AlfalfaGEDB | MS.gene072495

M. sativa cultivar XinJiangDaYe / MS.gene072495

KEGG: -
GO: -
PFAM: PF05498.14

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene072495.t1	XP_003626248.1	100	108	0	0	1	108	19	126	4.60E-56	226.9

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene072495.t1	Q8L9P8	84.2	76	12	0	33	108	40	115	5.2e-32	138.3

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene072495.t1	A2Q327	100.0	108	0	0	1	108	19	126	3.3e-56	226.9

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene050903	MS.gene072495	0.956777	1.32E-114	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene072495.t1	MTR_7g113080	100.000	108	0	0	1	108	19	126	3.21e-79	228
MS.gene072495.t1	MTR_4g119860	60.360	111	37	2	1	108	19	125	3.32e-40	130
MS.gene072495.t1	MTR_7g108600	56.481	108	40	3	4	109	15	117	4.55e-35	116
MS.gene072495.t1	MTR_3g084350	53.247	77	32	3	32	107	53	126	4.46e-20	79.3
MS.gene072495.t1	MTR_4g088685	56.250	64	26	1	46	107	44	107	2.24e-19	76.6
MS.gene072495.t1	MTR_6g060260	48.485	66	29	1	42	107	41	101	1.11e-15	67.0

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene072495.t1	AT4G15800	84.211	76	12	0	33	108	40	115	1.13e-43	138
MS.gene072495.t1	AT1G02900	60.909	110	39	2	1	109	14	120	2.14e-41	132
MS.gene072495.t1	AT3G16570	65.556	90	17	2	33	108	47	136	2.14e-33	113
MS.gene072495.t1	AT3G16570	65.556	90	17	2	33	108	47	136	2.14e-33	113
MS.gene072495.t1	AT3G16570	65.556	90	17	2	33	108	47	136	2.14e-33	113
MS.gene072495.t1	AT3G05490	81.034	58	11	0	51	108	61	118	2.77e-30	104
MS.gene072495.t1	AT2G33775	57.971	69	28	1	40	107	37	105	4.18e-23	86.3
MS.gene072495.t1	AT1G28270	55.072	69	30	1	40	107	37	105	1.73e-19	77.0
MS.gene072495.t1	AT4G13950	57.812	64	25	2	45	107	42	104	4.49e-18	73.6
MS.gene072495.t1	AT3G23805	60.714	56	20	2	53	107	55	109	1.14e-15	67.4
MS.gene072495.t1	AT5G67070	51.282	78	27	5	42	108	51	128	3.10e-12	58.9

Find 29 sgRNAs with CRISPR-Local

Find 30 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GCACTGCATCCACGGCTGTA+AGG	0.445471	7.3:-3824800	None:intergenic
AGTTTGATAATGAAATCAAC+AGG	0.461525	7.3:+3824663	MS.gene072495:CDS
CGCACCATAGCTGATGTACT+TGG	0.465658	7.3:-3824702	None:intergenic
CACTGCATCCACGGCTGTAA+GGG	0.481328	7.3:-3824799	None:intergenic
CAATTGCAGACCCGGTGCTC+AGG	0.488139	7.3:+3824772	MS.gene072495:CDS
CCTCCAGCTGCATCTACTGT+TGG	0.503199	7.3:-3824539	None:intergenic
GCAGCTGGAGGATTCGAACT+TGG	0.512453	7.3:+3824551	MS.gene072495:CDS
GAAGCACCACGGCGAGAACA+AGG	0.526826	7.3:-3824743	None:intergenic
GTAAGGGTTAGCCTGAGCAC+CGG	0.526971	7.3:-3824783	None:intergenic
ATCAGCGATGGAACCTTCGC+AGG	0.533132	7.3:-3824609	None:intergenic
TCTCCAACAGTAGATGCAGC+TGG	0.538924	7.3:+3824536	MS.gene072495:CDS
TCTTACTACAATTGCAGACC+CGG	0.540294	7.3:+3824764	MS.gene072495:CDS
AGGATTCGAACTTGGAGGAA+TGG	0.547500	7.3:+3824559	MS.gene072495:CDS
GCTGGAGGATTCGAACTTGG+AGG	0.550332	7.3:+3824554	MS.gene072495:CDS
TCGAACTTGGAGGAATGGAA+TGG	0.552877	7.3:+3824564	MS.gene072495:CDS
TCAGCTATGGTGCGCTTCAG+AGG	0.563424	7.3:+3824711	MS.gene072495:CDS
GCAGTGCTATTACGAGGTGC+AGG	0.566649	7.3:+3824816	MS.gene072495:CDS
TCGTAATAGCACTGCATCCA+CGG	0.577260	7.3:-3824808	None:intergenic
TAAGGGTTAGCCTGAGCACC+GGG	0.580885	7.3:-3824782	None:intergenic
TTGTAACATGCAATCAGCGA+TGG	0.586357	7.3:-3824621	None:intergenic
CAGGCTAACCCTTACAGCCG+TGG	0.595858	7.3:+3824791	MS.gene072495:CDS
ACGACCAAGTACATCAGCTA+TGG	0.604031	7.3:+3824698	MS.gene072495:CDS
AGCGATGGAACCTTCGCAGG+TGG	0.625016	7.3:-3824606	None:intergenic
AAGCACCACGGCGAGAACAA+GGG	0.626487	7.3:-3824742	None:intergenic
ACTGTCCCTTGTTCTCGCCG+TGG	0.647098	7.3:+3824737	MS.gene072495:CDS
CCAACAGTAGATGCAGCTGG+AGG	0.701563	7.3:+3824539	MS.gene072495:CDS
GCTGATTGCATGTTACAACA+AGG	0.712911	7.3:+3824626	MS.gene072495:CDS
GTGGATGCAGTGCTATTACG+AGG	0.733772	7.3:+3824810	MS.gene072495:CDS
AATTGTAGTAAGAAGCACCA+CGG	0.751980	7.3:-3824754	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	AGTTTGATAATGAAATCAAC+AGG	+	chr7.3:3824663-3824682	MS.gene072495:CDS	25.0%
!!	AATTGTAGTAAGAAGCACCA+CGG	-	chr7.3:3824757-3824776	None:intergenic	35.0%
	GCTGATTGCATGTTACAACA+AGG	+	chr7.3:3824626-3824645	MS.gene072495:CDS	40.0%
	TCTTACTACAATTGCAGACC+CGG	+	chr7.3:3824764-3824783	MS.gene072495:CDS	40.0%
	TTGTAACATGCAATCAGCGA+TGG	-	chr7.3:3824624-3824643	None:intergenic	40.0%
	ACGACCAAGTACATCAGCTA+TGG	+	chr7.3:3824698-3824717	MS.gene072495:CDS	45.0%
	AGGATTCGAACTTGGAGGAA+TGG	+	chr7.3:3824559-3824578	MS.gene072495:CDS	45.0%
	TCGAACTTGGAGGAATGGAA+TGG	+	chr7.3:3824564-3824583	MS.gene072495:CDS	45.0%
	TCGTAATAGCACTGCATCCA+CGG	-	chr7.3:3824811-3824830	None:intergenic	45.0%
	ACAAAAACAGCCACCTGCGA+AGG	+	chr7.3:3824596-3824615	MS.gene072495:CDS	50.0%
	CGCACCATAGCTGATGTACT+TGG	-	chr7.3:3824705-3824724	None:intergenic	50.0%
	TCTCCAACAGTAGATGCAGC+TGG	+	chr7.3:3824536-3824555	MS.gene072495:CDS	50.0%
!	GTGGATGCAGTGCTATTACG+AGG	+	chr7.3:3824810-3824829	MS.gene072495:CDS	50.0%
	AAGCACCACGGCGAGAACAA+GGG	-	chr7.3:3824745-3824764	None:intergenic	55.0%
	ATCAGCGATGGAACCTTCGC+AGG	-	chr7.3:3824612-3824631	None:intergenic	55.0%
	CACTGCATCCACGGCTGTAA+GGG	-	chr7.3:3824802-3824821	None:intergenic	55.0%
	CCAACAGTAGATGCAGCTGG+AGG	+	chr7.3:3824539-3824558	MS.gene072495:CDS	55.0%
	CCTCCAGCTGCATCTACTGT+TGG	-	chr7.3:3824542-3824561	None:intergenic	55.0%
	GCAGCTGGAGGATTCGAACT+TGG	+	chr7.3:3824551-3824570	MS.gene072495:CDS	55.0%
	GCTGGAGGATTCGAACTTGG+AGG	+	chr7.3:3824554-3824573	MS.gene072495:CDS	55.0%
	TCAGCTATGGTGCGCTTCAG+AGG	+	chr7.3:3824711-3824730	MS.gene072495:CDS	55.0%
!	GCAGTGCTATTACGAGGTGC+AGG	+	chr7.3:3824816-3824835	MS.gene072495:CDS	55.0%
!!	GTAAGGGTTAGCCTGAGCAC+CGG	-	chr7.3:3824786-3824805	None:intergenic	55.0%
!!	TAAGGGTTAGCCTGAGCACC+GGG	-	chr7.3:3824785-3824804	None:intergenic	55.0%
	ACTGTCCCTTGTTCTCGCCG+TGG	+	chr7.3:3824737-3824756	MS.gene072495:CDS	60.0%
	AGCGATGGAACCTTCGCAGG+TGG	-	chr7.3:3824609-3824628	None:intergenic	60.0%
	CAATTGCAGACCCGGTGCTC+AGG	+	chr7.3:3824772-3824791	MS.gene072495:CDS	60.0%
	CAGGCTAACCCTTACAGCCG+TGG	+	chr7.3:3824791-3824810	MS.gene072495:CDS	60.0%
	GCACTGCATCCACGGCTGTA+AGG	-	chr7.3:3824803-3824822	None:intergenic	60.0%
!	GAAGCACCACGGCGAGAACA+AGG	-	chr7.3:3824746-3824765	None:intergenic	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr7.3	gene	3824515	3824844	3824515	ID=MS.gene072495
chr7.3	mRNA	3824515	3824844	3824515	ID=MS.gene072495.t1;Parent=MS.gene072495
chr7.3	exon	3824515	3824844	3824515	ID=MS.gene072495.t1.exon1;Parent=MS.gene072495.t1
chr7.3	CDS	3824515	3824844	3824515	ID=cds.MS.gene072495.t1;Parent=MS.gene072495.t1

Gene Sequence	>MS.gene072495 ATGACAACGATGATGAGTTCTTCTCCAACAGTAGATGCAGCTGGAGGATTCGAACTTGGAGGAATGGAATGGATTCATCAAACAAAAACAGCCACCTGCGAAGGTTCCATCGCTGATTGCATGTTACAACAAGGTGAAGAAGAGTTTCAGTTTGATAATGAAATCAACAGGCGTATCTTAGCAACGACCAAGTACATCAGCTATGGTGCGCTTCAGAGGAACACTGTCCCTTGTTCTCGCCGTGGTGCTTCTTACTACAATTGCAGACCCGGTGCTCAGGCTAACCCTTACAGCCGTGGATGCAGTGCTATTACGAGGTGCAGGAGTTAA
Protein sequence	>MS.gene072495.t1 MTTMMSSSPTVDAAGGFELGGMEWIHQTKTATCEGSIADCMLQQGEEEFQFDNEINRRILATTKYISYGALQRNTVPCSRRGASYYNCRPGAQANPYSRGCSAITRCRS