Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene071648.t1 | RWB92677.1 | 99.5 | 212 | 1 | 0 | 1 | 212 | 6 | 217 | 3.70E-110 | 407.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene071648.t1 | O05000 | 90.9 | 198 | 18 | 0 | 12 | 209 | 241 | 438 | 3.0e-92 | 339.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene071648.t1 | A0A3S3KEP2 | 99.5 | 212 | 1 | 0 | 1 | 212 | 6 | 217 | 2.7e-110 | 407.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene055841 | MS.gene071648 | -0.803247 | 3.82E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene071648.t1 | MTR_0002s0310 | 33.784 | 222 | 117 | 4 | 18 | 209 | 127 | 348 | 9.07e-16 | 75.5 |
| MS.gene071648.t1 | MTR_3g035660 | 33.981 | 206 | 132 | 4 | 8 | 209 | 601 | 806 | 1.20e-14 | 72.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene071648.t1 | AT2G07689 | 96.392 | 194 | 7 | 0 | 19 | 212 | 1 | 194 | 9.79e-131 | 367 |
| MS.gene071648.t1 | ATCG01250 | 37.129 | 202 | 123 | 3 | 12 | 209 | 121 | 322 | 4.78e-19 | 84.7 |
| MS.gene071648.t1 | ATCG00890 | 37.129 | 202 | 123 | 3 | 12 | 209 | 121 | 322 | 4.78e-19 | 84.7 |
| MS.gene071648.t1 | ATCG01010 | 30.857 | 175 | 108 | 6 | 17 | 181 | 246 | 417 | 4.71e-11 | 62.0 |
Find 0 sgRNAs with CRISPR-Local
Find 80 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTAGCAAAAATAGAAATTTT+AGG | - | 57116:4950-4969 | MS.gene071648:intergenic | 15.0% |
| ! | AATCTGCTATATATTTGACA+CGG | - | 57116:5252-5271 | MS.gene071648:intergenic | 25.0% |
| ! | ATATAGAGTCAAAAGAGATA+TGG | - | 57116:4864-4883 | MS.gene071648:intergenic | 25.0% |
| ! | GTCAAATATATAGCAGATTT+GGG | + | 57116:5253-5272 | MS.gene071648:CDS | 25.0% |
| ! | TGTCAAATATATAGCAGATT+TGG | + | 57116:5252-5271 | MS.gene071648:CDS | 25.0% |
| !! | ATTTCACGTGTTTCTATTTA+TGG | + | 57116:4971-4990 | MS.gene071648:CDS | 25.0% |
| !!! | TCTTTTGACTCTATATATGT+GGG | + | 57116:4868-4887 | MS.gene071648:CDS | 25.0% |
| ATCTGCTATATATTTGACAC+GGG | - | 57116:5251-5270 | MS.gene071648:intergenic | 30.0% | |
| ATGTAGGTTATATTCGTACT+GGG | + | 57116:5119-5138 | MS.gene071648:CDS | 30.0% | |
| ATTACTATGTTCTCATACGT+AGG | + | 57116:5319-5338 | MS.gene071648:CDS | 30.0% | |
| GGAATTCAATCACTACTAAT+TGG | + | 57116:5163-5182 | MS.gene071648:CDS | 30.0% | |
| GTTTCTATTTATGGTTCCTA+TGG | + | 57116:4980-4999 | MS.gene071648:CDS | 30.0% | |
| TACGTATGAGAACATAGTAA+TGG | - | 57116:5320-5339 | MS.gene071648:intergenic | 30.0% | |
| TAGTGATTGAATTCCTTCTA+TGG | - | 57116:5158-5177 | MS.gene071648:intergenic | 30.0% | |
| ! | AAATTTTAGGCGCAATAGAA+AGG | - | 57116:4937-4956 | MS.gene071648:intergenic | 30.0% |
| ! | AGCATTGCTTCTATGATTTT+AGG | + | 57116:5034-5053 | MS.gene071648:CDS | 30.0% |
| ! | AGGTCTTTTTACTTTCGTTT+GGG | - | 57116:5077-5096 | MS.gene071648:intergenic | 30.0% |
| !! | ATAGAATTTGCTACAAAAGC+CGG | - | 57116:5359-5378 | MS.gene071648:intergenic | 30.0% |
| !! | CTCTTTTGACTCTATATATG+TGG | + | 57116:4867-4886 | MS.gene071648:CDS | 30.0% |
| AGCCAAAACGAATCCTATTT+CGG | + | 57116:5282-5301 | MS.gene071648:CDS | 35.0% | |
| ATAGAAAGGAATGCTGTAAC+CGG | - | 57116:4923-4942 | MS.gene071648:intergenic | 35.0% | |
| CATAGTTCAATTGGACATGT+AGG | + | 57116:5103-5122 | MS.gene071648:CDS | 35.0% | |
| CATGTAGGTTATATTCGTAC+TGG | + | 57116:5118-5137 | MS.gene071648:CDS | 35.0% | |
| TTGCCGTAATGCTAAAACTA+TGG | - | 57116:5227-5246 | MS.gene071648:intergenic | 35.0% | |
| ! | GAGGTCTTTTTACTTTCGTT+TGG | - | 57116:5078-5097 | MS.gene071648:intergenic | 35.0% |
| ! | TGAGAACATAGTAATGGAGA+AGG | - | 57116:5314-5333 | MS.gene071648:intergenic | 35.0% |
| !! | TTCTATGATTTTAGGAGCAC+TGG | + | 57116:5042-5061 | MS.gene071648:CDS | 35.0% |
| !!! | TCGCCATAGTTTTAGCATTA+CGG | + | 57116:5221-5240 | MS.gene071648:CDS | 35.0% |
| AAAAGAGATATGGAGTCCGA+AGG | - | 57116:4854-4873 | MS.gene071647:intergenic | 40.0% | |
| AAAGAGATATGGAGTCCGAA+GGG | - | 57116:4853-4872 | MS.gene071647:intergenic | 40.0% | |
| CCAATTGAACTATGAGCTAG+AGG | - | 57116:5097-5116 | MS.gene071648:intergenic | 40.0% | |
| GACTAGCGTTATAGGTTGTT+GGG | + | 57116:5435-5454 | MS.gene071648:CDS | 40.0% | |
| GAGAAGGTTATAGCCGAAAT+AGG | - | 57116:5298-5317 | MS.gene071648:intergenic | 40.0% | |
| TAGAAAGGAATGCTGTAACC+GGG | - | 57116:4922-4941 | MS.gene071648:intergenic | 40.0% | |
| TGACTAGCGTTATAGGTTGT+TGG | + | 57116:5434-5453 | MS.gene071648:CDS | 40.0% | |
| TTCTCATGTGGAACCATAGA+AGG | + | 57116:5142-5161 | MS.gene071648:CDS | 40.0% | |
| TTGTTGCAATGTAGCTCCAT+AGG | - | 57116:4999-5018 | MS.gene071648:intergenic | 40.0% | |
| ! | AGCCGAAATAGGATTCGTTT+TGG | - | 57116:5287-5306 | MS.gene071648:intergenic | 40.0% |
| ! | CCTCTAGCTCATAGTTCAAT+TGG | + | 57116:5094-5113 | MS.gene071648:CDS | 40.0% |
| ! | TATTTGTTCTTCGCCGCTTT+GGG | + | 57116:5376-5395 | MS.gene071648:CDS | 40.0% |
| ! | TTTTACTTTCGTTTGGGCCA+TGG | - | 57116:5071-5090 | MS.gene071648:intergenic | 40.0% |
| AAGAGATATGGAGTCCGAAG+GGG | - | 57116:4852-4871 | MS.gene071647:intergenic | 45.0% | |
| AGAAAGGAATGCTGTAACCG+GGG | - | 57116:4921-4940 | MS.gene071648:intergenic | 45.0% | |
| ATTCGTACTGGGTTCTCATG+TGG | + | 57116:5130-5149 | MS.gene071648:CDS | 45.0% | |
| CTATTTGTTCTTCGCCGCTT+TGG | + | 57116:5375-5394 | MS.gene071648:CDS | 45.0% | |
| GGAGTAGTGACTAGCGTTAT+AGG | + | 57116:5427-5446 | MS.gene071648:CDS | 45.0% | |
| GGTGAACCCTCATAGATATC+AGG | - | 57116:4896-4915 | MS.gene071648:intergenic | 45.0% | |
| TAGCGTTATAGGTTGTTGGG+CGG | + | 57116:5438-5457 | MS.gene071648:CDS | 45.0% | |
| TGGGCACCTGATATCTATGA+GGG | + | 57116:4887-4906 | MS.gene071648:CDS | 45.0% | |
| TTGCTACAAAAGCCGGCTAA+CGG | - | 57116:5352-5371 | MS.gene071648:intergenic | 45.0% | |
| AATAAAACCCCCCTCCCCTT+CGG | + | 57116:4835-4854 | MS.gene071648:CDS | 50.0% | |
| GTGGGCACCTGATATCTATG+AGG | + | 57116:4886-4905 | MS.gene071648:CDS | 50.0% | |
| TACTTTCGTTTGGGCCATGG+CGG | - | 57116:5068-5087 | MS.gene071648:intergenic | 50.0% | |
| TGCTACAAAAGCCGGCTAAC+GGG | - | 57116:5351-5370 | MS.gene071648:intergenic | 50.0% | |
| !!! | AAGGGGAGGGGGGTTTTATT+CGG | - | 57116:4835-4854 | MS.gene071648:intergenic | 50.0% |
| AAGGAATGCTGTAACCGGGG+TGG | - | 57116:4918-4937 | MS.gene071647:intergenic | 55.0% | |
| ACGCTAGTCACTACTCCCAC+TGG | - | 57116:5424-5443 | MS.gene071648:intergenic | 55.0% | |
| AGATATGGAGTCCGAAGGGG+AGG | - | 57116:4849-4868 | MS.gene071648:intergenic | 55.0% | |
| AGGAATGCTGTAACCGGGGT+GGG | - | 57116:4917-4936 | MS.gene071648:intergenic | 55.0% | |
| ATATGGAGTCCGAAGGGGAG+GGG | - | 57116:4847-4866 | MS.gene071648:intergenic | 55.0% | |
| CGCTAGTCACTACTCCCACT+GGG | - | 57116:5423-5442 | MS.gene071648:intergenic | 55.0% | |
| CTACAAAAGCCGGCTAACGG+GGG | - | 57116:5349-5368 | MS.gene071648:intergenic | 55.0% | |
| GATATGGAGTCCGAAGGGGA+GGG | - | 57116:4848-4867 | MS.gene071648:intergenic | 55.0% | |
| GCTACAAAAGCCGGCTAACG+GGG | - | 57116:5350-5369 | MS.gene071648:intergenic | 55.0% | |
| GCTAGTCACTACTCCCACTG+GGG | - | 57116:5422-5441 | MS.gene071648:intergenic | 55.0% | |
| GCTTACTTCCTAGCCCCAGT+GGG | + | 57116:5406-5425 | MS.gene071648:CDS | 55.0% | |
| GTAAGCCCCACAACCCAAAG+CGG | - | 57116:5392-5411 | MS.gene071648:intergenic | 55.0% | |
| GTAGGAATACCCCCGTTAGC+CGG | + | 57116:5337-5356 | MS.gene071648:CDS | 55.0% | |
| GTTATAGGTTGTTGGGCGGC+CGG | + | 57116:5442-5461 | MS.gene071648:CDS | 55.0% | |
| !! | TCTTCGCCGCTTTGGGTTGT+GGG | + | 57116:5383-5402 | MS.gene071648:CDS | 55.0% |
| !! | TTCTTCGCCGCTTTGGGTTG+TGG | + | 57116:5382-5401 | MS.gene071648:CDS | 55.0% |
| !!! | AGGGGAGGGGGGTTTTATTC+GGG | - | 57116:4834-4853 | MS.gene071648:intergenic | 55.0% |
| CTATGAGGGTTCACCCACCC+CGG | + | 57116:4901-4920 | MS.gene071648:CDS | 60.0% | |
| GGCTTACTTCCTAGCCCCAG+TGG | + | 57116:5405-5424 | MS.gene071648:CDS | 60.0% | |
| TATGGAGTCCGAAGGGGAGG+GGG | - | 57116:4846-4865 | MS.gene071648:intergenic | 60.0% | |
| TCACTACTCCCACTGGGGCT+AGG | - | 57116:5417-5436 | MS.gene071648:intergenic | 60.0% | |
| !! | CTTCGCCGCTTTGGGTTGTG+GGG | + | 57116:5384-5403 | MS.gene071648:CDS | 60.0% |
| !! | TTTAGGAGCACTGGCCGCCA+TGG | + | 57116:5051-5070 | MS.gene071648:CDS | 60.0% |
| !!! | GGGGAGGGGGGTTTTATTCG+GGG | - | 57116:4833-4852 | MS.gene071648:intergenic | 60.0% |
| ATGGAGTCCGAAGGGGAGGG+GGG | - | 57116:4845-4864 | MS.gene071648:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 57116 | gene | 4830 | 5467 | 4830 | ID=MS.gene071648 |
| 57116 | mRNA | 4830 | 5467 | 4830 | ID=MS.gene071648.t1;Parent=MS.gene071648 |
| 57116 | exon | 4830 | 5467 | 4830 | ID=MS.gene071648.t1.exon1;Parent=MS.gene071648.t1 |
| 57116 | CDS | 4830 | 5467 | 4830 | ID=cds.MS.gene071648.t1;Parent=MS.gene071648.t1 |
| Gene Sequence |
| Protein sequence |
