGG | - | 57542:42542-42561 | MS.gene071381:intergenic | 35.0% | |
| AGACGCATTGAGAAAATCAA+AGG | + | 57542:42845-42864 | MS.gene071381:CDS | 35.0% | |
| ATAGGAAGAAAAGAAACCTG+AGG | + | 57542:42168-42187 | MS.gene071381:intron | 35.0% | |
| CATCACTGAAAATTTGAGGA+GGG | - | 57542:43568-43587 | MS.gene071381:intergenic | 35.0% | |
| GAAGAGAAAAAAACTGAGGA+AGG | + | 57542:43341-43360 | MS.gene071381:CDS | 35.0% | |
| GAAGGTGAAAAAGATCAGAA+AGG | + | 57542:43359-43378 | MS.gene071381:CDS | 35.0% | |
| GACAGAAGAAGAGAAAAAAC+CGG | + | 57542:42267-42286 | MS.gene071381:CDS | 35.0% | |
| TCATCACTGAAAATTTGAGG+AGG | - | 57542:43569-43588 | MS.gene071381:intergenic | 35.0% | |
| TGAAGAAAAGAAAGCTGAAG+AGG | + | 57542:42664-42683 | MS.gene071381:CDS | 35.0% | |
| TGACAAATTCCTGAAAGGAT+GGG | - | 57542:42957-42976 | MS.gene071381:intergenic | 35.0% | |
| TTATTATATGGTGCAGCCTA+AGG | + | 57542:42763-42782 | MS.gene071381:intron | 35.0% | |
| ! | ACTTTAAGCACAATTTCTGG+CGG | - | 57542:42313-42332 | MS.gene071381:intergenic | 35.0% |
| ! | TACTGATTGCAAATCTCACA+AGG | + | 57542:42490-42509 | MS.gene071381:CDS | 35.0% |
| ! | TCAGCTTTCTTTTCTTCATG+TGG | - | 57542:42662-42681 | MS.gene071381:intergenic | 35.0% |
| ! | TCTCACAAGGTTGTTGTTAA+AGG | + | 57542:42503-42522 | MS.gene071381:CDS | 35.0% |
| ! | TTCTTTTCTTCATGTGGCTT+AGG | - | 57542:42656-42675 | MS.gene071381:intergenic | 35.0% |
| !!! | GGTTTTTTCTCTTCTTCTGT+CGG | - | 57542:42268-42287 | MS.gene071381:intergenic | 35.0% |
| !!! | GTTTGTGATGGATTTTTCAG+GGG | + | 57542:42454-42473 | MS.gene071381:intron | 35.0% |
| !!! | TTTTTCTCTTGTTCTGAAGG+TGG | - | 57542:42614-42633 | MS.gene071381:intergenic | 35.0% |
| AAAATTTGAGGAGGGTAAGC+TGG | - | 57542:43560-43579 | MS.gene071380:intergenic | 40.0% | |
| AAAGGCAAAAACTCTTCACC+TGG | - | 57542:42391-42410 | MS.gene071380:intergenic | 40.0% | |
| AAAGTTCGTCGTTCACTCAA+AGG | + | 57542:42361-42380 | MS.gene071381:CDS | 40.0% | |
| AGAAAAGACGGATCCATTGA+AGG | + | 57542:42526-42545 | MS.gene071381:CDS | 40.0% | |
| AGAAGAAGAGAAAAAACCGG+AGG | + | 57542:42270-42289 | MS.gene071381:CDS | 40.0% | |
| AGGTTCTTGAAAGAGTTCAG+AGG | + | 57542:42546-42565 | MS.gene071381:CDS | 40.0% | |
| AGTAGAGGAGAAAAAACCAG+AGG | + | 57542:42198-42217 | MS.gene071381:CDS | 40.0% | |
| CTGACAAATTCCTGAAAGGA+TGG | - | 57542:42958-42977 | MS.gene071381:intergenic | 40.0% | |
| GAAAAAACCGGAGGAATCTA+AGG | + | 57542:42279-42298 | MS.gene071381:CDS | 40.0% | |
| GAAAAAGATCAGAAAGGGAG+TGG | + | 57542:43365-43384 | MS.gene071381:CDS | 40.0% | |
| GTAACTTCCTTAGATTCCTC+CGG | - | 57542:42289-42308 | MS.gene071381:intergenic | 40.0% | |
| TAAAACTCCGTACCATACCT+TGG | - | 57542:43524-43543 | MS.gene071381:intergenic | 40.0% | |
| TAATCCACCAAGGTATGGTA+CGG | + | 57542:43514-43533 | MS.gene071381:CDS | 40.0% | |
| TTCTCTTCTTCTTTCTTCGG+TGG | - | 57542:42226-42245 | MS.gene071381:intergenic | 40.0% | |
| ! | TTTTTCTCTTCTTCTGTCGG+TGG | - | 57542:42265-42284 | MS.gene071381:intergenic | 40.0% |
| !! | ACGGAGTTTTATGCATATCC+TGG | + | 57542:43533-43552 | MS.gene071381:CDS | 40.0% |
| !! | CTTGGTTTGGTTCAATTCTC+AGG | + | 57542:42930-42949 | MS.gene071381:intron | 40.0% |
| !!! | CTTGTTCTGAAGGTGGTTTT+GGG | - | 57542:42607-42626 | MS.gene071381:intergenic | 40.0% |
| !!! | TCTTGTTCTGAAGGTGGTTT+TGG | - | 57542:42608-42627 | MS.gene071381:intergenic | 40.0% |
| ACCTGAGGAAACGAAAGTAG+AGG | + | 57542:42183-42202 | MS.gene071381:CDS | 45.0% | |
| AGGAGTTGAATCAGCTGAAC+CGG | + | 57542:43175-43194 | MS.gene071381:intron | 45.0% | |
| AGGTGGTGCAGAAGAAGTTA+AGG | + | 57542:43460-43479 | MS.gene071381:CDS | 45.0% | |
| GATGGAAAACCAAGTGGTGA+TGG | + | 57542:43434-43453 | MS.gene071381:CDS | 45.0% | |
| TCCTCTACTTTCGTTTCCTC+AGG | - | 57542:42187-42206 | MS.gene071381:intergenic | 45.0% | |
| TGCAGAAGAAGTTAAGGTGG+TGG | + | 57542:43466-43485 | MS.gene071381:CDS | 45.0% | |
| TGGTGCAGAAGAAGTTAAGG+TGG | + | 57542:43463-43482 | MS.gene071381:CDS | 45.0% | |
| ! | CGTTCACTCAAAGGTTTTCC+AGG | + | 57542:42370-42389 | MS.gene071381:CDS | 45.0% |
| !!! | TCTGAAGGTGGTTTTGGGAT+CGG | - | 57542:42602-42621 | MS.gene071381:intergenic | 45.0% |
| !!! | ATCATTTTTATTTTATTATA+TGG | + | 57542:42751-42770 | MS.gene071381:intron | 5.0% |
| AACTCCGTACCATACCTTGG+TGG | - | 57542:43521-43540 | MS.gene071381:intergenic | 50.0% | |
| AAGTGGTCCTGCAAGTGCAA+AGG | + | 57542:43103-43122 | MS.gene071381:intron | 50.0% | |
| AGTGGTCCTGCAAGTGCAAA+GGG | + | 57542:43104-43123 | MS.gene071381:intron | 50.0% | |
| GAGGGAGATGGAAAACCAAG+TGG | + | 57542:43428-43447 | MS.gene071381:CDS | 50.0% | |
| GATCAGAAAGGGAGTGGTGA+AGG | + | 57542:43371-43390 | MS.gene071381:CDS | 50.0% | |
| TCTTTCTTCGGTGGTTCCTC+TGG | - | 57542:42217-42236 | MS.gene071381:intergenic | 50.0% | |
| TTCTCAGGTCCCATCCTTTC+AGG | + | 57542:42945-42964 | MS.gene071381:intron | 50.0% | |
| !! | AAACCAAGTGGTGATGGAGG+AGG | + | 57542:43440-43459 | MS.gene071381:CDS | 50.0% |
| !! | GGAAAACCAAGTGGTGATGG+AGG | + | 57542:43437-43456 | MS.gene071381:CDS | 50.0% |
| GAAAGGGAGTGGTGAAGGAG+AGG | + | 57542:43376-43395 | MS.gene071381:CDS | 55.0% | |
| GTCACACCCTTTGCACTTGC+AGG | - | 57542:43113-43132 | MS.gene071381:intergenic | 55.0% | |
| CCACCTCCTCCATCACCACT+TGG | - | 57542:43446-43465 | MS.gene071381:intergenic | 60.0% | |
| TGAGGAGGGTAAGCTGGACC+AGG | - | 57542:43554-43573 | MS.gene071381:intergenic | 60.0% | |
| !! | CCAAGTGGTGATGGAGGAGG+TGG | + | 57542:43443-43462 | MS.gene071381:CDS | 60.0% |
| AGCTGCAGCTGCAGCTGCAG+AGG | + | 57542:43409-43428 | MS.gene071381:CDS | 65.0% | |
| GCTGCAGCTGCAGAGGGAGA+TGG | + | 57542:43416-43435 | MS.gene071381:CDS | 65.0% | |
| GCTGCAGCTGCAGCTGCAGA+GGG | + | 57542:43410-43429 | MS.gene071381:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 57542 | gene | 42051 | 43616 | 42051 | ID=MS.gene071381 |
| 57542 | mRNA | 42051 | 43616 | 42051 | ID=MS.gene071381.t1;Parent=MS.gene071381 |
| 57542 | exon | 42051 | 42059 | 42051 | ID=MS.gene071381.t1.exon1;Parent=MS.gene071381.t1 |
| 57542 | CDS | 42051 | 42059 | 42051 | ID=cds.MS.gene071381.t1;Parent=MS.gene071381.t1 |
| 57542 | exon | 42172 | 42391 | 42172 | ID=MS.gene071381.t1.exon2;Parent=MS.gene071381.t1 |
| 57542 | CDS | 42172 | 42391 | 42172 | ID=cds.MS.gene071381.t1;Parent=MS.gene071381.t1 |
| 57542 | exon | 42474 | 42685 | 42474 | ID=MS.gene071381.t1.exon3;Parent=MS.gene071381.t1 |
| 57542 | CDS | 42474 | 42685 | 42474 | ID=cds.MS.gene071381.t1;Parent=MS.gene071381.t1 |
| 57542 | exon | 42779 | 42866 | 42779 | ID=MS.gene071381.t1.exon4;Parent=MS.gene071381.t1 |
| 57542 | CDS | 42779 | 42866 | 42779 | ID=cds.MS.gene071381.t1;Parent=MS.gene071381.t1 |
| 57542 | exon | 43177 | 43616 | 43177 | ID=MS.gene071381.t1.exon5;Parent=MS.gene071381.t1 |
| 57542 | CDS | 43177 | 43616 | 43177 | ID=cds.MS.gene071381.t1;Parent=MS.gene071381.t1 |
| Gene Sequence |
| Protein sequence |
