Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene071508.t1 | XP_024637044.1 | 96.6 | 58 | 2 | 0 | 36 | 93 | 243 | 300 | 5.20E-24 | 120.2 |
| MS.gene071508.t1 | XP_024637044.1 | 69.4 | 36 | 11 | 0 | 1 | 36 | 1 | 36 | 7.10E-05 | 56.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene071508.t1 | O49726 | 68.5 | 54 | 17 | 0 | 38 | 91 | 247 | 300 | 2.9e-15 | 82.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene071508.t1 | A0A396IJN7 | 96.6 | 58 | 2 | 0 | 36 | 93 | 243 | 300 | 3.8e-24 | 120.2 |
| MS.gene071508.t1 | A0A396IJN7 | 69.4 | 36 | 11 | 0 | 1 | 36 | 1 | 36 | 5.1e-05 | 56.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene058769 | MS.gene071508 | 0.812017 | 5.24E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene071508.t1 | MTR_4g098900 | 93.103 | 58 | 4 | 0 | 36 | 93 | 243 | 300 | 8.22e-33 | 117 |
| MS.gene071508.t1 | MTR_4g098900 | 88.889 | 36 | 4 | 0 | 1 | 36 | 1 | 36 | 9.84e-12 | 59.7 |
| MS.gene071508.t1 | MTR_2g015470 | 68.966 | 58 | 18 | 0 | 36 | 93 | 245 | 302 | 4.74e-22 | 88.2 |
| MS.gene071508.t1 | MTR_6g082540 | 57.407 | 54 | 23 | 0 | 38 | 91 | 40 | 93 | 1.58e-18 | 75.5 |
| MS.gene071508.t1 | MTR_7g080750 | 56.897 | 58 | 25 | 0 | 36 | 93 | 216 | 273 | 3.22e-18 | 77.4 |
| MS.gene071508.t1 | MTR_3g058660 | 61.111 | 54 | 21 | 0 | 38 | 91 | 222 | 275 | 1.75e-17 | 75.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene071508.t1 | AT4G18220 | 67.273 | 55 | 18 | 0 | 37 | 91 | 246 | 300 | 2.79e-19 | 80.9 |
| MS.gene071508.t1 | AT1G44750 | 56.897 | 58 | 25 | 0 | 36 | 93 | 167 | 224 | 6.66e-19 | 79.0 |
| MS.gene071508.t1 | AT1G44750 | 56.897 | 58 | 25 | 0 | 36 | 93 | 167 | 224 | 6.66e-19 | 79.0 |
| MS.gene071508.t1 | AT1G44750 | 56.897 | 58 | 25 | 0 | 36 | 93 | 167 | 224 | 6.66e-19 | 79.0 |
| MS.gene071508.t1 | AT1G44750 | 56.897 | 58 | 25 | 0 | 36 | 93 | 167 | 224 | 6.66e-19 | 79.0 |
| MS.gene071508.t1 | AT1G44750 | 56.897 | 58 | 25 | 0 | 36 | 93 | 167 | 224 | 6.66e-19 | 79.0 |
| MS.gene071508.t1 | AT4G18210 | 67.273 | 55 | 18 | 0 | 37 | 91 | 246 | 300 | 1.83e-18 | 78.6 |
| MS.gene071508.t1 | AT1G44750 | 56.897 | 58 | 25 | 0 | 36 | 93 | 243 | 300 | 5.57e-18 | 77.0 |
| MS.gene071508.t1 | AT1G44750 | 56.897 | 58 | 25 | 0 | 36 | 93 | 231 | 288 | 5.71e-18 | 77.0 |
| MS.gene071508.t1 | AT1G44750 | 56.897 | 58 | 25 | 0 | 36 | 93 | 231 | 288 | 5.71e-18 | 77.0 |
| MS.gene071508.t1 | AT4G18195 | 58.929 | 56 | 23 | 0 | 38 | 93 | 250 | 305 | 5.90e-18 | 77.0 |
| MS.gene071508.t1 | AT4G18197 | 53.571 | 56 | 26 | 0 | 38 | 93 | 247 | 302 | 5.84e-17 | 74.3 |
| MS.gene071508.t1 | AT4G18205 | 59.259 | 54 | 22 | 0 | 38 | 91 | 244 | 297 | 1.29e-16 | 73.2 |
| MS.gene071508.t1 | AT4G08700 | 51.786 | 56 | 27 | 0 | 36 | 91 | 232 | 287 | 4.38e-14 | 66.2 |
| MS.gene071508.t1 | AT4G18190 | 55.556 | 54 | 24 | 0 | 38 | 91 | 244 | 297 | 4.61e-14 | 66.2 |
| MS.gene071508.t1 | AT5G41160 | 48.214 | 56 | 29 | 0 | 36 | 91 | 235 | 290 | 3.86e-12 | 60.8 |
Find 23 sgRNAs with CRISPR-Local
Find 84 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTATCTTCCATTGGTATGTT+AGG | 0.270589 | 8.1:-23672003 | MS.gene071508:CDS |
| CATATATAATGACTTTGTTT+TGG | 0.282504 | 8.1:-23672044 | MS.gene071508:CDS |
| GCTACTTGTGCTTGTGTTGT+TGG | 0.342888 | 8.1:-23672135 | MS.gene071508:CDS |
| TTCTCTAATGGAGATAGTTC+AGG | 0.361251 | 8.1:-23673350 | None:intergenic |
| GGAGATGAAGGAATATGAAC+TGG | 0.366582 | 8.1:-23672076 | MS.gene071508:CDS |
| CTACTTGTGCTTGTGTTGTT+GGG | 0.389251 | 8.1:-23672134 | MS.gene071508:CDS |
| AGAATGGAAAAGTTTGAATA+AGG | 0.399319 | 8.1:-23672097 | MS.gene071508:CDS |
| GCACAAGTAGCGACGAAAGA+AGG | 0.407661 | 8.1:+23672147 | None:intergenic |
| TATCTTCCATTGGTATGTTA+GGG | 0.435640 | 8.1:-23672002 | None:intergenic |
| GGTTGTTTGCAAGTGGAGAA+TGG | 0.463564 | 8.1:-23672113 | MS.gene071508:CDS |
| AAAGATTAAGAGATTTCAAA+TGG | 0.470365 | 8.1:-23672803 | MS.gene071508:CDS |
| TCTCTTAATCTTTGATGTTG+AGG | 0.470898 | 8.1:+23672813 | None:intergenic |
| CAGTAGTTGTAGTTGATTGT+GGG | 0.484203 | 8.1:+23673320 | None:intergenic |
| AAGTTTGAATAAGGAGATGA+AGG | 0.509849 | 8.1:-23672088 | MS.gene071508:CDS |
| GCAGTAGTTGTAGTTGATTG+TGG | 0.527047 | 8.1:+23673319 | None:intergenic |
| CACAAGTAGCGACGAAAGAA+GGG | 0.536717 | 8.1:+23672148 | None:intergenic |
| GTTGTTGGGTTGTTTGCAAG+TGG | 0.551141 | 8.1:-23672120 | MS.gene071508:CDS |
| CAATCAACTACAACTACTGC+AGG | 0.567854 | 8.1:-23673316 | MS.gene071508:intron |
| AACTAACCCTAACATACCAA+TGG | 0.573549 | 8.1:+23671996 | None:intergenic |
| ACATGGCAAGTATCTTCCAT+TGG | 0.573996 | 8.1:-23672012 | MS.gene071508:CDS |
| GATTAAGAGATTTCAAATGG+TGG | 0.581181 | 8.1:-23672800 | MS.gene071508:CDS |
| GAAGGAATATGAACTGGGAA+AGG | 0.585101 | 8.1:-23672070 | MS.gene071508:CDS |
| GAGATGAAGGAATATGAACT+GGG | 0.604093 | 8.1:-23672075 | MS.gene071508:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATCAATATTGATATTTATGT+TGG | + | chr8.1:23672750-23672769 | None:intergenic | 15.0% |
| !! | AAAGATTAAGAGATTTCAAA+TGG | - | chr8.1:23672543-23672562 | MS.gene071508:intron | 20.0% |
| !! | AGAAATTCATAACAAGAAAT+TGG | + | chr8.1:23672493-23672512 | None:intergenic | 20.0% |
| !! | GAAATTCATAACAAGAAATT+GGG | + | chr8.1:23672492-23672511 | None:intergenic | 20.0% |
| !! | TCCTAACTATTGAAAATTAT+TGG | - | chr8.1:23672281-23672300 | MS.gene071508:intron | 20.0% |
| !!! | AAATTTTGGTTTGGTTTTTT+TGG | + | chr8.1:23672777-23672796 | None:intergenic | 20.0% |
| !!! | ATAATTTTCAATAGTTAGGA+AGG | + | chr8.1:23672281-23672300 | None:intergenic | 20.0% |
| !!! | CATATATAATGACTTTGTTT+TGG | - | chr8.1:23673302-23673321 | MS.gene071508:intron | 20.0% |
| !!! | GTTTCTCTTTACATATTTTT+TGG | - | chr8.1:23672806-23672825 | MS.gene071508:CDS | 20.0% |
| ! | AGAATGGAAAAGTTTGAATA+AGG | - | chr8.1:23673249-23673268 | MS.gene071508:intron | 25.0% |
| ! | AGACTATATTATGATAAAGG+TGG | - | chr8.1:23672632-23672651 | MS.gene071508:intron | 25.0% |
| ! | ATAAAGGTGGTAATAGTAAA+TGG | - | chr8.1:23672645-23672664 | MS.gene071508:intron | 25.0% |
| ! | GGAAGACTATATTATGATAA+AGG | - | chr8.1:23672629-23672648 | MS.gene071508:intron | 25.0% |
| ! | TATGTAAAGAGAAACAATGA+TGG | + | chr8.1:23672801-23672820 | None:intergenic | 25.0% |
| !! | AATACATCAAAACTTGCTTT+AGG | - | chr8.1:23672255-23672274 | MS.gene071508:intron | 25.0% |
| !! | GATATTTATGTTGGTAGATT+TGG | + | chr8.1:23672741-23672760 | None:intergenic | 25.0% |
| !! | GCCAATAATTTTCAATAGTT+AGG | + | chr8.1:23672285-23672304 | None:intergenic | 25.0% |
| !! | TACATAGTATTTCTTTTGGT+AGG | - | chr8.1:23672584-23672603 | MS.gene071508:intron | 25.0% |
| !! | TCTCTACATAGTATTTCTTT+TGG | - | chr8.1:23672580-23672599 | MS.gene071508:intron | 25.0% |
| !!! | AATGATGGAAAATTTTGGTT+TGG | + | chr8.1:23672786-23672805 | None:intergenic | 25.0% |
| !!! | GAAACAATGATGGAAAATTT+TGG | + | chr8.1:23672791-23672810 | None:intergenic | 25.0% |
| AAAATGTAATAGATGCACCA+AGG | + | chr8.1:23673093-23673112 | None:intergenic | 30.0% | |
| AAATAGAGTGGAAGTAGTAT+AGG | + | chr8.1:23672701-23672720 | None:intergenic | 30.0% | |
| AAATGTAATAGATGCACCAA+GGG | + | chr8.1:23673092-23673111 | None:intergenic | 30.0% | |
| AAGTTTGAATAAGGAGATGA+AGG | - | chr8.1:23673258-23673277 | MS.gene071508:intron | 30.0% | |
| AATAGAGTGGAAGTAGTATA+GGG | + | chr8.1:23672700-23672719 | None:intergenic | 30.0% | |
| ATATAGTCTTCCCAAAAGAT+TGG | + | chr8.1:23672621-23672640 | None:intergenic | 30.0% | |
| ATCATCAGAATCGTTATTGA+TGG | + | chr8.1:23673023-23673042 | None:intergenic | 30.0% | |
| GACAATTTGATGTATTCCAA+TGG | - | chr8.1:23672845-23672864 | MS.gene071508:intron | 30.0% | |
| GATTAAGAGATTTCAAATGG+TGG | - | chr8.1:23672546-23672565 | MS.gene071508:intron | 30.0% | |
| GTAAGAAAATACTGCATTGA+AGG | + | chr8.1:23672924-23672943 | None:intergenic | 30.0% | |
| TCAGTAGTGAAAGATTAACA+TGG | + | chr8.1:23672307-23672326 | None:intergenic | 30.0% | |
| TCTCTTAATCTTTGATGTTG+AGG | + | chr8.1:23672536-23672555 | None:intergenic | 30.0% | |
| TGAAAGATATAGAAGTCCAT+TGG | + | chr8.1:23672864-23672883 | None:intergenic | 30.0% | |
| TGTTGCACATAGTAAAGAAT+AGG | + | chr8.1:23672894-23672913 | None:intergenic | 30.0% | |
| ! | ACACAAAGTTGAGTCAATTT+TGG | + | chr8.1:23672372-23672391 | None:intergenic | 30.0% |
| ! | ATATGAATGCTGTGAACTTT+TGG | + | chr8.1:23672958-23672977 | None:intergenic | 30.0% |
| ! | TATGAATGCTGTGAACTTTT+GGG | + | chr8.1:23672957-23672976 | None:intergenic | 30.0% |
| ! | TCTTGTTATGAATTTCTCAG+AGG | - | chr8.1:23672496-23672515 | MS.gene071508:intron | 30.0% |
| !!! | TTTTGGTTTGGTTTTTTTGG+AGG | + | chr8.1:23672774-23672793 | None:intergenic | 30.0% |
| !!! | TTTTTGGCTTACTTTTAGCA+GGG | - | chr8.1:23672822-23672841 | MS.gene071508:CDS | 30.0% |
| !!! | TTTTTTGGCTTACTTTTAGC+AGG | - | chr8.1:23672821-23672840 | MS.gene071508:CDS | 30.0% |
| AAACTGCATGTCCAAGATAA+CGG | + | chr8.1:23673176-23673195 | None:intergenic | 35.0% | |
| AGGTGGTAATAGTAAATGGA+TGG | - | chr8.1:23672649-23672668 | MS.gene071508:intron | 35.0% | |
| CAGTAGTTGTAGTTGATTGT+GGG | + | chr8.1:23672029-23672048 | None:intergenic | 35.0% | |
| GAGATGAAGGAATATGAACT+GGG | - | chr8.1:23673271-23673290 | MS.gene071508:intron | 35.0% | |
| GGTGTGAAGATAAAATAGAG+TGG | + | chr8.1:23672713-23672732 | None:intergenic | 35.0% | |
| GTATCTTCCATTGGTATGTT+AGG | - | chr8.1:23673343-23673362 | MS.gene071508:CDS | 35.0% | |
| TAGAACAATGAACACCAGTA+CGG | + | chr8.1:23672436-23672455 | None:intergenic | 35.0% | |
| TCTGATAAATCACATGTCGA+TGG | - | chr8.1:23672228-23672247 | MS.gene071508:intron | 35.0% | |
| TGCACATAGTAAAGAATAGG+TGG | + | chr8.1:23672891-23672910 | None:intergenic | 35.0% | |
| TTTGACTATCAGACTTTGAG+AGG | + | chr8.1:23672198-23672217 | None:intergenic | 35.0% | |
| ! | AATCGTTATTGATGGCAAGT+AGG | + | chr8.1:23673015-23673034 | None:intergenic | 35.0% |
| ! | ATCGTTATTGATGGCAAGTA+GGG | + | chr8.1:23673014-23673033 | None:intergenic | 35.0% |
| ! | CAATCTACAGCCAATCTTTT+GGG | - | chr8.1:23672608-23672627 | MS.gene071508:intron | 35.0% |
| ! | TCAATCTACAGCCAATCTTT+TGG | - | chr8.1:23672607-23672626 | MS.gene071508:intron | 35.0% |
| ! | TGAAACCTTTTCCGTTATCT+TGG | - | chr8.1:23673162-23673181 | MS.gene071508:intron | 35.0% |
| !! | CTGTGAACTTTTGGGAATTA+AGG | + | chr8.1:23672949-23672968 | None:intergenic | 35.0% |
| !!! | TTTTGGCTTACTTTTAGCAG+GGG | - | chr8.1:23672823-23672842 | MS.gene071508:CDS | 35.0% |
| AACTACAACTACTGCAGGTT+AGG | - | chr8.1:23672035-23672054 | MS.gene071508:CDS | 40.0% | |
| ACATGGCAAGTATCTTCCAT+TGG | - | chr8.1:23673334-23673353 | MS.gene071508:CDS | 40.0% | |
| CAATCAACTACAACTACTGC+AGG | - | chr8.1:23672030-23672049 | MS.gene071508:CDS | 40.0% | |
| CCAAGTACTCGCTTATCTTT+AGG | + | chr8.1:23673058-23673077 | None:intergenic | 40.0% | |
| CCTAAAGATAAGCGAGTACT+TGG | - | chr8.1:23673055-23673074 | MS.gene071508:intron | 40.0% | |
| GAAGGAATATGAACTGGGAA+AGG | - | chr8.1:23673276-23673295 | MS.gene071508:intron | 40.0% | |
| GCAACATTTGTTCAATCAGC+TGG | - | chr8.1:23672671-23672690 | MS.gene071508:intron | 40.0% | |
| GCAGTAGTTGTAGTTGATTG+TGG | + | chr8.1:23672030-23672049 | None:intergenic | 40.0% | |
| GGAGATGAAGGAATATGAAC+TGG | - | chr8.1:23673270-23673289 | MS.gene071508:intron | 40.0% | |
| GTCAAAAGAAAGCTGCACAA+GGG | + | chr8.1:23673128-23673147 | None:intergenic | 40.0% | |
| TGTCAAAAGAAAGCTGCACA+AGG | + | chr8.1:23673129-23673148 | None:intergenic | 40.0% | |
| ! | ATGTTGGTAGATTTGGCATG+TGG | + | chr8.1:23672734-23672753 | None:intergenic | 40.0% |
| ! | CTACTTGTGCTTGTGTTGTT+GGG | - | chr8.1:23673212-23673231 | MS.gene071508:intron | 40.0% |
| !! | CATGTCCAAGATAACGGAAA+AGG | + | chr8.1:23673170-23673189 | None:intergenic | 40.0% |
| !! | TGTTTTGGATTGCTGTGACA+TGG | - | chr8.1:23673317-23673336 | MS.gene071508:CDS | 40.0% |
| ACTACTGCAGGTTAGGAACA+TGG | - | chr8.1:23672042-23672061 | MS.gene071508:CDS | 45.0% | |
| CACAAGTAGCGACGAAAGAA+GGG | + | chr8.1:23673201-23673220 | None:intergenic | 45.0% | |
| GCTACTTGTGCTTGTGTTGT+TGG | - | chr8.1:23673211-23673230 | MS.gene071508:intron | 45.0% | |
| GGTTGTTTGCAAGTGGAGAA+TGG | - | chr8.1:23673233-23673252 | MS.gene071508:intron | 45.0% | |
| ! | GTTGTTGGGTTGTTTGCAAG+TGG | - | chr8.1:23673226-23673245 | MS.gene071508:intron | 45.0% |
| ! | TTTGCTCTTTTGAGCCGTAC+TGG | - | chr8.1:23672419-23672438 | MS.gene071508:intron | 45.0% |
| AAGACACTACCGTTGCTCTG+CGG | - | chr8.1:23672334-23672353 | MS.gene071508:intron | 50.0% | |
| CTTGGATTCGTCTTCACCCT+TGG | - | chr8.1:23673073-23673092 | MS.gene071508:intron | 50.0% | |
| GCACAAGTAGCGACGAAAGA+AGG | + | chr8.1:23673202-23673221 | None:intergenic | 50.0% | |
| TGTCTCTGACCGCAGAGCAA+CGG | + | chr8.1:23672346-23672365 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.1 | gene | 23672003 | 23673365 | 23672003 | ID=MS.gene071508 |
| chr8.1 | mRNA | 23672003 | 23673365 | 23672003 | ID=MS.gene071508.t1;Parent=MS.gene071508 |
| chr8.1 | exon | 23673317 | 23673365 | 23673317 | ID=MS.gene071508.t1.exon1;Parent=MS.gene071508.t1 |
| chr8.1 | CDS | 23673317 | 23673365 | 23673317 | ID=cds.MS.gene071508.t1;Parent=MS.gene071508.t1 |
| chr8.1 | exon | 23672794 | 23672852 | 23672794 | ID=MS.gene071508.t1.exon2;Parent=MS.gene071508.t1 |
| chr8.1 | CDS | 23672794 | 23672852 | 23672794 | ID=cds.MS.gene071508.t1;Parent=MS.gene071508.t1 |
| chr8.1 | exon | 23672003 | 23672175 | 23672003 | ID=MS.gene071508.t1.exon3;Parent=MS.gene071508.t1 |
| chr8.1 | CDS | 23672003 | 23672175 | 23672003 | ID=cds.MS.gene071508.t1;Parent=MS.gene071508.t1 |
| Gene Sequence |
| Protein sequence |
