Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene058769.t1 | XP_003598034.1 | 95.3 | 150 | 7 | 0 | 1 | 150 | 1 | 150 | 2.30E-74 | 288.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene058769.t1 | P56331 | 80.8 | 151 | 22 | 3 | 1 | 150 | 1 | 145 | 2.4e-59 | 229.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene058769.t1 | G7IYF3 | 95.3 | 150 | 7 | 0 | 1 | 150 | 1 | 150 | 1.7e-74 | 288.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene057208 | MS.gene058769 | 0.83284 | 7.51E-56 | -1.69E-46 |
| MS.gene058769 | MS.gene06643 | 0.83564 | 1.49E-56 | -1.69E-46 |
| MS.gene058769 | MS.gene06857 | 0.826246 | 3.01E-54 | -1.69E-46 |
| MS.gene058769 | MS.gene071508 | 0.812017 | 5.24E-51 | -1.69E-46 |
| MS.gene058769 | MS.gene072153 | 0.8373 | 5.64E-57 | -1.69E-46 |
| MS.gene058769 | MS.gene29944 | 0.887772 | 1.06E-72 | -1.69E-46 |
| MS.gene058769 | MS.gene36571 | 0.834104 | 3.63E-56 | -1.69E-46 |
| MS.gene058769 | MS.gene37612 | 0.808536 | 2.95E-50 | -1.69E-46 |
| MS.gene058769 | MS.gene40478 | 0.801784 | 7.65E-49 | -1.69E-46 |
| MS.gene058769 | MS.gene46061 | 0.80409 | 2.55E-49 | -1.69E-46 |
| MS.gene058769 | MS.gene53234 | 0.863682 | 2.09E-64 | -1.69E-46 |
| MS.gene058769 | MS.gene56441 | 0.801999 | 6.91E-49 | -1.69E-46 |
| MS.gene058769 | MS.gene61232 | 0.809715 | 1.65E-50 | -1.69E-46 |
| MS.gene058769 | MS.gene89262 | 0.806811 | 6.86E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene058769.t1 | MTR_3g005520 | 95.333 | 150 | 7 | 0 | 1 | 150 | 1 | 150 | 5.66e-90 | 258 |
| MS.gene058769.t1 | MTR_8g028180 | 84.000 | 150 | 20 | 1 | 1 | 150 | 1 | 146 | 1.75e-74 | 219 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene058769.t1 | AT2G04520 | 82.000 | 150 | 22 | 2 | 1 | 150 | 1 | 145 | 3.55e-77 | 226 |
| MS.gene058769.t1 | AT5G35680 | 80.000 | 150 | 25 | 2 | 1 | 150 | 1 | 145 | 2.75e-71 | 211 |
| MS.gene058769.t1 | AT5G35680 | 80.000 | 150 | 25 | 2 | 1 | 150 | 1 | 145 | 2.75e-71 | 211 |
| MS.gene058769.t1 | AT5G35680 | 86.179 | 123 | 13 | 1 | 1 | 123 | 1 | 119 | 1.58e-69 | 207 |
Find 35 sgRNAs with CRISPR-Local
Find 36 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGTTGGATTACGTGATTATC+AGG | 0.304283 | 3.1:+757334 | MS.gene058769:CDS |
| ACGAGCTTCCGGAATCTATT+AGG | 0.305997 | 3.1:+757420 | MS.gene058769:CDS |
| AACGGAGATATCATTCTTGT+TGG | 0.394753 | 3.1:+757317 | MS.gene058769:CDS |
| GAAGGCTTATGACGAGCTTC+CGG | 0.397993 | 3.1:+757409 | MS.gene058769:CDS |
| TTGAGGATGAGGATATTGAT+AGG | 0.400368 | 3.1:+757513 | MS.gene058769:CDS |
| GTAAGATGCATAAGAAGGTT+TGG | 0.408295 | 3.1:+757288 | MS.gene058769:CDS |
| AGGCTTAATGAAGGGATTGC+TGG | 0.437830 | 3.1:+757440 | MS.gene058769:CDS |
| GCGTCCGTTTCCGAGCATAT+TGG | 0.444772 | 3.1:-757204 | None:intergenic |
| TCATTAAGCCTAATAGATTC+CGG | 0.444954 | 3.1:-757428 | None:intergenic |
| TTCAACAATCTCGCCTCATC+CGG | 0.480596 | 3.1:-757389 | None:intergenic |
| ATGCCGAAGAACAAGGGAAA+AGG | 0.492369 | 3.1:+757089 | MS.gene058769:CDS |
| GCTCGGAAACGGACGCTTGG+AGG | 0.497540 | 3.1:+757211 | MS.gene058769:CDS |
| TGAAGGGATTGCTGGTGGGA+TGG | 0.507121 | 3.1:+757448 | MS.gene058769:CDS |
| ACGTGATTATCAGGATGATA+AGG | 0.510200 | 3.1:+757343 | MS.gene058769:CDS |
| TATGCTCGGAAACGGACGCT+TGG | 0.513890 | 3.1:+757208 | MS.gene058769:CDS |
| GGATGAGGCGAGATTGTTGA+AGG | 0.519335 | 3.1:+757391 | MS.gene058769:CDS |
| GAATCTATTAGGCTTAATGA+AGG | 0.530788 | 3.1:+757431 | MS.gene058769:CDS |
| ATCAACCATGCCGAAGAACA+AGG | 0.554015 | 3.1:+757082 | None:intergenic |
| ATTGAAATATATGCCGGATG+AGG | 0.555641 | 3.1:+757376 | MS.gene058769:CDS |
| GTAACCAATATGCTCGGAAA+CGG | 0.559215 | 3.1:+757200 | MS.gene058769:CDS |
| CCGCGGTAAGATGCATAAGA+AGG | 0.576217 | 3.1:+757283 | MS.gene058769:CDS |
| AAGAAGGTTTGGATTGCTAA+CGG | 0.592201 | 3.1:+757299 | MS.gene058769:CDS |
| CCGAAGAACAAGGGAAAAGG+AGG | 0.596700 | 3.1:+757092 | MS.gene058769:CDS |
| CTTAATGAAGGGATTGCTGG+TGG | 0.602290 | 3.1:+757443 | MS.gene058769:CDS |
| AATCTATTAGGCTTAATGAA+GGG | 0.608999 | 3.1:+757432 | MS.gene058769:CDS |
| ATGGATGATCATGATGATGA+TGG | 0.612732 | 3.1:+757467 | MS.gene058769:CDS |
| TGTCATATTGAAATATATGC+CGG | 0.619355 | 3.1:+757370 | MS.gene058769:CDS |
| TCAACCATGCCGAAGAACAA+GGG | 0.623500 | 3.1:+757083 | None:intergenic |
| GGAGGAAAGAATCGAAAGCG+AGG | 0.627611 | 3.1:+757110 | MS.gene058769:CDS |
| CCTTCTTATGCATCTTACCG+CGG | 0.648150 | 3.1:-757283 | None:intergenic |
| GAACTCATCTTCAAAGAAGA+CGG | 0.648957 | 3.1:+757161 | MS.gene058769:CDS |
| GCACAGGTAACCAATATGCT+CGG | 0.649194 | 3.1:+757194 | MS.gene058769:CDS |
| TTAATGAAGGGATTGCTGGT+GGG | 0.657015 | 3.1:+757444 | MS.gene058769:CDS |
| AGACGGTCAAGAATACGCAC+AGG | 0.669279 | 3.1:+757178 | MS.gene058769:CDS |
| AAACGTCTCTGTCATATCCG+CGG | 0.693679 | 3.1:+757266 | MS.gene058769:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TAATGATTATGTTCATTTTG+AGG | + | chr3.1:757496-757515 | MS.gene058769:CDS | 20.0% |
| ! | AATCTATTAGGCTTAATGAA+GGG | + | chr3.1:757432-757451 | MS.gene058769:CDS | 25.0% |
| ! | TGTCATATTGAAATATATGC+CGG | + | chr3.1:757370-757389 | MS.gene058769:CDS | 25.0% |
| GAATCTATTAGGCTTAATGA+AGG | + | chr3.1:757431-757450 | MS.gene058769:CDS | 30.0% | |
| TCATTAAGCCTAATAGATTC+CGG | - | chr3.1:757431-757450 | None:intergenic | 30.0% | |
| !! | TTATGTTCATTTTGAGGATG+AGG | + | chr3.1:757502-757521 | MS.gene058769:CDS | 30.0% |
| AACGGAGATATCATTCTTGT+TGG | + | chr3.1:757317-757336 | MS.gene058769:CDS | 35.0% | |
| AAGAAGGTTTGGATTGCTAA+CGG | + | chr3.1:757299-757318 | MS.gene058769:CDS | 35.0% | |
| ACGTGATTATCAGGATGATA+AGG | + | chr3.1:757343-757362 | MS.gene058769:CDS | 35.0% | |
| ATTGAAATATATGCCGGATG+AGG | + | chr3.1:757376-757395 | MS.gene058769:CDS | 35.0% | |
| GAACTCATCTTCAAAGAAGA+CGG | + | chr3.1:757161-757180 | MS.gene058769:CDS | 35.0% | |
| GTAAGATGCATAAGAAGGTT+TGG | + | chr3.1:757288-757307 | MS.gene058769:CDS | 35.0% | |
| TGTTGGATTACGTGATTATC+AGG | + | chr3.1:757334-757353 | MS.gene058769:CDS | 35.0% | |
| TTGAGGATGAGGATATTGAT+AGG | + | chr3.1:757513-757532 | MS.gene058769:CDS | 35.0% | |
| ! | ATGGATGATCATGATGATGA+TGG | + | chr3.1:757467-757486 | MS.gene058769:CDS | 35.0% |
| GTAACCAATATGCTCGGAAA+CGG | + | chr3.1:757200-757219 | MS.gene058769:CDS | 40.0% | |
| TTAATGAAGGGATTGCTGGT+GGG | + | chr3.1:757444-757463 | MS.gene058769:CDS | 40.0% | |
| AAACGTCTCTGTCATATCCG+CGG | + | chr3.1:757266-757285 | MS.gene058769:CDS | 45.0% | |
| ACGAGCTTCCGGAATCTATT+AGG | + | chr3.1:757420-757439 | MS.gene058769:CDS | 45.0% | |
| AGGCTTAATGAAGGGATTGC+TGG | + | chr3.1:757440-757459 | MS.gene058769:CDS | 45.0% | |
| ATGCCGAAGAACAAGGGAAA+AGG | + | chr3.1:757089-757108 | MS.gene058769:CDS | 45.0% | |
| CCTTCTTATGCATCTTACCG+CGG | - | chr3.1:757286-757305 | None:intergenic | 45.0% | |
| CTTAATGAAGGGATTGCTGG+TGG | + | chr3.1:757443-757462 | MS.gene058769:CDS | 45.0% | |
| GCACAGGTAACCAATATGCT+CGG | + | chr3.1:757194-757213 | MS.gene058769:CDS | 45.0% | |
| TTCAACAATCTCGCCTCATC+CGG | - | chr3.1:757392-757411 | None:intergenic | 45.0% | |
| ! | CCTCCTTTTCCCTTGTTCTT+CGG | - | chr3.1:757095-757114 | None:intergenic | 45.0% |
| AGACGGTCAAGAATACGCAC+AGG | + | chr3.1:757178-757197 | MS.gene058769:CDS | 50.0% | |
| CCGAAGAACAAGGGAAAAGG+AGG | + | chr3.1:757092-757111 | MS.gene058769:CDS | 50.0% | |
| CCGCGGTAAGATGCATAAGA+AGG | + | chr3.1:757283-757302 | MS.gene058769:CDS | 50.0% | |
| GAAGGCTTATGACGAGCTTC+CGG | + | chr3.1:757409-757428 | MS.gene058769:CDS | 50.0% | |
| GGAGGAAAGAATCGAAAGCG+AGG | + | chr3.1:757110-757129 | MS.gene058769:CDS | 50.0% | |
| GGATGAGGCGAGATTGTTGA+AGG | + | chr3.1:757391-757410 | MS.gene058769:CDS | 50.0% | |
| GCGTCCGTTTCCGAGCATAT+TGG | - | chr3.1:757207-757226 | None:intergenic | 55.0% | |
| TATGCTCGGAAACGGACGCT+TGG | + | chr3.1:757208-757227 | MS.gene058769:CDS | 55.0% | |
| TGAAGGGATTGCTGGTGGGA+TGG | + | chr3.1:757448-757467 | MS.gene058769:CDS | 55.0% | |
| GCTCGGAAACGGACGCTTGG+AGG | + | chr3.1:757211-757230 | MS.gene058769:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.1 | gene | 757089 | 757541 | 757089 | ID=MS.gene058769 |
| chr3.1 | mRNA | 757089 | 757541 | 757089 | ID=MS.gene058769.t1;Parent=MS.gene058769 |
| chr3.1 | exon | 757089 | 757541 | 757089 | ID=MS.gene058769.t1.exon1;Parent=MS.gene058769.t1 |
| chr3.1 | CDS | 757089 | 757541 | 757089 | ID=cds.MS.gene058769.t1;Parent=MS.gene058769.t1 |
| Gene Sequence |
| Protein sequence |
