AlfalfaGEDB | MS.gene072081

M. sativa cultivar XinJiangDaYe / MS.gene072081

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene072081.t1	XP_013462379.1	88.8	161	11	1	1	154	1	161	6.50E-72	280

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene072081.t1	Q1SKW7	88.8	161	11	1	1	154	1	161	4.7e-72	280.0

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene051648	MS.gene072081	0.821708	3.50E-53	-1.69E-46
MS.gene054999	MS.gene072081	0.826421	2.74E-54	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene072081.t1	MTR_2g005740	93.789	161	3	1	1	154	1	161	1.33e-108	306
MS.gene072081.t1	MTR_2g005740	93.377	151	3	1	11	154	1	151	1.55e-100	285
MS.gene072081.t1	MTR_2g012780	45.714	105	48	2	47	148	53	151	5.10e-24	92.0
MS.gene072081.t1	MTR_4g090440	45.833	120	46	5	38	149	25	133	4.43e-23	89.0

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene072081.t1	AT1G09812	46.715	137	59	3	8	144	3	125	1.39e-29	105
MS.gene072081.t1	AT1G58007	48.571	105	44	3	42	146	20	114	1.59e-23	89.4
MS.gene072081.t1	AT1G58007	48.571	105	44	3	42	146	20	114	1.59e-23	89.4
MS.gene072081.t1	AT1G58007	48.571	105	44	3	42	146	20	114	1.59e-23	89.4
MS.gene072081.t1	AT1G11120	46.341	123	58	4	32	147	24	145	2.00e-22	87.8
MS.gene072081.t1	AT1G11120	39.597	149	58	5	31	147	23	171	2.20e-19	80.5
MS.gene072081.t1	AT4G28170	36.207	116	52	6	23	133	37	135	8.71e-12	59.7
MS.gene072081.t1	AT1G58007	81.250	32	6	0	42	73	20	51	1.74e-11	56.6

Find 42 sgRNAs with CRISPR-Local

Find 51 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GATTTCAATGTTTCTAATAA+TGG	0.253747	2.4:+76409275	MS.gene072081:CDS
TTTGAACAAATGTTCAAATA+TGG	0.259962	2.4:-76409298	None:intergenic
GTCCAAGGGAAGAAGCTTAT+TGG	0.267181	2.4:+76409590	MS.gene072081:CDS
TAGAAACATTGAAATCAAAA+TGG	0.315037	2.4:-76409268	None:intergenic
ATCTATGCTTCCAACTTATC+AGG	0.349676	2.4:+76409350	MS.gene072081:CDS
TCTATGAGTGCAATATCATT+TGG	0.374376	2.4:+76409231	MS.gene072081:CDS
GAAGGTCCATGGAAAAGCTT+TGG	0.389743	2.4:+76409204	MS.gene072081:CDS
GGCCAATAAGCTTCTTCCCT+TGG	0.399066	2.4:-76409592	None:intergenic
GTCTTATGCATGTGATTTGT+CGG	0.403359	2.4:+76409510	MS.gene072081:CDS
TGATTTGTCGGTGTTGATGC+CGG	0.444835	2.4:+76409522	MS.gene072081:CDS
TGTTAAATACAAAATGTTGT+TGG	0.457080	2.4:-76409621	None:intergenic
CTTGGACAAGGCAGAGGAGC+AGG	0.461138	2.4:-76409574	None:intergenic
ACAAGGCAGAGGAGCAGGTT+GGG	0.462248	2.4:-76409569	None:intergenic
GATTTGTCGGTGTTGATGCC+GGG	0.464814	2.4:+76409523	MS.gene072081:CDS
TCTTCCCTTGGACAAGGCAG+AGG	0.486050	2.4:-76409580	None:intergenic
AAGGTCCATGGAAAAGCTTT+GGG	0.488752	2.4:+76409205	MS.gene072081:CDS
TAAGTTGGAAGCATAGATTC+TGG	0.501581	2.4:-76409345	None:intergenic
AGGAGGAGGAGGAGTTGATG+AGG	0.501669	2.4:+76409182	MS.gene072081:CDS
CTGCTCCTCTGCCTTGTCCA+AGG	0.512022	2.4:+76409575	MS.gene072081:CDS
CCTCCTGTCTGTGAATGTGA+TGG	0.513542	2.4:-76409165	None:intergenic
ACAAAATGTTGTTGGTGAGA+AGG	0.517710	2.4:-76409613	None:intergenic
TGCTCCTCTGCCTTGTCCAA+GGG	0.530867	2.4:+76409576	MS.gene072081:CDS
TAAGTAGGATATTGCTGCCC+CGG	0.537196	2.4:-76409541	None:intergenic
GACAAGGCAGAGGAGCAGGT+TGG	0.540635	2.4:-76409570	None:intergenic
GTATACACGGCTTCAAACGC+AGG	0.541704	2.4:+76409141	MS.gene072081:CDS
GAGTTGATGAGGAAGGTCCA+TGG	0.553113	2.4:+76409193	MS.gene072081:CDS
CATTGAAATCAAAATGGCTG+TGG	0.558224	2.4:-76409262	None:intergenic
CATTCACAGACAGGAGGAGG+AGG	0.558241	2.4:+76409171	MS.gene072081:CDS
GAGTGGTGTTCCTGATAAGT+TGG	0.558569	2.4:-76409360	None:intergenic
GCAGGTTGGGCAATGTAAGT+AGG	0.571068	2.4:-76409556	None:intergenic
GGTCCATCACATTCACAGAC+AGG	0.587985	2.4:+76409162	MS.gene072081:CDS
TCACATTCACAGACAGGAGG+AGG	0.591315	2.4:+76409168	MS.gene072081:CDS
GGAGGAGGAGTTGATGAGGA+AGG	0.605051	2.4:+76409186	MS.gene072081:CDS
TAAGCTTCTTCCCTTGGACA+AGG	0.606151	2.4:-76409586	None:intergenic
TCACTACCAACCACAAAACA+AGG	0.611869	2.4:+76409381	MS.gene072081:CDS
GTGGAAGAAAAGTGTTGTGT+GGG	0.618638	2.4:-76409321	None:intergenic
ACAGTGAAAGATCAAAGATG+TGG	0.629582	2.4:+76409115	MS.gene072081:CDS
CGTGGAAGAAAAGTGTTGTG+TGG	0.639502	2.4:-76409322	None:intergenic
ATTTGTCGGTGTTGATGCCG+GGG	0.648111	2.4:+76409524	MS.gene072081:CDS
AAAGATGTGGAACGTATACA+CGG	0.657288	2.4:+76409128	MS.gene072081:CDS
TGGAAGCATAGATTCTGGCG+TGG	0.678051	2.4:-76409340	None:intergenic
CCATCACATTCACAGACAGG+AGG	0.694212	2.4:+76409165	MS.gene072081:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	CATTATACATATGTTAAAAA+AGG	-	chr2.4:76409456-76409475	None:intergenic	15.0%
!!	GTATGTTTATTTATTATTAC+AGG	+	chr2.4:76409477-76409496	MS.gene072081:intron	15.0%
!!	TAGAAACATTGAAATCAAAA+TGG	-	chr2.4:76409271-76409290	None:intergenic	20.0%
!!	TGTTAAATACAAAATGTTGT+TGG	-	chr2.4:76409624-76409643	None:intergenic	20.0%
!!	TTTGAACAAATGTTCAAATA+TGG	-	chr2.4:76409301-76409320	None:intergenic	20.0%
!!!	GATTTCAATGTTTCTAATAA+TGG	+	chr2.4:76409275-76409294	MS.gene072081:CDS	20.0%
	AAGGAAAAAACACATGAAAC+AGG	-	chr2.4:76409437-76409456	None:intergenic	30.0%
	ACATGAAACAGGTAGTAAAA+GGG	-	chr2.4:76409426-76409445	None:intergenic	30.0%
	TCTATGAGTGCAATATCATT+TGG	+	chr2.4:76409231-76409250	MS.gene072081:CDS	30.0%
	AAAGATGTGGAACGTATACA+CGG	+	chr2.4:76409128-76409147	MS.gene072081:CDS	35.0%
	ACAGTGAAAGATCAAAGATG+TGG	+	chr2.4:76409115-76409134	MS.gene072081:CDS	35.0%
	ATCTATGCTTCCAACTTATC+AGG	+	chr2.4:76409350-76409369	MS.gene072081:CDS	35.0%
	CACATGAAACAGGTAGTAAA+AGG	-	chr2.4:76409427-76409446	None:intergenic	35.0%
	CATTGAAATCAAAATGGCTG+TGG	-	chr2.4:76409265-76409284	None:intergenic	35.0%
!	ACAAAATGTTGTTGGTGAGA+AGG	-	chr2.4:76409616-76409635	None:intergenic	35.0%
!	GTCTTATGCATGTGATTTGT+CGG	+	chr2.4:76409510-76409529	MS.gene072081:CDS	35.0%
!	TAAGTTGGAAGCATAGATTC+TGG	-	chr2.4:76409348-76409367	None:intergenic	35.0%
!!!	TTGTGCATTTCCTTGTTTTG+TGG	-	chr2.4:76409394-76409413	None:intergenic	35.0%
	AAGGTCCATGGAAAAGCTTT+GGG	+	chr2.4:76409205-76409224	MS.gene072081:CDS	40.0%
	TCACTACCAACCACAAAACA+AGG	+	chr2.4:76409381-76409400	MS.gene072081:CDS	40.0%
!!	GTGGAAGAAAAGTGTTGTGT+GGG	-	chr2.4:76409324-76409343	None:intergenic	40.0%
!!!	GCATTTCCTTGTTTTGTGGT+TGG	-	chr2.4:76409390-76409409	None:intergenic	40.0%
	GAAGGTCCATGGAAAAGCTT+TGG	+	chr2.4:76409204-76409223	MS.gene072081:CDS	45.0%
	GAGTGGTGTTCCTGATAAGT+TGG	-	chr2.4:76409363-76409382	None:intergenic	45.0%
	GTCCAAGGGAAGAAGCTTAT+TGG	+	chr2.4:76409590-76409609	MS.gene072081:CDS	45.0%
	TAAGCTTCTTCCCTTGGACA+AGG	-	chr2.4:76409589-76409608	None:intergenic	45.0%
	TAAGTAGGATATTGCTGCCC+CGG	-	chr2.4:76409544-76409563	None:intergenic	45.0%
!	GAAGTCCCAAAGCTTTTCCA+TGG	-	chr2.4:76409213-76409232	None:intergenic	45.0%
!!	CGTGGAAGAAAAGTGTTGTG+TGG	-	chr2.4:76409325-76409344	None:intergenic	45.0%
!!	TGATTTGTCGGTGTTGATGC+CGG	+	chr2.4:76409522-76409541	MS.gene072081:CDS	45.0%
!!!	GTTTTGTGGTTGGTAGTGAG+TGG	-	chr2.4:76409380-76409399	None:intergenic	45.0%
	CCATCACATTCACAGACAGG+AGG	+	chr2.4:76409165-76409184	MS.gene072081:CDS	50.0%
	CCTCCTGTCTGTGAATGTGA+TGG	-	chr2.4:76409168-76409187	None:intergenic	50.0%
	GGCCAATAAGCTTCTTCCCT+TGG	-	chr2.4:76409595-76409614	None:intergenic	50.0%
	GGTCCATCACATTCACAGAC+AGG	+	chr2.4:76409162-76409181	MS.gene072081:CDS	50.0%
	GTATACACGGCTTCAAACGC+AGG	+	chr2.4:76409141-76409160	MS.gene072081:CDS	50.0%
	TCACATTCACAGACAGGAGG+AGG	+	chr2.4:76409168-76409187	MS.gene072081:CDS	50.0%
!	GCAGGTTGGGCAATGTAAGT+AGG	-	chr2.4:76409559-76409578	None:intergenic	50.0%
!	TGGAAGCATAGATTCTGGCG+TGG	-	chr2.4:76409343-76409362	None:intergenic	50.0%
!!	ATTTGTCGGTGTTGATGCCG+GGG	+	chr2.4:76409524-76409543	MS.gene072081:CDS	50.0%
!!	GAGTTGATGAGGAAGGTCCA+TGG	+	chr2.4:76409193-76409212	MS.gene072081:CDS	50.0%
!!	GATTTGTCGGTGTTGATGCC+GGG	+	chr2.4:76409523-76409542	MS.gene072081:CDS	50.0%
	ACAAGGCAGAGGAGCAGGTT+GGG	-	chr2.4:76409572-76409591	None:intergenic	55.0%
	CATTCACAGACAGGAGGAGG+AGG	+	chr2.4:76409171-76409190	MS.gene072081:CDS	55.0%
	TCTTCCCTTGGACAAGGCAG+AGG	-	chr2.4:76409583-76409602	None:intergenic	55.0%
	TGCTCCTCTGCCTTGTCCAA+GGG	+	chr2.4:76409576-76409595	MS.gene072081:CDS	55.0%
!!	AGGAGGAGGAGGAGTTGATG+AGG	+	chr2.4:76409182-76409201	MS.gene072081:CDS	55.0%
!!	GGAGGAGGAGTTGATGAGGA+AGG	+	chr2.4:76409186-76409205	MS.gene072081:CDS	55.0%
	CTGCTCCTCTGCCTTGTCCA+AGG	+	chr2.4:76409575-76409594	MS.gene072081:CDS	60.0%
	CTTGGACAAGGCAGAGGAGC+AGG	-	chr2.4:76409577-76409596	None:intergenic	60.0%
	GACAAGGCAGAGGAGCAGGT+TGG	-	chr2.4:76409573-76409592	None:intergenic	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.4	gene	76409102	76409648	76409102	ID=MS.gene072081
chr2.4	mRNA	76409102	76409648	76409102	ID=MS.gene072081.t1;Parent=MS.gene072081
chr2.4	exon	76409102	76409416	76409102	ID=MS.gene072081.t1.exon1;Parent=MS.gene072081.t1
chr2.4	CDS	76409102	76409416	76409102	ID=cds.MS.gene072081.t1;Parent=MS.gene072081.t1
chr2.4	exon	76409499	76409648	76409499	ID=MS.gene072081.t1.exon2;Parent=MS.gene072081.t1
chr2.4	CDS	76409499	76409648	76409499	ID=cds.MS.gene072081.t1;Parent=MS.gene072081.t1

Gene Sequence

>MS.gene072081

ATGAGCGATTTAAACAGTGAAAGATCAAAGATGTGGAACGTATACACGGCTTCAAACGCAGGTCCATCACATTCACAGACAGGAGGAGGAGGAGTTGATGAGGAAGGTCCATGGAAAAGCTTTGGGACTTCTATGAGTGCAATATCATTTGGTTTTGTTGCCACAGCCATTTTGATTTCAATGTTTCTAATAATGGCCATATTTGAACATTTGTTCAAACCCACACAACACTTTTCTTCCACGCCAGAATCTATGCTTCCAACTTATCAGGAACACCACTCACTACCAACCACAAAACAAGGAAATGCACAATCAGTAAGTCCCTTTTACTACCTGTTTCATGTGTTTTTTCCTTTTTTAACATATGTATAATGTGTATGTTTATTTATTATTACAGGTTCTATCATCGTCTTATGCATGTGATTTGTCGGTGTTGATGCCGGGGCAGCAATATCCTACTTACATTGCCCAACCTGCTCCTCTGCCTTGTCCAAGGGAAGAAGCTTATTGGCCTTCTCACCAACAACATTTTGTATTTAACAACTAG

Protein sequence

>MS.gene072081.t1

MSDLNSERSKMWNVYTASNAGPSHSQTGGGGVDEEGPWKSFGTSMSAISFGFVATAILISMFLIMAIFEHLFKPTQHFSSTPESMLPTYQEHHSLPTTKQGNAQSVLSSSYACDLSVLMPGQQYPTYIAQPAPLPCPREEAYWPSHQQHFVFNN