AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene071687


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene071687.t1 MTR_4g059730 93.500 200 13 0 1 200 1 200 1.97e-139 388
MS.gene071687.t1 MTR_1g090060 69.543 197 60 0 4 200 3 199 8.70e-103 296
MS.gene071687.t1 MTR_1g090100 69.072 194 60 0 5 198 3 196 8.35e-99 286
MS.gene071687.t1 MTR_1g090070 65.657 198 67 1 4 200 9 206 2.46e-95 277
MS.gene071687.t1 MTR_1g090090 66.497 197 66 0 4 200 3 199 2.94e-94 274
MS.gene071687.t1 MTR_1g115195 49.010 202 99 3 1 200 1 200 2.39e-61 191
MS.gene071687.t1 MTR_1g090150 47.959 196 101 1 3 198 2 196 1.80e-60 189
MS.gene071687.t1 MTR_4g019790 45.960 198 103 2 4 200 7 201 1.96e-57 181
MS.gene071687.t1 MTR_4g019780 46.231 199 103 3 3 200 7 202 2.62e-55 175
MS.gene071687.t1 MTR_7g065740 44.500 200 106 2 3 200 6 202 8.91e-55 174
MS.gene071687.t1 MTR_7g065660 46.231 199 103 2 3 200 6 201 1.47e-54 174
MS.gene071687.t1 MTR_7g065230 45.729 199 99 3 3 200 6 196 1.94e-53 171
MS.gene071687.t1 MTR_3g099757 45.226 199 105 3 3 200 4 199 6.56e-52 167
MS.gene071687.t1 MTR_7g065720 44.000 200 107 2 3 200 6 202 7.84e-52 167
MS.gene071687.t1 MTR_8g087425 46.269 201 102 2 2 200 3 199 3.09e-51 165
MS.gene071687.t1 MTR_7g065750 42.000 200 111 2 3 200 6 202 7.23e-51 164
MS.gene071687.t1 MTR_8g087410 48.792 207 95 6 1 202 2 202 1.25e-50 164
MS.gene071687.t1 MTR_2g070120 45.503 189 98 3 1 188 1 185 1.82e-50 163
MS.gene071687.t1 MTR_5g040430 43.204 206 111 4 2 202 5 209 4.44e-50 162
MS.gene071687.t1 MTR_7g065590 41.000 200 112 3 3 200 6 201 2.50e-49 160
MS.gene071687.t1 MTR_6g080440 46.893 177 88 3 18 192 17 189 1.32e-48 158
MS.gene071687.t1 MTR_7g065710 42.857 203 106 4 1 201 1 195 3.00e-48 157
MS.gene071687.t1 MTR_2g070150 45.050 202 104 4 1 200 1 197 5.26e-48 156
MS.gene071687.t1 MTR_7g065270 42.000 200 110 4 3 200 6 201 6.31e-48 157
MS.gene071687.t1 MTR_7g065600 39.698 199 116 2 3 200 6 201 1.66e-47 155
MS.gene071687.t1 MTR_7g065630 41.500 200 109 3 3 201 24 216 1.91e-47 156
MS.gene071687.t1 MTR_2g070200 44.175 206 107 5 1 203 1 201 3.01e-47 155
MS.gene071687.t1 MTR_7g065700 43.069 202 107 3 1 201 4 198 3.58e-47 155
MS.gene071687.t1 MTR_8g061950 44.776 201 103 6 5 198 6 205 3.64e-47 155
MS.gene071687.t1 MTR_7g065640 41.709 199 94 3 3 200 6 183 9.49e-46 150
MS.gene071687.t1 MTR_7g065260 43.564 202 108 4 3 202 13 210 1.07e-45 151
MS.gene071687.t1 MTR_2g070130 43.386 189 102 3 1 188 1 185 5.19e-45 149
MS.gene071687.t1 MTR_7g065290 39.196 199 117 2 3 200 6 201 1.04e-44 148
MS.gene071687.t1 MTR_7g065265 40.201 199 115 2 3 200 6 201 2.58e-44 147
MS.gene071687.t1 MTR_2g070180 49.057 159 78 2 1 158 1 157 3.02e-44 147
MS.gene071687.t1 MTR_8g056940 42.564 195 107 3 1 194 1 191 1.38e-42 143
MS.gene071687.t1 MTR_2g070210 46.584 161 81 3 1 158 1 159 1.57e-42 142
MS.gene071687.t1 MTR_4g124130 44.737 190 99 4 1 188 1 186 5.83e-42 141
MS.gene071687.t1 MTR_7g065680 40.500 200 111 3 3 201 6 198 7.69e-42 141
MS.gene071687.t1 MTR_2g070140 44.086 186 97 4 17 200 1 181 3.87e-41 139
MS.gene071687.t1 MTR_3g467420 43.889 180 96 3 10 188 15 190 5.89e-41 139
MS.gene071687.t1 MTR_5g037380 38.350 206 121 3 3 202 4 209 2.15e-40 137
MS.gene071687.t1 MTR_3g467430 42.781 187 102 3 3 188 8 190 6.97e-40 136
MS.gene071687.t1 MTR_2g070070 42.857 189 103 3 1 188 1 185 3.13e-39 134
MS.gene071687.t1 MTR_2g070060 41.270 189 106 3 1 188 1 185 9.11e-39 133
MS.gene071687.t1 MTR_2g070110 43.455 191 98 5 1 188 1 184 1.13e-37 130
MS.gene071687.t1 MTR_0186s0030 46.324 136 72 1 1 135 1 136 1.10e-35 122
MS.gene071687.t1 MTR_5g076900 37.766 188 112 3 2 188 4 187 4.78e-35 123
MS.gene071687.t1 MTR_2g072120 60.714 56 21 1 48 102 2 57 2.27e-19 79.0
MS.gene071687.t1 MTR_5g037390 42.857 91 42 2 28 115 11 94 3.68e-13 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene071687.t1 AT2G29450 46.465 198 102 3 3 198 5 200 4.30e-54 172
MS.gene071687.t1 AT3G09270 44.554 202 108 3 3 202 5 204 6.78e-54 172
MS.gene071687.t1 AT2G29490 45.685 197 104 2 3 198 6 200 3.98e-53 170
MS.gene071687.t1 AT1G10370 45.050 202 106 3 2 198 3 204 8.90e-52 167
MS.gene071687.t1 AT2G29460 44.221 199 108 2 3 200 6 202 1.42e-51 166
MS.gene071687.t1 AT2G29420 42.640 197 111 1 2 198 7 201 1.48e-51 166
MS.gene071687.t1 AT2G29470 43.500 200 109 3 3 200 6 203 2.62e-50 163
MS.gene071687.t1 AT2G29480 44.221 199 108 2 3 200 6 202 2.44e-49 160
MS.gene071687.t1 AT1G78340 45.198 177 94 2 1 176 1 175 5.50e-46 151
MS.gene071687.t1 AT1G10360 42.439 205 113 3 3 202 4 208 5.66e-46 152
MS.gene071687.t1 AT2G29440 41.117 197 113 2 3 198 5 199 6.69e-46 151
MS.gene071687.t1 AT1G74590 40.099 202 118 2 3 201 6 207 6.05e-45 149
MS.gene071687.t1 AT1G27130 42.132 197 108 4 3 193 5 201 6.18e-45 149
MS.gene071687.t1 AT1G17180 43.386 189 102 3 1 188 1 185 2.40e-44 147
MS.gene071687.t1 AT1G27140 42.929 198 106 4 3 193 5 202 1.98e-43 145
MS.gene071687.t1 AT1G78320 42.268 194 103 6 1 191 1 188 4.10e-43 144
MS.gene071687.t1 AT5G62480 37.264 212 118 5 1 201 5 212 7.84e-43 144
MS.gene071687.t1 AT1G59670 41.837 196 108 4 3 192 5 200 1.38e-42 143
MS.gene071687.t1 AT1G69930 38.660 194 115 3 3 192 11 204 9.78e-42 141
MS.gene071687.t1 AT1G78370 41.270 189 106 3 1 188 1 185 3.76e-41 139
MS.gene071687.t1 AT1G17170 45.912 159 82 3 1 158 1 156 1.63e-40 137
MS.gene071687.t1 AT1G59700 39.286 196 113 4 3 192 5 200 1.90e-40 138
MS.gene071687.t1 AT1G17190 39.572 187 108 3 3 188 4 186 8.13e-40 135
MS.gene071687.t1 AT1G78380 42.328 189 104 3 1 188 1 185 1.08e-39 135
MS.gene071687.t1 AT1G69920 37.436 195 116 4 5 193 35 229 2.96e-37 130
MS.gene071687.t1 AT1G53680 40.110 182 102 4 10 188 13 190 4.82e-36 126
MS.gene071687.t1 AT1G78360 43.750 160 86 3 1 158 1 158 1.40e-35 125
MS.gene071687.t1 AT3G43800 38.916 203 115 5 3 200 4 202 2.95e-34 122
MS.gene071687.t1 AT5G62480 30.622 209 105 5 3 201 7 185 8.19e-27 102
MS.gene071687.t1 AT5G62480 30.622 209 106 5 3 201 7 186 8.84e-27 102
MS.gene071687.t1 AT1G78320 34.211 190 91 6 1 188 1 158 1.40e-24 95.9

Find 38 sgRNAs with CRISPR-Local

Find 58 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGAGAGCCCAAGCTCGGTTC+TGG 0.280479 4.4:-44444305 MS.gene071687:CDS
AGGAGACACCGTTAACATTC+TGG 0.299490 4.4:-44443825 MS.gene071687:CDS
AAGTGAAGCTACATGGATTT+TGG 0.302285 4.4:-44444563 MS.gene071687:CDS
AATACTACCCTCAAAGTAAA+AGG 0.302524 4.4:+44444534 None:intergenic
AGAATACCATAGCGAAAATC+TGG 0.331408 4.4:-44443904 MS.gene071687:CDS
GTATTGACCCTTAAGCTAAA+AGG 0.363440 4.4:-44444516 MS.gene071687:CDS
TTGAATACATTGATGAGATA+TGG 0.380718 4.4:-44444362 MS.gene071687:CDS
TCTTCTTCTATGTTCTCATA+TGG 0.416671 4.4:+44444489 None:intergenic
AACATTCTGGACATAGCTCT+TGG 0.422694 4.4:-44443812 MS.gene071687:CDS
AAAACTCCGGTGTTGGTTCA+TGG 0.434187 4.4:-44444417 MS.gene071687:CDS
AGCTATGTCCAGAATGTTAA+CGG 0.440075 4.4:+44443817 None:intergenic
GCTATCAGTTTCTCATGATC+AGG 0.440168 4.4:+44443660 None:intergenic
GCTCTCTCATAAGGATCAAC+AGG 0.442610 4.4:+44444321 None:intergenic
CGAGCTTGGGCTCTCTCATA+AGG 0.459155 4.4:+44444312 None:intergenic
AATTCCCTCACTTGCATGCA+TGG 0.474769 4.4:-44443724 MS.gene071687:CDS
TTCAAGAATGTACCTGTCAT+AGG 0.475029 4.4:-44443692 MS.gene071687:CDS
GAGAGCCCAAGCTCGGTTCT+GGG 0.483641 4.4:-44444304 MS.gene071687:CDS
GATTGTATTGAAGAAGTTGA+GGG 0.534705 4.4:+44444451 None:intergenic
TCAAACCATGCATGCAAGTG+AGG 0.535813 4.4:+44443719 None:intergenic
TAAGGATCAACAGGAAGCAA+AGG 0.547066 4.4:+44444330 None:intergenic
AAGGATCAACAGGAAGCAAA+GGG 0.553758 4.4:+44444331 None:intergenic
GCAGATCAAGTGAAGCTACA+TGG 0.559561 4.4:-44444570 MS.gene071687:CDS
GAGGGAATTTCTCAGCTGCT+AGG 0.567585 4.4:+44443738 None:intergenic
CAAACCATGCATGCAAGTGA+GGG 0.568010 4.4:+44443720 None:intergenic
GGATTGTATTGAAGAAGTTG+AGG 0.571991 4.4:+44444450 None:intergenic
GCACAAGAAAACTCCGGTGT+TGG 0.576241 4.4:-44444424 MS.gene071687:CDS
CTAACGAGTGCACCAACTGC+AGG 0.579048 4.4:+44443950 None:intergenic
GAATACCATAGCGAAAATCT+GGG 0.582158 4.4:-44443903 MS.gene071687:CDS
AGGAAGATTTCACCTATGAC+AGG 0.599534 4.4:+44443680 None:intergenic
GGTGCACTCGTTAGTAGCGG+TGG 0.611355 4.4:-44443941 MS.gene071687:CDS
CTTATGAGAGAGCCCAAGCT+CGG 0.617875 4.4:-44444311 MS.gene071687:CDS
TTTCCACCATGAACCAACAC+CGG 0.639081 4.4:+44444411 None:intergenic
ATGATCATAGACTCACATAG+AGG 0.640551 4.4:+44444387 None:intergenic
GTAGCGGTGGCGATGAAGAG+CGG 0.649339 4.4:-44443928 MS.gene071687:CDS
TCCAGTGCACAAGAAAACTC+CGG 0.653047 4.4:-44444430 MS.gene071687:CDS
ACTCCGGTGTTGGTTCATGG+TGG 0.663999 4.4:-44444414 MS.gene071687:CDS
GTTGGTGCACTCGTTAGTAG+CGG 0.664719 4.4:-44443944 MS.gene071687:CDS
ACAGGAAGCAAAGGGTACTG+TGG 0.666663 4.4:+44444339 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTTAATCAACATATTATTT+AGG - chr4.4:44444086-44444105 MS.gene071687:intron 10.0%
!! TTATCATATTGAATAAATTT+AGG + chr4.4:44444223-44444242 None:intergenic 10.0%
!! AATAATAATATTGTTGGATA+TGG + chr4.4:44444163-44444182 None:intergenic 15.0%
!!! AAATTACTATTACTTTTTCA+TGG + chr4.4:44444023-44444042 None:intergenic 15.0%
! CTAAGAAAATTTGCAAATGA+AGG + chr4.4:44444125-44444144 None:intergenic 25.0%
! TTGAATACATTGATGAGATA+TGG - chr4.4:44443875-44443894 MS.gene071687:CDS 25.0%
AATACTACCCTCAAAGTAAA+AGG + chr4.4:44443706-44443725 None:intergenic 30.0%
GATTGTATTGAAGAAGTTGA+GGG + chr4.4:44443789-44443808 None:intergenic 30.0%
TCTTCTTCTATGTTCTCATA+TGG + chr4.4:44443751-44443770 None:intergenic 30.0%
! GACTACAAGAAGAAACTTTT+TGG - chr4.4:44444389-44444408 MS.gene071687:CDS 30.0%
! TACAAGAAGAAACTTTTTGG+AGG - chr4.4:44444392-44444411 MS.gene071687:CDS 30.0%
! TTTTCATGGTATGCAAATGA+AGG + chr4.4:44444009-44444028 None:intergenic 30.0%
! TTTTTATAGATTCCTGCAGT+TGG - chr4.4:44444275-44444294 MS.gene071687:intron 30.0%
!!! ATGGTATACCTTTTAGCTTA+AGG + chr4.4:44443732-44443751 None:intergenic 30.0%
!!! TGGTATACCTTTTAGCTTAA+GGG + chr4.4:44443731-44443750 None:intergenic 30.0%
AGAATACCATAGCGAAAATC+TGG - chr4.4:44444333-44444352 MS.gene071687:CDS 35.0%
AGCTATGTCCAGAATGTTAA+CGG + chr4.4:44444423-44444442 None:intergenic 35.0%
ATGATCATAGACTCACATAG+AGG + chr4.4:44443853-44443872 None:intergenic 35.0%
GAATACCATAGCGAAAATCT+GGG - chr4.4:44444334-44444353 MS.gene071687:CDS 35.0%
GGATATGGTATCAACTATCA+AGG + chr4.4:44444148-44444167 None:intergenic 35.0%
GGATTGTATTGAAGAAGTTG+AGG + chr4.4:44443790-44443809 None:intergenic 35.0%
TTCAAGAATGTACCTGTCAT+AGG - chr4.4:44444545-44444564 MS.gene071687:CDS 35.0%
! AAGTGAAGCTACATGGATTT+TGG - chr4.4:44443674-44443693 MS.gene071687:CDS 35.0%
! GTACAGTCCTTTTACTTTGA+GGG - chr4.4:44443696-44443715 MS.gene071687:CDS 35.0%
! GTATTGACCCTTAAGCTAAA+AGG - chr4.4:44443721-44443740 MS.gene071687:CDS 35.0%
AACATTCTGGACATAGCTCT+TGG - chr4.4:44444425-44444444 MS.gene071687:CDS 40.0%
AAGGATCAACAGGAAGCAAA+GGG + chr4.4:44443909-44443928 None:intergenic 40.0%
AGGAAGATTTCACCTATGAC+AGG + chr4.4:44444560-44444579 None:intergenic 40.0%
TAAGGATCAACAGGAAGCAA+AGG + chr4.4:44443910-44443929 None:intergenic 40.0%
! ATGTTCCCAGATTTTCGCTA+TGG + chr4.4:44444342-44444361 None:intergenic 40.0%
! GGTACAGTCCTTTTACTTTG+AGG - chr4.4:44443695-44443714 MS.gene071687:CDS 40.0%
AATTCCCTCACTTGCATGCA+TGG - chr4.4:44444513-44444532 MS.gene071687:CDS 45.0%
AGGAGACACCGTTAACATTC+TGG - chr4.4:44444412-44444431 MS.gene071687:CDS 45.0%
CAAACCATGCATGCAAGTGA+GGG + chr4.4:44444520-44444539 None:intergenic 45.0%
GCAGATCAAGTGAAGCTACA+TGG - chr4.4:44443667-44443686 MS.gene071687:CDS 45.0%
GCTCTCTCATAAGGATCAAC+AGG + chr4.4:44443919-44443938 None:intergenic 45.0%
TCAAACCATGCATGCAAGTG+AGG + chr4.4:44444521-44444540 None:intergenic 45.0%
TCCAGTGCACAAGAAAACTC+CGG - chr4.4:44443807-44443826 MS.gene071687:CDS 45.0%
TTTCCACCATGAACCAACAC+CGG + chr4.4:44443829-44443848 None:intergenic 45.0%
! ATTTTACCCAGAACCGAGCT+TGG + chr4.4:44443942-44443961 None:intergenic 45.0%
! TTTTACCCAGAACCGAGCTT+GGG + chr4.4:44443941-44443960 None:intergenic 45.0%
!! AAAACTCCGGTGTTGGTTCA+TGG - chr4.4:44443820-44443839 MS.gene071687:CDS 45.0%
!! AATAATAATAATAATATTGT+TGG + chr4.4:44444169-44444188 None:intergenic 5.0%
!! AGTATTATAAATAATTTATT+GGG - chr4.4:44444248-44444267 MS.gene071687:intron 5.0%
!! TAGTATTATAAATAATTTAT+TGG - chr4.4:44444247-44444266 MS.gene071687:intron 5.0%
CTTATGAGAGAGCCCAAGCT+CGG - chr4.4:44443926-44443945 MS.gene071687:CDS 50.0%
GAGGGAATTTCTCAGCTGCT+AGG + chr4.4:44444502-44444521 None:intergenic 50.0%
GCACAAGAAAACTCCGGTGT+TGG - chr4.4:44443813-44443832 MS.gene071687:CDS 50.0%
GTTGGTGCACTCGTTAGTAG+CGG - chr4.4:44444293-44444312 MS.gene071687:CDS 50.0%
! ACCGGAGTTTTCTTGTGCAC+TGG + chr4.4:44443811-44443830 None:intergenic 50.0%
!! ACAGGAAGCAAAGGGTACTG+TGG + chr4.4:44443901-44443920 None:intergenic 50.0%
CGAGCTTGGGCTCTCTCATA+AGG + chr4.4:44443928-44443947 None:intergenic 55.0%
CTAACGAGTGCACCAACTGC+AGG + chr4.4:44444290-44444309 None:intergenic 55.0%
!! ACTCCGGTGTTGGTTCATGG+TGG - chr4.4:44443823-44443842 MS.gene071687:CDS 55.0%
AGAGAGCCCAAGCTCGGTTC+TGG - chr4.4:44443932-44443951 MS.gene071687:CDS 60.0%
GAGAGCCCAAGCTCGGTTCT+GGG - chr4.4:44443933-44443952 MS.gene071687:CDS 60.0%
GGTGCACTCGTTAGTAGCGG+TGG - chr4.4:44444296-44444315 MS.gene071687:CDS 60.0%
GTAGCGGTGGCGATGAAGAG+CGG - chr4.4:44444309-44444328 MS.gene071687:CDS 60.0%


Chromosome Type Strat End Strand Name
chr4.4 gene 44443664 44444595 44443664 ID=MS.gene071687
chr4.4 mRNA 44443664 44444595 44443664 ID=MS.gene071687.t1;Parent=MS.gene071687
chr4.4 exon 44444284 44444595 44444284 ID=MS.gene071687.t1.exon1;Parent=MS.gene071687.t1
chr4.4 CDS 44444284 44444595 44444284 ID=cds.MS.gene071687.t1;Parent=MS.gene071687.t1
chr4.4 exon 44443664 44443975 44443664 ID=MS.gene071687.t1.exon2;Parent=MS.gene071687.t1
chr4.4 CDS 44443664 44443975 44443664 ID=cds.MS.gene071687.t1;Parent=MS.gene071687.t1
Gene Sequence

>MS.gene071687

ATGGCAGATCAAGTGAAGCTACATGGATTTTGGTACAGTCCTTTTACTTTGAGGGTAGTATTGACCCTTAAGCTAAAAGGTATACCATATGAGAACATAGAAGAAGATCGTTTCAACAAGAGCCCTCAACTTCTTCAATACAATCCAGTGCACAAGAAAACTCCGGTGTTGGTTCATGGTGGAAAACCTCTATGTGAGTCTATGATCATTGTTGAATACATTGATGAGATATGGCCACAGTACCCTTTGCTTCCTGTTGATCCTTATGAGAGAGCCCAAGCTCGGTTCTGGGTAAAATATGTCGATGACATTGTAAGTTTAATCATGTGCAAATTTTCTTAGCCTTCATTTGCATACCATGAAAAAGTAATAGTAATTTTATTCATATTATTCAATCATTTAATCAAACATATTATTTAATCATTTAATCAACATATTATTTAGGCAAATTTTCATAGCCTTCATTTGCAAATTTTCTTAGCCTTGATAGTTGATACCATATCCAACAATATTATTATTATTATTTTTTTTTTATTTTTTTTTTTTTATAGATTTGCCTAAATTTATTCAATATGATAAATAATAGTATTATAAATAATTTATTGGGTCAATTTTTATAGATTCCTGCAGTTGGTGCACTCGTTAGTAGCGGTGGCGATGAAGAGCGGAAGAATACCATAGCGAAAATCTGGGAACATCTTAGAGTTGTTGAAGAACAATGCATTGACTACAAGAAGAAACTTTTTGGAGGAGACACCGTTAACATTCTGGACATAGCTCTTGGATCAACTATCAAGTTTCTTGTAACTATTGAAGATATCATTGAATTTAAGATCCTAGCAGCTGAGAAATTCCCTCACTTGCATGCATGGTTTGAAAATTTCAAGAATGTACCTGTCATAGGTGAAATCTTCCTGATCATGAGAAACTGA

Protein sequence

>MS.gene071687.t1

MADQVKLHGFWYSPFTLRVVLTLKLKGIPYENIEEDRFNKSPQLLQYNPVHKKTPVLVHGGKPLCESMIIVEYIDEIWPQYPLLPVDPYERAQARFWVKYVDDIIPAVGALVSSGGDEERKNTIAKIWEHLRVVEEQCIDYKKKLFGGDTVNILDIALGSTIKFLVTIEDIIEFKILAAEKFPHLHAWFENFKNVPVIGEIFLIMRN